CN113621625A

CN113621625A - Application of sesame SiERF103 gene in enhancing plant resistance

Info

Publication number: CN113621625A
Application number: CN202111094686.1A
Authority: CN
Inventors: 游均; 苏如奇; 刘爱丽; 黎冬华; 张秀荣
Original assignee: Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
Current assignee: Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
Priority date: 2021-09-17
Filing date: 2021-09-17
Publication date: 2021-11-09
Anticipated expiration: 2041-09-17
Also published as: CN113621625B

Abstract

The invention discloses the application of sesame SiERF103 gene in enhancing plant resistance, belonging to the technical field of plant genetic engineering, wherein the nucleotide sequence of the sesame SiERF103 gene is shown in SEQ ID NO. damage and/or drought resistance. The present invention reports for the first time the application of the sesame SiERF103 gene in enhancing plant waterlogging resistance and/or drought resistance. By constructing the SiERF103 gene overexpression vector, overexpressing it in Arabidopsis thaliana, and carrying out drought or waterlogging stress treatment, the study found that the restoration of normal growth conditions after drought or waterlogging stress treatment can restore a large number of transgenic Arabidopsis thaliana. normal growth. That is, the present invention confirms that the sesame SiERF103 gene has the function of improving plant waterlogging and drought resistance. This provides genetic resources for the genetic improvement of crop drought resistance and waterlogging tolerance, and is of great significance for promoting stable crop yields.

Description

Application of sesame SiERF103 gene in enhancing plant resistance

Technical Field

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of sesame SiERF103 gene in enhancing plant resistance.

Background

Sesame (Sesamum indicum L.) is a characteristic oil crop in China, has long cultivation history, is spread all over the country, is planted in the world, is a main producing country and a trade country, and has insufficient self-sufficiency. In recent years, the yield of the sesame in China is seriously reduced, which is greatly related to the influence of various abiotic stresses such as drought, waterlogging and the like on the sesame in the growth and development process. The sesame industry technical system has been used for investigating and analyzing most sesame main producing areas in China, and the sesame is affected by waterlogging and drought stress all the year round in China, so that the growth and development of the sesame are seriously affected, and the sesame is an important reason for reducing the yield of the sesame. Therefore, the drought resistance and the waterlogging resistance of the sesame are improved, the high and stable yield is promoted, and the method is particularly important for promoting the production development of the sesame in China and solving the problem of insufficient total amount of self-supply. At present, researches on the drought resistance and stain resistance related gene functions of sesame are deficient, and molecular related research reports are relatively few, so that the development of sesame stress resistance functional genes has important significance for developing sesame stress resistance molecular breeding and improving sesame stress resistance.

When stress occurs, the plant can quickly activate a large number of stress response genes and synthesize various functional proteins, and a series of complex signal networks are formed to resist adverse environments and maintain the normal physiological function of the plant, so that the aim of avoiding stress injury is fulfilled. The transcription factor is a protein molecule which can be specifically combined with cis-acting elements in the eukaryotic gene promoter region, thereby ensuring that a target gene is expressed at a specific strength and a specific time and space, and playing an important role in plant stress resistance. Such as arabidopsis thaliana RAP2.2 resists hypoxia stress by regulating the expression of hypoxia stress related genes such as ADH1, PDC1, and the like, carbohydrate metabolism, and ethylene synthesis related genes. Under the flooding condition, the rice Sub1A-1 inhibits the generation of ethylene and the transcription of growth and metabolism related genes, reduces the decomposition of starch and sugar, and ensures that the plant body survives in a low metabolism state. Sesame is used as an important oil crop, and the separation and identification of the stress-resistant gene of the sesame has very important significance for breeding new stress-resistant sesame varieties.

Disclosure of Invention

The invention provides the application of the sesame SiERF103 gene in enhancing the plant resistance aiming at the blank of the prior art, and the sesame SiERF103 gene is proved to have the function of enhancing the plant waterlogging resistance and the drought resistance for the first time through the over-expression experiment of the SiERF103 gene in the model plant transgenic arabidopsis thaliana, so that the gene resource is provided for the plant drought resistance and the stain-resistant breeding.

One of the purposes of the invention is to provide application of a sesame SieRF103 gene in enhancing plant resistance, wherein the nucleotide sequence of the sesame SieRF103 gene is shown in a sequence table SEQ ID NO.1, and the resistance comprises: resistance to waterlogging and/or drought.

Furthermore, the amino acid sequence of the protein coded by the sesame SieRF103 gene is shown in a sequence table SEQ ID NO. 2.

Further, a primer sequence for amplifying the sesame SieRF103 gene is shown in a sequence table SEQ ID NO. 3-4.

The invention also aims to provide an application of a vector and/or a cell containing the sesame SieRF103 gene in enhancing plant stain resistance and/or drought resistance.

Further, the vector containing the sesame SiERF103 gene is a cloning vector or an overexpression vector.

Furthermore, the overexpression vector is a vector pCAMBIA1301S-SiERF103 which is constructed by connecting sesame SiERF103 gene with a plant expression vector pCAMBIA 1301S.

Further, the construction method of the cell containing sesame SiERF103 gene comprises the following steps: amplifying to obtain sesame SiERF103 gene shown in SEQ ID NO.1, performing double enzyme digestion to obtain a linearized expression vector, connecting the sesame SiERF103 gene with the linearized vector, transferring the linearized vector into a receptor cell, and culturing, screening and identifying to obtain the sesame SiERF103 gene.

The invention also aims to provide the application of the sesame SieRF103 gene, or the protein coded by the sesame SieRF103 gene, or the vector and/or the cell containing the sesame SieRF103 gene in enhancing the stain resistance and/or drought resistance of plants.

The fourth purpose of the invention is to provide a method for improving the waterlogging and/or drought resistance of plants, which comprises the following steps: transferring an overexpression vector containing sesame SiERF103 gene into agrobacterium-infected cells, and infecting plants to obtain plants with improved stain damage and drought resistance.

Further, the plants with increased waterlogging and drought resistance can resume normal growth after being subjected to drought stress and/or waterlogging stress.

Compared with the prior art, the invention has the beneficial effects that: the invention reports the application of sesame SiERF103 gene in enhancing plant resistance for the first time, in particular to enhancing plant waterlogging resistance and/or drought resistance. Through constructing an SiERF103 gene overexpression vector, the vector is overexpressed in a model plant Arabidopsis thaliana, and drought or waterlogging stress treatment is carried out, researches show that the normal growth condition is recovered after the drought or waterlogging stress treatment, and a large amount of transgenic Arabidopsis thaliana can be recovered to grow normally. The invention proves that the sesame SiERF103 gene has the function of improving plant waterlogging and drought resistance. The gene provides gene resources for drought resistance and stain resistance genetic improvement of crops, and has important significance for promoting stable yield of crops.

Drawings

FIG. 1 is the construction of plant expression vector pCAMBIA1301S-SiERF103 containing SiERF103 gene in example 1 of the present invention;

FIG. 2 shows the result of the fluorescent quantitative PCR detection of transgenic SiERF103 Arabidopsis T1 generation plants in example 2 of the present invention;

FIG. 3 shows the drought resistance assay results of SiERF103 transgenic Arabidopsis thaliana in example 2 of the present invention, wherein FIG. 3-A shows phenotypes of SiERF103 transgenic Arabidopsis thaliana and wild type Arabidopsis thaliana that resume growth after normal growth and drought stress; FIG. 3-B is the survival rate of SiERF103 transgenic Arabidopsis thaliana and wild type Arabidopsis thaliana after drought stress;

FIG. 4 shows the measurement results of stain tolerance of SiERF103 transgenic Arabidopsis thaliana in example 2 of the present invention, wherein FIG. 4-A shows phenotypes of SiERF103 transgenic Arabidopsis thaliana and wild type Arabidopsis thaliana, which resume growth after normal growth and stain stress; FIG. 4-B shows the survival rates of SiERF103 transgenic Arabidopsis thaliana and wild Arabidopsis thaliana after waterlogging stress.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The method for cloning the drought-resistance-related gene SiERF103 described in the present invention is a method commonly used in the art. There are also a variety of well-established techniques for extracting mRNA, and a kit (EASY spin Plus Plant RNA kit) is available commercially (from Aidlab Biotechnologies Co., Ltd.). The methods of enzyme digestion, ligation, inflorescence infection and the like used for constructing the vector and transferring the vector into a plant are also common techniques in the field. The plasmids involved therein (pCAMBIA1301), media for transfection (Agrobacterium tumefaciens LBA4404 and reagent components used such as sucrose, Kan, hygromycin, etc.) are commercially available.

Example 1 obtaining of sesame SieRF103 Gene and construction of overexpression vector

1. Acquisition of sesame SieRF103 Gene

114 sesame ERF genes including SiERF103 gene (gene accession number: LOC105175593) are identified and obtained in NCBI database by utilizing protein sequence of Arabidopsis ERF gene and through homology sequence alignment analysis.

To analyze the function of the SiERF103 gene in stress resistance, primers were designed that amplify its complete CDS sequence based on the CDS sequence of the SiERF103 gene. Taking root tissues of stress-resistant sesame varieties with 75 strains in the initial flowering period and 7 days later, extracting total RNA by using an EASYspin Plus plant RNA extraction kit (purchased from Aidlab), and carrying out reverse transcription on the total RNA by using reverse transcriptase (purchased from Vazyme) to synthesize cDNA (the method is operated according to the instruction of a reverse transcriptase reagent of Vazyme). And performing conventional PCR amplification by using the designed primer by using the reverse transcribed cDNA as a template. The name and sequence (including modified base) of the designed primer are as follows:

SiERF103FL-F:

AGCTTTCGCGAGCTCGGTACCATGAGAATGATTCTCAAGAAAT (shown as SEQ ID NO. 3)

SiERF103FL-R:

CAGGTCGACTCTAGAGGATCCTCAAACCATCTCACTTGACACACTAC (shown in SEQ ID NO. 4)

And (2) amplifying a cDNA fragment containing the complete coding region of the SiERF103 gene by using the primer, and sequencing the amplified fragment to obtain the nucleotide sequence of the SiERF103 gene, wherein the nucleotide sequence is shown as SEQ ID NO. 1. The amino acid sequence of the protein coded by the protein is shown as SEQ ID NO. 2.

2. Construction of overexpression vector containing sesame SieRF103 gene

The sesame SieRF103 gene obtained by amplification is connected with pCAMBIA1301S (provided by the laboratory) plasmid by utilizing a homologous recombination method to construct a plant expression vector, which is named as pCAMBIA1301S-SieRF103 (the map is shown in figure 1), and the specific operation is as follows:

firstly, a linearized vector is obtained by a double enzyme digestion (KpnI and BamHI) (Takara) method, and the linearized vector with high purity is obtained by agarose gel electrophoresis and purification with a gel recovery kit (Tiangen Biochemical technology Co., Ltd.).

Adding the target fragment DNA and the linearized vector into a 1.5ml centrifuge tube according to the molar ratio of 3:1 for recombination reaction, uniformly mixing, placing in 37 ℃ water bath for about 30min, adding 10 mu L of reaction liquid into 50 mu L DH5a competent cells which are taken out from a-80 ℃ refrigerator and melted on ice for 10min, lightly mixing by a pipettor, then incubating on ice for 20min, thermally shocking in 42 ℃ water bath for 45 s, and rapidly placing on ice for cooling for 2 min.

③ 300 microliter LB liquid culture medium is added into the clean bench and incubated for 60min at 37 ℃. Centrifuging at 13,000rpm for 1min to collect thallus, discarding part of supernatant, re-suspending thallus with the rest culture medium, lightly spreading on LB solid culture medium containing Kan resistance with sterile spreading rod, and culturing at 37 deg.C for 16-24 hr by inversion in incubator.

Selecting several clones transformed by recombination reaction to carry out colony PCR identification, then respectively selecting each single colony identified as positive in a liquid LB culture medium containing Kan antibiotics at 37 ℃ in a 200rpm incubator for overnight culture, extracting plasmids or directly sequencing bacterial liquid, and identifying the carrier accuracy through enzyme digestion electrophoresis.

Example 2 application of sesame SieRF103 Gene in enhancement of plant stain damage and drought resistance

1. Obtaining of SiERF103 transgenic Arabidopsis material

(1) Recombinant vector transferred to agrobacterium LBA4404

Adding 2 mu g of plasmid DNA into every 100 mu L of LBA4404 agrobacterium-infected cells, lightly dialing the tube bottom by hands, uniformly mixing, and standing on ice for 5min, liquid nitrogen for 5min, water bath at 37 ℃ for 5min and ice bath for 5min in sequence.

② adding 700 mu L of LB liquid culture medium without antibiotics, and shaking-culturing for 5h at 28 ℃. The cells were collected by centrifugation at 13,000rpm for 1min, and 100. mu.L of the supernatant was collected, and the resuspended cells were gently blown and spread on LB solid medium containing Kan and Rif, and cultured in an inverted incubator at 28 ℃ for 2 days, and several positive clones were picked up, and colony PCR was performed using the primer SiERF103FL-F/R in example 1, to verify that the vector pCAMBIA1301S-SiERF103 had been transferred to Agrobacterium tumefaciens LBA 4404.

(2) Cultivation of Arabidopsis thaliana

Firstly, counting a certain amount of arabidopsis seeds according to the experimental requirement, and filling the seeds into a sterile 1.5mL centrifuge tube.

② adding 1mL of 75 percent ethanol, reversing the upside down and mixing evenly, abandoning the supernatant and repeating for 1 time. Placing into a shaker at 37 deg.C and 200rpm, and shaking for 10min for surface sterilization.

③ abandoning 75 percent ethanol, adding 1mL 95 percent ethanol, reversing the mixture up and down, mixing the mixture evenly, abandoning the supernatant, and repeating the process for 1 time. In a clean bench, 300-.

Fourthly, after the ethanol is volatilized, dibbling the dried arabidopsis seeds on a plate of the prepared MS culture medium by using toothpicks.

Sealing the flat plate, performing vernalization for 48h at 4 ℃ under the dark condition, after vernalization is finished, vertically culturing the flat plate in an illumination incubator, and transplanting after seedling emergence for one week.

Sixthly, the seedlings are planted in soil of a small pot by using tweezers, are firstly moisturized by using a preservative film for 48 hours, and are placed in a plant growing room for culturing until the growth of arabidopsis is bolting (about one month) for a transformation experiment.

(3) Genetic transformation

Activating agrobacterium: 20 mu.L of Rif and Kan are added into 20mL of LB liquid culture medium respectively, shaken and inoculated, and activated by shaking at 220rpm at 28 ℃ for 8-10 h.

② agrobacterium expansion culture: respectively adding 200 mul of Rif and Kan into 200mL of LB liquid culture medium, adding 5-10mL of activated bacterium liquid, shake culturing at 28 ℃ and 220rpm for 14-16h until OD value is 1.6-2.0, centrifuging at 4500rpm for 10min, removing supernatant from the precipitated thallus, and naturally drying.

And thirdly, adding 100mL of 5% sucrose solution into the precipitated bacteria to resuspend the bacteria, and blowing and beating the bacteria uniformly by a pipette to resuspend the bacteria.

Fourthly, adding the bacterial liquid in the centrifuge bottle into a plate, then adding 100mL of 5% sucrose solution, adding 40 mu L of Silwet-L-77 (0.02%) before conversion, and shaking the plate for uniform mixing.

Fifthly, folding the arabidopsis inflorescence, immersing the arabidopsis inflorescence into a flat dish, and slightly shaking for 15 s.

Sixthly, the plant is sleeved by a black bag, and is protected from light and moisture for 24 hours.

Seventhly, the transformation is repeated once again after one week.

(4) Screening of T1 positive plants and fluorescent quantitative PCR detection

Planting seeds harvested from T0 generation of Arabidopsis thaliana, sterilizing, inoculating an MS screening culture medium containing 30mg/L hygromycin (adding 25mg/L of cefamycin for bacteriostasis) and culturing for 7-10 days at 22 ℃ under illumination, screening to obtain positive plants (plants with normal growth of seedlings and roots), transplanting the positive plants into soil, covering the soil with a preservative film for 2-3 days, then uncovering the film, and then growing normally.

Taking young leaves of a positive transgenic plant and a wild type arabidopsis thaliana plant, extracting total RNA of the leaves by using an RNA extraction kit (Beijing Edrley Biotech Co., Ltd.), then obtaining cDNA by using a reverse transcription kit (Nanjing NuoWei Zan Biotech Co., Ltd.), taking respective cDNA as templates, and taking arabidopsis thaliana beta-actin as an internal reference, wherein a primer of the beta-actin gene comprises:

actin-F: 5'-CCCGCTATGTATGTCGCCA-3' (shown in SEQ ID NO. 7);

actin-R: 5'-AACCCTCGTAGATTGGCACAG-3' (shown in SEQ ID NO. 8);

the SiERF103 specific primer is used for SiERF103 QRT-F/R:

SiERF103 QRT-F: 5'-GAACTACAGAGGCGTGAG-3' (shown in SEQ ID NO. 5);

SiERF103 QRT-R: 5'-TTATCGTAAGCCAAAGC-3' (shown in SEQ ID NO. 6).

qRT-PCR expression verification of target gene (qRT-PCR Mix: Nanjing Nodezam Biotech Co., Ltd.; Instrument: Roche)

480)。

The qRT-PCR detection result is shown in figure 2, and the result shows that the expression quantity of the SiERF103 gene in 3 detected transgenic arabidopsis thaliana strains (SiERF103-OE #1, -OE #2, -OE #3) is obviously improved by taking a wild type plant as a control.

And (3) carrying out single plant harvest on transgenic T1 generation plants with overexpression of SiERF103, and continuously carrying out hygromycin screening on seeds harvested from T1 generation positive plants to obtain T2 generation positive plants and carrying out single plant harvest. Propagation is continued until homozygous SiERF103 overexpressing transgenic material is obtained.

2. Drought resistance assay for transgenic Arabidopsis thaliana

When the homozygous transgenic arabidopsis thaliana of the T3 generation grows to 3 pairs of leaves, drought stress (i.e. no watering) treatment is respectively carried out on the transgenic arabidopsis thaliana plant and the wild arabidopsis thaliana plant for 14 days, then rehydration recovery growth is carried out for 7 days, the number of plants recovering normal growth in each group is observed and counted, and the survival rate is calculated. Wherein the transgenic line and the wild type material are respectively subjected to 3 times of repeated experiments, and each repeated experiment is used for carrying out stress treatment on 27 plants.

The drought resistance determination results are shown in fig. 3, and the results show that only 5-7 wild arabidopsis plants are recovered to be normal after rehydration, 23-26 transgenic materials are recovered, and about 90% of arabidopsis thaliana strains transformed with the SiERF103 gene are calculated to be recovered to be normal in growth, and only about 22% of wild arabidopsis thaliana plants are survived. The result shows that the over-expression of the sesame SieRF103 gene can obviously improve the drought resistance of plants.

3. Measurement of stain resistance of transgenic Arabidopsis thaliana

When the homozygous transgenic arabidopsis thaliana of the T3 generation grows to 3 pairs of leaves, respectively carrying out waterlogging stress treatment on the transgenic arabidopsis thaliana plant and the wild arabidopsis thaliana plant for 18 days, wherein the waterlogging stress treatment specifically comprises the following steps: placing the box for planting plants in water, wherein the water is 0.5cm higher than the soil surface, and ensuring that the roots of the plants are immersed in the water; normal growth was then resumed for 7 days. And observing and counting the number of the plants which recover normal growth in each group, and calculating the survival rate. Wherein the transgenic line and the wild type material are respectively subjected to 3 times of repeated experiments, and each repeated experiment is used for carrying out stress treatment on 27 plants.

The results of the stain resistance measurement are shown in FIG. 4, and show that 6-9 strains of wild type and 18-24 strains of transgenic material were recovered 7 days after the normal treatment. Through calculation, over 75% of SiERF103 overexpression transgenic strains gradually recover to normal growth, and only about 25% of wild arabidopsis thaliana recovers to normal, which shows that the over-expression of sesame SiERF103 genes can improve the stain resistance of plants.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.

Sequence listing

<110> institute of oil crop of academy of agricultural sciences of China

Application of <120> sesame SieRF103 gene in enhancing plant resistance

<160> 8

<170> SIPOSequenceListing 1.0

<210> 1

<211> 435

<212> DNA

<213> sesame (Sesamum indicum L.)

<400> 1

atgagaatga ttctcaagaa atcgtattac accaactcct caacgaggcc tgatgcctta 60

aacacttctt cgcatcagat caggagtcag agttttaacc tccgcccgtc tcattccgtc 120

acaaagaaga actacagagg cgtgaggcgg cggccctggg ggaagttcgc agccgagatt 180

cgggactcca accggcaggg ggcaagaata tggctgggga cattcgaaac agccgaagaa 240

gctgctttgg cttacgataa ggcggcattt agaatgcgag gcacaaaggc tctcctcaat 300

ttccctcctg aaatcgtggc tgctgctgct tcttcatcga gcactccaac acttgatcgc 360

gatcaaaaac atttggataa ttctagaaac tgtttgtctg atgatgcaag tagtgtgtca 420

agtgagatgg tttga 435

<210> 2

<211> 144

<212> PRT

<213> sesame (Sesamum indicum L.)

<400> 2

Met Arg Met Ile Leu Lys Lys Ser Tyr Tyr Thr Asn Ser Ser Thr Arg

1 5 10 15

Pro Asp Ala Leu Asn Thr Ser Ser His Gln Ile Arg Ser Gln Ser Phe

20 25 30

Asn Leu Arg Pro Ser His Ser Val Thr Lys Lys Asn Tyr Arg Gly Val

35 40 45

Arg Arg Arg Pro Trp Gly Lys Phe Ala Ala Glu Ile Arg Asp Ser Asn

50 55 60

Arg Gln Gly Ala Arg Ile Trp Leu Gly Thr Phe Glu Thr Ala Glu Glu

65 70 75 80

Ala Ala Leu Ala Tyr Asp Lys Ala Ala Phe Arg Met Arg Gly Thr Lys

85 90 95

Ala Leu Leu Asn Phe Pro Pro Glu Ile Val Ala Ala Ala Ala Ser Ser

100 105 110

Ser Ser Thr Pro Thr Leu Asp Arg Asp Gln Lys His Leu Asp Asn Ser

115 120 125

Arg Asn Cys Leu Ser Asp Asp Ala Ser Ser Val Ser Ser Glu Met Val

130 135 140

<210> 3

<211> 43

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 3

agctttcgcg agctcggtac catgagaatg attctcaaga aat 43

<210> 4

<211> 47

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 4

caggtcgact ctagaggatc ctcaaaccat ctcacttgac acactac 47

<210> 5

<211> 18

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 5

gaactacaga ggcgtgag 18

<210> 6

<211> 17

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 6

ttatcgtaag ccaaagc 17

<210> 7

<211> 19

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 7

cccgctatgt atgtcgcca 19

<210> 8

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 8

aaccctcgta gattggcaca g 21

Claims

1. the application of sesame SiERF103 gene in enhancing plant resistance, it is characterized in that, the nucleotide sequence of described sesame SiERF103 gene is as shown in sequence listing SEQ ID NO.1, and described resistance comprises: waterlogging resistance and/ or drought resistance.

2. application according to claim 1, is characterized in that, the aminoacid sequence of the protein encoded by described sesame SiERF103 gene is as shown in sequence table SEQ ID NO.2.

3. application according to claim 1, is characterized in that, the primer sequence for amplifying described sesame SiERF103 gene is as shown in sequence table SEQ ID NO.3-4.

4. The application of a vector and/or cell comprising the sesame SiERF103 gene as claimed in claim 1 in enhancing plant waterlogging resistance and/or drought resistance.

5. application according to claim 4, is characterized in that, described carrier that comprises sesame SiERF103 gene is cloning vector or overexpression vector.

The application according to claim 5, wherein the overexpression vector is the vector pCAMBIA1301S-SiERF103 constructed by linking the sesame SiERF103 gene and the plant expression vector pCAMBIA1301S.

7. application according to claim 4 is characterized in that, the construction method of the described cell comprising sesame SiERF103 gene is: amplification obtains the sesame SiERF103 gene as shown in SEQ ID NO.1, and double enzyme cut obtains linearization The expression vector is obtained by connecting the sesame SiERF103 gene with the linearized vector and transferring it into recipient cells, and obtaining after culture screening and identification.

8. the sesame SiERF103 gene as described in claim 1, or the albumen as described in claim 2, or the carrier and/or the cell comprising the sesame SiERF103 gene as described in claim 4 in enhancing plant waterlogging tolerance and/or drought resistance applications.

9. a method for improving plant waterlogging and/or drought resistance, is characterized in that, described method comprises: the overexpression vector comprising sesame SiERF103 gene is transferred in Agrobacterium competent cell, and infects plant, obtains Plants with increased waterlogging and drought resistance.

10 . The method according to claim 9 , wherein the plants with improved waterlogging and drought resistance can resume normal growth after being treated by drought stress and/or waterlogging stress. 11 .