CN204939455U - A kind of Novel anaerobic culture tank - Google Patents

A kind of Novel anaerobic culture tank Download PDF

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Publication number
CN204939455U
CN204939455U CN201520703297.8U CN201520703297U CN204939455U CN 204939455 U CN204939455 U CN 204939455U CN 201520703297 U CN201520703297 U CN 201520703297U CN 204939455 U CN204939455 U CN 204939455U
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conduit
tank
tank body
screw
anaerobic
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刘韩
陈雪
关宏
吕丽艳
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Qiqihar Medical University
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Qiqihar Medical University
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Abstract

本实用新型公开了一种新型厌氧培养罐,属于微生物仪器技术领域,罐体为一上面开口的玻璃圆柱体,顶端侧壁设有上盖铰接座和螺丝铰接座,盖体侧壁设有上盖连接块和螺丝卡块,盖体通过上方的上盖连接块与罐体的上盖铰接座通过铰轴铰接,盖体可紧密扣在罐体上,盖体与罐体接触处设有密封圈,羊角螺丝铰接在螺丝铰接座上,羊角螺丝旋入螺丝卡块,螺纹旋紧后将整个罐体内部密封。罐体内底部设有厌氧粉皿,厌氧粉皿上连接有一导管,导管为透明的带有刻度的导管,橡胶堵头设置在导管的下端,橡胶堵头顶部连接有一导杆,避免上盖密封过程中出现的意外导致培养失败,操作方便,且大大降低了厌氧菌培养失败的可能性。

The utility model discloses a novel anaerobic culture tank, which belongs to the technical field of microbial instruments. The tank body is a glass cylinder with an upper opening, the top side wall is provided with an upper cover hinge seat and a screw hinge seat, and the cover body side wall is provided with The upper cover connection block and the screw clamp block, the cover body is hinged with the upper cover hinge seat of the tank body through the upper cover connection block above through the hinge shaft, the cover body can be tightly buckled on the tank body, and the contact between the cover body and the tank body is provided with The sealing ring is hinged on the screw hinge seat by the horn screw, and the horn screw is screwed into the screw block, and the whole tank body is sealed after the thread is tightened. There is an anaerobic powder dish at the bottom of the tank, and a conduit is connected to the anaerobic powder dish. The conduit is a transparent conduit with scales. The rubber plug is set at the lower end of the conduit. Accidents in the sealing process lead to culture failure, the operation is convenient, and the possibility of anaerobic culture failure is greatly reduced.

Description

一种新型厌氧培养罐A new type of anaerobic culture tank

技术领域 technical field

本实用新型涉及一种新型厌氧培养罐,属于微生物仪器技术领域。 The utility model relates to a novel anaerobic culture tank, which belongs to the technical field of microbial instruments.

背景技术 Background technique

厌氧菌与人们的生活息息相关,实验室中经常要对厌氧菌进行培养。但厌氧菌生长要求在无氧条件下,实验中经常由于其培养环境造成厌氧菌的培养失败。因此在培养厌氧菌时需要消除其培养环境中的氧气。在各种除氧的方法中使用专门的厌氧菌培养设备来除氧是最有效和最广泛的方法。通常是用含钯的钯粒催化剂在厌氧培养罐内使氢气和氧气进行反应从而将氧除去。 Anaerobic bacteria are closely related to people's lives, and anaerobic bacteria are often cultivated in laboratories. However, the growth of anaerobic bacteria requires anaerobic conditions, and the cultivation of anaerobic bacteria often fails due to the culture environment in experiments. Therefore, it is necessary to eliminate the oxygen in the culture environment when cultivating anaerobic bacteria. Among the various deaeration methods, the use of specialized anaerobic bacteria culture equipment to deaeration is the most effective and extensive method. Usually, hydrogen and oxygen are reacted in an anaerobic culture tank using a palladium-containing palladium grain catalyst to remove oxygen.

厌氧培养罐是在进行微生物厌氧培养时用到的辅助仪器,在利用厌氧产气袋法进行厌氧培养时,由于其催化剂摆放与厌氧菌在各自器皿中层叠摆放,即使罐体气密性很好,但因为没有充分反应,也十分容易导致培养失败。加入过多钯粒又会占用厌氧菌空间,和造成不必要的浪费。专利号为201520071233.0的一种新型厌氧培养罐解决了上述问题,但在反复的使用过程中发现,该罐在化学反应开始后才能将上盖密封,这使得内部仍有可能存在氧气,导致培养失败,且上盖的密封方式繁琐复杂,费时费力。 The anaerobic culture tank is an auxiliary instrument used in the anaerobic culture of microorganisms. When the anaerobic gas bag method is used for anaerobic culture, because the catalyst is placed and the anaerobic bacteria are stacked in their respective containers, even if The airtightness of the tank is very good, but because of insufficient reaction, it is very easy to cause culture failure. Adding too much palladium particles will take up the space for anaerobic bacteria and cause unnecessary waste. A new type of anaerobic culture tank with patent number 201520071233.0 solves the above problems, but it is found during repeated use that the tank can only seal the upper cover after the chemical reaction starts, which makes it possible that there may still be oxygen inside, resulting in the cultivation of Failed, and the sealing method of the upper cover is complicated, time-consuming and labor-intensive.

发明内容 Contents of the invention

为了解决上述问题,本实用新型提供了一种新型厌氧培养罐,它能有效避免钯粒不能充分接触罐内气体与其反应而造成的厌氧培养失败,整个反应在罐体密封后开始,避免上盖密封过程中出现的意外导致培养失败,上盖采用卡扣式密封,结构简单,持久耐用,便于试验管理。 In order to solve the above problems, the utility model provides a new type of anaerobic culture tank, which can effectively avoid the failure of anaerobic culture caused by palladium particles not fully contacting the gas in the tank and reacting with it. The whole reaction starts after the tank body is sealed to avoid Accidents during the sealing process of the upper cover lead to culture failure. The upper cover adopts snap-fit sealing, which is simple in structure, durable and convenient for test management.

本实用新型解决其技术问题所采用的技术方案是:一种新型厌氧培养罐,包括罐体、培养皿架、厌氧粉皿和隔热网,罐体为一上面开口的玻璃圆柱体,顶端侧壁一侧设有上盖铰接座,另一侧设有螺丝铰接座,在其上面设有盖体,盖体侧壁一侧设有上盖连接块,另一侧设有螺丝卡块,盖体通过上方的上盖连接块与罐体的上盖铰接座通过铰轴铰接,盖体可紧密扣在罐体上,盖体与罐体接触处设有密封圈,羊角螺丝铰接在螺丝铰接座上,羊角螺丝旋入螺丝卡块,螺纹旋紧后将整个罐体内部密封。罐体内底部设有厌氧粉皿,厌氧粉皿上连接有一导管,导管为透明的带有刻度的导管,导管高度接近罐体且不高于罐体,在厌氧粉皿上设有一隔热网,在隔热网上设有培养皿架,培养皿架呈圆柱型框架,无封顶,外侧壁环形固定有数个钯粒环形储槽,钯粒环形储槽为一近似360度的环形槽,其开口处可使导管通过。其中,橡胶堵头设置在导管的下端,直径大于导管内壁直径,橡胶堵头顶部连接有一导杆,导杆在导管内部,高于导管,且高于罐体高度。 The technical solution adopted by the utility model to solve the technical problem is: a novel anaerobic culture tank, comprising a tank body, a culture dish rack, anaerobic powder dish and heat insulation net, the tank body is a glass cylinder with an upper opening, One side of the top side wall is provided with a hinged seat for the upper cover, and the other side is provided with a screw hinged seat, and a cover is provided on it. One side of the side wall of the cover is provided with a connecting block for the upper cover, and the other side is provided with a screw block. , the cover body is hinged with the upper cover hinge seat of the tank body through the upper cover connecting block above through the hinge shaft, the cover body can be tightly buckled on the tank body, a sealing ring is provided at the contact point between the cover body and the tank body, and the horn screw is hinged on the screw On the hinged seat, the claw screw is screwed into the screw block, and the whole tank body is sealed after the thread is tightened. There is an anaerobic powder dish at the bottom of the tank, and a conduit is connected to the anaerobic powder dish. The conduit is a transparent conduit with a scale. The heat net has a petri dish frame on the heat insulation net. The petri dish rack is a cylindrical frame without a cap. There are several ring-shaped palladium grain storage tanks fixed on the outer wall. The palladium grain ring storage tank is an approximately 360-degree annular groove. Its opening allows the conduit to pass through. Wherein, the rubber plug is arranged at the lower end of the conduit, and its diameter is larger than that of the inner wall of the conduit. A guide rod is connected to the top of the rubber plug, and the guide rod is inside the conduit, higher than the conduit, and higher than the height of the tank.

本实用新型的有益效果是:解决了现有厌氧培养罐中催化剂摆放与厌氧菌在各自器皿中层叠摆放,即使罐体气密性很好,但因为没有充分反应,也十分容易导致培养失败,加入过多钯粒又会占用厌氧菌空间,和造成不必要的浪费的问题。整个反应在罐体密封后开始,避免上盖密封过程中出现的意外导致培养失败,上盖采用卡扣式密封,操作方便,且大大降低了厌氧菌培养失败的可能性。 The beneficial effect of the utility model is: it solves the problem of placing catalysts in existing anaerobic culture tanks and placing anaerobic bacteria in layers in their respective containers. Cause culture to fail, add too much palladium particle and can take up the space of anaerobic bacteria again, and cause the problem of unnecessary waste. The whole reaction starts after the tank is sealed, so as to avoid accidents during the sealing process of the upper cover leading to culture failure. The upper cover is sealed with a buckle, which is easy to operate and greatly reduces the possibility of failure of anaerobic bacteria culture.

附图说明 Description of drawings

下面结合附图和具体实施方式对本实用新型进一步说明。 Below in conjunction with accompanying drawing and specific embodiment the utility model is further described.

图1是本实用新型上盖打开后的主视图。 Fig. 1 is the front view of the utility model after the upper cover is opened.

图2是本实用新型的主视图。 Fig. 2 is the front view of the utility model.

图3是本实用新型上盖打开后的俯视图。 Fig. 3 is a top view of the utility model after the upper cover is opened.

图4是本实用新型的俯视图。 Fig. 4 is a top view of the utility model.

图中标号: Labels in the figure:

1、导杆,2、导管,3、盖体,4、密封圈,5、钯粒环形储槽,6、培养皿架,7、罐体,8、隔热网,9、厌氧粉皿,10、上盖连接块,11、上盖铰接座,12、橡胶堵头,13、螺丝铰接座,14、羊角螺丝,15、螺丝卡块。 1. Guide rod, 2. Conduit, 3. Cover body, 4. Sealing ring, 5. Palladium particle annular storage tank, 6. Petri dish rack, 7. Tank body, 8. Heat insulation net, 9. Anaerobic powder dish , 10, upper cover connection block, 11, upper cover hinged seat, 12, rubber plug, 13, screw hinged seat, 14, claw screw, 15, screw block.

具体实施方式 detailed description

如图1-4所示,一种新型厌氧培养罐,包括罐体7、培养皿架6、厌氧粉皿9和隔热网8,罐体7为一上面开口的玻璃圆柱体,顶端侧壁一侧设有上盖铰接座11,另一侧设有螺丝铰接座13,在其上面设有盖体3,盖体3侧壁一侧设有上盖连接块10,另一侧设有螺丝卡块15,盖体3通过上方的上盖连接块10与罐体7的上盖铰接座11通过铰轴铰接,盖体3可紧密扣在罐体7上,盖体3与罐体7接触处设有密封圈4,羊角螺丝14铰接在螺丝铰接座13上,羊角螺丝14旋入螺丝卡块15,螺纹旋紧后将整个罐体内部密封。罐体7内底部设有厌氧粉皿9,厌氧粉皿9上连接有一导管2,导管2为透明的带有刻度的导管,导管2高度接近罐体7且不高于罐体7,在厌氧粉皿9上设有一隔热网8,在隔热网8上设有培养皿架6,培养皿架6呈圆柱型框架,无封顶,外侧壁环形固定有数个钯粒环形储槽5,钯粒环形储槽5为一近似360度的环形槽,其开口处可使导管2通过。其中,橡胶堵头12设置在导管2的下端,直径大于导管2内壁直径,橡胶堵头12顶部连接有一导杆1,导杆1在导管2内部,高于导管2,且高于罐体7高度。 As shown in Figure 1-4, a new type of anaerobic culture tank includes a tank body 7, a culture dish rack 6, anaerobic powder dish 9 and heat insulation net 8, and the tank body 7 is a glass cylinder with an opening on the top. One side of the side wall is provided with a loam cake hinged seat 11, and the other side is provided with a screw hinged seat 13, on which a cover body 3 is provided, and one side of the side wall of the cover body 3 is provided with an upper cover connecting block 10, and the other side is provided with a loam cake connecting block 10. There is a screw block 15, and the cover body 3 is hinged with the upper cover hinge seat 11 of the tank body 7 through the upper cover connecting block 10 above through the hinge shaft, and the cover body 3 can be tightly buckled on the tank body 7, and the cover body 3 and the tank body 7. A sealing ring 4 is provided at the contact, and the claw screw 14 is hinged on the screw hinge seat 13, and the claw screw 14 is screwed into the screw block 15, and the whole tank body is sealed inside after the screw thread is tightened. An anaerobic powder dish 9 is provided at the inner bottom of the tank body 7, and a conduit 2 is connected to the anaerobic powder dish 9. The conduit 2 is a transparent conduit with a scale, and the height of the conduit 2 is close to the tank body 7 and not higher than the tank body 7. A heat insulation net 8 is arranged on the anaerobic powder dish 9, and a culture dish rack 6 is arranged on the heat insulation net 8. The culture dish rack 6 is a cylindrical frame without a cap, and several ring-shaped palladium particle ring storage tanks are fixed on the outer wall. 5. The palladium particle annular storage tank 5 is an approximately 360-degree annular groove, and the opening can allow the conduit 2 to pass through. Wherein, the rubber plug 12 is arranged at the lower end of the conduit 2, and its diameter is greater than the diameter of the inner wall of the conduit 2. A guide rod 1 is connected to the top of the rubber plug 12, and the guide rod 1 is inside the conduit 2, higher than the conduit 2, and higher than the tank body 7. high.

通过导杆1将橡胶堵头12嵌入导管2底部,将水倒入导管2内,至相应刻度。只有将上盖3盖在罐体7上,用羊角螺丝14锁紧螺丝卡块15罐体7密封时,盖子3内壁顶住导杆1使橡胶堵头12落下,将水流入厌氧粉皿,确保了整个反应均在密闭罐体中进行。 Insert the rubber plug 12 into the bottom of the conduit 2 through the guide rod 1, and pour water into the conduit 2 to the corresponding scale. Only when the upper cover 3 is put on the tank body 7, and the screw block 15 is locked with the horn screw 14, when the tank body 7 is sealed, the inner wall of the cover 3 will withstand the guide rod 1 to make the rubber plug 12 drop, and the water will flow into the anaerobic powder dish , to ensure that the entire reaction is carried out in a closed tank.

Claims (3)

1.一种新型厌氧培养罐,包括罐体(7)、培养皿架(6)、厌氧粉皿(9)和隔热网(8),其特征在于:罐体(7)为一上面开口的玻璃圆柱体,顶端侧壁一侧设有上盖铰接座(11),另一侧设有螺丝铰接座(13),在其上面设有盖体(3),盖体(3)侧壁一侧设有上盖连接块(10),另一侧设有螺丝卡块(15),盖体(3)通过上方的上盖连接块(10)与罐体(7)的上盖铰接座(11)通过铰轴铰接,盖体(3)可紧密扣在罐体(7)上,盖体(3)与罐体(7)接触处设有密封圈(4),羊角螺丝(14)铰接在螺丝铰接座(13)上,羊角螺丝(14)旋入螺丝卡块(15),螺纹旋紧后将整个罐体内部密封;罐体(7)内底部设有厌氧粉皿(9),厌氧粉皿(9)上连接有一导管(2),导管(2)为透明的带有刻度的导管,导管(2)高度接近罐体(7)且不高于罐体(7),在厌氧粉皿(9)上设有一隔热网(8),在隔热网(8)上设有培养皿架(6),培养皿架(6)呈圆柱型框架,无封顶,外侧壁环形固定有数个钯粒环形储槽(5),钯粒环形储槽(5)为一近似360度的环形槽,其开口处可使导管(2)通过;其中,橡胶堵头(12)设置在导管(2)的下端,直径大于导管(2)内壁直径,橡胶堵头(12)顶部连接有一导杆(1),导杆(1)在导管(2)内部,高于导管(2),且高于罐体(7)高度。 1. A new type of anaerobic culture tank, including tank body (7), culture dish rack (6), anaerobic powder dish (9) and heat insulation net (8), characterized in that: tank body (7) is a A glass cylinder with an upper opening, one side of the top side wall is provided with an upper cover hinged seat (11), the other side is provided with a screw hinged seat (13), and a cover (3) is arranged on it, and the cover (3) One side of the side wall is provided with an upper cover connection block (10), and the other side is provided with a screw block (15), and the cover body (3) is connected with the upper cover of the tank body (7) through the upper upper cover connection block (10) The hinged seat (11) is hinged by the hinge shaft, the cover (3) can be fastened tightly on the tank (7), the contact between the cover (3) and the tank (7) is provided with a sealing ring (4), and the claw screw ( 14) Hinged on the screw hinge seat (13), the claw screw (14) is screwed into the screw block (15), and the whole tank body is sealed after the thread is tightened; the inner bottom of the tank body (7) is equipped with an anaerobic powder dish (9), the anaerobic powder dish (9) is connected with a conduit (2), the conduit (2) is a transparent conduit with a scale, the height of the conduit (2) is close to the tank (7) and not higher than the tank ( 7), a heat insulation net (8) is provided on the anaerobic powder dish (9), a petri dish rack (6) is arranged on the heat insulation net (8), and the petri dish rack (6) is a cylindrical frame, without The top is closed, and several ring-shaped storage tanks (5) of palladium grains are fixed on the outer wall. The ring-shaped storage tank (5) of palladium grains is an approximately 360-degree annular groove, and the opening can allow the conduit (2) to pass through; among them, the rubber plug (12) Set at the lower end of the conduit (2), the diameter is larger than the diameter of the inner wall of the conduit (2), the top of the rubber plug (12) is connected with a guide rod (1), the guide rod (1) is inside the conduit (2), higher than The conduit (2) is higher than the height of the tank body (7). 2.根据权利要求1所述的一种新型厌氧培养罐,其特征在于:所述的导杆(1)与橡胶堵头(12)一体化,橡胶堵头(12)直径大于导管(2)内壁直径,导杆(1)高度高于导管(2)且高于罐体(7)。 2. A new type of anaerobic culture tank according to claim 1, characterized in that: the guide rod (1) is integrated with the rubber plug (12), and the diameter of the rubber plug (12) is larger than that of the conduit (2 ) inner wall diameter, the height of the guide rod (1) is higher than the conduit (2) and higher than the tank body (7). 3.根据权利要求1所述的一种新型厌氧培养罐,其特征在于:所述的导管(2)为带有刻度的透明导管,高度接近罐体(7)内部高度,且低于罐体(7)内部高度。 3. A new type of anaerobic culture tank according to claim 1, characterized in that: the conduit (2) is a transparent conduit with scales, the height is close to the internal height of the tank body (7), and is lower than the tank body (7). body (7) internal height.
CN201520703297.8U 2015-09-13 2015-09-13 A kind of Novel anaerobic culture tank Expired - Fee Related CN204939455U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106190799A (en) * 2016-08-24 2016-12-07 陈学红 A kind of microbiological anaerobic is cultivated with cultivating bottle
CN106222083A (en) * 2016-08-18 2016-12-14 袁宇志 Intelligent pair of gas culture tank control system
CN107937245A (en) * 2017-11-21 2018-04-20 安徽天下福酒业有限公司 Three-dimensional kiln pond is sealed by fermentation device
CN111139174A (en) * 2020-01-15 2020-05-12 辽东学院 Mixed microorganism culture device

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106222083A (en) * 2016-08-18 2016-12-14 袁宇志 Intelligent pair of gas culture tank control system
CN106190799A (en) * 2016-08-24 2016-12-07 陈学红 A kind of microbiological anaerobic is cultivated with cultivating bottle
CN107937245A (en) * 2017-11-21 2018-04-20 安徽天下福酒业有限公司 Three-dimensional kiln pond is sealed by fermentation device
CN111139174A (en) * 2020-01-15 2020-05-12 辽东学院 Mixed microorganism culture device
CN111139174B (en) * 2020-01-15 2023-08-29 辽东学院 Mixed microorganism culture apparatus

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