CN1324129C - Multiple strain microorganism ferment production method and its uses in cow fine fodder - Google Patents
Multiple strain microorganism ferment production method and its uses in cow fine fodder Download PDFInfo
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- CN1324129C CN1324129C CNB2004100376139A CN200410037613A CN1324129C CN 1324129 C CN1324129 C CN 1324129C CN B2004100376139 A CNB2004100376139 A CN B2004100376139A CN 200410037613 A CN200410037613 A CN 200410037613A CN 1324129 C CN1324129 C CN 1324129C
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- 244000005700 microbiome Species 0.000 title abstract description 6
- 238000004519 manufacturing process Methods 0.000 title description 3
- 235000013336 milk Nutrition 0.000 claims abstract description 52
- 239000008267 milk Substances 0.000 claims abstract description 52
- 210000004080 milk Anatomy 0.000 claims abstract description 52
- 238000000855 fermentation Methods 0.000 claims abstract description 44
- 230000004151 fermentation Effects 0.000 claims abstract description 44
- 241000894006 Bacteria Species 0.000 claims abstract description 24
- 239000000835 fiber Substances 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000010902 straw Substances 0.000 claims abstract description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 36
- 230000001580 bacterial effect Effects 0.000 claims description 34
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 30
- 239000000843 powder Substances 0.000 claims description 30
- 239000007858 starting material Substances 0.000 claims description 29
- 229910052700 potassium Inorganic materials 0.000 claims description 27
- 239000001888 Peptone Substances 0.000 claims description 24
- 108010080698 Peptones Proteins 0.000 claims description 24
- 235000019319 peptone Nutrition 0.000 claims description 24
- 230000000813 microbial effect Effects 0.000 claims description 21
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 18
- 239000008103 glucose Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 241000186428 Propionibacterium freudenreichii Species 0.000 claims description 15
- 240000005384 Rhizopus oryzae Species 0.000 claims description 15
- 235000013752 Rhizopus oryzae Nutrition 0.000 claims description 15
- 229930006000 Sucrose Natural products 0.000 claims description 15
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 15
- 239000005720 sucrose Substances 0.000 claims description 15
- 229920001817 Agar Polymers 0.000 claims description 12
- 241000193749 Bacillus coagulans Species 0.000 claims description 12
- 241000985535 Penicillium decumbens Species 0.000 claims description 12
- 229920002472 Starch Polymers 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 229940054340 bacillus coagulans Drugs 0.000 claims description 12
- 235000005822 corn Nutrition 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 239000008107 starch Substances 0.000 claims description 12
- 235000019698 starch Nutrition 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 241000228245 Aspergillus niger Species 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- 241000186221 Cellulosimicrobium cellulans Species 0.000 claims description 9
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 9
- 235000001006 Saccharomyces cerevisiae var diastaticus Nutrition 0.000 claims description 9
- 244000206963 Saccharomyces cerevisiae var. diastaticus Species 0.000 claims description 9
- 241000588902 Zymomonas mobilis Species 0.000 claims description 9
- 229940041514 candida albicans extract Drugs 0.000 claims description 9
- 229910001873 dinitrogen Inorganic materials 0.000 claims description 9
- 235000015099 wheat brans Nutrition 0.000 claims description 9
- 239000012138 yeast extract Substances 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000011573 trace mineral Substances 0.000 claims description 7
- 235000013619 trace mineral Nutrition 0.000 claims description 7
- 239000004254 Ammonium phosphate Substances 0.000 claims description 6
- 244000063299 Bacillus subtilis Species 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 6
- 229910000148 ammonium phosphate Inorganic materials 0.000 claims description 6
- 235000019289 ammonium phosphates Nutrition 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 6
- 239000011707 mineral Substances 0.000 claims description 6
- 235000010755 mineral Nutrition 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000012452 mother liquor Substances 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- 240000009087 Crescentia cujete Species 0.000 claims description 5
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- 241000196324 Embryophyta Species 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 5
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 235000009797 Lagenaria vulgaris Nutrition 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
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- 239000011780 sodium chloride Substances 0.000 claims description 4
- 244000025254 Cannabis sativa Species 0.000 claims description 3
- 241000186318 Cellulomonas biazotea Species 0.000 claims description 3
- 241000186220 Cellulomonas flavigena Species 0.000 claims description 3
- 229920002488 Hemicellulose Polymers 0.000 claims description 3
- 244000285963 Kluyveromyces fragilis Species 0.000 claims description 3
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 claims description 3
- 241000186660 Lactobacillus Species 0.000 claims description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 3
- 241000500881 Lepisma Species 0.000 claims description 3
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 235000019262 disodium citrate Nutrition 0.000 claims description 3
- 239000002526 disodium citrate Substances 0.000 claims description 3
- CEYULKASIQJZGP-UHFFFAOYSA-L disodium;2-(carboxymethyl)-2-hydroxybutanedioate Chemical compound [Na+].[Na+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O CEYULKASIQJZGP-UHFFFAOYSA-L 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 238000011177 media preparation Methods 0.000 claims description 3
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- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
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- 235000020183 skimmed milk Nutrition 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
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- 238000012549 training Methods 0.000 claims description 3
- 230000006651 lactation Effects 0.000 abstract description 5
- 239000003205 fragrance Substances 0.000 abstract description 4
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- 230000036528 appetite Effects 0.000 abstract description 3
- 235000019789 appetite Nutrition 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract 1
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- 230000000369 enteropathogenic effect Effects 0.000 abstract 1
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- 235000013305 food Nutrition 0.000 description 3
- 210000004767 rumen Anatomy 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
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- 230000003203 everyday effect Effects 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- -1 or adding zymin Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
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- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
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- 108010002430 hemicellulase Proteins 0.000 description 1
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- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000021243 milk fat Nutrition 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The present invention relates to a method for preparing multiple strain microorganism ferment, and an application of the multiple strain microorganism ferment to milk cow concentrated feed, which belongs to the field of microorganism fermentation in biological engineering. The present invention adopts multiple microorganism strains which are proportionally combined to be made into ferment, and a finished product is prepared by that raw materials and straw are mixed evenly and blended with activated ferment to be put in a container for fermentation. Feed prepared from the ferment of the present invention raises the daily average milk yield of a milk cow by 0.5 to 1Kg, and extends a lactation period. By using the feed, a postpartum milk cow recovers fast so as to turn into lactation peak time in advance, digestive absorption is rapid, and the propagation of enteropathogenic bacteria is restrained. The leaven has unique sour fragrance which promotes the appetite of the milk cow, so that the eating speed of the milk cow is increased, the residue of feed is avoided, and a feed conversion rate is raised by 15%. Analysis of the feed shows that crude protein is raised by 20% to 25%, coarse fibers are lowered by 10% to 15%, feed cost is greatly reduced, and the benefit of an enterprise is obvious. A product of the present invention can accord with the requirements of green feed and realize safe feeding.
Description
One, technical field:
The present invention relates to a kind of making method of many bacterial classifications microbial starter culture, belong to microbial fermentation field in the biotechnology.
Two, background technology:
At present, research and development for cow concentrated feed, only limit to corn, wheat bran, the mixing of grouts and fish meal, meat meal tankage, feather meal, the ratio of zein, or adding zymin, mineral element and antibiotic formulating, cause in the prescription energy to have a surplus, protein feed single amino acid mismatch, mineral substance, trace element and VITAMIN famine, so that cause the transformation efficiency of feed low, milk yield, the protein ratio of milk fat content is low, the cow rumen oxypathy, the Nutrition and Metabolism complications is higher, and milk cow three fat mortalities are high and utilize the time limit short, influenced the performance of milk cow production potential.And feed cost height.
Three, summary of the invention:
The purpose of invention is to provide a kind of resistance against diseases strong, obviously reduces cow rumen oxypathy and diarrhoea phenomenon, prolongs the lactation time, has improved the making method of the low many bacterial classifications microbial starter culture of dairy fat content and cost and the application in cow concentrated feed thereof.
The object of the present invention is achieved like this:
Many bacterial classifications microbial starter culture making step is as follows:
Spawn culture: (1) former slant strains: 1) 8 kinds of bacteriums: dinitrogen cellulomonas cartae (Cellulomonasbiazotea); Produce yellowish fiber Zymomonas mobilis (Cellulomonas flavigena); Subtilis 10264 (Bacillus subtilis); Bacillus coagulans (Bacillus coagulans); Lactobacterium acidophilum (Lactobacillus acidophilus); Starch milk bacillus (Lactobacillus amylopHllus); Propionibacterium freudenreichii (propionibacterium freudenreichii), subtilis TQ13055 (Bacillus subtilis);
2) 3 primary yeast bacterium: Candida utilis (Candida utilis); Saccharomyces diastaticus (Saccharomyces diastaticus); Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae);
3) 3 kinds of moulds: Penicillium decumbens (Penicillium decumbens); Rhizopus oryzae (Rhizopusoryzae); Aspergillus niger (Aspergillus niger);
(2) purebred separation: get 1 ring from former slant strains and put into and killed bacterium and granulated glass sphere was housed and the triangular flask of 100ml water shakes up, with aseptic technique, get the plate that 1ml puts into sterilization and substratum is housed with aseptic straw, with aseptic glass slicker with bacterium liquid drawout, and with this scraper scrape second, third continuously, the 4th, the 5th identical plate is different dilutions and separates plate, put into 25-38 ℃ of insulation can and cultivate, the single bacterium colony that grows in the plate is inserted slant medium continue to put into after cultivation grows up to 4 ℃ of refrigerators preservations;
(3) liquid and slant culture: the dinitrogen cellulomonas cartae, produce the yellowish fiber Zymomonas mobilis, with straw powder substratum: straw 3-5%, boil added in 10 minutes yeast powder a little, natural pH;
Subtilis 10264, subtilis TQ13055, beef-protein medium: extractum carnis 0.5%, peptone 1%, sodium-chlor 0.5%, pH7.2-7.6, not adding agar is liquid nutrient medium, adding 1.5-2% agar is slant medium, use after 125 ℃ of sterilizations, the rest may be inferred;
Bacillus coagulans: yeast powder 0.8%, peptone 1%, glucose 0.7%, potassium primary phosphate 0.18%, sal epsom 0.11%, pH 7.0-7.2;
Propionibacterium freudenreichii: yeast powder 0.8%, peptone 1%, sucrose 1.5%, ammonium phosphate 0.4-0.6%, pH 6.8-7.0;
Lactobacterium acidophilum: skim-milk 10%, sugar 5%, natural pH, temperature: 38-39 ℃, cultivated 4-8 hour;
Starch milk bacillus: yeast extract paste 1%, peptone 1.0%, (NH
4)
2SO
40.5%, sucrose 2.0%, pH 7.2-7.4;
Fermention medium: dinitrogen cellulomonas cartae, product yellowish fiber Zymomonas mobilis: grass meal 2%, wheat bran 0.5%, yeast powder 0.5%; PH 7.2-7.4, temperature: 28-30 ℃, cultivated 20-22 hour;
Subtilis 10264 TQ13055: beef-protein medium: extractum carnis 0.5%, peptone 1%, sodium-chlor 0.5%, glucose 0.2%, pH 7.2-7.6; Temperature: 25-30 ℃, time: 22-24 hour;
Bacillus coagulans: yeast extract paste 1.0%, peptone 1.0%, glucose 0.6%, potassium primary phosphate 0.2%, manganous sulfate 0.1%, sal epsom 0.11%, pH 7.0; Temperature: 25-30 ℃, time 22-24 hour;
Propionibacterium freudenreichii: corn steep liquor 0.5%, glucose 0.2%, ammonium phosphate 0.02%, K
2HPO
40.08%, getting concentration is 0.1% CoCl
20.04ml, aquae destillata 100ml, pH 6.8-7.0, temperature: 25-30, time: 24-28 hour;
Lactobacterium acidophilum: peptone 1.0%, extractum carnis 1.0%, yeast extract paste 0.5%, glucose 2%, K
2HPO
40.2%, sodium-acetate 0.5%, disodium citrate 0.2%, MgSO
47H
2O 0.2%, MnSO
44H
2O 0.1%, milk powder 1%, tween 80 .1%, pH 6.0-6.5, temperature: 38-39 ℃, cultivated 4-8 hour;
Starch milk bacillus: Zulkovsky starch 2.0%, MgSO
47H
2O 0.05%, KNO
30.1%, FeSO
40.001%, NaCl 0.05%, K
2HPO
40.05%, 7.2-7.4, temperature: 25-30 ℃, time: 26-28 hour;
Yeast: Candida utilis, saccharomyces diastaticus slant medium: peptone 1.0%, yeast powder 0.5%, glucose 2.0%, pH 6.5 or natural pH;
The yeast saccharomyces cerevisiae slant medium: 5% corn hydrolyzed solution adds MgSO
47H
2O 0.3%, K
2HPO
40.15%, CaCO
30.5%, yeast powder 0.5%, pH 5-6, time: 24 hours;
Mould: Penicillium decumbens slant medium: potato 20%, sucrose 2%, K
2HPO
40.3%, MgSO
47H
2O0.15%, agar 2.0%, pH 6.0;
Fermention medium: NaNO
30.3%, KCl 0.05%, K
2HPO
40.1%, FeSO
40.001%, sucrose 3%, MgSO
47H
2O 0.05%, pH 6.7, time: 48-56 hour;
The same Penicillium decumbens of Rhizopus oryzae slant medium:
Rhizopus oryzae fermention medium: Vogel ' s mother liquor 4%, hemicellulose 1%, trace element 0.01%, temperature: 28-30, time: 2 days, micro-component: 2NHCl 5ml, FeSO
40.25g, MnSO
4H
2O 0.98g, ZnCl
20.83g, CoCl
21.0g, aquae destillata 100ml;
Aspergillus niger slant medium: NaNO
30.3%, KCl 0.05%, K
2HPO
40.1%, FeSO
40.001%, sucrose 3.0%, MgSO
47H
2O 0.05%, agar 2.0%, pH 6.7, time: 28-32 hour;
Fermention medium: Vogel ' s mother liquor 4%, Microcrystalline Cellulose 1%, trace element 0.01%, micro-component: 2NHCl 5ml, FeSO
40.25g, MnSO
4H
2O 0.98g, ZnCl
20.83g, CoCl
21.0g, aquae destillata 100ml, its same Rhizopus oryzae of filling a prescription;
(4) shake pipe: get an articulating and go into sterilization and be equipped with in the 10ml liquid nutrient medium test tube from long good slant strains, shaking table was cultivated 1-2 days under 25-38 ℃ of condition, except that Lactobacterium acidophilum, the static cultivation of propionibacterium freudenreichii;
(5) triangular flask is cultivated: pack into after the fermention medium 100-120ml sterilization with the 500ml triangular flask, pour in the triangular flask the good test tube that shakes training into 25-38 ℃ under spirit lamp and shake and trained 1-2 days;
(6) go up a jar enlarged culturing: after the fermention medium preparation, autoclaving 0.1MPa, time: 20-50 minute, be cooled to 30-38 ℃ and use aseptic technique, cultured triangular flask bacterial classification is inserted in the jar, inoculum size is 2-10%, under 25-38 ℃ of condition, ventilate or stuffy cultivation, ventilate and cultivate 8-12m
3/ h, tank pressure 0.01-0.08Mpa, incubation time 18-24 hour, stuffy need stirred once every 4-5 hour.
The proportioning of each bacterial classification: with cultured bacterium by 1: 1: 1: 1: 1: 1: fit in the fermentation using bacteria agent at 1: 1, cultured yeast is pressed 1: 1: 0.5 synthetic yeast starter, cultured mould was made the mold fermentation agent by 1: 1: 1, three kinds of starters were pressed 0.5: 1: 1; 0.5: 2: 1; 0.5: 3: 1; 1: 0.5: 1; 1: 1: 1; 1: 2: 1; 1: 3: 1; 2: 0.5: 1; 2: 1: 1; Be made into the starter of different ratio bacterial classifications at 2: 3: 1 as the concentrated feed fermentation;
With farm crop corn 400-600Kg, certain herbaceous plants with big flowers cake 150-200Kg, calabash fiber crops cake 50-150Kg, soya-bean cake 50-150Kg, wheat bran 30-80Kg, straw powder 30-50Kg, mineral substance 20-50Kg, after the urea 2.5-10Kg raw material pulverizing, after 20 orders sieve, cooperation is mixed thoroughly, amount of water is 40-50%, added behind the water material moistening 1 hour, the raw material that profit is good adds the starter that 100L mixes by 1000Kg, the blending ratio of raw material and water is 1: 0.5-1, mix back pack fermentation, every packed 20-40kg, fermentation time: decide according to bacterial classification, spontaneous fermentation 4-7 in summer days, in sky 12-17 days spring and autumn, in 1-2 month winter, be finished product after the fermentation.
The present invention has following characteristics: 1, produce organic acid and be mainly lactic acid, participate in organism metabolism directly, energize; 2, noresidue, toxicological harmless, do not develop immunity to drugs; 3, prevent and treat diarrhoea; 4, reduce pH value in the enteron aisle, suppress the spoilage organism growth, the activator enzyme; 5, improve immunizing power and keep enteron aisle ecological steadily; 6, increase the output and the quality of milk; 7, improve the feed local flavor; 8, whet the appetite, improve food-intake.
The invention effect:
Many bacterial classifications microorganism species rolls up the cow rumen probiotic bacterium.Make microbial fermentation milk cow concentrated feed have fragrance, suitability is good, does not have obviously to reload the phase, to the not significantly influence of milk yield of milk cow.Milk cow physique obviously improves, and shows as milk cow hair color gloss, and ight soil is normal, the lactation stable yield of milk cow, and the milk production of cow of feeding through general feeds daily improves 0.5-1Kg, and helps prolonging lactation period.Promote milk cow to recover postpartum, general milk cow needs could recover more than the two weeks postpartum, and the milk cow of many bacterial classifications microbial fermentation milk cow concentrated feed of feeding only needed 3-8 days can recover physique, entered milk secreting period in advance.
Many bacterial classifications microbial fermentation milk cow concentrated feed has reached the standard of nuisanceless green feed.All need add additives such as hormone and microbiotic in the general feeds, in the long-term accumulation milk cow body, have a strong impact on the edible safety of ox, and has the beneficial bacteria of intestinal tract group in the organism of fermentation flora, without any side effects to milk cow, and can absorb by promoting digestion, suppress pathogen enterobacteria and grow, reach and give epidemic prevention and strengthen milk cow resistibility and effect.In feeding experiment, though do not add additives such as microbiotic at many bacterial classifications microbial fermentation milk cow concentrated feed, edible experimental group milk cow never sees bloated tripe, diarrhoea phenomenon in experiment periods.And bloated tripe of ox and symptom of diarrhea often appear in control group.Mazoitis is the common disease of milk cow, and this disease can obviously influence the milk yield of milk cow, and needs to use microbiotic, and mazoitis does not take place the experiment milk cow during many bacterial classifications microbial fermentation milk cow concentrated feed of feeding.So product of the present invention can reach the requirement of green feed, realize safe feeding.
The raising efficiency of feed utilization of saving food.In many bacterial classifications microbial fermentation milk cow concentrated feed, substitute the grain of 3%-5% with the straw powder, realized the purpose of joint grain, and fermented feed has unique sour fragrance and flavour, increase the appetite of milk cow, eating speed obviously improves, the time of searching for food has shortened to 3-10 minute, can all fermented feed of feeding foods are clean, do not have residual, improve efficiency of feed utilization 15%, reduce feed waste, and through feed is analyzed: crude protein improves 20%-25%, and robust fibre reduces 10%-15%, feed cost reduces greatly, and the performance of enterprises is obvious.
Many bacterial classifications microbial fermentation milk cow concentrated feed has tangible economic benefit:
(1) for the user, the price of at present general concentrated feed is at 0.6-0.8 unit/500 grams, and the cost of many bacterial classifications microbial fermentation milk cow concentrated feed of the identical energy of feeding is at 0.5 yuan/500 grams.The on average edible 10Kg ferment essence feed of every cow head every day can be saved cost 2-6 unit.And many bacterial classifications microbial fermentation milk cow concentrated feed of feeding, milk cow day volume increase 0.5-1Kg fresh milk, 274.5-509 unit increases income in year.Two add up in every cow heads the 1004.5-2179 unit that can increase income every year, and economic benefit is very obvious.
(2) to the producer, but by ten thousand yuan of 100 tons of yield meter extra earnings 2-6 every day, if by dropping into 1,000 ten thousand yuan, the payback period, with the most conservative calculating less than 2 years, benefit was more obvious.
Four, embodiment: (most preferred embodiment of the present invention)
The making step of starter is as follows:
Former slant strains → purebred separation → access inclined-plane → shake pipe cultivations → triangular flask enlarged culturing → go up jar cultivates → with 14 kinds of strain fermentation products by different mixed even → starter.
The making step of starter is as follows:
Many bacterial classifications microbial starter culture making step is as follows:
Spawn culture: (1) former slant strains: 1) 8 kinds of bacteriums: dinitrogen cellulomonas cartae (Cellulomonasbiazotea); Produce yellowish fiber Zymomonas mobilis (Cellulomonas flavigena); Subtilis 10264 (Bacillus subtilis); Bacillus coagulans (Bacillus coagulans); Lactobacterium acidophilum (Lactobacillus acidophilus); Starch milk bacillus (Lactobacillus amylopHllus); Propionibacterium freudenreichii (propionibacterium freudenreichii), subtilis TQ13055 (Bacillus subtilis);
2) 3 primary yeast bacterium: Candida utilis (Candida utilis); Saccharomyces diastaticus (Saccharomyces diastaticus); Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae);
3) 3 kinds of moulds: Penicillium decumbens (Penicillium decumbens); Rhizopus oryzae (Rhizopusoryzae); Aspergillus niger (Aspergillus niger);
(2) purebred separation: get 1 ring from former slant strains and put into and killed bacterium and granulated glass sphere was housed and the triangular flask of 100ml water shakes up, with aseptic technique, get the plate that 1ml puts into sterilization and substratum is housed with aseptic straw, with aseptic glass slicker with bacterium liquid drawout, and with this scraper scrape second, third continuously, the 4th, the 5th identical plate is different dilutions and separates plate, put into 25-38 ℃ of insulation can and cultivate, the single bacterium colony that grows in the plate is inserted slant medium continue to put into after cultivation grows up to 4 ℃ of refrigerators preservations;
(3) liquid and slant culture: the dinitrogen cellulomonas cartae, produce the yellowish fiber Zymomonas mobilis, with straw powder substratum: straw 3-5%, boil added in 10 minutes yeast powder a little, natural pH;
Subtilis 10264, subtilis TQ13055, beef-protein medium: extractum carnis 0.5%, peptone 1%, sodium-chlor 0.5%, pH7.2-7.6, not adding agar is liquid nutrient medium, adding 1.5-2% agar is slant medium, use after 125 ℃ of sterilizations, the rest may be inferred;
Bacillus coagulans: yeast powder 0.8%, peptone 1%, glucose 0.7%, potassium primary phosphate 0.18%, sal epsom 0.11%, pH 7.0-7.2;
Propionibacterium freudenreichii: yeast powder 0.8%, peptone 1%, sucrose 1.5%, ammonium phosphate 0.4-0.6%, pH 6.8-7.0;
Lactobacterium acidophilum: skim-milk 10%, sugar 5%, natural pH, temperature: 38-39 ℃, cultivated 4-8 hour;
Starch milk bacillus: yeast extract paste 1%, peptone 1.0%, (NH
4)
2SO
40.5%, sucrose 2.0%, pH 7.2-7.4;
Fermention medium: dinitrogen cellulomonas cartae, product yellowish fiber Zymomonas mobilis: grass meal 2%, wheat bran 0.5%, yeast powder 0.5%; PH 7.2-7.4, temperature: 28-30 ℃, cultivated 20-22 hour;
Subtilis 10264 TQ13055: beef-protein medium: extractum carnis 0.5%, peptone 1%, sodium-chlor 0.5%, glucose 0.2%, pH 7.2-7.6; Temperature: 25-30 ℃, time: 22-24 hour;
Bacillus coagulans: yeast extract paste 1.0%, peptone 1.0%, glucose 0.6%, potassium primary phosphate 0.2%, manganous sulfate 0.1%, sal epsom 0.11%, pH 7.0; Temperature: 25-30 ℃, time 22-24 hour;
Propionibacterium freudenreichii: corn steep liquor 0.5%, glucose 0.2%, ammonium phosphate 0.02%, K
2HPO
40.08%, getting concentration is 0.1% CoCl
20.04ml, aquae destillata 100ml, pH 6.8-7.0, temperature: 25-30, time: 24-28 hour;
Lactobacterium acidophilum: peptone 1.0%, extractum carnis 1.0%, yeast extract paste 0.5%, glucose 2%, K
2HPO
40.2%, sodium-acetate 0.5%, disodium citrate 0.2%, MgSO
47H
2O 0.2%, MnSO
44H
2O 0.1%, milk powder 1%, tween 80 .1%, pH 6.0-6.5, temperature: 38-39 ℃, cultivated 4-8 hour;
Starch milk bacillus: Zulkovsky starch 2.0%, MgSO
47H
2O 0.05%, KNO
30.1%, FeSO
40.001%, NaCl 0.05%, K
2HPO
40.05%, 7.2-7.4, temperature: 25-30 ℃, time: 26-28 hour;
Yeast: Candida utilis, saccharomyces diastaticus slant medium: peptone 1.0%, yeast powder 0.5%, glucose 2.0%, pH 6.5 or natural pH;
The yeast saccharomyces cerevisiae slant medium: 5% corn hydrolyzed solution adds MgSO
47H
2O 0.3%, K
2HPO
40.15%, CaCO
30.5%, yeast powder 0.5%, pH 5-6, time: 24 hours;
Mould: Penicillium decumbens slant medium: potato 20%, sucrose 2%, K
2HPO
40.3%, MgSO
47H
2O0.15%, agar 2.0%, pH 6.0;
Fermention medium: NaNO
30.3%, KCl 0.05%, K
2HPO
40.1%, FeSO
40.001%, sucrose 3%, MgSO
47H
2O 0.05%, pH 6.7, time: 48-56 hour;
The same Penicillium decumbens of Rhizopus oryzae slant medium:
Rhizopus oryzae fermention medium: Vogel ' s mother liquor 4%, hemicellulose 1%, trace element 0.01%, temperature: 28-30, time: 2 days, micro-component: 2NHCl 5ml, FeSO
40.25g, MnSO
4H
2O 0.98g, ZnCl
20.83g, CoCl
21.0g, aquae destillata 100ml;
Aspergillus niger slant medium: NaNO
30.3%, KCl 0.05%, K
2HPO
40.1%, FeSO
40.001%, sucrose 3.0%, MgSO
47H
2O 0.05%, agar 2.0%, pH 6.7, time: 28-32 hour;
Fermention medium: Vogel ' s mother liquor 4%, Microcrystalline Cellulose 1%, trace element 0.01%, micro-component: 2NHCl 5ml, FeSO
40.25g, MnSO
4H
2O 0.98g, ZnCl
20.83g, CoCl
21.0g, aquae destillata 100ml, its same Rhizopus oryzae of filling a prescription;
(4) shake pipe: get an articulating and go into sterilization and be equipped with in the 10ml liquid nutrient medium test tube from long good slant strains, shaking table was cultivated 1-2 days under 25-38 ℃ of condition, except that Lactobacterium acidophilum, the static cultivation of propionibacterium freudenreichii;
(5) triangular flask is cultivated: pack into after the fermention medium 100-120ml sterilization with the 500ml triangular flask, pour in the triangular flask the good test tube that shakes training into 25-38 ℃ under spirit lamp and shake and trained 1-2 days;
(6) go up a jar enlarged culturing: after the fermention medium preparation, autoclaving 0.1MPa, time: 20-50 minute, be cooled to 30-38 ℃ and use aseptic technique, cultured triangular flask bacterial classification is inserted in the jar, inoculum size is 2-10%, under 25-38 ℃ of condition, ventilate or stuffy cultivation, ventilate and cultivate 8-12m
3/ h, tank pressure 0.01-0.08Mpa, incubation time 18-24 hour, stuffy need stirred once every 4-5 hour.
The proportioning of each bacterial classification: with cultured bacterium by 1: 1: 1: 1: 1: 1: fit in the fermentation using bacteria agent at 1: 1, cultured yeast is pressed 1: 1: 0.5 synthetic yeast starter, cultured mould was made the mold fermentation agent by 1: 1: 1, three kinds of starters were pressed 0.5: 1: 1; 0.5: 2: 1; 0.5: 3: 1; 1: 0.5: 1; 1: 1: 1; 1: 2: 1; 1: 3: 1; 2: 0.5: 1; 2: 1: 1; Be made into the starter of different ratio bacterial classifications at 2: 3: 1 as the concentrated feed fermentation.
Embodiment one: produce the making method of many bacterial classifications of 1000kg microbial starter culture and the application in cow concentrated feed thereof: farm crop corn 410Kg, certain herbaceous plants with big flowers cake 160Kg, calabash fiber crops cake 135Kg, soya-bean cake 130Kg, wheat bran 70Kg, straw powder 50Kg, mineral substance 40Kg (being comprised: CaCO
340-60%, CaHPO
4.2H
2O15-25%, NaCl20-30%, MgSO
4.7H
2O0.3-0.8%, Se20-70PPm, I1000-150PPm, Co20-30PPm, Mn0.05-0.15%), after the raw material pulverizing such as urea 5Kg, after 20 orders sieve, amount of water is that 100Kg added behind the water material moistening 1 hour, the raw material that profit is good adds the starter that 100L mixes by 1000Kg, the blending ratio of raw material and water is 1: 0.5-1, mix back pack fermentation, every packed 20-40kg, fermentation time: can decide according to bacterial classification, as using spontaneous fermentation 4-7 in summer days, in sky 12-17 days spring and autumn, in 1-2 month winter, be finished product after the fermentation.
The finished product proterties: color golden yellow or raw material primary colors have sour fragrance quality softness;
Concentrated feed fermentation back: crude protein 18-22%, robust fibre 6-12%, ash content 7-8%, moisture 45-50%, phosphorus 0.7-0.9%, calcium 1.15-1.30%;
Various enzyme content: cellulase: 98-105% unit/g, hemicellulase 18-21 unit/g, polygalacturonase 50-93 unit/g, proteinase 8 50-950 unit/g, dextran glycosides enzyme 60-70 unit/g.
Embodiment two: produce the making method of many bacterial classifications of 1000kg microbial starter culture and the application in cow concentrated feed thereof: farm crop corn 500Kg, certain herbaceous plants with big flowers cake 170Kg, calabash fiber crops cake 100Kg, soya-bean cake 100Kg, wheat bran 50Kg, straw powder 40Kg, mineral substance 32Kg (being comprised: CaCO
340-60%, CaHPO
4.2H
2O15-25%, NaCl20-30%, MgSO
4.7H
2O0.3-0.8%, Se 20-70PPm, I 100-150PPm, Co20-30PPm, Mn0.05-0.15%), after the raw material pulverizing such as urea 8Kg, after 20 orders sieve, amount of water is that 100Kg added behind the water material moistening 1 hour, the raw material that profit is good adds the starter that 100L mixes by 1000Kg, the blending ratio of raw material and water is 1: 0.5-1, mix back pack fermentation, every packed 20-40kg, fermentation time: can decide according to bacterial classification, as using spontaneous fermentation 4-7 in summer days, in sky 12-17 days spring and autumn, in 1-2 month winter, be finished product after the fermentation.
Embodiment three: produce the making method of many bacterial classifications of 1000kg microbial starter culture and the application in cow concentrated feed thereof: farm crop corn 550Kg, certain herbaceous plants with big flowers cake 180Kg, calabash fiber crops cake 60Kg, soya-bean cake 73Kg, wheat bran 60Kg, straw powder 40Kg, mineral substance 30Kg (being comprised: CaCO
340-60%, CaHPO
4.2H
2O 15-25%, NaCl 20-30%, MgSO
4.7H
2O 0.3-0.8%, Se 20-70PPm, I1000-150PPm, Co20-30PPm, Mn0.05-0.15%), after the urea 3Kg raw material pulverizing, after 20 orders sieve, amount of water is that 100Kg added behind the water material moistening 1 hour, the raw material that profit is good adds the starter that 100L mixes by 1000Kg, the blending ratio of raw material and water is 1: 0.5-1, mix back pack fermentation, every packed 20-40kg, fermentation time: decide according to bacterial classification, spontaneous fermentation 4-7 in summer days, in sky 12-17 days spring and autumn, in 1-2 month winter, be finished product after the fermentation.
Claims (3)
1, a kind of making method of many bacterial classifications microbial starter culture is characterized in that: the making step of starter is as follows:
Spawn culture: (1) former slant strains: 1) 8 kinds of bacteriums: dinitrogen cellulomonas cartae (Cellulomonasbiazotea); Produce yellowish fiber Zymomonas mobilis (Cellulomonas flavigena); Subtilis 10264 (Bacillus subtilis); Bacillus coagulans (Bacillus coagulans); Lactobacterium acidophilum (Lactobacillus acidophilus); Starch milk bacillus (Lactobacillus amylopHllus); Propionibacterium freudenreichii (propionibacterium freudenreichii), subtilis TQ13055 (Bacillus subtilis);
2) 3 primary yeast bacterium: Candida utilis (Candida utilis); Saccharomyces diastaticus (Saccharomyces diastaticus); Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae);
3) 3 kinds of moulds: Penicillium decumbens (Penicillium decumbens); Rhizopus oryzae (Rhizopusoryzae); Aspergillus niger (Aspergillus niger);
(2) purebred separation: get 1 ring from former slant strains and put into and killed bacterium and granulated glass sphere was housed and the triangular flask of 100ml water shakes up, with aseptic technique, get the plate that 1ml puts into sterilization and substratum is housed with aseptic straw, with aseptic glass slicker with bacterium liquid drawout, and with this scraper scrape second, third continuously, the 4th, the 5th identical plate is different dilutions and separates plate, put into 25-38 ℃ of insulation can and cultivate, the single bacterium colony that grows in the plate is inserted slant medium continue to put into after cultivation grows up to 4 ℃ of refrigerators preservations;
(3) liquid and slant culture: the dinitrogen cellulomonas cartae, produce the yellowish fiber Zymomonas mobilis, with straw powder substratum: straw 3-5%, boil added in 10 minutes yeast powder a little, natural pH;
Subtilis 10264, subtilis TQ13055, beef-protein medium: extractum carnis 0.5%, peptone 1%, sodium-chlor 0.5%, pH7.2-7.6, not adding agar is liquid nutrient medium, adding 1.5-2% agar is slant medium, use after 125 ℃ of sterilizations, the rest may be inferred;
Bacillus coagulans: yeast powder 0.8%, peptone 1%, glucose 0.7%, potassium primary phosphate 0.18%, sal epsom 0.11%, pH 7.0-7.2;
Propionibacterium freudenreichii: yeast powder 0.8%, peptone 1%, sucrose 1.5%, ammonium phosphate 0.4-0.6%, pH 6.8-7.0;
Lactobacterium acidophilum: skim-milk 10%, sugar 5%, natural pH, temperature: 38-39 ℃, cultivated 4-8 hour;
Starch milk bacillus: yeast extract paste 1%, peptone 1.0%, (NH
4)
2SO
40.5%, sucrose 2.0%, pH 7.2-7.4;
Fermention medium: dinitrogen cellulomonas cartae, product yellowish fiber Zymomonas mobilis: grass meal 2%, wheat bran 0.5%, yeast powder 0.5%; PH 7.2-7.4, temperature: 28-30 ℃, cultivated 20-22 hour;
Subtilis 10264 TQ13055: beef-protein medium: extractum carnis 0.5%, peptone 1%, sodium-chlor 0.5%, glucose 0.2%, pH 7.2-7.6; Temperature: 25-30 ℃, time: 22-24 hour;
Bacillus coagulans: yeast extract paste 1.0%, peptone 1.0%, glucose 0.6%, potassium primary phosphate 0.2%, manganous sulfate 0.1%, sal epsom 0.11%, pH 7.0; Temperature: 25-30 ℃, time 22-24 hour;
Propionibacterium freudenreichii: corn steep liquor 0.5%, glucose 0.2%, ammonium phosphate 0.02%, K
2HPO
40.08%, getting concentration is 0.1% CoCl
20.04ml, aquae destillata 100ml, pH 6.8-7.0, temperature: 25-30, time: 24-28 hour;
Lactobacterium acidophilum: peptone 1.0%, extractum carnis 1.0%, yeast extract paste 0.5%, glucose 2%, K
2HPO
40.2%, sodium-acetate 0.5%, disodium citrate 0.2%, MgSO
47H
2O 0.2%, MnSO
44H
2O 0.1%, milk powder 1%, tween 80 .1%, pH 6.0-6.5, temperature: 38-39 ℃, cultivated 4-8 hour;
Starch milk bacillus: Zulkovsky starch 2.0%, MgSO
47H
2O 0.05%, KNO
30.1%, FeSO
40.001%, NaCl 0.05%, K
2HPO
40.05%, 7.2-7.4, temperature: 25-30 ℃, time: 26-28 hour;
Yeast: Candida utilis, saccharomyces diastaticus slant medium: peptone 1.0%, yeast powder 0.5%, glucose 2.0%, pH 6.5 or natural pH;
The yeast saccharomyces cerevisiae slant medium: 5% corn hydrolyzed solution adds MgSO
47H
2O 0.3%, K
2HPO
40.15%, CaCO
30.5%, yeast powder 0.5%, pH 5-6, time: 24 hours;
Mould: Penicillium decumbens slant medium: potato 20%, sucrose 2%, K
2HPO
40.3%, MgSO
47H
2O0.15%, agar 2.0%, pH 6.0;
Fermention medium: NaNO
30.3%, KCl 0.05%, K
2HPO
40.1%, FeSO
40.001%, sucrose 3%, MgSO
47H
2O 0.05%, pH 6.7, time: 48-56 hour;
The same Penicillium decumbens of Rhizopus oryzae slant medium:
Rhizopus oryzae fermention medium: Vogel ' s mother liquor 4%, hemicellulose 1%, trace element 0.01%, temperature: 28-30, time: 2 days, micro-component: 2NHCl 5ml, FeSO
40.25g, MnSO
4H
2O 0.98g, ZnCl
20.83g, CoCl
21.0g, aquae destillata 100ml;
Aspergillus niger slant medium: NaNO
30.3%, KCl 0.05%, K
2HPO
40.1%, FeSO
40.001%, sucrose 3.0%, MgSO
47H
2O 0.05%, agar 2.0%, pH 6.7, time: 28-32 hour;
Fermention medium: Vogel ' s mother liquor 4%, Microcrystalline Cellulose 1%, trace element 0.01%, micro-component: 2NHCl 5ml, FeSO
40.25g, MnSO
4H
2O 0.98g, ZnCl
20.83g, CoCl
21.0g, aquae destillata 100ml, its same Rhizopus oryzae of filling a prescription;
(4) shake pipe: get an articulating and go into sterilization and be equipped with in the 10ml liquid nutrient medium test tube from long good slant strains, shaking table was cultivated 1-2 days under 25-38 ℃ of condition, except that Lactobacterium acidophilum, the static cultivation of propionibacterium freudenreichii;
(5) triangular flask is cultivated: pack into after the fermention medium 100-120ml sterilization with the 500ml triangular flask, pour in the triangular flask the good test tube that shakes training into 25-38 ℃ under spirit lamp and shake and trained 1-2 days;
(6) go up a jar enlarged culturing: after the fermention medium preparation, autoclaving 0.1MPa, time: 20-50 minute, be cooled to 30-38 ℃ and use aseptic technique, cultured triangular flask bacterial classification is inserted in the jar, inoculum size is 2-10%, under 25-38 ℃ of condition, ventilate or stuffy cultivation, ventilate and cultivate 8-12m
3/ h, tank pressure 0.01-0.08Mpa, incubation time 18-24 hour, stuffy need stirred once every 4-5 hour.
2, the making method of a kind of many bacterial classifications microbial starter culture according to claim 1, it is characterized in that: the proportioning of each bacterial classification: with cultured bacterium by 1: 1: 1: 1: 1: 1: fit in the fermentation using bacteria agent at 1: 1, cultured yeast is pressed 1: 1: 0.5 synthetic yeast starter, cultured mould was made the mold fermentation agent by 1: 1: 1, three kinds of starters were pressed 0.5: 1: 1; 0.5: 2: 1; 0.5: 3: 1; 1: 0.5: 1; 1: 1: 1; 1: 2: 1; 1: 3: 1; 2: 0.5: 1; 2: 1: 1; Be made into the starter of different ratio bacterial classifications at 2: 3: 1 as the concentrated feed fermentation.
3, the making method of a kind of many bacterial classifications microbial starter culture according to claim 1 and 2, it is characterized in that: with farm crop corn 400-600Kg, certain herbaceous plants with big flowers cake 150-200Kg, calabash fiber crops cake 50-150Kg, soya-bean cake 50-150Kg, wheat bran 30-80Kg, straw powder 30-50Kg, mineral substance 20-50Kg, after the urea 2.5-10Kg raw material pulverizing, after 20 orders sieve, cooperation is mixed thoroughly, amount of water is 40-50%, added behind the water material moistening 1 hour, the raw material that profit is good adds the starter that 100L mixes by 1000Kg, the blending ratio of raw material and water is 1: 0.5-1, mix back pack fermentation, every packed 20-40kg, fermentation time: decide spontaneous fermentation 4-7 in summer days, sky 12-17 days spring and autumn according to bacterial classification, in 1-2 month winter, be finished product after the fermentation.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4933364A (en) * | 1979-07-11 | 1990-06-12 | International Minerals & Chemical Corp. | Process for promoting growth and feed efficiency of food producing mammals |
| CN1117803A (en) * | 1994-08-29 | 1996-03-06 | 黄殿鹏 | Novel feed (probiotics) and production process thereof |
| CN1139520A (en) * | 1996-03-05 | 1997-01-08 | 高银相 | Production method for biological multi-bacterial fermented fodder made from stalks |
| CN1193470A (en) * | 1997-03-19 | 1998-09-23 | 天门市专利技术实施公司 | Yeast for producing fodder from stalks and preparation thereof |
| CN1425317A (en) * | 2003-01-20 | 2003-06-25 | 丁友昉 | Multiple microorganism straw fermented fodder and leaven and its preparing method |
| CN1435113A (en) * | 2003-03-06 | 2003-08-13 | 天津科技大学 | Process for producing stalk protein feed by multi-culture united anaerobic fermentation |
-
2004
- 2004-04-27 CN CNB2004100376139A patent/CN1324129C/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4933364A (en) * | 1979-07-11 | 1990-06-12 | International Minerals & Chemical Corp. | Process for promoting growth and feed efficiency of food producing mammals |
| CN1117803A (en) * | 1994-08-29 | 1996-03-06 | 黄殿鹏 | Novel feed (probiotics) and production process thereof |
| CN1139520A (en) * | 1996-03-05 | 1997-01-08 | 高银相 | Production method for biological multi-bacterial fermented fodder made from stalks |
| CN1193470A (en) * | 1997-03-19 | 1998-09-23 | 天门市专利技术实施公司 | Yeast for producing fodder from stalks and preparation thereof |
| CN1425317A (en) * | 2003-01-20 | 2003-06-25 | 丁友昉 | Multiple microorganism straw fermented fodder and leaven and its preparing method |
| CN1435113A (en) * | 2003-03-06 | 2003-08-13 | 天津科技大学 | Process for producing stalk protein feed by multi-culture united anaerobic fermentation |
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