CN113030341A

CN113030341A - System for preparing liquid phase tandem mass spectrum sample

Info

Publication number: CN113030341A
Application number: CN202110398970.1A
Authority: CN
Inventors: 陈涛; 张静; 林金飞; 王珊霞; 叶冠钦; 李德胜
Original assignee: Nanxinyi Guangzhou Manufacturing Co ltd
Current assignee: Nanxinyi Guangzhou Manufacturing Co ltd
Priority date: 2021-04-14
Filing date: 2021-04-14
Publication date: 2021-06-25
Anticipated expiration: 2041-04-14
Also published as: CN113030341B

Abstract

The invention discloses a system for preparing liquid phase tandem mass spectrometry samples. The system comprises a casing, a raw material storage module, a moving module, a processing module and a control module, and the casing forms a closed space to accommodate the raw material storage module, the moving module and the processing module; The raw material storage module is used to store the raw materials to be processed; the moving module is used to move the raw materials in the raw material module to the processing module; the processing module is used to process the raw materials received from the moving module; the processing module also includes a nitrogen blowing module, nitrogen blowing The module can blow nitrogen with positive pressure on the samples in the processing module; the control module controls the operation of the raw material storage module, the moving module and the processing module, the system for preparing liquid phase tandem mass spectrometry samples of the present invention is compatible with a variety of mass spectrometers, and realizes one-stop automation Sample preparation avoids environmental pollution and sample cross-contamination, improves the quality and efficiency of mass spectrometry samples, and meets the requirements of high-throughput and standardized operation experiments in metabolomics.

Description

System for preparing liquid phase tandem mass spectrum sample

Technical Field

The invention relates to the technical field of biomedical automated instruments, in particular to a system for preparing a liquid phase tandem mass spectrum sample.

Background

In recent years, various new inspection theories and new techniques are emerging, and the development of clinical inspection subjects is greatly promoted. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology integrates high separation performance of liquid chromatography on complex samples and high sensitivity and high specificity of mass spectrometry, is widely applied to the field of clinical examination, such as vitamin and hormone detection, neonatal genetic metabolic disease screening, therapeutic drug monitoring, drug poisoning and drug abuse analysis and other functional medical detection, and is particularly widely applied to quantitative detection of small molecular substances in biological samples. Compared with the traditional clinical test method, the LC-MS/MS technology has the advantages of high sensitivity, good specificity, high accuracy, capability of simultaneously detecting a plurality of target analytes and the like, and has good clinical application prospect; however, the method also has the problems of low automation degree, complex instrument, difficult standardization and the like.

The clinical mass spectrometry metabolic detection is carried out on complex matrix samples, such as blood samples, urine samples and the like. The amount of sample typically required to perform mass spectrometric detection analysis is small due to limitations in the detection method and the influence of metabolite signal response. The limitation of the sample size makes it very challenging to optimize an efficient extraction method in the detection process. The sample extraction usually needs protein precipitation by organic solvent, liquid-liquid extraction or solid-phase extraction, and then further separates and enriches the target metabolism, and the operation steps are complicated.

With the wide popularization of clinical mass spectrometry, the experimental requirements are increasing day by day, the requirements on analysis and detection are correspondingly and rapidly improved, and the experiment personnel are unconscious due to the complex pipetting operation and the massive sample treatment, so that the burden of the personnel is increased, and the progress of the project is slowed down. The demand of scientific laboratories for automation is increasing increasingly entering the 4.0 era of digitization and industry. The instrument and the equipment also need to obtain accurate, reliable and compliant experimental data to realize the expected analysis target through the processes of sample processing, automatic detection, information processing, analysis judgment, manipulation control and the like according to requirements under the condition of no person or less persons directly participating. Therefore, the automatic metabolite extraction instrument provides a targeted sample solution for clinical mass spectrometry users, helps the users to get rid of complicated repeated labor, liberates hands, completes the experiments with high flux and high quality, better improves reproducibility and improves experiment efficiency, and is a key node for the development of clinical mass spectrometry at present.

The amount of sample typically required to perform mass spectrometric detection analysis is small due to limitations in the detection method and the influence of metabolite signal response. The limitation of the sample size makes it very challenging to optimize an efficient extraction method in the detection process. The sample extraction usually needs protein precipitation by organic solvent, liquid-liquid extraction or solid-phase extraction, and then further separates and enriches the target metabolism, and the operation steps are complicated.

At present, clinical samples mainly comprise serum, plasma, whole blood, urine samples and the like. These sample matrices are complex and therefore appropriate sample pre-treatment methods must be selected depending on the nature of the target analyte. Common sample pretreatment methods include:

1. protein Precipitation (PPT), which is a method in which a protein precipitant is added to denature proteins in a sample, the mixture is centrifuged, and the supernatant is directly or diluted before detection.

2. Liquid-liquid extraction (LLE) methods employ water-immiscible organic solvents to extract target analytes from an aqueous phase and transfer them to an organic phase.

3. Solid-phase extraction (SPE) is a method for selectively removing interferents from a sample to obtain a target analyte suitable for mass spectrometry, wherein the target analyte is retained and eluted by using different distribution systems of different components in a sample matrix.

4. Other methods, such as ultrafiltration, dilution or derivatization, and the like. With the wide popularization of clinical mass spectrometry, the experimental requirements are increasing day by day, the requirements on analysis and detection are correspondingly and rapidly improved, and the experiment personnel are unconscious due to the complex pipetting operation and the massive sample treatment, so that the burden of the personnel is increased, and the progress of the project is slowed down.

Disclosure of Invention

The invention aims to provide a system for preparing a liquid phase tandem mass spectrometry sample, which is specially designed for preparing a clinical liquid phase tandem mass spectrometry detection sample, is compatible with various mass spectrometers, realizes one-stop automatic sample preparation, avoids environmental pollution and sample cross pollution, improves the quality and efficiency of the mass spectrometry sample, and meets the requirements of metabonomics high-throughput and standardized operation experiments.

In order to solve the above problems, the present invention provides a system for preparing a liquid phase tandem mass spectrometry sample, the system comprising a housing, a raw material storage module, a moving module, a processing module and a control module, the housing forming a closed space; the raw material storage module is used for storing raw materials to be processed; the moving module is used for moving the raw materials in the raw material module to the processing module; a processing module to process the feedstock received from the movement module; the processing module further comprises a nitrogen blowing module, and the nitrogen blowing module can seal the sample in the processing module and/or blow nitrogen at positive pressure; the control module is configured to be respectively connected with the raw material storage module, the moving module and the processing module and control the operation of the raw material storage module, the moving module and the processing module; wherein the raw material storage module, the moving module and the processing module are located in the enclosed space.

In one embodiment, the processing module comprises a nitrogen blowing module and an extraction module, the extraction module is used for performing extraction elution on raw materials or mixed samples, and the nitrogen blowing module is used for sealing and positive pressure nitrogen blowing on samples in the extraction module.

In one embodiment, the processing module further comprises a mixing module for receiving the material displaced by the moving module and a sample module for receiving the reconstituted sample displaced by the moving module.

In one embodiment, the sample module is provided with a shallow well channel that can receive the reconstituted sample.

In one embodiment, the extraction module includes a removable filter cartridge device and a collection device located below the filter cartridge device.

The filter element device is used for receiving the mixed sample moved by the moving module from the mixing module or the single raw material moved by the moving module from the raw material storage module and performing extraction elution on the mixed sample or the single raw material, and the collecting device is used for collecting the filtrate sample after the extraction elution or redissolving the filtrate into a redissolved sample.

In one embodiment, the nitrogen blow mold block comprises a sealing device and a nitrogen blow needle, the sealing device is used for sealing the filter element device, and the nitrogen blow needle is communicated with a nitrogen gas source and is used for pressing the sealed filter element device to press filtrate in the filter element device into the collecting device or perform nitrogen blow drying on the filtrate in the collecting device.

In one embodiment, the nitrogen blowing module further comprises a moving assembly and a nitrogen blowing device fixedly installed on the moving assembly, the moving assembly is connected with the nitrogen blowing device and drives the nitrogen blowing device to move, and the sealing device and the nitrogen blowing needle are installed on the nitrogen blowing device.

In one embodiment, the liquid mass spectrometry sample system is further provided with a sample feeding bottle, and the sample feeding bottle can collect filtrate in the collection module moved by the moving module.

In one embodiment, the mixing module comprises a base, a vibrator, a mixing device and a driving device, the vibrator is arranged below the mixing device, the heater is arranged below the vibrator, the base is arranged below the heater, the driving device is arranged below the base and is connected with the vibrator so as to drive the vibrator to move, and the mixing device is provided with a deep hole groove which can receive raw materials moved by the moving module from the raw material storage module.

In one embodiment, the raw material storage module comprises a sample installation device to be tested, a sample feeding bottle installation device, a gun head device and a reagent bottle installation device, wherein the sample installation device to be tested is used for placing a sample to be tested, the sample feeding bottle installation device is used for placing a sample feeding bottle, the gun head device is used for installing a disposable gun head, and the reagent bottle installation device is used for installing a reagent bottle.

In one embodiment, the sample installation device to be tested and the sample injection bottle installation device comprise a movable support and a fixed support, the movable support is detachably installed in the fixed support, a slotted hole is formed in the movable support, and the sample to be tested and the sample injection bottle are placed in the slotted hole.

In one embodiment, the liquid mass spectrometry sample system is further provided with a scanning device, bar codes are respectively pasted on the movable support and the sample to be detected, and the scanning device is used for scanning the bar codes of the movable support and the sample to be detected and positioning the input system.

In one embodiment, the moving module comprises an XY moving assembly and a moving device fixedly mounted on the XY moving assembly, and the control module controls the XY moving assembly to drive the moving device to move along an X axis and a Y axis, wherein the moving device comprises a pipette and a gripper, the pipette is provided with a mechanical arm to grip a disposable pipette head in the pipette head device, and the gripper can remove the extracted filter element device.

In one embodiment, a waste recovery module is further arranged on the liquid mass spectrometry sample system, and the waste recovery module is used for recovering the disposable gun head and the filter element device.

In one embodiment, the system for preparing the liquid phase tandem mass spectrometry sample further comprises an exhaust device, the exhaust device comprises an exhaust outlet and a filtering and adsorbing material, the exhaust outlet is arranged on the shell, and the filtering and adsorbing device is arranged in the exhaust outlet.

In one embodiment, the liquid mass spectrometry sample system further comprises a heat sink for dissipating heat generated by the system to the outside of the system.

The system for preparing the liquid phase tandem mass spectrum sample is compatible with various mass spectrometers, realizes one-stop automatic sample preparation, avoids environmental pollution and sample cross pollution, improves the quality and efficiency of the mass spectrum sample, and meets the requirements of high-throughput and standardized operation experiments of metabonomics.

Drawings

FIG. 1 is a front view of a system for preparing a LC tandem mass spectrometry sample, in accordance with one embodiment of the present invention.

FIG. 2 is a perspective view of a table, control module, storage module, processing module, and movement module of one embodiment of the invention.

FIG. 3 is a perspective view of a table, memory module and processing module of one embodiment of the present invention.

FIG. 4 is a top view of a table, storage module, and processing module of one embodiment of the invention.

Fig. 5 is a perspective view of a sample bottle mounting device and a sample mounting device to be tested according to an embodiment of the present invention.

Fig. 6 is a perspective view of an extraction module of one embodiment of the present invention.

Fig. 7 is a perspective view of an XY moving assembly of one embodiment of the present invention.

Fig. 8 is a perspective view of a mobile device according to an embodiment of the present invention.

FIG. 9 is a perspective view of a nitrogen purge module according to one embodiment of the invention.

FIG. 10 is a top view of a moving assembly of one embodiment of the present invention.

FIG. 11 is a perspective view of a nitrogen blow mold block with front and rear baffles removed in accordance with an embodiment of the present invention.

Figure 12 is a perspective view of a nitrogen blowing needle within a nitrogen blowing frame of a nitrogen blowing assembly in accordance with one embodiment of the present invention.

Figure 13 is a perspective view of the nitrogen blowing assembly with the nitrogen blowing needle moved downwardly in accordance with one embodiment of the present invention.

Detailed Description

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the objects, features and advantages of the invention can be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended to limit the scope of the present invention, but are merely intended to illustrate the spirit of the technical solution of the present invention.

In the following description, for the purposes of illustrating various disclosed embodiments, certain specific details are set forth in order to provide a thorough understanding of the various disclosed embodiments. One skilled in the relevant art will recognize, however, that the embodiments may be practiced without one or more of the specific details. In other instances, well-known devices, structures and techniques associated with this application may not be shown or described in detail to avoid unnecessarily obscuring the description of the embodiments.

Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

In the following description, for the purposes of clearly illustrating the structure and operation of the present invention, directional terms will be used, but terms such as "front", "rear", "left", "right", "outer", "inner", "outer", "inward", "upper", "lower", etc. should be construed as words of convenience and should not be construed as limiting terms.

One embodiment of the present application relates to a system for preparing a liquid phase tandem mass spectrometry sample, the system comprising a housing, a feedstock storage module, a movement module, a processing module, and a control module, the housing forming an enclosed space; the raw material storage module is used for storing raw materials to be processed; the moving module is used for moving the raw materials in the raw material module to the processing module; the processing module is used for processing the raw materials received from the moving module; the control module is configured to be respectively connected with the raw material storage module, the moving module and the processing module and control the operation of the raw material storage module, the moving module and the processing module; wherein the raw material storage module, the moving module and the processing module are positioned in a closed space formed by the shell. The system for preparing the liquid phase tandem mass spectrum sample is compatible with various mass spectrometers, realizes one-stop automatic sample preparation, avoids environmental pollution and sample cross pollution, improves the quality and efficiency of the mass spectrum sample, and meets the requirements of high-throughput and standardized operation experiments of metabonomics.

Fig. 1 is a front view of a system for preparing a tandem mass spectrometry sample according to an embodiment of the present invention, and fig. 2 to 4 are perspective views of a stage, a control module, a memory module, a processing module, and a moving module according to an embodiment of the present invention, respectively, and referring to fig. 1 to 4, a system 100 for preparing a tandem mass spectrometry sample comprises a stage 101 and a housing 102 disposed above the stage, wherein a closed space is formed between the housing 102 and a table 1011 at an upper end of the stage 101. The workbench 101 is a rectangular cabinet, and a rectangular table 1011 is formed on the upper end surface of the cabinet, and it is understood that the workbench 101 may be configured into other shapes, such as a cylinder, a cone, etc., as required. A raw material storage module 1, a processing module 2 and a moving module 3 are fixedly arranged on the table-board 1011, and the raw material storage module 1 is used for storing reagents, liquid to be detected and disposable gun heads; the processing module 2 is used for processing the raw materials received from the moving module 2; the moving module 3 is used for moving the raw materials in the raw material storage module 1 to the processing module 2; the housing 102 is disposed on the top of the table 1011 and forms an enclosed space with the table 1011, and the raw material storage module 1, the processing module 2 and the moving module 3 are mounted in the enclosed space formed by the housing 102 and the table 1011.

It is noted that in some embodiments, the table 101 of the present invention may be formed as part of the housing 102, i.e., the table is integrally formed within the housing 102. That is, the housing 102 according to the present invention forms a closed space, and the housing 102 itself may form a closed space, or the housing 102 and other components such as the table 101 may form a closed space.

The raw material storage module 1 comprises a sample feeding bottle installation device 12, a sample installation device 11 to be tested, a gun head installation device 13 and a reagent bottle installation device 14, wherein the sample feeding bottle installation device 12 is used for placing a sample feeding bottle of liquid to be tested, the sample installation device 11 to be tested is used for placing a test tube of the sample to be tested, the gun head installation device 13 is used for placing a disposable gun head, the reagent bottle installation device 14 is used for placing a reagent bottle for containing auxiliary reagent, the installation positions of the sample feeding bottle installation device 12, the sample installation device 11 to be tested, the gun head installation device 13 and the reagent bottle installation device 14 on a workbench 101 are in various modes and can be adjusted according to needs, in the embodiment shown in figures 2 and 3, the sample feeding bottle installation device 12 and the sample installation device 11 to be tested are arranged at the leftmost side (referring to the direction shown in figure 2), the gun head installation device 13 is arranged at the, the reagent bottle mounting device 14 is mounted on the right side of the lance tip mounting device 13.

The sample installation device 11 to be tested and the sample injection bottle installation device 12 comprise a movable support and a fixed support, the movable support is detachably installed in the fixed support, a slotted hole is formed in the movable support, and a sample to be tested and a sample injection bottle are placed in the slotted hole; the system 100 for preparing the LC-MS samples is further provided with a scanning device 15, wherein bar codes are respectively stuck on the movable support and the samples to be detected, and the scanning device 15 is used for scanning the bar codes of the movable support and the samples to be detected and positioning the recording system.

Fig. 5 is a perspective view of the sample installation apparatus 11 to be measured. Referring to fig. 5, the sample mounting apparatus 11 includes a plurality of rows of first movable brackets 111 and a plurality of rows of fixed brackets 112, the fixed brackets 112 are fixed on the table surface 1011 of the worktable 101, each row of the first movable brackets 111 is separately and detachably mounted on the fixed brackets 112, a plurality of rows of rails 1121 are disposed on the fixed brackets 112 so that each row of the first movable brackets 111 can be respectively and slidably mounted in each rail of the fixed brackets 112, each row of the first movable brackets 111 is a rectangular parallelepiped structure having a certain height and is sequentially provided with a plurality of test tube slots along the length direction for placing test tubes for storing liquid to be tested. An armrest 1111 is disposed at an end of each row of the first movable brackets 111 to facilitate grasping and pushing the first movable brackets 111 to slide along the rail 1121. During the use, paste the bar code in the slotted hole of first movable support 111 and on the test tube respectively, the liquid that awaits measuring is placed in the test tube after, places the test tube in the slotted hole of first movable support 111, promotes handrail 1111 and slides immigration fixed bolster 112 with the test tube together with first movable support 111, and at the in-process that first movable support 111 removed, scanning device 15 scans the bar code on first movable support 111 slotted hole and the test tube respectively and types the system, still can fix a position test tube and slotted hole one by one simultaneously.

The sample bottle installation device 12 and the sample installation device 11 to be tested share one fixed bracket 112, that is, the sample bottle installation device 12 is also installed on the fixed bracket 112, the sample bottle installation device 12 includes a plurality of second movable brackets 121, the overall shape of the second movable brackets 121 is similar to that of the first movable brackets 111, the second movable brackets 121 are also removably installed in the track of the fixed bracket 112 and are provided with slots for installing sample bottles, the depth and aperture of the slots on the second movable brackets 121 are set according to the size of the sample bottles, in general, because the size of the sample feeding bottle is different from the size of the sample test tube to be measured, the depth and the aperture of the slotted hole on the second movable support 121 are different from the depth and the aperture of the slotted hole on the first movable support 111, alternatively, the sample bottle mounting device 12 may be separately fixed on the worktable 101 instead of being arranged on the fixed bracket 112.

The gun head mounting device 13 comprises a plurality of groups of gun head supports 131 and a mounting frame 132, referring to fig. 3, the gun head supports 131 and the mounting frame 132 are correspondingly provided with 5 groups, the lower ends of the gun head supports 131 are fixed on a table surface 1011 of the workbench 101, the mounting frame 132 is detachably mounted on the gun head supports 131, each group of mounting frame 132 is provided with a plurality of gun head mounting holes, a disposable gun head is mounted in the mounting holes from top to bottom, a suction head faces the table surface 1011, the mounting frame 132 is mounted behind the gun head supports 131, and the disposable gun head is suspended in the mounting frame 131 to prevent the disposable gun head from.

The reagent bottle mounting device 14 includes a plurality of reagent bottle fixing frames 141, the reagent bottle fixing frames 141 are fixed on the table surface 1011 of the workbench 101, and a plurality of reagent bottle mounting holes are formed in the reagent bottle fixing frames 141 for mounting reagent bottles.

Optionally, the reagent bottle mounting device 14 includes a reagent bottle fixing frame and a movable frame, the movable frame is detachably mounted on the reagent bottle fixing frame, and different movable frames are provided with reagent bottle mounting holes for mounting different reagent bottles, so that different movable frames can be selected according to different reagent bottles.

Referring to fig. 3, 4 and 6, the processing module 2 includes a plurality of sets of mixing modules 21, a plurality of sets of extracting modules 22, a nitrogen blowing module 5 and a plurality of sets of sample modules 24, wherein the extracting modules 22 are used for extracting and eluting raw materials or mixed samples, the nitrogen blowing module 5 is used for sealing and positive pressure nitrogen blowing samples in the extracting modules 22, the mixing modules 21 are used for receiving raw materials moved by the moving modules 3, and the sample modules 24 are used for receiving redissolved samples moved by the moving modules 3.

In the embodiment of fig. 3 and 4, the processing module 2 comprises three sets of uniformly sized mixing modules 21, three sets of uniformly sized extraction modules 22, one set of nitrogen blow molding blocks 5 and three sets of uniformly sized sample modules 24, and the mixing modules 21, the extraction modules 22 and the sample modules 24 are all in the shape of a rectangular parallelepiped and have substantially uniform outer dimensions, the three sets of mixing modules 21 are arranged side by side in a plasma, the three sets of extraction modules 22 are arranged behind the three sets of mixing modules 21 with reference to the side by side manner of the mixing modules 21, and the three sets of sample modules 24 are also arranged between the mixing modules 21 and the extraction modules 22 with reference to the side by side manner of the mixing modules 21, and since the mixing modules 21, the extraction modules 22 and the sample modules 24 have uniform dimensions and are also uniform in the side by side manner, the mixing modules 21, the extraction modules 22 and the sample modules 24. The moving component 51 of the nitrogen blowing module 5 is arranged at the rear part of the table top of the worktable 101, and the nitrogen blowing device 50 is arranged at the rear part of the extraction module 22 and can move back and forth above the table top 1011 and can move to the upper parts of the mixing module 21, the extraction module 22 and the mixing module 21.

Alternatively, the arrangement of the three-group mixing module 21, the three-group extraction module 22 and the three-group sample module 24 is not limited to the arrangement shown in fig. 2 and 3, and may be adjusted as needed, such as placing the three-group mixing module 21 at the rear, the three-group sample module 24 at the front, and the three-group extraction module 22 between the three-group mixing module 21 and the three-group sample module 24. The three sets of mixing modules 21 are of the same specification, and each of the three sets of mixing modules includes a base 214 fixed to the table 101, a vibrator 212, a heater 213, a mixing device 211, and a driving device 215 disposed below the base 214, the vibrator 212 is disposed below the mixing device 211, the heater 213 is disposed below the vibrator 212, the base 214 is disposed below the heater 213, the driving device 215 is disposed below the base 214 and connected to the vibrator 212 to drive the vibrator 212 to move, and the driving device 215 may be, for example, a linear motor, a piezoelectric ceramic driver, or the like. The driving device 215 is located below the table 1011 and electrically connected to the control module 4. The vibrator 212 may vibrate relative to the base 214. Alternatively, the heater 213 may be provided integrally with the vibrator 212, the vibrator 212 may be a rectangular disk opened upward, the mixing device 211 may have a rectangular parallelepiped shape and may be stably installed in the vibrator 212, and is detachable from the tray of the vibrator 212, the vibrator 212 may drive the mixing device 211 to vibrate together when vibrating, the upper surface of the mixing device 211 is provided with a plurality of deep hole grooves 2111, in the embodiment shown in fig. 3, 8 rows of deep-hole slots 2111 are provided at the upper end of each set of mixing

devices

211, 12 deep-hole slots 2111 in each row, that is, each group of mixing device 211 is provided with 96 deep-hole grooves 2111 in total, when in use, the moving module 3 sucks the liquid to be tested and the reagent in each test tube in the raw material storage module 1 through the disposable gun head and then respectively moves the liquid to be tested and the reagent to different deep-hole grooves 2111, and the vibrator 212 drives the mixing device 211 to vibrate together and/or the heater 213 heats the mixing device 211 so that the liquid to be tested and the reagent are mixed more quickly.

The three groups of extraction modules 22 have the same specification, and are similar to the mixing module 21, and also include a base 214 fixed on the workbench 101, a filter element device 221 and a collecting device 222 located right below the filter element device 221 are detachably installed above the base 214, the filter element device 221 is removably installed right above the collecting device 222, the filter element device 221 is a rectangular box, the upper end surface of the box is provided with a plurality of through holes 2211, in the embodiment shown in fig. 3, each group of filter element device 221 is provided with 96 through holes 2211, the arrangement of the through holes 2211 is consistent with the arrangement of the deep hole slots 2111 of the mixing module 21, i.e. 8 rows, each row of 12 through holes 2211, the total number of 96 through holes 2211, the through holes 2211 can be provided with different extraction column fillers according to needs, the collecting device 222 and the filter element device 221 are assembled in a matching manner to collect the liquid filtered in the filter element device 221, the collecting, the upper end of the collecting device 222 is open for collecting the filtrate after the extraction and elution in the filter element device 221. When the device is used, the moving device 32 sucks the mixed liquid in the mixing module 21 by using the disposable gun head, then moves the mixed liquid to the filter element device 221, and then is filtered and extracted by the filler of the extraction column, the nitrogen blowing needle 5222 moves downwards to conduct positive pressure nitrogen blowing on the sample in the filter element device 221, so that the filtrate in the filter element device 221 is pressed into the collecting device 222 below the filter element device 221, the hand grip 324 in the moving device 32 removes the filter element device 221 to the waste recovery device 6, the nitrogen blowing needle 5222 of the nitrogen blowing module 5 penetrates through the sealing device 5223 to conduct cold shrinkage drying on the filtrate in the collecting device 222, the moving device 32 sucks the redissolved liquid in the raw material storage module by using the disposable gun head, moves the redissolved liquid to the collecting device 222 and the sample in the collecting device 222 to form redissolved liquid, and then the moving device 32 moves the redissolved liquid to the sample.

Preferably, in order to improve the positive pressure nitrogen blowing effect, the sealing device 5223 may be used to seal the filter element device 221, and then the positive pressure nitrogen blowing is performed, specifically, the sealing device 5223 is moved to a position above the filter element device 221 to seal the filter element device 221, the nitrogen blowing needle 5222 is moved to a needle hole 52231 of the sealing device 5223 to perform positive pressure nitrogen blowing on the sample in the filter element device 221, and the pressure at which the sealed nitrogen is intensively blown into the filter element device of the filter element device 221 is increased, so that the positive pressure effect is increased.

It can be understood that, according to different extraction modes, different extraction column packing materials can be installed in the filter element device 221, if the liquid to be detected needs to adopt a solid phase extraction method, a polar or non-polar chromatographic adsorbent can be installed in the filter element device as needed for extraction, such as a silica gel column.

If the liquid to be measured needs to adopt a liquid-liquid extraction method, a separating funnel can be arranged in the filter element device 221 as required to achieve separation and extraction of components in the mixture.

If the liquid to be measured needs to adopt a protein precipitation method, a high-purity protein precipitation sieve plate can be arranged in the filter element device 221 according to the needs, after the sample and the protein precipitator are mixed, the mixed solvent is pressed through the precipitation sieve plate through positive pressure blowing of nitrogen, and then filtrate is collected for instrument analysis.

Optionally, according to different liquids to be detected and extraction modes, the control module 4 may select different extraction processes, and if the liquids to be detected do not need to be mixed, the control module 4 may directly transfer the liquids to be detected to the extraction module 22 for extraction.

Alternatively, if the filtrate in the collection device 222 can be used for instrumental analysis without drying and redissolving, the control module 4 may control the moving device 32 to directly move the filtrate to the sample bottle for testing.

Alternatively, the extraction module 22 may also be provided with a vibrator 212 and a heater 213 mounted below the collection device 222 and separately electrically connected to the control module 4, the heater 213 is fixedly connected below the vibrator 212 and may vibrate with the vibrator 212 relative to the base 214, if the filtrate in the collection device 222 needs to be dried and then redissolved, the control module 4 may control the vibrator 212 to vibrate and/or control the heater 213 to heat the filtrate to accelerate the drying of the filtrate.

The three sets of sample modules 24 are respectively provided with a base 214, the base 214 is also fixedly arranged on the table surface 1011 of the workbench 101, the sample device 241 is detachably mounted on the base 214, in the embodiment shown in fig. 3, the sample device 241 is a rectangular box, each box is provided with a plurality of shallow hole grooves 2411 for receiving the reconstituted samples in the collecting device 222 moved by the moving module 3, the number of the deep hole grooves 2411 is 96, the arrangement mode of the deep hole grooves 2411 is consistent with that of the deep hole grooves 2111 of the mixing device 211, and after the reconstituted samples are placed in the shallow hole grooves 2411, the sample device 241 can be detached from the base 214 for testing.

The nitrogen blow mold block 5 includes a nitrogen blowing device 50 and a moving assembly 51, and the moving assembly 51 can move the nitrogen blowing device 50 to translate along the X-axis. The nitrogen blowing device 50 comprises a moving frame 500 and a plurality of groups of nitrogen blowing assemblies 52 arranged inside the moving frame 500, wherein the moving frame 500 can drive the nitrogen blowing assemblies 52 to translate along the X axis.

In the embodiment shown in fig. 9-11, there are 3 sets of nitrogen blowing assemblies 52, and each set of nitrogen blowing assemblies 52 is of a uniform size, it being understood that the three sets of nitrogen blowing assemblies 52 may be operated simultaneously or may be configured to be operated individually as desired.

The internal configuration of each set of nitrogen blowing assemblies 52 is uniform, each set of nitrogen blowing assemblies 52 comprises a first driving assembly 521 and a nitrogen blowing part 522, and the first driving assembly 521 is connected with the nitrogen blowing part 522 and can drive the nitrogen blowing part 522 to move up and down.

The first driving assembly 521 includes a first motor 5211, a rail plate 5212 and a first screw (not shown) disposed in the rail plate 5212, the first motor 5211 can control the first screw to rotate and drive the nitrogen blowing assembly 522 to move up and down, a first guide rail 5213 is disposed outside the rail plate 5212, and a first sensor 5214 is disposed on the rail plate 5212 or the nitrogen blowing frame 5221 for detecting the up-and-down displacement of the nitrogen blowing assembly 522

The first motor 5211 and the first sensor 5214 are electrically connected to the control module 4 via power lines and encoder lines to receive signals from the control module 4, and the control module 4 can control the operation of the first motor 5211 and the first sensor 5214 via the encoder lines.

The nitrogen blowing unit 522 includes a nitrogen blowing frame 5221, a nitrogen blowing needle 5222 and a second driving assembly 5224, the nitrogen blowing needle 5222 and the second driving assembly 5224 are installed inside the nitrogen blowing frame 5221, and the second driving assembly 5224 can drive the nitrogen blowing needle 5222 to move up and down.

Preferably, the nitrogen blowing component 522 is further provided with a sealing device 5223, and referring to fig. 12 to 13, the nitrogen blowing needle 5222, the sealing device 5223 and the second driving assembly 5224 are installed inside the nitrogen blowing frame 5221, the sealing device 5223 is installed right below the nitrogen blowing frame 5221, the second driving assembly 5224 drives the nitrogen blowing needle 5222 to move up and down, and the sealing device 5223 can enhance the positive nitrogen blowing pressure effect of the nitrogen blowing component.

The nitrogen blowing frame 5221 is a rectangular frame with an open front end, the upper part of the nitrogen blowing frame 5221 is provided with a transverse baffle 52211 for fixedly mounting the second motor 52241, the position of the baffle 52211 is determined according to the height of the second motor 52231, namely, the second motor 52241 is mounted on the upper part of the baffle 52211 and does not extend out of the upper end of the nitrogen blowing frame 5221, the two sides of the lower part of the nitrogen blowing frame 5221 are internally provided with vertical second guide rails 52212, the second guide rails 52212 are positioned on the lower part of the baffle 52211, and the side parts of the second guide rails 52212 or the needle frame 52244 are provided with a plurality of second sensors 52213 for detecting the displacement of the needle frame 52244.

The second driving assembly 5224 comprises a second motor 52241, a moving pair 52242, a second screw rod 52243, a needle holder 52244, an air inlet connector 52245 at the upper part of the needle holder 52244 and a second slider 52246 at two ends of the needle holder 52244, the lower end of the moving pair 52242 is fixedly connected with the needle holder 52244, the second screw rod 52243 is arranged inside the moving pair 52242 and connected with a second motor 52241, and the second motor 52241 drives the second screw rod 52243 to rotate and drive the moving pair 52242 to move up and down.

The lower end of the second motor 52241 is connected to a second screw 52243, it can be understood that the second screw 52243 is connected to the second motor 52241 through the baffle 52211, and the second screw 52243 rotates to drive the sliding pair 52242 to move up and down.

The lower end of the sliding pair 52242 is fixedly connected with a needle frame 52244, the needle frame 52244 is a flat plate, the nitrogen blowing needles 5222 are fixed on the needle frame 52244, the number and the arrangement mode of the nitrogen blowing needles 5222 are consistent with the through holes 2211 of the extraction module, namely 8 rows of nitrogen blowing needles in each group, 12 nitrogen blowing needles in each group, 96 nitrogen blowing needles in each group of nitrogen blowing assemblies 52, an air inlet connector 52221 communicated with the upper end of the nitrogen blowing needle 5222, an air inlet connector 52245 arranged at the upper end of the needle frame 52244 and communicated with the nitrogen blowing needle 5222, the air inlet connector 52245 communicated with an external nitrogen source, i.e., the nitrogen gas source, can communicate with the inlet port 52245 to enter the nitrogen purging needle 5222, which, in the embodiment of figure 12, in order to save materials, the air inlet connectors 52245 are provided in only one row, each air inlet connector 52245 is distributed to each nitrogen blowing needle 5222 through a longitudinal branch, that is, each air inlet 52245 can simultaneously supply air to a row of nitrogen blowing pins 5222, it is understood that the air inlet 52245 can be arranged in multiple rows, and each row can be separately connected with control nitrogen; vertical second sliders 52246 are arranged at the left end and the right end of the needle frame 52244, a second guide rail 52212 is arranged inside the nitrogen blowing frame 5221, referring to fig. 13, the second sliders 52246 at the two sides of the needle frame 52244 can slide up and down on the second guide rails 52212 at the two sides of the nitrogen blowing frame 5221, a second sensor 52213 is arranged at one side of the second guide rail, and a protruding part is arranged at one side of the second slider 52246 so as to be matched with the second sensor 52213 to detect the movement displacement of the needle frame 52244; when the positive pressure is needed, the first driving component 521 drives the nitrogen blowing frame 5221 and the sealing device 5223 to move downwards to the extraction module 22 and cover the sealing device 5223 on the filter element device 221 of the extraction module 22, the second motor 52241 controls the second screw 52243 to rotate, the second screw 52243 drives the moving pair 52242 to drive the needle frame 52244 to move so that the nitrogen blowing needle 5222 moves into the needle hole 52231 of the sealing device 5223, the nitrogen blowing needle 5222 is positioned in the needle hole 52231 and is parallel to the sealing device 5223, nitrogen flows into the nitrogen blowing needle 5222 through the air inlet connector 52245 to carry out positive pressure on liquid in the filter element device 221, and when the liquid in the collection device 222 needs to be cooled and dried, the second driving component 5224 drives the nitrogen blowing needle 5222 to pass through the needle hole 52231 of the sealing device 5223 to suspend in the collection hole in the collection device 222 and blow and dry filtrate nitrogen in the collection hole, so as to protect the sample to be tested from being polluted by external environment.

The sealing device 5223 is installed right below the nitrogen blowing frame 5221, the sealing device 5223 is a sealing plate formed by multiple layers of materials, in the embodiment shown in fig. 8, the sealing device 5223 is 3 layers, in order to better form a sealing effect, the upper layer and the lower layer are made of elastic materials, such as foamed silica gel, the middle layer is made of hard materials, the sealing device 5223 is detachably installed at the lower end of the nitrogen blowing frame 5221 and is tightly attached to the nitrogen blowing frame 5221, the sealing device 5223 can be installed at the lower end of the nitrogen blowing frame 5221 in an adhesive manner, when the nitrogen blowing frame 5221 is used, the first motor 5211 drives the nitrogen blowing frame 5221 to move downwards to the extraction module 22, and the sealing device 5223 covers and seals the filter element device 221 at the upper end of.

The sealing device 5223 is provided with a plurality of needle holes 52231, the number and arrangement of the needle holes 52231 are arranged according to the arrangement mode of the nitrogen blowing needles 5222, the diameter of the needle holes 52231 is very small and almost equal to the fineness of the nitrogen blowing needles 5222, the whole sealing device 5223 can be in a sealing state after the nitrogen blowing needles 5222 are inserted into the needle holes 52231, in the case of positive pressure, after the sealing device 38 seals the liquid in the filter element device 221, the first motor 5211 drives the nitrogen blowing needles 5222 to be inserted into the needle holes 52231, and the nitrogen in the nitrogen blowing needles 5222 exerts pressure from top to bottom to press the filtrate in the filter element device 221 to the collecting device 222; during cooling and drying, the nitrogen blowing needle 5222 moves downwards through the sealing device 5223 to the collecting device 222 for suspension and drying.

It will be appreciated that the shape and size of the lower end surfaces of the nitrogen blowing frame 5221 and the sealing device 5223 are determined by the shape and size of the upper end surface of the filter cartridge device 221, and in one embodiment, the filter cartridge device 221 is rectangular and the upper end surface is factory side, so the lower end surfaces of the nitrogen blowing frame 5221 and the sealing device 5223 are also configured to conform to the shape of the filter cartridge device 221.

The nitrogen blowing frame 5221 is provided with a first sliding block 5232, and the first motor 5211 controls the first screw to drive the first sliding block 5232 to move up and down along the first guide rail 5213 outside the guide rail plate 5212, i.e., the first motor 5211 controls the first screw to rotate and drives the first sliding block 5232 to move along the first guide rail 5213.

Preferably, in order to increase the sealing effect of the sealing device 5223, the nitrogen blowing frame 5221 is provided with the pressurizing device 523, the pressurizing device 523 comprises a pressurizing plate 5231 and a protrusion 5234 arranged at the upper end of the pressurizing plate 5231, the first slider 5232 is arranged at the back of the pressurizing plate 5231, is connected with the first screw in the guide rail plate 5212 and is matched with the first guide rail 5213 on the guide rail plate 5212 to slide up and down; a vertical third guide rail 5233 is arranged at the front end of the pressure increasing plate 5231, a third sliding block 52214 is arranged at the rear part of the nitrogen blowing frame 5221, and the third sliding block 52214 is slidably arranged in the third guide rail 5233; the length of the third guide rail 5233 is 1cm-2cm, that is, the relative movement displacement between the pressurizing plate 5231 and the nitrogen blowing frame 5221 is 1cm-2cm, that is, the third guide rail 5233 of the pressurizing plate 5231 can make the pressurizing plate 5231 slightly move up and down relative to the nitrogen blowing frame 5221 by matching with the third slide block 52214 on the nitrogen blowing frame 5221, and the short length of the third guide rail 5233 is set so as to limit the nitrogen blowing frame 5221 to greatly slide relative to the pressurizing plate 5231; a protrusion 5234 is provided on the top of the pressure increasing plate 5231, the protrusion 5234 is directly above the nitrogen blowing frame 5221, a buffer 5235, such as a spring, is provided between the protrusion 5234 and the upper end surface of the nitrogen blowing frame 5221, and the buffer 5235 is used for buffering the collision between the nitrogen blowing frame 5221 and the pressure increasing plate 5231; when the device is used, the first screw rotates to drive the first sliding block 5232 and the pressure increasing plate 5231 to move downwards until the sealing device 5223 covers the filter element device 221, the pressure increasing plate 5231 moves downwards relative to the nitrogen blowing frame 5221, and the protrusion 5234 applies downward force to the nitrogen blowing frame 5221 until the sealing device 5223 presses the filter element device 221 to realize sealing.

It is understood that the three nitrogen blowing assemblies 52 may be configured to have different specifications, for example, a first nitrogen blowing assembly having 48 nitrogen blowing needles 5222 and a second nitrogen blowing assembly having 96 nitrogen blowing needles 5222 may be configured to have different specifications according to actual requirements, and during use, the nitrogen blowing assemblies may be selected according to actual requirements.

The moving frame 500 includes a bottom 501 and two sides 502 integrally formed by extending the bottom 501, the bottom 501 is a flat plate installed inside the sliding slot 511, a moving bar 503 matching with a third screw is installed on the lower surface of the flat plate, and the third screw rotates to drive the moving bar 503 to drive the moving frame 50 to move along the X-axis.

A plurality of sensors 504 are further provided on the bottom 501 or the moving frame 500 to detect the displacement of the moving frame 500.

The parts of the two side portions 502 extending beyond the table 1011 are provided with upper and front and rear baffles to form a semi-enclosed space for accommodating the nitrogen blowing assembly 52, and in order to move the two side portions 502 on the table 1011 along the X-axis, the table 1011 is provided with two channels 1012 along the X-axis movement of the two side portions 502 for the movement of the two side portions 502, it can be understood that the three sets of mixing modules 21, the three sets of extraction modules 22 and the three sets of sample modules 24 are arranged to be located entirely between the two channels 1012, and the two channels 1012 are long enough to move the nitrogen blowing device 231 foremost to act on the foremost mixing module 21.

The moving frame 500 moves along the X-axis in cooperation with the moving module 51, and the moving module 51 includes a sliding slot 511 and a third driving module 512, and the third driving module 512 is installed inside the sliding slot 511. The sliding groove 511 is a rectangular frame with an open upper end and is integrally installed inside the workbench 101, the length of the frame is substantially equal to the length of the whole cuboid formed by the side-by-side mixing modules 21, the extraction modules 22 and the sample modules 24, the width of the frame is slightly larger than the width of the cuboid formed by the side-by-side three groups of mixing modules 21, and a plurality of sliding rails 5111 are arranged at the bottom of the sliding groove 511 to facilitate the movement of the nitrogen blowing device 50 along the X axis.

The third driving unit 512 includes a third motor 5121, a synchronous belt 5122 and a third screw, the synchronous belt is connected to 5122, the third motor 5121 drives the synchronous belt 5122 to move, in the embodiment shown in fig. 8, the third motor 5121 and the synchronous belt 5122 are disposed inside the sliding slot 511, it is understood that the third motor 5121 and the synchronous belt 5122 can also be disposed outside the front end of the sliding slot 511.

One side of the moving frame 500 is provided with a first drag chain 5112 along the X-axis direction to protect the connecting wires in the nitrogen blowing device 50. in the embodiment shown in fig. 3, the second drag chain 505 is mounted on the upper portion of the table 1011 of the worktable 101. it is understood that the second drag chain 505 may be disposed inside the worktable 101 together with the sliding groove 511.

When the nitrogen blowing device is used, the third motor 5121 drives the synchronous belt 5122, and the synchronous belt 5122 drives the third screw rod to rotate so as to drive the nitrogen blowing device 50 to move along the X axis in the sliding rail 5111. Preferably, the third screw may also be a ball screw.

Alternatively, the moving assembly 51 can be set to other modes, such as fixing frames are arranged on the left and right sides of the nitrogen blowing device 50, a moving module and a motor are arranged above the fixing frames, and the nitrogen blowing device 50 is connected with the moving module and driven by the motor to move back and forth along the moving module.

The moving module 3 includes an XY moving component 31 and a moving device 32, and the XY moving component 31 can drive the moving device 32 to move along the X axis or the Y axis.

The XY-moving unit 31 includes a U-shaped fixed frame 311 fixed to left and right sides of the table 101, a fourth driving unit 312, and a fifth driving unit 313, the fourth driving unit 312 drives the moving device to move along the Y-axis, and the fifth driving unit 313 drives the moving device 32 to move along the X-axis.

Referring to fig. 7 and 8, the fixed frame 311 includes two side portions 3111 fixed to the table 101 and a cross member 3112 integrally formed with the two side portions 3111; a first drag chain 3113 is disposed along a length direction of the cross beam at one side of the cross beam 3112, and a related connecting wire is installed inside the first drag chain 3113 to protect the connecting wire from being worn when moving along with the Y-axis moving portion 3123.

The fourth driving assembly 312 includes a fourth motor 3121, a Y-axis module 3122, and a Y-axis moving part 3123. The fourth motor 3121 is disposed on the cross beam 3112, the Y-axis moving portion 3123 is a frame mounted on the cross beam 3112, that is, the cross beam 3112 traverses through the frame of the Y-axis moving portion 3123, the Y-axis module 3122 is disposed at the upper end of the cross beam 3112 and is internally provided with a fourth screw (not shown), the Y-axis moving portion 3123 can slide along the Y-axis module 3122, the fourth screw is connected to the Y-axis moving portion 3123, and the fourth motor 3121 drives the fourth screw to rotate to drive the Y-axis moving portion 3123 to move along the Y-axis within the Y-axis module 3122.

The lower end of the Y-axis moving portion 3123 is fixedly provided with a fifth driving module 313, the fifth driving module 313 includes a fifth motor (not shown), a Y-axis connecting portion 3131, an X-module 3132, a fifth screw inside the X-axis module 3132 and an X-axis moving portion 3133, the Y-axis connecting portion 3131 is fixedly connected to the Y-axis moving portion 3123 and can drive the X-axis moving portion 3133 to move along the Y-axis, the fifth screw is arranged inside the X-axis module 3132 and is not connected to the fifth motor, the fifth motor is arranged at one end of the X-axis module 3132, the X-axis moving portion 3133 is mounted on the X-axis module 3132 and can be expected to slide along the X-axis with respect to the X-axis module 3132, the X-axis moving portion 3133 is fixedly connected to the moving device 32, and the fifth motor drives the fifth screw to rotate and drive the X-axis moving portion 3133 to slide along the X-axis in cooperation with the X-.

The moving device 32 is fixedly connected to the X-axis moving portion 3133 and moves along with the X-axis moving portion 3133, the moving device 32 includes a fixed connection plate 321 fixedly installed on the X-axis moving portion 3133, and a pipette 322 and a gripper 324 located below the fixed connection plate 321, the pipette 322 and the gripper 324 are electrically connected to the control module 4, respectively, the pipette 322 is provided with a mechanical arm 323, when in use, the control module 4 controls the mechanical arm 323 to move to the position above the gun head device 13 along the X-axis or the Y-axis to grip the disposable gun head in the gun head device 13, then controls the mechanical arm 323 to drive the disposable gun head to move to the position above the to-be-tested sample mounting device 11 to suck the liquid to be tested or move to the position above the reagent bottle mounting device 14 to suck the reagent in the reagent bottle, and then moves the liquid to-be-tested or the reagent in the disposable gun head to the mixing module 21, the used disposable gun head is removed to the waste recovery device 6, a new disposable gun head is replaced by repeated operation, after the liquid in the mixing module 21 is fully mixed, the control module 4 controls the moving module 32 to absorb the mixed liquid in the mixing module 21 and move the mixed liquid into the filter core device 221 of the extraction module 22 for extraction and elution, the control module 4 controls the nitrogen blowing module 5 to move right above the extraction module 22 and seal positive pressure nitrogen blowing to the filter core device 221, the extracted filtrate is pressed to the collection device 222, the filter core device 221 is removed to the waste recovery device 6 by the hand grip 324 in the moving device 32, and the moving device 32 continues to replace the new disposable gun head to move the filtrate in the collection device 222 to the sampling bottle for one bag test; if the filtrate needs to be dried and redissolved, the control module 4 controls the extraction module 22 to vibrate or heat for drying, the moving device 32 sucks the redissolved reagent in the raw material storage module 1 to the collection device 222 for redissolving, and then the redissolved liquid is moved to the sample module 24 to complete sample preparation.

Preferably, the first screw, the second screw 52243, the third screw, the fourth screw and the fifth screw are all ball screws.

It will be appreciated that the movement means 32 can be adjusted according to the extraction mode, and that the disposable tips, after having extracted liquid, are removed to the waste recovery device 6 and replaced by new disposable tips.

The control module 4 is arranged outside the workbench 101, is respectively connected with the raw material storage module 1, the processing module 2 and the moving module 3, and controls the operation of the raw material storage module 1, the processing module 2 and the moving module 3, and the control module 4 can design different processes according to different extraction mode codes and correspondingly adjust the operation of the moving module 3 and the processing module 2.

The waste recovery device 6 is arranged inside the workbench 101, and a feeding port 61 is arranged on the table surface 1011 of the workbench 101 so as to collect the used disposable gun head or filter core device 221.

An automatic door is arranged in front of the shell 102 to facilitate storage of raw materials or extraction of samples to be tested, and a perspective door can be arranged on the automatic door and/or two sides of the shell 102 to facilitate operation of the observation system.

An ultraviolet sterilizing lamp 9 is arranged at the upper position inside the shell 102 and is used for performing ultraviolet sterilization and disinfection on the sealed cavity.

Exhaust device 7 is established at shell 102 top to exhaust device 7 includes air exit 71, and the air exit sets up on shell 102, installs air filter or adsorbing material in the air exit and keeps the inside pollution-free state that is in of system with filtered air impurity, and exhaust device 7 links to each other with outside laboratory exhaust duct, and entire system forms the negative pressure enclosure space.

The inside of the working table 101 is also provided with a heat sink 8 for discharging heat inside the system outside the system.

While the preferred embodiments of the present invention have been illustrated and described in detail, it should be understood that various changes and modifications of the invention can be effected therein by those skilled in the art after reading the above teachings of the invention. Such equivalents are intended to fall within the scope of the claims appended hereto.

Claims

1. A system for preparing a liquid phase tandem mass spectrometry sample, comprising:

a housing forming an enclosed space;

the raw material storage module is used for storing raw materials to be processed;

a moving module for moving the feedstock within the feedstock storage module to a processing module;

a processing module for processing the feedstock received from the movement module; and

a control module configured to be connected with the raw material storage module, the moving module and the processing module respectively and control the raw material storage module, the moving module and the processing module to operate;

wherein the feedstock storage module, the movement module, and the processing module are located within the enclosed space.

2. The system for preparing LC MS samples of claim 1, wherein said processing module comprises a nitrogen blowing module and an extraction module, said extraction module is used for performing extraction elution on raw material or mixed samples, and said nitrogen blowing module is used for sealing and positive pressure nitrogen blowing on samples in said extraction module.

3. The system for preparing liquid phase tandem mass spectrometry samples of claim 2, wherein the processing module further comprises a mixing module for receiving the feedstock displaced by the moving module and a sample module for receiving the reconstituted sample displaced by the moving module.

4. The system for preparing LC MS samples of claim 3, wherein said extraction module comprises a removable filter cartridge device and a collection device located below said filter cartridge device,

the filter element device is used for receiving the mixed sample moved by the moving module from the mixing module or the single raw material moved by the moving module from the raw material storage module and carrying out extraction elution on the mixed sample or the single raw material,

the collecting device is used for collecting the filtrate sample after extraction and elution or redissolving the filtrate into a redissolved sample.

5. The system for preparing liquid phase tandem mass spectrometry samples according to claim 4, wherein the nitrogen blow mold block comprises a sealing device and a nitrogen blow needle, the sealing device is used for sealing the filter element device, the nitrogen blow needle is communicated with a nitrogen gas source and presses the sealed filter element device to press filtrate in the filter element device into the collecting device or perform nitrogen blow drying on the filtrate in the collecting device.

6. The system for preparing the LC MS samples as claimed in claim 5, wherein the nitrogen blowing module further comprises a moving assembly and a nitrogen blowing device fixedly mounted on the moving assembly, the moving assembly is connected with the nitrogen blowing device and drives the nitrogen blowing device to move, and the sealing device and the nitrogen blowing needle are mounted on the nitrogen blowing device.

7. The system for preparing LC MS samples of claim 4, wherein said mixing module comprises a base, a heater, a vibrator, a mixing device and a driving device,

the vibrator is arranged below the mixing device, the heater is arranged below the vibrator, the base is arranged below the heater, the driving device is arranged below the base and connected with the vibrator to drive the vibrator to move, and the vibrator is arranged on the base and driven by the driving device to move

The mixing device is provided with a deep hole groove which can receive the mixed sample moved by the moving module from the raw material storage module.

8. The system for preparing LC MS samples as claimed in claim 4, wherein the raw material storage module comprises a sample installation device to be tested, a sample bottle installation device, a gun head device and a reagent bottle installation device, the sample installation device to be tested is used for placing a sample to be tested, the sample bottle installation device is used for placing a sample bottle, the gun head device is used for installing a disposable gun head, and the reagent bottle installation device is used for installing a reagent bottle.

In one embodiment, the sample device to be tested and the sample bottle device comprise a movable support and a fixed support, the movable support is detachably mounted in the fixed support, a slot is formed in the movable support, and the sample to be tested and the sample bottle are placed in the slot.

9. The system for preparing LC MS samples according to claim 8, wherein the moving module comprises an XY moving assembly and a moving device fixedly mounted on the XY moving assembly, the control module controls the XY moving assembly to drive the moving device to move along X-axis and Y-axis, wherein the moving device comprises a liquid transferring device and a gripper, the liquid transferring device is provided with a mechanical arm to grip the disposable tip, and the gripper can remove the extracted filter element device.

10. The system for preparing the LC MS samples as claimed in claim 1, further comprising an air exhaust device, wherein the air exhaust device comprises an air exhaust opening and a filtering and adsorbing material, the air exhaust opening is disposed on the housing, and the filtering and adsorbing material is mounted in the air exhaust opening.