CN114085776B

CN114085776B - Wall-attached cell culture system

Info

Publication number: CN114085776B
Application number: CN202111406469.1A
Authority: CN
Inventors: 张锴; 宋志兵; 蔡罗强; 吴定; 冯振雄; 丁正科; 张舒乐
Original assignee: Hunan Kaiqi Shidai Biotechnology Co ltd
Current assignee: Hunan Kaiqi Shidai Biotechnology Co ltd
Priority date: 2021-11-24
Filing date: 2021-11-24
Publication date: 2024-09-24
Anticipated expiration: 2041-11-24
Also published as: CN114085776A

Abstract

The invention relates to an adherent cell culture system, comprising: a support table, an incubator device embedded in the support table, a culture dish device supported on the support table, a touch display device, a control unit, a container carrying table and an interface unit; the culture dish device includes: the device comprises a feeding unit, a collecting unit, a cell culture dish, a main pipeline, a first pipe pressure valve and a peristaltic pump; the cell culture dish is positioned in the incubator device; the collecting unit is connected with the container bearing table; the first pipe pressure valve and the peristaltic pump are arranged on the main pipe at intervals; the feeding unit is connected with the main pipeline, and the connection position of the feeding unit and the main pipeline is positioned at the upstream of the first pipe pressure valve; the collecting unit is connected with the main pipeline, and the connecting position of the collecting unit and the main pipeline is positioned between the first pipe pressure valve and the peristaltic pump; the end part of the main pipeline, which is positioned at the downstream of the peristaltic pump, passes through the incubator device and is integrally arranged with the end cover of the cell culture dish, and the end face of the end part does not exceed the inner side face of the end cover.

Description

Wall-attached cell culture system

Technical Field

The invention relates to an adherent cell culture system.

Background

Cell culture is a method established in the early twentieth century to study animal cell behavior by taking cells from tissues in vivo, simulating the growth environment in vivo, growing and propagating the cells under aseptic, appropriate temperature, ph and nutritional conditions, and maintaining the structure and function thereof. Cell preparation refers to the process that scientific researchers culture high-purity target cell end products by using a relatively mature cell culture scheme, and can be directly applied to scientific research and clinical fields after functional and quality detection.

The operation such as feeding and draining in the cell preparation process is very important, and the current cell preparation process usually adopts a manual operation mode to realize the liquid feeding and draining operation, and the operation process usually needs to be realized by opening a cell culture dish, so that the environment in the culture dish is easily polluted, and the cell culture efficiency is seriously affected.

Disclosure of Invention

The invention aims to provide an adherent cell culture system.

To achieve the above object, the present invention provides an adherent cell culture system comprising: a support table, an incubator device embedded in the support table, a culture dish device supported on the support table, a touch display device, a control unit, a container carrying table and an interface unit;

The culture dish device includes: the device comprises a feeding unit, a collecting unit, a cell culture dish, a main pipeline, a first pipe pressure valve and a peristaltic pump;

the cell culture dish is positioned in the incubator device;

The collecting unit is connected with the container bearing table;

the first pipe pressure valve and the peristaltic pump are arranged on the main pipeline at intervals;

The feeding unit is connected with the main pipeline, and the connection position of the feeding unit and the main pipeline is positioned at the upstream of the first pipe pressure valve;

the collecting unit is connected with the main pipeline, and the connecting position of the collecting unit and the main pipeline is positioned between the first pipe pressure valve and the peristaltic pump;

The end part of the main pipeline, which is positioned at the downstream of the peristaltic pump, passes through the incubator device and is integrally arranged with the end cover of the cell culture dish, and the end surface of the end part does not exceed the inner side surface of the end cover.

According to one aspect of the invention, the feed unit comprises: a plurality of first containers for storing nutrient solution, a plurality of second containers for storing enzyme, a first branch pipe connected with the first containers, a second branch pipe connected with the second containers, and branch pipe pressure valves respectively arranged on the first branch pipe and the second branch pipe;

On the main pipeline, the first branch pipe and the second branch pipe are arranged in a staggered way at intervals;

the collecting unit includes: the collecting pipes are connected with the collecting containers and used for collecting pipes connected with the main pipelines, and collecting pipe pressure valves are respectively arranged on the collecting pipes;

One end of the collecting branch pipe is connected with the collecting container, and the other end of the collecting branch pipe is connected with the collecting pipe;

The collecting container comprises a collecting container cover and a collecting container body detachably connected with the collecting container cover;

The end of the collecting branch pipe is integrally arranged with the collecting container cover.

According to one aspect of the invention, the cell culture dish further comprises: the bottle body is detachably connected with the end cover;

the bottle includes: a bottle body part and a bottleneck part arranged at one side of the bottle body part;

The bottom part of one end of the bottle body part connected with the bottle neck part is provided with an inclined plane, and the rest part is a plane;

The edge of the inclined surface is provided with a support leg;

The bottom ends of the supporting feet are flush with the plane of the bottom of the bottle body part;

The end cover or the bottle body is provided with a medical ventilation filter membrane for ventilation and liquid resistance.

According to one aspect of the invention, the incubator apparatus comprises: the cell culture dish comprises a box body with a heating function, a box cover, a temperature and humidity adjusting device arranged on one side of the box body and positioned on the outer side of the box body, a culture dish supporting table arranged in the box body and used for bearing the cell culture dish, a microscopic device arranged at the bottom of the culture dish supporting table, and a lighting device positioned above the culture dish supporting table;

the side, connected with the temperature and humidity regulating device, of the box body is regularly provided with meshes penetrating through the side wall of the box body;

The mesh is positioned above the culture dish supporting table;

the temperature and humidity adjusting device is used for carrying out gas exchange through the meshes and the inside of the box body and is used for assisting in adjusting the temperature and humidity in the box body.

According to one aspect of the invention, the upper end of the box body is provided with an annular sealing heat insulation strip;

the cross section of the annular sealing heat insulation strip is semicircular or semi-elliptic;

at least one annular groove coaxial with the annular sealing heat insulation strip is formed in one side, far away from the box body, of the annular sealing heat insulation strip;

the side edges of the annular sealing heat insulation strips, which correspond to the positions where the box body and the box cover are in rotary connection, are provided with notches;

the main pipeline is connected with the end cover through the notch.

According to one aspect of the invention, the dish support table comprises: the cell culture dish comprises a connecting seat supported at the bottom of the box body, a lifting seat connected with the connecting seat, a driving device and a detection device for detecting the internal state of the cell culture dish;

The driving device is supported on the connecting seat;

one end of the lifting seat is a rotating connecting end which is rotationally connected with the connecting seat, and the other end of the lifting seat is a lifting connecting end which is connected with the driving device;

under the driving action of the driving device, the lifting connecting end can do lifting movement relative to the connecting seat.

According to one aspect of the invention, the connector comprises: the connecting frame body and the bottom support are arranged on the lower side of the connecting frame body;

the lifting seat is positioned on the inner side of the connecting frame body and above the bottom support;

The lifting seat is of a frame structure, and a vessel mounting position for mounting the cell culture vessel is arranged on the lifting seat;

the vessel mounting site includes: a first hollow portion and a second hollow portion communicating with each other;

the second hollow part is provided with an opening at the end part of the rotating connection end of the lifting seat;

In the width direction of the lifting seat, the lifting seat is provided with a penetrating light transmission channel at two opposite sides of the vessel installation position;

The light transmission channel is arranged at the position of the first hollow part, which is close to the lifting connecting end of the lifting seat.

According to one aspect of the invention, the driving device includes: a driver for outputting a linear displacement, a yoke connected to the driver;

The yoke comprises: a driver connecting arm and a lifting seat connecting arm;

the lifting seat connecting arm is arranged vertically to the driver connecting arm;

A linear channel is arranged on the bottom support;

one end of the lifting seat connecting arm far away from the driver connecting arm passes through the linear channel to be hinged with the lifting connecting end of the lifting seat;

the lifting seat connecting arm is positioned at the lower side of the bottom support, and one side of the lifting seat connecting arm, which is far away from the driver connecting arm, is hinged with the movable end of the driver;

the hinge position of the driver connecting arm and the driver is provided with a rubber damping structure for mute rotation of the hinge position;

the driver connecting arm is embedded with a position sensor for detecting the moving position of the driver connecting arm and a limiting structure for limiting the maximum displacement of the driver connecting arm.

According to one aspect of the invention, the microscopy apparatus comprises: a focusing unit, an optical lens mounted on the focusing unit, and a microscopic imaging unit for receiving an image in the optical lens;

The focusing unit is arranged on the bottom support of the connecting seat;

the microscopic imaging unit is arranged at the bottom of the box body and is positioned at the outer side of the box body;

The gear drive of the focusing unit is arranged at the bottom of the box body, and the gear drive is positioned at the outer side of the box body.

According to one aspect of the invention, the interface unit comprises: the device comprises a power supply interface for connecting a power supply, an oxygen interface for connecting an external oxygen source, a carbon dioxide interface for connecting an external carbon dioxide source, a nitrogen interface for connecting an external nitrogen source, a safety structure and a switch structure;

the control unit is respectively connected with the incubator device, the culture dish device, the touch display device and the interface unit.

According to one aspect of the present invention, the adherent cell culture system of the present invention enables automated operation of the cell culture process without human involvement. Meanwhile, the system can visually display the running states of all parts through the touch display device, so that the comprehensive grasp of a user on the running process and the cell culture process of the system is effectively ensured, and the high efficiency and convenience of cell culture are ensured.

According to the scheme of the invention, the system of the invention sets the incubator device and the container bearing table at the front side of the self-supporting table, thereby effectively facilitating the viewing and operation of a user on the cell culture process and effectively improving the user experience.

According to the scheme of the invention, the cell culture dish realizes the airtight construction of the cell culture dish unit by combining the structures of the feeding unit, the collecting unit, the cell culture dish, the pipeline and the like, so that the whole cell culture process can be effectively realized only by ensuring the cleanliness of the whole culture system, no additional manual participation is needed, and the risk of pollution of the culture dish caused by manual participation is effectively avoided. In addition, the structure of the invention also effectively realizes the automation in the cell culture process, and further effectively improves the culture efficiency.

According to the scheme of the invention, the cover body and the pipeline of the cell culture dish are directly and integrally arranged, so that the problem that the tightness between the pipeline and the cover body is difficult to solve during system assembly is effectively solved, and the risk that the joint position of the pipeline and the cover body is easily corroded by pollutants is also effectively avoided. In addition, the structure of the integrated arrangement of the pipeline and the cover body further facilitates the connection with the culture dish bottle body, effectively eliminates the contact to the interior of the culture dish in the connection process, greatly ensures the cleanliness of the whole system, and is beneficial to ensuring the normal whole cycle of the cell culture process.

According to one scheme of the invention, the whole sealing performance is excellent, and the method is beneficial to ensuring the stable and clean of the whole culture dish.

Drawings

FIG. 1 is a perspective view schematically showing an adherent cell culture system according to an embodiment of the invention;

FIG. 2 is a front view schematically illustrating an adherent cell culture system according to one embodiment of the invention;

FIG. 3 is a rear view schematically illustrating an adherent cell culture system according to one embodiment of the invention;

FIG. 4 is a block diagram schematically showing a culture dish device according to an embodiment of the present invention;

FIG. 5 is a block diagram schematically illustrating a cell culture dish according to an embodiment of the invention;

FIG. 6 is a perspective view schematically showing an incubator assembly according to one embodiment of the present invention;

FIG. 7 is a view schematically showing an internal structure of an incubator assembly according to an embodiment of the present invention;

FIG. 8 is a top view schematically showing an incubator assembly according to one embodiment of the present invention;

FIG. 9 is a bottom view schematically showing an incubator assembly according to one embodiment of the present invention;

FIG. 10 is a block diagram schematically illustrating an annular sealing insulation blanket in accordance with one embodiment of the present invention;

FIG. 11 is a schematic representation of an arrangement of a culture dish support table and a microscopy apparatus according to an embodiment of the invention;

FIG. 12 is a side view schematically illustrating a culture dish support according to one embodiment of the invention;

FIG. 13 is a block diagram schematically showing a dish support table according to one embodiment of the present invention;

FIG. 14 is a bottom view schematically showing a culture dish support table according to one embodiment of the invention;

Fig. 15 is a structural view schematically showing a lifting seat according to an embodiment of the present invention.

Detailed Description

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments will be briefly described below. It is apparent that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained from these drawings without inventive effort for a person of ordinary skill in the art.

In describing embodiments of the present invention, the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer" and the like are used in terms of orientation or positional relationship based on that shown in the drawings, which are merely for convenience of description and to simplify the description, rather than to indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and thus the above terms should not be construed as limiting the present invention.

The present invention will be described in detail below with reference to the drawings and the specific embodiments, which are not described in detail herein, but the embodiments of the present invention are not limited to the following embodiments.

Referring to fig. 1,2 and 3, according to one embodiment of the present invention, an adherent cell culture system of the present invention includes: a support table 1, an incubator device 2 embedded in the support table 1, a culture dish device 3 supported on the support table 1, a touch display device 4, a control unit, an interface unit 6, and a container support table 5. In this embodiment, the control unit is connected to the incubator device 2, the culture dish device 3, the touch display device 4 and the interface unit 6, respectively, wherein the control unit can control the incubator device 2 and the culture dish device 3 through information input by the touch display device 4, and can display information collected in the incubator device 2 and the culture dish device 3 through the touch display device 4.

Through the arrangement, the adherent cell culture system can realize the automatic operation of the cell culture process, and does not need to be manually participated. Meanwhile, the system can visually display the running states of all parts through the touch display device, so that the comprehensive grasp of a user on the running process and the cell culture process of the system is effectively ensured, and the high efficiency and convenience of cell culture are ensured.

As shown in fig. 1,2 and 3, according to an embodiment of the present invention, the support table 1 has a hollow structure, the incubator 2 and the container support table 5 are disposed at a position near the front side of the support table 1, and the support bar and the touch display device 4 are disposed at a position near the rear side of the support table 1. In the present embodiment, the support bar is used to support the feeding unit 31 in the culture dish device 3 in order to achieve stable downward transport of the liquid. In the present embodiment, the support bar and the touch display device 4 are arranged side by side on the support stand 1. In the present embodiment, the control unit is located inside the support table 1. The interface unit 6 is located at the lower part of the rear side of the support table 1.

Through the arrangement, the system of the invention sets the incubator device 2 and the container bearing table 5 from the front side of the supporting table 1, thereby effectively facilitating the viewing and operation of a user on the cell culture process and effectively improving the user experience.

Referring to fig. 1, 2, 3, and 4, according to an embodiment of the present invention, the culture dish device 3 includes: a feeding unit 31, a collecting unit 32, a cell culture dish 33, a main pipeline 34, a first tube pressure valve 35 and a peristaltic pump 36. In the present embodiment, the cell culture dish 33 is located in the incubator apparatus 2; the collection unit 32 is connected to the container carrier 5. In the present embodiment, the first tube pressure valve 35 and the peristaltic pump 36 are disposed on the main tube 34 at a distance. The feeding unit 31 is connected with the main pipeline 34, and the connection position of the feeding unit 31 and the main pipeline 34 is positioned upstream of the first pipe pressure valve 35; the collecting unit 32 is connected to the main line 34, the position at which the collecting unit 32 is connected to the main line 34 is located downstream of the first tube pressure valve 35, and the position at which the collecting unit 32 is connected to the main line 34 is located between the first tube pressure valve 35 and the peristaltic pump 36. In the present embodiment, the end of the main tube 34 downstream of the peristaltic pump 36 is integrally provided with the end cap 331 of the cell culture dish 33.

According to one embodiment of the present invention, the end cap 331 is provided with a through hole, the end of the main pipe 34 connected to the end cap 331 is integrally disposed with the edge of the through hole on the inner side (i.e. the side connected to the mouth of the bottle body 332) or the outer side of the end cap 331, and the end face of the end of the main pipe 34 does not exceed the inner side surface of the end cap 331.

Through the arrangement, the cell culture dish realizes the airtight construction of the cell culture dish unit by combining the structures of the feeding unit 31, the collecting unit 32, the cell culture dish 33, the pipelines and the like, so that the whole cell culture process can be effectively realized as long as the cleanliness of the whole culture system is ensured, no additional manual participation is needed, and the risk of pollution of the culture dish caused by manual participation is effectively avoided. In addition, the structure of the invention also effectively realizes the automation in the cell culture process, and further effectively improves the culture efficiency.

Through the arrangement, the cover body and the pipeline of the cell culture dish are directly and integrally arranged, so that the problem that the tightness between the pipeline and the cover body is difficult to solve during system assembly is effectively solved, and the risk that the joint position of the pipeline and the cover body is easily corroded by pollutants is also effectively avoided. In addition, the structure of the integrated arrangement of the pipeline and the cover body further facilitates the connection with the culture dish bottle body, effectively eliminates the contact to the interior of the culture dish in the connection process, greatly ensures the cleanliness of the whole system, and is beneficial to ensuring the normal whole cycle of the cell culture process.

Through the arrangement, the culture dish has excellent overall sealing performance and is beneficial to ensuring the stable and clean interior of the whole culture dish.

As shown in connection with fig. 1,2, 3, 4, according to one embodiment of the present invention, the feeding unit 31 includes: a plurality of first containers 311 for storing nutrient solution, a plurality of second containers 312 for storing enzymes, a first branch 313 connected to the first containers 311, a second branch 314 connected to the second containers 312, and branch line pressure valves 315 provided on the first branch 313 and the second branch 314, respectively. In the present embodiment, on the main pipe 34, the first branch pipe 313 and the second branch pipe 314 have a staggered arrangement at intervals. In the present embodiment, the number of the first container 311 and the second container 312 can be adaptively adjusted and matched according to the type of the cells to be cultured, so as to meet the culture requirement. In the present embodiment, the type of the enzyme stored in the second container may be set as required, and for example, the e-enzyme for collection is selectively stored in the second container.

Through above-mentioned setting, through set up the pipe pressure valve on every branch pipe can realize the accurate control to every container confession liquid to make the accurate configuration of the nutrient solution in the cell culture process, guaranteed the normal clear of whole culture process.

Through above-mentioned setting, arrange the branch pipe through with different kinds of containers with staggered arrangement's mode on being responsible for the pipeline to can realize mixing in the pipeline when making the liquid in the different kinds of containers add through the main pipeline simultaneously, and then can effectively guarantee that the distribution of different kinds of liquid when getting into the cell culture dish is more even.

As shown in connection with fig. 1,2, 3, 4, according to one embodiment of the present invention, the collecting unit 32 includes: a plurality of collecting vessels 321, collecting branch pipes 322 connected to the collecting vessels 321, collecting pipes 323 connected to the main pipe 34, and collecting pipe pressure valves 324 provided in the collecting branch pipes 322, respectively. In the present embodiment, the collecting pipe 322 is connected to the collecting container 321 at one end and to the collecting pipe 323 at the other end. In the present embodiment, the collecting pipes 322 are arranged in a spaced-apart manner on the collecting pipe 323. In this embodiment, the number of the collection containers 321 may be set correspondingly according to the needs, so as to ensure flexibility of the present invention in practical application.

According to one embodiment of the present invention, the collection container 321 includes a collection container cover and a collection container body 3211 removably connected to the collection container cover. In the present embodiment, the end of the collecting branch pipe 322 is integrally provided with the collecting container cover. In this embodiment, the ends of the collection manifold 322 may extend into the collection container cover or may be flush with the inside surface of the collection container cover.

In this embodiment, two collection containers are provided, one for collecting the culture medium liquid after culturing in the culture dish and the other for collecting the supernatant in the culture dish.

Referring to fig. 1, 2, 3, 4, and 5, according to one embodiment of the present invention, the cell culture dish 33 further includes: a bottle 332 removably attached to the end cap 331. In the present embodiment, the bottle 332 includes: a bottle portion 3321 and a bottleneck portion 3322 provided at one side of the bottle portion 3321. In this embodiment, a bottom portion of an end of the bottle body portion 3321 connected to the bottleneck portion 3322 is provided as an inclined surface 3321a, and the remaining portion is a flat surface 3321b.

By the above arrangement, the position where the bottleneck portion 3322 is connected with the bottle portion 3321 is advantageous for the smoothness of the liquid inlet by the inclined bottle bottom portion being inclined, effectively leading the liquid to be guided to the rear position of the bottle 332, and ensuring the normal growth of cells at the rear position in the culture dish.

In the present embodiment, the edge of the inclined surface 3321a provided on the bottle 332 is provided with a leg 3321c; the bottom ends of the legs 3321c are flush with the planar surface 3321b of the bottom of the bottle portion 3321.

Through the arrangement, the flatness of the bottom of the bottle 332 is effectively guaranteed, the stable placement of the bottom of the bottle 332 is facilitated, and the stability of the culture environment is guaranteed.

According to one embodiment of the invention, the bottle 332 portion of the cell culture dish 33 is transparent. In the present embodiment, a convex lens is provided on the inclined surface 3321a of the bottle 332. In the present embodiment, the convex lens is integrally provided with the inclined surface 3321 a. Through set up the convex lens on the inclined plane, effectually realized carrying out statistics to the quantity of cell on the inclined plane and observing, the cell growth state of the non-horizontal bottom surface position of judgement that can be accurate has guaranteed the accurate grasp of whole culture dish inside culture environment.

According to one embodiment of the invention, a medical ventilation filter membrane for ventilation and liquid resistance is arranged on the end cover 31 or the bottle body 32 of the cell culture dish 3. In this embodiment, the medical breathable filter membrane can only allow gas to pass through, so as to ensure the exchange of gas in the culture dish and gas outside the culture dish. The medical ventilation filter membrane can prevent the passage of water, bacteria and other substances, so as to ensure the maintenance of culture solution in the culture dish and play a role of cleaning air.

As shown in fig. 2, a light sensation calibration device a is provided on a sidewall of the bottle 32 according to an embodiment of the present invention. Through set up light sense demarcation device A on the lateral wall of culture dish, as the demarcation device of whether the cell pollution in the culture dish is detected to the in-process of cultivateing, can cooperate the sensor to use, improve the timeliness to inside cultivation environment detection.

Referring to fig. 1,2, 6, 7, 8, and 9, according to an embodiment of the present invention, an incubator apparatus 2 includes: a case 21 having a heating function, a case cover 22, a temperature and humidity adjusting device 23 provided at one side of the case 21 and located at the outside of the case 21, a culture dish supporting table 24 provided in the case 21 for carrying a cell culture dish 33, a microscopic device 25 provided at the bottom of the culture dish supporting table 24, and an illumination device 26 located above the culture dish supporting table 24; in the present embodiment, the case 21 has a symmetrical rectangular structure as a whole. The case 21 is provided with a regular array of mesh openings 21a penetrating the side wall thereof on the side where the temperature and humidity control device 23 is connected. In the present embodiment, the mesh holes 21a are symmetrically arranged in an array on both sides of a center line perpendicular to the longitudinal direction of the case. In this embodiment, the mesh 21a is located above the dish support table 24. In the present embodiment, the temperature and humidity control device 23 performs gas exchange with the inside of the case 21 through the mesh 21a, and is used for assisting in controlling the temperature and humidity in the case 21.

In this embodiment, the case 21 has a heating function, which can heat the internal environment to ensure that the internal environment of the case has a proper cell culture temperature. And when the temperature in the box body is higher, the temperature in the box body can be regulated by stopping the heating of the box body 21 and/or by the operation of the temperature and humidity regulating device 23, so that the temperature of the environment in the box body is always kept within a reasonable range.

In this embodiment, when humidity control is required for the environment in the case, the humidity control device 23 may be operated alone to perform humidification or dehumidification on the environment in the case, so as to maintain the humidity of the environment in the case constant.

In this embodiment, the number of openings and the size of the openings of the mesh openings 21a on the case 21 are matched with the volume of the case, and the circulation of air in the case 21 by the temperature and humidity adjusting device 23 can be realized by the mesh openings 21a, without generating obvious air flow in the case, especially, the openings are formed on the upper half part of the side wall of the case 21 (i.e. above the culture dish supporting table 24) in combination with the mesh openings 21a, so that the flow of air in the case is eliminated more effectively, and the stable exchange of air is beneficial.

Through the arrangement, the cell incubator disclosed by the invention is simple in structure, and the temperature and humidity in the incubator can be controlled in an auxiliary manner directly through the temperature and humidity adjusting device 23 arranged on one side of the incubator through the meshes formed in the wall of the incubator. In addition, the temperature and humidity regulating device 23 is directly arranged on the wall of the box body 21, so that the ventilation path is effectively shortened, a ventilation pipeline is eliminated, and the accurate control of the temperature and humidity in the box body 21 is realized.

Referring to fig. 1,2,6, 7, 8, and 9, according to an embodiment of the present invention, one side of the case cover 22 is detachably rotatably connected to an upper end of one side of the case 21 to which the temperature and humidity adjustment device 23 is connected. In the present embodiment, the case cover 22 is connected to the case 21 by a detachable pin, and when a structure such as a culture dish is mounted in the case 21, the case cover 22 is detachable from the case 21, and after the culture dish is mounted, the case cover 22 is connected to the case 21 to seal the case 21. In the present embodiment, the lighting device 26 is mounted on the inner side of the case cover 22. Wherein the lighting device 26 is detachably connected to the cover 22.

As shown in fig. 6, 7, 8 and 10, according to an embodiment of the present invention, the upper end of the case 21 is provided with an annular sealing heat insulating strip 211. In the present embodiment, the cross-sectional shape of the annular seal insulating strip 211 is a semicircle or a semi-ellipse. By providing the upper side of the annular sealing insulating strip 211 with an arc shape, line contact with the case cover can be achieved, effectively making close contact with the case cover at various positions.

Through the arrangement, the annular sealing strip is arranged between the box body 21 and the box cover 22, so that a gap between the upper end face of the box body and the box cover can be effectively eliminated, and the stable maintenance of the constant temperature and humidity environment in the box body is further ensured.

In the present embodiment, at least one annular groove 211a coaxial with the annular sealing heat insulating strip 211 is provided on a side of the annular sealing heat insulating strip 211 remote from the case 21. In the present embodiment, the cross section of the annular groove 211a is rectangular. And then the side surface of the annular groove 211a is a vertical surface, and then a sharp angle is formed at the connection position between the upper side of the annular sealing heat insulation strip and the curved surface, so that the multiple sealing effect can be realized at the position where the annular sealing heat insulation strip contacts the box cover 22. In addition, the intensity of the sharp corner edge that the annular groove formed on the sealing strip is less, and then can produce little bending to the recess inboard in the position that contacts with the case lid, and then can produce more inseparabler contact with the case lid, can produce the relative slip of less distance between can and the case lid in addition when the edge produces little bending, can produce the clearance effect to the position that contacts with the case lid, guarantee the leakproofness of contact position. In addition, a plurality of annular grooves 211a are arranged at intervals which can be juxtaposed in the width direction of the annular sealing heat insulating strip 211, so that a plurality of edges with different heights can be formed on the surface of the sealing strip, and the effect of multiple sealing is achieved.

In the present embodiment, the annular sealing heat insulating strip 211 is provided with a notch 211b on the side corresponding to the position where the case 21 and the cover 22 are rotatably connected. In the present embodiment, the main pipe 34 is connected to the end cap 331 through the notch 211b, and the width of the notch 211b and the shape of the notch cross section match the shape of the cross section of the main pipe 34 passing therethrough.

Through the above arrangement, by arranging the notch 211b on the annular sealing heat insulating strip 211, the mounting and passing of the culture dish pipeline can be realized through the notch. This seals the periphery of the dish tubing by means of the annular sealing insulating strip 211. Especially under the condition that the case cover is buckled with the case body, the sealing strip can enable the end face of the notch 211b to be in closer contact with the outer side face of the pipeline due to the action of extrusion force, so that the sealing strip is beneficial to guaranteeing the air tightness around the pipeline. In addition, the notch 211b on the sealing strip can also realize the positioning function on the pipeline, and an additional clamping structure is not needed, so that the installation of the pipeline in the box body 21 is effectively simplified.

Through the arrangement, the mode of arranging the notch 211b on the annular sealing heat insulation strip 211 can conveniently and simply replace the mode of opening on the box body or the box cover, and further the defect that the air tightness of the structure is poor due to the opening on the box body or the box cover can be effectively eliminated. In addition, the mode of opening on the sealing ring is simple and convenient, and an additional sealing structure is not required to be added, so that the structure is effectively simplified under the condition of ensuring good air tightness.

As shown in fig. 1, 2, 6, 7, and 8, according to an embodiment of the present invention, the portion of the main pipe 34 extending into the tank may be directly fixed to the side of the tank 21 where the mesh 21a is provided. In the present embodiment, the piping of the main piping 34 may be extended appropriately so that it can be wound in a coil shape in a ring shape on the side of the case 21 where the mesh 21a is provided, and the main piping 34 is fixed with the center line of the formed ring structure perpendicular to the side wall of the case 21.

Through the arrangement, when the air flow output by the temperature and humidity adjusting device can be exchanged with the inside of the box body through the part of the main pipeline in the box body, the liquid in the main pipeline is subjected to advanced temperature control treatment, so that the temperature of the liquid entering the cell culture dish can be more close to the temperature of the liquid in the cell culture dish, stable growth of cells is guaranteed, and the influence of overlarge temperature difference between the external liquid temperature and the liquid in the cell culture dish is avoided.

In the present embodiment, the portion of the main pipe 34 extending into the tank may further increase the pipe diameter portion of the main pipe 34, and the pipe diameter at the end portion connected to the end cap 331 of the cell culture dish may be reduced (the length of the pipe diameter reduced portion may be set according to actual needs), and may be appropriately extended so that it may be wound in a coil shape in a ring shape at the side of the tank 21 where the mesh 21a is provided, and the center line of the formed ring structure is perpendicular to the side wall of the tank 21 to fix the main pipe 34.

Through above-mentioned setting, can further make the liquid that gets into in the culture dish have longer dwell time in the incubator to effectively solve the big problem of liquid difference in temperature, and then be beneficial to guaranteeing the normal growth of cells in the culture dish.

Referring to fig. 6,7 and 8, according to an embodiment of the present invention, the case 21 is a hollow structure surrounded by a plurality of layers of plates. In the present embodiment, the case 21 includes: the heat conduction layer, the heating layer, the heat preservation and the insulating layer are arranged from inside to outside in sequence. In this embodiment, the heat conductive layer is made of stainless steel metal plate. The heating layer is used for heating the heat conduction layer, and the heat conduction layer made of metal is convenient for transferring heat generated by the heating layer to the inside of the box 21, so that the inside of the box is subjected to constant temperature control. In this embodiment, the heating layer is coated on the outer side of the heat conducting layer, the heat insulating layer is coated on the outer side of the heating layer, and the insulating layer is coated on the outer side of the heating layer; the heating layer effectively inhibits the heat loss generated by the heating device, and is beneficial to accurately controlling the constant temperature effect of the box body. In addition, the use safety of the case 21 is also effectively ensured by the provided insulating layer.

In the present embodiment, the positions of the heat conductive layers where the mesh holes 21a are provided are covered with a sterilizing gas passing film; the sterilization and air passing film can be arranged to isolate the inside of the box body from the external environment, so that the purification effect on the gas is realized when the gas passes through the sterilization and air passing film, and the sterilization and air passing film is beneficial to inhibiting the gas flow generated by gas exchange.

The box body is of a multi-layer composite structure, is simple in structure, can be conveniently embedded into other systems, and ensures the use and installation flexibility of the box body. In addition, through setting up the box as having the zone of heating, it can be convenient realize the direct heating in the box, has eliminated the heat exchange route in the middle, the effectual contact with the external world that reduces is favorable to the accurate control of box internal temperature. Meanwhile, as the temperature and humidity regulating device 23 is directly connected with the box body, the influence of heat generated in the working process of the temperature and humidity regulating device 23 on the environment in the box body can be effectively eliminated by arranging the heat insulation layer and the insulating layer on the box body.

In the present embodiment, the cover 22 is a hollow plate-like body, and a side thereof adjacent to the case 21 is made of a heat insulating material. Through the arrangement, the cooperation with the box 21 is effectively realized, and the stability of the environment in the box is guaranteed.

In this embodiment, be provided with carbon dioxide sensor, oxygen sensor, humidity transducer, temperature sensor in box 21 for the internal environment of real-time detection box 21, and then the real-time accurate adjustment to the internal environment of box is realized to the zone of heating of accessible temperature and humidity control device 23 and box, guarantees the equilibrium of temperature and humidity in the box, avoids appearing the gradient difference in the box.

Referring to fig. 7, 8, 9, 11, 12, 13, and 14, according to one embodiment of the present invention, the dish support table 24 includes: a connection base 241 supported at the bottom of the case 21, a lifting base 242 connected to the connection base 241, a driving device 243, and a detecting device 244 for detecting the internal state of the culture dish mounted on the lifting base 242. In the present embodiment, the driving device 243 is supported by the connection base 241. One end of the lifting seat 242 is a rotation connection end which is rotatably connected with the connection seat 241, and the other end is a lifting connection end which is connected with the driving device 243. In the present embodiment, the lifting connection end can perform lifting movement relative to the connection seat 241 under the driving action of the driving device 243.

Through the above arrangement, the lifting seat 242 is used for carrying a culture dish (e.g. an adherent cell culture dish), and further, the lifting connection end of the lifting seat can be lifted vertically by the rotation connection end under the action of the driving device, so that the lifting seat 242 can perform tilting motion relative to the connection seat 241, thereby facilitating the liquid in the culture dish to flow out smoothly when the culture dish needs to be replaced, and avoiding the residue in the culture dish.

Through the arrangement, the lifting operation of the lifting seat can be automatically realized, manual participation is not needed, and the updating efficiency of liquid in the culture dish can be effectively improved.

As shown in fig. 7, 8, 9, 11, 12, 13, and 14, according to an embodiment of the present invention, the connection base 241 includes: a connection frame 2411 and a bottom support 2412 provided on the lower side of the connection frame 2411. In the present embodiment, the connection frame 2411 is a continuous rectangular frame, and its size can be adaptively adjusted according to the size of the inside of the case. In the present embodiment, the bottom support 2412 is a plate-like body, and is fixedly attached to the lower side of the connection frame 2411. In the present embodiment, the bottom support 2412 and the connection frame 2411 may be integrally provided, or may be fixed by adhesion, screw connection, or the like.

In the present embodiment, a plurality of legs are provided at intervals on the lower side of the connection frame 2411. The height of the support legs can be adjusted according to the requirement. In the present embodiment, the connection frame 2411 is attached to the bottom of the case 21 by legs. In this embodiment, the support legs can be fixed to the bottom of the case 21 by means of connectors to achieve a fixed mounting of the culture dish support table 24.

As shown in fig. 7, 8, 9, 11, 12, 13, 14, and 15, according to one embodiment of the present invention, the lifting base 242 is located inside the connection frame 2411 and above the bottom support 2412, and in this embodiment, two opposite sides of the rotation connection end of the lifting base 242 are respectively provided with a rotation shaft 242a for rotationally connecting with the connection frame 2411. The rotation shaft 242a is connected to the lifting base 242 and the connection frame 2411 by bearings mounted on the lifting base 242 and the connection frame 2411, so as to flexibly rotate the lifting base 242 relative to the connection frame 2411. In the present embodiment, in order to connect the lifting base 242 to the inside of the connection frame 2411, the outer shape of the lifting base 242 is adapted to the shape of the inside of the connection frame 2411, and if the inside of the connection frame 2411 is rectangular, the outer shape of the lifting base 242 should be rectangular.

Referring to fig. 11, 12, 13, and 15, according to an embodiment of the present invention, the elevating base 242 has a frame structure having a vessel mounting position 242b for mounting the cell culture dish 33. In the present embodiment, the elevating base 242 has a hollow frame structure, and the vessel mounting position 242b for the culture dish is formed by the hollow structure of the elevating base 242. Specifically, the vessel mounting site 242b includes: the first hollow portion 242b1 and the second hollow portion 242b2 communicating with each other. In the present embodiment, the second hollow portion 242b2 and the first hollow portion 242b1 are disposed in this order along the direction (i.e., the length direction) from the rotational connection end of the elevating seat 242 to the elevating connection end, wherein the second hollow portion 242b2 has an opening at the end of the rotational connection end of the elevating seat 242. In this embodiment, the widths of the first hollow portion 242b1 and the second hollow portion 242b2 are different, so that a step structure is formed on the vessel mounting position 242b, which is further beneficial to the mounting and positioning of the culture dish in the first hollow portion 242b1, and ensures the mounting stability of the culture dish, while the second hollow portion 242b2 corresponds to the bottleneck and bottle cap structures on the culture dish, and realizes the stable mounting of the whole culture dish.

In the present embodiment, the second hollow portion 242b2 is formed with an opening at the end of the rotation connection end, so that interference with the structures such as the bottleneck portion 3322 and the end cap 331 on the cell culture dish 33 can be further avoided, installation of the bottleneck portion 3322 and the end cap 331 with different sizes can be adapted, and sufficient space is provided for avoiding interference between the whole culture dish supporting table 24 and the culture dish during lifting of the lifting base 242.

Through the arrangement, the vessel mounting position 242b is of a hollow structure, so that the interference with the culture dish can be effectively reduced, and the stable mounting of the culture dish is facilitated. In addition, by setting the vessel mounting position 242b to be hollow, the quality of the lifting seat can be greatly reduced, the structure of the lifting seat is simplified, and the long-time stable operation of the invention is ensured. In addition, by providing the vessel mounting portion 242b as hollow, it is also advantageous to mount other optical devices on the dish support 24 of the present invention for real-time observation of the state in the dish, effectively improving the flexibility of use of the present invention.

In the present embodiment, the lower side of the end portion of the rotation connection end of the elevating seat 242 is provided with an arc surface for connecting the end surface and the lower side surface of the rotation connection end, thereby forming a transition portion at the end portion position. The arc surface is arranged at the lower side of the end part of the rotation connecting end of the lifting seat 242, which is beneficial to ensuring the smooth rotation of the lifting seat 242 and further ensuring the use stability of the invention.

As shown in fig. 11, 12, 13, 14, and 15, according to an embodiment of the present invention, the elevating seat 242 is provided with light transmission channels 2421 penetrating through opposite sides of the vessel installation site 242b in the width direction of the elevating seat 242; in the present embodiment, the light-transmitting channel 2421 is disposed at a position where the first hollow portion 242b1 is close to the lift connection end of the lift base 242. In this embodiment, the light transmission channel 2421 is coaxially disposed on the frame of the lifting seat 242, and then, when the fixation of the culture dish is completed, the light transmission channel 2421 can be coaxially disposed on two opposite sides of the culture dish, and then, the light can be transmitted through the light transmission channel 2421 on one side, and then can pass through the culture dish to reach the light transmission channel 2421 on the other side, so that the internal state (such as the internal environment, the cell growth state, the pollution condition, the turbidity degree of the nutrient solution, etc.) of the culture dish can be collected, so that the subsequent detection and analysis can be facilitated. .

In the present embodiment, in the thickness direction of the lifting base 242, the light-transmitting channel 2421 is disposed near the lower side of the lifting base 242, that is, the light-transmitting channel 2421 is spaced from the lower side by a smaller distance than the connecting channel is spaced from the upper side.

Through the above arrangement, the light transmission channel 2421 is arranged at the position close to the lifting connection end of the lifting seat 242, so that the internal environment of the rear part of the culture dish can be detected in real time, and the internal environment of the culture dish can be accurately detected.

As shown in connection with fig. 13 and 15, according to one embodiment of the present invention, the bottom of the elevating seat 242 is provided with a support structure 2422. In this embodiment, the support structure 2422 includes: a first support part 2422a and a second support part 2422b. The first support part 2422a is provided along the inner side edge of the bottom of the first hollow part 242b 1; the second support part 2422b is connected to part of the inner side edges of the opposite sides of the bottom of the second hollow part 242b 2. In the present embodiment, the first support part 2422a is a long bar-shaped structure, which is continuously or intermittently provided at the inner side edge of the bottom of the first hollow part 242b 1. In the present embodiment, the second support part 2422b is a plate-shaped body, and opposite ends thereof are fixedly connected to inner side edges of portions of opposite sides of the bottom of the second hollow part 242b2, respectively. Effective support of the dish edge and bottleneck portion is achieved by providing support structures 2422 at the bottom edge of the dish mounting locations 242 b.

In the present embodiment, the second support part 2422b is provided with a space from the end opening of the rotation connection end. The interval between the second support part 2422b and the end opening of the rotation connection end can play a role of avoidance, avoid interference with other structures, and is beneficial to ensuring the normal and stable operation of the present invention.

As shown in fig. 11, 13, and 14, according to an embodiment of the present invention, the driving device 243 includes: a driver 2431 for outputting linear displacement, and a yoke 2432 connected to the driver 2431. In this embodiment, the yoke 2432 includes: a driver connecting arm 2432a and a lifting seat connecting arm 2432b; the lifting base connecting arm 2432b is disposed perpendicular to the driver connecting arm 2432 a. In the present embodiment, the driver connecting arm 2432a and the lifting seat connecting arm 2432b may be integrally provided, or may be connected by screwing, bonding, caulking, or the like. In the present embodiment, two elevating seat connecting arms 2432b are provided, which are connected in parallel to the driver connecting arm 2432 a.

As shown in connection with fig. 11, 12, 14, according to one embodiment of the present invention, a linear channel 2412a is provided on the bottom support 2412. In this embodiment, the end of the lift base connecting arm 2432b remote from the driver connecting arm 2432a is hinged to the lift connecting end of the lift base 242 through the linear channel 2412a. In this embodiment, a hinge seat for connecting with the driver connecting arm 2432a is provided at the lower side of the lifting connecting end, and the hinge seat is formed by two parallel plate-shaped bodies arranged at intervals, and the connection can be completed by sequentially passing through the hinge seat and the corresponding mounting position on the driver connecting arm 2432a through the hinge shaft.

In this embodiment, the lifting seat connecting arm 2432b is positioned on the underside of the bottom support 2412 and one side remote from the driver connecting arm 2432a is hinged to the movable end of the driver 2431. In the present embodiment, a first notch is disposed on the bottom support 2412 below the rotating connection end of the lifting seat 242, and a second notch is disposed at a position corresponding to the first notch on the connection frame 2411 of the connection seat 241, where the second notch is in communication with the first notch, and further the driving device 243 may be disposed at the positions of the first notch and the second notch. In the present embodiment, the driver 2431 is a linear driving device, and the telescopic end thereof can move in a linear direction, so that the free end of the driver 2431 is hinged with the driver connecting arm 2432a to realize the pushing action on the lifting seat 242.

According to one embodiment of the invention, the driver 2431 is realized by an electric cylinder, the displacement of the telescopic end of the driver 2431 can be accurately controlled by an electric signal, and the accurate control of the inclination angle of the lifting seat can be automatically realized by the control of the electric signal, so that the micro flow of nutrient solution in the culture dish and the flexible adjustment of the horizontal position of the nutrient solution can be realized. The driver connecting arm 2432a is disposed at the lower side of the bottom support 2412, and the lifting seat 242 is disposed at the upper side of the bottom support 2412, so that the bottom support 2412 can support and limit the driver connecting arm 2432a during the lifting operation of the lifting seat 242 by connecting the driver 2431 with the driver connecting arm 2432a, thereby facilitating the stable operation of the driver 2431. In addition, through the setting, can realize the accurate control to elevating seat 242 inclination in a flexible way through the flexible length of control driver 2431 free end, and need not other complicated structures can realize, realized the running accuracy height, stability is good and advantage such as simple structure.

In the present embodiment, a rubber damping structure is provided at the hinge position of the driver connecting arm 2432a and the driver 2431 for silent rotation of the hinge position. In this embodiment, damping structures made of rubber are arranged on two sides of the hinge position or on the hinge shaft to realize silencing of the hinge position, and smooth and stable operation and accurate and beneficial operation position assurance are effectively guaranteed.

In the present embodiment, the driver connecting arm 2432a is embedded with a position sensor for detecting the movement position thereof. The accurate control of the fork arm 2432 position can be realized through the real-time detection of the position of the driver connecting arm 2432a by the position sensor, so that the accurate control of the inclination angle of the lifting seat can be realized.

In the present embodiment, a stopper structure for restricting the maximum displacement of the actuator connecting arm 2432a is further provided on the actuator connecting arm 2432 a. Excessive movement of the driver connecting arm 2432a can be effectively avoided by changing the limit structure, so that the maximum lifting height of the lifting seat 242 can be effectively restrained, and stable operation of the lifting seat can be ensured, and interference with other structures can be eliminated.

Referring to fig. 11 and 12, according to an embodiment of the present invention, a detecting device 244 for detecting the internal state of the culture dish mounted on the lifting base 242 is provided on the connection frame 2411. In this embodiment, the correlation detection device is used as the detection device. Specifically, the detecting device 244 includes: a laser light source 2441, an optical fiber 2442, and an optical receiver 2443. In the present embodiment, mounting through holes penetrating the frame are provided on opposite sides of the connection frame 2411, respectively, and the laser light source 2441 and the optical receiver 2443 can be mounted in the mounting through holes so as to face each other. The optical fibers 2442 are respectively embedded in the light-transmitting channels 2421 of the lifting seat 242; when the lifting seat 242 is horizontally positioned in the connection frame 2411, the laser light source 2441, the optical fiber 2442 and the optical receiver 2443 are aligned, the light emitted by the laser light source 2441 can be transmitted to the optical receiver 2443 through the optical fiber 2442, so that the environment in the culture dish can be detected, and when the lifting seat 242 is inclined, the lifting connection end leaves the connection frame 2411, so that the detection device 244 can output a lifting signal of the lifting seat 242, and the environment in the culture dish cannot be detected. In this embodiment, the optical receiver 2443 may process and analyze the received signal by itself, or may transmit the signal to other devices for processing and analysis, and may be set according to practical applications.

Through the arrangement, the automatic detection of the culture dish can be realized by adopting the detection device to detect the culture dish. In addition, the automatic control of the lifting seat can be realized through the on-off of the detection device, and the control accuracy of the lifting seat is improved.

In addition, the optical fiber 2442 can be abutted against the culture dish according to the requirement, so that a certain pre-tightening effect can be provided for the culture dish to be beneficial to the reliable fixing of the culture dish, and the arrangement mode is more beneficial to the situation that the lifting seat is in an inclined state, and the problem that the culture dish is laterally turned over due to the fact that liquid in the culture dish gathers at the bottleneck end can be effectively avoided through the abutted pre-tightening effect on the rear part of the culture dish, so that the invention is more reliable in operation. In addition, the optical fiber 2442 and the culture dish can be integrally arranged to further play a role in fixedly connecting the culture dish while eliminating the influence of the contact position on the detection result.

As shown in connection with fig. 11, 12, 13, 14, according to one embodiment of the present invention, a first opening is provided in the bottom support 2412 through the bottom support 2412, in this embodiment, below the first hollow portion 242b 1. In this embodiment, the size of the first opening can be adjusted as needed to accommodate the installation of different structures.

Referring to fig. 9, 11, and 14, according to an embodiment of the present invention, a microscope device 25 includes: a focusing unit 251, an optical lens mounted on the focusing unit 251, and a microscopic imaging unit 252 for receiving an image in the optical lens. In the present embodiment, the focusing unit 251 is mounted on the bottom support 2412 of the connection base 241, and the microscopic imaging unit 252 is located below the bottom support 2412. In the present embodiment, the focusing unit 251 is located at the lower side of the bottom support 2412, which includes: a lens holder 2511, a gear set 2512 for adjusting the vertical position of the lens holder 2511, and a gear drive 2513 for driving the gear set 2512 to rotate. In this embodiment, the lens mount 2511 is in the position of the first opening of the bottom support 2412, which focuses the internal state of the transparent dish through the mounted optical lens for transmission to the microscopic imaging unit 252.

As shown in connection with fig. 9, 11, and 14, according to one embodiment of the present invention, the microscopic imaging unit 252 is installed at the bottom of the case 21, and the microscopic imaging unit 252 is located at the outside of the case 21. In the present embodiment, a second opening is provided at the bottom of the case 21 for allowing transmission of an image in the optical lens to the microscopic imaging unit 252. In this embodiment, the location where the microimaging unit 252 is connected to the second opening needs to be sealed.

In the present embodiment, the gear drive 2513 of the focusing unit 251 is mounted at the bottom of the housing 21, and the gear drive 2513 is located outside the housing 21.

In the present embodiment, the focusing unit 251 has compact structure and high focusing precision by the focusing mode of the gear set 512, while the microscopic imaging unit 52 adopts a compact microscopic imaging structure, which has small volume and less influence on the space required for installing the whole cell incubator, thus ensuring the installation flexibility of the cell incubator of the present invention,

According to one embodiment of the invention, the microscopy apparatus 25 further comprises: a first planar displacement structure. In the present embodiment, the focusing unit 251 is connected below the bottom support 2412 by a first plane displacement structure; the first plane displacement structure is used for driving the focusing unit 251 to linearly reciprocate in a horizontal plane. In this embodiment, the first planar displacement structure includes: the first X-axis drive and the first Y-axis drive are supported on the first X-axis drive, the focusing unit is mounted on the first Y-axis drive, and the reciprocating movement of the focusing unit in the horizontal direction is realized through the combined action of the first X-axis drive and the first Y-axis drive.

In this embodiment, to ensure the movement range of the focusing unit, the size of the first opening may be set to a larger opening area according to the movement range, so as to ensure that the focusing unit can perform focus detection on each position in the culture dish.

In this embodiment, to ensure that the focusing unit is stably and effectively input to the microscopic imaging unit 252, the microscopic device 25 further includes: prismatic structures for transmitting images. In this embodiment, the prism structure includes: the first prism is supported on the first plane displacement structure, the second prism is supported on the second opening position, and the middle prism group is used for transmitting the image in the first prism to the second prism. By the prism structure provided as described above, it is achieved that the image formed by the optical lens in the focusing unit 251 can be stably input to the microscopic imaging unit 252.

In the present embodiment, the lighting device 26 is provided with a second planar displacement structure for driving the lighting device 26 to linearly reciprocate in a horizontal plane. In this embodiment, the second planar displacement structure includes: the second X-axis drive and the second Y-axis drive are supported on the second X-axis drive, the focusing unit is mounted on the second Y-axis drive, and the reciprocating movement of the focusing unit in the horizontal direction is realized through the combined action of the second X-axis drive and the second Y-axis drive.

In this embodiment, the first planar displacement structure and the second planar displacement structure are linked.

Through above-mentioned setting, through setting up lighting device and focusing unit to the linkage, the effectual clear formation of image to each part of culture dish that has realized is favorable to comprehensive and clear acquisition cell growth state in the culture dish, has guaranteed the comprehensive accurate grasp of the information of whole cell growth cycle to can further provide relevant information to processing system, realize the accurate adjustment to nutrient solution etc. in the culture dish.

As shown in fig. 3, according to an embodiment of the present invention, the interface unit 6 includes: a power interface 61 for connecting to a power source, an oxygen interface 62 for connecting to an external oxygen source, a carbon dioxide interface 63 for connecting to an external carbon dioxide source, a nitrogen interface 64 for connecting to an external nitrogen source, and a safety structure 65 and a switch structure 66. In this embodiment, the input of the external air source and the power source is realized through the interface unit, so as to ensure the normal operation of the whole system. In this embodiment, the air source interfaces (i.e. the oxygen interface 62, the carbon dioxide interface 63, and the nitrogen interface 64) are connected with the connectors on the incubator device 2, and the control unit is used to control the opening and closing of the connectors, so as to control the input air in the cell culture process.

As shown in fig. 1, according to an embodiment of the present invention, a container carrying table 5 is provided on a supporting table 1 side by side with an incubator assembly 2. In the present embodiment, the container carrying platform 5 is an open platform for carrying the collection container 321 of the collection unit 32. When there are a plurality of collection containers 321, they are disposed side by side on the container stage 5.

According to one embodiment of the invention, one end of the container carrying table 5 is rotatably connected with the supporting table 1, and the other end is connected with a lifting structure for driving the container carrying table to lift. In this embodiment, the lifting structure is connected with the control unit, and the lifting height of the lifting structure can be controlled by the control unit, so that the control of the inclination angle of the container carrying table 5 is realized, the collection container 321 can receive the input liquid more easily, the smooth inflow and inflow speed of the liquid are ensured, and the liquid input efficiency is effectively improved.

According to an embodiment of the present invention, the temperature and humidity adjustment device 23 includes: an air conditioning unit and an air purifying unit. In this embodiment, the temperature and humidity of the internal environment of the case 21 are adjusted in an auxiliary manner by the air conditioning unit, and the internal environment of the case is further sterilized and purified by the air purifying unit, so that the cleanness of the entire case environment is further ensured.

The foregoing is merely exemplary of embodiments of the invention and, as regards devices and arrangements not explicitly described in this disclosure, it should be understood that this can be done by general purpose devices and methods known in the art.

The above description is only one embodiment of the present invention, and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An adherent cell culture system, comprising: a support table (1), an incubator device (2) embedded in the support table (1), a culture dish device (3), a touch display device (4), a control unit, a container support table (5) and an interface unit (6) supported on the support table (1);

The culture dish device (3) comprises: a feeding unit (31), a collecting unit (32), a cell culture dish (33), a main pipeline (34), a first pipe pressure valve (35) and a peristaltic pump (36);

The cell culture dish (33) is located in the incubator device (2);

The collecting unit (32) is connected with the container carrying table (5);

The first pipe pressure valve (35) and the peristaltic pump (36) are arranged on the main pipeline (34) at intervals;

the feeding unit (31) is connected with the main pipeline (34), and the connection position of the feeding unit (31) and the main pipeline (34) is positioned at the upstream of the first pipe pressure valve (35);

The collecting unit (32) is connected with the main pipeline (34), and the connection position of the collecting unit (32) and the main pipeline (34) is positioned between the first pipe pressure valve (35) and the peristaltic pump (36);

An end part of the main pipeline (34) positioned at the downstream of the peristaltic pump (36) penetrates through the incubator device (2) and is integrally arranged with an end cover (331) of the cell culture dish (33), and the end face of the end part does not exceed the inner side face of the end cover (331);

The incubator device (2) comprises: the cell culture dish comprises a box body (21) with a heating function, a box cover (22), a temperature and humidity regulating device (23) arranged on one side of the box body (21) and positioned on the outer side of the box body (21), a culture dish supporting table (24) arranged in the box body (21) and used for bearing the cell culture dish (33), a microscopic device (25) arranged at the bottom of the culture dish supporting table (24), and an illumination device (26) positioned above the culture dish supporting table (24);

The culture dish support (24) comprises: a connection base (241) supported at the bottom of the case (21), a lifting base (242) connected to the connection base (241), a driving device (243), and a detecting device (244) for detecting the internal state of the cell culture dish (33) in the width direction;

The drive device (243) is supported on the connecting seat (241);

One end of the lifting seat (242) is a rotating connecting end which is rotationally connected with the connecting seat (241), and the other end of the lifting seat is a lifting connecting end which is connected with the driving device (243);

Under the driving action of the driving device (243), the lifting connecting end can do lifting movement relative to the connecting seat (241);

The connection base (241) includes: a connection frame (2411) and a bottom support (2412) provided on the lower side of the connection frame (2411);

the lifting seat (242) is positioned on the inner side of the connecting frame body (2411) and above the bottom support (2412);

in the width direction of the lifting seat (242), the lifting seat (242) is provided with a penetrating light transmission channel (2421) on two opposite sides of the vessel installation position (242 b);

The drive device (243) includes: a driver (2431) for outputting a linear displacement, a yoke (2432) connected to the driver (2431);

The yoke (2432) comprises: a driver connecting arm (2432 a) and a lifting seat connecting arm (2432 b);

The lifting seat connecting arm (2432 b) is arranged perpendicular to the driver connecting arm (2432 a);

-said bottom support (2412) is provided with a linear channel (2412 a);

one end of the lifting seat connecting arm (2432 b) far away from the driver connecting arm (2432 a) penetrates through the linear channel (2412 a) to be hinged with the lifting connecting end of the lifting seat (242);

the lifting seat connecting arm (2432 b) is positioned at the lower side of the bottom support (2412), and one side far away from the driver connecting arm (2432 a) is hinged with the movable end of the driver (2431);

a rubber damping structure is arranged at the hinge position of the driver connecting arm (2432 a) and the driver (2431) and used for mute rotation of the hinge position;

the driver connecting arm (2432 a) is embedded with a position sensor for detecting the moving position of the driver connecting arm and a limiting structure for limiting the maximum displacement of the driver connecting arm (2432 a);

the detection device (244) comprises: a laser light source (2441), an optical fiber (2442), an optical receiver (2443);

the laser light source (2441) and the optical receiver (2443) are oppositely arranged in the mounting through holes on two opposite sides of the connecting frame body (2411);

The optical fibers (2442) are respectively embedded in the light-transmitting channels (2421) on two opposite sides of the lifting seat (242);

when the lifting seat (242) is horizontally positioned in the connecting frame body (2411), the laser light source (2441), the optical fiber (2442) and the optical receiver (2443) are aligned.

2. An adherent cell culture system according to claim 1, wherein the feed unit (31) comprises: a plurality of first containers (311) for storing nutrient solutions, a plurality of second containers (312) for storing enzymes, a first branch pipe (313) connected to the first containers (311), a second branch pipe (314) connected to the second containers (312), and branch pipe pressure valves (315) provided on the first branch pipe (313) and the second branch pipe (314), respectively;

-on the main pipe (34), the first branch pipe (313) and the second branch pipe (314) have a spaced staggered arrangement;

The collection unit (32) includes: a plurality of collection containers (321), a collection branch pipe (322) connected to the collection containers (321), a collection pipe (323) connected to the main pipe (34), and collection pipe pressure valves (324) provided in the collection branch pipes (322), respectively;

one end of the collecting branch pipe (322) is connected with the collecting container (321), and the other end is connected with the collecting pipe (323);

the collecting container (321) comprises a collecting container cover and a collecting container body (3211) detachably connected with the collecting container cover;

The end of the collecting branch pipe (322) is integrally arranged with the collecting container cover.

3. The adherent cell culture system according to claim 2, wherein the cell culture dish (33) further comprises: a bottle (332) detachably connected to the end cap (331);

The bottle (332) includes: a bottle body part (3321) and a bottleneck part (3322) arranged at one side of the bottle body part (3321);

The bottom part of one end of the bottle body part (3321) connected with the bottle neck part (3322) is provided with an inclined surface (3321 a), and the rest part is a plane (3321 b);

The edge of the inclined surface (3321 a) is provided with a support leg (3321 c);

The bottom ends of the support legs (3321 c) are flush with a plane (3321 b) at the bottom of the bottle body part (3321);

The end cover (331) or the bottle body (332) is provided with a medical ventilation filter membrane for ventilation and liquid resistance.

4. An adherent cell culture system according to claim 3, wherein the side of the case (21) to which the temperature and humidity regulating device (23) is connected is regularly arranged with mesh openings (21 a) penetrating the side wall thereof;

The mesh (21 a) is positioned above the culture dish supporting table (24);

The temperature and humidity adjusting device (23) is used for carrying out gas exchange with the inside of the box body (21) through the mesh (21 a) and is used for assisting in adjusting the temperature and humidity in the box body (21).

5. The adherent cell culture system according to claim 4, wherein an annular sealing heat insulating strip (211) is provided at an upper end of the case (21);

the cross section of the annular sealing heat insulation strip (211) is semicircular or semi-elliptic;

at least one annular groove (211 a) coaxial with the annular sealing heat insulation strip (211) is arranged on one side, far away from the box body (21), of the annular sealing heat insulation strip (211);

The side edges of the annular sealing heat insulation strips (211) corresponding to the positions where the box body (21) and the box cover (22) are in rotary connection are provided with notches (211 b);

the main pipeline (34) is connected with the end cover (331) through the notch (211 b).

6. The adherent cell culture system of claim 5 wherein,

The lifting seat (242) is of a frame structure, and a vessel mounting position (242 b) for mounting the cell culture vessel (33) is arranged on the lifting seat;

the vessel mounting site (242 b) includes: a first hollow portion (242 b 1) and a second hollow portion (242 b 2) that communicate with each other;

The second hollow part (242 b 2) is provided with an opening at the end part of the rotation connecting end of the lifting seat (242);

The light transmission channel (2421) is arranged at a position of the first hollow part (242 b 1) close to the lifting connection end of the lifting seat (242).

7. The adherent cell culture system according to claim 6, wherein the microscopy device (25) comprises: a focusing unit (251), an optical lens mounted on the focusing unit (251), a microscopic imaging unit (252) for receiving an image in the optical lens;

the focusing unit (251) is mounted on a bottom support (2412) of the connecting seat (241);

The microscopic imaging unit (252) is arranged at the bottom of the box body (21), and the microscopic imaging unit (252) is positioned at the outer side of the box body (21);

a gear drive (2513) of the focusing unit (251) is mounted at the bottom of the case (21), and the gear drive (2513) is located outside the case (21).

8. An adherent cell culture system according to any one of claims 1 to 7, wherein the interface unit (6) comprises: a power supply interface (61) for connecting a power supply, an oxygen interface (62) for connecting an external oxygen source, a carbon dioxide interface (63) for connecting an external carbon dioxide source, a nitrogen interface (64) for connecting an external nitrogen source, and a safety structure (65) and a switch structure (66);

the control unit is respectively connected with the incubator device (2), the culture dish device (3), the touch display device (4) and the interface unit (6).