CN104855386A - A bactericidal composition with metrafenone as the main component - Google Patents

Abstract

The invention discloses a sterilization composition, which comprises the effective components of metrafenone and boscalid in a mass ratio of 1: 100-100: 1. The composition has obvious synergism and high control efficiency, is suitable for controlling various diseases on fruits and vegetables, and is particularly suitable for controlling powdery mildew.

Description

A bactericidal composition with metrafenone as the main component

technical field

The invention relates to a bactericidal composition, in particular to a bactericidal composition with metrafenone as the main component and its application for preventing and treating crop diseases, belonging to the technical field of pesticides.

Background technique

Powdery mildew is a plant disease caused by fungi of the family Erysiphacesae. Mostly ectoparasitic, all or most of the mycelium is exposed on the surface of the leaves, stems, shoots, buds, flowers and fruits of the host plant, and produces a large number of mycelia, conidiophores and conidia A white powder visible to the naked eye.

Powdery mildew is widespread all over the world, especially in dicotyledonous plants. The main powdery mildews in China include cereal powdery mildew caused by Ery-siphe graminis; powdery mildew of melons, beans, hemp and other plants caused by Sphaerotheca fuliginea ; Cucumber powdery mildew caused by Uncinula necator; Powdery mildew of more than 80 kinds of trees such as mulberry, pear, persimmon, walnut, chestnut, litchi and papaya caused by Phyllactinia corylea; Powdery mildew of Rosa flowers caused by Sphaerotheca pannosa, etc.

Cucurbit powdery mildew is an important disease in melon production. When it first occurs, the leaves produce small yellow spots, and then expand and develop into round or oval lesions, with a white powdery mold layer on the surface, which are conidiophores and conidia of the fungus, and later the lesions turn yellowish brown and become the fungus closed capsule. The mildew spots are scattered separately in the early stage, and then combined to form a large mildew spot, which can even cover the whole leaf, seriously affecting photosynthesis, disturbing normal metabolism, causing premature aging, and loss of yield.

In the prevention and control of powdery mildew, fungicides such as benzimidazoles and triazoles are mainly relied on. Due to the long-term use of these agents, drug resistance has been produced to varying degrees, and the control effect has been reduced. In particular, triazoles can inhibit the growth of crops when used in large doses, and easily cause phytotoxicity.

Metrafenone, common name in English: metrafenone, is a new type of benzophenone fungicide with molecular formula C ₁₉ H ₂₁ BrO ₅ , which can effectively control powdery mildew of cereals, cucumbers and vegetables. However, at present, the production and use costs of metrafenone are relatively high, and long-term use alone will have a great risk of resistance.

Contents of the invention

The purpose of the present invention is to provide a fungicidal composition with obvious synergistic effect, good control effect on powdery mildew, low cost and low risk of resistance.

Another object of the present invention is to provide the application of the fungicidal composition.

Through a large number of indoor bioassays and field drug efficacy tests, the inventor unexpectedly found that when metrafenone and boscalid were mixed in a certain proportion, they had a significant synergistic effect on powdery mildew and other diseases.

Boscalid, a new type of nicotinamide fungicide, is mainly used to control powdery mildew, gray mold, various rots, brown rot and root rot. It belongs to the succinate coenzyme Q reductase inhibitor in the mitochondrial respiratory chain. It has a strong inhibitory ability to spore germination and has no cross-resistance with other fungicides.

Boscalid is a well-known fungicide in the art and can be obtained through various commercial sources.

In order to overcome the deficiencies in the prior art, the technical solution of the present invention is solved like this: a kind of bactericidal composition, contains active ingredient metrafenone and boscalid, the mass ratio of the two is 1:100～100:1, The preferred mass ratio is 1:30 to 30:1.

The active ingredients, auxiliary agents and fillers of the composition can be processed into any dosage forms allowed in agriculture, and the preferred dosage forms include emulsifiable concentrate, microemulsion, water emulsion, suspension concentrate, wettable powder and water dispersible granule.

In the bactericidal composition of the present invention, the cumulative mass percentage of active ingredients is 5% to 85%.

The dosage form of the above-mentioned bactericidal composition includes not only active ingredients, but also auxiliary agents and other substances that help to exert the drug effect. EC includes organic solvents, co-solvents and emulsifiers; microemulsions include organic solvents, emulsifiers and water; aqueous emulsions include organic solvents, dispersants, emulsifiers and water; suspending agents include water, dispersants, thickeners, antifreeze agents and wetting agents; wettable powders include wetting agents, dispersants and fillers; water-dispersible granules include dispersants, disintegrants, fillers and wetting agents. The above are various ingredients commonly used or allowed to be used in pesticide preparations, and there is no special limitation. The specific ingredients and dosage are determined through simple tests according to the formulation requirements. For example:

Organic solvents can be selected from isopropanol, xylene, N, N-dimethylformamide, cyclohexanone, toluene, dimethyl sulfoxide, methanol, ethanol, trimethylcycloketene, N-octylpyrrolidone, One or more of N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethyl acetate, vegetable oil.

The cosolvent can be selected from one or more of N-methylpyrrolidone, dimethylformamide, ethanol, propanol, and acetone.

Dispersants can choose sodium lignosulfonate, calcium lignosulfonate, pull-off powder, calcium dodecylbenzenesulfonate, polycarboxylate, calcium salt of alkylbenzenesulfonate, alkylphenol polyoxyethylene ether, One or more of fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ester, glycerin fatty acid ester polyoxyethylene ether.

The emulsifier can choose Nongru No. 600 (phenylphenol polyoxyethyl ether), Nongru No. 1601 (phenylethylphenol polyoxyethyl polypropylene ether), Nongru No. 500 (calcium alkylbenzene sulfonate) , OP series phosphate ester (nonylphenol polyoxyethylene ether phosphate ester), No. 600 phosphate ester (phenylphenol polyoxyethylene ether phosphate ester), styrene polyoxyethylene rice ammonium sulfate, alkyl biphenyl ether two Magnesium sulfonate salt, triethanolamine salt, Nongru No. 400 (benzyl xylenol polyoxyethyl ether), Nongru No. 700 (alkyl phenolic resin polyoxyethyl ether), Ningru No. 36 (phenylethyl ether) Phenol formaldehyde resin polyoxyethyl ether), Nongru No. 1600 (phenethylphenol polyoxyethyl polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxygen Vinyl ether), Nongru No. 33 (alkylaryl polyoxypropylene polyoxyethylene ether), Nongru No. 34 (alkylaryl polyoxyethylene polyoxypropylene ether), Span series (sorbitan monostearate One or more of Tween series (sorbitan fatty acid ester polyoxyethylene ether), AEO series (fatty alcohol polyoxyethylene ether).

The antifreeze can be selected from one or more of glycerol, urea, ethylene glycol, and propylene glycol.

Wetting agent can choose sodium methylnaphthalene sulfonate formaldehyde condensate, sodium lauryl sulfate, sodium alkylnaphthalene sulfonate, calcium alkylbenzene sulfonate, tea dry, saponin powder, silkworm excrement, sapindella powder, One or more of sodium lauryl sulfate, washing powder, and pull-off powder.

The disintegrant can be selected from one or more of ammonium sulfate, urea, bentonite, aluminum chloride, citric acid, succinic acid, and sodium bicarbonate.

The thickener can be selected from one or more of xanthan gum, hydroxymethylcellulose, methylcellulose, magnesium aluminum silicate, and polyvinyl alcohol.

The filler can be one or more of white carbon black, linseed clay, light calcium carbonate, talc, montmorillonite or attapulgite, pumice, broken brick, sepiolite or bentonite, and non-absorptive calcareous soil or sand kind.

The products described in the present invention can be provided in the form of finished preparations, that is, the substances in the composition have been mixed, and the ingredients of the composition can also be provided in single dose form, directly mixed in a barrel or tank before use, and then diluted to the required dosage. concentration.

The bactericidal composition of the present invention can be used to prevent and control cucumbers, watermelons, pumpkins, melons, zucchini, gourds, bitter gourds, towel gourds, wax gourds, cantaloupe, strawberries, peppers, cabbage, eggplants, potatoes, cucumbers and other crops caused by the fungus. , Ascomycetes, Basidiomycetes and other fungi caused plant diseases, especially for the control of powdery mildew. The compositions of the present invention can be applied by common methods, such as pouring, spraying, spraying, dusting, spreading or fuming, and the application rate varies with weather conditions or crop conditions.

Compared with the prior art, the present invention has the advantages of: 1. The synergistic effect of the composition is obvious, and the control effect is significantly improved compared with a single agent; 2. After the drug effect is improved, the amount of active ingredients in the field decreases, reducing production and The cost of use reduces pesticide residues and environmental pollution; 3. The composition is composed of active ingredients with different action mechanisms, and the number of action sites is increased, which is beneficial to overcome and delay the emergence of drug resistance of bacteria.

Detailed ways

Compounding the active ingredients of pesticides with different structural types is an effective way to solve the problems of cost and resistance in the process of single-dose application of pesticides. After the active ingredients of pesticides with different structural types are mixed, they usually exhibit three types of action, namely additive action, synergistic action and antagonistic action. However, the specific action cannot be predicted and can only be known through a large number of experiments. The formula with good compound and synergistic effects can improve the actual control effect, reduce the use of pesticides, and help delay the emergence of resistance. It is an important means of scientific control of pests and diseases.

The composition of the present invention has an obvious synergistic effect on powdery mildew bacteria, etc., rather than the simple addition of the effects of the two agents, which can be clearly seen from the results of the following indoor toxicity test.

Biological Assay Example: Indoor Toxicity Test of Compositions to Cucurbit Powdery Mildew Bacteria

The test object is cucumber powdery mildew (Sphaerotheca fuliginea).

Test method: refer to "People's Republic of China Agricultural Industry Standard NY/T 1156.11-2008". Two cucumber seedlings at the true leaf stage were selected in pots with the same growth potential, and 5 pots were selected for each treatment, and the numbers were reserved for future use. Spray with a Potter spray tower at a pressure of 50PSI, 5mL per pot. For the test treatments, see Table 1, Table 2, and Table 3, and 5 concentration gradients were set for each drug. After 24 hours, inoculate the conidia suspension of powdery mildew of cucumber with a concentration of 3×10 ⁵ spores/mL. The cucumber seedlings sprayed with the same amount of water were used as the blank control. After inoculation, the cucumber seedlings were cultured in the greenhouse. After 10 days, the disease index was investigated according to the incidence classification standard of cucumber powdery mildew, and the control effect was calculated.

Cucumber powdery mildew disease classification standard:

Grade 0: no disease spots on the leaves;

Grade 1: Lesion area accounts for less than 5% of the entire leaf area;

Grade 3: Lesion area accounts for 6% to 10% of the entire leaf area;

Grade 5: Lesion area accounts for 11% to 25% of the entire leaf area;

Grade 7: The lesion area accounts for 25% to 50% of the entire leaf area;

Grade 9: The lesion area accounts for more than 50% of the entire leaf area.

The expected potency (i.e. theoretical control effect, %) of the active ingredient mixture was determined using Colby's formula and compared to the observed potency:

Colby's formula: E=X+Y-X*Y/100

E: Expected efficacy of the composition when active ingredient A is used at concentration a and active ingredient B is used at concentration b in the composition.

X: The efficacy of active ingredient A when the dosage is a.

Y: The efficacy of active ingredient B when the dosage is b.

When the actual control effect observed exceeds the expected control effect, the mixture has a synergistic effect.

See Table 1 and Table 2 for toxicity test results.

The independent active compound of table 1 is to the pot test determination result of cucumber powdery mildew

Table 2 Pot test results of metrafenone and boscalid against cucumber powdery mildew

The test results showed that the combination of metrafenone and boscalid within a certain proportion had a synergistic effect on the control of cucumber powdery mildew.

In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention is illustrated with the following specific preparation examples, but the present invention is by no means limited to these examples. The following descriptions are only preferred embodiments of the present invention, and are only used to explain the present invention, and should not be construed as limiting the patent scope of the present invention. It should be noted that any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included within the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.

Cream Example

Mix the pesticide active ingredient, emulsifier, and part of the solvent in proportion, and mix and stir in the preparation kettle. After it is completely dissolved, slowly add the remaining solvent, and after fully stirring, a uniform oil phase is obtained to make an emulsifiable concentrate product.

Embodiment 1, 20.2% metrafenone boscalid emulsifiable concentrate

Metrafenone 20%, boscalid 0.2%, Nongru No. 500 2% (emulsifier), and xylene (solvent) were supplemented to 100%.

Example 2, 15.5% Metrafenone Boscalid EC

Metrafenone 15%, boscalid 0.5%, Nongru No. 500 3% (emulsifier), and xylene (solvent) were supplemented to 100%.

Microemulsion, water emulsion embodiment

According to the distribution ratio of each component, the active component is dissolved in a solvent and a co-solvent, a surfactant is added, and the oil phase is obtained by mixing evenly. The water-soluble component and water are mixed to obtain a water phase, and then the oil phase is added to the water phase or the water phase is added to the oil phase, and stirred while adding to obtain a microemulsion or a water emulsion.

Embodiment 3, 5% metrafenone boscalid microemulsion

Metrafenone 2.5%, Boscalid 2.5%, Toluene 8% (solvent), Nongru No. 500 8% (emulsifier), Nongru No. 600 5% (emulsifier), Nongru No. 1600 5% (emulsifier) agent), sodium lauryl sulfate 2% (humectant), ethylene glycol 5% (antifreeze), water replenished to 100%

Embodiment 4, 15% metrafenone boscalid water emulsion

Metrafenone 5%, Boscalid 10%, DMF (solvent) 5%, Cyclohexanol (solvent) 10%, Glycerin (antifreeze) 5%, Phenylphenol polyoxyethylene ether (emulsifier) 10% %, calcium dodecylbenzenesulfonate (wetting agent) 5%, water added to 100%

Example of Suspending Concentrate

Mix the active ingredient, dispersant, defoamer, thickener and water according to the proportion of the formula, and get a semi-finished product after sand milling or high-speed shearing. After analysis, add water, mix evenly and filter to get the suspending agent.

Example 5, 31% Metrafenone Boscalid Suspension Concentrate

Metrafenone 1%, Boscalid 30%, Nonylphenol Polyoxyethylene Ether 8% (Emulsifier), Propylene Glycol 2% (Solubilizer), Xanthan Gum 1% (Thickener), Silicone 2% (defoamer), water added to 100%

Example 6, 30.3% Metrafenone Boscalid Suspension Concentrate

Metrafenone 0.3%, Boscalid 30%, Nonylphenol Polyoxyethylene Ether 8% (Emulsifier), Propylene Glycol 2% (Solubilizer), Xanthan Gum 1% (Thickener), Silicone 2% (defoamer), water added to 100%

Examples of water dispersible granules

The pesticide active components, auxiliary agents and fillers are mixed in proportion, pulverized by air flow, and then 15-20% of water is added, kneaded, granulated, dried and sieved to obtain a water-dispersible granule product. Main equipment: mixer, jet mill, kneader, extrusion granulator, drying equipment (drying room or fluidized bed).

Example 7, 85% metrafenone boscalid water dispersible granules

Metrafenone 50%, Boscalid 35%, Sodium dodecylbenzene sulfonate 4% (wetting agent), Sodium lignosulfonate 10% (dispersant), Ammonium sulfate 3% (disintegrant), Kaolin (filler) to 100%

Examples of wettable powder

The pesticide active components, auxiliary agents and fillers are mixed in proportion, air-jet pulverized and then mixed to obtain wettable powder. Main equipment: mixer, jet mill.

Example 8, 50% metrafenone boscalid wettable powder

Metrafenone 15%, Boscalid 35%, Sodium Lauryl Sulfate 4% (wetting agent), Alkane Sulfonate Sodium Salt 7% (dispersant), White Carbon Black 8% (filler), Kaolin (filler) ) to 100%

Example 9, 35% metrafenone boscalid wettable powder

Metrafenone 25%, Boscalid 10%, Sodium Lauryl Sulfate 4% (wetting agent), Alkane Sulfonate Sodium Salt 7% (dispersant), White Carbon Black 8% (filler), Kaolin (filler) ) to 100%

Field application example: Field efficacy test of combination of metrafenone and boscalid against cucumber powdery mildew

The test site is Sanya, Hainan. See Table 3 for the test agents and their dosages. Each treatment was repeated in 4 plots, each with an area of 20 square meters. The first spraying was carried out at the early stage of disease occurrence, sprayed evenly, and sprayed until the leaves dripped, and the clean water treatment was used as the blank control. Apply the second medicine after 7 days. The incidence of cucumber powdery mildew was investigated before the first spraying and 10 days after the second spraying. Five-point sampling was adopted in each plot, and two plants were surveyed at each point, and all leaves were surveyed for each plant, and the percentage of diseased spot area to the entire leaf area was used to classify and record. The disease index and control effect of each treatment were calculated.

Grading standards:

Grade 0: no lesions;

Grade 1: Lesion area accounts for less than 5% of the entire leaf area;

Grade 3: The lesion area accounts for 6%-15% of the entire leaf area;

Grade 5: The lesion area accounts for 16%-25% of the entire leaf area;

Grade 7: The lesion area accounts for 26%-50% of the entire leaf area;

Grade 9: The lesion area accounts for more than 50% of the entire leaf area.

Drug efficacy calculation method:

As can be seen from Table 3: the control effects of metrafenone and boscalid single agents on cucumber powdery mildew were 75.5% and 76.0% respectively, while the fungicidal compositions of the examples of the present invention had significantly improved control effects, with the lowest The control effect was 84.2%, and the highest reached 89.2%. The results of field experiments fully show that the combination of metrafenone and boscalid has a significant synergistic effect on cucumber powdery mildew, and the control effect is greatly improved when the dosage of active ingredients per mu is reduced compared with that of a single agent. Therefore, the composition of the present invention has the beneficial effects of reducing costs, delaying resistance, reducing application times and pesticide residues.

Table 3 Field test results of combination of metrafenone and boscalid against cucumber powdery mildew

Claims (5)

1. A bactericidal composition, characterized in that: the active ingredients are metrafenone and boscalid, and the mass ratio of the two is 1:100 to 100:1. 2. The fungicidal composition according to claim 1, characterized in that the mass ratio of metrafenone to boscalid is 1:30-30:1. 3. The fungicidal composition according to claim 1, characterized in that: the cumulative mass percentage of active ingredients in the composition is 5%-85%. 4. The bactericidal composition according to any one of claims 1 to 3, characterized in that: the dosage form is emulsifiable concentrate, microemulsion, water emulsion, suspension concentrate, wettable powder or water dispersible granule. 5. The fungicidal composition of claim 1 is used in the prevention and treatment of cucurbit powdery mildew.