CN113414049B - Intelligent atomizer and using method thereof - Google Patents

Abstract

An intelligent atomizer and a method for using the same, relating to the technical field of intelligent atomizers; including the following operation steps: S1: collecting and pre-processing environmental particulate matter; S2: atomizing treatment; S3: inactivating blue light; S4: releasing. In the prior art, the intelligent atomizer to kill viruses is not satisfied with the current demand. In the present invention, the wave band of 450nm is selected as the radiation source to reduce the potential unsafe factors that may be brought by light, regulate the radiation intensity, retain and improve the immunogenic performance of the irradiated substance, and select the pretreatment method of vitamin B2 sensitization, which is specific The structure in the nucleus of potential pathogens is selectively irradiated to inactivate and lose infectivity, and then the outer membrane structure and the glycoprotein embedded in the membrane are preserved as much as possible by reducing the unit irradiation intensity Integrity of other antigenic macromolecules.

Description

A kind of intelligent atomizer and using method thereof

technical field

The present invention relates to the technical field of intelligent atomizers, more specifically, the present invention relates to an intelligent atomizer and its usage method.

Background technique

Pathogens transmitted by the respiratory tract, such as respiratory adenovirus transmitted between humans in spring and autumn, swine fever virus transmitted among animals, Mycoplasma hyopneumoniae, influenza transmitted between humans and animals, coronavirus and other pathogens, enter the respiratory epithelial tissue of animals under the condition of intact vitality , Often cause a series of contact respiratory infectious diseases, and spread rapidly, and the incidence rate is often high. One of the more cost-effective ways to prevent and control this type of disease is to use spray immunization.

It has been reported in the literature that the method of spraying to prevent swine fever has achieved new results. The method of spraying is used to prevent swine fever with the generated aerosol. The technical route originated from the nasal spray immunization of SPF chickens to obtain specific antibodies for the laboratory to obtain antibody reagents quickly and conveniently. It has been further developed into a more practical use of this technology for large-scale immunization of large, medium and small animals, thus changing the traditional method of capturing animals, immobilizing them, and requiring individual intramuscular or subcutaneous injections. It fundamentally solves the practical problems of being tedious, labor-intensive, time-consuming and inefficient. According to related reports, this kind of work was tested on 3,500 fattening pigs with the attenuated swine fever vaccine produced in China at two independent pastures in Jiangsu, China, and a satisfactory preventive effect was obtained. The method is to close the piggery first and use it as an aerosol chamber, and use a special aerosol generator to spray the vaccine for about 1 hour. The results confirmed that no adverse health effects were found, nor were any damage to the respiratory system of the pigs noted. Studies have shown that this spray immunization has good sensitivity and specificity. A durable and stable immunity can be produced 78 days after spraying with this attenuated classical swine fever vaccine, and good protection can be maintained for not less than 10 months.

For example, the commercially available attenuated Mycoplasma hyopneumoniae vaccine has a better immune effect on hybrid pigs, but the immune route of intrapulmonary injection of the vaccine has affected its widespread promotion. Some organizations have tried the method of aerosol immunization for large-scale prevention and control, and it has also been proved that this method is a convenient and effective way of immunization, especially the spray immunization of attenuated or attenuated vaccines against respiratory pathogens, which is more effective. play a direct and effective role. It is also found that the vitality of the attenuated vaccine whose virulence has been reduced to a certain extent will directly affect the immune protection effect of the vaccine. What often needs to be considered is that the shear force generated by the spray system during the atomization process will have a certain impact on the microbiological activity of the vaccine, and even reduce the titer by 1. The attenuated strain of Mycoplasma hyopneumoniae can still be isolated, cultivated, amplified, and strengthened in the laboratory. Therefore, if you consider the principle of safety first, it is best to choose a completely inactivated vaccine. Although the immune effect of the attenuated and attenuated vaccine is stronger than that of the inactivated vaccine, it can fully stimulate the cellular immunity and humoral immunity of animals. However, if it is used for large-scale population spray immunization, or to deal with uncertain biological background, or to deal with unknown new pathogens and their virulent mutant strains, etc., it is hoped that it can be achieved at the earliest initial stage Obtaining the safest and earliest "natural immunity" prevention and control benefits, and adopting the safest "inactivated virus" method are the principles that should be given top priority. Even if you don’t want to sacrifice some of the immunostimulatory efficacy, you need to consider the choice after first determining the biological background of the pathogen.

This application proposes that the pathogen particles present in the air are pretreated by hydration and sensitized, then inactivated by atomization and blue light irradiation, and then released back to the atmosphere. This treatment method firstly ensures that the pathogen particles in the air are After being inactivated, the safety of the human body or the immune target is guaranteed first, and then the immunogen can play the role of immune stimulation. The process designed in this way not only guarantees safety, but also plays a pre-preventive and protective role especially for unknown potential pathogens.

Compared with the intelligent atomizer method used for spray immunization of the present application: The spray equipment of the spray immunization for the economic animal immunization of the report focuses on the control of the particle size of spray particles or aerosol, and the purpose is to as far as possible To maximize the respiratory tract's acceptance of the immunogen, for example, aerosol particles with a size of 10um are a more preferred one. In these occasions, it is emphasized to avoid damage to the pathogen, especially the nucleic acid structure, by high-frequency and high-power ultrasonic methods, and to prevent excessive loss of the activity of the immune vaccine placed in the smart nebulizer.

Due to the global spread of the new crown epidemic, how to prevent and control respiratory viruses has attracted much attention recently. Nausheen R.Shah of Maine State University released and demonstrated his own design prototype (N.R.Shah, I.Masic, C.Jonesand R.Gupta, Personal ultraviolet respiratory germ eliminating machine(purgem) for covid-19.WSU-PHY- 2001, 2002), the purpose is to provide a simple, cheap, easy to popularize and use, and safe and effective virus inactivation technology and device in the air. Inventor Shah has verified and quoted other researchers’ experimental conditions that 220nm, 222nm, 245nm, 254nm, 260nm, 265nm and other deep ultraviolet band LEDs have the efficiency in inactivating viruses, and the output radiation power distribution is less than 2mJ /cm2, 3.7mJ/cm2, 40mJ/cm2, 50mJ/cm2 range. In particular, the applied for patent document emphasizes that the inventive use of the device is for the purpose of single-person air filtration and connection with various air treatment systems, so it is particularly concerned about whether it will produce toxic By-products such as ozone, the inventor Shah has verified that no ozone is produced under 260nm irradiation, so it is considered safe. In order to improve the efficiency of irradiation, the inventor Shah further optimized the structure of the airway. The material of the airway is PTFE, a UV reflective material, which increases the irradiation efficiency by 10-50 times. The LED power supply uses a 12V battery that can provide a constant current source of 500mA Driven by air cooling and cooling fins to keep the LED working stable and long-lasting. The final virus inactivation ability can reach 99.99%, which can be understood as reducing the virus titer by 4 units.

In order to achieve a safer virus inactivation technology, a methylene blue or vitamin B2 inactivation technology is used clinically, adding as little as 1uM methylene blue and 10-200umol/L VB2 to the solution, then The ability to target virus inactivation can be achieved. Even more fragile cultured cells can be used. For example, adding riboflavin combined with 365nm ultraviolet irradiation and normal cultured cervical cancer cells with only 365nm ultraviolet irradiation, there will be significant differences in the morphological changes of the cells. When the concentration of Vb2 is low, riboflavin is mainly concentrated in the nuclear membrane When the concentration is high, it is also distributed on the cell membrane, and the photolysis of riboflavin under light conditions can significantly damage the surrounding microstructure. Compared with simple ultraviolet irradiation without adding Vb2, the targeting properties are very obvious, and the required irradiation dose is also much lower.

Compared with methylene blue, riboflavin (vitamin B2) is a natural coenzyme with a wide range of functions. It can be used as a photosensitizer here, together with FMN, FAD, riboflavin and derivative compounds to participate in various redox processes in vivo . Riboflavin, FMN and FAD are easily degraded under the irradiation of light sources with wavelengths of 220nm, 267nm, 370nm, 420nm to 560nm. What is surprising is that riboflavin has the highest quantum efficiency for blue light with longer wavelengths. Flavin can generate active oxygen molecules, including superoxide anion and singlet oxygen, to break the guanine in nucleotides, and break the DNA superhelical structure of bacteria or viruses into open rings or lines. At present, the photochemical technology of UV irradiating riboflavin has become mature, which has become an effective and safe technology to kill microorganisms. On the contrary, the 450nm light source is actually used as a radiation source for plant growth. For medical purposes, the radiation inactivation of light sources near 450nm is mainly reported for non-contact and non-destructive processing of plasma or whole blood products.

Compared with existing or described smart nebulizers, for example, household smart nebulizers or humidifiers, as well as traditional medical smart nebulizers or humidifiers, generally retain the function of sterilizing and inactivating viruses. The wide-spectrum broad-spectrum ultraviolet device is either directly irradiated for physical damage, or sterilized or inactivated by means of free radicals with oxidation characteristics generated by metal catalysts. Or use electrostatic adsorption to settle suspended particles in the air. If there is a special pursuit of killing pathogens, the energy is generally increased by increasing the power of the irradiation or using an irradiation source with a shorter wavelength as much as possible. It does not adopt the method similar to this application, through optimizing and combining the interaction mechanism of comprehensive factors, to achieve a more specific solution that is beneficial to obtaining social security, or to draw a conclusion after actual testing and screening which wavelength selection is more technical Advantages on the subject or advantages that are compatible with other requirements.

Contents of the invention

The invention aims to solve the technical problem that the intelligent atomizer in the prior art does not meet the current demand for killing viruses.

Purpose and effect of the present invention are achieved by the following specific technical means:

A method for using an intelligent atomizer, comprising the following steps:

S1: Collect and process environmental particles: use air supply equipment to capture bacteria, mycoplasma, virus particles, etc. that naturally exist in the air or artificially produced pathogen particles, aerosols, etc., to particles containing 10-200umol/L vitamin B2 in aqueous solution;

S2: Atomization treatment: In the fogging unit of the device, these pretreated pathogen particles are dispersed into tiny droplets, which are further shaped and recovered after peeling off the hydration layer;

S3: Blue light inactivation: the droplet enters the 420-460nm blue light irradiation cavity in the form of 1-100um in size and stays for 1-20 seconds to receive the irradiation of 1.0-4000mJ/Second from the irradiation source;

S4: Release: The irradiated pathogenic particles are released back into the gas phase of the environment, replacing the known or unknown virulent pathogens originally present in the environment to stimulate the body to produce a controlled immune response.

Preferably, the concentration of particles such as bacteria, mycoplasma, virus particles, etc. or artificially produced pathogen particles, aerosols, etc. in the air is first increased by 2-6 orders of magnitude in the liquid phase before post-processing, and from the irradiation chamber The concentration of particles released from the outlet is greater than that in the original air.

Preferably, the abundance of the 420-460nm band output by the blue light radiation source is not less than 50% of the total irradiated energy, and the blue light radiation source is embedded in a thermally conductive insulating glue together with its thermally conductive metal heat sink, including a constant voltage source and multiple parallel constant current branches composed of constant current ICs or PTC thermistor current limiting resistors.

Preferably, the air supply volume of the air supply device, the atomization volume of the fogging unit, and the parameters of the unit radiation energy of the blue light radiation source are simultaneously controlled by a PWM output signal control system through preset coefficients.

Preferably, the control parameters of the control system include: the air supply speed of the air supply equipment, the cumulative air-water mixing time, the ultrasonic spray speed, the cumulative light radiation energy per particle, and the concentration of riboflavin in the solution. The setting of these control parameters and their combinations is aimed at controlling the characteristics of the immunogenic activity of the released particles, especially for different types of pathogens, it is necessary to use optimized parameter combinations.

The invention provides an intelligent atomizer, which includes a water tank with a water inlet pipe, an air supply device, a fogging unit and a spray inactivation unit, an air distributor is installed inside the water tank with a water inlet pipe, and the air supply device includes a receiver The interface of the air source and the interface output to the air distributor. A fogging unit is installed on the upper end of the water tank. The fogging unit includes a liquid level track tube, an ultrasonic vibrator, a venturi tube and a droplet recovery sleeve with a funnel on the top. The liquid level track tube is equipped with an ultrasonic vibrator that moves with the change of the liquid level. The upper end of the liquid level track tube is located on the upper end of the ultrasonic vibrator. There is a spray inactivation unit on the top of the fogging unit, and a droplet recovery sheet is arranged between the fogging unit and the spray inactivation unit. The spray inactivation unit includes a fixed seat, a reflection tube, a quartz transmission tube and an LED array board. The upper end of the fixed seat There is a reflection tube, the upper end of the fixing seat is located inside the reflection tube and a quartz transmission tube is provided, and the lower end of the quartz transmission tube is connected to the fogging unit, and the outside of the quartz transmission tube is provided with an LED array board, and the radiation is between the LED array board and the quartz transmission tube. According to cavity.

Preferably, an integral heat dissipation substrate is provided on the outside of the LED array board.

Preferably, a square through groove is arranged in the center of the droplet recovery sheet, and a gas-liquid separation mechanism is arranged in the through groove. There are filter plate 1, filter plate 2, and filter plate 3. The gas-liquid separation apertures of filter plate 1, filter plate 2, and filter plate 3 decrease step by step, and the gas-liquid separation aperture of filter plate 3 is 20um.

Preferably, movable grooves are provided on the left and right sides of the corresponding filter plate 2 inside the through groove, the inner side of the movable groove is set as a smooth inclined surface, and the inclined surfaces inside the movable groove are arranged in parallel, and the left and right sides of the filter plate 2 are fixed with The lug plate is installed, and the side of the lug plate close to the inner side of the movable groove fits and connects with the inclined surface inside the movable groove.

Preferably, the filter plate 2 is provided with a cavity, and the upper and lower ends of the cavity are provided with several groups of filter tips extending to the outside of the filter plate 2, and the outside of the filter tips are respectively connected with the filter plate 1 and the filter plate 2, and are in the same row of the cavity. The filter tips are fixedly connected, and the corresponding side of the filter tip and the cavity is fixedly connected with a spring. When the filter plate is in the second initial position, the spring at the upper end of the cavity is in a normal state, and the spring at the lower end of the cavity is in an extended state. The filter tip includes an air intake end and a filter end, the air intake end and the filter end are integrally formed, the air intake end is provided with an air inlet, and filter screens are provided on both sides of the filter end.

Beneficial effect

1. Use the device manufactured by the present invention and the method of use provided to inactivate known or unknown virus particles in an optimized manner to obtain the safety of ambient air; wherein in an optional manner, the final inactivated virus The concentration of the particles is increased by 2-6 orders of magnitude to act as a natural immunogen and released back to the environment. Such a beneficial effect is that the nucleic acid structure of the passing pathogen particles is completely or partially destroyed, and the pathogen particles are finally eliminated. alive and re-released into the gas phase environment.

2. In the present invention, try to select the 450nm wave band in the visible light band as the radiation source, in order to further reduce the potential unsafe factors to the cornea of human eyes that blue light may bring, further reduce the radiation intensity, but at the same time, in order to improve the radiation intensity For biological effects, the pretreatment method of vitamin B2 sensitization is adopted, and the structure in the nucleus of potential pathogens is specifically used as the irradiation target to inactivate and lose infectivity, and then reduce the unit irradiation intensity. In this way, the integrity of the outer membrane structure and antigenic macromolecules such as glycoproteins embedded in the membrane should be preserved as much as possible.

3. Selecting 450nm LED as the light source can obtain significant advantages in terms of heat dissipation and photoelectric conversion efficiency, and can also greatly reduce the requirements for driving power. At the same time, selecting 450nm LED as the light source has economic advantages It's also obvious enough.

4. Open the gas trapping part in advance for 0.5-24 hours, and let it go through the cleaning of the aqueous solution in the water tank, which can concentrate the potential pathogenic particles suspended in the air by 2000-200000 times; then start the spraying and blue light inactivation part of the function , the titer of the inactivated pathogen particles will drop by 2-4 orders of magnitude.

Description of drawings

Fig. 1 is a schematic flow chart of the method for using the intelligent atomizer of the present invention.

Fig. 2 is a schematic cross-sectional front view of the intelligent atomizer of the present invention.

Fig. 3 is a schematic structural view of the water tank of the present invention.

Fig. 4 is a schematic diagram of the explosion of the fogging unit of the present invention.

Fig. 5 is a schematic top view of the spray inactivation unit of the present invention.

Fig. 6 is a schematic cross-sectional view of the LED array board of the present invention.

Fig. 7 is a front sectional schematic view of the droplet recovery sheet of the present invention.

FIG. 8 is an enlarged schematic diagram of A in FIG. 7 of the present invention.

Fig. 9 is a schematic structural view of the filter tip of the present invention.

In Fig. 1-9: water tank 1, air supply equipment 101, air distributor 102, water inlet pipe 103, liquid level track pipe 2, limit pipe groove 201, ultrasonic vibrator 3, venturi tube 4, droplet recovery sleeve 5 , funnel mouth 51, droplet recovery sheet 6, through groove 61, filter plate 1 62, filter plate 2 63, cavity 631, mounting ear plate 632, filter tip 633, adjustment hole 634, filter plate 3 64, movable groove 65, Water drop box 66, sliding rod 67, spray inactivation unit 7, fixing seat 8, reflection tube 9, quartz transmission tube 10, LED array board 11, LED patch chip 111, serial wire 112, positive cooling surface 113, negative cooling surface 114 , cover plate 12 , limit ear plate 13 , heat dissipation base plate 14 , irradiation chamber 15 , air inlet 16 , air inlet 161 , filter end 17 , and filter screen 171 .

Implementation

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

Referring to Figure 1, a method for using an intelligent atomizer includes the following steps:

S1: Collect and process environmental particles: use air supply equipment to capture and enrich rigid particles such as bacteria, mycoplasma, virus particles, etc. that naturally exist in the air or artificially produced pathogen particles, aerosols, etc. to a concentration of 10-200umol/ In the aqueous solution of L vitamin B2;

S2: Atomization treatment: In the fogging unit of the device, these pretreated pathogen particles are dispersed into tiny droplets, which are further shaped and recovered after peeling off the hydration layer;

S3: Blue light inactivation: the droplet enters the 420-460nm blue light irradiation cavity in the form of 1-100um in size and stays for 1-20 seconds to receive the irradiation of 1.0-4000mJ/Second from the blue light irradiation source, preferably 450nm blue light;

S4: Release: The irradiated droplets are released back into the gas phase of the environment, replacing the known or unknown virulent pathogens originally present in the environment, and stimulating the body to produce a controlled immune response.

Among them, the concentration of rigid particles such as bacteria, mycoplasma, virus particles, etc. or artificially manufactured pathogen particles, aerosols, etc. from the air is first increased by 2-6 orders of magnitude before processing, and the target finally released by the intelligent atomizer The ratio of the gas phase concentration of pathogenic particles to the concentration before treatment is greater than 2.0.

Among them, the abundance of the 420-460nm band output by the blue light radiation source is not less than 50% of the total irradiated energy, preferably the 450nm band. A constant voltage source and multiple parallel constant current branches composed of constant current ICs or PTC thermistor current limiting resistors.

Among them, the air supply volume of the air supply equipment, the atomization volume of the fogging unit, and the parameters of the unit radiation energy of the blue light radiation source are simultaneously controlled by a PWM output signal control system through preset coefficients.

Among them, the rate parameter control vectors for the correct operation of the control system include: the air supply speed of the air supply equipment, the cumulative air-water mixing time, the ultrasonic spray speed, the cumulative light radiation energy per particle, and the concentration of riboflavin in the solution.

Among them, the spray inactivation process and air mixing can be controlled separately. One of the preferred methods is to pre-air mixing to achieve the concentration of gas phase particles, and the pathogenic particles in the air are captured and enriched into the aqueous storage solution before spray inactivation with emissions.

Among them, the spray inactivation process and the air supply mixing can be coupled and carried out simultaneously. One of the preferred ways is to add the pathogenic samples to be treated into the water outlet tank and then spray, treat and discharge them.

Among them, the inactivation mode of a specific pathogen or a type of pathogen can be optimized by setting the rate parameter control vector.

Among them, the final traits of the pathogenic particles released by the smart atomizer are controlled by the rate parameter control vector, including particles that are attenuated but retain infectivity, and particles that are not infectious but retain immunogenicity.

Among them, the infectious properties of the pathogenic particles finally released by the smart atomizer include the option of complete loss and the option of partial retention.

Among them, the final option for the infectious traits of the released particles is the result of the optimization and combination setting of the control parameters.

A 40W home spray intelligent atomizer is placed in a room of 60 cubic meters to work. The air supply mode has a fast air supply mode and a spray air supply mode, and the LED array board 11 and the ultrasonic vibrator are also synchronously controlled. 3. The control mode of the specific power output is controlled by PWM continuous manual knob to ensure that the air supply volume of the fan is continuously adjustable from 0.1 to 50CFM. The 50um sintered air distributor 102 fully mixes the particles suspended in the air with the aqueous solution containing 50umol/L vitamin B2 for 30-60 minutes, then turns on the fogging unit and switches to the spray air supply mode, and the airflow pushes and wraps the active particles The aerosol or aerosol is irradiated in the blue light tube with a length of 18 cm, and the residence time in the blue light tube is about 2-4 seconds;

As shown in Figure 3, the 450nm LED array plate loaded in the blue light irradiation cavity 15, the continuously adjustable irradiation energy between 1mJ-1500mJ/cm2 is projected on the aerosol or aerosol particles in the tube through the quartz tube 10, Then it is reflected by the reflection tube 9. In the selection of other machine models, quartz, 10 can be replaced by LED special-shaped lamp boards embedded in insulating silica gel;

The LED array board 11 used in the home spray smart atomizer model is a combination of 30lm/1W 450nm, LED, and 3x3 chips on a 16mm aluminum substrate. Each column is preceded by a 350mA constant current IC or PTC thermal current limiting Resistor, together with the 12cm case fan, the DC power supply of this part comes from the same 12V/1.2A constant voltage output port, but the slight difference is that there is a separate manual switch responsible for controlling the LED array board to turn on and off Or automatically controlled by the selection of mode switching.

A set of industrial spray intelligent atomizers is installed in a 50x20m supermarket with a storey height of 3.5m, including a central control unit and 30 sets of spray inactivation units installed in a decentralized manner. The central control unit communicates with the spray inactivation units through the 485 serial port Communication, through the spray inactivation unit to output the inactivated fresh air power of 3KW, the air supply mode has night cruise air supply mode and silent spray air supply mode, in the night cruise air supply mode, the unit fan air supply volume is guaranteed to be 100-200CFM Working continuously for 6-10 hours, the fan quickly sucks the air in the environment and sends it into a 100L water tank 1. Through a group of 50um serially connected sintered air distributors 102, the particles suspended in the air are mixed with 10umol/L vitamin B2 The aqueous solution is fully mixed. After the silent spray air supply mode is turned on, the fogging unit switches to the spray air supply mode. The optional dry airflow to the shelf area and the wet airflow to the vegetable and flower area push the air wrapped with active particles. The mist or aerosol is irradiated to 3-6 groups of 18 cm long blue light tubes built in the unit, and the residence time in each blue light tube cavity is about 2-4 seconds, and the blue light LED in each spray inactivation unit 7 The array boards 11 all adopt the same electrical parameters, and are all embedded with insulating silica gel.

Chickens of all ages can be infected with Newcastle disease, young chicks are highly susceptible, and the mortality rate of non-immune chickens can reach more than 90%; the source of infection of Newcastle disease virus is mainly sick chickens, and the transmission routes are mainly respiratory system and Digestive canal and eggs; 50 chicks were randomly selected from a chicken coop in a private farm, and whole blood was collected from the underwing vein to make serum samples, and cloacal swabs were collected for Newcastle disease pathogen detection;

The hemagglutination and hemagglutination inhibition test procedures used in the antibody detection of serum samples are carried out according to the national standard GB/T16550-2008: 25ul of normal saline is added to each well of the first 12 wells of the 96-well hemagglutination plate; In the first well, take 25ul of the mixed virus solution from the first well and add it to the second well, and dilute the 11 wells sequentially in this way; change the tip, and add 25ul of normal saline to each well; change the pipette tip, Add 25ul, 1% red blood cell suspension to each of the 12 wells; place the reaction plate on a micro-oscillator to vibrate and mix well, and let it stand at room temperature (20-25°C) for 40 minutes to observe and judge the result; determine the concentration of the normal saline control well. The red blood cells have a typical button sinking result, and the reaction plate is further tilted to check the hemagglutination of the completely agglutinated virus fluid;

The specific process and operation of the hemagglutination inhibition test are as follows: add 25ul of normal saline to each well of 1-11 wells, add 25ul to the 12th well, absorb 25ul of the serum to be tested and place it in the first well, pipette 6-8 times to mix, and aspirate 25ul Mix well in the 2nd well, suck 25ul into the 3rd well, then doubly dilute to the 10th well, and aspirate 25ul from the 10th well and discard it, do not add the serum to be tested in the 11th and 12th wells, the 11th well As the virus control and 12 wells as the dilution control, change the pipette tip, dilute the standard positive serum and standard negative serum in the same way, change the pipette tip, and use a micropipette to pipette 4 units of the diluted Add virus solution to wells 1-11, 25ul per well, tap the reaction plate to mix, and let it stand at room temperature for 30min; change the pipette tip, add 25ul and 1% red blood cell suspension to wells 1-12 respectively, Place the reaction plate on a micro shaker for 15-30s to mix well, and let it stand at room temperature for 40 minutes to determine the result.

Add 600ul of lysate to the virus pathogen detection sample, negative control and positive control respectively, invert and mix thoroughly, and let stand at room temperature for 3-5min; suck the liquid into the adsorption column, and centrifuge at 13000r/min for 30s; discard the liquid in the collection tube, add 600μL washing solution, centrifuge at 13000r/min for 30s; move the adsorption column to a new 1.5mL centrifuge tube, add 50μL washing solution to the center of the column; let it stand at room temperature for 1min, and centrifuge at 13000r/min for 30s to collect viral RNA; take 2ul viral RNA The extract was tested by RT-PCR according to the national standard method. The conditions used: 42°C 45min; PCR program: 94 3min °C; 94 30s °C, 5530s °C, 72 30s °C for a total of 35 cycles, and the reaction solution was collected for electrophoresis to observe the amplification band the appearance of

50 healthy chicks located in an independent chicken house were tested for Newcastle disease antibody and pathogen. The test results showed that the serum antibody level of 96% of the chicks was less than 5log2, and the virus RNA detection of cotton swabs were all negative;

According to the national standard method, extract the isotonic saline solution of the lung tissue, intestines, feathers and other tissues of 5 dead chickens infected with Newcastle disease from the Newcastle disease area, pre-filter, sterilize, filter and dehydrate by ultrafiltration to obtain 300ml concentrated virus-containing samples solution, divided into 3 parts of 100ml, and stored at -20°C;

Take out 1 part each time, add 0.9% normal saline containing 0.05% polyvinylpyrrolidone K30 buffer at a ratio of 1:20, and dilute to make a virus spray solution;

Place a small high-pressure aerosol generator in an empty chicken coop of about 100 square meters, and spray diluted virus spray liquid to the indoor space at a rate of 5-10ml per minute. At the same time, turn on a 100W industrial spray intelligent atomizer, select the concentration mode as the working method, adjust the air supply volume of the fan in the range of 2.0CFM to work continuously for 3-4 hours, and add 50umol/L vitamins to the 5L water storage tank 1 of the sprayer in advance B2, 30-60 minutes after the end of the spray, turn off the generator and the smart atomizer, move the smart atomizer to a separate chicken house to spray the healthy chicks, turn on the ultrasonic spray inactivation mode, and the spray speed is 200-500ml per hour, until Spray all the liquid in the 3L smart atomizer;

Repeat the above process every month, dilute 100ml of virus extract and spray the unavoidable chicks for 1 day; one month after spraying all 300ml of virus extract, detect the immunity of 50 chicks to Newcastle disease virus;

The result of the test was that 70% of the chickens were positive for serum antibodies, 15 samples had an antibody level less than 5log2, 14 samples had an antibody level of 5log2, 10 samples had an antibody level of 6log2, 7 samples had an antibody level equal to 7log2, and 3 samples had an antibody level of 8log2 , the antibody level of one sample was 9log2, and no samples greater than 9log2 were detected; the 362bp band of virus RNA detected by cotton swabs was all negative.

Referring to Figures 2-9, an intelligent atomizer includes a water tank 1 with a water inlet pipe 103, an air supply device 101, a fogging unit and a spray inactivation unit 7, and the inside of the water tank 1 with the water inlet pipe 103 is fixedly installed with The air distributor 102 and the air supply device 101 are fixed on the outside of the water tank 1. The air supply device 101 is a pressure fan. The air supply device 101 includes an interface for receiving the air source and an interface for outputting to the air distributor 102. The top of the water tank 1 is provided with a cover The plate 12 and the upper end of the water tank 1 are equipped with a fogging unit. The specific structure of the fogging unit is: a liquid level track tube 2, an ultrasonic vibrator 3, a venturi tube 4, and a droplet recovery sleeve 5 with a funnel opening 51 on the top. The liquid level track tube 2 is snapped and installed inside the water tank 1, and the limit pipe groove 201 is integrally formed around the outside of the liquid level track tube 2. The level ear plate 13 and the ultrasonic vibrator 3 are slidingly connected in the liquid level track tube 2. The ultrasonic vibrator 3 moves up and down with the change of the liquid level in the liquid level track tube 2. Venturi tube 4, the upper end of the venturi tube 4 is set with a droplet recovery sleeve 5 with a funnel mouth 51 on the top, and a spray inactivation unit 7 is arranged on the top of the fogging unit, and the gap between the fogging unit and the spray inactivation unit 7 A droplet recovery sheet 6 is provided, and the spray inactivation unit 7 includes a fixed seat 8, a reflection tube 9, a quartz transmission tube 10 and an LED array plate 11, and the outer side of the fixed seat 8 is fixed with a limit ear plate corresponding to the limit tube groove 201 13. The upper end of the fixing seat 8 is fixed with a reflection tube 9, the upper end of the fixing seat 8 is located inside the reflection tube 9 and a quartz transmission tube 10 is fixed, the lower end of the quartz transmission tube 10 communicates with the liquid level track tube 2, and the fixing seat 8 is located at the quartz transmission tube 10 An LED array board 11 is installed on the outside, and an irradiation chamber 15 is provided between the LED array board 11 and the quartz transmission tube 10; the air in the environment is sucked by a pressure fan, and the particles suspended in the air are passed through an air distributor 102 Fully mix with the aqueous solution containing vitamin B2 in the water tank 1, and then use the ultrasonic 3 vibrator to move the position of the liquid level track tube 2 up and down with the change of the liquid level, and make the aqueous solution into a liquid with a size of 1-100um. The air flow pushes through a venturi tube 4, and then through the droplet recovery sheet 6, and the excess droplets fall onto the droplet recovery sleeve 5 with a funnel mouth 51 on the top, and are fed into the water tank 1 through the funnel mouth 51. The recombined and smaller droplets wrap the suspended rigid particles and push them into the quartz transmission tube 10 in the spray inactivation unit 7 to receive 450nm radiation, and stay in the quartz transmission tube 10 for 1-10 minutes under the action of the airflow. Seconds later, it is re-released into the environment to suspend in the form of a stable aerosol, and the 450nm radiation energy is projected onto the aerosol or aerosol particles in the tube through the quartz transmission tube 10, and then reflected back through the mirror reflection tube 14 to irradiate repeatedly .

Among them, an integrated cooling substrate 14 is provided outside the LED array board 11, and the heat of 450nm and LEDs are all dissipated through the cooling substrate 14 through natural cooling to keep the smart atomizer in a long-term running state.

Among them, the LED array board 11 includes a plurality of LED SMD chips 111, and the LED SMD chips 111 are connected in series through series wires 112 to form a group to work in a constant current limiting mode. The positions of the positive cooling surface 113 and the negative cooling surface 114 are based on The specific parameters of the LED SMD chip 11 are separately designed positive and negative heat dissipation surfaces to ensure sufficient heat dissipation efficiency and thermoelectric separation effect. The position of the LED SMD chip 111 is the SMD 1-3W high-power 450nm, LED chip, usually It is a planar structure with no condenser mirror and an emission angle of 120 degrees.

Wherein, the center of the droplet recovery sheet 6 is provided with a square through groove 61, and a gas-liquid separation mechanism is arranged in the through groove 61. The specific structure of the gas-liquid separation mechanism is: a filter plate 1 62, a filter plate 2 63 and a filter plate 3 64 , through the inside of the groove 61, filter plate 1 62, filter plate 2 63, and filter plate 3 64 are successively arranged from bottom to top, and the gas-liquid separation apertures of filter plate 1 62, filter plate 2 63, and filter plate 3 64 are gradually reduced. The gas-liquid separation aperture of plate 3 64 is 20um, and the effect obtained is that redundant droplets or droplets with a particle size exceeding 20um are recycled back into the liquid pool.

Wherein, the left and right sides of corresponding filter plate two 63 inside the through groove 61 are all provided with movable grooves 65, the inner sides of the movable grooves 65 are set as smooth inclined surfaces, and the inclined surfaces inside the movable grooves 65 are arranged in parallel, the left and right sides of the filter plate two 63 Both sides are fixed with mounting lugs 632, and the side of the mounting lugs 632 close to the inside of the movable groove 65 fits and connects with the inclined surface inside the movable groove 65. A vertical slide bar 67 is fixed in the movable groove 65, and the mounting lugs 623 The upper end is provided with a strip-shaped adjustment hole 634, the adjustment hole 634 is sleeved on the upper end of the slide bar 67, and the bottom of the movable groove 65 is provided with a gutter 66 connected with the adjustment hole 634; The second filter plate 63 floats along the movable groove 65, and the inner side of the movable groove 65 is inclined, so that the left and right positions of the second filter plate 63 deviate during the floating process, and can better contact with the vaporized liquid droplets.

Wherein, the filter plate 2 63 is provided with a cavity 631, and the upper and lower ends of the cavity 631 are provided with several groups of filter tips 633 extending to the outside of the filter plate 63. The filter tips 633 shrink and move in the cavity 631 by the extrusion force, and the filter tips The outer sides of 633 are connected with filter plate one 62 and filter plate two 63 respectively, and are fixedly connected between filter tips 633 in the same row of cavity 631, and a spring is fixedly connected between filter tip 633 and the corresponding side in the cavity 631, filter plate two 63 initial position, the spring positioned at the upper end of the cavity is in a normal state, the spring positioned at the lower end of the cavity is in an extended state, and the filter tip 633 includes an air intake end 16 and a filter end 17, the air intake end 16 and the filter end 17 are integrally formed, and The gas end 16 is provided with an air inlet 161, and the two sides of the filter end 17 are provided with filter nets 171; while the filter plate 2 63 is rising, the filter tip 633 located at the upper end is forced to shrink into the cavity 631, but it always fits the filter Plate three 64, the filter tip 633 located at the lower end stretches out of the cavity 631 under the action of the spring, and can always be in contact with the filter plate one 62, while the filter plate two 63 rises and deviates left and right, so that the filter tip 633 can scrape and attach to the filter plate one 62 and filter plate 2 63 surface impurities or undropped liquid droplets, to achieve the cleaning effect, the filter tip 633 in the process of expansion and contraction in the cavity 631 can achieve the self-cleaning filter screen 171.

Use a pressure fan to suck the air in the environment, and fully mix the particles suspended in the air with the aqueous solution containing vitamin B2 in the water tank 1 through an air distributor 102, and then use the ultrasonic vibrator 3 to move the inside of the liquid level track tube 2 up and down Move the position with the change of the liquid level, make the aqueous solution into a liquid with a size of 1-100um, these droplets are pushed by the airflow through a Venturi tube 4, pass through the droplet recovery sheet 6, and the airflow speed at the outlet of the Venturi tube 4 is accelerated, And then the second filter plate 63 floats along the movable groove 65, and the inner side of the movable groove 65 is inclined, so that the left and right position of the second filter plate 63 is offset during the floating process, and the second filter plate 63 is positioned at the filter tip of the upper end during the rising process. 633 is stressed and shrinks toward the inside of cavity 631, but sticks to filter plate 3 64 all the time, and the filter tip 633 positioned at the lower end stretches out cavity 631 by spring action, and can also contact filter plate 1 62 all the time, while filter plate 2 63 The left and right deviation occurs in the rise, so that the filter tip 633 can scrape the impurities on the surface of the filter plate 1 62 and the filter plate 2 63 or the liquid droplets that have not dropped to achieve the cleaning effect. Self-cleaning filter screen 171, droplets pass through filter plate 1 62, filter plate 2 63, filter plate 3 64, and excess droplets fall into the droplet recovery casing 5 with funnel mouth 51 on the top through the water drop trough 66, and pass through the funnel The mouth 51 feeds into the water tank 1, and the recombined and smaller liquid droplets wrap the suspended rigid particles and push them to the quartz transmission tube 10 in the spray inactivation unit 7 to receive 450nm radiation, and After staying in the quartz transmission tube 10 for 1-10 seconds, it is re-released into the environment to suspend in the form of a stable aerosol, and the 450nm radiation energy is projected on the aerosol or aerosol particles in the tube through the quartz transmission tube 10, Then it is reflected by the mirror reflection tube 14 and irradiated repeatedly.

Claims (2)

1. The intelligent atomizer comprises a water tank (1) with a water inlet pipe (103), an air supply device (101), a fogging unit and a spray inactivation unit (7), and is characterized in that the water tank (1) with the water inlet pipe (103) is internally provided with an air distributor (102), the air supply device (101) comprises an interface for receiving an air source and an interface for outputting the air distributor (102), the fogging unit is installed at the upper end inside the water tank (1), the fogging unit comprises a liquid level track pipe (2), an ultrasonic vibrator (3), a venturi tube (4) and a liquid drop recovery sleeve (5) with a funnel opening (51) at the top, the ultrasonic vibrator (3) is arranged in the liquid level track pipe (2) and moves along with the change of the liquid level, the upper end of the ultrasonic vibrator (3) in the liquid level track pipe (2) is provided with the venturi tube (4), the upper end of the venturi tube (4) is provided with the liquid drop recovery sleeve (5) with the funnel opening (51) at the top, the top of the fogging unit is provided with the spray inactivation unit (7), the spray inactivation unit (7) is provided with a spray inactivation array plate (9), and a reflection plate (9), the upper end of the fixed seat (8) is positioned on the inner side of the reflecting tube (9) and is provided with a quartz transmission tube (10), the lower end of the quartz transmission tube (10) is communicated with the fogging unit, the outer side of the quartz transmission tube (10) is provided with an LED array plate (11), an irradiation cavity (15) is arranged between the LED array plate (11) and the quartz transmission tube (10), the center of the liquid drop recovery sheet (6) is provided with a square through groove (61), a gas-liquid separation mechanism is arranged in the through groove (61) and comprises a first filter plate (62), a second filter plate (63) and a third filter plate (64), the inside of the through groove (61) is sequentially provided with the first filter plate (62), the second filter plate (63) and the third filter plate (64) from bottom to top, the gas-liquid separation apertures of the first filter plate (62), the second filter plate (63) and the third filter plate (64) are gradually reduced, and the gas-liquid separation aperture of the third filter plate (64) is 20um; the filter comprises a first filter plate (62) and a second filter plate (63), wherein a movable groove (65) is formed in the left side and the right side of the penetrating groove (61) corresponding to the second filter plate (63), the inner side of the movable groove (65) is a smooth inclined surface, the inclined surfaces in the movable groove (65) are arranged in parallel, mounting lug plates (632) are fixed on the left side and the right side of the second filter plate (63), one side, close to the movable groove (65), of each mounting lug plate (632) is attached to the inclined surface in the movable groove (65), a cavity (631) is formed in the second filter plate (63), a plurality of groups of filter tips (633) extending to the outer side of the second filter plate (63) are arranged at the upper end and the lower end of the cavity (631), the outer side of each filter tip (633) is respectively connected with the first filter plate (62) and the second filter tip (63), the cavity (631) is fixedly connected with the filter tips (633) in a row, springs at the upper end of the cavity (631) and one side corresponding to the cavity (631), when the filter tip (63) is in the initial position, the springs at the upper end of the cavity (63), the cavity are in a normal state, the springs at the lower end of the cavity, the cavity (633) are located at the end of the filter tips (16) and at the two sides of the filter tip (17), and the filter tip (17) are integrally formed by the filter tip (17), and the filter tip (16).

2. The intelligent atomizer according to claim 1, characterized in that, the outside of the LED array plate (11) is provided with an integrally formed heat dissipation substrate (14).

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