CN106053197B - Resonance blending system and method - Google Patents
Resonance blending system and method Download PDFInfo
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- CN106053197B CN106053197B CN201610345071.4A CN201610345071A CN106053197B CN 106053197 B CN106053197 B CN 106053197B CN 201610345071 A CN201610345071 A CN 201610345071A CN 106053197 B CN106053197 B CN 106053197B
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- 238000002156 mixing Methods 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000012360 testing method Methods 0.000 claims abstract description 153
- 238000004321 preservation Methods 0.000 claims abstract description 62
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 52
- 238000012790 confirmation Methods 0.000 claims abstract description 19
- 239000000919 ceramic Substances 0.000 claims description 33
- 238000009413 insulation Methods 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims 1
- 238000003756 stirring Methods 0.000 abstract description 11
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 238000010586 diagram Methods 0.000 description 10
- 230000005540 biological transmission Effects 0.000 description 4
- 230000015271 coagulation Effects 0.000 description 4
- 238000005345 coagulation Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 230000036760 body temperature Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000003593 chromogenic compound Substances 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000004898 kneading Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000013522 software testing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
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- Immunology (AREA)
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- Automatic Analysis And Handling Materials Therefor (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The application provides a resonance blending system and a method, and the system comprises: the automatic cup feeding device, the sample feeding device and the resonance device are connected with the automatic controller, and the heat preservation device is connected with the resonance device; the automatic controller is used for controlling the cup supplying device to add the test cup to the heat preservation device; when a first confirmation instruction of the cup supplying device is received to determine that the test cup is placed completely, controlling the sample adding device to add a sample and a reagent into the test cup; when a second confirmation instruction of the sample adding device is received to determine that the sample adding is finished, driving the resonance device to resonate; the resonance device is used for generating resonance force and transmitting the resonance force to the heat preservation device; and the heat preservation device is used for driving the test cup to resonate under the action of the resonant force and uniformly mixing the sample and the reagent in the test cup. The application can not produce the stirring dead angle, can not destroy the structure of macromolecular enzyme, can guarantee the uniformity of test result through the sample and the reagent in the resonance force abundant mixing test cup that resonance device produced.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to a resonance blending system and method.
Background
In a blood coagulation tester, a test cup added with a sample and a reagent needs to be mixed uniformly, and then the mixed liquid is tested. In the prior art, a sample and a reagent in a test cup are generally mixed uniformly by using an umbrella-paddle stirring type or an ultrasonic type, however, the umbrella-paddle stirring type cannot mix the stirring dead angle in a rectangular test cup uniformly, so that the consistency and the stability of a test result are influenced; the ultrasonic method is extremely destructive to the structure of macromolecular enzyme in a sample and a reagent, and can also influence the consistency and stability of test results.
Disclosure of Invention
In view of this, the present application provides a resonance blending system and a method thereof, so as to solve the problem that the consistency and stability of the test result are affected by the existing umbrella paddle stirring type or ultrasonic type.
According to a first aspect of an embodiment of the present application, there is provided a resonant blending system, the system including: the automatic cup feeding device, the sample feeding device and the resonance device are respectively connected with the automatic controller, and the heat preservation device is connected with the resonance device;
the automatic controller is used for controlling the cup supplying device to add a test cup into the heat preservation device; when the first confirmation instruction of the cup supplying device is received to determine that the test cup is placed completely, controlling the sample adding device to add a sample and a reagent into the test cup; when a second confirmation instruction of the sample adding device is received to determine that the sample adding is finished, driving the resonance device to resonate;
the resonance device is used for generating resonance force and transmitting the resonance force to the heat preservation device;
the heat preservation device is used for driving the test cup to resonate under the action of the resonant force and uniformly mixing the sample and the reagent in the test cup.
According to a second aspect of the embodiments of the present application, there is provided a resonance blending method, including:
the automatic controller controls the cup supplying device to add the test cup into the heat preservation device;
when the automatic controller receives a first confirmation instruction of the cup supply device and determines that the test cup is placed completely, the automatic controller controls the sample adding device to add a sample and a reagent into the test cup;
when the automatic controller receives a second confirmation instruction of the sample adding device and determines that the sample adding is finished, the automatic controller drives the resonance device to resonate;
generating a resonant force by the resonant device and transmitting the resonant force to the thermal insulation device;
the heat preservation device drives the test cup to resonate under the action of the resonant force, and the sample and the reagent in the test cup are uniformly mixed.
By applying the embodiment of the application, the resonance device is driven to resonate by the automatic controller, then the resonance device generates resonance force and transmits the resonance force to the heat preservation device, the heat preservation device drives the test cup to resonate under the action of the resonance force, and the sample and the reagent in the test cup are uniformly mixed.
Drawings
FIG. 1A is a block diagram of a resonant blending system according to an exemplary embodiment of the present application;
FIG. 1B is a top view of the thermal insulation and resonance device of the system of FIG. 1A;
FIG. 1C is a cross-sectional view taken along line B-B of the top view structural diagram shown in FIG. 1B;
FIG. 1D is a schematic diagram of the eccentric wheel motor in the embodiment of FIG. 1C generating resonant forces in all directions;
FIG. 1E is a perspective view of the insulation and resonator devices of the embodiment shown in FIGS. 1B and 1C;
FIG. 2A is a cross-sectional block diagram of a resonating device illustrated herein in accordance with an exemplary embodiment;
FIG. 2B is a cross-sectional perspective view of the resonating device in the embodiment shown in FIG. 2A;
FIG. 3 is a flow chart illustrating a method of resonant blending according to an exemplary embodiment of the present application;
FIG. 4 is a flow diagram illustrating another method of resonant blending according to an exemplary embodiment of the present application;
fig. 5 is a flow chart illustrating another resonant blending method according to an exemplary embodiment of the present application.
Detailed Description
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present application. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present application, as detailed in the appended claims.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this application and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.
It is to be understood that although the terms first, second, third, etc. may be used herein to describe various information, such information should not be limited to these terms. These terms are only used to distinguish one type of information from another. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present application. The word "if" as used herein may be interpreted as "at … …" or "when … …" or "in response to a determination", depending on the context.
Fig. 1A is a block diagram of a resonant blending system according to an exemplary embodiment of the present application, as shown in fig. 1A, the system including: the automatic cup feeding device comprises an automatic controller 11, a cup feeding device 12, a sample adding device 13 and a resonance device 14 which are respectively connected with the automatic controller 11, and a heat preservation device 15 connected with the resonance device 14.
Wherein, the automatic controller 11 is used for controlling the cup supplying device 12 to add the test cup into the heat preservation device 15; when the first confirmation instruction of the cup supply device 12 is received to determine that the placement of the test cup is finished, controlling the sample adding device 13 to add the sample and the reagent into the test cup; when the second confirmation instruction of the sample adding device 13 is received to determine that the sample adding is finished, the resonance device 14 is driven to resonate;
a resonance device 14 for generating and transmitting a resonance force to the temperature keeping device 15;
and the heat preservation device 15 is used for driving the test cup to resonate under the action of the resonant force and uniformly mixing the sample and the reagent in the test cup.
In an embodiment, the automatic controller 11 can be further configured to send a cup moving instruction to the cup feeding device 12 and send a sample adding instruction to the sample adding device 13; the cup feeding device 12 can also be used for detecting whether the uniformly mixed test cup is still placed in the heat preservation device 15 when a cup moving instruction is received; if the uniformly mixed test cup is still placed in the heat preservation device 15, the uniformly mixed test cup is grabbed from the heat preservation device 15, and the uniformly mixed test cup is placed on the conveying device; grabbing an empty test cup from the test cup containing structure, and placing the empty test cup in a cup groove structure of the heat preservation device 15; the sample adding device 13 can also be used for adding samples and reagents into the empty test cup when receiving a sample adding instruction.
Wherein, supply including microprocessor in the cup device 12, grab the cup structure, hold the test cup structure, microprocessor is when receiving the cup instruction that moves that automatic control 11 sent, whether still placed the test cup of mixing in the detection heat preservation device 15, if still placed the test cup of mixing in the heat preservation device 15, microprocessor control is grabbed the cup structure and is grabbed the test cup of mixing, and place the test cup of mixing on transmission device, should grab the cup structure and snatch empty test cup from holding the test cup structure, and place empty test cup in heat preservation device 15's cup groove structure.
Fig. 1B is a top view structural diagram of the heat preservation device and the resonance device in the system shown in fig. 1A, fig. 1C is a cross-sectional structural diagram taken along line B-B in the top view structural diagram shown in fig. 1B, and as shown in fig. 1B and fig. 1C, the heat preservation device 15 may also be referred to as a sample incubation device and is used for simulating a human body temperature environment, for example, 37 degrees celsius, the heat preservation device 15 may further include a temperature control circuit and a heat preservation layer 152 in addition to a cup structure 151 (including a plurality of cup structures) for holding a test cup, the heat preservation layer has a low specific heat capacity and good rigidity, and the temperature control circuit is used for controlling the temperature of the heat preservation device 15 to be maintained at 37 degrees celsius.
In an embodiment, as shown in fig. 1C, the resonance device 14 may include a vibration motor 141, and a linear slide 142, where the vibration motor 141 may be configured to receive a first voltage signal sent by the automatic controller 11, start to rotate under the driving of the first voltage signal, and generate resonance forces in various directions, and the linear slide 142 may be configured to absorb and filter the resonance forces in various directions and obtain a resonance force axially parallel to the linear slide 142. In addition, the resonance device 14 may further include a vibration base 143, two symmetrical ends of the vibration base 143 are respectively connected to the vibration motor 141 and the linear slide rail 142, and the other end of the vibration base 143 is connected to the thermal insulation device 15, so as to transmit the resonance force axially parallel to the linear slide rail 142 to the thermal insulation device 15.
Wherein, the vibration motor 141 can be a high-speed eccentric wheel motor, and under the driving of the first voltage signal, the vibration motor starts to rotate to generate resonance force in each direction, fig. 1D is a schematic diagram of the eccentric wheel motor in the embodiment shown in fig. 1C generating resonance force in each direction, and as shown in fig. 1D, the resonance frequency generated by the high-speed eccentric wheel motor can be 3KHz to 5KHz, so as to ensure that the sample and the reagent in the test cup can be fully mixed, and the structure of the macromolecular enzyme cannot be damaged. Because the two symmetrical ends of the vibration base 143 are respectively connected with the vibration motor 141 and the linear slide rail 142, and the other end of the vibration base 143 is connected with the heat insulation layer 152 of the heat insulation device 15, the resonance force in each direction generated by the vibration motor 141 can be filtered and absorbed by the linear slide rail 142, the resonance force axially parallel to the linear slide rail 142 is finally obtained, and the resonance force axially parallel to the linear slide rail 142 is transmitted to the heat insulation device 15 through the vibration base 143, the heat insulation device 15 drives the test cup to resonate under the action of the resonance force, and the sample and the reagent in the test cup 5 are uniformly mixed.
In an embodiment, fig. 1E is a perspective structural view of the insulation device and the resonance device in the embodiments shown in fig. 1B and fig. 1C, as shown in fig. 1E, the resonance blending system may further include springs 16 respectively connected to two symmetrical ends of the resonance device 14, and the automatic controller 11 may include a first determining unit for determining a duration of the resonance device 14; a control unit for stopping driving the resonance device 14 when the duration determined by the first determining unit reaches a first preset duration, stopping resonance of the test cup by the elastic force of the spring 16 and resetting the test cup; and a second determining unit for determining a time point corresponding to the stop of driving the resonance device 14, and continuing to perform the step of controlling the cup supplying device 12 to add the test cup to the temperature keeping device 15 after a second preset time period from the time point determined by the second determining unit.
Specifically, the automatic controller 11 determines a duration of driving the vibration motor 141 to vibrate, and when the duration reaches a first preset duration, the automatic controller 11 controls to stop driving the vibration motor 141; under the elastic force of the springs 16 positioned at both sides of the resonance device 14, the test cup stops resonance and is reset; the corresponding time point for stopping driving the vibration motor 141 is determined again by the automatic controller 11; after a second preset time period from the time point, the step of the automatic controller 11 sending the cup moving command to the cup feeding device 12 is continuously executed.
The first preset time may be in a range of 1 to 10 seconds, the spring 16 may include a plurality of disc springs, the disc springs have the same shape, the same stiffness coefficient, and the same elastic modulus, and may be assembled into two disc spring sets in an involution manner, and after applying a certain pretightening force, the two disc spring sets are respectively installed on two sides of the vibration base 143 in the resonance device 14, and when the automatic controller 11 stops driving the vibration motor 141, and after a second preset time, the test cup stops resonance and resets, and the second preset time may be in a range of 5 to 10 milliseconds.
According to the embodiment, the automatic controller 11 drives the resonance device 14 to resonate, the resonance device 14 generates resonance force and transmits the resonance force to the heat preservation device 15, the heat preservation device 15 drives the test cup to resonate under the action of the resonance force, and samples and reagents in the test cup are uniformly mixed.
In an embodiment, fig. 2A is a cross-sectional structural view of a resonance device shown in the present application according to an exemplary embodiment, fig. 2B is a cross-sectional perspective structural view of the resonance device in the embodiment shown in fig. 2A, and the resonance device 14 may include a piezoelectric ceramic oscillator 144, configured to receive a second voltage signal sent by the automatic controller 11, and start to vibrate and generate a resonance force parallel to a central axis of the piezoelectric ceramic oscillator 144 under the driving of the second voltage signal; the resonance device can further comprise a vibration plate 145, the vibration plate 145 is provided with a cup groove structure 146, and the vibration plate 145 is used for receiving a resonance force which is transmitted by the piezoelectric ceramic oscillator 144 and is parallel to the central axis of the piezoelectric ceramic oscillator 144, driving the test cup in the cup groove structure 146 to resonate under the action of the resonance force, and uniformly mixing the sample and the reagent in the test cup.
The piezoelectric ceramic oscillator 144 is cylindrical, and starts to vibrate and generate a resonant force parallel to the central axis of the piezoelectric ceramic oscillator 144 under the driving of a second voltage signal sent by the automatic controller 11, wherein the resonant frequency of the resonant force can be 3KHz to 5KHz, so as to ensure that the sample and the reagent in the test cup can be fully mixed, and the structure of the macromolecular enzyme cannot be damaged.
In the present embodiment, the temperature environment of the test cup in the cup groove structure 146 of the vibration plate 145 may be maintained by a heat radiation device, which is used to simulate the human body temperature environment, for example, the heat radiation device is maintained at 37 degrees celsius.
In one embodiment, as shown in fig. 2A and 2B, the resonant kneading system may further include plate springs 17 connected to both symmetrical sides of the vibration plate 145. Because the piezoelectric ceramic oscillator is connected with the plate spring 17 on one side of the vibrating plate 145, the piezoelectric ceramic oscillator 144 can transmit the resonance force parallel to the central axis of the piezoelectric ceramic oscillator 144 to the vibrating plate 145 through the plate spring 17, the vibrating plate 145 drives the test cup in the cup groove structure 146 to resonate under the action of the resonance force, and the sample and the reagent in the test cup are uniformly mixed so that the test device can test the uniformly mixed liquid.
Further, the automatic controller 11 determines the duration of the vibration of the driving piezoelectric ceramic oscillator 144; when the duration reaches a first preset duration, the automatic controller 11 controls to stop driving the piezoelectric ceramic oscillator 144; under the elastic force of the plate springs 17 positioned at both sides of the vibration plate 145, the test cup stops resonating and is reset; the corresponding time point for stopping driving the piezoelectric ceramic oscillator 144 is determined again by the automatic controller 11; after a second preset time period from the time point, the step of the automatic controller 11 sending the cup moving command to the cup feeding device 12 is continuously executed.
According to the above embodiment, the piezoelectric ceramic oscillator 144 in the resonance device 14 is driven by the automatic controller 11 to vibrate and generate a resonance force parallel to the central axis of the piezoelectric ceramic oscillator 144, and after the piezoelectric ceramic oscillator 144 transmits the resonance force to the vibration plate 145 in the resonance device 14, the vibration plate 145 drives the test cup in the cup-and-groove structure 146 to resonate under the resonance force, so as to mix the sample and the reagent in the test cup. Based on above-mentioned implementation, can fully mix the sample and the reagent in the test cup through the resonance force that resonance device 14 produced, need not the stirring rod stirring mixing sample and reagent, can avoid cross contamination to no matter what shape test cup, all can not appear stirring dead angle at the mixing in-process, the resonance mixing can not destroy macromolecular enzyme's structure yet simultaneously, thereby can guarantee the uniformity and the stability of test result.
Fig. 3 is a flowchart illustrating an embodiment of a resonant blending method according to an exemplary embodiment of the present application, where as shown in fig. 3, the embodiment includes the following steps:
step 301: the automatic controller controls the cup supplying device to add the test cup into the heat preservation device;
step 302: when the automatic controller receives a first confirmation instruction of the cup supply device to determine that the test cup is placed completely, the automatic controller controls the sample adding device to add a sample and a reagent into the test cup;
step 303: when the automatic controller receives a second confirmation instruction of the sample adding device to determine that the sample adding is finished, the automatic controller drives the resonance device to resonate;
step 304: generating resonance force through the resonance device and transmitting the resonance force to the heat preservation device;
step 305: the heat preservation device drives the test cup to resonate under the action of the resonant force, and the sample and the reagent in the test cup are uniformly mixed.
The flow described in step 301 to step 305 may refer to the related description of the embodiment shown in fig. 1A, and is not repeated.
In the embodiment, the resonance device is driven by the automatic controller to resonate, the resonance device generates resonance force and transmits the resonance force to the heat preservation device, the heat preservation device drives the test cup to resonate under the action of the resonance force, and the samples and the reagents in the test cup are uniformly mixed.
Fig. 4 is a flowchart illustrating another embodiment of a resonant blending method according to an exemplary embodiment of the present application, where as shown in fig. 4, the embodiment includes the following steps:
step 401: the automatic controller controls the cup supplying device to add the test cup into the heat preservation device;
automatic control can send the cup instruction of moving to supplying the cup device, supplies the cup device when receiving this cup instruction of moving, whether still placed the test cup of mixing among the detection heat preservation device, if still placed the test cup of mixing among the heat preservation device, snatch the test cup of mixing from the heat preservation device through supplying the cup device, and place the test cup of mixing on transmission device, then supply the cup device to snatch empty test cup from holding the test cup structure, place empty test cup in heat preservation device's cup groove structure.
Step 402: when the automatic controller receives a first confirmation instruction of the cup supply device to determine that the test cup is placed completely, the automatic controller controls the sample adding device to add a sample and a reagent into the test cup;
the steps 401 and 402 are as described in steps 301 and 302, and are not described again.
The sample may be plasma, the reagent may be a reagent if the coagulation tester uses a chromogenic substrate method for the coagulation test, and the reagent may be an intermediate reagent if the coagulation tester uses an immunoturbidimetric method for the coagulation test.
Step 403: when the automatic controller receives a second confirmation instruction of the sample adding device and determines that the sample adding is finished, sending a first voltage signal to a vibration motor arranged in a resonance device through the automatic controller, and driving the vibration motor to start rotating and generate resonance force in each direction through the first voltage signal;
the sample adding device sends a second confirmation instruction to the automatic controller after adding the sample and the reagent into the empty test cup, the automatic controller determines that the sample adding is finished, sends a first voltage signal to a vibration motor arranged in the resonance device, and drives the vibration motor to rotate through the first voltage signal to generate resonance force in each direction.
Step 404: absorbing and filtering the resonance force in each direction through a linear slide rail arranged in the resonance device, and obtaining the resonance force parallel to the axial direction of the linear slide rail;
step 405: the vibration base body arranged in the resonance device transmits the resonance force parallel to the axial direction of the linear slide rail to the heat preservation device;
step 406: the heat preservation device drives the test cup to resonate under the action of the resonant force, and the sample and the reagent in the test cup are uniformly mixed.
The flow from step 403 to step 406 may refer to the related descriptions of the embodiments shown in fig. 1B, fig. 1C, fig. 1D, and fig. 1E, and will not be described again.
According to the embodiment, the automatic controller drives the resonance device to resonate, the resonance device generates resonance force and transmits the resonance force to the heat preservation device, the heat preservation device drives the test cup to resonate under the action of the resonance force, and the samples and the reagents in the test cup are uniformly mixed.
Fig. 5 is a flowchart of another embodiment of a resonance blending method according to an exemplary embodiment of the present application, in which, after blending in the previous embodiment, a sample adding device further adds an activating reagent or a color generating agent to a blended test cup, where the activating reagent corresponds to the immunoturbidimetry method mentioned in the embodiment shown in fig. 4, and the color generating agent corresponds to the chromogenic substrate method mentioned in the embodiment shown in fig. 4, and the blended test cup can be re-blended by the blending method in this embodiment, so that the testing device can test the re-blended liquid in the test cup, as shown in fig. 5, the embodiment includes the following steps:
step 501: controlling the cup feeding device to add a test cup into a cup groove structure of a vibrating plate arranged in the resonance device through the automatic controller;
step 501 is as described in step 401, which is not repeated, but in this embodiment, the cup supplying device grabs the test cup that has been mixed once from the conveying device, the sample adding device adds a start reagent or a color producing reagent to the test cup, and after the cup supplying device grabs the test cup that has been mixed once, the test cup that has been mixed once is placed in the cup slot structure of the vibrating plate.
Step 502: when the automatic controller receives a first confirmation instruction of the cup supplying device to determine that the test cup is placed completely, a second voltage signal is sent to a piezoelectric ceramic oscillator arranged in the resonance device through the automatic controller, the piezoelectric ceramic oscillator is driven to start vibrating through the second voltage signal, and a resonance force parallel to the central axis of the piezoelectric ceramic oscillator is generated;
step 503: transmitting a resonance force parallel to a central axis of the piezoelectric ceramic oscillator to a vibration plate provided in a resonance device through the piezoelectric ceramic oscillator;
step 504: the vibration plate drives the test cup positioned in the cup groove structure to resonate under the action of resonance force, and the sample and the reagent in the test cup are uniformly mixed.
The flow from step 502 to step 504 may refer to the related description of the embodiment shown in fig. 2A and fig. 2B, and is not described again.
According to the embodiment, the piezoelectric ceramic oscillator in the resonance device is driven by the automatic controller to vibrate and generate the resonance force parallel to the central axis of the piezoelectric ceramic oscillator, and after the piezoelectric ceramic oscillator transmits the resonance force to the vibration plate in the resonance device, the vibration plate drives the test cup in the cup slot structure to resonate under the action of the resonance force, so that the sample and the reagent in the test cup are uniformly mixed. Based on above-mentioned implementation, can be with the abundant mixing of sample and reagent in the test cup through the resonance force that resonance device produced, need not stirring rod stirring mixing sample and reagent, can avoid cross contamination to no matter what shape's test cup, all can not appear stirring dead angle at the mixing in-process, the resonance mixing can not destroy macromolecular enzyme's structure yet simultaneously, thereby can guarantee the uniformity and the stability of test result. In addition, the in-process of resonance device at resonance mixing test cup, the application of sample device with supply the cup device incessant, the application of sample device can continue to add reagent for the test cup on the transmission device, supply the cup device can continue to place the test cup that has mixed once on transmission device to the efficiency of software testing of blood coagulation tester has been improved.
The automatic controller in the embodiment of the application can be realized by a hardware circuit according to the design requirement of an actual circuit. It can be clearly understood by those skilled in the art that, for convenience and brevity of description, the specific working process of the method embodiment described above may refer to the corresponding process in the foregoing system embodiment, and is not described herein again.
Those of ordinary skill in the art will understand that: all or part of the steps for implementing the embodiments can be implemented by hardware related to program instructions, and the program can be stored in a computer readable storage medium, and when executed, the program performs the steps including the method embodiments; and the aforementioned storage medium includes: various media that can store program codes, such as ROM, RAM, magnetic or optical disks.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions in the embodiments of the present application.
Claims (6)
1. A resonant blending system, comprising: the device comprises an automatic controller, a cup feeding device, a sample adding device, a resonance device and a heat preservation device, wherein the cup feeding device, the sample adding device and the resonance device are respectively connected with the automatic controller;
the automatic controller is used for controlling the cup supplying device to add a test cup into the heat preservation device; when the first confirmation instruction of the cup supplying device is received to determine that the test cup is placed completely, controlling the sample adding device to add a sample and a reagent into the test cup; when a second confirmation instruction of the sample adding device is received to determine that the sample adding is finished, driving the resonance device to resonate;
the first resonance device is used for generating resonance force and transmitting the resonance force to the heat preservation device;
the heat preservation device is used for driving the test cup to resonate under the action of the resonant force and uniformly mixing the sample and the reagent in the test cup;
the first resonance device includes:
the vibration motor is used for receiving a first voltage signal sent by the automatic controller, starts to rotate under the drive of the first voltage signal and generates resonant force in each direction, the vibration motor is a high-speed eccentric wheel motor, and the frequency of the generated resonant force is 3KHz-5 KHz;
the linear sliding rail is used for absorbing and filtering the resonance force in each direction and obtaining the resonance force which is axially parallel to the linear sliding rail;
the vibration base body is used for transmitting the resonance force axially parallel to the linear slide rail to the heat preservation device;
the second resonance device includes: a piezoelectric ceramic oscillator;
the piezoelectric ceramic oscillator is used for receiving a second voltage signal sent by the automatic controller, starts to vibrate under the driving of the second voltage signal and generates a resonance force parallel to the central axis of the piezoelectric ceramic oscillator;
the second resonance device further includes: the vibrating plate is provided with a cup groove structure;
the vibration plate is used for receiving a resonance force which is transmitted by the piezoelectric ceramic oscillator and is parallel to the central axis of the piezoelectric ceramic oscillator, driving the test cup in the cup groove structure to resonate under the action of the resonance force, and uniformly mixing the sample and the reagent in the test cup.
2. The system of claim 1,
the automatic controller is also used for sending a cup moving instruction to the cup supplying device and sending a sample adding instruction to the sample adding device;
the cup supplying device is also used for detecting whether the uniformly mixed test cup is still placed in the heat preservation device when the cup moving instruction is received; if the uniformly mixed test cup is still placed in the heat preservation device, grabbing the uniformly mixed test cup from the heat preservation device, and placing the uniformly mixed test cup on a conveying device; grabbing an empty test cup from the test cup containing structure, and placing the empty test cup in a cup groove structure of the heat preservation device;
and the sample adding device is also used for adding a sample and a reagent into the empty test cup when the sample adding instruction is received.
3. The system of claim 1, further comprising springs connected to symmetrical ends of the first resonating means, respectively; the automatic controller includes:
a first determination unit for determining a duration of driving the first resonance device to resonate;
the control unit is used for stopping driving the first resonance device when the duration determined by the first determination unit reaches a first preset duration, and stopping resonating and resetting the test cup through the elastic force of the spring;
and the second determining unit is used for determining a time point corresponding to the stop of driving the first resonance device, and continuously executing the step of controlling the cup supplying device to add the test cup into the heat preservation device after a second preset time period from the time point determined by the second determining unit.
4. A resonance blending method capable of realizing the system of any one of claims 1 to 3, characterized by comprising:
the automatic controller controls the cup supplying device to add the test cup into the heat preservation device;
when the automatic controller receives a first confirmation instruction of the cup supply device and determines that the test cup is placed completely, the automatic controller controls the sample adding device to add a sample and a reagent into the test cup;
when the automatic controller receives a second confirmation instruction of the sample adding device and determines that the sample adding is finished, the automatic controller drives the resonance device to resonate;
generating a resonant force by the first resonant device and transmitting the resonant force to the insulation device;
the heat preservation device drives the test cup to resonate under the action of the resonance force, and the sample and the reagent in the test cup are uniformly mixed;
the resonance device driven by the automatic controller to resonate comprises:
sending a first voltage signal to a vibration motor arranged in a first resonance device through the automatic controller, driving the vibration motor to start rotating and generating resonance force in each direction through the first voltage signal, wherein the vibration motor is a high-speed eccentric wheel motor, and the frequency of the generated resonance force is 3KHz-5 KHz;
absorbing and filtering the resonance force in each direction through a linear slide rail arranged in the first resonance device, and obtaining the resonance force which is axially parallel to the linear slide rail;
the generating and transmitting of the resonant force to the temperature maintenance device by the first resonance device includes:
transmitting the resonance force axially parallel to the linear slide rail to the heat preservation device through a vibration base body arranged in the first resonance device;
the resonance device driven by the automatic controller to resonate comprises:
sending a second voltage signal to a piezoelectric ceramic oscillator arranged in the second resonance device through the automatic controller, driving the piezoelectric ceramic oscillator to start vibrating through the second voltage signal and generating a resonance force parallel to the central axis of the piezoelectric ceramic oscillator;
the method further comprises the following steps:
transmitting a resonance force parallel to a central axis of the piezoelectric ceramic oscillator to a vibration plate provided in the second resonance device by the piezoelectric ceramic oscillator;
the vibration plate drives a test cup in the cup groove structure of the vibration plate to resonate under the action of the resonance force, and the sample and the reagent in the test cup are uniformly mixed.
5. The method of claim 4, wherein controlling the cup feeding device to add the test cup to the incubator by the automatic controller comprises:
the automatic controller sends a cup moving instruction to the cup supplying device;
when the cup supplying device receives the cup moving instruction, the cup supplying device detects whether the uniformly mixed test cup is still placed in the heat preservation device;
if the uniformly mixed test cup is still placed in the heat preservation device, the uniformly mixed test cup is grabbed from the heat preservation device through the cup feeding device, and the uniformly mixed test cup is placed on the conveying device;
the cup supply device grabs an empty test cup from the test cup containing structure and places the empty test cup in the cup groove structure of the heat preservation device;
after the automatic controller receives the first confirmation instruction of the cup supply device and determines that the test cup is placed completely, the automatic controller controls the sample adding device to add the sample and the reagent into the test cup, and the method comprises the following steps:
the automatic controller sends a sample adding instruction to the sample adding device;
and when the sample adding device receives the sample adding instruction, adding a sample and a reagent into the empty test cup.
6. The method of claim 4, further comprising:
determining, by the automatic controller, a duration of time to drive the first resonating means to vibrate;
when the duration reaches a first preset duration, controlling to stop driving the vibration motor through the automatic controller;
under the elastic force action of the springs positioned at the two sides of the first resonance device, the test cup stops resonance and resets;
determining, by the automatic controller, a corresponding point in time to stop driving the first resonating means;
and after a second preset time period from the time point, the automatic controller continuously executes the step of controlling the cup supplying device to add the test cup into the heat preservation device.
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