CN110129186B

CN110129186B - Cell culture aseptic sampling device

Info

Publication number: CN110129186B
Application number: CN201910457413.5A
Authority: CN
Inventors: 路军秀; 朱茉莉; 郭超; 祝田田; 薛金涛; 李鹏; 房志鑫; 李伟
Original assignee: Zhejiang Meisen Cell Technology Co ltd
Current assignee: Zhejiang Meisen Cell Technology Co ltd
Priority date: 2019-05-29
Filing date: 2019-05-29
Publication date: 2022-08-16
Anticipated expiration: 2039-05-29
Also published as: CN110129186A

Abstract

The invention relates to a cell culture sterile sampling device, which effectively solves the problems of complicated operation and high cost of maintaining a sterile environment of the conventional sterile sampling mode; the technical scheme of its solution includes the incubator, from left to right the interval equipartition in proper order has multiunit sampling unit in the incubator, multiunit sampling unit highly from left to right scales up in proper order in the incubator, every group sampling unit includes a pipe, there is the passageway of multiunit on the pipe, every group passageway includes a plurality of first logical grooves, there is fixed pipe the pipe outside, be equipped with a plurality of and first logical groove one-to-one's second logical groove on the fixed pipe, there is the elbow at the front end of every pipe, the elbow inner is equipped with the syringe needle, be equipped with the rubber dish that can be with its shutoff in the pipe, the rear end of pipe is fixed with a carousel, be equipped with a first through-hole on the carousel excircle face, the sleeve is installed in the outside of carousel, the circumference equipartition has four second through-holes on the sleeve, four second through-hole clockwise are connected with high pressurized air source, the disinfection water source, clear water source and cell nutrient solution source.

Description

Cell culture aseptic sampling device

Technical Field

The invention relates to the field of cell sampling, in particular to a cell culture sterile sampling device.

Background

Cell culture is an important method for researching cell biology, some living cells need to be taken out frequently for research and experiment in the process of cell culture, cells need to be cultured in a constant-temperature sterile environment after being isolated, most of the traditional cell culture is to place an incubator in a sterile chamber and culture in the incubator, when an experiment sample is needed, a part of cell culture solution is sucked through a suction pipe for research after a series of sterilization operations, the sampling mode seems simple but is very complicated in actual operation, frequent sterile sterilization wastes scientific research time, each sterile sterilization is carried out manually, the mode has great risk of causing bacteria to be mixed in the incubator, once the bacteria are mixed in the incubator, the cells lose effect, and the maintenance of the sterile environment also needs to consume a great deal of time and capital, for some scientific research units with insufficient scientific research funding, a new sterile sampling mode is urgently needed to solve the problem at the present stage.

Disclosure of Invention

Aiming at the situation, in order to overcome the defects of the prior art, the invention provides the cell culture sterile sampling device, which effectively solves the problems of complicated operation and high maintenance cost of a sterile environment of the conventional sterile sampling mode.

The technical scheme for solving the problems comprises a closed incubator, a plurality of groups of sampling units are uniformly distributed at intervals from left to right in the incubator in sequence, the heights of the plurality of groups of sampling units in the incubator are sequentially increased from left to right, each group of sampling units comprises a circular tube, two ends of the circular tube penetrate through and extend out of the front side wall and the rear side wall of the incubator respectively, the circular tube can rotate and can move back and forth, a plurality of groups of channels are arranged at intervals side by side along the axis of the circular tube in the incubator, each group of channels comprises a plurality of first through grooves which are uniformly distributed on the circular tube in a circumferential manner, a fixed tube is coaxially fixed on the outer side of the part of the circular tube in the incubator and can move along the wall in the fixed tube, a plurality of second through grooves which correspond to the first through grooves one by one are arranged on the fixed tube, an elbow, one end of which is inserted into the circular tube and can slide along the wall in the fixed tube, the front end of the elbow faces downwards, the elbow inserts the one end of pipe and is equipped with the syringe needle, be equipped with the rubber disc that can be with its shutoff in the pipe, the pipe moves forward and can make the syringe needle insert the rubber disc and make pipe and return bend intercommunication, the rear end of pipe is fixed with a carousel, be equipped with a first through-hole with the pipe intercommunication on the outer disc of carousel, the outside coaxial arrangement of carousel has a sleeve, the sleeve inner fringe face and carousel outer fringe face adherence sliding connection, telescopic front side circumference equipartition has four second through-holes, begin from the second through-hole that is located the upper right end in four second through-holes, clockwise has connected gradually high pressurized air source, the disinfection water source, clear water source and cell nutrient solution source.

The invention has novel concept, ingenious structure and strong practicability, and effectively solves the problems of complicated operation and high cost of maintaining the sterile environment of the existing sterile sampling mode.

Drawings

FIG. 1 is a front view of the present invention.

Fig. 2 is a rear view of the present invention.

Fig. 3 is a left sectional view of the sampling unit when the first through groove and the second through groove are not communicated.

Fig. 4 is a left sectional view of the sampling unit of the present invention when the first through groove is communicated with the second through groove.

FIG. 5 is a front sectional view of the incubator and its attached structures according to the present invention.

Fig. 6 is a left side partial cross-sectional view of the present invention.

Fig. 7 is a cross-sectional end view of a first through slot in a round tube of the present invention in communication with a second through slot in a stationary tube.

Fig. 8 is a cross-sectional view of the end surface of the turntable mated with the sleeve when the first through slot communicates with the second through slot in accordance with the present invention.

Detailed Description

The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.

As can be seen from the drawings 1 to 8, the invention comprises a closed incubator 1, a plurality of groups of sampling units are uniformly distributed in the incubator 1 at intervals from left to right, the heights of the plurality of groups of sampling units in the incubator 1 are sequentially increased from left to right, each group of sampling units comprises a circular tube 2, two ends of which respectively penetrate through and extend out of the front side wall and the rear side wall of the incubator 1, the circular tubes 2 can rotate and move back and forth, a plurality of groups of channels are arranged in parallel and at intervals along the axis of the circular tube 2 in the incubator 1, each group of channels comprises a plurality of first through grooves 3 which are uniformly distributed on the circular tubes 2 in a circumferential manner, a fixed tube 4 is coaxially fixed on the outer side of the part of the circular tube 2 in the incubator 1, the circular tubes 2 can move in an adherence manner in the fixed tube 4, a plurality of second through grooves 5 which correspond to the first through grooves 3 in a one-to-one manner are arranged on the fixed tube 4, an elbow 6, one end of which is inserted into the circular tube 2 and can slide in the adherence with the circular tube 2 is fixed at the front end of each circular tube 2, the other end of elbow 6 is down, the one end that elbow 6 inserted pipe 2 is equipped with syringe needle 7, be equipped with the rubber disk 8 that can be with its shutoff in the pipe 2, pipe 2 moves forward can make syringe needle 7 insert rubber disk 8 and make pipe 2 and return bend intercommunication, the rear end of pipe 2 is fixed with a carousel 9, be equipped with a first through-hole 10 with pipe 2 intercommunication on the carousel 9 excircle face, a sleeve 11 of outside coaxial arrangement of carousel 9, sleeve 11 inner fringe face and carousel 9 outer fringe face adherence sliding connection, four second through-holes 12 are equipped with to the preceding side circumference equipartition of sleeve 11, begin from the second through-hole 12 that is located the upper right end in four second through-holes 12, clockwise has connected gradually high pressurized air source 13, disinfection water source 14, clear water source 15 and cell nutrient solution source 16.

In order to facilitate cell stirring in the incubator 1, a stirring shaft 17 is arranged between every two adjacent sampling units, one end of the stirring shaft 17 is arranged in the incubator 1, a plurality of stirring rods 18 are uniformly distributed on the circumference of the stirring shaft 17, the other end of the stirring shaft is arranged outside the incubator 1, belt wheels 19 are coaxially fixed on the other end of the stirring shaft, the belt wheels 19 are connected through belts, a first bevel gear 20 is coaxially fixed above the belt wheel 19 at the leftmost end, and a second bevel gear 21 driven by a motor is meshed above the first bevel gear 20.

In order to facilitate the forward and backward movement and rotation of the circular tubes 2, an end cam 22 is sleeved and fixed at the front end of each circular tube 2, an upright column 23 capable of being matched with the end cam 22 is further arranged above each circular tube 2, and when the pushing distance of the end cam 22 corresponds to the upright column 23, the end cam 22 is rotated to enable the circular tubes 2 to move forward while rotating.

In order to reset the circular tube 2, a tension spring 24 is arranged on the rear side of the rotary table 9, one end of the tension spring 24 is fixed with the rotary table 9, and the other end of the tension spring is fixed on the rear end face of the sleeve 11.

In order to facilitate the installation of the bent pipes, an L-shaped rod 25 is fixed at the upper end of each bent pipe, the vertical rod end of each L-shaped rod 25 is fixed with the bent pipe, and the transverse rod end is fixed on the incubator 1.

In order to realize that the disinfection water source 14, the clean water source 15 and the cell nutrient solution source 16 can conveniently flow into the round pipe 2, pressure bottles capable of providing pressure for the disinfection water source 14, the clean water source 15 and the cell nutrient solution source 16 are connected to the bearing box bodies of the disinfection water source 14, the clean water source 15 and the cell nutrient solution source 16.

The specific working process of the invention is as follows: when the cell culture incubator is used, cell nutrient solution is filled into the incubator 1 through the liquid inlet on the incubator 1 and is connected with an oxygen source, so that all temperature conditions and oxygen conditions meeting the cell growth are kept in the incubator 1, the liquid inlet is sealed after the cells are placed after the filling is finished, at the moment, the motor is started to enable the motor to drive the belt pulleys 19 through the first bevel gear 20 and the second bevel gear 21, and the belt pulleys 19 are connected through the belt, so that the stirring shafts 17 drive the stirring rods 18 to stir the cell nutrient solution, and the cells can be fully dispersed in the incubator 1.

Make the nearly end of repose of the face cam 22 among a plurality of sampling units under the initial condition correspond with stand 23, at this moment under the effect of extension spring 24 the pipe 2 moves to a plurality of first logical groove 3 on it and a plurality of first logical groove 3 on the fixed pipe 4 one-to-one and be linked together, be linked together with incubator 1 inner cell liquid in the pipe 2 this moment, because pipe 2 moves backward this moment, because syringe needle 7 on the return bend at this moment does not contact rubber disc 8, consequently return bend and pipe 2 do not communicate.

When sampling is needed, the end face cam 22 on the sampling units with different heights is selected to rotate clockwise, the end face cam 22 drives the circular tube 2 to rotate when rotating, the pushing range of the end face cam 22 is simultaneously contacted with the upright column 23, at the same time, the circular tube 2 moves forwards while rotating, in the process, the circular tube 2 drives the rubber disc 8 inside the circular tube 2 to approach the needle head 7 and the needle head 7 gradually penetrates into the rubber disc 8 to realize the communication between the circular tube 2 and the bent tube, at the same time, due to the rotation and the forward movement of the circular tube 2, the first through groove 3 on the circular tube 2 is not communicated with the second through groove 5 on the fixed tube 4, at the same time, the first through groove 3 on the circular tube 2 is blocked by the tube wall of the fixed tube 4, at the moment, the circular tube 2 is isolated from the liquid in the incubator 1, then the needle head 7 penetrates through the rubber disc 8 to realize the communication between the circular tube 2 and the outside, then the end face cam 22 is rotated, the distal stopping end of the end face cam 22 is corresponding to and contacted with the upright column 23, at this time, the end cam 22 is rotated to enable the first through hole 10 on the rotary disk 9 to be communicated with the second through hole 12 on the sleeve 11 connected with the high-pressure air source 13, and the cell culture solution in the circular tube 2 is extruded out under the action of the high-pressure air source 13 and is received through the test tube.

After sampling is finished, the end face cam 22 is continuously rotated, the far rest end of the end face cam 22 still corresponds to the upright column 23, the end face cam 22 drives the circular tube 2 to rotate, the rotary disc 9 is communicated with the second through hole 12 communicated with the disinfection water source 14, the disinfection water source 14 rapidly flows through the circular tube 2 after being pressurized by the pressure bottle and disinfects the inner wall of the circular tube 2 for one time, then the end face cam 22 is rotated, the far rest end of the end face cam 22 still corresponds to the upright column 23, the end face cam 22 drives the rotary disc 9 through the circular tube 2 to enable the first through hole 10 on the end face cam 22 to be communicated with the clear water source 15, clear water enters the circular tube 2 under the action of the pressure bottle to flush and clean the poison, the end face cam 22 is rotated again, the far rest end of the end face cam 22 still corresponds to the upright column 23, the first through hole 10 on the rotary disc 9 is communicated with the second through hole 12 of the cell nutrient solution source 16, and the circular tube 2 is filled with cell nutrient solution, at this moment, the return stroke of the end cam 22 starts from the correspondence of the upright column 23 and realizes the backward reset of the circular tube 2 under the reset action of the tension spring 24, at this moment, the rubber disc 8 gradually moves backward in the reset process of the circular tube 2 so that the finger needle 7 gradually separates from the rubber plate, under the elastic property of the rubber disc 8, the rubber disc 8 is automatically sealed, at this moment, the end cam 22 gradually rotates to the initial state, namely, the near-rest end of the end cam 22 corresponds to the upright column 23, in this process, the first through groove 3 on the circular tube 2 gradually corresponds to the second through groove 5 on the fixed tube 4, and under the stirring action of the stirring rod 18, the cells are dispersed into the circular tube 2 again to prepare for next sampling.

It should be noted that, in order to facilitate the use of one high pressure gas source 13 for a plurality of sampling units, one main pipe is connected to the high pressure gas source 13, and the second through holes 12 connected to the high pressure gas source 13 on the plurality of sampling units can be communicated with the main pipe connected to the high pressure gas source 13 through branch pipes, so as to facilitate the supply of one gas source to the plurality of sampling units, similarly, the sterile water source 14, the clean water source 15 and the cell nutrient solution source 16 are also arranged in the same manner, and meanwhile, the push angle, the return angle and the near-rest end of the end cam 22 are smaller than the far-rest angle thereof, so as to ensure that the first through holes 10 can be communicated with the second through holes 12 on the high pressure gas source 13, the sterile water source 14, the clean water source 15 and the cell nutrient solution source 16 one by one at the far-rest end.

This device compares traditional cell culture sampling device and has following benefit:

1. this device leads to the intercommunication of groove 5 and seals through the first logical groove 3 on pipe 2 and the second on the fixed pipe 4 and realizes the sampling, it link up with the second through groove 5 to lead to the groove at first logical groove 3, the both ends of pipe 2 all do not feed through make 2 inside airtight cavitys that form of pipe make cell homodisperse to pipe 2 in under the effect of (mixing) shaft 17 and puddler 18 in order to take a sample, when first logical groove 3 and second lead to groove 5 no longer link up, both ends communicate external and high pressurized air source 13 respectively around pipe 2, make the cell culture liquid in the pipe 2 taken out, this in-process of getting cell culture liquid need not sterile environment just can realize, labour saving and time saving saves the scientific research cost simultaneously.

2. This device is accomplishing high pressurized air source 13 and taking out the back with cell culture liquid, pass through disinfection water source 14 in proper order, clear water source 15, and cell nutrient solution source 16, make 2 earlier through the disinfection water washing with the pipe of external intercommunication, wash through the clear water, the cell nutrient solution that will take out at last is carrying out supplementarily, make the nutrient solution in incubator 1 keep stable quantity, make simultaneously 2 pipes with external intercommunication again with incubator 1 intercommunication after being disinfected, whole process is high-efficient reasonable, effectively prevent personnel's maloperation.

3. When the pipe 2 of this device moved to the front side, through the cooperation of rubber disk 8 on the pipe 2 and syringe needle 7, realized that syringe needle 7 impales rubber disk 8 and realizes pipe 2 and external intercommunication, rubber disk 8 breaks away from with syringe needle 7 and realizes

rubber disk

8 and 2 front end shutoff of pipe under rubber disk 8 self elastic property during backward movement, and the effectual pollution makes whole sampling process in-process security higher in the air of having completely cut off, and the sample is safer.

4 through setting up multiunit sampling unit to multiunit sampling unit that makes is in the not co-altitude of incubator 1, can cooperate like this to realize in order to take out the cell under the different liquid level degree of depth, makes the sample process reasonable high-efficient more.

Claims

1. An aseptic sampling device for cell culture comprises a closed incubator (1) and is characterized in that a plurality of groups of sampling units are uniformly distributed in the incubator (1) at intervals from left to right in sequence, the heights of the plurality of groups of sampling units in the incubator (1) are sequentially increased from left to right, each group of sampling units comprises a circular tube (2) with two ends respectively penetrating through and extending out of the front side wall and the rear side wall of the incubator (1), the circular tubes (2) can rotate and move back and forth, an end cam (22) is sleeved and fixed at the front end of each circular tube (2), an upright column (23) capable of being matched with the end cam (22) is further arranged above each circular tube (2), the circular tubes (2) can move forwards while rotating by rotating the end cam (22), a plurality of groups of channels are arranged in parallel at intervals along the axes of the circular tubes (2) in the incubator (1), each group of channels comprises a plurality of first through grooves (3) with the circumference uniformly distributed on a round pipe (2), a fixed pipe (4) is coaxially fixed on the outer side of the part of the round pipe (2) arranged in the incubator (1), the round pipe (2) can move adherent to the inside of the fixed pipe (4), a plurality of second through grooves (5) which are in one-to-one correspondence with the first through grooves (3) are arranged on the fixed pipe (4), the front end of each round pipe (2) is fixed with an elbow (6) with one end inserted into the round pipe (2) and capable of sliding adherent to the round pipe, the other end of the elbow (6) faces downwards, one end of the elbow (6) inserted into the round pipe (2) is provided with a needle head (7), a rubber disc (8) capable of plugging the round pipe (2) is arranged in the round pipe (2), the round pipe (2) moves forwards to enable the needle head (7) to be inserted into the rubber disc (8) and enable the round pipe (2) to be communicated with a bent pipe, the rear end of the round pipe (2) is fixed with a rotary disc (9), be equipped with one on carousel (9) the outer disc first through-hole (10) with pipe (2) intercommunication, the outside coaxial arrangement of carousel (9) has one sleeve (11), sleeve (11) inner edge face and carousel (9) outer edge face adherence sliding connection, the rear side of carousel (9) is equipped with one extension spring (24), the one end of extension spring (24) is fixed with carousel (9), the other end is fixed on sleeve (11) rear end face, the front side circumference equipartition of sleeve (11) has four second through-holes (12), begin from second through-hole (12) that are located the upper right end in four second through-holes (12), high pressurized air source (13) have connected gradually clockwise, disinfection water source (14), clear water source (15) and cell nutrient solution source (16).

2. The cell culture sterile sampling device according to claim 1, wherein a stirring shaft (17) is arranged between every two adjacent sampling units, one end of the stirring shaft (17) is arranged in the incubator (1) and is circumferentially and uniformly provided with a plurality of stirring rods (18), the other end of the stirring shaft is arranged outside the incubator (1) and is coaxially fixed with a belt pulley (19), the belt pulleys (19) are connected through a belt, a first bevel gear (20) is coaxially fixed above the belt pulley (19) at the leftmost end, and a second bevel gear (21) driven by a motor is meshed above the first bevel gear (20).

3. The sterile sampling device for cell culture according to claim 1, wherein an L-shaped rod (25) is fixed at the upper end of each elbow, the vertical rod end of the L-shaped rod (25) is fixed with the elbow, and the transverse rod end is fixed on the incubator (1).

4. The sterile sampling device for cell culture according to claim 1, characterized in that the carrying boxes of the sterilized water source (14), the clear water source (15) and the cell nutrient solution source (16) are connected with pressure bottles which can provide pressure for the sterilized water source, the clear water source and the cell nutrient solution source.