CN116731845B - Cleaning-free biological reaction container - Google Patents

Cleaning-free biological reaction container Download PDF

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Publication number
CN116731845B
CN116731845B CN202310509343.XA CN202310509343A CN116731845B CN 116731845 B CN116731845 B CN 116731845B CN 202310509343 A CN202310509343 A CN 202310509343A CN 116731845 B CN116731845 B CN 116731845B
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shell
fixedly connected
wall
driving motor
outer side
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CN116731845A (en
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顾谷钟
郑海权
王友顺
丁涛
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Shanghai Baigaole Biotechnology Co ltd
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Shanghai Baigaole Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Accessories For Mixers (AREA)

Abstract

The invention belongs to the technical field of mixed culture, in particular to a cleaning-free biological reaction container, which comprises a mixed reaction assembly and a biological reaction bag, wherein the mixed reaction assembly comprises a reaction outer shell, a rotary inner shell, a middle wall, an inner wall, a threaded bulge, a central bulge and a first through hole, the inner side of the reaction outer shell is rotationally connected with the rotary inner shell, one end of the rotary inner shell is fixedly connected with the inner wall, the middle wall is fixedly connected in a gap between the rotary inner shell and the inner wall, one end of the inner wall is provided with the threaded bulge, the central bulge is arranged at the central position of the bottom end of the inner wall, the first through hole is formed in the inner side of one end of the inner wall, the biological inside of the biological reaction bag can be cultured and mixed by directly placing the biological reaction bag into the mixed reaction assembly, the biological reaction bag is directly taken out after the culture is completed, the mixed reaction assembly is not required to be cleaned, and meanwhile, the mixed reaction assembly has a good biological mixing function.

Description

Cleaning-free biological reaction container
Technical Field
The invention belongs to the technical field of mixed culture, and particularly relates to a cleaning-free biological reaction container.
Background
In biological mixed culture, the conventional biological culture container is polluted after the completion of the conventional biological culture, the conventional biological culture container can be reused after cleaning and sterilizing, the inner side of the conventional biological culture container is generally provided with stirring blades, the cleaning and sterilizing are troublesome, the next biological culture work can be influenced due to unqualified cleaning and sterilizing, the conventional biological culture container is generally detected after cleaning, and the workload is increased.
Disclosure of Invention
To solve the problems set forth in the background art. The invention provides a cleaning-free biological reaction container, which has the characteristics that the biological reaction bag is directly placed into the inner side of a mixed reaction assembly, so that the organisms in the inner side of the biological reaction bag can be cultured and mixed, and the biological reaction bag is directly taken out after the culture is finished, so that the mixed reaction assembly is not required to be cleaned.
In order to achieve the above purpose, the present invention provides the following technical solutions: a cleaning-free biological reaction container, a biological mixing reactor comprises a mixing reaction assembly and a biological reaction bag;
The mixing reaction assembly comprises a reaction outer shell, a rotary inner shell, a middle wall, an inner wall, a thread protrusion, a central protrusion and a first through hole, wherein the inner side of the reaction outer shell is rotationally connected with the rotary inner shell;
The biological reaction bag comprises a disposable bag body and a sealing cover, wherein the sealing cover is detachably connected to the opening at one end of the disposable bag body, and the disposable bag body can be attached to the surface of the inner wall by pumping out air between the middle wall and the inner wall.
As the cleaning-free biological reaction container, the biological reaction bag also comprises a stirring rod arranged on the inner side of the disposable bag body, wherein the stirring rod comprises a buffering flexible layer, a metal rod and a hollow part, the top end of the buffering flexible layer is provided with the hollow part, and the inner side of one end of the buffering flexible layer is fixedly connected with the metal rod.
As the cleaning-free biological reaction container, the biological mixing reactor also preferably comprises a control mixing assembly, wherein the control mixing assembly comprises a collecting shell, a control shell, a second through hole, semicircular strips, electromagnets, a dispersing shell and conductive balls, wherein the annular hollow collecting shell is arranged at the bottom of the annular hollow collecting shell, the control shell is fixedly connected with the top end of the collecting shell, the second through holes are formed in the bottom end of the control shell at equal intervals, two semicircular strips are arranged right below each second through hole, two adjacent semicircular strips can be simultaneously and electrically contacted with one conductive ball, a plurality of electromagnets are fixed on the inner wall surface of the rotary inner shell at equal intervals, and the dispersing shell is arranged on the inner side of the control shell and can enable the conductive balls to irregularly penetrate through any second through hole.
As the cleaning-free biological reaction container, the control mixing assembly also comprises a circulating track, a motor support, a third driving motor, a rotating wheel and a pushing plate, wherein the top end of the circulating track is fixedly communicated with the inner side of the central position of the top end of the control shell, the bottom end of the circulating track is fixedly communicated with the outer side of the bottom end of the collecting shell, the outer side of one end of the circulating track is fixedly connected with the motor support, the inner side of one end of the motor support is fixedly connected with the third driving motor, the tail end of a main shaft of the third driving motor is fixedly connected with the rotating wheel, the outer side of the rotating wheel is fixedly connected with a plurality of pushing plates at equal intervals, the pushing plates extend to the inner side of one end of the circulating track, and the pushing plate can push the conductive balls to move around the rotating wheel.
As the preferred cleaning-free biological reaction container, the control mixing assembly further comprises a fourth driving motor, a connecting support, a damping plate, a second bearing and a bearing support, wherein the bottom end of the fourth driving motor is fixedly connected to the central position of the inner side of the bottom end of the control shell, the connecting support is fixedly connected to the tail end of a main shaft of the fourth driving motor, one end outer side of the connecting support is fixedly connected with one end of the dispersing shell, the damping plate is fixedly connected to the upper side of the top end plane of the dispersing shell, the second bearing is fixedly connected to the outer side of the bottom of the dispersing shell, and one end of the second bearing is fixedly connected to the inner side of the bottom of the control shell through the bearing support.
As the preferred one of the washing-free biological reaction containers, the control mixing assembly further comprises a limiting ring, a third through hole, an annular support, a gear ring, a gear and a fifth driving motor, wherein the annular support is fixedly connected to the outer side of the bottom end of the control shell, the limiting ring is rotatably connected to the inner side of the annular support, the third through hole is formed in one end of the limiting ring, the gear ring is fixedly embedded into the inner side of one end of the limiting ring, one end of the fifth driving motor is fixedly connected to the outer side of one end of the collection shell, the gear is fixedly connected to the tail end of a main shaft of the fifth driving motor, and one end of the gear is in meshed connection with one end of the outer side of the gear ring.
Preferably, the mixing reaction assembly further comprises a sealing plate and a one-way valve, wherein the sealing plate is fixedly connected between the middle wall and the inner wall, the sealing plate divides the space between the middle wall and the inner wall into a plurality of independent spaces, the sealing plate does not coincide with the first through hole, and the one-way valve is fixedly communicated with the inner side of the middle wall.
As the preferred mode of the cleaning-free biological reaction container, the mixing reaction assembly further comprises an annular sleeve, a spring air pipe, a vacuum air pump, a first driving motor, a threaded rod and a threaded sleeve, wherein the outer end face of the middle wall is connected with the annular sleeve in a sliding mode, the spring air pipe is fixedly communicated with the inner side of one end of the annular sleeve, the bottom end of the spring air pipe is fixedly connected with an air suction nozzle of the vacuum air pump, one end of the vacuum air pump is fixedly connected with the outer side of the bottom end face of the rotating inner shell, the outer side of the bottom end face of the rotating inner shell is fixedly connected with the first driving motor, the tail end of a main shaft of the first driving motor is fixedly connected with the threaded rod, the threaded sleeve is connected with the outer side of one end of the threaded rod in a threaded mode, and the outer side of one end of the threaded sleeve is fixedly connected with the outer side of one end of the annular sleeve.
As the cleaning-free biological reaction container, the mixing reaction assembly also comprises a second driving motor and a first bearing, wherein the outer side of the bottom end of the second driving motor is fixedly connected with the inner side of the bottom end of the reaction outer shell, the tail end of a main shaft of the second driving motor is fixedly connected with the central position of the outer side of the bottom end of the rotating inner shell, and the rotating inner shell is rotationally connected with the inner side of the reaction outer shell through the first bearing.
As the preferred one of the cleaning-free biological reaction container, the control mixing assembly further comprises a rotating support and a reset spring, wherein the top end of the semicircular strip is rotatably connected to the inner side of one end of the collecting shell through the rotating support, the reset spring is fixedly connected between the outer end face of the bottom end of the rotating support and the inner wall face of the collecting shell, two semicircular strips which encircle to be approximately circular are obliquely arranged, and the distance between the tops of the two semicircular strips is larger than the distance between the bottoms of the two semicircular strips.
Compared with the prior art, the invention has the beneficial effects that: through the biological mixing reactor that sets up, can be at biological cultivation's work back washing-free mixed culture device, through directly putting into the biological reaction bag that sets up and mix the reaction assembly inboard, can cultivate and mix the biological reaction bag inboard organism, cultivate the back and directly take out the biological reaction bag can, need not clear up the reaction assembly that mixes, the reaction assembly that mixes has good biological mixing function simultaneously, to the microorganism that needs to strengthen the mixing, can be further through the control mixing assembly that sets up, have the washing-free function equally.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention. In the drawings:
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic structural view of a hybrid reaction assembly according to the present invention;
FIG. 3 is an enlarged schematic view of the structure of FIG. 2A according to the present invention;
FIG. 4 is an enlarged schematic view of the structure of FIG. 2B according to the present invention;
FIG. 5 is an enlarged schematic view of the structure of FIG. 2 at C in the present invention;
FIG. 6 is a schematic view of the internal structure of the check valve according to the present invention;
FIG. 7 is a schematic view showing the internal structure of the rotary inner housing according to the present invention;
FIG. 8 is a schematic diagram of a control mixing assembly according to the present invention;
FIG. 9 is an enlarged schematic view of the structure of FIG. 8D in accordance with the present invention;
FIG. 10 is an enlarged schematic view of the structure of FIG. 8E according to the present invention;
FIG. 11 is an enlarged schematic view of the structure of the present invention at F of FIG. 8;
FIG. 12 is a schematic view showing the internal structure of the control housing according to the present invention;
FIG. 13 is a top view of a second through hole according to the present invention;
FIG. 14 is a top view of a third through hole according to the present invention;
FIG. 15 is a schematic structural view of a bioreactor bag according to the present invention;
FIG. 16 is a schematic view showing the internal structure of a stirring rod according to the present invention.
In the figure:
1. A biological mixing reactor;
2. A mixing reaction assembly; 21. a reaction housing; 211. rotating the inner housing; 212. a middle wall; 213. an inner wall; 214. a threaded boss; 215. a central boss; 216. a first through hole;
22. A sealing plate; 23. a one-way valve; 231. a valve body housing; 232. a fixed ventilation board; 233. a slide bar; 234. a hard plate; 235. a seal ring; 236. a spring seat; 237. a compression spring;
24. an annular sleeve; 241. a limit rod; 242. a spring air tube; 243. a vacuum air pump; 245. a first driving motor; 246. a threaded rod; 247. a thread sleeve;
25. A second driving motor; 251. a first bearing;
3. Controlling the mixing assembly; 31. a collection housing; 311. a control housing; 312. a second through hole; 313. a semicircular strip; 314. an electromagnet; 315. a dispersion housing; 316. a conductive ball;
32. A circulation track; 321. a motor bracket; 322. a third driving motor; 323. a rotating wheel; 324. a push plate;
33. A fourth driving motor; 331. a connecting bracket; 332. a shock absorbing plate; 333. a second bearing; 334. a bearing support;
34. a sealing sheet; 341. an internal thread portion; 342. an external thread portion;
35. A restriction ring; 351. a third through hole; 352. an annular bracket; 353. a gear ring; 354. a gear; 355. a fifth driving motor;
36. rotating the bracket; 361. a return spring;
4. A biological reaction bag; 41. a disposable bag body; 42. sealing cover; 43. a stirring rod; 431. a buffer flexible layer; 432. a metal rod; 433. a hollow portion.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1-16:
the cleaning-free biological reaction container comprises a biological mixing reactor 1, a mixing reaction assembly 2 and a biological reaction bag 4;
The mixing reaction assembly 2 comprises a reaction outer shell 21, a rotation inner shell 211, a middle wall 212, an inner wall 213, a thread protrusion 214, a central protrusion 215 and a first through hole 216, wherein the inner side of the reaction outer shell 21 is rotationally connected with the rotation inner shell 211, one end of the rotation inner shell 211 is fixedly connected with the inner wall 213, the middle wall 212 is fixedly connected in a gap between the rotation inner shell 211 and the inner wall 213, one end of the inner wall 213 is provided with the thread protrusion 214, the central protrusion 215 is arranged at the central position of the bottom end of the inner wall 213, and the first through hole 216 is formed in the inner side of one end of the inner wall 213;
The bioreactor bag 4 comprises a disposable bag 41 and a sealing cover 42, wherein the sealing cover 42 is detachably connected to the opening of one end of the disposable bag 41, and the disposable bag 41 can be attached to the surface of the inner wall 213 by pumping out the air between the middle wall 212 and the inner wall 213.
Further, the method comprises the steps of;
In an alternative embodiment, the bioreactor bag 4 further comprises a stirring rod 43 arranged on the inner side of the disposable bag 41, the stirring rod 43 comprises a buffering flexible layer 431, a metal rod 432 and a hollow part 433, the hollow part 433 is arranged at the top end of the buffering flexible layer 431, and the metal rod 432 is fixedly connected to the inner side of one end of the buffering flexible layer 431.
In an alternative embodiment, the biological mixing reactor 1 further includes a control mixing assembly 3, the control mixing assembly 3 includes a collection housing 31 with a ring-shaped hollow arrangement and an inclined bottom, a control housing 311, second through holes 312, semicircular strips 313, electromagnets 314, a dispersion housing 315 and conductive balls 316, the top end of the collection housing 31 is fixedly connected with the control housing 311, the bottom end of the control housing 311 is provided with the second through holes 312 at equal intervals, two semicircular strips 313 are arranged right below each second through hole 312, two adjacent semicircular strips 313 can be simultaneously in electrical contact with one conductive ball 316, a plurality of electromagnets 314 are fixed on the inner wall 213 surface of the rotary inner housing 211 at equal intervals, the inner side of the control housing 311 is provided with the dispersion housing 315, and the dispersion housing 315 can enable the conductive balls 316 to irregularly pass through any one second through hole 312.
In an alternative embodiment, the control mixing assembly 3 further includes a circulation track 32, a motor support 321, a third driving motor 322, a rotating wheel 323 and a pushing plate 324, the top end of the circulation track 32 is fixedly connected to the inner side of the central position of the top end of the control housing 311, the bottom end of the circulation track 32 is fixedly connected to the outer side of the bottom end of the collection housing 31, the outer side of one end of the circulation track 32 is fixedly connected with the motor support 321, the inner side of one end of the motor support 321 is fixedly connected with the third driving motor 322, the tail end of a main shaft of the third driving motor 322 is fixedly connected with the rotating wheel 323, the outer side of the rotating wheel 323 is fixedly connected with a plurality of pushing plates 324 at equal intervals, the pushing plates 324 extend to the inner side of one end of the circulation track 32, and the pushing plates 324 rotate around the rotating wheel 323 to push the conductive balls 316 to move.
In an alternative embodiment, the control hybrid assembly 3 further includes a fourth driving motor 33, a connecting bracket 331, a damping plate 332, a second bearing 333, and a bearing bracket 334, where the bottom end of the fourth driving motor 33 is fixedly connected to the central position inside the bottom end of the control housing 311, the end of the spindle of the fourth driving motor 33 is fixedly connected to the connecting bracket 331, one end outer side of the connecting bracket 331 is fixedly connected to one end of the dispersing housing 315, the damping plate 332 is fixedly connected to the upper side of the top end plane of the dispersing housing 315, the second bearing 333 is fixedly connected to the outer side of the bottom of the dispersing housing 315, and one end of the second bearing 333 is fixedly connected to the inner side of the bottom of the control housing 311 through the bearing bracket 334.
In an alternative embodiment, the control mixing assembly 3 further includes a limiting ring 35, a third through hole 351, an annular bracket 352, a gear ring 353, a gear 354 and a fifth driving motor 355, the outer side of the bottom end of the control housing 311 is fixedly connected with the annular bracket 352, the inner side of the annular bracket 352 is rotatably connected with the limiting ring 35, one end of the limiting ring 35 is provided with the third through hole 351, the inner side of one end of the limiting ring 35 is fixedly embedded with the gear ring 353, one end of the fifth driving motor 355 is fixedly connected with the outer side of one end of the collecting housing 31, the end of the main shaft of the fifth driving motor 355 is fixedly connected with the gear 354, and one end outer side of the gear 354 is in meshed connection with one end outer side of the gear ring 353.
In an alternative embodiment, the mixing reaction assembly 2 further includes a sealing plate 22 and a check valve 23, wherein the sealing plate 22 is fixedly connected between the middle wall 212 and the inner wall 213, the sealing plate 22 divides the space between the middle wall 212 and the inner wall 213 into a plurality of independent spaces, the sealing plate 22 is not overlapped with the first through hole 216, and the check valve 23 is fixedly connected with the inner side of the middle wall 212.
In an alternative embodiment, the mixing reaction assembly 2 further includes an annular sleeve 24, a spring air pipe 242, a vacuum air pump 243, a first driving motor 245, a threaded rod 246 and a threaded sleeve 247, the outer end surface of the middle wall 212 is slidably connected with the annular sleeve 24, one end inner side of the annular sleeve 24 is fixedly communicated with the spring air pipe 242, the bottom end of the spring air pipe 242 is fixedly connected with an air suction nozzle of the vacuum air pump 243, one end of the vacuum air pump 243 is fixedly connected with the outer side of the bottom end surface of the rotary inner shell 211, the outer side of the bottom end surface of the rotary inner shell 211 is fixedly connected with the first driving motor 245, the tail end of a spindle of the first driving motor 245 is fixedly connected with the threaded rod 246, one end outer side of the threaded rod 246 is in threaded connection with the threaded sleeve 247, and one end outer side of the threaded sleeve 247 is fixedly connected with one end outer side of the annular sleeve 24.
In an alternative embodiment, the hybrid reaction assembly 2 further includes a second driving motor 25 and a first bearing 251, wherein the outer side of the bottom end of the second driving motor 25 is fixedly connected to the inner side of the bottom end of the reaction outer shell 21, the end of the main shaft of the second driving motor 25 is fixedly connected to the central position of the outer side of the bottom end of the rotary inner shell 211, and the rotary inner shell 211 is rotatably connected to the inner side of the reaction outer shell 21 through the first bearing 251.
In an alternative embodiment, the control mixing assembly 3 further includes a rotating bracket 36 and a return spring 361, wherein the top ends of the semicircular strips 313 are rotatably connected to the inner side of one end of the collecting housing 31 through the rotating bracket 36, the return spring 361 is fixedly connected between the outer end surface of the bottom end of the rotating bracket 36 and the inner wall 213 of the collecting housing 31, and two semicircular strips 313 enclosing an approximate circular tube shape are arranged in an inclined manner, and the interval between the tops of the two semicircular strips 313 is larger than the interval between the bottoms.
In this embodiment: in the biological mixed culture, the pollution of a culture container is caused after the conventional biological culture is finished, the culture container can be reused after cleaning and sterilizing, the inner side of the conventional biological culture container is generally provided with stirring blades, the cleaning and sterilizing are troublesome, the next biological culture work can be influenced due to unqualified cleaning and sterilizing, the conventional biological culture container is generally detected after cleaning, and the workload is increased;
through the biological mixing reactor 1, the cleaning-free mixing culture device can be used after the biological culture work, the organisms on the inner side of the biological reaction bag 4 can be cultured and mixed by directly putting the biological reaction bag 4 into the inner side of the mixing reaction assembly 2, the biological reaction bag 4 can be directly taken out after the culture is finished, the mixing reaction assembly 2 is not required to be cleaned, meanwhile, the mixing reaction assembly 2 has a good biological mixing function, and for microorganisms needing to be mixed in a reinforcing way, the mixing can be further reinforced through the arranged control mixing assembly 3, and the cleaning-free mixing culture device also has the cleaning-free function;
When the biological mixing reactor 1 is used for mixing and culturing microorganisms, biological materials and required auxiliary materials are placed into the disposable bag 41, then the sealing cover 42 is screwed so as to enable the disposable bag 41 to achieve a sealing state, then the disposable bag 41 is placed into a containing space surrounded by the inner wall 213 in the mixing and reacting assembly 2, and the shape of the disposable bag 41 is changed by the screw thread protrusion 214 and the central protrusion 215 due to the fact that the screw thread protrusion 214 and the central protrusion 215 are arranged on the inner wall 213, so that the disposable bag 41 also has structures similar to the screw thread protrusion 214 and the central protrusion 215, and therefore liquid on the inner side of the disposable bag 41 flows in a spiral circulation state due to the influence of the shape of the screw thread protrusion 214 and the central protrusion 215 when the inner wall 213 rotates, and a certain stirring and mixing effect is achieved;
By energizing the second driving motor 25, the second driving motor 25 is energized to drive the rotary inner housing 211 to rotate, the rotary inner housing 211 can be rotated more stably inside the reaction outer housing 21 by the first bearing 251, the rotary inner housing 211 rotates to drive the middle wall 212 and further drive the inner wall 213 to rotate, the inner wall 213 and the threaded boss 214 and the central boss 215 arranged on the inner wall 213 drive the disposable bag 41 to rotate, since the disposable bag 41 immediately rotates along with the inner wall 213, the liquid inside the disposable bag 41 can not be immediately rotated under the influence of inertia, therefore, compared with the liquid inside the disposable bag 41, the disposable bag 41 can be rotated under the influence of the threaded boss 214 and the central boss 215, the threaded boss 214 and the central boss 215 change the shape of the disposable bag 41, the structure of the similar threaded boss 214 on the disposable bag 41 can enable the inner side bag 41 to immediately rotate along with the inner wall 213, the part of the threaded boss 214 moves upwards along with the inner wall 213 or the bottom of the threaded boss 213, and the top of the liquid can be moved upwards along with the inner wall 213, the top of the threaded boss 213 is moved upwards along with the inner wall 213 or the bottom of the threaded boss 213, the top of the threaded boss 213 is moved upwards along with the top of the inner wall 213, the center bulge 215 can move the liquid with the axis downwards to a position close to the inner wall 213 for recirculation, and the same principle is that when the liquid near the inner wall 213 moves downwards in a spiral manner to reach the bottom of the inner wall 213, the liquid moves upwards after gathering along the shape of the center bulge 215 towards the axis position of the inner wall 213, and when the liquid at the axis position of the inner wall 213 moves upwards to reach the top liquid level, the liquid moves downwards after moving towards the inner wall 213 under the influence of the thread bulge 214, so as to recirculate;
In order to make the screw protrusions 214 and the central protrusions 215 provided on the inner wall 213 perform a better function on the disposable bag 41, namely, after the disposable bag 41 is placed in the accommodating space of the inner wall 213, the shape of the disposable bag 41 can be better attached to the inner wall 213, the screw protrusions 214 and the central protrusions 215, so that the shape of the disposable bag 41 can be better changed, the shape of the disposable bag 41 is more similar to the shape of the inner wall 213, the screw protrusions 214 and the central protrusions 215, and simultaneously, the disposable bag 41 can be better rotated along with the inner wall 213, so that the disposable bag 41 cannot be deformed or partially separated from the inner wall 213, the screw protrusions 214 and the central protrusions 215, by pumping out air between the middle wall 212 and the inner wall 213, air negative pressure can occur after the partial air is pumped out, on the basis that the disposable bag 41 is placed inside the inner wall 213, and the disposable bag 41 can be tightly adhered to the outer surface of the inner wall 213 by the first through hole 216 provided, but the air between the middle wall 212 and the inner wall 213 is directly drawn out at one time, while the disposable bag 41 can be tightly adhered to the outer surfaces of the inner wall 213, the screw boss 214 and the central boss 215 and can be more adhered to the shapes of the inner wall 213, the screw boss 214 and the central boss 215, the part of the disposable bag 41 which is in contact with the inner wall 213, the screw boss 214 and the central boss 215 first can be adhered first, and the part which is not adhered can be forcibly stretched and adhered under the condition of air negative pressure, which can cause the part of the disposable bag 41 to be adhered to the inner wall 213, the screw boss 214 and the central boss 215 after being stretched and can also cause the part of the disposable bag 41 to be still not be adhered to the inner wall 213 after being stretched and thinned, the threaded boss 214 and the central boss 215 are attached, and the disposable bag body 41 after stretch-thinning is fragile and has a risk of breakage;
In order to solve the problem, the space between the middle wall 212 and the inner wall 213 is divided into a plurality of independent spaces by the arranged sealing plates 22 in a sectional mode, the first driving motor 245 is electrified and the vacuum air pump 243 is electrified, the air in the annular sleeve 24 is pumped out through the spring air pipe 242 after the first driving motor 245 is electrified, the annular sleeve 24 is covered by the annular sleeve 24 and communicated with the one-way valve 23, the one-way valves 23 in the upper and lower directions are preferably arranged at equal intervals, if a plurality of one-way valves 23 are arranged in the space between the two sealing plates 22, the one-way valves 23 in the space position are in the same plane, the air in the independent space surrounded by the two adjacent sealing plates 22 is pumped out by the one-way valve 23, and the air in different independent spaces is sequentially pumped out in the upper direction according to the same method, so that the disposable bag 41 is firstly tightly attached to the central bulge 215, then attached to the inner wall 213 in the bottom of the bag 213, the disposable bag 41 is sequentially thinned, and the disposable bag 41 is sequentially attached to the inner wall 213 and the inner wall 213 is sequentially attached to the inner wall 213, and the disposable bag 41 is tightly attached to the inner wall 213 by threads of the disposable bulge 213;
By energizing the first driving motor 245, the first driving motor 245 drives the threaded rod 246 to rotate, the threaded rod 246 rotates to drive the threaded sleeve 247 to move, the threaded sleeve 247 moves to drive the annular sleeve 24 to move, the limiting rod 241 is fixed on the outer surface of the middle wall 212, the limiting rod 241 can limit the moving range of the annular sleeve 24, the check valve 23 can use the existing check valve 23, the check valve 23 can also be composed of a valve body housing 231, a fixed ventilation plate 232, a sliding rod 233, a hard plate 234, a sealing ring 235, a spring seat 236 and a compression spring 237, the outer end face outer side of the valve body housing 231 is fixedly connected to the inner side of the middle wall 212 between two adjacent sealing plates 22, the valve body housing 231 can penetrate through the middle wall 212, the inner side of one end of the valve body housing 231 is fixedly connected with the fixed ventilation plate 232, one end of the fixed ventilation plate 232 is provided with a ventilation hole, the sliding rod 233 is slidingly connected to the central position of the inner side of one end of the fixed ventilation plate 232, the hard plate 234 is fixedly connected to one end of the sliding rod 233, the sealing ring 235 is fixedly connected to the position, close to the plane of the fixed ventilation plate 232, of the hard plate 234, the sealing ring 235 can be in contact with the fixed ventilation plate 232 and block the vent hole of the fixed ventilation plate 232, the spring seat 236 is fixedly connected to the other end of the sliding rod 233, the compression spring 237 is fixedly connected between the outer side of one end of the spring seat 236 and the outer side of one end of the fixed ventilation plate 232, when the air in the independent space between the two sealing plates 22 is extracted, the air passes through the vent hole of the fixed ventilation plate 232 and pushes the sealing ring 235 to move, the hard plate 234 is driven to move after the sealing ring 235 moves, the sliding rod 233 moves, the spring seat 236 is driven to move against the elastic force of the compression spring 237, moving the grommet 235 out of contact with the fixed ventilation plate 232 allows air to pass through the valve body housing 231 in a single item, but when air passes through the valve body housing 231 in the reverse direction, the air cannot push the grommet 235 away from the fixed ventilation plate 232, so that in this direction the air cannot pass through the valve body housing 231;
In order to make the disposable bag 41 have better stirring and mixing effects, the stirring rod 43 is placed inside the disposable bag 41, the hollow part 433 is arranged at the top end of the stirring rod 43, and the metal rod 432 is arranged inside the buffering flexible layer 431 of the stirring rod 43, so that the hollow part 433 of the stirring rod 43 can float on the liquid surface inside the disposable bag 41, the buffering flexible layer 431 at the outer part of the metal rod 432 is positioned inside the liquid, and the metal rod 432 capable of being attracted and moved by magnetic force is arranged inside the stirring rod 43, so that when the electromagnet 314 is electrified, the stirring rod 43 can rapidly move towards the electrified electromagnet 314, and when a plurality of circularly arranged electromagnets 314 are powered off after being electrified in an unordered short time, the stirring rod 43 can irregularly move inside the disposable bag 41, thereby enhancing the stirring effect;
The control mixing assembly 3 is matched with the mixing reaction assembly 2, the collecting shell 31 is sleeved on the outer side of the top end of the reaction outer shell 21, the control mixing assembly 3 and the mixing reaction assembly 2 can be fastened together through the threaded connection of the internal threaded part 341 arranged on the collecting shell 31 and the external threaded part 342 arranged on the reaction outer shell 21, when the top of the reaction outer shell 21 is contacted with the bottom of the control shell 311 through the fixed sealing piece 34 arranged at the bottom of the control shell 311, good tightness is realized, meanwhile, the damage to the shell is avoided, the third driving motor 322 is electrified, the rotating wheel 323 is driven to rotate after the third driving motor 322 is electrified, the pushing plate 324 is driven to rotate, the conductive balls 316 on the inner side of the circulation track 32 are pushed by the rotating plate 324, so that the conductive balls 316 on the inner side of the circulation track 32 are upwards moved, finally fall to the top end of the damping plate 332 from the top end of the circulation track 32, and the conductive balls 316 can be prevented from bouncing after falling through the damping plate 332;
Embodiment one: by energizing the fourth driving motor 33, the fourth driving motor 33 can drive the connecting bracket 331 to drive the dispersion housing 315 to rotate, the dispersion housing 315 can drive the shock absorbing plate 332 to rotate, the dispersion housing 315 can be rotated more stably through the second bearing 333, the shaking is reduced, the second bearing 333 can be fixedly installed through the bearing bracket 334, when the shock absorbing plate 332 rotates, when one conductive ball 316 falls at the center position of the shock absorbing plate 332 which is rotating at a uniform speed, the conductive ball 316 can be rotated by the rotation of the shock absorbing plate 332 to follow the rotation, So that the conductive ball 316 rotates, since the conductive ball 316 is unlikely to be positioned at the center of the shock absorbing plate 332 without error, the conductive ball 316 gradually moves away from the center of the shock absorbing plate 332 and finally rolls off from the upper side of the shock absorbing plate 332 as time passes, the closer the conductive ball 316 is to the center of the shock absorbing plate 332, the longer the rotation time of the conductive ball 316 remains at the center of the shock absorbing plate 332, conversely, the farther the initial position of the conductive ball 316 on the shock absorbing plate 332 is from the center of the shock absorbing plate 332, the shorter the time of the conductive ball 316 remains at the center of the shock absorbing plate 332, the diameter of the circulation track 32 is slightly larger than the diameter of the conductive ball 316, Thus, when the conductive balls 316 are dropped from the top end of the circulation rail 32 to the upper side of the shock absorbing plate 332, the position is very close to the center of the shock absorbing plate 332, but the distance between the conductive balls 316 and the center of the shock absorbing plate 332 is random, when one conductive ball 316 stays on the shock absorbing plate 332 without immediately dropping, and the next conductive ball 316 is dropped from the top end of the circulation rail 32, the next conductive ball 316 is hit against the previous conductive ball 316, so that the previous conductive ball 316 is forcibly hit away from the shock absorbing plate 332, thereby increasing the randomness of the direction in which the conductive ball 316 drops from the shock absorbing plate 332, The conductive balls 316 are separated from the shock absorbing plate 332 and then roll down to the bottom of the control housing 311 along the outer surface of the dispersion housing 315, and since the bottom of the control housing 311 is provided with the second through holes 312, the conductive balls 316 fall from the second through holes 312, so that the specific second through holes 312 from which the different conductive balls 316 fall is random, the conductive balls 316 fall from the second through holes 312 and then contact with the pair of conductive semicircular strips 313, the pair of adjacent semicircular strips 313 capable of being enclosed into an approximately cylindrical shape are formed, when the conductive balls 316 electrically contact with the pair of semicircular strips 313, The conductive ball 316 acts as a conductive body to electrically connect the two semicircular strips 313, so as to realize the energizing of a specific electromagnet 314, when the conductive ball 316 is separated from the semicircular strips 313 after continuously falling under the influence of gravity, the electromagnet 314 is deenergized, that is, when the conductive ball 316 falls randomly from a certain second through hole 312 and is electrically contacted with a certain semicircular strip 313, the electromagnet 314 is deenergized after being temporarily energized, and due to the continuous random falling of a plurality of conductive balls 316, the plurality of electromagnets 314 are randomly energized, the electromagnet 314 can cause the stirring rod 43 to rapidly approach the energized electromagnet 314, When the plurality of electromagnets 314 are randomly energized, the stirring rod 43 is randomly moved inside the disposable bag 41, and the liquid inside the disposable bag 41 can be stirred by the stirring rod 43 by the random movement of the stirring rod 43;
In the second embodiment, on the basis of the first embodiment, the fifth driving motor 355 is energized, the fifth driving motor 355 drives the gear 354 to rotate, the gear 354 rotates to drive the gear ring 353 to rotate, the gear ring 353 rotates to drive the limiting ring 35 to rotate along the annular bracket 352, since only one third through hole 351 is formed on the limiting ring 35, the conductive ball 316 can fall from the inner side of the control housing 311 to contact with the semicircular strip 313 only when the third through hole 351 and the second through hole 312 overlap, in this embodiment, the rotation speed of the third driving motor 322 should be increased appropriately, so that the number of conductive balls 316 entering the inner side of the control housing 311 is increased in the same time period, but the conductive balls 316 should be prevented from accumulating at the annular bottom of the control housing 311, in the case where the restriction ring 35 rotates, even if the conductive ball 316 has fallen to the annular bottom of the control housing 311, it cannot immediately fall, but only when the third through hole 351 and the second through hole 312 overlap, the conductive ball 316 falls, and the overlapping portion of the third through hole 351 and the second through hole 312 moves in a circumferential track, so that the conductive ball 316 falls successively in the circumferential track, and according to the above principle, the energization sequence of the electromagnet 314 also energizes in the circumferential track, so that the stirring rod 43 is not irregularly mixed, but the buffer flexible layer 431 rotates around the hollow portion 433 of the stirring rod 43, and the rotation track of the stirring rod 43 is tapered or frustum-shaped, so that a sequential stirring effect is obtained;
When the conductive balls 316 fall from the semicircular bars 313, they fall to the bottom of the collecting housing 31, and the bottom of the collecting housing 31 is inclined, so that the conductive balls 316 roll down to the lowest part of the collecting housing 31 and then enter the inner side of the circulating track 32 to realize circulation;
the top ends of the semicircular strips 313 are set to rotate by taking the rotating bracket 36 as the center of rotation, the bottoms of the semicircular strips 313 can be inclined appropriately through the reset spring 361, the top end ring of the pair of semicircular strips 313 is slightly larger than the bisection section of the conductive balls 316, and the bottom end ring of the pair of semicircular strips 313 is slightly smaller than the bisection section of the conductive balls 316, so that the conductive balls 316 can be in better electrical contact with the semicircular strips 313, and good electrifying effect is maintained.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (4)

1.A wash-free bioreactor vessel, characterized in that: the biological mixing reactor (1) comprises a mixing reaction assembly (2) and a biological reaction bag (4);
The mixing reaction assembly (2) comprises a reaction outer shell (21), a rotation inner shell (211), a middle wall (212), an inner wall (213), a threaded protrusion (214), a central protrusion (215) and a first through hole (216), wherein the rotation inner shell (211) is rotationally connected to the inner side of the reaction outer shell (21), the inner wall (213) is fixedly connected to one end of the rotation inner shell (211), the middle wall (212) is fixedly connected to a gap between the rotation inner shell (211) and the inner wall (213), the threaded protrusion (214) is arranged at one end of the inner wall (213), the central protrusion (215) is arranged at the central position of the bottom end of the inner wall (213), and the first through hole (216) is formed in the inner side of one end of the inner wall (213);
The biological reaction bag (4) comprises a disposable bag body (41) and a sealing cover (42), wherein the sealing cover (42) is detachably connected to an opening at one end of the disposable bag body (41), and the disposable bag body (41) can be attached to the surface of the inner wall (213) by pumping out air between the middle wall (212) and the inner wall (213);
The biological reaction bag (4) further comprises a stirring rod (43) arranged on the inner side of the disposable bag body (41), the stirring rod (43) comprises a buffering flexible layer (431), a metal rod (432) and a hollow part (433), the hollow part (433) is arranged at the top end of the buffering flexible layer (431), and the metal rod (432) is fixedly connected on the inner side of one end of the buffering flexible layer (431);
The biological mixing reactor (1) further comprises a control mixing assembly (3), the control mixing assembly (3) comprises a collecting shell (31), a control shell (311), a second through hole (312), semicircular strips (313), electromagnets (314), a dispersing shell (315) and conductive balls (316), wherein the annular hollow collecting shell is arranged at the bottom, the semicircular strips (313) are inclined at the bottom, the top end of the collecting shell (31) is fixedly connected with the control shell (311), the second through holes (312) are formed in the bottom end of the control shell (311) at equal intervals, two semicircular strips (313) are arranged right below the position of each second through hole (312), each two adjacent semicircular strips (313) can be simultaneously in electrical contact with one conductive ball (316), a plurality of electromagnets (314) are fixed on the inner wall (213) surface of the rotating inner shell (211) at equal intervals, the dispersing shell (315) is arranged on the inner side of the control shell (311), and the dispersing shell (316) can enable any conductive ball (316) to pass through any second through hole (312);
the control mixing assembly (3) further comprises a circulating track (32), a motor support (321), a third driving motor (322), a rotating wheel (323) and a pushing plate (324), wherein the top end of the circulating track (32) is fixedly communicated with the inner side of the central position of the top end of the control shell (311), the bottom end of the circulating track (32) is fixedly communicated with the outer side of the bottom end of the collecting shell (31), the outer side of one end of the circulating track (32) is fixedly connected with the motor support (321), the inner side of one end of the motor support (321) is fixedly connected with the third driving motor (322), the tail end of a main shaft of the third driving motor (322) is fixedly connected with the rotating wheel (323), the outer side of the rotating wheel (323) is fixedly connected with a plurality of pushing plates (324), the pushing plates (324) extend to the inner side of one end of the circulating track (32), and the pushing plates (324) rotate around the rotating wheels (323) to push conductive balls (316) to move;
The control mixing assembly (3) further comprises a fourth driving motor (33), a connecting support (331), a damping plate (332), a second bearing (333) and a bearing support (334), wherein the bottom end of the fourth driving motor (33) is fixedly connected to the central position of the inner side of the bottom end of the control shell (311), the connecting support (331) is fixedly connected to the tail end of a main shaft of the fourth driving motor (33), one end outer side of the connecting support (331) is fixedly connected with one end of the dispersing shell (315), the damping plate (332) is fixedly connected to the upper side of the top end plane of the dispersing shell (315), the second bearing (333) is fixedly connected to the outer side of the bottom of the dispersing shell (315), and one end of the second bearing (333) is fixedly connected to the inner side of the bottom of the control shell (311) through the bearing support (334);
The control mixing assembly (3) further comprises a limiting ring (35), a third through hole (351), an annular support (352), a gear ring (353), a gear (354) and a fifth driving motor (355), wherein the annular support (352) is fixedly connected to the outer side of the bottom end of the control shell (311), the limiting ring (35) is rotatably connected to the inner side of the annular support (352), the third through hole (351) is formed in one end of the limiting ring (35), the gear ring (353) is fixedly embedded into the inner side of one end of the limiting ring (35), one end of the fifth driving motor (355) is fixedly connected to the outer side of one end of the collection shell (31), the gear (354) is fixedly connected to the tail end of a main shaft of the fifth driving motor (355), and one end outer side of the gear (354) is in meshed connection with one end outer side of the gear ring (353).
The control mixing assembly (3) further comprises a rotating support (36) and a reset spring (361), the top end of the semicircular strip (313) is rotatably connected with the inner side of one end of the collecting shell (31) through the rotating support (36), the reset spring (361) is fixedly connected between the outer end face of the bottom end of the rotating support (36) and the inner wall (213) face of the collecting shell (31), and two semicircular strips (313) which are formed into a shape similar to a circular tube are obliquely arranged, and the distance between the tops of the semicircular strips (313) is larger than that between the bottoms.
2. The rinse-free bioreactor vessel of claim 1, wherein: the mixing reaction assembly (2) further comprises a sealing plate (22) and a one-way valve (23), wherein the sealing plate (22) is fixedly connected between the middle wall (212) and the inner wall (213), the sealing plate (22) divides the space between the middle wall (212) and the inner wall (213) into a plurality of independent spaces, the sealing plate (22) is not overlapped with the first through hole (216), and the one-way valve (23) is fixedly communicated with the inner side of the middle wall (212).
3. The rinse-free bioreactor vessel of claim 1, wherein: the mixing reaction assembly (2) further comprises an annular sleeve (24), a spring air pipe (242), a vacuum air pump (243), a first driving motor (245), a threaded rod (246) and a threaded sleeve (247), wherein the outer end face of the middle wall (212) is slidably connected with the annular sleeve (24), one end inner side of the annular sleeve (24) is fixedly communicated with the spring air pipe (242), the bottom end of the spring air pipe (242) is fixedly connected with an air suction nozzle of the vacuum air pump (243), one end of the vacuum air pump (243) is fixedly connected with the outer side of the bottom end face of the rotary inner shell (211), the outer side of the bottom end face of the rotary inner shell (211) is fixedly connected with the first driving motor (245), the spindle end of the first driving motor (245) is fixedly connected with the threaded rod (246), one end outer side of the threaded rod (246) is in threaded connection with the threaded sleeve (247), and one end outer side of the threaded sleeve (247) is fixedly connected with one end outer side of the annular sleeve (24).
4. The rinse-free bioreactor vessel of claim 1, wherein: the mixing reaction assembly (2) further comprises a second driving motor (25) and a first bearing (251), the outer side of the bottom end of the second driving motor (25) is fixedly connected with the inner side of the bottom end of the reaction outer shell (21), the tail end of a main shaft of the second driving motor (25) is fixedly connected with the central position of the outer side of the bottom end of the rotation inner shell (211), and the rotation inner shell (211) is rotatably connected with the inner side of the reaction outer shell (21) through the first bearing (251).
CN202310509343.XA 2023-05-08 2023-05-08 Cleaning-free biological reaction container Active CN116731845B (en)

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CN108544458A (en) * 2018-05-28 2018-09-18 江苏聚泰科技有限公司 A kind of supporter of mobile phone screen

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