CN107487862A - The preparation method of biological water purification agent - Google Patents
The preparation method of biological water purification agent Download PDFInfo
- Publication number
- CN107487862A CN107487862A CN201710642433.0A CN201710642433A CN107487862A CN 107487862 A CN107487862 A CN 107487862A CN 201710642433 A CN201710642433 A CN 201710642433A CN 107487862 A CN107487862 A CN 107487862A
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- CN
- China
- Prior art keywords
- water
- water purification
- parts
- preparation
- purification agent
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Zoology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Water Supply & Treatment (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Water Treatment By Sorption (AREA)
Abstract
The invention discloses the preparation method of biological water purification agent, including：Activation, load, extract flocculation activity material, prepare nano-sized iron oxide, compounding.Have the beneficial effect that：The biologic water cleaner can effectively remove ammonia nitrogen and nitrite in water, improve water pollution, degraded organic macromolecule, mitigate Eutrophic Extent in water, the organic macromolecule material in water body is resolved into the absorbent carbohydrate of animals and plants, amino acid, vitamin, bioactive substance etc., moreover it is possible to which hard-degraded substance is adsorbed, degraded heavy metal ion content, suppress algal grown, have stronger flocculation sediment effect to the suspension in water body, be a kind of safe and efficient biological water purification agent.
Description
Technical field
The present invention relates to water purification agent field, more particularly, to a kind of preparation method of biological water purification agent.
Technical background
The people that China has 82% drinks shallow well and river, and wherein severe water pollution bacterium exceedes accounting for for sanitary standard
75%, by the drinking water population about 1.6 hundred million of Organic Pollution.For a long time, people think that running water is safety and sanitation always
, still, because water pollution, running water of today can not be hygienic at last.One investigation display, in whole world running water
In, the chemical pollutant measured has as many as 2221 kinds, and some of which confirms as carcinogenic substance or promotees cancer thing.Mark is drunk from running water
The methods of standard is seen, Chinese to be still in reduced levels, and running water is only capable of using precipitation, filtering, chlorination, by river or underground
Water is simply processed into drinkable water.Running water chlorination can be killed effectively except germ, while can also produce more halogenated hydrocarbon compound,
The content of these chlorinated organics is multiplied, and is the maximum root for causing the mankind to suffer from various human primary gastrointestinal cancers.Water pollution is by being harmful to
Chemical substance causes the use value of water to reduce or lose, and pollutes the water of environment.Acid, alkali, oxidant in sewage, Yi Jitong,
The organic poison such as the compounds such as cadmium, mercury, arsenic, benzene, dichloroethanes, ethylene glycol, aquatile can be poisoned with poison, influence drinking water source, wind
Scenery and landscape.The oxygen in water is consumed when organic matter in sewage is decomposed by the microorganisms, the life of aquatile is influenceed, is dissolved in water
After oxygen depletion, organic matter carries out anaerobic digestion, produces the offensive gases such as hydrogen sulfide, mercaptan, water quality is further deteriorated.
Microorganism has the characteristics of individual is small, metabolism is vigorous, distribution is wide, quantity is more, also with powerful capacity of decomposition,
Their activity ensures the material circulation of the ecosystem, purifies the ecosystem, coordinates the ecological balance, is ground based on microorganism
The probiotics of hair is nontoxic, have no side effect, noresidue and secondary pollution, does not develop immunity to drugs, and can effectively improve cultivation
Ecological environment, the ecological balance is maintained, the immunity of enhancing cultivation object, reduces the generation of disease.Therefore, it is net to carry out biological water quality
Agent has higher economic value and ecological significance.
Technology on biological water purification agent has many methods, and prior art is as authorized public number
The B of CN 101935105 Chinese invention patent, discloses a kind of microorganism water treatment agent in culturing water and its preparation side
Method, the inventive method have and prepared simply by the use of the activation montmorillonite particle for being fixed with bacillus coagulans as water purification agent
Feature, but the inventive method is only using montmorillonite particle as adsorbent, using bacillus as degradation product, it is only capable of degrading organic big
Molecule, with deodorization, antibacterial, absorption ability, it can not nitrify, be photosynthetic, drop ammonia nitrogen, it is impossible to reducing heavy metal in polluted water
The content of ion, thus it is limited the effect of it purifies water, and promotional value is little.
The content of the invention
The purpose of this method is to provide a kind of preparation method of compound, efficient biological water purification agent.
Strain used in the inventive method and its bought enterprise are：Rhodopseudomonas palustris, bacillus subtilis, He Beihan
Genie et Environnement of Tang；Bacillus licheniformis, Shandong Lv Long Bioisystech Co., Ltd；Streptococcus lactis, Shandong Piao is just
Bio tech ltd；COD degradation bacterium, Guangzhou Ran Yi bio tech ltd.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：The preparation of biological water purification agent
Method, including：Activation, load, extract flocculation activity material, prepare nano-sized iron oxide, compounding, specifically including following steps：
Activation：Gangue is put into pulverizer and crushed, 35-65 mesh sieves are crossed, by colliery powder through 780-800 DEG C of calcination activation 2-
3 hours, obtain thermal activated coal gangue support samples；The strong endothermic reaction and dehydration occur in high-temperature calcination process for gangue
Reaction, and discharges certain activity, volumetric expansion after gangue is fired, its surface and is internally formed substantial amounts of micropore, together
When there is the architectural feature of micropore and mesopore, there is strong ion exchangeable and larger adsorption capacity, thermal activated coal gangue by
There is strong, renewable, the inexpensive advantage of adsorption capacity in it, can be as the good carrier of microorganism species immobilization；
Load：The accurate active coal gangue carrier for weighing 10-15 parts, is put into iodine flask, the moist heat sterilization at 120-121 DEG C
20-25 minutes, the composite bacteria liquid of 3-5 parts is added, is put into shaking table, it is small with 40-60r/min rotating speeds shake 2-2.5 at 30-33 DEG C
When, unnecessary bacterium solution is then poured out, the glutaraldehyde water solution that 40-50 parts concentration is 2-2.5% is added, 24-36 is crosslinked at 2-4 DEG C
Unnecessary glutaraldehyde is poured out after hour, with sterile water washing 4-6 times, so far composite flora immobilization is completed to produce load of microorganisms net
Water stone flour；Activated carrier mainly handles waste water by physical absorption and chemisorbed, physical absorption be mainly reflected in gangue with
Intermolecular attraction effect between contaminant molecule, this effect is by porous and the surface area decision of gangue, this absorption
Without selectivity, thus there is certain Adsorption ability to various pollutants；And chemisorbed is primarily due to gangue
In there is substantial amounts of Al, Si isoreactivity materials, can be combined with adsorbate by chemical chain, its selectivity is strong, it is generally the case that
Physical absorption and chemisorbed exist simultaneously；On the other hand it is various micro- lifes because activated carrier has flourishing specific surface area
The life and breeding of thing create good environment, are advantageous to the immobilization of composite flora；Composite flora by proliferation function and
Synergy is metabolized out polyphenoils, generates the stable ecosystem, the protozoan that makes to have Function For Purifying Water in water,
Microorganism and aquatile have recovered activity；
Extract flocculation activity material：The Moringa seed skimmed milk of 120-150 mass parts is weighed, is fitted into flask, according to solid-liquid ratio
For 1:7-8 injects distilled water in flask, and then flask is placed in 60-65 DEG C of water-bath, in the case where vacuum is 0.1-0.2kPa
Vacuum is carried out to extract 3-4 times；Filter afterwards, remove residue, filtrate is added in rotary evaporator, in vacuum 0.1-
0.2kPa, be concentrated in vacuo under the conditions of 65-70 DEG C of temperature and produces Moringa seed flocculate；Moringa seed flocculate can substantially drop
The turbidity of low muddy water, colourity, bacterial content, there is excellent flocculation；
Prepare nano-sized iron oxide：100-120 parts 0.2-0.25mol/L iron nitrate solution is taken, it is fully molten to add 30-45 part urea
Solution, solution is poured into closed glass container, is placed in water-bath or oil bath and is heated to 120-125 DEG C, reacts 2-2.5 hours,
Cooling and aging 3-4 hours are taken out, centrifuges, sediment is washed to neutrality, are then placed in drying box dry in 103-106 DEG C
Dry 12-18 hours, the presoma of rufous is obtained, the presoma is placed in Muffle furnace, 1-1.5 is calcined at 300-330 DEG C
Hour produces nano-sized iron oxide russet；Volume fraction shared by nano oxidized iron surface is big, and surface atom coordination is not complete, with
And the factor such as undersaturated dangling bonds in surface atom can improve surface-active site, make it have very strong energy of adsorption
Power, compared to traditional flocculant, it can the effective impurity such as the suspension in absorption effluent, metal, metal oxide；
Compounding：Weigh load of microorganisms water purification stone flour 20-25 parts, nano-sized iron oxide 10-15 parts, active peptides 1.5-2 parts, plant
Extract solution 3-5 parts, biology enzyme 2-4 parts, filler 30-35 parts, distilled water 45-50 parts uniform stirring at a temperature of 30-32 DEG C mix
10-15 is granulated after minute, particle diameter 2-5mm, is packed and is sealed up for safekeeping after drying；The amino acid sequence of active peptides is：
SCASRCKSRCRARRCGYYVSVYRFYRGRCYCRCLRC；Active peptides can occur with the heavy metal ion in polluted-water
Complex reaction, sedimentation is adsorbed by adsorbent so as to generate complex compound macromolecular, reduces the content of Heavy Metals in Waters ion；Should
Biologic water cleaner can effectively remove ammonia nitrogen and nitrite in water, improve water pollution, organic macromolecule of degrading, and mitigate rich in water
Nutrient laden degree, the organic macromolecule material in water body is resolved into the absorbent carbohydrate of animals and plants, amino acid, vitamin, life
Active substances etc., moreover it is possible to adsorbed to hard-degraded substance, heavy metal ion content of degrading, suppress algal grown, to water body
In suspension have stronger flocculation sediment effect, be a kind of safe and efficient biological water purification agent.
Preferably, composite bacteria liquid is Rhodopseudomonas palustris or bacillus subtilis or bacillus licheniformis or lactic acid chain
Coccus or COD degradation bacterium；Ammonia nitrogen and nitrite in water can be effectively removed, improves water pollution, organic macromolecule of degrading, is mitigated
Eutrophic Extent in water, and form microorganism overall situation with the source micropopulation in water body and purify water jointly.
Preferably, plant extraction liquid is bamboo juice or rose extract solution or olive leaf extract or said mushroom extract；
Active ingredient such as Polyphenols, flavonoids, tannin class in plant extraction liquid can effectively adsorb the stink substance in polluted-water.
Preferably, biology enzyme be amylase or protease or lipase or phosphatase or glycosidase or urase or esterase or
Cellulase；Biology enzyme can open chemical chain more complicated in water pollutant molecule, and enzyme molecule can make reactant molecule
Middle chemical bond is elongated, distorts and deformed, and makes them be easier to be hydrolyzed, thus accelerates the decomposition of organic matter, and it is degraded rapidly
For small molecule.
Preferably, filler is kaolin or bentonite or silica flour or alunite；The gap structure of filler helps to carry
The adsorption capacity of high cleanser.
Compared with prior art, the advantage of the invention is that：1）Gangue is discharged during coal mining process and coal washing
Solid waste, from gangue as microorganism carrier, the cost of water purification agent is not only reduced, and realize the complete of resource
Effect utilizes, and protects environment；2）With the heavy metal ion in polluted-water complex reaction can occur for active peptides, so as to generate
Complex compound macromolecular and be adsorbed by adsorbent sedimentation, reduce Heavy Metals in Waters ion content；3）Activated carrier has prosperity
Specific surface area, for various microorganisms life and breeding create good environment, be advantageous to the immobilization of composite flora；It is multiple
Close flora and polyphenoils is metabolized out by proliferation function and synergy, generate the stable ecosystem, make in water to have only
Protozoan, microorganism and the aquatile for changing water quality function have recovered activity；4）The biologic water cleaner can be removed effectively in water
Ammonia nitrogen and nitrite, improve water pollution, organic macromolecule of degrading, mitigate Eutrophic Extent in water, by having in water body
Machine macromolecular substances resolve into the absorbent carbohydrate of animals and plants, amino acid, vitamin, bioactive substance etc., moreover it is possible to which hardly possible is dropped
Solution material is adsorbed, and heavy metal ion content of degrading, suppresses algal grown, has stronger flocculation to sink the suspension in water body
Shallow lake acts on, and is a kind of safe and efficient biological water purification agent.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
The preparation method of biological water purification agent, comprises the following steps：
1）Gangue is put into pulverizer and crushed, crosses 35 mesh sieves, by colliery powder through 780 DEG C of calcination activations 2 hours, obtains heat
Active coal gangue support samples；2）The accurate active coal gangue carrier for weighing 10g, is put into iodine flask, damp and hot at 120 DEG C to go out
Bacterium 20 minutes, 1g streptococcus lactis, 2gCOD degradation bacterias are added, is put into shaking table, shaken 2 hours with 40r/min rotating speeds at 30 DEG C,
Then unnecessary bacterium solution is poured out, the glutaraldehyde water solution that 40g concentration is 2% is added, unnecessary penta is poured out after being crosslinked 24 hours at 2 DEG C
Dialdehyde, with sterile water washing 4 times, so far composite flora immobilization is completed to produce load of microorganisms water purification stone flour；3）Weigh 120g
Moringa seed skimmed milk, be fitted into flask, by 840g distilled water inject flask in, then flask is placed in 60 DEG C of water-baths,
Vacuum is carried out in the case where vacuum is 0.1kPa to extract 3 times；Filter afterwards, remove residue, filtrate is added in rotary evaporator,
Vacuum 0.1kPa, be concentrated in vacuo under the conditions of 65 DEG C of temperature and produces Moringa seed flocculate；Moringa seed flocculate can be bright
The aobvious turbidity, colourity, bacterial content for reducing muddy water, has excellent flocculation；4）Take 100ml 0.2mol/L nitric acid
Ferrous solution, add 30g urea and fully dissolve, solution is poured into closed glass container, be placed in water-bath or oil bath and be heated to
120 DEG C, react 2 hours, take out cooling and aging 3 hours, centrifuge, sediment is washed to neutrality, is then placed in drying box
In in 103 DEG C dry 12 hours, obtain the presoma of rufous, the presoma be placed in Muffle furnace, 1 is calcined at 300 DEG C
Hour produces nano-sized iron oxide russet；Nano-sized iron oxide can the effectively suspension in absorption effluent, metal, metal oxygen
The impurity such as compound；5）Weigh load of microorganisms water purification stone flour 20g, nano-sized iron oxide 10g, active peptides 1.5g, said mushroom extract
3g, amylase 2 g, silica flour 30g, distilled water 45g are granulated after uniform stirring mixes 10 minutes at a temperature of 30 DEG C, particle diameter
For 2mm, pack and seal up for safekeeping after drying；With the heavy metal ion in polluted-water complex reaction can occur for active peptides, so as to raw
Sedimentation is adsorbed by adsorbent into complex compound macromolecular, reduces the content of Heavy Metals in Waters ion.
Embodiment 2：
The preparation method of biological water purification agent, including：Activation, load, extraction flocculation activity material, prepare nano-sized iron oxide,
Compounding, specifically includes following steps：
1）Activation：Gangue is put into pulverizer and crushed, crosses 65 mesh sieves, by colliery powder through 800 DEG C of calcination activations 3 hours,
Obtain active coal gangue support samples；Active coal gangue can be made because it has strong, renewable, the inexpensive advantage of adsorption capacity
For the good carrier of microorganism species immobilization；
2）Load：The accurate active coal gangue carrier for weighing 15g, is put into iodine flask, moist heat sterilization 25 minutes at 121 DEG C,
2g Rhodopseudomonas palustris, 3g bacillus subtilises are added, is put into shaking table, is shaken 2.5 hours with 60r/min rotating speeds at 33 DEG C,
Then unnecessary bacterium solution is poured out, adds the glutaraldehyde water solution that 50g concentration is 2.5%, it is unnecessary to be poured out after being crosslinked 36 hours at 4 DEG C
Glutaraldehyde, with sterile water washing 6 times, so far composite flora immobilization is completed to produce load of microorganisms water purification stone flour；Composite flora
Polyphenoils is metabolized out by proliferation function and synergy, the stable ecosystem is generated, makes to purify water in water
Protozoan, microorganism and the aquatile of function have recovered activity；
3）Extract flocculation activity material：150g Moringa seed skimmed milk is weighed, is fitted into flask, 1200g distilled water is injected
In flask, then flask is placed in 65 DEG C of water-baths, carrying out vacuum in the case where vacuum is 0.2kPa extracts 4 times；Filter, remove afterwards
Remove residue, will filtrate add rotary evaporator in, under the conditions of vacuum 0.2kPa, temperature 70 C be concentrated in vacuo produce it is peppery
Wooden seed flocculate；Moringa seed flocculate can substantially reduce the turbidity, colourity, bacterial content of muddy water, have excellent wadding
Solidifying effect；
4）Prepare nano-sized iron oxide：120ml 0.25mol/L iron nitrate solution is taken, 45g urea is added and fully dissolves, by solution
Pour into closed glass container, be placed in water-bath or oil bath and be heated to 125 DEG C, react 2.5 hours, it is small to take out cooling and aging 4
When, centrifuge, sediment is washed to neutrality, be then placed in drying box and dried 18 hours in 106 DEG C, obtain rufous
Presoma, the presoma is placed in Muffle furnace, is calcined 1.5 hours at 330 DEG C and produces nano-sized iron oxide russet；Nanometer
Iron oxide can the effective impurity such as the suspension in absorption effluent, metal, metal oxide；
5）Compounding：Weigh load of microorganisms water purification stone flour 25g, nano-sized iron oxide 15g, active peptides 2g, bamboo juice 5g, lipase
2g, cellulase 2g, bentonite 35g, distilled water 50g are granulated after uniform stirring mixes 15 minutes at a temperature of 32 DEG C, particle diameter
For 5mm, pack and seal up for safekeeping after drying；The amino acid sequence of active peptides is：
SCASRCKSRCRARRCGYYVSVYRFYRGRCYCRCLRC；Active peptides can occur with the heavy metal ion in polluted-water
Complex reaction, sedimentation is adsorbed by adsorbent so as to generate complex compound macromolecular, reduces the content of Heavy Metals in Waters ion.
Embodiment 3：
The preparation method of biological water purification agent, including：Activation, load, extraction flocculation activity material, prepare nano-sized iron oxide,
Compounding, specifically includes following steps：
Activation：Gangue is put into pulverizer and crushed, crosses 50 mesh sieves, by colliery powder through 800 DEG C of calcination activations 3 hours, is obtained
Obtain thermal activated coal gangue support samples；The strong endothermic reaction and dehydration occur in high-temperature calcination process for gangue, and
Discharge certain activity, volumetric expansion after gangue is fired and is internally formed substantial amounts of micropore at its surface, while has micro-
Hole and the architectural feature of mesopore, have strong ion exchangeable and larger adsorption capacity, and thermal activated coal gangue has due to it
Strong, renewable, the inexpensive advantage of adsorption capacity, can be as the good carrier of microorganism species immobilization；
Load：The accurate active coal gangue carrier for weighing 15 parts, is put into iodine flask, moist heat sterilization 25 minutes, add at 121 DEG C
Enter 2 parts of 1 part of bacillus licheniformis, 2 parts of nisins, bacterium COD degradation bacterium, be put into shaking table, shaken at 33 DEG C with 60r/min rotating speeds
2.5 hours, unnecessary bacterium solution is then poured out, the glutaraldehyde water solution that 50 parts of concentration are 2.5% is added, after being crosslinked 36 hours at 4 DEG C
Unnecessary glutaraldehyde is poured out, with sterile water washing 6 times, so far composite flora immobilization is completed to produce load of microorganisms water purification stone flour；
Activated carrier mainly handles waste water by physical absorption and chemisorbed, and physical absorption is mainly reflected in gangue and pollutant point
Intermolecular attraction effect between son, this effect are determined that this absorption does not select by the porous and surface area of gangue
Property, thus have certain Adsorption ability to various pollutants；And chemisorbed be primarily due in gangue there is
Substantial amounts of Al, Si isoreactivity material, it can be combined with adsorbate by chemical chain, its selectivity is strong, it is generally the case that physical absorption
Exist simultaneously with chemisorbed；On the other hand it is the life of various microorganisms because activated carrier has flourishing specific surface area
Good environment is created with breeding, is advantageous to the immobilization of composite flora；Composite flora passes through proliferation function and synergy
Be metabolized out polyphenoils, generate the stable ecosystem, the protozoan that makes to have Function For Purifying Water in water, microorganism and
Aquatile has recovered activity；
Extract flocculation activity material：The Moringa seed skimmed milk of 150 mass parts is weighed, is fitted into flask, is 1 according to solid-liquid ratio:8
Distilled water is injected in flask, then flask is placed in 65 DEG C of water-baths, vacuum extraction 4 is carried out in the case where vacuum is 0.2kPa
It is secondary；Filter afterwards, remove residue, filtrate is added in rotary evaporator, carried out under the conditions of vacuum 0.2kPa, temperature 70 C
It is concentrated in vacuo and produces Moringa seed flocculate；The turbidity, colourity, bacterium that Moringa seed flocculate can substantially reduce muddy water contain
Amount, has excellent flocculation；
Prepare nano-sized iron oxide：120 parts of 0.25mol/L iron nitrate solution is taken, 40 parts of urea is added and fully dissolves, solution is fallen
Enter in closed glass container, be placed in water-bath or oil bath and be heated to 120 DEG C, react 2 hours, take out cooling and aging 3 hours, from
The heart is separated, and sediment is washed into neutrality, is then placed in drying box and is dried 15 hours in 103 DEG C, obtains the forerunner of rufous
Body, the presoma is placed in Muffle furnace, is calcined 1 hour at 300 DEG C and produces nano-sized iron oxide russet；Nano-sized iron oxide
Volume fraction shared by surface is big, and surface atom coordination is not complete, and the factor such as undersaturated dangling bonds in surface atom can
To improve surface-active site, very strong adsorption capacity is made it have, compared to traditional flocculant, it can effectively adsorb dirt
The impurity such as suspension, metal, metal oxide in water；
Compounding：Weigh 20 parts of load of microorganisms water purification stone flour, 12 parts of nano-sized iron oxide, 1.5 parts of active peptides, 2 parts of bamboo juice, olive
2 parts of olive leaf extract, 1 part of lipase, 2 parts of urase, 30 parts of kaolin, 45 parts of the distilled water uniform stirring at a temperature of 30 DEG C mix
It is granulated after 12 minutes, particle diameter 4mm, packs and seal up for safekeeping after drying；The amino acid sequence of active peptides is：
SCASRCKSRCRARRCGYYVSVYRFYRGRCYCRCLRC；Active peptides can occur with the heavy metal ion in polluted-water
Complex reaction, sedimentation is adsorbed by adsorbent so as to generate complex compound macromolecular, reduces the content of Heavy Metals in Waters ion；Should
Biologic water cleaner can effectively remove ammonia nitrogen and nitrite in water, improve water pollution, organic macromolecule of degrading, and mitigate rich in water
Nutrient laden degree, the organic macromolecule material in water body is resolved into the absorbent carbohydrate of animals and plants, amino acid, vitamin, life
Active substances etc., moreover it is possible to adsorbed to hard-degraded substance, heavy metal ion content of degrading, suppress algal grown, to water body
In suspension have stronger flocculation sediment effect, be a kind of safe and efficient biological water purification agent.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Lanxi City Pu Run Si aquaculture technologies Co., Ltd
<120>The preparation method of biological water purification agent
<130> 2
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 36
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Gly
1 5 10 15
Tyr Tyr Val Ser Val Tyr Arg Phe Tyr Arg Gly Arg Cys Tyr Cys Arg
20 25 30
Cys Leu Arg Cys
35
Claims (8)
1. the preparation method of biological water purification agent, including：Activation, load, extraction flocculation activity material, prepare it is nano oxidized
Iron, compounding, it is characterised in that：Described compounding step is more to weigh load of microorganisms water purification stone flour, nano-sized iron oxide, activity
Peptide, plant extraction liquid, biology enzyme, filler, distilled water are made after uniform stirring mixing 10-15 minutes at a temperature of 30-32 DEG C
Grain, particle diameter 2-5mm, pack and seal up for safekeeping after drying.
2. the preparation method of biological water purification agent according to claim 1, it is characterised in that：Described activation step
For：Gangue is put into pulverizer and crushed, crosses 35-65 mesh sieves, by colliery powder through 780-800 DEG C of calcination activation 2-3 hour,
Obtain thermal activated coal gangue support samples.
3. the preparation method of biological water purification agent according to claim 1, it is characterised in that：Described load step
For：The accurate active coal gangue carrier for weighing 10-15 parts, is put into iodine flask, moist heat sterilization 20-25 points at 120-121 DEG C
Clock, the composite bacteria liquid of 3-5 parts is added, is put into shaking table, 2-2.5 hours, Ran Houqing are shaken with 40-60r/min rotating speeds at 30-33 DEG C
Go out unnecessary bacterium solution, add the glutaraldehyde water solution that 40-50 parts concentration is 2-2.5%, poured out after being crosslinked 24-36 hours at 2-4 DEG C
Unnecessary glutaraldehyde, with sterile water washing 4-6 times, so far composite flora immobilization is completed to produce load of microorganisms water purification stone flour.
4. the preparation method of biological water purification agent according to claim 1, it is characterised in that：In described load step
Composite bacteria liquid be：Rhodopseudomonas palustris or bacillus subtilis or bacillus licheniformis or streptococcus lactis or COD degradation
Bacterium.
5. the preparation method of biological water purification agent according to claim 1, it is characterised in that：Described extraction flocculation is lived
Property material step is：The Moringa seed skimmed milk of 120-150 mass parts is weighed, is fitted into flask, is 1 according to solid-liquid ratio:7-8 will
In distilled water injection flask, then flask is placed in 60-65 DEG C of water-bath, Vaccum Permeating is carried out in the case where vacuum is 0.1-0.2kPa
Carry 3-4 times；Filter afterwards, remove residue, filtrate is added in rotary evaporator, in vacuum 0.1-0.2kPa, temperature 65-70
Carry out being concentrated in vacuo under the conditions of DEG C and produce Moringa seed flocculation activity material.
6. the preparation method of biological water purification agent according to claim 1, it is characterised in that：Described preparation nano oxygen
Changing iron step is：100-120 parts 0.2-0.25mol/L iron nitrate solution is taken, 30-45 part urea is added and fully dissolves, will be molten
Liquid is poured into closed glass container, is placed in water-bath or oil bath and is heated to 120-125 DEG C, reacts 2-2.5 hours, takes out cooling
3-4 hours are aged, centrifuges, sediment is washed to neutrality, is then placed in drying box in 103-106 DEG C of dry 12-18
Hour, obtain the presoma of rufous, the presoma be placed in Muffle furnace, at 300-330 DEG C be calcined 1-1.5 hours produce
Nano-sized iron oxide russet.
7. the preparation method of biological water purification agent according to claim 1, it is characterised in that：Described compounding step
For：Weigh load of microorganisms water purification stone flour 20-25 parts, nano-sized iron oxide 10-15 parts, active peptides 1.5-2 parts, plant extraction liquid
3-5 parts, biology enzyme 2-4 parts, filler 30-35 parts, distilled water 45-50 parts are uniform stirring mixing 10-15 points at a temperature of 30-32 DEG C
It is granulated after clock, particle diameter 2-5mm, packs and seal up for safekeeping after drying.
8. the preparation method of biological water purification agent according to claim 1, it is characterised in that：In described compounding step
The amino acid sequences of active peptides be：SCASRCKSRCRARRCGYYVSVYRFYRGRCYCRCLRC；
The preparation method of biological water purification agent according to claim 1, it is characterised in that：In described compounding step
Plant extraction liquid is bamboo juice or rose extract solution or olive leaf extract or said mushroom extract：Biology enzyme is amylase or egg
White enzyme or lipase or phosphatase or glycosidase or urase or esterase or cellulase：Filler is kaolin or bentonite or quartz
Powder or alunite.
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Cited By (2)
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| CN113277627A (en) * | 2021-04-19 | 2021-08-20 | 广西壮族自治区环境保护科学研究院 | Method for treating and recycling urban black and odorous water sludge |
| CN114105314A (en) * | 2021-12-09 | 2022-03-01 | 苏州中晟环境修复有限公司 | Water environment restoration material and preparation method thereof |
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| CN113277627A (en) * | 2021-04-19 | 2021-08-20 | 广西壮族自治区环境保护科学研究院 | Method for treating and recycling urban black and odorous water sludge |
| CN113277627B (en) * | 2021-04-19 | 2022-10-04 | 广西壮族自治区环境保护科学研究院 | Method for treating and recycling urban black and odorous water sludge |
| CN114105314A (en) * | 2021-12-09 | 2022-03-01 | 苏州中晟环境修复有限公司 | Water environment restoration material and preparation method thereof |
| CN114105314B (en) * | 2021-12-09 | 2023-12-15 | 苏州中晟环境修复有限公司 | Water environment restoration material and preparation method thereof |
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Application publication date: 20171219 |