CN111518670A - Preparation method of biochip based on silicon carbide substrate - Google Patents
Preparation method of biochip based on silicon carbide substrate Download PDFInfo
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- CN111518670A CN111518670A CN202010386497.0A CN202010386497A CN111518670A CN 111518670 A CN111518670 A CN 111518670A CN 202010386497 A CN202010386497 A CN 202010386497A CN 111518670 A CN111518670 A CN 111518670A
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Abstract
A method for preparing a biochip based on a silicon carbide substrate belongs to the field of biochips. The invention relates to a biochip based on a silicon carbide substrate, which comprises a graphene coating film, a silicon carbide substrate base and a biochip unit, wherein the graphene coating film is attached to the silicon carbide substrate base, the biochip unit is integrated on the graphene coating film, and the preparation method comprises the following steps: a, attaching a graphene coating film on a silicon carbide substrate base; b, machining and manufacturing a micro-channel on the silicon carbide substrate processed in the step a by adopting cutting, grinding and polishing processes; c, fixedly mounting a gene probe and a chemical modification unit on the micro-flow channel processed in the step b; and d, processing and manufacturing the biochip unit on the graphene coated film processed in the step c by adopting cutting, grinding and polishing processes.
Description
Technical Field
The invention relates to a preparation method of a biochip based on a silicon carbide substrate, and belongs to the field of biochips.
Background
The mechanisms of carcinogenesis are very complex, and the molecular mechanisms and histopathological pathways leading to carcinogenesis vary from cancer to cancer and even from patient to patient with the same cancer. In the process of cancer treatment, only when the patients are treated differently and are individually treated according to different people, the medicines suitable for the patients can be selected according to different types of patients, so that the cancer patients need to carry out multiple detections on different periods and different parts of the patients in the whole process from diagnosis to treatment, and the individual treatment can be carried out according to the characteristics.
At present, cancer detection must rely on large-scale or detection instrument that can't easily carry, and the sample collection back must be sent to professional medical detection institution and detect, therefore, the time of cancer test result is long, and the expense is high, and a lot of cancer patients can not be found at the early stage of cancer, delays best treatment time. The invention researches a biochip based on a silicon carbide substrate, and the silicon carbide substrate has the advantages of high conductivity and sensitivity, strong stability, high accuracy, small volume and low power consumption, and is a necessary way for realizing information medical treatment at present.
Therefore, it is necessary to provide a method for preparing a biochip based on a silicon carbide substrate to solve the above technical problems.
Disclosure of Invention
The present invention has been developed in order to solve the problems of long detection time, high cost and delayed treatment opportunity in the manner of heart cancer detection, and a brief summary of the invention is provided below to provide a basic understanding of some aspects of the invention. It should be understood that this summary is not an exhaustive overview of the invention. It is not intended to determine the key or critical elements of the present invention, nor is it intended to limit the scope of the present invention.
The technical scheme of the invention is as follows:
the utility model provides a biochip based on carborundum substrate, includes graphite alkene tectorial membrane, carborundum substrate base and biochip unit, and graphite alkene tectorial membrane is attached to carborundum substrate base, and biochip unit integration is on graphite alkene tectorial membrane.
Preferably: the biochip unit comprises a biochip system, a micropump, a plasma system, a color recognition system, a temperature control system, a microwave cleaning system and a power supply system, wherein the biochip system, the micropump, the plasma system, the color recognition system, the temperature control system and the microwave cleaning system are integrated on the graphene coating film.
Preferably: also comprises a gene probe which is arranged on the biochip system.
Preferably: a targeting probe is arranged in the plasma system.
A preparation method of a biochip based on a silicon carbide substrate comprises the following preparation steps:
a, attaching a graphene coating film on a silicon carbide substrate base;
b, machining and manufacturing a micro-channel on the silicon carbide substrate processed in the step a by adopting cutting, grinding and polishing processes;
c, fixedly mounting a gene probe and a chemical modification unit on the micro-flow channel processed in the step b;
and d, processing and manufacturing the biochip unit on the graphene coated film processed in the step c by adopting cutting, grinding and polishing processes.
Preferably: and d, integrating the biochip unit with a graphene coating film by using the biochip system, the micropump, the plasma system, the color recognition system, the temperature control system, the microwave cleaning system and the power supply system.
The invention has the following beneficial effects:
1. the biochip based on the silicon carbide substrate has the advantages of high sensitivity, high accuracy and good stability;
2. the biochip based on the silicon carbide substrate has the advantages of simple structure, small volume, convenience in carrying and transportation and storage, lower cost, medical resource saving and suitability for popularization and use;
3. the biochip based on the silicon carbide substrate saves the time that a patient needs to queue for detection and a sample is sent to a professional medical detection mechanism for detection in the cancer detection process, and increases the treatment time of a doctor;
4. the preparation method of the biochip based on the silicon carbide substrate is ingenious in preparation steps and simple in preparation process, and improves the production efficiency;
drawings
FIG. 1 is a schematic structural view of a biochip based on a silicon carbide substrate;
FIG. 2 is a schematic flow chart of a method for preparing a biochip based on a silicon carbide substrate;
FIG. 3 is a schematic view of an inspection process of a biochip based on a silicon carbide substrate;
in the figure, 1-graphene coating, 2-silicon carbide substrate, 3-biochip unit, 3-1-biochip system, 3-2-micropump, 3-3-plasma system, 3-4-color recognition system, 3-5-temperature control system and 3-6-microwave cleaning system.
Detailed Description
In order that the objects, aspects and advantages of the invention will become more apparent, the invention will be described by way of example only, and in connection with the accompanying drawings. It is to be understood that such description is merely illustrative and not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
The connection mentioned in the invention is divided into fixed connection and detachable connection, the fixed connection is non-detachable connection and includes but is not limited to folding edge connection, rivet connection, bonding connection, welding connection and other conventional fixed connection modes, the detachable connection includes but is not limited to threaded connection, snap connection, pin connection, hinge connection and other conventional detachment modes, when the specific connection mode is not clearly limited, at least one connection mode can be found in the existing connection modes by default to realize the function, and the skilled person can select according to the needs. For example: the fixed connection selects welding connection, and the detachable connection selects hinge connection.
The first embodiment is as follows: the embodiment is described with reference to fig. 1-2, and the biochip based on a silicon carbide substrate of the embodiment includes a graphene coating 1, a silicon carbide substrate base 2 and a biochip unit 3, the graphene coating 1 is attached to the silicon carbide substrate base 2, the biochip unit 3 is integrated on the graphene coating 1, the graphene coating 1 has strong conductivity, and when a gene probe in the biochip unit 3 on graphene reacts with a sample to be tested, weak current is generated.
The second embodiment is as follows: referring to FIGS. 1 to 3, the present embodiment is described, based on the first embodiment, a biochip based on a silicon carbide substrate of the present embodiment, the biochip unit 3 includes a biochip system 3-1, a micropump 3-2, a plasma system 3-3, a color recognition system 3-4, a temperature control system 3-5, a microwave cleaning system 3-6 and a power supply system, the biochip system 3-1, the micropump 3-2, the plasma system 3-3, the color recognition system 3-4, the temperature control system 3-5 and the microwave cleaning system 3-6 are integrated on a graphene coating film 1,
a biochip based on silicon carbide substrate is used in combination with a detection device, a color recognition system 3-4 is used for analyzing data of the color recognition system by reacting color to a receiver of the detection device, the receiver receives signals and reacts to a terminal of the detection device, and a control system of the detection device can analyze the data to obtain a detection result.
The third concrete implementation mode: the embodiment is described with reference to fig. 1-3, and the silicon carbide substrate-based biochip of the embodiment further includes a gene probe disposed on the biochip system 3-1, the gene probe being used to react with a sample to be tested.
The fourth concrete implementation mode: the embodiment is described with reference to fig. 1 to 3, and a biochip based on a silicon carbide substrate according to the embodiment is provided with a target probe in a plasma system 3-3, the target probe reacts with a sample to be detected to generate a change of electrons, then the current is transmitted to a power supply system through the action of a micro pump 3-2 to generate a change of the current and the intensity, the change of the current is transmitted to a color recognition system 3-4, the concentration of the sample to be detected is determined according to the change of the color, quantitative detection is realized, and a microwave cleaning system is used for cleaning the plasma system.
The fifth concrete implementation mode: the present embodiment will be described with reference to fig. 1 to 3, and the method for manufacturing a biochip based on a silicon carbide substrate according to the present embodiment includes the following steps:
a, attaching a graphene coating film 1 on a silicon carbide substrate base 2;
b, machining and manufacturing a micro-channel on the silicon carbide substrate 2 machined in the step a by adopting cutting, grinding and polishing processes;
c, fixedly mounting a gene probe and a chemical modification unit on the micro-flow channel processed in the step b;
and d, processing and manufacturing the biochip unit 3 on the graphene coated film 1 processed in the step c by adopting cutting, grinding and polishing processes.
The sixth specific implementation mode: in the method for preparing a biochip based on a silicon carbide substrate according to the present embodiment, in step d, the biochip unit 3 includes a biochip system 3-1, a micropump 3-2, a plasma system 3-3, a color recognition system 3-4, a temperature control system 3-5, a microwave cleaning system 3-6, and a power supply system, and the biochip system 3-1, the micropump 3-2, the plasma system 3-3, the color recognition system 3-4, the temperature control system 3-5, and the microwave cleaning system 3-6 are integrated on the graphene coating film 1.
It should be noted that, in the above embodiments, as long as the technical solutions can be aligned and combined without contradiction, those skilled in the art can exhaust all possibilities according to the mathematical knowledge of the alignment and combination, and therefore, the present invention does not describe the technical solutions after alignment and combination one by one, but it should be understood that the technical solutions after alignment and combination have been disclosed by the present invention.
This embodiment is only illustrative of the patent and does not limit the scope of protection thereof, and those skilled in the art can make modifications to its part without departing from the spirit of the patent.
Claims (5)
1. A biochip based on a silicon carbide substrate comprises a graphene coating film (1), a silicon carbide substrate base (2) and a biochip unit (3), wherein the graphene coating film (1) is attached to the silicon carbide substrate base (2), and the biochip unit (3) is integrated on the graphene coating film (1).
2. A silicon carbide substrate-based biochip according to claim 1, wherein: the biochip unit (3) comprises a biochip system (3-1), a micropump (3-2), a plasma system (3-3), a color recognition system (3-4), a temperature control system (3-5), a microwave cleaning system (3-6) and a power supply system, wherein the biochip system (3-1), the micropump (3-2), the plasma system (3-3), the color recognition system (3-4), the temperature control system (3-5) and the microwave cleaning system (3-6) are integrated on the graphene coating film (1).
3. A silicon carbide substrate-based biochip according to claim 2, further comprising a gene probe provided on the biochip system (3-1).
4. A preparation method of a biochip based on a silicon carbide substrate comprises the following preparation steps:
a, attaching a graphene coating (1) on a silicon carbide substrate (2);
b, machining and manufacturing a micro-channel on the graphene coating (1) with the silicon carbide substrate base processed in the step a by adopting cutting, grinding and polishing processes;
c, fixedly mounting a gene probe and a chemical modification unit on the micro-flow channel processed in the step b;
and d, processing and manufacturing the biochip unit (3) on the graphene coating film (1) processed in the step c by adopting cutting, grinding and polishing processes.
5. The method for preparing a biochip based on a silicon carbide substrate according to claim 4, wherein: in the step d, the biological chip unit (3) comprises a biological chip system (3-1), a micropump (3-2), a plasma system (3-3), a color recognition system (3-4), a temperature control system (3-5), a microwave cleaning system (3-6) and a power supply system, wherein the biological chip system (3-1), the micropump (3-2), the plasma system (3-3), the color recognition system (3-4), the temperature control system (3-5) and the microwave cleaning system (3-6) are integrated on the graphene coating film (1).
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Citations (6)
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| JP2005013905A (en) * | 2003-06-27 | 2005-01-20 | Sumitomo Electric Ind Ltd | Microchip for chemical reaction and manufacturing method thereof |
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| CN110174197A (en) * | 2019-05-28 | 2019-08-27 | 北京旭碳新材料科技有限公司 | Graphene-based piezoresistive pressure sensor and preparation method thereof |
| CN110556283A (en) * | 2018-05-30 | 2019-12-10 | 山东大学 | A method for preparing graphene and graphene devices by pretreatment of SiC substrate epitaxy |
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2020
- 2020-05-09 CN CN202010386497.0A patent/CN111518670A/en active Pending
Patent Citations (6)
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| JP2005013905A (en) * | 2003-06-27 | 2005-01-20 | Sumitomo Electric Ind Ltd | Microchip for chemical reaction and manufacturing method thereof |
| CN102245289A (en) * | 2008-10-10 | 2011-11-16 | 万罗赛斯公司 | Process and apparatus employing microchannel process technology |
| JP2011230959A (en) * | 2010-04-27 | 2011-11-17 | Kazuto Yamauchi | METHOD FOR GRAPHENE FILM DEPOSITION ON SiC SUBSTRATE AND SiC SUBSTRATE WITH GRAPHENE |
| CN110556283A (en) * | 2018-05-30 | 2019-12-10 | 山东大学 | A method for preparing graphene and graphene devices by pretreatment of SiC substrate epitaxy |
| CN110174197A (en) * | 2019-05-28 | 2019-08-27 | 北京旭碳新材料科技有限公司 | Graphene-based piezoresistive pressure sensor and preparation method thereof |
| CN110618257A (en) * | 2019-08-30 | 2019-12-27 | 北京祥为信息技术有限公司 | Biological chip and preparation method thereof |
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