CA2278383A1

CA2278383A1 - Stimulators for living organisms

Info

Publication number: CA2278383A1
Application number: CA002278383A
Authority: CA
Inventors: Strashko Volodymyr
Original assignee: Individual
Current assignee: Individual
Priority date: 1999-07-21
Filing date: 1999-07-21
Publication date: 2001-01-21

Abstract

The method of treatment of the green plants with the chelates of bioactive metals, iron, manganese, magnesium, zinc, copper and cobalt with the ethylenediamine-N,N'-dicarbonic acid and oxyacids, like citric, tartaric and ascorbic acids, as the second ligands, in water solution with the concentration being in the range of 0.01-0.25 mass %
of the each metal ions, to increase the harvesting crops of seeds, fruits, green mass and amount and quality of flowers for oil-productive plants, horticultural plants, fruits and vegetable cultures, citrus's in open ground and in greenhouses, which are naturally utilized, decomposing to the fragments of essential aminoacids and their complexes with the metal ions, carbon dioxide, ammonia and traces common organic acids, like acetic acid, glutamic acid, etc., under sunlight influence and in metabolic cycle of plants. The formulations varying for application for different types of plants and give an averaged increasing of crops up to 20-50 %%, depends on the type of culture. The method of treatment of fur animals, pets and poultry with the aforenamed compounds, that cures the white-puffs, stresses, increases the hemoglobin content in blood and increases the growth rate and quality of fur. Complexes are non-toxic, environmental friendly. and working as biostimulators and micro-fertilizers.

Description

STIMULATORS FOR LIVING ORGANISMS
RELATED APPLICATIONS
This application relates to my former USSR patent No. 1096919 "S", issued February 8, 1984, and USSR patent No. 1614434"S", issued August 15,1990.
FIELDS OF INVENTION
This invention relates to the compositions and application of the chelate complexes of the bioactive metals with ethylenediamine-N,N'dicarbonic acid, oxyacids, like tartaric acid, citric acid, ascorbic acid and boric acid.
More particularly, the invention related to the improved method of preparing soluble complexes and compositions of the ethylenediamine-N, N'-disuccinate (edds), of metal ions of iron, zinc, manganese, magnesium, cobalt, copper and mixed complexes of aforenamed ions with edds and oxyacids of tartaric, citric and boric acids;
the application of these complexes as environmental friendly microfertilizes and biostimulators of growth, seeds, flowers and fruits production, nectar productivity of green plants, curing agents for animals and poultry.
Further, the invention relates to the application of the compositions for the green plants and consists of the dilute solution of formulations treatment directly to the foliage of plants in open ground or in greenhouses once a fortnight or once a month, depending of the specific type of plants and including stimulating effects of complexes and their fragments on the processes of growth, nectar productivity, seeds/ fruits formation. These goals achieved by direct penetration of the complexes and their fragments through the foliage and promoting the chlorophyll formation and intensification of vital activity of the whole plants, as result of treatments.
The application of such types of complexes to the plants leads to the growing of the crops harvesting from 20 till 50 % and do not pollute the plant's tissues, fruits, seeds and environment with any toxic compounds.
Further, the invention deals with the application of iron, zinc, manganese and magnesium compositions for curing the 1'' and 2'~ phases of chlorosis in grapes, fruit trees, flax, cucumbers and tomatoes in full and partly the 3'~ phase of chlorosis.
Still further, the invention relates to the application of iron, cobalt, copper and magnesium complexes aforesaid ligands in veterinary and for prophylaxis of fur animals, for pets and for poultry farming to cure such types of diseases, as white-puffs, stresses, to improve the quality of fur of animals, to increase the vital activity of animals and poultry.

2 DESCRIPTION OF THE PRIOR ART
The shortage of the bioactive metals in the green plants leads to the depressing of the growth and finally to the decreasing of the crops harvesting of the main agricultural products. To overcome this effect, the greater amount of growth stimulators, microelements and macrofertilizes are used in all branches of agriculture.
Basically, three approaches are used now to reach the positive erects:
a. The increasing of the amount of conventional fertilizes and / or preparation these fertilizes in the more appropriate forms;
b. The application of microelements with the macrofertilizes in the forms of inorganic salts, some complex compounds of metals in different combinations;
c. The application of organic stimulators of plant growth, for example indole-derivatives, to increase the rates of plant's growth and to increase the rates of roots, fruits formations.
All of them have some negative consequences. The increasing of the water-soluble plant nutrients in soils may cause phytotoxicity to the plants, increasing the cost of agricultural production and leads to the pollution of the soils and waters with the basic phosphorus, nitrogen, potassium compounds. That erecting to the main nutrition chains of the nature and makes dramatic changes in the environment. For the last decades a lot of improvements were proposed to overcome the negative effects of overloading of basic fertilizes to the soils: preparing the granulated nutrients, coated nutrients, normalizing the amounts of fertilizes to the specific areas, using the microelements with the basic nutrients.
These microelements are the sufficient component of ferments producing by plants and play the key roles in vital processes. Application of the bioactive metal ions provides the increasing of growth and decreasing of macro-nutrients consumption, that in turn depletes the contamination and lowing the cost of agriproducts. This trend is the most perspective for the agriculture, but still demands the further improvements.
The application of the organic stimulators may effected to the genetic structure and mechanism of natural material and thus can lead to long-term negative effects for the customers of agricultural products.
Additionally, it may be taken in account the fourth way - genetic engineering of new types of plants, but it is out of our consideration.
Researchers for a long time have attempted to develop the most effective types of bioactive metals compositions. These compound should be soluble in the large scale of pH, must be effectively utilized by the plants and they should be accessible and at competitive prices.
One of the most appropriate compounds are so called complexonates- chelate complexes of metals. Well known ethylenediamine-N, N'-tetraacetates of metals are widely used in agriculture as microelements suppliers.
These chelates of bioactive metals are rather acceptable, as they are soluble in the pH
range of 3 till 10, they can be produced easily and gave good results in practice.

Unfortunately, these compounds do not decompose in full under natural conditions and have a little stimulating effect, as the complexes are very firm.
Complexes of biometals with the aminoacids and some additives are too expensive and not demonstrated the perfect results.
Many of these shortcomings in the prior art were overcome in my former USSR
patent No. 1096919 "S" , issued February 8, 1984, which discloses the synthesis and application of the Fe-edds, Zn-edds and their different compositions as the effective microfertilizes and biostimulators of seed formation and nectar- production in Lucerne.
My former USSR patent No. 1614434"S" issued August 15,1990, also deals with the stimulating effects of growth, seeds formation and nectar-productivity of buckwheat provided by iron- containing edds, tartaric acid complexes.
The methods described in my aforesaid USSR patents apply the particular complexes to some particular plants. The present invention now provides the whole lot of edds-oxyacids- bioactive metals complexes, their preparation and application for the plants as biostimulators and microfertiLizes.
SUMMARY OF THE INVENTION
Accordingly, this invention seeks to provide a new and improved method of treatment of plants to stimulate the growth, seeds and green masses production of the honey planted nectar-productive ( polygonum, clover), feeding ( Lucerne, rape, sweetclover, cockshead sainfoin) and technical (flax, hop) cultures.
Further, this invention seeks to provide the improved treatment of floricuLtural plants stimulating the increasing of the quantity and quality of flowers, providing the thickening of stems, curing completely the first and second phases of ferruginous and magnesium chlorosis.
Still further, thisinvention seeks to provide the improved method of treatment of horticultural and viticuLtural plants, stimulating the growth of quantity and quality of fruits and berries, curing the first, second and, partly, the third phases of chlorosis.
Still further, this invention seeks to provide the increasing of growth and amount of fruits in vegetable's production in open ground and in greenhouses, by actively stimulating the vitality, chlorophyll content in leaves, curing completely the ferruginous and magnesium chlorosis.
Still further, this invention seeks to provide the curing of the white-puffs in fur animals and pets, increasing the hemoglobin content in blood and increasing the growth rate and quality of fur for animals, increasing the rate of growth and curing the stresses for poultry.
The present invention provides an improved and simplified method of preparation of the effective blends and formulations of bioactive chelate compositions directly from the tetrasodium/potassium salts of ethylenediamine-N,N'-disucarbonic acid, oxyacids, like tartaric, citric acids and boric acid and related metal salts.
The invention in one broad aspect provide a method of the treatment of green plants to stimulate the processes of growth and productivity of the plants and to stimulate vital activity of animals, comprising (a) applying the ethylenediamine-N,N' disuccinic acid as bioactive chelating agent for the microelements ( iron, zinc, cobalt, copper, manganese, magnesium) and the oxyacids as the second ligands for complexes formation, (b) applying of these monocomplexes and compositions of several metal complexes for the treatment of plants to stimulate the growth of productivity of aforenamed plants in range of 20 till 50%, for curing the first and second phases of chlorosis in full and of curing and stimulating of vital activity of animals and poultry.
DETAILED DESCRIPTION OF THE INVENTION
The improved method of the plant treatment started with the synthesis of corresponding chelate compounds which comprises a chemical reactions of relative metal salts with the ehtylenediamine-N,N'-disuccinic acid (edds) tetrasodium/tetrapotassium salts Na4K4edds) in water solution ( and corresponding oxyacids).
The synthesis can be carried out in two steps:
a. Synthesis of sodium/potassium salt of edds performed by dissolving malefic acid or malefic anhydride in water in the ratio of l:l by weight foe malefic acid and with the ratio of 1:1.5 for malefic anhydride. To this solution the calculated amount of sodium potassium hydroxide have to be added slowly with the small portions to prevent the boiling and foaming of the reactive mass. To this solution the calculated amount of ethylenediamine (70% water solution or 60 % water solution) adding dropwise under active stirring and at the temperature of no more then 50-60 degrees Celsius, till all ethylendiamine would adding to reactive mass. Reactive vessel should be provided with the downward condenser and temperature control facilities. After addition of all ethylenediamine the temperature should be increased to 105-110 degrees of Celsius and the pH of the solution must be in the range of 10.5 till 11.5. The reaction should run for 20-24 hours at the temperature of 105-110 degrees Celsius and under active agitation.
After reaction completed the reactor cooled and thick slightly yellow solution of edds salt removed and precipitated with the adding hydrochloric acid at temperature of 0-15 degrees Celsius. After filtering and drying of the precipitation the white powder of edds ( 90-95% yield) obtained.
The calculated amount of edds putting in reaction with appropriate amount of NaOH
or KOH to receive the tetra sodium/tetrapotassium salt of edds, in water solution containing double amount of water to compare with the summarizing weight of reactives.
b. Metal nitrate or chloride salt dissolved in the minimum amount of the water, enough to receive the clear solution and start adding to the edds solution with the small portions controlling the pH of the solution after each addition of metal salt solution.
At the end of eddition of the metal ions solution the pH of the blend should not be less then 4.9-5.5 and no more then 8Ø These concentrated solutions (may contain the solid particles) can be used directly, after chemical analysis, or can be dryed under 90 degrees Celsius to obtain dry materials.

To receive the complexes with two ligands, the monoligand complexes mixed with the oxyacid in the ratio 1:1 in water solution and heating to 45-50 degrees of centigrade, under agitation for 1-2 hours.
Then the complexes can be precipitated or used directly to obtain desirable blends.
For the cases of Fe, Mn, Mg, Co, Cu ions, compounds in solid state and in solution are dimeric and containing 2-3 molecules of water. In the cases of complexes with edds and citic, tartaric acids complexes are monomeric and containing 1-2 molecules of water bounded to the active groups of ligands ( for Mn the structure is more complicated).
For the treatment of plants, the following solutions have to be prepared by diluting the concentrate solutions, or by dissolving the solid compounds in the water to obtain the following concentrations:
Fe - edds 0.01- 0.20(mass % for individual of Me) complex Mn - edds 0.01- 0.10(mass % for individual of Me) complex Zn - edds 0.01- 0,25(mass % for individual of Me) complex Cu - edds 0.01- 0.10(mass % for individual of Me) complex Co - edds 0.005- 0.10(mass % for individual of Me) complex Mg - edds 0.05 - 0.20(mass % for individual of me) complex Fe - edds - oxyacid ( 1:1:1 ) 0.01 ( mass % of Fe) for mixed complex Mn - edds - oxyacid ( 1:1:1 ) 0.01 ( mass % of Mn) for mixed complex Fe - edds - boric acid ( 1:1:1 ) 0.01 ( mass % of Fe) for mixed complex Co - edds - oxyacid ( 1:1:1 ) 0.005-0.01 ( mass % of Co) for mix complex Cu - edds - oxyacid ( 1:1:1 ) 0.005-0.01 ( mass % of Cu) for mix complex It has been found that the application of the dilute solutions of complexes consisting with the aforementioned bioactive metals and ethylenediamine-N,N'- disuccinic acid and tartaric, citric and boric acids for the plant treatment give the remarkable results, and can be carried out in following steps.
For the honey planted nectar productive ( polygonum, clover) feeding ( Lucerne, rape, sweetclover, cockhead sainfoin ) technical ( hop, flax ) culture the compositions of monocomplexes of Fe-edds and Zn-edds in the ratio of 1: 0.5-1, compositions of Fe-edds-tartaric acid and Zn-edds with the ratio of 1: 0.5-l, monocomplex of manganese and monocomplex of magnesium { buckwheat) are used for spray-treatment within the plants bud formation period, as the diluted solution containing 0.01-0.2 percents of each metal in solution. The treatments should be done one or two times within the interval of one month. Treatments should be carried out at early morning, following by the sunny, dry day lasting for at least 8-12 hours. For this period the complexes are partly utilized by plants directly or decomposed to the bioactive fragments, which in turn are actively utilized by foliage. The consumption of the stimulators for these cultures is 2.5-3.0 kg per hectare. The amount of spraying solutions is 800-1200 L per hectare.
The effect consists of increasing the both seed cropping harvest up to 40-50 %
and nectar productivity up to the 100%.

For viti- and horticultures the compositions of iron with the edds and citric acid, iron with the edds and tartaric acid and iron with the edds and boric acid, mixed compositions of iron, copper, zinc and cobalt with the edds in the ratio of 1: 0.2 : 0.1 :
0.05 are used to increase the grapes and fruits crop harvesting up to the 30-40%, fruits and grapes sugar content up to the 20%.
These formulations also completely cures the first and second phases of chlorosis and reduce the effect of third phase of chlorosis as well.
The treatments performed by spraying the plants within the period of first leaf appearance and performed for two-three times with the interval of one month. The consumption of the complexes are 5- 10 kg per hectare. The amount of spraying solutions is per hectare.
The treatment can be carried out by root application method and in this case, the pronounced prolonged effect occurs: the crop harvest increased up to 20-25%
next year without any treatment.
The consumption of the complexes for root treatment is 10-30 kg per hectare.
Complexes can be introduce to the soil with the macro- fertilizes directly.
For the floriculture the compositions of iron edds and magnesium edds in the ratio of 1:0.5, the compositions of iron, manganese, copper with the ethylenediamine-N,N'-disuccinic acid and citric acid ( 1:1 ) in the ratio of 1:0.2: 0.2 are applying as quantity increasing agents for decorative flowers ( roses, pink-flowers, herberas, etc.) cultivated both in greenhouses and in an open ground. These compositions can be applied for spray-treatment and for root-treatment, that enables to receive the stem thickening, increasing the amount of flower stems, to increase the elite product sorts up to 25-35 %.
The application should be done by treating the flowers once a month starting from the moment of bud formation for all period of growing the flowers.
The consumption of these compositions are 35-50 kg per hectare for greenhouses treating performing all year round).
The amount of solution for spraying is 400-500 L per hectare per treatment.
In vegetable production the compositions of iron, zinc, cobalt and magnesium with the edds and iron with the edds and tartaric acid, iron with the edds and boric acid are used to increase the fruits crop harvesting in open ground and in greenhouses. The methods of treatments are the same as previously mentioned: spraying and root feeding. In the event of spraying, the dilute solution with the concemration of bioactive compositions of 0.01-0.15 mass percentage of each metal, spraying onto the foliage once a month.
It have been found, that the application of these compounds and compositions increasing the total crops harvest up to 30-35 %, stimulates the plant's vitality, completely cures the ferruginous and magnesium chlorosis. The shoot growth intensification is attained in two-three weeks.
The consumption of the compounds is 4-4.5 kg per hectare. The total amount of solution is 600-1200 liters per hectare.
The complexes of iron-edds and iron edds, citric acid, have been found to be very effective for the poultry farming as a hemoglobin level increasing agent, which gives rise to poultry resistance to diseases and stress situations. The method of treatment is to add the complexes to the poultry food once a day through all period of growth. The application of these compounds leads to the increasing the poultry weight up to the 50-80 and reducing the broilers cramming time in 20-35 %.
The consumption of these complexes is 30-60 mg / kg per day.
There were no toxic effects occurred during the 3 years of testing period.
The compositions of 25-50% of iron edds, 10- 25 % of cobalt edds, 10-20 % of copper edds and 5-15 % of manganese edds complexes were used to treat the fur animals grown in cages. It was estimated, that this treatment completely exclude stress and white-puff development in animals, increasing the rate of fur growth, improves fur quality. Special effect were occurred, when the animals are fish fed.
The consumption of the compositions is 55-200 mg/kg per day. The compositions should be added to food or to water.
The effect of increasing the off springs up to 15-25 % occurred while treating foxes, sables and minks.
In the aforesaid discloser it is to be understood that the term "edds" is used to define either ethylenediamine-N,N"-disuccinic acid , or corresponding anion of this acid.
The critical feature of these treatments, as stated hereinbefore, is the application of coordination compounds of iron, manganese, copper, cobalt, zinc and magnesium with ethylenediamine-N,N'-disuccinic acid and tartaric, citric and boric acids, as the second ligands, to increase the crops harvesting of seeds, fruits, green masses, nectar production, to cure chlorosis of the plants at first and second phases completely and the third phase partly, for prophylaxis and veterinary treatment of fur animals and some pets, stimulating agents in poultry farming.
PRESENTLY PREFERRED MODES OF OPERATIONS OF
INVEVTION
Having described the basic concepts of the instant invention, reference is now made to the following examples which illustrate the presently performed embodiments of the invention.

Application of Fe, Zn - edds complexes for the honey planted nectar-productive plants of Lucerne of Jigeva 118 sort.
During the bud formation period (May) the spraying of foliage of Lucerne was performed on the standard background. Thirty lots of 30 sq. m. were used for tests. Five of them were used as control lots. The non-root feeding by complex compositions were performed according the scheme:
a. Control (water and basic agricultural background + inorganic salts of iron sulfate and zinc sulfate).

b. Fe, Zn 0.01 ( mass% Fe) , )Ø 005 adds- ( mass % Zn) c. Fe, Zn 0.05 ( mass% of each metal) --adds-d. Fe, Zn 0.10 ( mass% of each metal) -edds-e. Fe, Zn 0.15 ( mass% of each metal) -edds-f. Fe, Zn 0.20 ( mass% of each metal) -adds-The amount of solution for each lot was estimated as 25-30 liters per each lot.
The effectiveness was estimated for nectar production, seed formation and the amount of green mass of plants The results of seed cropping, nectar production and green mass harvesting tests for Lucerne are presented in the Table 1, 2.
Table 1. Seeds harvesting and green mass productivity of Lucerne of Jigeva 118.
Type of Content of Seeds harvestAdditional Green mass Additional treatment mass% Fe; centners / increasing harvest increasing (Zn) ha in % centn./ha in a - 4.49 - 420.6 -b 0.01' 0.005 6.21 38 481.3 14.5 c 0.05 each 6.07 34 492.0 17.0 d 0.10 each 6.70 49 532.8 27.0 a 0.15 each 6_.30 40 515.1 22.5 f _ _ 5.98 ~ 1 489.0 16.5 0.20 each Calculations of seeds amounts were performed after threshing the full ripening of Lucerne.
Table 2. Nectar productivity in Lucerne of Jigeva 118.
Type Content Amount Amount % of Amount ProductiIncrease of treatmenof mass%of stemsof increasinof sugarson of of sugars t Fe; (Zn)on squ.flowers g of in nectarsugars In %
in to m. on stem flowersmg/flow.nectar control in k a a - 176 246 - 0.204 88.0 -b 0.01(0.00175 260 8 0.341 155.0 77 c 0.05 176 385 50 0.258 174.5 98 d 0.10 175 437 80 0.324 248.0 182 a 0.15 176 455 85 0.290 290.5 163 _ rf - ~ 0.20 I 175. 325 ~ 35. x.321 184.5 ( 109 - ~ I ~ --~

The determination of nectar in flowers were performed on the base of whole removal of nectar from flowers by washing with water and, consequently, determining the glucose fructose content by conventional methods.

The influence of stimulators was investigated for the pink-flowers and for roses during 9 years and showed the excellent results. Table 3 presents some data of 3- years cycle of treated pink-flowers in greenhouses by the composition of Fe, Cu, Mn, Mg -edds -tartaric acid complexes and by non-root spraying- treatment with the 0.015%
solution for the flowers ( Fe : Mn : Mg : Cu = 1 : 0.2 : 0.2 : 0.1 ).
Table 3. Application of bioactive complexes for the pink-flowers ( data for 1 sq. m.) Type QualityAmount Amount Amount IncreaseIncreaseIncrease of of of of steamsof of stemsof stemsof stems test steams steams steams amount amount amount 1~ ear 2 ear 3 ear 1 ear 2 ear 3~ ear Control extra 153 162 lb4 - - -Treated e~ 165 ~ 179 - 182 12 - 17 18 ~ _ I -.

At the Table 4, the results of application of the same compositions for the roses in greenhouses for 3-years cycle are presented.
Table 4. Data for the application of stimulators for the roses ( data for 1 sq. m. ) QualityAmount Amount Amount IncreaseIncreaseIncrease of stemsof stemsof stemsof stemsof stemsof stemsof stems amount amount amount 1~ ear 2 ear 3 ear 1~' ear 2n ear 3~ ear Control e~ _ 69 - 71. _ _ 70 - - - _ _. __ Treated-~ e ~-97 101 - 102 I 2g--~ 30 32 -- I _ ~ -_..

These experiments were performed three times for 3-years cycles and gave the similar results (differences were 5-10% in amount of steams per square m).
It have been shown, that after treatment of flowers with the stimulators, the amount of extra quality stems increasing up to 25-40%.

For the experimental treatment, two areas of 50 hectares of viticulture of "Sauvignon"
type were used Treatment were performed by non-root spraying with the amount of spraying solution of 400-600 liters of solution per hectare. Concentration of the stimulator was 0.015 % of Fe.
The first spraying was performed during the first leaves formation period, the second - in one month and the third- at the end of third month.
After the grapes ripened, the total crop harvesting were calculated and the amount of sugars in grapes were estimated.
It appeared that the application of the Fe-edds-tartaric acid formulations for the grapes gave the increase of crops in 30-40% and the increase in sugars content in 20-25%.

Application of the magnesium complex of edds for the buckwheat.
Twenty four lots of buckwheat with the similar area and agricultural background were used for the tests.
Treatments were performed as non-root spraying of dilute solutions of complexes and sulfate of magnesium as control, during the vegetation period of buds formation.
Treatments were done two times in a period of one month.
The following scheme of treatment were chosen:
a. Control 0.1% of sulfate of magnesium b. Fe-edds 0.01 % solution ( Fe mass % ) c. Mg-edds 0.005 % solution ( Mg mass %) d. Mg-edds 0.010 % solution ( Mg mass %) e. Mg-edds 0.015 % solution ( Mg mass %) The data are represented in the Table 3 and Table 4.
Table 3. The seeds crop of buckwheat after non-root treatment with the stimulators.
Variants Crops harvest Addition to Addition to in comrol control cent/ha c/ha in a 19.47 - -b. 27.65 8.18 42 c. 30.72 11.25 58 d. 3 13.96 72

3.43 e. _ __ 68 32.70 T 13.23 Table 4. The nectar productivity of buckwheat after complex's treatments.
Variants Amount Amount Amount ProductiviAddition Addition of of of plants flowers sugar ty of to controlto control, on on in sugar one sq. one plantnectar in nectarkghla m. for 1 flower kg/ha in m a. 84 1098 0.124 114.4 - -b. 84 1120 0.155 145.6 31.4 29 c. 84 1099 0.153 141.0 26.6 23 d. 84 1117 0.154 144.6 30 26 .2 ~4 ~ 1057 j 0.169 ( 150.0 _ 31 ~5.6 As it can be easily seen from the tables, non-root treatments of buckvvheat gave the increasing of the seeds crops harvesting up to the 70%, nectar productivity -up to the 30 %, which are sufficient for this culture.
As will be apparent to one skilled in the art, various modification and, consequently, application can be made within the scope of the aforesaid description.
Suchmodifications being within the ability of one skilled in the art form a part of the present invention and are embraced by the appended claims.

Claims

The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows:

1. A class of chemical compounds named the complexonates of ethylenediamine-N,N'-dicarbonic acids with the bioactive metals of iron, zinc, manganese, magnesium, cobalt and copper and polyligand complexes of aforenamed complexonates with boric acid and oxyacids, like tartaric, citric, ascorbic acids, and their compositions with the different content of every metals held in the range of 1% till 100% of each particular metal ion complexes, and which are used as microfertilizes and stimulators of growth, increasing the crops harvesting of seeds, fruits, flowers, nectar productivity of plants; curing agents for the treatment of chlorosis of plants.

2. The methods of treatment of the plants in the period of bud formation, first leaves formation vegetation period of flowers growth, wherein the plants are treated by non-root spraying of the foliage by the dilute solutions of the claim 1 compounds in the concentration range of Fe - complexonate ~0.01 - 0.20 (mass% of Me) for individual complex Mn - complexonate 0.01 - 0.10 (mass% of Me) for individual complex Zn - complexonate 0.01 - 0,25 (mass% of Me) for individual complex Cu - complexonate 0.01 - 0.10 (mass% of Me) for individual complex Co - complexonate 0.005- 0.10 (mass% of Me) for individual complex Mg - complexonate 0.05 - 0.20 (mass% of Me) for individual complex Fe - complexonate - oxyacid (1:1:1) 0.01 - 0.15 (mass % of Fe) for mixed complex Mn - complexonate - oxyacid (1:1:1) 0.01 - 0.15 (mass % of Mn) for mixed complex Fe - complexonate - boric acid (1:1:1) 0.01- 0.15 (mass % of Fe) for mixed complex Co - complexonate - oxyacid (1:1:1) 0.001 - 0.01 (mass % of Co) for mixed complex Cu - complexonate - oxyacid ( 1:1:1 ) 0.01- 0.15 ( mass % of Me) for mixed complex

3. The method of root treatments of the plants by the compounds and compositions of the claim 1, wherein the stimulators are input to the roots of the plants with the conventional fertilizes.

4. The method of treatments of fur animals and pets, poultry with the chemical compounds of claim 1, wherein the compositions of claim 1 adding to the food or water in the range of 20-100 mg per kg of weight per day in the period of growth of animals or poultry.