CN103642683A - Polymerase chain reaction (PCR) instrument capable of performing multiple PCR experiments - Google Patents
Polymerase chain reaction (PCR) instrument capable of performing multiple PCR experiments Download PDFInfo
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- CN103642683A CN103642683A CN201310706670.0A CN201310706670A CN103642683A CN 103642683 A CN103642683 A CN 103642683A CN 201310706670 A CN201310706670 A CN 201310706670A CN 103642683 A CN103642683 A CN 103642683A
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- pcr
- sample pool
- pcr instrument
- instrument
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- 238000002474 experimental method Methods 0.000 title abstract description 12
- 238000003752 polymerase chain reaction Methods 0.000 title abstract 11
- 230000013011 mating Effects 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000005265 energy consumption Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 28
- 238000005516 engineering process Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910001120 nichrome Inorganic materials 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a polymerase chain reaction (PCR) instrument capable of performing multiple PCR experiments. The PCR instrument comprises an enclosure, and a display screen and a control panel which are arranged on the enclosure, wherein a sample cell and a sample cell cover are arranged on the enclosure; grooves used for dividing the sample cell are formed in the sample cell; clapboards matched with the grooves are arranged on the sample cell cover; heating wires in the enclosure are respectively arranged in all compartments in the sample cell. The PCR instrument has the obvious advantages that multiple PCR experiments can be performed on the same machine according to the PCR instrument, the time is saved, the experimental period is shortened, and the space lab is saved. Moreover, with the multiple heating wires, the temperatures in different compartments are accurately controlled, the energy is saved, the energy consumption is reduced, and the PCR instrument is convenient to use.
Description
Technical field
The present invention relates to a kind of PCR instrument, particularly a kind of PCR instrument that can simultaneously carry out multinomial PCR.
Background technology
pCR is the important technology of life science, medical field, and round pcr is used for target dna to increase in a large number, thereby further carries out follow-up research work.PCR instrument is the key instrument that carries out PCR, and the appearance of PCR instrument makes this technology realize automatization, makes the technology application of PCR more extensive, for example
In diagnosing hereditary illness, detect clinical samples in pathogenic agent nucleic acid, legal medical expert's sample made to genetics identify, and the aspects such as sudden change situation in analysis activated oncogene are all widely used, its cardinal principle is to heat up to make DNA sex change, then cooling makes DNA annealing, extends, and needs to control accurately temperature in this process.
Current PCR instrument is single instrument, can only carry out a PCR experiment, yet in common experimentation, not have so much sample and can pile 96 orifice plates, therefore causes waste, also causes unnecessary using to consume and waste.
Summary of the invention
For solving the deficiencies in the prior art, the object of the present invention is to provide a kind of PCR instrument that can simultaneously carry out multinomial PCR, save the energy, easy to use.
In order to realize above-mentioned target, the present invention adopts following technical scheme:
A kind of PCR instrument that simultaneously carries out multinomial PCR, comprise casing, be located at display screen and control panel on casing, on described casing, be also provided with sample pool and sample pool lid, it is characterized in that, be provided with the groove for sample pool is separated in described sample pool, described sample pool covers and is provided with the dividing plate mating with groove, for dividing plate described in when closing sample pool lid, can just in time insert groove, in described casing, heater strip is separately positioned in sample pool in each compartment.
A PCR instrument that simultaneously carries out multinomial PCR, is characterized in that, described groove number is more than 2, and the compartment number that sample pool is evenly divided into is more than 4.
A PCR instrument that simultaneously carries out multinomial PCR, is characterized in that, described groove is 4, and sample pool is evenly divided into 8 compartments.
A PCR instrument that simultaneously carries out multinomial PCR, is characterized in that, described dividing plate outside surface is provided with tightness system.
A PCR instrument that simultaneously carries out multinomial PCR, is characterized in that, described tightness system is diaphragm seal.
Usefulness of the present invention is: 1. the PCR instrument that simultaneously carries out multinomial PCR of the present invention can carry out multinomial PCR experiment on a machine simultaneously, saves time, and shortens experimental period, saves lab space; 2. by a plurality of nichrome wire are set, reach the temperature of the different compartments of accurate control, save energy, reduces power consumption, easy to use.
Accompanying drawing explanation
Fig. 1 is the PCR instrument schematic diagram that simultaneously carries out multinomial PCR of the present invention;
Fig. 2 is PCR instrument sample pool lid schematic internal view of simultaneously carrying out multinomial PCR of the present invention.
The implication of Reference numeral in figure: 1. casing, 2. sample pool lid, 3. display screen, 4. control panel, 5. sample pool, 6. groove, 7. dividing plate.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is done to concrete introduction.
In conjunction with Fig. 1, the PCR instrument that simultaneously carries out multinomial PCR of the present invention, comprises casing 1, and is located at display screen 3 and control panel 4 on casing 1, described control panel 4 is for setting various parameters according to user, and display screen 3 is for display parameter and temperature trend; On described casing, be also provided with sample pool 5 and sample pool lid 2, in described sample pool 5, be provided with 2 of the intersection groove 6 for sample pool 5 is separated, groove 6 is evenly divided into 4 compartments by sample pool 5, described sample pool lid 2 is provided with in the dividing plate 7(of groove 6 couplings as shown in Figure 2), dividing plate 7 outside surfaces are provided with diaphragm seal, for covering 2 o'clock at the sample pool that closes, dividing plate 7 reaches good sealing effectiveness while inserting groove 6; The interior heater strip (not shown) of described casing 1 is separately positioned in sample pool 5 in each compartment, for different compartments are heated, required Heating temperature because of requirement of experiment different in different compartments different.
The PCR instrument that simultaneously carries out multinomial PCR of the present invention, except said structure, its difference is, described groove 6 is 4, and sample pool 5 is evenly divided into 8 compartments.
The PCR instrument that simultaneously carries out multinomial PCR of the present invention, except said structure, its difference is, described groove 6 is 3, and sample pool 5 is evenly divided into 6 compartments.
Using method of simultaneously carrying out the PCR instrument of multinomial PCR of the present invention is: 1. each compartment of different laboratory samples 96 orifice plates being put into sample pool 5, the sample pool that closes lid 2, now the dividing plate 7 on sample pool lid 2 inserts in the groove 6 in sample pool lid 2, uses so that each compartment seals, insulation; 2. set temperature required and time of each compartment different experiments, start experiment, in experimentation, temperature collecting device has gathered the parameter of input on control panel 4, and information is sent to treater, treated device is sent to the heater strip in each compartment again, and each heater strip is heated each compartment, thereby reaches the temperature control of different experiments in different compartments.
Feature of simultaneously carrying out the PCR instrument of multinomial PCR of the present invention has:
Break laboratory and wait for that for doing PCR experiment machine, instrument take larger space isotype, the present invention makes full use of space can realize a plurality of experiments on a machine, easy to use, does not increase complex operations, saves the energy simultaneously, has increased scientific research efficiency.
The PCR instrument that simultaneously carries out multinomial PCR of the present invention puts into production, and tries out, respond well, is applicable to various laboratories, hospital carries out various PCR experiments.
More than show and described ultimate principle of the present invention, principal character and advantage.The technician of the industry should understand, and above-described embodiment does not limit the present invention in any form, and all employings are equal to replaces or technical scheme that the mode of equivalent transformation obtains, all drops in protection scope of the present invention.
Claims (5)
1. a PCR instrument that simultaneously carries out multinomial PCR, comprise casing, be located at display screen and control panel on casing, on described casing, be also provided with sample pool and sample pool lid, it is characterized in that, be provided with the groove for sample pool is separated in described sample pool, described sample pool covers and is provided with the dividing plate mating with groove, for dividing plate described in when closing sample pool lid, can just in time insert groove, in described casing, heater strip is separately positioned in sample pool in each compartment.
2. the PCR instrument that simultaneously carries out multinomial PCR according to claim 1, is characterized in that, described groove number is more than 2, and the compartment number that sample pool is evenly divided into is more than 4.
3. the PCR instrument that simultaneously carries out multinomial PCR according to claim 2, is characterized in that, described groove is 4, and sample pool is evenly divided into 8 compartments.
4. the PCR instrument that simultaneously carries out multinomial PCR according to claim 1, is characterized in that, described dividing plate outside surface is provided with tightness system.
5. the PCR instrument that simultaneously carries out multinomial PCR according to claim 4, is characterized in that, described tightness system is diaphragm seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310706670.0A CN103642683A (en) | 2013-12-19 | 2013-12-19 | Polymerase chain reaction (PCR) instrument capable of performing multiple PCR experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310706670.0A CN103642683A (en) | 2013-12-19 | 2013-12-19 | Polymerase chain reaction (PCR) instrument capable of performing multiple PCR experiments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103642683A true CN103642683A (en) | 2014-03-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310706670.0A Pending CN103642683A (en) | 2013-12-19 | 2013-12-19 | Polymerase chain reaction (PCR) instrument capable of performing multiple PCR experiments |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103642683A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105647795A (en) * | 2016-02-25 | 2016-06-08 | 李亮亮 | Novel microbial enzyme rapid detection device based on PCR (polymerase chain reaction) technique |
| CN116087103A (en) * | 2023-01-13 | 2023-05-09 | 中国科学院空间应用工程与技术中心 | Sample stage and sample analysis system applicable to infrared and Raman spectrum analysis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2543963Y (en) * | 2002-02-07 | 2003-04-09 | 张家林 | Temp-controller for two-D grad gene amplificator |
| US20070110634A1 (en) * | 1999-10-01 | 2007-05-17 | Applera Corporation | Device for the carrying out of chemical or biological reactions |
| CN101735949A (en) * | 2008-11-05 | 2010-06-16 | 广达电脑股份有限公司 | variable temperature device |
| CN202830042U (en) * | 2012-09-25 | 2013-03-27 | 黑龙江中医药大学 | PCR (polymerase chain reaction) instrument |
-
2013
- 2013-12-19 CN CN201310706670.0A patent/CN103642683A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070110634A1 (en) * | 1999-10-01 | 2007-05-17 | Applera Corporation | Device for the carrying out of chemical or biological reactions |
| CN2543963Y (en) * | 2002-02-07 | 2003-04-09 | 张家林 | Temp-controller for two-D grad gene amplificator |
| CN101735949A (en) * | 2008-11-05 | 2010-06-16 | 广达电脑股份有限公司 | variable temperature device |
| CN202830042U (en) * | 2012-09-25 | 2013-03-27 | 黑龙江中医药大学 | PCR (polymerase chain reaction) instrument |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105647795A (en) * | 2016-02-25 | 2016-06-08 | 李亮亮 | Novel microbial enzyme rapid detection device based on PCR (polymerase chain reaction) technique |
| CN116087103A (en) * | 2023-01-13 | 2023-05-09 | 中国科学院空间应用工程与技术中心 | Sample stage and sample analysis system applicable to infrared and Raman spectrum analysis |
| CN116087103B (en) * | 2023-01-13 | 2023-11-07 | 中国科学院空间应用工程与技术中心 | Sample stage and sample analysis system applicable to infrared and Raman spectrum analysis |
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Application publication date: 20140319 |