CN115043674B - Method for quick decomposition and drying of fresh banana straw - Google Patents

Method for quick decomposition and drying of fresh banana straw Download PDF

Info

Publication number
CN115043674B
CN115043674B CN202210574676.6A CN202210574676A CN115043674B CN 115043674 B CN115043674 B CN 115043674B CN 202210574676 A CN202210574676 A CN 202210574676A CN 115043674 B CN115043674 B CN 115043674B
Authority
CN
China
Prior art keywords
fermentation
composite
microbial inoculum
fresh banana
bacillus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210574676.6A
Other languages
Chinese (zh)
Other versions
CN115043674A (en
Inventor
吴金川
吴海洋
李清心
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Biological and Medical Engineering of Guangdong Academy of Sciences
Original Assignee
Institute of Biological and Medical Engineering of Guangdong Academy of Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Biological and Medical Engineering of Guangdong Academy of Sciences filed Critical Institute of Biological and Medical Engineering of Guangdong Academy of Sciences
Priority to CN202210574676.6A priority Critical patent/CN115043674B/en
Publication of CN115043674A publication Critical patent/CN115043674A/en
Application granted granted Critical
Publication of CN115043674B publication Critical patent/CN115043674B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Physiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Processing Of Solid Wastes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a method for quick decomposition and drying of fresh banana straws. The method comprises the steps of mixing and solidifying a composite microbial inoculum and wheat bran or other solid organic wastes until no liquid flows out, fermenting at the temperature of 30-50 ℃ to obtain the composite microbial inoculum, mixing the composite microbial inoculum with fresh banana straw, and thoroughly decomposing and drying the composite microbial inoculum, wherein the composite microbial inoculum contains bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens and lactobacillus fermentum. The method comprises the steps of utilizing a composite solid microbial inoculum containing bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens and lactobacillus fermentum to rapidly ferment, dehydrate and dry fresh banana straws within 24 hours at a temperature of 30-50 ℃, discharging after 10-15 batches to obtain organic fertilizer or animal feed with qualified decomposition degree, and generating no wastewater and odor in the whole treatment process. The popularization and application of the technology can realize the high-efficiency, low-cost, zero-pollution and high-valued treatment of the agricultural wastes with high water content such as banana straw, and the like, and can generate remarkable economic, environmental and social benefits after the industrialized application.

Description

Method for quick decomposition and drying of fresh banana straw
Technical field:
The invention belongs to the technical field of microorganisms, and particularly relates to a method for efficiently and rapidly decomposing and drying fresh banana straws to be used as fertilizer or feed.
The background technology is as follows:
According to statistics, the annual generation amount of banana straw in China is about 2400 ten thousand tons. The banana straw has water content of more than 85%, can not be directly combusted to be used as energy, and can be decomposed after being piled for several hours at room temperature due to high organic matter content, and bad smell is generated, so that serious environmental problems are caused.
The invention comprises the following steps:
The invention aims to solve the technical problems of overcoming the defects of the prior art in converting banana straws into fertilizers and feeds, developing the concepts of realizing quick decomposition and desiccation of banana straws by utilizing microbial fermentation, converting nutrients in banana straws into animal feeds or organic fertilizers, realizing the recycling of organic wastes, protecting the environment and assisting the realization of ecological cycle agriculture.
The invention aims to provide a method for quickly decomposing and drying fresh banana straws, which effectively avoids the problem of environmental pollution caused by natural decay of banana straws, and efficiently and quickly converts the banana straws into organic fertilizer or animal feed so as to improve the comprehensive utilization efficiency of organic wastes in banana industry.
The invention relates to a method for quick decomposition and drying of fresh banana straw, which comprises the steps of mixing and solidifying a composite microbial inoculum and wheat bran or other solid organic waste until no liquid flows out, fermenting at the temperature of 30-50 ℃ to obtain a composite solid microbial inoculum, mixing the composite solid microbial inoculum and the fresh banana straw, and decomposing and drying the composite solid microbial inoculum;
The composite microbial inoculum contains bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens and lactobacillus fermentum.
Preferably, in the process of mixing the composite solid microbial inoculum and the fresh banana straw to thoroughly decompose and dry the composite solid microbial inoculum, part or all of the materials can be removed within 10-15 days to be used as feed or fertilizer, or the fresh banana straw is continuously added to perform the next fermentation.
The preparation method of the composite microbial inoculum comprises the following steps:
activating strains, inoculating a seed culture medium, culturing for 24 hours at 37 ℃ and 180rpm to obtain seed liquid, inoculating the seed liquid to a fermentation culture medium according to the volume ratio of 4%, fermenting at 37 ℃ with ventilation of 300-350L/h, and fermenting and culturing for 48 hours to obtain fermentation liquid of each strain;
and mixing fermentation liquor of bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens and lactobacillus fermentum according to the volume ratio of 1:1:1:1 to prepare the composite microbial inoculum.
The seed culture medium and the fermentation culture medium of the bacillus subtilis, the bacillus licheniformis and the bacillus amyloliquefaciens comprise 10g/L of peptone, 3g/L, naCl g/L of beef extract, pH is 6.0-8.0, and the solvent is water. The seed culture medium and the fermentation culture medium of the lactobacillus fermentum are prepared from 10g/L of peptone, 5g/L of beef extract, 4g/L of yeast extract powder, 20g/L of glucose, 1ml/L of tween-80, 2g/L of dipotassium hydrogen phosphate, 5g/L of sodium acetate, 2g/L of triammonium citrate, 0.2g/L of magnesium sulfate (MgSO 4.7H2O), 0.05g/L of manganese sulfate (MnSO 4·4H2 O) and 6.0-8.0 of pH value, wherein the solvent is water.
The dosage ratio of the composite microbial inoculum to the wheat bran is that 0.67kg of wheat bran is added per liter of composite microbial inoculum.
The composite solid microbial inoculum is obtained by fermenting at 30-50 ℃ for 24 hours at 40 ℃ under stirring at 20 rpm.
Preferably, the mixing of the composite solid microbial inoculum and the fresh banana straw is that after the fresh banana straw with the mass of 0.5-3.0 times of that of the composite solid microbial inoculum is cut into pieces every 24 hours, the pieces are poured into a solid fermentation reactor for fermentation, the temperature is 30-50 ℃, and the stirring speed is 5-25rpm.
After 10-15 days, the solid state fermentation material in the solid state fermentation reactor is removed from the solid state fermentation reactor and is directly used as feed or fertilizer or is stored in a dry and ventilated place for standby, and the fresh banana straw can also be continuously added for the next round of solid state fermentation.
The method comprises the steps of utilizing a composite solid microbial inoculum containing bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens and lactobacillus fermentum to rapidly ferment, dehydrate and dry fresh banana straws within 24 hours at a temperature of 30-50 ℃, discharging after 10-15 batches to obtain organic fertilizer or animal feed with qualified decomposition degree, and generating no wastewater and odor in the whole treatment process. The popularization and application of the technology can realize the high-efficiency, low-cost, zero-pollution and high-valued treatment of the agricultural wastes with high water content such as banana straw, and the like, and can generate remarkable economic, environmental and social benefits after the industrialized application.
Description of the drawings:
FIG. 1 is a solid state microbial inoculum of individual strains of banana straw solid state fermentation treatment of banana straw;
fig. 2 is a composite solid microbial agent solid state fermentation treatment of banana straw.
Fig. 3 is a seed germination index experiment of the organic fertilizer prepared by solid state fermentation treatment of the composite solid state microbial inoculum of banana straw.
The specific embodiment is as follows:
Specific examples of the present invention will be described below, but the examples are not intended to limit the present invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
EXAMPLE 1 preparation of the seed Strain
The strain comprises bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and lactobacillus fermentum (Lactobacillus fermentum).
Bacillus subtilis may produce highly active alkaline proteases or acidic proteases or both. Bacillus licheniformis can produce antibacterial lipopeptides and can produce high-activity cellulase or hemicellulase or both. Bacillus amyloliquefaciens can produce highly active starch hydrolyzing enzymes and glucoamylases. Lactobacillus fermentum can secrete bacteriocin and has acid production and probiotic effects.
Activating strains, inoculating a seed culture medium, culturing for 24 hours at 37 ℃ and 180rpm to obtain seed liquid, inoculating the seed liquid to a fermentation culture medium according to the volume ratio of 4%, fermenting at 37 ℃ with ventilation of 300-350L/h, and fermenting and culturing for 48 hours to obtain fermentation liquid of each strain.
And mixing fermentation liquor of bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens and lactobacillus fermentum according to the volume ratio of 1:1:1:1 to prepare the composite microbial inoculum.
Adding 0.67kg of wheat bran into each liter of fermentation liquor for solidification, weighing to obtain the mass of initial solidified strains, transferring into a solid state fermentation reactor, and stirring and fermenting (20 rpm) at 40 ℃ for 24 hours to respectively obtain solid state bactericides and composite solid state bactericides of bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens and lactobacillus fermentum.
The seed culture medium and the fermentation culture medium of the bacillus subtilis, the bacillus licheniformis and the bacillus amyloliquefaciens comprise 10g/L of peptone, 3g/L, naCl g/L of beef extract, pH is 6.0-8.0, and the solvent is water. The seed culture medium and the fermentation culture medium of the lactobacillus fermentum are prepared from 10g/L of peptone, 5g/L of beef extract, 4g/L of yeast extract powder, 20g/L of glucose, 1ml/L of tween-80, 2g/L of dipotassium hydrogen phosphate, 5g/L of sodium acetate, 2g/L of triammonium citrate, 0.2g/L of magnesium sulfate (MgSO 4.7H2O), 0.05g/L of manganese sulfate (MnSO 4·4H2 O) and 6.0-8.0 of pH value, wherein the solvent is water. The preparation method comprises mixing the above materials, and sterilizing.
EXAMPLE 2 solid state fermentation of Banana straw by separate species
After the solid microbial agents of bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens and lactobacillus fermentum are prepared, adding a part of fresh banana straw (chopped) which is approximately equal to the mass of the solid microbial agents, and observing straw treatment conditions after 24 hours, wherein each sample is treated by 3 pieces. As a result, as shown in FIG. 1, the fermentation of the strain alone did not complete the treatment of banana stalks within 24 hours (FIG. 1 is Bacillus licheniformis, and the results of Bacillus subtilis, bacillus amyloliquefaciens and Lactobacillus fermentum alone were similar, and the treatment of banana stalks within 24 hours did not complete).
Example 3 composite seed solid State fermentation of Banana straw
After the composite solid microbial inoculant is prepared, adding fresh banana straw which is approximately equal to the composite solid microbial inoculant in mass, and adding fresh banana straw (shredding) which is approximately equal to the composite solid microbial inoculant in mass every 24 hours. 3 treatments per sample. The results are shown in FIG. 2. Compared with the solid microbial inoculum treatment of single strain, the composite solid microbial inoculum can treat banana straw within 24 hours (figure 2).
The mass before and after the addition of the strain was weighed, and the material reduction rate was calculated (Table 1).
Table 1 banana straw reduction rate
As can be seen from Table 1, the banana straw reduction rate is high, the speed is high, the method is suitable for being popularized and used in factories near large-area banana planting bases, the transportation cost of the straw is reduced, the environment is protected, and after 10 times of banana straw is added, the solid state fermentation product is obtained.
Example 4 moisture, pH, CFU and ingredient determination
After 10 banana stalks were added (example 3), the solid state fermentation was partially removed for direct application and the remaining part was stored for later use. The water content is measured to be 28.3%, the pH is 7.8, the strain density is 9.0X10 10 cfu/g, and the compound microbial fertilizer meets the national agricultural industry standard NY/T798-2015.
The details of the component measurement are shown in Table 2.
Table 2 analysis of banana straw fertiliser composition
Example 5 seed germination index determination
Seed germination rate tests were performed on the solid state fermentate prepared in example 3, with reference to national agricultural industry standard NY/T3442-2019, livestock manure composting technical specification appendix D. The germination rate of cucumber seeds is 97.65% (figure 3), and the decomposition degree is good.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit of the present invention should be made in the equivalent manner, and are included in the protection scope of the present invention.

Claims (3)

1.一种新鲜香蕉秸秆快速腐熟干化的方法，其特征在于，是将复合菌剂与麦麸混合固化至无液体流出，于40°C下20 rpm搅拌发酵24h，得到复合固态菌剂，将复合固态菌剂与新鲜香蕉秸秆混合对其进行腐熟干化；1. A method for rapidly ripening and drying fresh banana straws, characterized in that a composite bacterial agent is mixed with wheat bran and solidified until no liquid flows out, and the mixture is stirred and fermented at 20 rpm at 40°C for 24 hours to obtain a composite solid bacterial agent, and the composite solid bacterial agent is mixed with fresh banana straws to ripen and dry the mixture; 所述的复合菌剂的制备方法为：The preparation method of the composite bacterial agent is as follows: 先活化菌种，接入种子培养基在37°C、180 rpm下培养24h得种子液，然后以体积比4%接种种子液到发酵培养基，发酵温度为37°C，通气量为300~350 L/h，发酵培养48 h，得各菌种发酵液；First, the strains were activated, inoculated into the seed culture medium and cultured at 37°C and 180 rpm for 24 h to obtain the seed solution, and then the seed solution was inoculated into the fermentation medium at a volume ratio of 4%, the fermentation temperature was 37°C, the ventilation volume was 300-350 L/h, and the fermentation culture was carried out for 48 h to obtain the fermentation solution of each strain; 将枯草芽孢杆菌、地衣芽孢杆菌、解淀粉芽孢杆菌、发酵乳杆菌的发酵液按体积比1:1:1:1混合制得复合菌剂；The fermentation broths of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens and Lactobacillus fermentum are mixed in a volume ratio of 1:1:1:1 to prepare a composite bacterial agent; 枯草芽孢杆菌、地衣芽孢杆菌、解淀粉芽孢杆菌的种子培养基和发酵培养基的配方为：蛋白胨10 g/L、牛肉浸出物3 g/L、NaCl 5 g/L，pH 6.0~8.0，溶剂为水；发酵乳杆菌的种子培养基和发酵培养基的配方为：蛋白胨10 g/L、牛肉浸出物5 g/L、酵母膏粉4 g/L、葡萄糖20 g/L、吐温-80 1 ml/L、磷酸氢二钾2 g/L、乙酸钠5 g/L、柠檬酸三铵2 g/L、硫酸镁0.2g/L、硫酸锰0.05 g/L, pH 6.0~8.0，溶剂为水；The formula of seed culture medium and fermentation medium of Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens is: peptone 10 g/L, beef extract 3 g/L, NaCl 5 g/L, pH 6.0-8.0, and the solvent is water; the formula of seed culture medium and fermentation medium of Lactobacillus fermentum is: peptone 10 g/L, beef extract 5 g/L, yeast extract powder 4 g/L, glucose 20 g/L, Tween-80 1 ml/L, potassium dihydrogen phosphate 2 g/L, sodium acetate 5 g/L, ammonium citrate trihydrate 2 g/L, magnesium sulfate 0.2 g/L, manganese sulfate 0.05 g/L, pH 6.0-8.0, and the solvent is water; 所述的复合菌剂与麦麸的用量比是每升复合菌剂添加0.67kg麦麸。The dosage ratio of the composite bacterial agent to the wheat bran is 0.67 kg of wheat bran per liter of the composite bacterial agent. 2.根据权利要求1所述的方法，其特征在于，在复合固态菌剂与新鲜香蕉秸秆混合对其进行腐熟干化过程中，10~15 天内可移除部分或全部物料作饲料或肥料，或继续添加新鲜香蕉秸秆进行下一轮发酵。2. The method according to claim 1 is characterized in that, during the process of mixing the composite solid bacterial agent with fresh banana straw for composting and drying, part or all of the material can be removed as feed or fertilizer within 10 to 15 days, or fresh banana straw can be continued to be added for the next round of fermentation. 3.根据权利要求1所述的方法，其特征在于，所述的将复合固态菌剂与新鲜香蕉秸秆混合是每24 h将0.5-3.0倍复合固态菌剂质量的新鲜香蕉秸秆切碎片后倒入固态发酵反应器中发酵，温度为30-50°C，搅拌速率为5-25rpm。3. The method according to claim 1 is characterized in that the mixing of the composite solid-state inoculant with fresh banana straw comprises cutting fresh banana straw of 0.5-3.0 times the mass of the composite solid-state inoculant into pieces and pouring them into a solid-state fermentation reactor for fermentation at a temperature of 30-50°C and a stirring rate of 5-25rpm every 24 hours.
CN202210574676.6A 2022-05-24 2022-05-24 Method for quick decomposition and drying of fresh banana straw Active CN115043674B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210574676.6A CN115043674B (en) 2022-05-24 2022-05-24 Method for quick decomposition and drying of fresh banana straw

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210574676.6A CN115043674B (en) 2022-05-24 2022-05-24 Method for quick decomposition and drying of fresh banana straw

Publications (2)

Publication Number Publication Date
CN115043674A CN115043674A (en) 2022-09-13
CN115043674B true CN115043674B (en) 2024-11-29

Family

ID=83160059

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210574676.6A Active CN115043674B (en) 2022-05-24 2022-05-24 Method for quick decomposition and drying of fresh banana straw

Country Status (1)

Country Link
CN (1) CN115043674B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115819122A (en) * 2022-12-08 2023-03-21 广东省科学院生物与医学工程研究所 A method for rapidly decomposing and drying sisal residue

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113735652A (en) * 2021-08-27 2021-12-03 河北昱鼎生物科技有限公司 Efficient organic material decomposing agent and preparation method and application thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103214282B (en) * 2013-04-28 2014-08-20 山东省农业科学院农业资源与环境研究所 Microbial organic fertilizer and preparation method thereof
CN103642726B (en) * 2013-11-28 2015-09-30 天津海友佳音生物科技股份有限公司 A kind of maize straw organism of fermentation microbial inoculum and preparation method thereof and fermentation process
CN107325990B (en) * 2017-08-14 2020-11-06 山东巴迪生物科技股份有限公司 Composite microorganism decomposing agent and application thereof in tobacco cultivation
CN110093300A (en) * 2019-05-22 2019-08-06 北京师范大学 A kind of method of bacillus composite bacteria agent of degrading maize straws and preparation method thereof, degrading straw
CN111517841A (en) * 2020-06-18 2020-08-11 河南农之宝农业科技有限公司 Organic material decomposing agent and decomposing method
AU2020103489A4 (en) * 2020-11-17 2021-01-28 Changchun University Of Technology A straw-decomposing bacterial agent and its application
CN114015619A (en) * 2021-12-09 2022-02-08 北京市畜牧业环境监测站 Straw fermentation composite bacterium preparation and preparation method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113735652A (en) * 2021-08-27 2021-12-03 河北昱鼎生物科技有限公司 Efficient organic material decomposing agent and preparation method and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"农作物秸秆生物发酵机理与饲养价值评定";毛建红等;《饲料工业》;第38卷(第21期);第14-18页 *

Also Published As

Publication number Publication date
CN115043674A (en) 2022-09-13

Similar Documents

Publication Publication Date Title
CN109402015B (en) A strain of Bacillus thermoamylophagus and its application
CN107177533B (en) A kind of thermophilic bacteria compound bacterial agent and its preparation method and application
CN105154373A (en) High-temperature cow dung decomposition agent and preparation method thereof
CN114480215B (en) Compound microbial agent and application thereof in biogas residue high-temperature aerobic composting
CN111533586B (en) A kind of chicken manure biological organic fertilizer and preparation method thereof
CN108486010A (en) A kind of During High-Temperature Composting bacterium composite bacteria agent and its application
CN113234616A (en) Bacillus licheniformis, saline-alkali tolerant microbial agent and preparation method of biomass saline-alkali soil conditioner
CN104312947A (en) Multi-strain efficient straw-decomposing inoculant and preparation method and application thereof
CN113234446A (en) Biomass saline-alkali soil conditioner
CN115125168B (en) A compound bacterial enzyme agent for treating kitchen waste and its preparation method and application
CN115044503A (en) Composite microbial inoculum for producing high-temperature protease, high-temperature amylase and high-temperature lipase as well as preparation method and application thereof
CN104509683A (en) Method for preparing yellow corn silage feed by compounding and fermenting shells of bamboo shoots
CN104293719B (en) Fast decomposing agent for fermentation bed aging padding, organic fertilizer and production method of organic fertilizer
CN106947490A (en) Soil conditioner and preparation method thereof
CN106146218B (en) A kind of preparation method of soil improvement liquid and soil improvement liquid
CN108203352A (en) A kind of production method of kitchen garbage organic fertilizer
CN115043674B (en) Method for quick decomposition and drying of fresh banana straw
WO2019237656A1 (en) Special fertilizer for medicinal plant growth and preparation method therefor
CN102334589A (en) Method for preparing fermented feed from waste bacillus fermentation liquor
CN115819122A (en) A method for rapidly decomposing and drying sisal residue
CN108192845B (en) Enzyme-bacteria compound for harmless treatment of animal carcasses and preparation method thereof
CN101993832A (en) Composite bacterial agent for pig manure compost treatment and application method
CN108410772A (en) A kind of preparation method of straw biological decomposing agent
CN106954736A (en) The method that ruminant compound microecological active biological fodder is produced using disused mushroom dregs
CN102173879A (en) Method for producing biological potassium fertilizer by utilizing cellulose fermented waste mycelium and biogas residue

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant