CL2011000665A1 - Molecula de acido nucleico que codifica subunidad grande de la enzima de sintasa de brassica; vector de expresion que la comprende; metodo de control de malezas. - Google Patents
Molecula de acido nucleico que codifica subunidad grande de la enzima de sintasa de brassica; vector de expresion que la comprende; metodo de control de malezas.Info
- Publication number
- CL2011000665A1 CL2011000665A1 CL2011000665A CL2011000665A CL2011000665A1 CL 2011000665 A1 CL2011000665 A1 CL 2011000665A1 CL 2011000665 A CL2011000665 A CL 2011000665A CL 2011000665 A CL2011000665 A CL 2011000665A CL 2011000665 A1 CL2011000665 A1 CL 2011000665A1
- Authority
- CL
- Chile
- Prior art keywords
- nucleic acid
- acid molecule
- ahasl
- brassica
- protein
- Prior art date
Links
- 102000039446 nucleic acids Human genes 0.000 title abstract 6
- 108020004707 nucleic acids Proteins 0.000 title abstract 6
- 150000007523 nucleic acids Chemical class 0.000 title abstract 6
- 241000219198 Brassica Species 0.000 title abstract 4
- 241000196324 Embryophyta Species 0.000 title abstract 4
- 235000011331 Brassica Nutrition 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- 239000013604 expression vector Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 abstract 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Chemical group OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 abstract 1
- 240000002791 Brassica napus Species 0.000 abstract 1
- 235000011293 Brassica napus Nutrition 0.000 abstract 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical group C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 abstract 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
- 239000000654 additive Substances 0.000 abstract 1
- 230000000996 additive effect Effects 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 230000002363 herbicidal effect Effects 0.000 abstract 1
- 239000004009 herbicide Substances 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8278—Sulfonylurea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Catching Or Destruction (AREA)
Abstract
MOLÉCULA DE ÁCIDO NUCLEICO RECOMBINANTE/MUTAGENIZADA, QUE CODIFICA UNA PROTEÍNA DE SUBUNIDAD MAYOR DE ACETOHIDROXIÁCIDO SINTASA 111 (AHASL) DE BRASSICA NAPUS QUE COMPRENDE UNA SUSTITUCIÓN DE ALANINA POR TREONINA EN LA POSICIÓN A122 DE LA 5EQ ID NO:23 (A122T) Y UNA SUSTITUCIÓN DE SERINA POR ASPARAGINA EN LA POSICIÓN 5653 DE LA 5EQ ID NO:23 (5653N), DONDE DICHA AHASL ES RESISTENTE A LA INHIBICIÓN POR UN HERBICIDA DE IMIDAZOLINONA, Y DONDE LA PROTEÍNA AHASL DEMUESTRA UN NIVEL SINÉRGICO DE RESISTENCIA A LA INHIBICIÓN POR HERBICIDA DE IMIDAZOLINONA COMPARADO CON LOS<br /> NIVELES ADITIVOS DE LA PROTEÍNA DE AHASL 111 DE BRASSLCA NAPUS QUE TIENE UNA ÚNICA MUTACIÓN EN A122T O EN S653N, RESPECTIVAMENTE. VECTOR DE EXPRESIÓN QUE COMPRENDE A DICHA MOLÉCULA DE ÁCIDO NUCLEICO. MÉTODO DE CONTROL DE MALEZAS EN LA VECINDAD DE UNA PLANTA DE BRASSICA QUE COMPRENDE EN SU GENOMA A LA MOLÉCULA DE ÁCIDO NUCLEICO DE AHASL MUTAGENIZADA. MÉTODO DE REPRODUCCIÓN DE DICHA PLANTA DE BRASSICA. CÉLULA DE PLANTA, QUE COMPRENDE A DICHA MOLÉCULA DE ÁCIDO NUCLEICO, O DE CUALQUIERA DE LAS LINEAS DE PLANTAS BNCL131A1, BNCL140B3, BNCL140C7, O PM1PM2/BNCL131AL,<br /> O QUE COMPRENDE LAS CARACTERÍSTICAS DE RESISTENCIA A HERBICIDAS, DE DICHAS LÍNEAS DE PLANTAS. MÉTODO PARA CONTROLAR MALEZAS, QUE UTILIZA A DICHA PLANTA DE BRASSICA. MÉTODO PARA IDENTIFICAR UNA MOLÉCULA DE ÁCIDO NUCLEICO AHAS MUTAGENIZADA EN UNA MUESTRA BIOLOGICA.<br />
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10054108P | 2008-09-26 | 2008-09-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2011000665A1 true CL2011000665A1 (es) | 2011-08-12 |
Family
ID=41351460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2011000665A CL2011000665A1 (es) | 2008-09-26 | 2011-03-25 | Molecula de acido nucleico que codifica subunidad grande de la enzima de sintasa de brassica; vector de expresion que la comprende; metodo de control de malezas. |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US20120060243A1 (es) |
| EP (3) | EP3441470A1 (es) |
| JP (2) | JP6133011B2 (es) |
| KR (1) | KR20110080154A (es) |
| CN (2) | CN102216453B (es) |
| AP (1) | AP2011005671A0 (es) |
| AR (1) | AR073402A1 (es) |
| AU (2) | AU2009296625B2 (es) |
| BR (1) | BRPI0919380A2 (es) |
| CA (1) | CA2737939C (es) |
| CL (1) | CL2011000665A1 (es) |
| EA (2) | EA201691309A1 (es) |
| GE (1) | GEP20146179B (es) |
| MX (1) | MX2011003266A (es) |
| NZ (2) | NZ607411A (es) |
| PL (2) | PL2342331T3 (es) |
| UA (1) | UA115019C2 (es) |
| UY (1) | UY32144A (es) |
| WO (3) | WO2010036771A2 (es) |
| ZA (1) | ZA201102076B (es) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2636771C (en) | 2006-01-12 | 2016-05-24 | Greg F.W. Gocal | Epsps mutants |
| EA030583B8 (ru) * | 2010-03-17 | 2018-11-30 | Басф Агрокемикэл Продактс Б.Ф. | Гербицидостойкие растения |
| RS57062B1 (sr) * | 2010-03-17 | 2018-06-29 | Basf Agrochemical Products Bv | Biljke tolerantne na herbicide |
| UA112969C2 (uk) * | 2010-08-03 | 2016-11-25 | Сібас Юс Ллс | Рослина, стійка до одного або більше ррх-інгібуючих гербіцидів, яка містить мутантний ген протопорфіриноген ix оксидази (ррх) |
| UA113721C2 (uk) | 2010-10-15 | 2017-03-10 | Баєр Інтеллекчуел Проперті Гмбх | Толерантний до інгібуючого als гербіциду мутант буряку звичайного |
| UA117447C2 (uk) | 2011-05-04 | 2018-08-10 | Байєр Інтеллектуал Проперті Гмбх | Застосування гербіцидів інгібіторів als для боротьби з небажаною рослинністю на площі вирощування brassica |
| EP2704555A1 (en) | 2011-05-04 | 2014-03-12 | Bayer Intellectual Property GmbH | Als inhibitor herbicide tolerant b. napus mutants |
| BR102012019434B1 (pt) * | 2011-07-26 | 2021-11-09 | Dow Agrosciences Llc | Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1 |
| RU2014126423A (ru) * | 2011-11-29 | 2016-01-27 | Агридженетикс, Инк. | Высокопроизводительный анализ однонуклеотидных полиморфизмов |
| IN2014CN04928A (es) * | 2011-12-05 | 2015-09-18 | Basf Agrochemical Products Bv | |
| US20150089682A1 (en) | 2012-02-29 | 2015-03-26 | Bayer Cropscience Nv | ALS Inhibitor Herbicide Tolerant B. Napus Mutants |
| MX366944B (es) * | 2012-04-05 | 2019-07-31 | Advanta Int Bv | Plantas de sorgo que tienen un polinucleotido mutado que codifica una subunidad mayor de la proteina sintasa acetohidroxiacida mutada y tiene resistencia incrementada a herbicidas. |
| BR112014031260A2 (pt) | 2012-06-15 | 2019-08-20 | Du Pont | métodos e composições que envolvem variantes de als com preferência de substrato nativo |
| US9957515B2 (en) | 2013-03-15 | 2018-05-01 | Cibus Us Llc | Methods and compositions for targeted gene modification |
| WO2015004242A1 (en) | 2013-07-12 | 2015-01-15 | Bayer Cropscience Nv | Als inhibitor herbicide tolerant mutant plants |
| KR102450868B1 (ko) * | 2014-03-14 | 2022-10-06 | 시버스 유에스 엘엘씨 | 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물 |
| CN104126496B (zh) * | 2014-07-03 | 2016-08-24 | 华中农业大学 | 利用抗除草剂基因的油菜化学杀雄制种方法 |
| CN104531598B (zh) * | 2015-01-08 | 2018-01-16 | 齐鲁工业大学 | 一种重组链霉菌、其构建方法及提高抗生素产量的方法 |
| WO2016130247A1 (en) * | 2015-01-10 | 2016-08-18 | Cibus Us Llc | Mutated acetohydroxyacid synthase genes in euphorbiaceae and plant material comprising such genes |
| JP2017118878A (ja) * | 2017-01-16 | 2017-07-06 | アドヴァンタ・ホールディングス・ベスローテン・フェンノートシャップ | 突然変異アセトヒドロキシ酸合成酵素タンパク質のラージサブユニットをコードする突然変異ポリヌクレオチドを有し、除草剤耐性が増大したソルガム植物 |
| CN110545659A (zh) * | 2017-01-31 | 2019-12-06 | 赖斯泰克有限公司 | 多个突变对提高水稻中的除草剂抗性/耐受性的作用 |
| US11345924B2 (en) * | 2017-05-11 | 2022-05-31 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | Creation of herbicide resistant gene and use thereof |
| JP2019141045A (ja) * | 2019-03-04 | 2019-08-29 | アドヴァンタ・ホールディングス・ベスローテン・フェンノートシャップ | 突然変異アセトヒドロキシ酸合成酵素タンパク質のラージサブユニットをコードする突然変異ポリヌクレオチドを有し、除草剤耐性が増大したソルガム植物 |
| KR102147381B1 (ko) * | 2019-11-22 | 2020-08-24 | 씨제이제일제당 주식회사 | 아세토하이드록시산 신타제 신규 변이체 및 이를 포함하는 미생물 |
| CN113584218A (zh) * | 2021-09-09 | 2021-11-02 | 重庆大学 | 与谷子抗咪唑乙烟酸除草剂基因紧密连锁的分子标记sv9和sv13及其应用 |
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