CN119086404A - A microfluidic optical sensor real-time hematopoietic stem cell detection device - Google Patents

A microfluidic optical sensor real-time hematopoietic stem cell detection device Download PDF

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Publication number
CN119086404A
CN119086404A CN202411257180.1A CN202411257180A CN119086404A CN 119086404 A CN119086404 A CN 119086404A CN 202411257180 A CN202411257180 A CN 202411257180A CN 119086404 A CN119086404 A CN 119086404A
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Prior art keywords
microfluidic
optical sensor
real
hematopoietic stem
signal
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CN202411257180.1A
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夏永辉
朱国庆
黄伦辉
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Institute of Hematology and Blood Diseases Hospital of CAMS and PUMC
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Institute of Hematology and Blood Diseases Hospital of CAMS and PUMC
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Priority to CN202411257180.1A priority Critical patent/CN119086404A/en
Publication of CN119086404A publication Critical patent/CN119086404A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N15/1436—Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

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  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The invention discloses a real-time hematopoietic stem cell detection device of a microfluidic optical sensor, which comprises: the invention utilizes the microfluidic chip to conduct blood sample guidance, combines an optical detection mode of optical turbidity measurement, accelerates the detection speed and the detection precision, can monitor the concentration of hematopoietic stem cells in real time, provides instant data and supports clinical instant decision. The operation is simple and convenient, no complex equipment or long-time processing process is needed, and the method is suitable for POCT environments. By combining the microfluidic technology and the optical sensor, the detection accuracy of the cell concentration is improved, and the influence of interference factors is reduced. Compared with the flow cytometry equipment, the flow cytometry equipment has simple structure and can be miniaturized, so that the flow cytometry equipment is suitable for clinical sites or other immediate detection environments. The micro-fluidic chip and the optical sensor with low energy consumption are used, so that the influence on the environment is reduced.

Description

Real-time hematopoietic stem cell detection device of micro-fluidic optical sensor
Technical Field
The invention relates to the technical field of medical equipment, in particular to a real-time hematopoietic stem cell detection device of a microfluidic optical sensor.
Background
The detection of hematopoietic stem cells today relies mainly on flow cytometry and optical turbidity measurements, but during long-term use, the drawbacks are prominent, mainly as follows:
Flow cytometry, a commonly used cell analysis technique, measures the light scattering and fluorescence properties of cells by passing them one by one through a laser beam in a liquid stream to obtain the number and characteristics of the cells. The method has the advantages of high precision and high flux, and is suitable for cell counting and sorting. However, flow cytometry generally requires complex equipment and long operating times, and is not suitable for rapid, on-site detection.
Optical turbidity measurement uses light scattering or transmission to estimate the concentration of cells in a sample. This method is generally simple and suitable for rapid analysis of liquid samples. However, the accuracy of this method may be affected by other components in the sample and may not accurately distinguish between different types of cells.
Aiming at the problems, a real-time hematopoietic stem cell detection device of a microfluidic optical sensor is provided.
Disclosure of Invention
The invention aims to provide a microfluidic optical sensor real-time hematopoietic stem cell detection device for solving the problems in the background technology.
In order to achieve the aim, the invention provides the technical scheme that a real-time hematopoietic stem cell detection device of a microfluidic optical sensor,
Comprises a micro-fluidic chip, an optical sensing system and a data processing unit;
The microfluidic chip is used for introducing a blood sample to be tested, guiding the sample to mix the sample with the reagent, and guiding the sample to an analysis processing position.
The optical sensor system irradiates the mixed sample at the analysis processing position of the microfluidic chip and converts the optical signal into an electric signal;
and the data processing unit processes the electric signals from the optical sensor system, judges the data of the electric signals, and displays real-time data and acquisition suggestions.
The microfluidic chip comprises a sample input port, a microfluidic channel, a mixing region and an analysis region, wherein the sample input port, the microfluidic channel, the mixing region and the analysis region are integrated together;
The sample input port is connected with the inlet of the microfluidic channel, the sample input port is used for introducing a blood sample to be tested, the blood sample to be tested is introduced into the microfluidic channel, the microfluidic channel is used for sequentially guiding the blood sample to be tested to the mixing area and the analysis area, and the mixing area is used for mixing the blood sample with the reagent.
Preferably, the reagent mixed with the sample in the mixing zone is a hematopoietic stem cell marker.
Preferably, the optical sensing system comprises a light source, a photoelectric detector and an optical lens, wherein the light source irradiates the blood sample in the analysis area, the photoelectric detector receives light scattered or transmitted by the light source irradiating cells in the blood sample, and focuses the light onto the photoelectric detector through the optical lens, and the photoelectric detector converts an optical signal into an electric signal and transmits the electric signal into the data processing unit.
Preferably, the optical sensing system further comprises a light source control system for controlling the on-off and brightness of the light source.
Preferably, the light source is a laser or an LED.
Preferably, the data processing unit comprises a signal processing unit and a display unit, the signal processing unit processes the electric signals converted by the photoelectric detector, calculates the cell concentration and judges whether the cell concentration reaches the acquisition strip, and the display unit displays real-time data and acquisition suggestions.
Preferably, the signal processing unit includes a signal processing module and a data processing module.
Preferably, the signal processing module comprises a signal amplifier and a filter, wherein the signal amplifier amplifies the electric signal converted by the photoelectric detector, performs filtering processing through the filter, and transmits the electric signal to the data processing module for processing.
Preferably, the data processing module comprises a microprocessor and a memory bank, wherein the microprocessor processes and judges the signals transmitted by the signal amplifier, displays the signals on the display unit, and stores the data in the memory bank.
Compared with the prior art, the invention has the beneficial effects that the real-time hematopoietic stem cell detection device of the microfluidic optical sensor,
And (3) real-time monitoring:
the invention utilizes the micro-fluidic chip to conduct blood sample guidance, combines an optical detection mode of optical turbidity measurement, accelerates the detection speed and the detection precision, can monitor the concentration of hematopoietic stem cells in real time, provides instant data, and supports clinical instant decision.
Compared with the prior art, the flow cytometry and other detection methods generally require longer processing time, are not suitable for real-time detection, and have the problems of larger interference and low accuracy in an optical detection mode of optical turbidity measurement.
The operation is simple:
the method has the advantages of simple operation, no need of complex equipment or long-time treatment process, and suitability for POCT environments.
In contrast, conventional test equipment is often complex to operate and requires specialized laboratory conditions and personnel training.
High accuracy:
the method has the advantages that the method combines a microfluidic technology and an optical sensor, improves the detection accuracy of the cell concentration, and reduces the influence of interference factors.
In contrast, the optical turbidity measurement in the prior art may be interfered by other components of the sample, and the microfluidic technology can effectively control the sample processing conditions.
Portability:
Compared with the flow cytometry equipment, the flow cytometry equipment has a simple structure and can be miniaturized, so that the flow cytometry equipment is suitable for clinical sites or other immediate detection environments.
Environmental protection:
the micro-fluidic chip and the optical sensor with low energy consumption are used, so that the influence on the environment is reduced.
In contrast, conventional detection devices may require more chemical reagents and resources, increasing environmental burden.
Drawings
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a schematic diagram of the overall structure of the present invention;
FIG. 3 is a schematic diagram of a microfluidic chip according to the present invention;
FIG. 4 is a schematic diagram of an optical sensing system according to the present invention;
FIG. 5 is a schematic diagram of a data processing unit according to the present invention.
In the figure, a microfluidic chip 1, a sample input port 11, a microfluidic channel 12, a mixing region 13, an analysis region 14, an optical sensing system 2, a light source 21, a photodetector 22, an optical lens 23, a data processing unit 3, a signal processing unit 31, a signal processing module 311, a signal amplifier 311-1, a filter 311-2, a data processing module 312, a microprocessor 312-1 and a memory bank 312-2 display unit 32 are shown.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-5, the present invention provides a technical scheme of a real-time hematopoietic stem cell detection device of a microfluidic optical sensor,
Comprises a micro-fluidic chip 1, an optical sensing system 2 and a data processing unit 3;
The microfluidic chip 1 is used for introducing a blood sample to be tested, guiding the sample to mix the sample with a reagent, and guiding the sample to an analysis processing position.
The optical sensing system 2 irradiates the mixed sample at the analysis processing position of the microfluidic chip 1 and converts the optical signal into an electric signal;
the data processing unit 3 processes the electrical signals from the optical sensor system 2, judges the data thereof, and displays real-time data and acquisition advice.
Sample processing, namely mixing a blood sample with a reagent to ensure uniformity.
And (3) real-time monitoring:
the invention uses the micro-fluidic chip 1 to conduct blood sample guidance, combines an optical detection mode of optical turbidity measurement, accelerates the detection speed and the detection precision, can monitor the concentration of hematopoietic stem cells in real time, provides instant data, and supports clinical instant decision.
Compared with the prior art, the flow cytometry and other detection methods generally require longer processing time, are not suitable for real-time detection, and have the problems of larger interference and low accuracy in an optical detection mode of optical turbidity measurement.
The operation is simple:
The invention has the advantages that the blood sample is only needed to be input from the sample input port 11, the operation is simple and convenient, no complex equipment or long-time processing process is needed, and the invention is suitable for POCT environment.
In contrast, conventional test equipment is often complex to operate and requires specialized laboratory conditions and personnel training.
High accuracy:
the method has the advantages that the microfluidic chip 1 and the optical sensing system 2 are matched by combining a microfluidic technology and an optical sensor, so that the accuracy of detecting the cell concentration is improved, and the influence of interference factors is reduced.
In contrast, the optical turbidity measurement in the prior art may be interfered by other components of the sample, and the microfluidic technology can effectively control the sample processing conditions.
Portability:
Compared with the flow cytometry equipment, the flow cytometry equipment has a simple structure and can be miniaturized, so that the flow cytometry equipment is suitable for clinical sites or other immediate detection environments.
Environmental protection:
the micro-fluidic chip and the optical sensor with low energy consumption are used, so that the influence on the environment is reduced.
In contrast, conventional detection devices may require more chemical reagents and resources, increasing environmental burden.
Specifically, the microfluidic chip 1 includes a sample input port 11, a microfluidic channel 12, a mixing region 13, and an analysis region 14, and the sample input port 11, the microfluidic channel 12, the mixing region 13, and the analysis region 14 are integrated together;
The sample input port 11 is connected with the inlet of the microfluidic channel 12, the sample input port 11 is used for introducing a blood sample to be tested, the blood sample to be tested is introduced into the microfluidic channel 12, the microfluidic channel 12 is used for sequentially introducing the blood sample to be tested into the mixing zone 13 and the analysis zone 14, and the mixing zone 13 is used for mixing the blood sample with the reagent.
Sample input port 11 through which sample is introduced, the inlet design ensures that sample smoothly enters the microfluidic channel 12.
The microfluidic channel 12 comprises a staggered flow area and a sharp turning area inside the channel, so that the sample processing effect is enhanced.
And the mixing area 13 is used for ensuring that the sample and the reagent are fully mixed and improving the detection precision.
The analysis area 14 is used for irradiating the sample by the light source 21, receiving and analyzing the light signal by the photoelectric detector to obtain the cell concentration data.
Specifically, the reagent mixed with the sample in the mixing region 13 is a hematopoietic stem cell marker.
Specifically, the optical sensing system 2 includes a light source 21, a photodetector 22, and an optical lens 23, the light source 21 irradiates the blood sample in the analysis region, the photodetector 22 receives scattered or transmitted light of the light source 21 irradiated on the cells in the blood sample, and focuses the scattered or transmitted light onto the photodetector 22 through the optical lens, and the photodetector 22 converts the optical signal into an electrical signal and transmits the electrical signal into the data processing unit 3.
The light source 21 is used to illuminate the sample flowing through the analysis zone. The light source type can be laser or LED, and a proper light source is selected to meet the detection requirement.
A light source 21, which focuses the light beam through an optical lens, ensures that the light of the light source impinges on each part of the analysis zone.
Photodetector 22 receives the scattered or transmitted light signal of the sample after it has been illuminated by the light source. The position of the photodetector 22 is opposite the light source, ensuring accurate acquisition of the signal.
The photodetector 22 collects the optical signal passing through the sample and converts the optical signal into an electrical signal for analysis by the data processing unit 3.
Specifically, the optical sensing system 2 further includes a light source control system that controls the switching and luminance of the light source 21.
Specifically, the light source 21 is a laser or an LED.
Specifically, the data processing unit 3 includes a signal processing unit 31 and a display unit 32, the signal processing unit 31 processes the electric signal converted by the photodetector 22, calculates the cell concentration and judges whether or not the acquisition bar is reached, and real-time data and acquisition advice are displayed by the display unit 32.
Specifically, the signal processing unit 31 includes a signal processing module 311 and a data processing module 312.
Specifically, the signal processing module 311 includes a signal amplifier 311-1 and a filter 311-2, where the signal amplifier 311-1 amplifies the electrical signal converted by the photodetector, performs filtering processing by the filter, and transmits the filtered electrical signal into the data processing module 312 for data determination and processing.
Specifically, the data processing module 312 includes a microprocessor 312-1 and a memory bank 312-2, and the microprocessor 312-1 processes and determines the signal input from the signal amplifier 311-1 and displays the signal on the display unit 32, and stores the data in the memory bank 312-2.
The data processing module 312 amplifies and filters the electrical signal from the photodetector 22 to improve signal quality.
The data processing module 312 calculates the cell concentration from the processed signal and compares it with a preset collection criterion.
The display unit 32 displays the cell concentration data and whether the acquisition condition is met in real time, so that the user can make a decision in real time.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. A microfluidic optical sensor real-time hematopoietic stem cell detection device,
Comprises a micro-fluidic chip (1), an optical sensing system (2) and a data processing unit (3);
The microfluidic chip (1) is used for introducing a blood sample to be tested, guiding the sample to mix the sample with the reagent and guiding the sample to an analysis processing position;
The optical sensing system (2) irradiates a mixed sample at an analysis processing position of the microfluidic chip (1) and converts an optical signal into an electric signal;
The data processing unit (3) processes the electric signals from the optical sensor system (2), judges the data thereof, and displays real-time data and acquisition suggestions.
2. The microfluidic optical sensor real-time hematopoietic stem cell detection device according to claim 1, wherein the microfluidic chip (1) comprises a sample input port (11), a microfluidic channel (12), a mixing region (13) and an analysis region (14), and the sample input port (11), the microfluidic channel (12), the mixing region (13) and the analysis region (14) are integrated together;
The sample input port (11) is connected with the inlet of the microfluidic channel (12), the sample input port (11) is used for introducing a blood sample to be tested, the blood sample to be tested is introduced into the microfluidic channel (12), the microfluidic channel (12) is used for sequentially guiding the blood sample to be tested into the mixing region (13) and the analysis region (14), and the mixing region (13) is used for mixing the blood sample with a reagent.
3. The device for real-time hematopoietic stem cell detection of microfluidic optical sensor of claim 1, wherein the reagent mixed with the sample in the mixing zone (13) is hematopoietic stem cell marker.
4. The device for real-time hematopoietic stem cell detection of microfluidic optical sensor according to claim 1, wherein the optical sensing system (2) comprises a light source (21), a photodetector (22) and an optical lens (23), wherein the light source (21) irradiates the blood sample in the analysis area, the photodetector (22) receives the scattered or transmitted light of the light source (21) irradiated on the cells in the blood sample, and focuses the scattered or transmitted light on the photodetector (22) through the optical lens, and the photodetector (22) converts the optical signal into an electrical signal and transmits the electrical signal into the data processing unit (3).
5. The device for real-time hematopoietic stem cell detection of microfluidic optical sensor according to claim 1, wherein the optical sensor system (2) further comprises a light source control system that controls the on/off and brightness of the light source (21).
6. The device for real-time hematopoietic stem cell detection of microfluidic optical sensor according to claim 1, wherein the light source (21) is a laser or an LED.
7. The device for detecting real-time hematopoietic stem cells by using a microfluidic optical sensor according to claim 1, wherein the data processing unit (3) comprises a signal processing unit (31) and a display unit (32), the signal processing unit (31) processes the electric signals converted by the photoelectric detector (22), calculates the cell concentration and judges whether the cell concentration reaches the acquisition bar, and the display unit (32) displays real-time data and acquisition advice.
8. The device for real-time hematopoietic stem cell detection of microfluidic optical sensor of claim 1, wherein the signal processing unit (31) comprises a signal processing module (311) and a data processing module (312).
9. The device for real-time hematopoietic stem cell detection of a microfluidic optical sensor according to claim 1, wherein the signal processing module (311) comprises a signal amplifier (311-1) and a filter (311-2), the signal amplifier (311-1) amplifies the electrical signal converted by the photodetector, performs filtering processing by the filter, and transmits the electrical signal into the data processing module (312) for data judgment and processing.
10. The device for real-time hematopoietic stem cell detection of a microfluidic optical sensor according to claim 1, wherein the data processing module (312) comprises a microprocessor (312-1) and a memory bank (312-2), and the microprocessor (312-1) processes and judges the signal transmitted from the signal amplifier (311-1) and displays the signal on the display unit (32) and stores the data in the memory bank (312-2).
CN202411257180.1A 2024-09-09 2024-09-09 A microfluidic optical sensor real-time hematopoietic stem cell detection device Pending CN119086404A (en)

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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202411257180.1A CN119086404A (en) 2024-09-09 2024-09-09 A microfluidic optical sensor real-time hematopoietic stem cell detection device

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121207934A (en) * 2025-11-26 2025-12-26 天津工业大学 A photonic chip for detecting multiple blood biochemical indicators

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121207934A (en) * 2025-11-26 2025-12-26 天津工业大学 A photonic chip for detecting multiple blood biochemical indicators

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