CN118388054A - Method for purifying rural black and odorous water body by using coconut husk extended ridging microbial inoculum - Google Patents

Method for purifying rural black and odorous water body by using coconut husk extended ridging microbial inoculum Download PDF

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CN118388054A
CN118388054A CN202410732487.6A CN202410732487A CN118388054A CN 118388054 A CN118388054 A CN 118388054A CN 202410732487 A CN202410732487 A CN 202410732487A CN 118388054 A CN118388054 A CN 118388054A
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black
odorous water
water body
purifying
water
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CN118388054B (en
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张媛媛
魏明俐
李双男
张海涛
寇明月
侯力沅
张洁
吕静
房吉敦
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Shandong Institute Of Petroleum And Chemical Engineering
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/007Contaminated open waterways, rivers, lakes or ponds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/05Conductivity or salinity
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/08Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2301/00General aspects of water treatment
    • C02F2301/08Multistage treatments, e.g. repetition of the same process step under different conditions
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00Specific treatment goals
    • C02F2303/02Odour removal or prevention of malodour
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00Use of specific compounds during water treatment
    • C02F2305/06Nutrients for stimulating the growth of microorganisms
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Fertilizers (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention provides a method for purifying rural black and odorous water body by using coconut husk ridging microbial inoculum, belonging to the technical field of sewage treatment. Comprising the following steps: throwing a growth promoter for activating indigenous microorganisms into a black and odorous water body; washing coco coir, desalting, compressing to dehydrate coco coir, placing coco coir in a bucket capable of bearing coco coir, taking water of black and odorous water body and bottom mud, uniformly stirring to form muddy water mixed suspension, and pouring coco coir; aerating at the bottom, placing the bottom of a water bucket by using an aeration probe for fish culture, providing sufficient oxygen for indigenous microorganisms, and domesticating; throwing in a growth promoter for activating indigenous microorganisms; and (3) placing the domesticated coconut bricks in a plastic net cage, and placing the coconut bricks at the bottom of the black and odorous water body, or extracting the black and odorous water body, and passing through the plastic net cage to obtain a functional unit.

Description

Method for purifying rural black and odorous water body by using coconut husk extended ridging microbial inoculum
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a method for purifying rural black and odorous water bodies by using a coconut husk extended ridging microbial inoculum.
Background
By the end of 2022, black and odorous water bodies in cities in the national region and above are basically eliminated, and the black and odorous water body eliminating proportion of county-level cities is only 40%.2023, 8 and 30 days, the ecological environment department issues notification about further making of environmental protection work for black and odorous water body, 7 eastern provinces of Hebei, jiangsu, zhejian, fujian, shandong, guangdong, hainan and the like are required to be basically eliminated by 2025; other provinces can steadily promote the county black and odorous water body to be remedied according to local conditions.
Compared with physical repair and chemical repair, biological (ecological) repair treatment of black and odorous water is most economical and effective. Common biological (ecological) restoration technologies are aeration reoxygenation, biological filler technology, ecological floating island technology, complex enzyme ecological purifying agent, microbial agent, indigenous microorganism amplification, biological growth promotion, biological manipulation and the like. The core of the bioremediation technology is to continuously maintain the activity of microorganisms, and for the types of microorganisms, two modes of indigenous microorganism amplification and exogenous microorganism addition exist at present. In addition, the original flora balance can be destroyed once the exogenous microorganism is introduced into the culture medium to become dominant bacteria in the later stage because the exogenous microorganism does not have natural enemies. From the viewpoint of protecting biodiversity, the amplification of the selected indigenous flora is safer and sustainable.
The microbial growth promoter contains nutrient substances, amino acids, enzyme preparations, activators or surfactants and the like required by indigenous microorganisms, can stimulate the key enzyme activity in a system, can improve the metabolic activity of the indigenous microorganisms, and promotes the microorganisms to grow rapidly in a poor environment, thereby removing pollutants in the bottom mud.
Disclosure of Invention
The invention aims to provide a method for purifying rural black and odorous water body by using coconut husk ridging microbial inoculum, which has extremely high pollution removal capacity, and after treatment, black and odorous substrate sludge is converted into brown yellow, so that the method is an in-situ treatment method and is suitable for the treatment of rural black and odorous water body.
The technical scheme of the invention is realized as follows:
the invention provides a method for purifying rural black and odorous water body by using coconut husk ridging microbial inoculum, which comprises the following steps:
(1) Adding a growth promoter for activating indigenous microorganisms into a black and odorous water body, wherein the main components comprise glucose, amino acid, compound vitamin, malan leaf juice, ganoderma lucidum residues and deionized water;
(2) Washing coco coir, desalting, compressing to dehydrate coco coir, placing coco coir in a water barrel capable of bearing coco coir, taking water and bottom mud of black and odorous water, uniformly stirring to form muddy water mixed suspension, pouring coco coir, expanding coco coir, filling muddy water mixed solution from black and odorous water, and filling muddy water mixed solution in a space between coco coir and the water barrel;
(3) Aerating at the bottom, placing the bottom of a water bucket by using an aeration probe for fish culture, providing sufficient oxygen for indigenous microorganisms, and domesticating;
(4) Throwing in a growth promoter for activating indigenous microorganisms;
(5) The domesticated coconut bricks are placed in a plastic net cage and placed at the bottom of a black and odorous water body, or the black and odorous water body is extracted and passes through the plastic net cage to obtain a functional unit, and a plurality of functional units are connected in series according to the concentration of COD of inflow water, so that the effect of improving water quality is achieved.
As a further improvement of the invention, the number of times of cleaning is 4-6.
As a further improvement of the invention, the salt content after desalination is lower than 0.4ms/cm.
As a further improvement of the invention, the mass ratio of the water of the black and odorous water body to the bottom mud is 3-5:1.
As a further improvement of the present invention, the period of acclimatization is 1 week.
As a further improvement of the invention, the domestication condition is that the aeration rate is controlled to be 0.5-1.2m 3/h in 1-2 days at the initial stage of domestication; the middle period of domestication is 4-5 days, and the aeration rate is controlled to be 0.5-2m 3/hour; the aeration rate is controlled to be 0.5-1.2m 3/h after 1-2 days at the end of acclimation.
As a further improvement of the invention, the mass of the glucose accounts for 1-1.5% of the total mass of the growth promoter, the mass of the amino acid accounts for 1-1.5% of the total mass of the growth promoter, the mass of the compound vitamin accounts for 0.005-0.01% of the total mass of the growth promoter, the mass of the juice of the Mallotus leaf blades accounts for 10-20% of the total mass of the growth promoter, and the mass of the ganoderma lucidum fungus residues accounts for 1-5% of the total mass of the growth promoter.
As a further improvement of the invention, the preparation method of the maryland leaf juice comprises the following steps: oven drying fresh herba Kalimeridis at 55-65deg.C, crushing into powder, sieving with 20-40 mesh sieve, collecting the undersize, and mixing with distilled water at a ratio of 1:3-1:4 to obtain Kalimeris indica leaf juice.
As a further improvement of the invention, the preparation method of the growth promoter comprises the steps of mixing the components in a reaction kettle, stirring for 1-3h under the condition of heating at 40-45 ℃, wherein the stirring speed is 200r/min, and regulating the pH value to 6-7.5.
As a further improvement of the invention, the growth promoter is added into the black and odorous water body according to the concentration of 5-20 ml/L.
The invention has the following beneficial effects: the invention provides a purely natural microbial inoculum and a device for efficiently activating and cultivating indigenous microorganisms, namely, nutrient substances provided for the indigenous microorganisms are nontoxic and biodegradable, and a living place provided for the indigenous microorganisms is purely natural plants. The invention has low cost, simple operation and good effect of treating black and odorous water body, and belongs to a green low-carbon energy-saving treatment technology.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The embodiment provides a method for purifying rural black and odorous water body by using coconut husk ridging microbial inoculum, which comprises the following steps:
(1) The method comprises the steps of putting a growth promoter for activating indigenous microorganisms into a black and odorous water body, wherein the main components comprise glucose, amino acid, compound vitamin, malan blade juice, ganoderma lucidum residues and deionized water, and the main components comprise:
the mass of the glucose accounts for 1-1.5% of the total mass of the growth promoting agent, and the glucose is food grade purchased in the market;
The mass of the amino acid accounts for 1-1.5% of the total mass of the growth promoter, and the amino acid is food grade purchased in the market;
the mass of the compound vitamin accounts for 0.005-0.01% of the total mass of the growth promoter, and the compound vitamin is food grade purchased in the market;
The maryland is a common weed beside rural river, has rich amino acids, vitamins and polysaccharides, and has high nutrient component content than rhizome content in the leaves; cutting fresh leaves of Malan, putting the leaves into pieces of about 2-5cm, and squeezing into juice in a portable juice squeezing cup, wherein the mass of the pure juice accounts for 2-5% of the total mass of the growth promoter; if the fresh maryland is not present beside the river in winter, the fresh maryland grass in summer can be dried in a baking oven at 60 ℃, crushed into powder, screened by a 40-mesh screen, and the undersize is taken out to prepare a mixed solution according to the proportion of the powder to distilled water of 1:3-1:4, which is equivalent to pure juice.
The ganoderma lucidum dreg is purchased from Shandong Sanxiu biotechnology limited company, the ganoderma lucidum fungus bag is a culture medium of ganoderma lucidum, after ganoderma lucidum is picked every 6 months, the rest ganoderma lucidum fungus bag is broken and sold, the ganoderma lucidum fungus dreg is called ganoderma lucidum fungus dreg, the ganoderma lucidum fungus dreg passes through a 100-mesh screen, and the screen residue is taken as the cost required by a growth promoter, and the mass ratio is 1-5%;
The rest is distilled water.
Mixing the above components in a reaction kettle, stirring for 2 hours at 40-45deg.C under heating at 200 rpm, and adjusting pH to 6-7.5.
The method for adjusting the pH value comprises the following steps: if the pH value of the growth promoter is more than 7.5, properly adding 5% ferrous sulfate solution, and regulating the pH value to be between 6 and 7.5; if the pH of the growth promoter is less than 6, 5% sodium bicarbonate is properly added, and the pH is regulated to be between 6 and 7.5.
The growth promoter is added into the domesticated sewage according to the ratio of 5 ml/L to 20 ml/L;
(2) The salt content of the coconut coir is reduced (lower than 0.4 ms/cm) after the coconut coir is washed for 5 times, and a place for providing a film for indigenous microorganisms is provided by utilizing a similar cavernous body which is formed after the coconut coir is foamed; after desalting coco coir, compressing to dehydrate coco coir, placing coco coir in a bucket capable of bearing coco coir, taking water and sediment of a black and odorous water body (cement ratio is 4:1), fully stirring to form a muddy water mixed suspension (muddy water mixed liquid for short), pouring coco coir into coco coir, expanding coco coir, filling muddy water mixed liquid from the black and odorous water body, and filling muddy water mixed liquid in a space between coco coir and the bucket;
(3) The bottom is aerated, the bottom of the water bucket is placed by an aeration probe for fish culture, sufficient oxygen is provided for indigenous microorganisms, and the aeration rate is as follows: 0.5-2m 3/hr; usually, the domestication is carried out for 1 week, and the aeration rate is controlled to be 0.5-1.2m 3/h in 1-2 days at the initial stage of domestication; the aeration rate is controlled to be 0.5-2m 3/h in the middle period of domestication for 4-5 days; the aeration rate is controlled to be 0.5-1.2m 3/h after 1-2 days at the end of domestication;
(4) Throwing in a growth promoter for activating indigenous microorganisms;
(5) The domesticated coconut bricks are placed in a plastic net cage (a functional unit) and placed at the bottom of a black and odorous water body, or the black and odorous water body is extracted, and a plurality of functional units can be connected in series through the plastic net cage according to the concentration of COD of inflow water, so that the effect of improving water quality is achieved.
Example 1
The river pit in a certain rural area is polluted by household garbage thrown by peasants and tail water in a nearby fishpond, eutrophication is serious, odor is emitted in summer, water quality is detected as 52mg/L of ammonia nitrogen, COD is 495mg/L, and total phosphorus is 4.8mg/L.
1Kg of coconut husk is foamed to 20L by using tap water, compressed and dehydrated, then foamed to 20L by using tap water, compressed and dehydrated, repeated for 5 times, and the conductivity of the washed water is 0.3ms/cm, and the coconut husk is compressed and dehydrated for standby.
Taking 18L of a mixture of river sediment and water in a ratio of 1:4, placing the mixture into a 20L water bucket, and then placing 100ml of growth promoting agent, wherein the growth promoting agent comprises: 1.5% of glucose, 1% of amino acid, 0.005% of compound vitamin, 5% of maryland juice, 2% of ganoderma lucidum dreg and the balance of distilled water. The bottom aeration is carried out for 2 days at an aeration rate of 0.5m 3/h, then the dehydrated coconut coir is put into a water bucket, the aeration is carried out for 15 days at an aeration rate of 2m 3/h, and the sewage is taken to test the water quality.
COD is 42mg/L, ammonia nitrogen is 8.1mg/L, total phosphorus is 1.6mg/L, and odor disappears.
Example 2
The excrement pit 20m 3 of the dairy farm is put into a single plastic net barrel with the volume of 20L, two plastic net barrels are respectively put into the four directions of east, south, west and north, 18L of coconut chaff is soaked in the single barrel after desalination and foaming, the dehydrated coconut chaff is soaked in a mixture 15L with the urine-excrement ratio of 4:1, and 300ml of growth promoting agent is added, wherein the growth promoting agent comprises: 1% of glucose, 1% of amino acid, 0.008% of compound vitamin, 5% of marshmallow juice, 5% of ganoderma lucidum dreg and the balance of distilled water. The aeration rate is 1m 3/h after 7 days of aeration at the bottom of the barrel.
60 Days after the putting, the malodor intensity is 55 by 203; the ammonia concentration was reduced by 68.7% from 16ppm to 5 ppm.
Example 3
The water quality of a river pit in a certain rural area is detected as 33mg/L of ammonia nitrogen, the COD is 198mg/L, and the total phosphorus is 3.0mg/L.
1Kg of coconut husk is foamed to 20L by using tap water, compressed and dehydrated, then foamed to 20L by using tap water, compressed and dehydrated, repeated for 5 times, the conductivity of the washed water is 0.3ms/cm, and the coconut husk is put into a 20L bucket after compressed and dehydrated.
Taking 18L of a mixture of river sediment and water in a ratio of 1:4, placing the mixture in a water bucket, and then placing 120ml of growth promoting agent, wherein the growth promoting agent comprises: 1% of glucose, 1% of amino acid, 0.01% of compound vitamin, 2% of marshmallow juice, 2% of ganoderma lucidum dreg and the balance of distilled water. The bottom aeration is carried out for 20 days at an aeration rate of 1.2m 3/h, and the sewage is taken to test the water quality.
COD is 40mg/L, ammonia nitrogen is 2.3mg/L, and total phosphorus is 0.6mg/L.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.

Claims (10)

1. A method for purifying black and odorous water bodies in rural areas by using coconut husk ridging microbial inoculum, which is characterized by comprising the following steps:
(1) Adding a growth promoter for activating indigenous microorganisms into a black and odorous water body, wherein the main components comprise glucose, amino acid, compound vitamin, malan leaf juice, ganoderma lucidum residues and deionized water;
(2) Washing coco coir, desalting, compressing to dehydrate coco coir, placing coco coir in a water barrel capable of bearing coco coir, taking water and bottom mud of black and odorous water, uniformly stirring to form muddy water mixed suspension, pouring coco coir, expanding coco coir, filling muddy water mixed solution from black and odorous water, and filling muddy water mixed solution in a space between coco coir and the water barrel;
(3) Aerating at the bottom, placing the bottom of a water bucket by using an aeration probe for fish culture, providing sufficient oxygen for indigenous microorganisms, and domesticating;
(4) Throwing in a growth promoter for activating indigenous microorganisms;
(5) The domesticated coconut bricks are placed in a plastic net cage and placed at the bottom of a black and odorous water body, or the black and odorous water body is extracted and passes through the plastic net cage to obtain a functional unit, and a plurality of functional units are connected in series according to the concentration of COD of inflow water, so that the effect of improving water quality is achieved.
2. The method for purifying a black and odorous water body in a rural area using a coconut husk extended culture fungicide according to claim 1, wherein the number of times of washing is 4-6.
3. The method for purifying a black and odorous water body in a rural area using a coconut husk extended culture fungicide according to claim 1, wherein the salt content after desalination is less than 0.4ms/cm.
4. The method for purifying a black and odorous water body in a rural area using a coconut husk microbial inoculum according to claim 1, wherein the mass ratio of water to sediment of the black and odorous water body is 3-5:1.
5. The method of purifying a body of black and odorous water in a rural area using a coconut coir extended cultivation soil microbial agent of claim 1, wherein the acclimation time is 1 week.
6. The method for purifying black and odorous water in rural areas by using coconut husk microbial inoculum according to claim 5, wherein the domestication condition is that the aeration rate is controlled to be 0.5-1.2m 3/hour in 1-2 days at the initial stage of domestication; the middle period of domestication is 4-5 days, and the aeration rate is controlled to be 0.5-2m 3/hour; the aeration rate is controlled to be 0.5-1.2m 3/h after 1-2 days at the end of acclimation.
7. The method for purifying rural black and odorous water body by using coconut husk spreading and ridging bacteria agent according to claim 1, wherein the mass of glucose is 1-1.5% of the total mass of the growth promoting agent, the mass of amino acid is 1-1.5% of the total mass of the growth promoting agent, the mass of compound vitamin is 0.005-0.01% of the total mass of the growth promoting agent, the mass of the juice of the leaves of the Mallotus japonicus is 10-20% of the total mass of the growth promoting agent, and the mass of ganoderma lucidum residues is 1-5% of the total mass of the growth promoting agent.
8. The method for purifying a black and odorous water body in a rural area using a coconut husk extended culture soil microbial inoculum according to claim 7, wherein the preparation method of the maryland leaf juice is as follows: oven drying fresh herba Kalimeridis at 55-65deg.C, crushing into powder, sieving with 20-40 mesh sieve, collecting the undersize, and mixing with distilled water at a ratio of 1:3-1:4 to obtain Kalimeris indica leaf juice.
9. The method for purifying rural black and odorous water body by using coconut husk ridging microbial inoculum according to claim 8, wherein the preparation method of the growth promoter is characterized in that the components are mixed in a reaction kettle, stirred for 1-3h under the condition of heating at 40-45 ℃, the stirring speed is 200r/min, and the pH is regulated to 6-7.5.
10. The method for purifying a black and odorous water body in a rural area using a coconut husk microbial inoculum according to claim 8, wherein the growth promoting agent is added to the black and odorous water body at a concentration of 5-20 ml/L.
CN202410732487.6A 2024-06-07 2024-06-07 Method for purifying rural black and odorous water body by using coconut husk extended ridging microbial inoculum Active CN118388054B (en)

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