CN119613468A - Method for maintaining solution stability in separation and purification process of oligosaccharide fermentation liquor - Google Patents
Method for maintaining solution stability in separation and purification process of oligosaccharide fermentation liquor Download PDFInfo
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- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
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- C07H5/00—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium
- C07H5/04—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium to nitrogen
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Abstract
本申请涉及生物技术领域，具体公开了一种维持低聚糖发酵液分离提纯过程中溶液稳定性的方法，本申请的低聚糖发酵液在纯化分离过程中，调节低聚糖发酵液通过顺序式模拟移动床色谱系统时的pH为6～8，温度为40℃～100℃，可以减少此纯化分离过程中的低聚糖和其他组分的变化(例如岩藻糖乳糖、乳糖、葡萄糖等)，达到维持低聚糖溶液稳定性的目的，最终达到较好的分离纯化效果，获得较高纯度的产品。该方法可以应用在岩藻糖乳糖(FL)、乳糖‑N‑四糖(LNT)、乳糖‑N‑新四糖(LNnT)、乳糖‑N‑岩藻五糖(LNPFI)等其他人乳低聚糖或其他含有乳糖基的糖类物质合成过程中，具有较高的应用价值。The present application relates to the field of biotechnology, and specifically discloses a method for maintaining the stability of a solution during the separation and purification of an oligosaccharide fermentation liquid. The oligosaccharide fermentation liquid of the present application is regulated to a pH of 6 to 8 during the purification and separation process when the oligosaccharide fermentation liquid passes through a sequential simulated moving bed chromatography system, and the temperature is 40°C to 100°C, and the changes in oligosaccharides and other components in this purification and separation process (such as fucose lactose, lactose, glucose, etc.) can be reduced, and the purpose of maintaining the stability of the oligosaccharide solution is achieved, and a better separation and purification effect is finally achieved, and a product of higher purity is obtained. The method can be applied to other human milk oligosaccharides such as fucose lactose (FL), lactose-N-tetraose (LNT), lactose-N-neotetraose (LNnT), lactose-N-fucopentaose (LNPFI), or other lactose-containing sugar substances in the synthesis process, and has a high application value.
Description
Technical Field
The application relates to the technical field of biology, in particular to a method for maintaining solution stability in the process of separating and purifying oligosaccharide fermentation liquor.
Background
The human milk oligosaccharide (hereinafter referred to as oligosaccharide) is the third largest solid component in human milk after lactose and fat, and the content is about 5 to 15g/L. The oligosaccharide comprises 5 monomers, namely D-glucose, D-galactose, N-acylglucosamine, L-fucose and N-acetylneuraminic acid (sialic acid), and thousands of oligosaccharides can be formed by combining the above 5 monomers according to different proportions, and about 200 types of oligosaccharides are clearly identified to be successful. Common oligosaccharides include 2'-fucosyllactose (2' -fucosyllactose,2 '-FL), 3-fucosyllactose (3-fucosyllactose, 3-FL), and 6' -sialyllactose (6 '-sialyllactose,6' -SL). The reducing end of the oligosaccharide is a lactose structure, and the oligosaccharide chain is extended to form oligosaccharide with various structures, and the oligosaccharide can be mainly divided into three groups according to the connected monomers, namely (1) fucosylated neutral oligosaccharide, (2) sialylated acid oligosaccharide and (3) nonfucosylated neutral oligosaccharide.
At present, the oligosaccharide is mainly prepared by microbial fermentation, the separation and purification process of the oligosaccharide fermentation liquor mainly comprises the following steps of 1) carrying out solid-liquid separation on the oligosaccharide fermentation liquor to remove thalli, then heating, adding activated carbon for adsorption and filtration, decoloring and deproteinizing, and then carrying out ion exchange desalination to obtain the primarily purified oligosaccharide clear liquor, 2) further purifying, wherein the obtained clear liquor contains other impurities such as lactose, galactose, glucose, isomers and the like besides the oligosaccharide target substances, and the separation of the saccharide substances is difficult, so that the separation of the saccharide substances can be carried out by adopting continuous simulated moving bed chromatography (SSMB) to obtain purer oligosaccharide, but the simulated moving bed SSMB has certain separation conditions in the use process, because the oligosaccharide is an oligosaccharide type, the viscosity is higher, the concentration of the oligosaccharide feed liquid entering the SSMB is about 20-70% (w/w), the viscosity is higher, the material liquid temperature is generally required to be raised when the SSMB is used for maintaining good fluidity and separation performance, the temperature is generally selected to be 40-60 ℃, and the temperature is low, and the economic and the use is not easy. In addition, the pH of the initial solution of the oligosaccharide is generally about 5, and after entering the SSMB, the pH of the solution is generally less than 5 because the resin in the SSMB contains a part of hydrogen-type resin, so that the solution is very unfavorable for the stability of the oligosaccharide, and particularly, the solution is easily degraded or decomposed into other small molecular substances or changed into other large molecular substances under the condition of 40-60 ℃.
Disclosure of Invention
The application aims to overcome the defects of the prior art and provide a method for maintaining the solution stability in the process of separating and purifying the oligosaccharide fermentation liquor, thereby achieving better separation effect and obtaining a product with higher purity. The method provided by the application solves the problem of stability of human milk oligosaccharide solution in separation and purification processes including but not limited to SSMB process and including but not limited to the condition of 40-60 ℃.
In order to achieve the above purpose, the technical scheme adopted by the application is as follows:
the application provides a method for maintaining solution stability in the process of separating and purifying oligosaccharide fermentation liquor, which comprises the following steps:
Sequentially performing degerming, decolorizing, electrodialysis desalination, ion exchange resin desalination, concentration, sequential simulated moving bed chromatographic system purification, ultrafiltration, re-concentration and spray drying on the oligosaccharide fermentation liquor;
The pH value of the oligosaccharide fermentation liquor after degerming, decoloring, electrodialysis desalination, ion exchange resin desalination and concentration is regulated to be 6-8 when the oligosaccharide fermentation liquor passes through a sequential simulated moving bed chromatographic system, and the temperature is 40-100 ℃.
The inventor of the application finds through a great deal of researches and experiments that in the purification and separation process of the oligosaccharide solution, the pH value of the oligosaccharide fermentation liquor after degerming, decoloring, electrodialysis desalination, ion exchange resin desalination and concentration is regulated to be 6-8 when the oligosaccharide fermentation liquor passes through a sequential simulated moving bed chromatographic system, the temperature is 40-100 ℃, the change of oligosaccharide and other components in the purification and separation process can be reduced, and finally the purpose of maintaining the stability of the oligosaccharide solution at a certain temperature is achieved.
In some specific embodiments, the ratio of lactose, fucose, glucose and the like in the purification and separation process of the oligosaccharide solution (such as fucose lactose solution) adopting the conditions cannot be changed obviously, which indicates that the technical effect of maintaining the stability of the oligosaccharide solution can be achieved by adopting the technical scheme of the application.
As a preferred implementation mode of the method for maintaining the solution stability in the separation and purification process of the oligosaccharide fermentation liquor, the pH value of the oligosaccharide fermentation liquor after degerming, decoloring, electrodialysis desalination, ion exchange resin desalination and concentration is regulated to be 6-8, and the temperature is 40-60 ℃ when the oligosaccharide fermentation liquor passes through a sequential simulated moving bed chromatographic system.
Preferably, the pH of the oligosaccharide fermentation broth after sterilization, decolorization, electrodialysis desalination, ion exchange resin desalination and concentration is adjusted to 6 and the temperature is 40 ℃ when the oligosaccharide fermentation broth passes through a sequential simulated moving bed chromatography system.
In the technical scheme of the application, when the oligosaccharide fermentation liquor is regulated to pass through the sequential simulated moving bed chromatographic system, the preferable conditions are adopted, so that the change of oligosaccharide and other components can be reduced better, and the stability of the oligosaccharide solution is improved better.
As a preferred embodiment of the method for maintaining the solution stability in the separation and purification process of the oligosaccharide fermentation liquor, the pH of the oligosaccharide fermentation liquor after sterilization, decolorization, electrodialysis desalination, ion exchange resin desalination and concentration is regulated by adopting an alkali solution and passes through a sequential simulated moving bed chromatographic system.
As a preferred embodiment of the method for maintaining solution stability during separation and purification of an oligosaccharide fermentation broth according to the application, the alkaline solution includes, but is not limited to, at least one of sodium hydroxide, potassium phosphate, and potassium dihydrogen phosphate.
As a preferred implementation mode of the method for maintaining the solution stability in the separation and purification process of the oligosaccharide fermentation liquor, the temperature of the oligosaccharide fermentation liquor after degerming, decoloring, electrodialysis desalination, ion exchange resin desalination and concentration is adjusted to be 24-168 hours when the oligosaccharide fermentation liquor passes through a sequential simulated moving bed chromatographic system. The oligosaccharide fermentation liquor is subjected to heat preservation in a water bath kettle, and is fed while being subjected to heat preservation, so as to achieve a better separation effect.
As a preferred embodiment of the method for maintaining the solution stability in the separation and purification process of the oligosaccharide fermentation liquor, the purification conditions of the sequential simulated moving bed chromatographic system comprise:
The operation time of A, B, C, D four steps is manually set on the operation interface of the sequential simulated moving bed chromatography system, wherein the four steps comprise 339s for A step, 169s for B step, 169s for C step and 106s for D step.
The sequential simulated moving bed chromatographic system is provided with 6 resin columns, wherein the 6 resin columns are divided into 4 areas, namely Z1 (1 column), Z2 (2 columns), Z3 (2 columns) and Z4 (1 column), the 4 areas circulate in the 6 resin columns in sequence, the inlet and outlet positions change along with the change of the four areas, the A step 6 columns are operated in series, B, C are operated simultaneously, the C step is that Z1 is fed in and out of slow components, the B step Z3 is fed in and out of fast components, and the D step Z1 is fed in and Z3 is out of fast components.
The operation time is set to achieve a good separation effect, and if the operation time is changed, lactose, fucose, glucose and lactulose isomers cannot be separated from each other, so that a purification effect cannot be achieved.
As a preferred embodiment of the method for maintaining the solution stability in the separation and purification process of the oligosaccharide fermentation liquor, the purification conditions of the sequential simulated moving bed chromatographic system further comprise:
the flow rates of the four steps of A, B, C, D are manually set on the high-pressure infusion pump, the flow rate of the circulating pump is 50mL/min, the flow rate of the volumetric pump is 50mL/min, and the flow rate of the sample pump is 25mL/min.
The sequential simulated moving bed chromatographic system is provided with 3 high-pressure infusion pumps and 6 resin columns, wherein a sample pump is used for conveying feed liquid to be separated, a positive displacement pump is used for conveying pure water, and a circulating pump is used for providing circulating power for the 6 resin columns connected in series in the step A.
The change of the flow rate and the change of the running time are both to change the feeding volume, the effect of separating feed liquid with different volumes through the resin column is different, the flow rates which can be set by different pumps are also different, and the final purpose is to achieve a better separation effect.
The application adopts the purification conditions of the sequential simulated moving bed chromatographic system, can better reduce the component change in the separation and purification process of the oligosaccharide solution and maintain the stability of the oligosaccharide solution.
As a preferred embodiment of the method for maintaining the solution stability in the separation and purification process of the oligosaccharide fermentation liquor, the sequential simulated moving bed chromatography system is provided with a water bath for heat preservation of the oligosaccharide fermentation liquor in the separation and purification process.
And (3) regulating the pH of the oligosaccharide solution to 6-8, then placing the oligosaccharide solution in a water bath kettle for heat preservation and feeding, and setting the running time of A, B, C, D steps in the running process of equipment, wherein A (339 s), B (169 s), C (169 s) and D (106 s). The flow rate of each step is manually set on a high-pressure infusion pump, a circulating pump (50 mL/min), a volumetric pump (50 mL/min) and a sample pump (25 mL/min), and when the sample pump works, an oligosaccharide solution with the pH value of 6-8 and the temperature of 40-60 ℃ is brought into a resin column equipped with the equipment to be separated, so that a target product is obtained.
As a preferred implementation mode of the method for maintaining the solution stability in the separation and purification process of the oligosaccharide fermentation liquor, the ultrafiltration condition is that the mesh number of ultrafiltration is 2000D, the membrane flux is 35L/h, the membrane area is 2.0m 2, the membrane temperature is less than 35 ℃, the membrane inlet pressure is 4.2MPa, the heat exchanger pressure is 0.4MPa, and the permeation membrane pressure is-2.3 MPa.
As a preferred implementation mode of the method for maintaining the solution stability in the separation and purification process of the oligosaccharide fermentation liquor, the spray drying condition is that the air inlet temperature is 150 ℃, the air outlet temperature is 98-100 ℃, and the feeding speed is 30rpm.
The application effectively maintains the stability of the oligosaccharide solution at a certain temperature in the separation and purification process. In some specific embodiments, the fucose lactose fermentation liquid treated by the method has no obvious change in the separation and purification process, and can maintain the stability for a long time, so that a better separation and purification effect is achieved.
Compared with the prior art, the application has the following beneficial effects:
The application provides a method for maintaining solution stability in the separation and purification process of an oligosaccharide fermentation liquid, wherein in the purification and separation process of the oligosaccharide solution, the pH value of the oligosaccharide fermentation liquid (such as a fucose lactose solution) is regulated to be 6-8 when the oligosaccharide fermentation liquid passes through a sequential simulated moving bed chromatographic system, the temperature is 40-60 ℃, the change of oligosaccharide and other components in the purification and separation process can be reduced, the purpose of maintaining the stability of the oligosaccharide solution is further achieved, and finally, a better separation and purification effect is achieved, and a product with higher purity is obtained, so that the method has higher application value.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present application, the present application will be further described with reference to the following specific examples.
In the following examples, the experimental methods used were conventional methods unless otherwise specified, the materials, reagents, etc. used were commercially available, and the component materials used in each parallel experiment were the same.
Example 1
The present embodiment provides a method for maintaining stability of a fucose lactose solution, comprising the steps of:
1) Inoculating seed solution of the strain into a fermentation tank culture medium (initial lactose mass concentration is 10g/L, initial glycerol mass concentration is 20 g/L) containing lactose and glycerol according to an inoculum size of 5%, fermenting and culturing, wherein the aeration rate is 2vvm, the rotating speed is 200r/min, the culturing temperature is 37 ℃, the pH value of the fermentation tank liquid is regulated to 6.80 by using 14% of NH4OH by volume fraction in the fermentation process, the glycerol concentration is controlled at a lower level in the fermentation process so as to improve the product accumulation amount, the substrate lactose mass concentration is controlled at about (10+/-0.5) g/L, and the dissolved oxygen of the fermentation tank is (30+/-5)%, so as to obtain fermentation liquor;
2) Taking the fucose lactose fermentation broth (the isomer accounts for 1.05% of the total sugar area ratio), sequentially carrying out degerming, decoloring, electrodialysis desalination, ion exchange resin desalination, concentration, sequential simulated moving bed chromatographic purification, ultrafiltration, re-concentration and spray drying, and detecting the change of fucose lactose components.
1. And (3) degerming, namely sequentially adding LNnT fermentation liquor discharged from a fermentation tank into a Shijie 30nm ceramic composite membrane to perform degerming, wherein the membrane flux is 20L/h, the membrane area is 1.7m 2, and the membrane temperature is less than 40 ℃.
2. Removing protein, decolorizing, concentrating the sterilized fermentation liquid, heating to 90deg.C in a heating tank, maintaining the temperature for 30min, adding 4% (Jiangsu bamboo stream plant active carbon) 305 wet carbon, maintaining the temperature, and stirring for 30min. And (3) removing active carbon from the decolorized solution through a multi-layer filter to obtain the decolorized solution.
3. And (3) electrodialysis desalination, namely adding the decolorized solution into an electrodialysis instrument for desalination, setting the voltage to 39V, and ending the electrodialysis when the liquid conductivity is reduced to below 2000 mu s/cm.
4. And (3) desalting by ion exchange resin, namely passing the solution subjected to electrodialysis desalting through an anion-cation exchange resin chromatographic column at the flow rate of 1.5BV/h, passing through cation resin and then passing through anion resin, wherein the conductivity of the collected sample solution is below 20 mu s/cm.
5. Concentrating, namely concentrating the desalted liquid after ion exchange, wherein the concentration temperature is 65 ℃, the pressure in a tank is 0.09MPa, the concentration time is not more than 3 hours each time, and the concentration is 200-300 g/L.
6. Purification by subjecting the concentrate to moving bed chromatography (SSMB) and collecting the desired components.
The SSMB chromatographic fully-called sequential simulated moving bed utilizes the difference of acting forces such as distribution, adsorption, ion exchange and the like between different solutes and stationary phases and mobile phases, when the two phases are relatively moved, each solute is balanced between the two phases for multiple times, so that each solute is mutually separated, and target substances are obtained.
The operation of the equipment is divided into A, B, C, D steps, wherein the step A and the step D are independently operated, the step B, C is operated simultaneously, the step B is used for feeding out the fast component, the step C is used for feeding in the water and discharging out the slow component, and the step D is used for feeding in the water and discharging out the fast component.
The operation time of the four steps A, B, C, D, a (339 s), B (169 s), C (169 s), D (106 s), was manually set on the operation interface.
The flow rates of the steps, the circulation pump (50 mL/min), the volumetric pump (50 mL/min) and the sample pump (25 mL/min) were manually set on the high-pressure infusion pump. All parameter settings were switched to automatic mode without error, the click system was started to run, and the concentrated oligosaccharide fermentation broth was fed at different pH, different temperature and different heating time (as shown in Table 1).
7. Ultrafiltration, namely adding the components collected after SSMB into Shijie multifunctional membrane equipment (2000D) for ultrafiltration, and collecting membrane clear liquid. The membrane flux is 35L/h, the membrane area is 1.7m 2, the membrane temperature is less than 35 ℃, the membrane inlet pressure is 4.2MPa, the heat exchanger pressure is 0.4MPa, and the permeation membrane pressure is-2.3 MPa.
8. Concentrating, namely concentrating the ultrafiltered membrane clear liquid to 200 g/L-300 g/L in batches, concentrating at 50-60 ℃ under the pressure of 0.09MPa in a tank for no more than 3 hours each time, discharging the concentrated liquid, sampling, detecting and preparing to spray drying.
9. And (3) spray drying, namely spray drying the concentrated solution, wherein the air inlet temperature is 150 ℃, the air outlet temperature is 98-100 ℃, the feeding speed is 30rpm, and the sample is collected and weighed every 40 min.
10. Detecting, namely taking a small amount of sample to perform the composition.
Detection of fucose lactose:
the instrument is an Shimadzu high performance liquid chromatograph and an ultraviolet detector;
column temperature 60 ℃;
mixing acetonitrile and 10mM ammonium formate water solution (with formic acid to adjust pH to 4.5) according to a volume ratio of 7:3;
The flow rate is 1ml/min;
Column model TSKGEL AMIDE-80 μm 4.6 mmi.d.25 cm. Detection of fucose and lactose:
the instrument is an Shimadzu high performance liquid chromatograph, a differential detector;
column temperature 60 ℃;
mobile phase 0.5mol sulfuric acid;
the flow rate is 0.5ml/min;
the column model Aminex HPX-87H Column 300*7.8mm. The test results are shown in Table 1.
TABLE 1
The results are shown in Table 1, the fucose lactose solutions with pH values of 2,4, 6, 8, 10 and 12 respectively have the ratios of 98.88%, 98.65%, 98.74%, 98.80%, 94.95% and 49.89% before heating, and the fucose lactose solution with pH values of 6-8 is heated at 40-60 ℃ for 168 hours without obvious change and generation of other miscellaneous sugar.
And fucose lactose of other pH is heated at this temperature to produce fucose, lactose and other miscellaneous sugars. In addition, after the pH of the fucose lactose solution is adjusted to 2, 4, 6, 8, 10 and 12, the fucose lactose solution is heated at 80-100 ℃ to generate fucose, lactose and other miscellaneous sugars. Although the composition changes are not obvious when the experiment is carried out under the condition of 25 ℃, the solution can deteriorate to grow bacteria under the condition, and therefore, the solution can not be kept stable under the condition. In conclusion, the stability of the fucose lactose solution can be maintained by adjusting the pH of the fucose lactose solution to 6-8 and then preserving heat at 40-60 ℃.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present application and not for limiting the scope of the present application, and although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present application may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present application.
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