AU2004210010B2 - Metal ion chelators and therapeutic use thereof - Google Patents

Description

1 Metal Ion Chelators and Therapeutic Use Thereof Field of the invention The present invention relates to compounds which are capable of chelating metal ions. In particular, the present invention relates to (thio)semicarbazone compounds and (thio)hydrazone 5 compounds which are capable of chelating metal ions, including iron ions. The invention also relates to therapeutic use of such compounds and/or their metal ion complexes, including methods of treating diseases associated with cell proliferation. Background of the invention Iron (Fe) is fundamentally involved in many important cellular processes. For example, 10 Fe-containing proteins catalyse key reactions involved in energy metabolism, respiration, and DNA synthesis. Cellular Fe-deprivation results in G 1 /S arrest and apoptosis.2 Tumour cells are far more sensitive than normal cells to iron depletion possibly due to their increased rates of proliferation. The increased iron requirements of tumour cells is reflected by the increased expression of the Transferrin Receptor I which binds the serum Fe-transport protein, transferrin 15 (Tf). Neoplastic cells also express higher levels of the Fe-containing enzyme, ribonucleotide reductase, which is the critical rate-limiting step of DNA synthesis. Cellular iron pools therefore represent an important therapeutic target and compounds which are capable of chelating iron may be useful therapeutic agents in the treatment of iron-associated diseases and disorders, as well as potential anti-tumour agents. 20 Desferrioxamine (DFO) is an iron chelator currently used for the treatment of iron overload disease. However, the use of DFO as an anti-cancer agent is limited by its modest anti-proliferative activity. There is a need for therapeutically acceptable metal ion chelators, including iron chelators, which are capable of permeating cell membranes and chelating intracellular Fe. 25 There is also a need for clinically useful iron chelators having anti-proliferative activity. Summary of the Invention A first aspect of the invention disclosed herein relates to a compound of Formula 1 A N E B N R' G WO 2004/069801 PCT/AU2004/000132 2 Fonnula 1 wherein E is O or S; A is (')M0 ~N 5 11Z. 0 wherein Z', Y', X' and W' are independently selected from CR 4 , NR 8 , S and 0, wherein the total number of heteroatoms in A is 1, 2 or 3, m is 0 or 1, B is IV \ (X)q Z 10 Y wherein Z, Y, X, W and V are independently selected from CR 4 , NR 8 , S and 0; and wherein the total number of heteroatoms in B is 0, 1, 2, or 3; q is 0 or 1; each R4 is independently selected from hydrogen, halogen, alkyl, alkenyl, optionally 15 substituted amino, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, -C(O)-alkenyl, -C(0)-0 alkyl, nitro and cyano; each R 8 is independently selected from hydrogen, halogen, alkyl, alkenyl, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-allcyl, and -C(O)-alkenyl, benzyl, benzylcarbamate; R' is hydrogen, or a group which does not hinder metal ion chelation; 20 -G is NR 2

R

3 or CR 2

R

3

R

5 , wherein the bond between C and R 5 may be a single bond, double bond, or triple bond; and wherein when the bond between C and R 5 is a double bond, one of R 2 and R 3 is absent, and wherein when the bond between C and R 5 is a triple bond, R 2 and R 3 are both absent; 25 R 5 is selected from the group consisting of hydrogen, halogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted amino, optionally substituted heteroaromatic, an optionally substituted bicyclic group, and an optionally substituted aromatic group;

R

2 and R 3 may be the same or different and are individually selected from 30 hydrogen, halogen, optionally substituted alkyl, optionally substituted alkenyl, optionally WO 2004/069801 PCT/AU2004/000132 3 substituted alkynyl, optionally substituted amino, optionally substituted heteroaromatic, an optionally substituted bicyclic group, and an optionally substituted aromatic group; or -G is an optionally substituted heteroaromatic group, an optionally substituted bicyclic group, an optionally substituted aromatic group, an optionally substituted alkyl 5 group; an optionally substituted cycloalkyl group, or an optionally substituted heterocycloalkyl group; with the provisos that: (i) when E is 0, and A and B are each (N% then 10 -G is not -NH 2 , -CH 2 Cl, N CH 3 -NH-N N-NH N-NH S\

>-NH

2 -- NH2 o S B B Brr BrP C1 Br, iF F F NH2 OH H N HO N0N NNO N, HO N N N N and (ii) when E is S, and A and B are each 20 , then WO 2004/069801 PCT/AU2004/000132 4 -G is not -NH 2 , -NH -NH HO H iHH -N -N, N -N /

CH

3 , N -NH-N OH -NH-N:/ -NH-N

,CH

3

/CH

2

-CH

3 /(CH2)2-CH 3

N(CH

2

)

3

-CH

3

N(CH

2

)

4

-CH

3 5 CH3, CH2-CH3,

(CH

2

)

2

-CH

3

(CH

2

)

3

-CH

3 , (CH 2

)

4

-CH

3

OH

3 (CH2)5-CH3 -N O -N 0 (C -N -NN O_ -N N-CH3 -NH (CH2) 5

-CH

3 , , \-N,-O , OH 3 ,-N S -N N - HN-N H 3 H N-N N C N H 3 S NZ 10 HO or With reference to the first aspect of the invention A and B may be the same or different. In one embodiment, A and B are the same. In another embodiment A and B are different. 15 In some embodiments, A may be selected from optionally substituted pyridyl, optionally substituted pyrimidinyl, optionally substituted triazinyl, optionally substituted oxazolyl, and optionally substituted pyrrolyl. In alternate embodiments, B may be selected from an optionally substituted 5- or 6 membered aromatic ring and an optionally substituted 5- or 6-membered heteroaromatic 20 ring. For example, B may be selected from an optionally substituted phenyl, naphthyl, pyridyl, pyrimidinyl, triazinyl, oxazolyl, pyrrolyl, and furanyl group. Optional substituents may include electron withdrawing groups and electron donating groups. Examples of optional substituents include alkyl, alkenyl, alkynyl, C 6

.

10 aryl, halo, amino, 5 hydroxyl, 0-alkyl, S-alkyl, nitro, cyano, C(O)-alkyl, C(O)-O-alkyl, C(O)-NH(alkyl), and C(O)-N(alkyl) 2 . In one embodiment, R' is hydrogen. In alternate embodiments, when E is 0,-G is selected from optionally substituted 5 -C 1 .3 alkyl, -C 2 -8 alkenyl, aryl, heteroaryl, -CH 2 (aryl), and -CH(aryl) 2 . In other embodiments, when E is S, -G is selected from optionally substituted -C 1 - alkyl, -C 2 -8 alkenyl, -CH 2 (aryl), -CH(aryl) 2 , aryl and heteroaryl. In a further embodiment, when E is S, -G is selected from optionally substituted -NH(C 2 -6-alkenyl) and -N(aryl) 2 . A second aspect of the invention disclosed herein relates to a compound of Formula 2: A >=E N, E B HN 10 G Formula 2 wherein A is selected from: N N- N N N (R4)n rr-N (R4)n 1(R4)n (R)-( N and H wherein each R 4 is independently selected from halogen, alkyl, alkenyl, optionally 15 substituted amino, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, -C(O)-alkenyl, -C(O)-O-alkyl, nitro and cyano, n is 0, 1, 2,3 or4; and wherein B, E and G are as defined for Formula 1, including provisos (i) and (ii). In one embodiment, A is <N (R4)n 20 wherein each R 4 is independently selected from halogen, alkyl, alkenyl, optionally substituted amino, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, -C(O)-alkenyl, -C(O)-O- alkyl, nitro and cyano, and nis0, 1,2, 3or4. 25 In one embodiment, B is selected from 6 4) (R (R4)p-

(R

4 ) (R

(R

4 )p 4)p H and S wherein each R 4 is independently selected from halogen, alkyl, alkenyl, optionally substituted amino, hydroxyl, -0-alkyl, -S-alkyl, -C (0)-alkyl, -C (0)-alkenyl, -C(O)-O-alkyl, nitro 5 and cyano, and p is an integer from 0 to 7, wherein A, E and G are as defined in Formula 1, including provisos (i) and (ii). In one embodiment, p is 0, 1, 2, 3 or 4. In one embodiment, B is (R4)p 10 4 N According to a third aspect disclosed herein, the invention provides a compound of Formula 3:

(R

4 )n -N E N G or a pharmaceutically acceptable salt thereof, 15 wherein EisOorS; n is0, 1,2, 3or4; pis0, 1,2, 3or4; each R 4 is independently selected from halogen, alkyl, alkenyl, optionally substituted 20 amino, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, -C(O)-alkenyl, -C(O)-O-alkyl, nitro and cyano; -G is NR 2

R

3 or CR 2

R

3

R

5 , wherein the bond between C and R 5 may be a single bond, double bond or triple bond; and wherein when the bond between C and R 5 is a double bond, one of R 2 and R 3 is absent; and wherein when the bond between C and R 5 is a triple bond, R 2 and R 3 are both absent; 25 R 2 and R 3 may be the same or different and are individually selected from hydrogen, halogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, 6a optionally substituted amino, optionally substituted heteroaromatic, an optionally substituted bicyclic group, and an optionally substituted aromatic group; and

R

5 is selected from the group consisting of hydrogen, halogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted amino, 5 optionally substituted heteroaromatic, an optionally substituted bicyclic group, and an optionally substituted aromatic group; or G is an optionally substituted heteroaromatic group, an optionally substituted bicyclic group, an optionally substituted aromatic group, an optionally substituted alkyl group, an optionally substituted cycloalkyl group, or an optionally substituted heterocycloalkyl group; 10 with the provisos that: (i) when E is 0, n is 0 and p is 0, then -G is not -NH 2 , CH 2 CI, 2-furanyl, 2-thiophenyl; phenyl, 2-halophenyl, 3-halophenyl or 4-halophenyl, where halo is F, Cl, Br or 1; 2-aminophenyl; 4-aminophenyl, 2-hydroxyphenyl; 4-hydroxyphenyl; 2-pyridyl; 4-pyridyl; N H

-

CH

3 -N-N \4 N-NH N-NH N' 0 NH 2 0-NH2 15 - ,0 and (ii) when E is S, n is 0 and p is 0, then -G is not NH 2 , NHCH 3 , -NHPhenyl, -NH(benzyl); NH(benzyl) 2 ; -NH(2-pyridyl); -NH-CH 2 -(2 pyridyl); -N(C-C 6 alkyl) 2 N(methyl) 2 ;N(ethyl) 2 ;N(propyl) 2 ;N(butyl) 2 ;N(pentyl) 2 ;N(hexyl) 2 ; 20 N(methyl)phenyl; -N-pyrrolidinyl; -N-piperidinyl; -N-morpholinyl; 2-thiophenyl; 2-furanyl; 2-hydroxyphenyl; N

OH

H -N-N H H H -N-N . H H -N N N -N-N, -N-N - 6b

-

-\ N S -N N

CH

3 HN-N -N O -- O - N -N N-CH 3 N C33 or C H OH 3 H N 'N

CH

3 S N and (iii) when E is S, n is 0 and p is 1, then: (a) when R 4 is 6-Br, G is not N(methyl) 2 ; 5 (b). when R 4 is 6-methyl, G is not N(methyl) 2 ; and (c) when R 4 is 6-F, G is not N-pyrrolidinyl. According to a fourth aspect of the invention there is provided a compound of Formula 4: WO 2004/069801 PCT/AU2004/000132 7 N -- N \ E - HNI IN G Formula 4 wherein E is O or S; and 5 -G is as defined above for Formula 1, including provisos (i) and (ii). In one embodiment of the invention, when E is 0, the compound of Formula 4 is di 2-pyridylketone 4,4-diphenylsemicarbazone (abbreviated PK44pH). In another embodiment, when E is 0 the compound of Formula 4 is di-2-pyridylketone octanoic hydrazone (abbreviated PKoctH). 10 In a further embodiment of the invention, when E is S, the compound of Formula 4 is di-2-pyridylketone 4-ethyl-3-thiosemicarbazone (abbreviated Dp4eT). In another embodiment, when E is S the compound of Formula 4 is di-2-pyridylketone 4-allyl-3 thiosemicarbazone (abbreviated Dp4aT). Compounds of Formula 1, 2, 3 or 4 may be capable of chelating metal ions. 15 Accordingly, in another aspect of the invention there is provided a metal ion complex of a compound of Formula 1, 2, 3 or 4 as defined in any one of the first to fourth aspects of the invention, including or excluding provisos (i) and (ii). In one embodiment, the metal ion complex of a compound of Formula 1, 2, 3 or 4 may be a transition metal ion complex, such as copper, iron, zinc, palladium, platinum, or 20 gallium ion complex. In one embodiment, the metal ion is iron(II). In another embodiment, the metal ion is iron(III). In one embodiment, compounds of Formula 1, 2, 3 or 4 may function as tridentate ligands. In one embodiment, two compounds of Formula 1, 2, 3 or 4 may form a hexacoordinate complex with a metal ion, for example, iron(II) or iron(III). 25 According to a fifth aspect of the invention there is provided a pharmaceutical composition comprising a pharmaceutically acceptable diluent, adjuvant or carrier together with at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii). In one embodiment, the pharmaceutical composition of the invention comprises at 30 least one compound of Formula 1, 2, 3 or 4 according to any one of the first to fourth aspects of the invention, including provisos (i) and (ii).

WO 2004/069801 PCT/AU2004/000132 8 In some embodiments of the invention, the pharmaceutical composition may comprise one or more metal ion complex of a compound of Formula 1, 2, 3 or 4. Suitable metal ions include transition metal ions. For example, the metal ion may be iron(II), iron(III), copper(II), zinc(II), palladium(II), platinum(II), or gallium(III). 5 Compounds of Formulae 1, 2, 3 and 4 as defined herein with or without provisos (i) and (ii), and compositions thereof, may be used for in vitro or in vivo metal ion chelation. In one embodiment, compounds of Formulae 1, 2, 3 and 4 as defined herein with or without provisos (i) and (ii), and compositions thereof, may be used for iron chelation. Accordingly, compounds of Formulae 1, 2, 3 and 4 as defined herein with or without 10 provisos (i) and (ii), and compositions thereof, may be suitable for iron chelation therapy or treating iron-overload disorders. Compounds of Formulae 1, 2, 3 and 4 as defined herein with or without provisos (i) and (ii), and compositions thereof, may also be used for modifying cellular function by chelating cellular metal ions, including iron. For example, compounds of Fonnulae 1, 2, 3 and 4 as defined herein with or without provisos 15 (i) and (ii), and compositions thereof, may be useful for inhibiting cellular proliferation and/or inducing apoptosis. Compounds of Formulae 1, 2, 3 and 4 as defined herein with or without provisos (i) and (ii), and compositions thereof may also be useful for modifying expression of genes which are sensitive to iron concentration. Examples of such genes include WAF1, GADD45, and Ndrgl. 20 Pharmaceutical compositions according to the invention may be formulated for subcutaneous or intravenous injection, oral administration, inhalation, transdermal application, or rectal administration. In one embodiment of the invention the composition is formulated for intravenous administration. In another embodiment the composition is formulated for oral administration. 25 According to a sixth aspect of the invention there is provided a method of iron chelation therapy in a mammal comprising administering to said mammal a therapeutically effective amount of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a pharmaceutical composition comprising at least one compound of Formula 1, 2, 3 or 4 with or without provisos (i) and 30 (ii) together with a pharmaceutically acceptable diluent, adjuvant, or excipient. According to a seventh aspect of the invention there is provided the use of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), for the preparation of a medicament for iron chelation therapy. According to an eighth aspect of the invention there is provided a method of 35 treating an iron-overload disorder in a mammal comprising administering to said mammal WO 2004/069801 PCT/AU2004/000132 9 a therapeutically effective amount of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a pharmaceutical composition comprising at least one compound of Formula 1, 2, 3 or 4 with or without provisos (i) and (ii) together with a pharmaceutically acceptable diluent, adjuvant, or excipient. 5 According to a ninth aspect of the invention there is provided the use of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), for the manufacture of a medicament for treating an iron-overload disorder. With reference to the eighth and ninth aspects of the invention, in one embodiment the iron overload disorder is p-thalassaemia. 10 According to a tenth aspect of the invention there is provided a method of inhibiting cellular proliferation comprising exposing a cell to an effective amount of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a metal ion complex thereof, or a pharmaceutical composition comprising at least one compound of Formula 1, 2, 3 or 4 with or without provisos (i) and (ii), or a metal ion is complex thereof together with a pharmaceutically acceptable diluent, adjuvant, or excipient. With reference to the tenth aspect of the invention, in one embodiment, compounds of Formulae 1, 2, 3 and 4, metal ion complexes thereof, or compositions comprising said compound or metal ion complex may be used to inhibit in vitro cellular proliferation. In 20 another embodiment, compounds of Formulae 1, 2, 3 and 4, metal ion complexes thereof, or compositions comprising said compound or metal ion complex may be used to inhibit in vivo cellular proliferation, for example, cellular proliferation in a mammal. According to an eleventh aspect of the invention there is provided a method of treating a proliferative disorder in a mammal comprising administering to said mammal a 25 therapeutically effective amount of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a metal ion complex thereof, or a pharmaceutical composition comprising at least one compound of Formula 1, 2, 3 or 4 with or without provisos (i) and (ii), or a metal ion complex thereof together with a pharmaceutically acceptable diluent, adjuvant, or excipient. 30 According to a twelfth aspect of the invention there is provided the use of at least one compound according to Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a metal ion complex thereof, for the manufacture of a medicament for inhibiting cellular proliferation. According to a thirteenth aspect of the invention there is provided the use of at least 35 one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and WO 2004/069801 PCT/AU2004/000132 10 (ii), or a metal ion complex thereof, for the manufacture of a medicament for treating a proliferative disorder. With reference to the eleventh or thirteenth aspects of the invention, the proliferative disorder may be selected from angiogenesis-dependent diseases, cellular 5 proliferative diseases (such as psoriasis, IIBD, malignancies, restenosis), inflammatory disorders, auto-immune diseases, blood vessel diseases, thrombosis, cancer. The proliferative disease may be arthritis. The cancer may be a malignant tumour or benign tumour. The malignant tumour may be metastatic. The cancer may be a solid tumour or a non-solid tumour. For example, the cancer may be a leukaemia or lymphoma. 10 In one embodiment, when the compound or composition of the invention is administered as the sole active agent the resultant white blood cell count in the mammal is changed, and in particular is reduced, by no more than 1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85% following administration of the compound or composition. Similarly, in one embodiment is when the compound or composition of the invention is administered as the sole active agent the resultant red blood cell count is changed, and in particular is reduced, by no more than 1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85% following administration of the compound or composition. 20 According to a fourteenth aspect of the invention there is provided a method of inducing apoptosis in a cell, said method comprising contacting said cell with an effective amount of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a metal ion complex thereof, or a pharmaceutical composition comprising at least one compound of Formula 1, 2, 3 or 4 with or without provisos (i) and 25 (ii), or a metal ion complex thereof together with a pharmaceutically acceptable diluent, adjuvant, or excipient. According to a fifteenth aspect of the invention there is provided a method of inhibiting cellular proliferation and inducing apoptosis in a cell, said method comprising contacting said cell with an effective amount of at least one compound of Formula 1, 2, 3 30 or 4 as defined herein with or without provisos (i) and (ii), or a metal ion complex thereof, or a pharmaceutical composition comprising at least one compound of Formula 1, 2, 3 or 4 with or without provisos (i) and (ii), or a metal ion complex thereof together with a pharmaceutically acceptable diluent, adjuvant, or excipient. According to a sixteenth aspect of the invention there is provided a method of 35 inducing apoptosis in a mammal, said method comprising administering to said mammal WO 2004/069801 PCT/AU2004/000132 11 a therapeutically effective amount of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a metal ion complex thereof, or a pharmaceutical composition comprising at least one compound of Formula 1, 2, 3 or 4 with or without provisos (i) and (ii), or a metal ion complex thereof together with a 5 pharmaceutically acceptable diluent, adjuvant, or excipient. According to a seventeenth aspect of the invention there is provided a method of inducing apoptosis and inhibiting cellular proliferation in a mammal, said method comprising administering to said mammal a therapeutically effective amount of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and 10 (ii), or a metal ion complex thereof, or a pharmaceutical composition comprising at least one compound of Formula 1, 2, 3 or 4 with or without provisos (i) and (ii), or a metal ion complex thereof together with a pharmaceutically acceptable diluent, adjuvant, or excipient. According to an eighteenth aspect of the invention there is provided the use of at 15 least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a metal ion complex thereof, for the manufacture of a medicament for inducing apoptosis. According to a nineteenth aspect of the invention there is provided the use of at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) 20 and (ii), or a metal ion complex thereof, for the manufacture of a medicament for inducing apoptosis and inhibiting cellular proliferation. According to a twentieth aspect of the invention there is provided at least one compound of Formula 1, 2, 3 or 4 as defined herein with or without provisos (i) and (ii), or a metal ion complex thereof, or a pharmaceutical composition comprising at least one 25 compound of Formula 1, 2, 3 or 4 with or without provisos (i) and (ii), or a metal ion complex thereof together with a pharmaceutically acceptable diluent, adjuvant, or excipient, when used for any one or more of inhibiting cellular proliferation, treating a proliferative disorder, treating cancer, inducing apoptosis, iron chelation therapy, or treating an iron-overload disorder. 30 According to a twenty-first aspect of the invention there is provided a method of treating or inhibiting a condition selected from the group consisting of cellular proliferative disorder, cancer, iron-overload disorder in a mammal, or inducing apoptosis in a mammal, or inducing apoptosis and inhibiting cellular proliferation in a mammal, said method comprising administering to said mammal a therapeutically effective amount 35 of at least one compound of Formula 1, 2, 3 or 4, with or without provisos (i) or (ii), or an 12 iron complex thereof, or a composition comprising said compound or complex together with a pharmaceutically acceptable adjuvant, diluent or excipient, wherein the resultant white and/or red blood cell count in the mammal is changed, and in particular is reduced, respectively by no more than 1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 5 70%, 75%, 80%, or 85% following administration of the compound or composition to said mammal. With reference to any one of the fifth and tenth to twenty-first aspects of the invention, the metal ion may be a transition metal ion. For example, the metal ion may be selected from iron, copper, zinc, palladium, platinum, and gallium ions. In one embodiment the metal ion is iron (11). 10 In another embodiment, the metal ion is iron (111). With reference to any one of the sixth, eighth, tenth, eleventh, sixteenth, seventeenth, and twenty-first aspects of the invention, the mammal may be human, non-human primate, murine, bovine, ovine, equine, caprine, leporine, avian, feline, porcine, or canine. In one embodiment the mammal is human. 15 A twenty-second aspect of the invention disclosed herein relates to a process for identifying an anti-proliferative compound, said process comprising: contacting a cell or cellular extract having a gene encoding one or more cell cycle inhibitors, with a compound capable of chelating iron, determining the level of expression of said gene, determining whether the level of 20 expression of said gene differs in comparison to the expression of said gene in the absence of said compound; and thereby determining whether said compound has anti-proliferative activity. A twenty-third aspect of the invention disclosed herein relates to a process for screening a plurality of compounds to identify one or more anti-proliferative compounds, said process 25 comprising: contacting a cell or cellular extract having gene encoding one or more cell cycle inhibitors, with a plurality of compounds capable of chelating iron, determining whether any of said compounds modify the level of expression of said nucleic acid, and if so, separately determining the level of expression of said gene for each compound, 30 determining whether said level of expression differs comparison to the level of expression of said in the absence of each said compound; and thereby determining whether each said compound has anti-proliferative activity. With reference to the twenty-second and twenty-third aspects of the invention, in one embodiment, expression of the gene is up-regulated. In another embodiment, expression of the 35 gene is down-regulated. In one embodiment the gene expressing cell cycle inhibitors may be WAF1. In another embodiment of the invention, the gene expressing cell cycle inhibitors may be GADD45. In a further embodiment, the gene is Ndrgl.

13 With reference to the twenty-second and twenty-third aspects of the invention, expression of the gene (e.g., mRNA, protein) in the absence of said compound may be separately determined prior to, or after, determining expression of said gene in the presence of said compound. 5 With reference to the twenty-second and twenty-third aspects of the invention, in one embodiment said compound or plurality of compounds is capable of chelating a metal ion. In another embodiment, said compound or plurality of compounds is capable of chelating iron. In a further embodiment, the compound is a compound of Formula 1, 2, 3 or 4 as defined herein, including or excluding provisos (i) and (ii). 10 With reference to any one of the first to twenty-third aspects of the invention, in one embodiment each said compound of Formula 1, 2, 3 or 4 is as defined in any one of the first to fourth aspects of the invention including provisos (i) and (ii). The present invention includes within its scope all isomeric forms of compounds of Formulae 1, 2, 3 and 4, and metal ion complexes thereof. For example, as appropriate, the 15 present invention includes all possible enantiomers, diastereomers, racemates, cis isomers, trans isomers, (R), (S), (+), (-), A and A isomers of the compounds and/or their metal complexes. Any discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is solely for the purpose of providing a context for the present invention. It is not to be taken as an admission that any or all of these matters form part 20 of the prior art base or were common general knowledge in the field relevant to the present invention as it existed in Australia before the priority date of each claim of this specification. Definitions The following are some definitions that may be helpful in understanding the description of 25 the present invention. These are intended as general definitions and should in no way limit the scope of the present invention to those terms alone, but are put forth for a better understanding of the following description. Unless the context requires otherwise or specifically stated to the contrary, integers, steps, or elements of the invention recited herein as singular integers, steps or elements clearly 30 encompass both singular and plural forms of the recited integers, steps or elements. Throughout this specification, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising" will be understood to imply the inclusion of a stated step or element or integer or group of steps or elements or integers, but not the exclusion of any other step or element or integer or group of elements 35 WO 2004/069801 PCT/AU2004/000132 14 or integers. Thus, in the context of this specification, the term "comprising" means "including principally, but not necessarily solely". Those skilled in the art will appreciate that the invention described herein is susceptible to variations and modifications other than those specifically described. It is to 5 be understood that the invention includes all such variations and modifications. The invention also includes all of the steps, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations or any two or more of said steps or features. All the references cited in this application are specifically incorporated by reference 10 herein in their entirety. As used herein, the term "alkyl group" includes within its meaning straight chain or branched chain saturated aliphatic groups having from 1 to 10 carbon atoms, eg, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 carbon atoms, or cyclic saturated aliphatic groups having from 3 to 8 carbon atoms, eg, 3, 4, 5, 6, 7, or 8 carbon atoms, or bicyclic saturated aliphatic groups 15 having 7, 8, 9 or 10 carbon atoms. For example, the term alkyl includes, but is not limited to, methyl, ethyl, 1-propyl, isopropyl, 1-butyl, 2-butyl, tert-butyl, amyl, 1,2 dimethylpropyl, 1,1-dimethylpropyl, pentyl, isopentyl, hexyl, 4-methylpentyl, 1 methylpentyl, 2-methylpentyl, 3-methylpentyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl, 1,2 dimethylbutyl, 1,3-dimethylbutyl, 1,2,2-trimethylpropyl, 1,1,2-trimethylpropyl, 2 20 ethylpentyl, 3-ethylpentyl, heptyl, 1-methylhexyl, 2,2-dimethylpentyl, 3,3 dimethylpentyl, 4,4-dimethylpentyl, 1,2-dimethylpentyl, 1,3-dimethylpentyl, 1,4 dimethylpentyl, 1,2,3-trimethylbutyl, 1,1,2-trimethylbutyl, 1,1,3-trimethylbutyl, 5 methylheptyl, 1-methylheptyl, octyl, nonyl, decyl, cyclopropyl, 2-methylcyclopropyl, cyclobutyl, cyclopentyl, 2-methylcyclopentyl, 3-methylcyclopentyl, cyclohexyl, and the 25 like. The term "alkenyl group" includes within its meaning straight or branched chain unsaturated aliphatic hydrocarbon groups having from 2 to 8 carbon atoms, e.g., 2, 3, 4, 5, 6, 7, or 8 carbon atoms, or cyclic unsaturated aliphatic hydrocarbon groups having from 3 to 8 carbon atoms, e.g, 3, 4, 5, 6, 7or 8 carbon atoms, and combinations thereof, having at 30 least one double bond, of either E or Z stereochemistry where applicable. Examples of alkenyl groups include but are not limited to ethenyl, vinyl, allyl, 1-methylvinyl, 1 propenyl, 2-propenyl, 2-methyl-1-propenyl, 2-methyl-1-propenyl, 1-butenyl, 2-butenyl, 3 butentyl, 1,3-butadienyl, 1-pentenyl, 2-pententyl, 3-pentenyl, 4-pentenyl, 1,3-pentadienyl, 2,4-pentadienyl, 1,4-pentadienyl, 3-methyl-2-butenyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, WO 2004/069801 PCT/AU2004/000132 15 1,3-hexadienyl, 1,4-hexadienyl, 2-methylpentenyl, 1-heptenyl, 2-heptentyl, 3-heptenyl, 1 octenyl, and the like. The term "alkynyl group" as used herein includes within its meaning straight or branched chain unsaturated aliphatic hydrocarbon groups having from 2 to 8 carbon 5 atoms, e.g., 2, 3, 4, 5, 6, 7, or 8 carbon atoms, and having at least one triple bond. Examples of alkynyl groups include but are not limited to ethynyl, 1-propynyl, 1-butynyl, 2-butynyl, 1-methyl-2-butynyl, 3-methyl-1-butynyl, 1-pentynyl, 1-hexynyl, methylpentynyl, 1-heptynyl, 2-heptynyl, 1-octynyl, 2-octynyl, and the like. The term "heteroatom" as used herein refers to any atom other than carbon or 10 hydrogen, including for example, oxygen, nitrogen and sulfur. The term "halide" or "halo atom" as used herein refers to fluoro, chloro, bromo and iodo. The term "amino group", or variants such as "amino" as used herein refers to groups of the form -NR 6

R

7 wherein R 6 and R 7 are individually selected from the group 15 including but not limited to hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, and optionally substituted aryl groups. The tenn "aromatic group", or variants such as "aryl" as used herein refers to single, polynuclear, conjugated and fused residues of aromatic hydrocarbons having from 6 to 14 carbon atoms, e.g., 6, 7, 8, 9, 10, 11, 12, 13, or 14 carbon atoms. Examples of 20 such groups include phenyl, tolyl, biphenyl, naphthyl, phenanthryl, and the like. The term "heteroaromatic group" or variants such as "heteroaryl" as used herein includes within its meaning any 5-16-membered, eg., 5-, 6-, 7-, 8-, 9-, 10-, 11-, 12-, 13-, 14- ,15- or 16-membered monocyclic, bicyclic or tricyclic aromatic group having 1, 2, 3 or 4 heteroatoms. Examples include, but are not limited to pyrrolyl, pyridyl, phenanthryl, 25 quinolyl, isoquinolyl, imidazolyl, thiophenyl, furanyl, purinyl, indolyl, isoindolyl, phenanthridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, benzofuranyl, and the like. The term "heterocycloalkyl group" as used herein includes within its meaning any 3-10-membered saturated or unsaturated monocyclic or bicyclic ring system, having 1, 2 30 or 3 heteroatoms. Examples include but are not limited to piperidinyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydropyrrolyl, morpholinyl, tetrahydrothienyl, and the like. The term "optionally substituted" as used herein means the group to which this term refers may be unsubstituted, or substituted with one or more groups independently selected from halogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocycloalkyl, WO 2004/069801 PCT/AU2004/000132 16 aryl, heteroaryl, cyano, cyanate, isocyanate, -0-alkyl, -S-alkyl, nitro, amino, -C(O)NH 2 , -C(O)NH(alkyl), -C(O)N(alkyl)2, and -C(O)-alkyl, -C(O)-alkenyl, -C(O)-O-alkyl. The language "therapeutically effective amount" is intended to include within its meaning a non-toxic but sufficient amount of a compound or composition of the invention 5 to provide the desired therapeutic effect. The exact therapeutically effective amount of the compound or composition will vary according to factors such as the type of disease of the animal, the age, sex, and weight of the animal, mode of administration, and the ability of the compound or composition to permeate cell membranes and chelate metal ions, such as iron, in cells of the animal. Dosage regima can be adjusted to provide the optimum 10 therapeutic response. For example, several divided doses can be administered daily or the dose can be proportionally reduced as indicated by the exigencies of the therapeutic situation. As used herein the term "treatment", refers to any and all uses which remedy a disease state or symptoms, prevent the establishment of disease, or otherwise prevent, 15 hinder, retard, or reverse the progression of disease or other undesirable symptoms in any way whatsoever. Abbreviations DFO - desferrioxamine EDTA - ethylenediamine tetraacetic acid 20 MTT - [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium] Tf - transferrin "311" - 2-hydroxy-1-naphthylaldehyde isonicotinoyl hydrazone "31 1m" -2-hydroxy-1-naphthylaldehyde nicotinoyl hydrazone DpT - di-2-pyridylketone 3-thiosemicarbazone 25 Dp2mT - di-2-pyridylketone 2-methyl-3-thiosemicarbazone Dp4mT - di-2-pyridylketone 4-methyl-3-thiosemicarbazone Dp44mT - di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone Dp4eT - di-2-pyridylketone 4-ethyl-3-thiosemicarbazone Dp4aT - di-2-pyridylketone 4-allyl-3-thiosemicarbazone 30 Dp4pT - di-2-pyridylketone 4-phenyl-3-thiosemicarbazone PCBH - 2-pyridylketone benzoyl hydrazone PCBBH - 2-pyridylketone 3-bromobenzoyl hydrazone PCAH -2-pyridylketone 4-aminobenzoyl hydrazone PCHH- 2-pyridylketone 4-hydroxybenzoyl hydrazone WO 2004/069801 PCT/AU2004/000132 17 PCIH - 2-pyridylketone isonicotinoyl hydrazone PCTH - 2-pyridylketone 2-thiophenecarboxaldehyde hydrazone PKIH - di-2-pyridylketone isonicotinoyl hydrazone PKBH - di-2-pyridylketone benzoyl hydrazone 5 PKHH - di-2-pyridylketone 4-hydroxybenzoyl hydrazone PKBBH - di-2-pyridylketone 3-bromobenzoyl hydrazone PKAH - di-2-pyridylketone 4-aminobenzoyl hydrazone PKTH - di-2-pyridylketone 2-thiophenecarboxaldehyde hydrazone PK44pH - di-2-pyridylketone 4,4-diphenylcarboxaldehyde semicarbazone 10 PKoctH - di-2-pyridylketone octanoic hydrazone QCIH - 2-quinolinecarboxaldehyde isonicotinoyl hydrazone QCTH - 2-quinolinecarboxaldehyde 2-thiophenecarboxaldehyde hydrazone QCBH - 2-quinolinecarboxaldehyde benzoyl hydrazone QCBBH - 2-quinolinecarboxaldehyde 3-bromobenzoyl hydrazone 15 QCAH - 2-quinolinecarboxaldehyde 4-aminobenzoyl hydrazone QCHH - 2-quinolinecarboxaldehyde 4-hydroxybenzoyl hydrazone Brief description of the drawings Figure 1. (A) General compound structure showing numbering scheme used. (B) Structures of DFO, 311 and Triapine@; (C). General structures of comparative PCIH and 20 QCIH compounds. Figure 2. (A). Structures of representative DpT analogues, including: DpT, Dp2mT, Dp4mT, Dp44mT, Dp4eT, Dp4aT, and Dp4pT. (B) Structures of representative PKIH analogues, including PKIH, PKTH, PKPH, PKAH, PKHH, PKBH, PK44pH, and PKoctH. 25 Figure 3. Bar graphs of the effect of the chelators on "Fe mobilization from prelabelled SK-N-MC neuroepithelioma cells. (A) Comparison of the PKIH and PCIH analogues; (E) Comparison of the QCIH and PCIH analogues. Results are expressed as the mean ± SD of 3 replicates in a typical experiment of 2 experiments performed. Figure 4. Bar graphs of the effect of the chelators on 59 Fe uptake from 59 Fe 30 transferrin ( 59 Fe-Tf) by SK-N-MC neuroepithelioma cells. (A) Comparison of the PKIH and PCIH analogues; (B) Comparison of the QCIH and PCIH analogues. Results are expressed as the mean ± SD of 3 replicates in a typical experiment of 2 experiments performed.

WO 2004/069801 PCT/AU2004/000132 18 Figure 5. Line graphs of the effect of concentration of the PKIH analogues compared to PCTH on: (A) cellular 59 Fe mobilization and (B) internalized 59 Fe uptake from 59 Fe-transferrin ( 59 Fe-Tf). Results are expressed as the mean ± SD of 3 replicates in a typical experiment of 2 experiments performed. 5 Figure 6. Line graph of the influence of representative PKIH analogues, desferrioxamine (DFO), and 2-hydroxy-1-napthylaldehyde isonicotinoyl hydrazone (311) on the proliferation of SK-N-MC neuroepithelioma cells (N) compared to MRC-5 fibroblasts (F). Each data point represents the mean of 3 separate experiments with duplicate determinations in each experiment. 10 Figure 7. Members of the PKIH series of Fe chelators markedly increase WAF1 and GADD45 mRNA expression in SK-N-MC neuroepithelioma cells. The effects of PKIH analogues are compared to DFO, 311 and some chelators of the PCIH series. (A) (i) Ethidium bromide staining of the agarose gel, (ii) GADD45, (iii) WAF1, and (iv) /actin mRNA levels; (B) Densitometric analysis of the results in (A) normalised to the acting 15 loading control. Total RNA was extracted from cells after a 20 h incubation at 37'C with medium alone (control) or medium containing DFO (100 ptM) or the other chelators (25 pM). Northern blotting was performed using standard procedures (see Example 6). The result illustrated is a typical experiment from three experiments performed. Figure 8. PKIH analogues increase iron-regulatory protein (IRP)-RNA-binding 20 activity in SK-N-MC neuroepithelioma cells. (A) Active IRP-RNA-binding activity; (B) Densitometric analysis of the results in (A). The effect of the PKIH analogues were compared to several chelators that acted as internal controls (ie., DFO, 311, PCBBH, and PCAH) and also the Fe donor, ferric ammonium citrate (FAC). Cells were incubated with DFO (100 riM), FAC (100 g/ml) and the remaining chelators (25 piM) for 20 h at 37*C. 25 The IRP-RNA-binding activity was then assessed by the gel-retardation assay using standard techniques. The result illustrated is a typical experiment from three experiments performed. Figure 9. Crystal structure of PKAH as determined by X-ray crystallography showing 30% thermal ellipsoids and water of solvation. 30 Figure 10. DpT analogues and 311 (internal control) show selective anti proliferative activity against immortal SK-N-MC neuroepithelioma cells (S) compared to mortal MRC-5 fibroblasts (F). Cells were incubated in the presence or absence of the compounds (0-25tM) for 72 h at 37'C. After this incubation period, cellular density was measured using the MTT assay. Each data point represents the mean of 2 replicates in a 35 typical experiment of at least 3-5 experiments performed.

WO 2004/069801 PCT/AU2004/000132 19 Figure 11. The effect of the DpT analogues compared to DFO and 311 on 59Fe mobilization and cellular 59 Fe uptake from 59 Fe-transferrin ( 59 Fe-Tf) in SK-N-MC neuroepithelioma and M109 cells. (A) The effect of chelators on 59 Fe mobilization from prelabeled SK-N-MC neuroepithelioma cells. (B) The effect of the chelators at preventing 5 5Fe uptake from 59 Fe-Tf by SK-N-MC cells. (C) The effect of the chelators on 59Fe mobilization from prelabeled M109 cells as a function of chelator concentration. (D) The effect of the chelators at preventing "Fe uptake from 59 Fe-Tf by M109 cells as a function of chelator concentration. Results are expressed as the mean + SD of 3 replicates in a typical experiment of three performed. 10 Figure 12. (A) Dp44mT and to a greater extent, Triapine*, markedly decreased the growth of a M109 lung carcinoma in mice after a 5 day treatment regimen, and (B) the induction of apoptosis in tumors after injection of (i) vehicle control or (ii) Dp44mT as judged by the TUNEL assay. Figure 13. Effect of Dp44mT on M109 cellular apoptosis or necrosis/late stage 15 apoptosis as a function of (A) chelator concentration and (B) incubation time. (A) M109 cells were incubated with Dp44mT at 0-250 piM for 24 h at 37'C. Cellular apoptosis or necrosis/late stage apoptosis were measured by Annexin V-FITC and PI staining, respectively (see Materials and Methods for details). (B) M109 cells were treated with Dp44mT (1 pM) for various incubation times (0-48 h). Cellular apoptosis and 20 necrosis/late stage apoptosis were measured as described in (A). Data plotted are mean SEM of three separate experiments. Figure 14. Effect of Dp44mT (1 ptM) on the (A,B) protein levels of active caspase 3, -8 and -9, and (C,D) the activity of caspase-3, -8 and -9 in cultured M109 cells in the absence (C) and presence (D) of cell-permeable caspase inhibitors. (A) Caspase-3, -8, 25 and -9 levels after Dp44mT treatment at indicated times as detected by western blotting (upper panel). The anti-p-actin antibody was used to ensure equal protein loading (lower panel). (E) Densitometric analysis of the expression of caspase-3, -8, and -9 as a function of time nonnalized to p-actin (C) Caspase activity induced by Dp44mT (1 IM) at 0-48 h was expressed as a percentage of the 0 h time value. (D) Cell-permeable inhibitors of 30 caspase-3, -8 or -9 at 1 pM prevented activation of these enzymes when incubated with Dp44mT (1 pM) for 48 h. Results are mean ± SEM of three separate experiments. Figure 15. Effect of Dp44mT (1 tM) in cultured M109 cells on: (A) the holo cytochrome c (h-cytc) levels in cytosolic and stromal-mitochondrial membrane (SMM) fractions; (B) mitochondrial protein levels of Bcl-2 and Bax; (C) intracellular ROS 35 production.

WO 2004/069801 PCT/AU2004/000132 20 Figure 16. Only the membrane-permeable chelators 311 and Dp44mT with high anti-proliferative activity and Fe chelation efficacy increase Ndrgl protein expression when examined at 25 pM. MCF-7 cells were incubated for 24 h at 37'C with 25 piM of DFO, 311, pencillamine, dp44mT, dp2mT, dipyridyl, Li or EDTA. The expression of 5 Ndrgl and j-actin protein levels were assessed by Western blotting. Results are a typical experiment from 3 performed. Detailed Description of Preferred Embodiments of the Invention The present invention relates to compounds which are capable of chelating metal ions. In particular, the present invention relates to (thio)semicarbazone and 10 (thio)hydrazone compounds of Formulae 1, 2, 3 and 4: (X) -W' Y N Z' A _N E _N E VAQ \N--: IHN W/ Z R' N~ GG H (X)Y (X Formula 1 Formula 2 (R4)n N _N \ E _N E

HN

N H N (R4)p N \L/ 15 Formula 3 and Formula 4 wherein E, A, Z, Y, X, W, V, Z', Y', X', W', m, n, p, q and G are defined above, including provisos (i) and (ii). The present invention also relates to the use of compounds of Formulae 1, 2, 3 and 4 as defined herein, including or excluding provisos (i) and (ii). Accordingly, the present 20 invention encompasses the use of compounds of Formulae la, 2a, 3a and 4a below: A B NN E R' G Formula la wherein E is O or S; 25 A is WO 2004/069801 PCT/AU2004/000132 21 (x (X')n< ~N wherein Z', Y', X' and W' are independently selected from CR 4 , NR 8 , S and 0, wherein the total number of heteroatoms in A is 1, 2 or 3, m is 0 or 1, 5 B is ' (X)q ~z Y wherein Z, Y, X, W and V are independently selected from CR 4 , NR 8 , S and 0; and wherein the total number of heteroatoms in B is 0, 1, 2, or 3; q is 0 or 1; 10 each R 4 is independently selected from hydrogen, halogen, alkyl, alkenyl, optionally substituted amino, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, -C(0)-alkenyl, -C(0)-0 alkyl, nitro and cyano; each R 8 is independently selected from hydrogen, halogen, alkyl, alkenyl, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, and -C(O)-alkenyl, benzyl, benzylcarbamate; 15 R' is H or a group which does not hinder metal ion chelation; -G is NR 2

R

3 or CR 2

R

3

R

5 , wherein the bond between C and R 5 may be a single bond, double bond, or triple bond; and wherein when the bond between C and R 5 is a double bond, one of R 2 and R 3 is absent, and wherein when the bond between C and R 5 is a triple bond, R 2 and R 3 are both 20 absent;

R

5 is selected from the group consisting of hydrogen, halogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted amino, optionally substituted heteroaromatic, an optionally substituted bicyclic group, and an optionally substituted aromatic group; 25 R 2 and R 3 may be the same or different and are individually selected from hydrogen, halogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted amino, optionally substituted heteroaromatic, an optionally substituted bicyclic group, and an optionally substituted aromatic group; or -G is an optionally substituted heteroaromatic group, an optionally substituted 30 bicyclic group, an optionally substituted aromatic group, an optionally substituted alkyl WO 2004/069801 PCT/AU2004/000132 22 group; an optionally substituted cycloalkyl group, or an optionally substituted heterocycloalkyl group; A -N E B HN G Formula 2a s wherein A is selected from: N NON KN N N (R 4)n - (R 4)n - , (R 4)n; a n; I; ; and H wherein each R 4 is independently selected from halogen, alkyl, alkenyl, optionally substituted amino, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, -C(O)-alkenyl, -C(O)-O 10 alkyl, nitro and cyano, nis 0, 1, 2,3 or4; and wherein B, E and G are as defined for Formula 1 a; \ N -N\ E

HN

(4)pN G 15 Formula 3a wherein E is O or S; n is 0, 1, 2, 3 or 4, p is 0, 1, 2, 3 or 4, and 20 each R 4 is independently selected from halogen, alkyl, alkenyl, optionally substituted amino, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, -C(O)-alkenyl, -C(O)-O alkyl, nitro and cyano, and -G is as defined above for Formula la; and -HN-4 \/N G 25 WO 2004/069801 PCT/AU2004/000132 23 Formula 4a wherein E is O or S; and -G is as defined above for Formula 1 a. 5 Accordingly, the expressions "a compound of formula 1, 2, 3 and/or 4 with or without provisos (i) and (ii)", and "a compound of formula 1, 2, 3 and/or 4 including or excluding provisos (i) and (ii)" should be understood to encompass compounds of Formulae 1, 2, 3 and 4, as well as compounds of Formulae la, 2a, 3a and 4a. As used herein, the generic term "(thio)semicarbazone" includes within its scope 10 semicarbazones, thiosemicarbazones, and derivatives or related analogues thereof. Similarly, as used herein, the generic term "(thio)hydrazone" includes within its scope hydrazones, thiohydrazones and derivatives or related analogues thereof. With reference to Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a, when E is 0, the compounds are sometimes referred to herein as "PKIH analogues" or the "PKIH series"; 15 when E is S, the compounds are sometimes referred to herein as "DpT analogues" or the "DpT series". Compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a may chelate metal ions to form metal ion complexes. Examples of metal ions capable of forming a metal ion complex with compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a include transition metal ions, 20 such as for example, iron, copper, zinc, palladium, platinum and gallium ions. In one embodiment the metal ion is iron(III). In another embodiment the metal ion is iron(II). In a further embodiment the metal ion is zinc(II). The invention also encompasses pharmaceutical formulations comprising at least one compound of Formulae 1, 2, 3 and/or 4 including provisos (i) and (ii), and/or a metal 25 ion complex of each said compound. The present invention also relates to therapeutic uses of compounds of Formulae 1, 2, 3 and 4 as defined herein including or excluding provisos (i) and (ii), or compositions comprising such compounds and/or metal ion complexes thereof. Compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a comprise a 30 (thio)semicarbazone or (thio)hydrazone backbone. At least one of groups A and B comprise a heteroatom which is capable of participating in metal ion chelation. In one embodiment, compounds of Formulae 1, 2, 3 and 4 function as tridentate ligands. In one embodiment, at least one of A and B is a 2-pyridyl group, wherein the pyridyl nitrogen, the imino (C=N) nitrogen, and the carbonyl (C=0) or thiocarbonyl (C=S) groups may 35 each serve as donor atoms to coordinate a suitable metal ion. Those skilled in the art will WO 2004/069801 PCT/AU2004/000132 24 appreciate that when A and B are not identical, there may be cis/trans stereoisomerisation about the C=N double bond. Accordingly, in one embodiment, when A and B are non identical optionally substituted heteroaromatic rings, a compound of Formula 1 may be capable of forming a metal ion complex through the heteroatom of ring A or ring B, 5 depending on the cis/trans stereochemistry. This is illustrated schematically below, where M is a suitable metal ion: Et E t / E A G N NH 2 N B N G N ' BI |~ N. ,

H

2 N cis trans Compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a may function as tridentate 10 ligands and two compounds may form a hexacoordinate metal ion complex with a suitable metal ion, e.g., a metal ion capable of forming octahedral complexes. Suitable methods for the synthesis of compounds of Formulae 1, 2, 3 and 4 are well known to those skilled in the art and some are described, for example, in J. March, Advanced Organic Chemistry, 4 th Edition (John Wiley & Sons, New York, 1992); and 15 Vogel's Textbook of Practical Organic Chemistry, 5 th Edition (John Wiley & Sons, New York, 1989). Compounds of Formulae 1, 2, 3 and 4 according to the present invention may be prepared by means of a Schiff base condensation reaction in which a ketone or aldehyde is condensed with either an acid hydrazide or acid thiosemicarbazide of choice to produce 20 the corresponding (thio)semicarbazone or (thio)hydrazone compound of Formula 1, 2, 3 or 4 having a desired substitution pattern. A suitable synthetic method of (thio)semicarbazone and (thio)hydrazone compounds has been described, for example, by Bacchi et al. (1996). A typical example of a general synthetic route for preparing PKIH analogues of Formulae 1, 2, 3 and 4 is 25 represented in the following reaction scheme: (X) -W' ( '

NH

2 Schiff Base Y'9 z I condensation Z 0+ HN G >N 0 0 V/ N (x) (X 4G WO 2004/069801 PCT/AU2004/000132 25 An example of a synthetic route for preparing PKIH analogues of Formula 4, is illustrated below for the compounds PK44pH and PKoctH: N HN Schiff base N N H 2 N 2 i condensation 0+ HN N NN N o ~ PK44pH 5 N H 2 N Schiff base N condensation 0 + NN 0 N O N HN o PKoctH A suitable method for preparing DpT analogues has been described by Johnson et al. (1982). An example of a general synthetic route for preparing DpT analogues of 10 Formulae 1, 2, 3 and 4 as defined herein, is represented in the following general reaction scheme: (X) -W () YI/ N NH 2 Schiff Base Y z' H condensation Z VV HN An example of a synthetic route for preparing DpT analogues of Formula 4 as 15 defined herein, is illustrated below for the compounds Dp4eT and Dp4aT: Schiff base N

NH

2 H condensation + HN N H CN S N HN N S Dp4eT N NH Schiff base N

IH

2 H condensation 0 + HN N H ,N , N HN N S Dp4aT WO 2004/069801 PCT/AU2004/000132 26 The condensation reactions represented above may be carried out under conditions known to those skilled in the art. For example, suitable solvent systems include ethanol, methanol, ethanol/water, methanol/water, or other common organic solvents such as 5 acetone, benzene, toluene, etc. Compounds of Formulae 1, 2, 3 and 4 may be purified using standard techniques, including recrystallisation from a suitable solvent, and column chromatography. In an alternative synthesis, thiosemicarbazones and thiohydrazones may be prepared from the corresponding semicarbazone and hydrazone compounds, respectfully, using 10 methods known to those skilled in the art. For example, a C=O group may be converted into a C=S group using Lawesson's reagent under standard conditions. For example, a semicarbazone or hydrazone compound of Formula 1, 2, 3 or 4 may be heated at reflux with Lawesson's reagent in a suitable solvent, such as toluene or benzene, to produce the corresponding thio- compound. Preparation of compounds of Formula 4 are illustrated in 15 the following general scheme: N Lawesson's N N Reagent, A N N HN G N HN G 0 S Those skilled in the art will understand that protecting groups can be employed as necessary or appropriate in the course of synthesising compounds in accordance with the invention. Suitable protecting groups are known to those skilled in the art. For example, 20 common N-protecting groups include benzyl and carbamates, such as tert-butylcarbamate, benzylcarbamate. Protecting groups are described in Protective Groups in Organic Synthesis, Theodora W. Greene & Peter G. M. Wuts, Second Ed., John Wiley & Sons, Inc. (1991), the contents of which are incorporated herein by reference. Examples of (thio)semicarbazone and (thio)hydrazone derivatives suitable for use 25 in accordance with the present invention include the following: di-2-pyridylketone isonicotinoyl hydrazone ("PKIH"); di-2-pyridylketone benzoyl hydrazone ("PKBH"); di 2-pyridylketone 4-hydroxybenzoyl hydrazone ("PKHH"); di-2-pyridylletone 3 bromobenzoyl hydrazone ("PBBH"); di-2-pyridylketone 4-aminobenzoyl hydrazone ("PKAH"); di-2-pyridylketone 2-thiophenecarboxaldehyde hydrazone ("PKTH"); di-2 30 pyridylketone 4,4-diphenylcarboxaldehyde semicarbazone ("PK44pH"); di-2 pyridylketone octanoic hydrazone ("PKoctH"); di-2-pyridylketone 4-methyl-3- WO 2004/069801 PCT/AU2004/000132 27 thiosemicarbazone ("Dp4mT"); di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone ("Dp44mT"); di-2-pyridylketone 4-ethyl-3-thiosemicarbazone ("Dp4eT"); di-2 pyridylketone 4-allyl-3-thiosemicarbazone ("Dp4aT"); di-2-pyridylketone 4-phenyl-3 thiosemicarbazone ("Dp4pT"). 5 Also disclosed herein are thiohydrazone analogues which correspond structurally to the PKIH analogues, but have a C=S instead of C=O. Such compounds include di-2 pyridylketone isonicotinoyl thiohydrazone ("PKITH"); di-2-pyridylketone benzoyl thiohydrazone ("PKBTH"); di-2-pyridylketone 4-hydroxybenzoyl thiohydrazone ("PKHTH"); di-2-pyridylketone 3-bromobenzoyl thiohydrazone ("PBBTH"); di-2 10 pyridylketone 4-aminobenzoyl thiohydrazone ("PKATH"); di-2-pyridylketone 2 thiophenecarboxaldehyde thiohydrazone ("PKTTH"); di-2-pyridylketone 4,4 diphenylcarboxaldehyde thiosemicarbazone ("PK44pTH"); di-2-pyridylketone octanoic thiohydrazone ("PKoctTH"). Iron complexes in accordance with the present invention include Fe[PKIH] 2 , 15 Fe[PKBH] 2 , Fe[PKHH] 2 , Fe[PBBH] 2 , Fe[PKAH] 2 , Fe[PKTH] 2 , Fe[PK44pH] 2 and Fe[PKoctH] 2 , Fe[Dp4mT] 2 , Fe[Dp44mT] 2 , Fe[Dp4eT] 2 , Fe[Dp4aT] 2 , and Fe[Dp4pT] 2 . The iron ion may be Fe(II) or Fe(III). Metal ion complexes of PKIH and DpT analogues of Formulae 1, 2, 3 and 4 in accordance with the present invention may be readily prepared using techniques and 20 reagents well known to those skilled in the art. For example, suitable metal ion salts include, but are not limited to, metal halides, nitrates, sulfates, perchlorates, acetates, and triflates. Suitable iron salts for forming iron complexes include, but are not limited to, FeCl 3 , Fe(NO3) 3 , FeSO 4 , Fe(OAc) 3 , and Fe 2 (Cl0 4 )3.

3 In the definition of Formula 1, the group R' is defined as "hydrogen or a group that 25 does not prevent the compound chelating a metal ion". When R' is methyl, Fe chelation is hindered. The presence of two organic groups (i.e, aromatic and/or heteroaromatic groups A and B) at the 1-position of compounds of Formulae 1, 2, 3 and 4 generally increases the lipophilicity of the compounds in comparison to compounds without two such groups at 30 that position. PKIH and DpT analogues of Formulae 1, 2, 3 and 4 may be capable of permeating cell membranes. Structure-activity relationships of the PCIH, QCIH (Figure IC), PKIH and DpT series of compounds, indicate that even though these classes of compound appear to have a similar metal-binding site, the lipophilicity, efficacy of metal ion chelation, and 35 antiproliferative properties of the respective classes may differ substantially.

WO 2004/069801 PCT/AU2004/000132 28 (Thio)semicarbazone and (thio)hydrazone compounds of Formulae 1, 2, 3 and 4 in accordance with the present invention may function as tridentate ligands capable of forming metal ion complexes. A general example of an iron complex is represented below for a compound of Formula 1. w-(Xg V / r Y' '\zr N=N E !-,FeAME G , Z' S1.N' 'Y' I IV -W Z w ' 5 ) ,where E is O or S. As a further example, an iron complex of the ligand PKAH, is illustrated below. -- N /\r N NN O F e-O \ / NH 2 N

H

2 N I N ~

N-

"Fe[PKAH]2" Also for example, an iron complex of the ligand Dp4mT, is illustrated below. -- N - I I CH 3 H3CFe:S H A N H NNN

N-

10 '"Fe[Dp4mT] 2 " In accordance with the present invention, metal ion complexes of DpT or PKIH ligands may be neutral or charged.

WO 2004/069801 PCT/AU2004/000132 29 Also disclosed herein is a process for identifying an anti-proliferative compound, wherein the process comprises exposing (eg, contacting, or incubating) a cell or cellular extract having a gene encoding one or more cell cycle inhibitors, with a compound capable of chelating iron, determining the level of expression of the gene, determining s whether the level of expression of the gene differs in comparison to the expression of the gene in the absence of said compound; and thereby determining whether said compound has anti-proliferative activity. A similar process may be used for assessing a plurality of compounds to identify one or more anti-proliferative compounds, the process comprising exposing (eg, 10 contacting, incubating) a cell or cellular extract having gene encoding one or more cell cycle inhibitors, with a plurality of compounds capable of chelating iron, determining whether any of said compounds modify the level of expression of said gene, and if so, separately determining the level of expression of said gene for each compound, determining whether said level of expression differs in comparison to the level of 15 expression of said gene in the absence of each compound; and thereby determining whether each compound has anti-proliferative activity. The gene may be sensitive to cellular iron concentration. Expression of the gene may be up-regulated or down-regulated in response to Fe depletion. Examples of genes include WAFi, GADD45, and Ndrgl. Expression of the gene (e.g., mRNA, protein) in the 20 absence of a compound being assessed may be separately determined prior to, or after, detennining expression of the gene in the presence of the compound. Modification of Cellular Function Compounds which are capable of chelating cellular iron may modify cellular 25 function, eg, through enzyme inhibition, modification of gene expression (including upregulation and downregulation of genes), inhibition of translation (eg, of proteins, including p21), generation of Reactive Oxygen Species (ROS), etc, and may be therapeutically useful for inhibiting cellular proliferation and/or inducing apoptosis. For example, depletion of cellular iron may modify the activity of iron-dependent enzymes, 30 such as ribonucleotide reductase. Iron deprivation can also induce Gi-S cell arrest and apoptosis. The genes WAFI and GADD45 encode cell cycle inhibitors essential for Gi-S arrest (including cyclin dependent kinase inhibitors such as p 2 1CIP1/WAFI)30 Upregulation of these genes, e.g., through iron depletion, may induce cell cycle arrest. The gene Ndrg] 35 (N-myc down-regulated gene 1) is sensitive to cellular iron concentration.

WO 2004/069801 PCT/AU2004/000132 30 Overexpression of Ndrg1, eg., in response to cellular iron deprivation may result in Gi-S cellular arrest. Overexpression of Ndrgl has also been shown to slow tumour growth and inhibit tumour cell metastasis.

4

-

7 Compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a according to the present 5 invention are capable of chelating cellular metal ions, including iron ions. Accordingly, compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a may be capable of inhibiting DNA synthesis by inhibition of ribonucleotide reductase. Ribonucleotide reductase is the rate limiting step in DNA synthesis and is expressed in higher levels in neoplastic cells in comparison to normal cells. Compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a may 10 also result in overexpression of Fe-sensitive genes, such as WAF1, GADD45 and Ndrg1, and therefore may be useful for inhibiting cellular proliferation. Overexpression of Fe sensitive genes such as WAF1, GADD45 and Ndrg] may occur at the mRNA level. Monitoring expression of WAF1, GADD45 and Ndrg] may provide a useful indicator of the anti-proliferative potential of compounds which chelate metal ions, including Fe. 15 Apoptosis, or programmed cell death, plays an integral role in cellular turnover. Apoptosis can be induced in response to cellular iron depletion. Caspase enzymes are common executors of apoptosis.8,9 Two caspase-activating cascades that regulate apoptosis have been described, one of which is initiated via death receptors (eg., CD95), whereas the other is triggered by changes in mitochondrial integrity.' 1 0 In the former, 20 engagement of death receptors by their ligands (eg., CD95L) leads to the formation of a death-inducing signalling complex.

8 Activated death receptors recruit and activate caspase-8 which initiates "executioner" caspases, including caspase-3. In mitochondrial dependent apoptosis, release of holo-cytochrome c (h-cytc) into the cytosol results in the formation of an "apoptosome" containing h-cytc, Apaf-1 and pro-caspase-9.8'9 In the 25 apoptosome, pro-caspase-9 becomes activated and triggers executioner caspases that initiate terminal events overlapping with those induced by the death receptor pathway.' 9 Major modulators of the mitochondrial pathway of apoptosis include the Bcl-2/Bax gene family.

8

,

11

,

1 2 The Bcl-2 group of proteins are found in the outer mitochondrial membrane and play a role in its stability.

8

'

1 ' In contrast, the Bax proteins are cytosolic molecules that 30 upon receipt of an apoptotic signal migrate to the mitochondrion where they bind to the permeability transition pore.8, 12 Imbalance of Bcl-2 and Bax induces the loss of selective ion permeability resulting in h-cytc release that initiates the caspase cascade responsible for apoptosis.8,12 Chelation of cellular metal ions, such as iron and.possibly zinc by compounds of Formulae 1, 2, 3 and 4 may initiate the caspase cascade and induce 35 apoptosis.

WO 2004/069801 PCT/AU2004/000132 31 Cellular metal ion depletion, including depletion of cellular iron, may lead to apoptosis. Apoptosis may also be induced in response to oxidative stress which can lead to release of h-cytc from the mitochondrion and an imbalance of Bcl-2/Bax expression. Metal ion complexes, including Fe-complexes of compounds of Formulae 1, la, 2, 2a, 3, 5 3a, 4 and 4a may be capable of inducing apoptosis through the generation of reactive oxygen species. Accordingly, compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a, as well as metal ion complexes of compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a (including iron complexes), may be therapeutically useful, e.g., as anti-tumour agents. Treatment and/or Prevention of Disease 10 DpT and PKIH compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a disclosed herein may be useful in the treatment of various disorders and diseases of vertebrates. Examples of disorders and diseases may be grouped into broad categories, including the following: angiogenesis-dependent diseases, cellular proliferative diseases (e.g. psoriasis, IBD, malignancies, restenosis), cancer. 15 The cancer may be selected from the group including, but not limited to, carcinogenic tumours, tumours of epithelial origin, such as colo-rectal cancer, breast cancer, lung cancer, head and neck tumours, hepatic cancer, pancreatic cancer, ovarian cancer, gastric cancer, brain cancer, bladder cancer, prostate cancer and urinary/genital tract cancer; mesenchymal tumours, such as sarcoma; and haemopoietic tumours such as 20 B cell lymphoma. For example, the cancer may be a haematological tumour, a solid tumour, a non-solid tumour, (e.g., leukaemia, lymphoma). DpT and PKIH analogues of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a disclosed herein may be used in combination with other known treatments, such as surgery and/or therapeutic agents, including chemotherapeutic or radiotherapeutics. For example, when 25 used in the treatment of solid tumours, compounds of the present invention may be administered with chemotherapeutic agents such as: adriamycin, taxol, fluorouricil, melphalan, cisplatin, alpha interferon, COMP (cyclophosphamide, vincristine, methotrexate and prednisone), etoposide, mBACOD (methotrexate, bleomycin, doxorubicin, cyclophosphamide, vincristine and dexamethasone), PROMACE/MOPP 30 (prednisone, methotrexate (w/leucovin rescue), doxorubicin, cyclophosphamide, taxol, etoposide/mechlorethamine, vincristine, prednisone and procarbazine), vincristine, vinblastine, angioinhibins, TNP-470, pentosan polysulfate, platelet factor 4, angiostatin, LM-609, SU-101, CM-101, Techgalan, thalidomide, SP-PG and the like. Other chemotherapeutic agents include alkylating agents such as nitrogen mustards including WO 2004/069801 PCT/AU2004/000132 32 mechloethamine, melphan, chlorambucil, cyclophosphamide and ifosfamide; nitrosoureas including carmustine, lomustine, semustine and streptozocin; alkyl sulfonates including busulfan; triazines including dacarbazine; ethyenimines including thiotepa and hexamethylmelamine; folic acid analogues including methotrexate; pyrimidine analogues 5 including 5-fluorouracil, cytosine arabinoside; purine analogues including 6 mercaptopurine and 6-thioguanine; antitumour antibiotics including actinomycin D; the anthracyclines including doxorubicin, bleomycin, mitomycin C and methramycin; hormones and hormone antagonists including tamoxifen and cortiosteroids and miscellaneous agents including cisplatin and brequinar. 10 The physiological system to be treated in accordance with the present invention (e.g, the hepatic system, pancreatic system) may be isolated by or during surgery prior to administration of one or more compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a. Pharmaceutical and/or Therapeutic Formulations In accordance with the present invention, when used for the treatment of disease, 15 compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a may be administered alone. Alternatively, the compounds may be administered as a pharmaceutical formulation which comprises at least one compound of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a, and/or a metal ion complex thereof. Compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a may also be present as pharmaceutically acceptable salts. 20 By pharmaceutically acceptable salt it is meant those salts which, within the scope of sound medical judgement, are suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. 25 For instance, suitable pharmaceutically acceptable salts of PKIH and DpT analogues in accordance with the present invention may be prepared by mixing a pharmaceutically acceptable acid such as hydrochloric acid, sulfuric acid, methanesulfonic acid, succinic acid, fumaric acid, maleic acid, benzoic acid, phosphoric acid, acetic acid, oxalic acid, carbonic acid, tartaric acid, or citric acid with the 30 compounds of the invention. Suitable pharmaceutically acceptable salts of the compounds of the present invention therefore include acid addition salts. For example, S. M. Berge et al. describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66:1-19. The salts can be prepared in situ during the final isolation and purification of the compounds of the invention, or separately by WO 2004/069801 PCT/AU2004/000132 33 reacting the free base function with a suitable organic acid. Representative acid addition salts include acetate, adipate, alginate, ascorbate, asparate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fumarate, 5 glucoheptonate, glycerophosphate, hemisulfate, heptonate, hexanoate, hydrobromide, hydrochloride, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3 phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, 10 tartrate, thiocyanate, toluenesulfonate, undecanoate, valerate salts, and the like. Representative alkali or alkaline earth metal salts include sodium, lithium potassium, calcium, magnesium, and the like, as well as nontoxic ammonium, quaternary ammonium, and amine cations, including, but not limited to ammonium, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, 15 trimethylamine, triethylamine, ethylamine, and the like. Convenient modes of administration include injection (subcutaneous, intravenous, etc.), oral administration, inhalation, transdennal application, or rectal administration. Depending on the route of administration, the analogue may be coated with a material to protect the analogue from the action of enzymes, acids and other natural conditions which 20 may inactivate the therapeutic activity of the analogue. The compound may also be administered parenterally or intraperitoneally. Dispersions of compounds of Formulae 1, la, 2, 2a, 3, 3a, 4 and 4a may also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof and in oils. Under ordinary conditions of storage and use, pharmaceutical preparations may contain a 25 preservative to prevent the growth of microorganisms. Pharmaceutical compositions suitable for injection include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. Ideally, the composition is stable under the conditions of manufacture and storage and may include a preservative to 30 stabilise the composition against the contaminating action of microorganisms such as bacteria and fungi. In a preferred embodiment, the compound is administered orally, for example, with an inert diluent or an assimilable edible carrier. The compound and other ingredients can also be enclosed in a hard or soft shell gelatin capsule, compressed into tablets, or 35 incorporated directly into an individual's diet. For oral therapeutic administration, the WO 2004/069801 PCT/AU2004/000132 34 analogue can be incorporated with excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like. Suitably, such compositions and preparations may contain at least 1% by weight of active compound. The percentage of the PKIH or DpT analogue of Formulae 1, la, 2, 2a, 3, 3a, 5 4 and 4a in pharmaceutical compositions and preparations can, of course, be varied and, for example, can conveniently range from about 2% to about 90%, about 5% to about 80%, about 10% to about 75%, about 15% to about 65%; about 20% to about 60%, about 25% to about 50%, about 30% to about 45%, or about 35% to about 45%, of the weight of the dosage unit. The amount of analogue in therapeutically useful compositions is such 10 that a suitable dosage will be obtained. The language "pharmaceutically acceptable carrier" is intended to include solvents, dispersion media, coatings, anti-bacterial and anti-fungal agents, isotonic and absorption delaying agents, and the like. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or is agent is incompatible with the analogue, use thereof in the therapeutic compositions and methods of treatment is contemplated. Supplementary active compounds can also be incorporated into the compositions according to the present invention. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to 20 physically discrete units suited as unitary dosages for the individual to be treated; each unit containing a predetermined quantity of analogue is calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the novel dosage unit forms of the invention are dictated by and directly dependent on (a) the unique characteristics of the analogue and the particular therapeutic 25 effect to be achieved, and (b) the limitations inherent in the art of compounding such an analogue for the treatment of proliferative diseases, iron-related conditions, or iron overload disorders in individuals. The principal analogue is compounded for convenient and effective administration in effective amounts with a suitable pharmaceutically acceptable carrier in an acceptable dosage unit. In the case of compositions containing 30 supplementary active ingredients, the dosages are determined by reference to the usual dose and manner of administration of the said ingredients. In one embodiment, the carrier is an orally administrable carrier. In another embodiment, the carrier is suitable for intravenous administration. One suitable form of a pharmaceutical composition is a dosage form formulated as 35 enterically coated granules, tablets or capsules suitable for oral administration.

WO 2004/069801 PCT/AU2004/000132 35 In the case of injectable solutions, the carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyetheylene glycol, and the like), suitable mixtures thereof, and vegetable oils. The proper fluidity can be maintained, for example, by the use of a coating such as 5 lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by including various anti-bacterial and/or anti-fungal agents. Suitable agents are well known to those skilled in the art and include, for example, parabens, chlorobutanol, phenol, benzyl alcohol, ascorbic acid, thimerosal, and the like. In many cases, it may be 10 preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminium monostearate and gelatin. Sterile injectable solutions can be prepared by incorporating the analogue in the is required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilisation. Generally, dispersions are prepared by incorporating the analogue into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above. Tablets, troches, pills, capsules and the like can also contain the following: a binder 20 such as gum gragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose or saccharin or a flavouring agent such as peppermint, oil of wintergreen, or cherry flavouring. When the dosage unit form is a capsule, it can contain, in addition to 25 materials of the above type, a liquid carrier. Various other materials can be present as coatings or to otherwise modify the physical form of the dosage unit. For instance, tablets, pills, or capsules can be coated with shellac, sugar or both. A syrup or elixir can contain the analogue, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and flavouring such as cherry or orange flavour. Of course, any 30 material used in preparing any dosage unit form should be pharmaceutically pure and substantially non-toxic in the amounts employed. In addition, compounds of Formulae 1, 2, 3 and 4 may be incorporated into sustained-release preparations and formulations. A pharmaceutical composition may further include a suitable buffer to minimise acid hydrolysis. Suitable buffer agent agents are well known to those skilled in the art and 35 include, but are not limited to, phosphates, citrates, carbonates and mixtures thereof.

WO 2004/069801 PCT/AU2004/000132 36 Single or multiple administrations of a pharmaceutical compositions according to the invention may be carried out. One skilled in the art would be able, by routine experimentation, to determine effective, non-toxic dosage levels of the compound and/or composition of the invention and an administration pattern which would be suitable for 5 treating the disorders or diseases to which the compounds and compositions are applicable. Further, it will be apparent to one of ordinary skill in the art that the optimal course of treatment, such as the number of doses of the compound or composition of the invention given per day for a defined number of days, can be ascertained using 10 conventional course of treatment determination tests. Generally, an effective dosage per 24 hours may be in the range of about 0.0001 mg to about 1000 mg per kg body weight; suitably, about 0.001 mg to about 750 mg per kg body weight; about 0.01 mg to about 500 mg per kg body weight; about 0.1 mg to about 500 mg per kg body weight; about 0.1 mg to about 250 mg per kg body weight; or about is 1.0 mg to about 250 mg per kg body weight. Still suitably, an effective dosage per 24 hours may be in the range of about 1.0 mg to about 200 mg per kg body weight; about 1.0 mg to about 100 mg per kg body weight; about 1.0 mg to about 50 mg per kg body weight; about 1.0 mg to about 25 mg per kg body weight; about 5.0 mg to about 50 mg per kg body weight; about 5.0 mg to about 20 mg per kg body weight; or about 5.0 mg to 20 about 15 mg per kg body weight. Alternatively, an effective dosage may be up to about 500 mg/m 2 . Generally, an effective dosage may be in the range of about 25 to about 500 mg/m 2 , about 25 to about 350 mg/m 2 , about 25 to about 300 mg/m 2 , about 25 to about 250 mg/m 2 , about 50 to about 250 mg/m 2 , or about 75 to about 150 mg/m 2 . 25 By way of example, suitable dosage forms in accordance with the present invention include the following: Tablet Compound of Formula 1, 2, 3 and 4 0.01 to 20 mg, generally 0.1 to 10 mg Starch 10 to 20 mg 30 Lactose 100 to 250 mg Gelatin 0 to 5 mg Magnesium Stearate 0 to 5 mg Injectable Solution Compound of Formula 1, 2, 3 and 4 0.01 to 20 mg, generally 0.1 to 10 mg WO 2004/069801 PCT/AU2004/000132 37 Sodium Chloride 8.5 mg Potassium Chloride 3 mg Calcium Chloride 4.8 mg Water for injection, q.s. to 10 ml 5 It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be made to the invention as shown in the specific embodiments without departing from the spirit or scope of the invention as broadly described. The present embodiments are, therefore, to be considered in all respects as illustrative and not 10 restrictive. The invention will now be described in greater detail by reference to specific examples, which should not be construed as in any way limiting the scope of the invention. EXAMPLE 1 - Synthesis of Compounds 15 Comparative 2-pyridyl- and quinolyl-aroylhydrazone analogues, referred to herein as "PCIH" and "QCIH" analogues, were synthesised, respectively, by Schiff base condensation of 2-pyridylcarboxaldehyde or 2-quinolinecarboxaldehyde with an appropriate acid hydrazide. The comparative compounds pyridoxal isonicotinoyl hydrazone (PIH) and 2 20 hydroxy-1-naphthylaldehyde isonicotinoyl hydrazone (311), were synthesised by the method of Richardson & Bernhardt (1999).' Representative structures of the PCIH, QCIH aroylhydrazone series of compounds and "311" are provided in Figure 1B and 1C. Example la - Synthesis of PKIH Analogues 25 Representative PKIH analogues were synthesised by condensing 2-di-pyridyl ketone with an appropriate acid hydrazide according to the method of Bacchi et al., (1996)." Suitable solvents include ethanol, methanol, ethanol/water, methanol/water, acetone, benzene, toluene. Structures of representative PKIH analogues are shown in Figure 2B. 30 An X-ray crystal structure of PKAH is provided in Figure 9. The X-ray crystallographic data is provided below. Crystal data collection: WO 2004/069801 PCT/AU2004/000132 38 CAD-4 software (Enraf-Nonius, 1989); cell refinement: SET4 in CAD-4 software; data reduction: Xtal (Hall et al., 1992); programs used to solve structure: SHELX86 (Sheldrick, 1990); Program used to refine structure: SHELXL97 (Sheldrick, 1997); molecular graphic: PLA TON (Spek, 1990; software used to generate crystallographic 5 data: SHELXL 97. Data collection was obtained under same general conditions as described by D. R. Richardson, E. Becker and P. V. Bernhardt. (1999). Crystal Data and Details of the Structure Determination for: PKAH (C18 H16 N5 0) P-1 (2) Z = 2 10 Crystal Data Empirical Formula: C18 H16 N5 O, O Formula Weight 334.36 Crystal System Triclinic Space group P-1 (No. 2) 15 a, b, c [Angstrom] 8.667(2) 9.977(2) 11.197(2) alpha, beta, gamma [deg] 91.010(10) 109.20(2) 109.63(2) V [Ang**3] 852.2(3) Z 2 D(calc) [g/cm**3] 1.303 20 F(000) , 350 Mu(MoKa) [ /mm ] 0.1 Final Structure Factor Calculation for PKAH (C18 H16 N5 0) P-1 (2) Z 2 Total number of 1.s. parameters = 240 Maximum vector length = 511 Memory required 2380 / 22995 25 wR2 = 0.1767 before cycle 21 for 2985 data and 0 / 240 parameters GooF = S = 0.987; Restrained GooF = 0.987 for 0 restraints Weight = 1 / [ sigma^2(Fo^2) + (0.1000 * P )A2 + 0.00 * P ] where P = (Max ( Fo^2, 0 )+ 2 * FcA2 ) / 3 Ri = 0.0503 for 1497 Fo > 4sig(Fo) and 0.1265 for all 2985 data 30 wR2 = 0.1767, GooF = S = 0.987, Restrained GooF = 0.987 for all data Occupancy sum of asymmetric unit =25.00 for non-hydrogen and 16.00 for hydrogen atoms Bond lengths and angles Cl - Distance Angles Ni 1.3378 (0.0036) 35 C2. 1.3775 (0.0042) 122.49 (0.27) C6 1.4967 (0.0038) 116.20 (0.26) 121.26 (0.27) Ci - Ni C2 C2 - Distance Angles 40 C3 1.3738 (0.0042) Cl 1.3775 (0.0041) 119.20 (0.31) C2 - C3 C3 - Distance Angles 45 C4 1.3623 (0.0049) C2 1.3738 (0.0042) 118.91 (0.33) C3 - C4 C4 - Distance Angles 50 C3 1.3623 (0.0049) C5 1.3668 (0.0047) 118.81 (0.31) C4 - C3 WO 2004/069801 PCT/AU2004/000132 39 C5 - Distance Angles Ni 1.3420 (0.0038) C4 1.3668 (0.0047) 123.65 (0.32) 5 C5- NI C6 - Distance Angles N3 1.2972 (0.0033) C7 1.4777 (0.0039) 128.71 (0.25) 10 Cl 1.4967 (0.0038) 111.31 (0.24) 119.98 (0.24) C6- N3 C7 C7 - Distance Angles N2 1.3265 (0.0037) 15 C8 1.3871 (0.0040) 121.37 (0.28) C6 1.4777 (0.0039) 117.66 (0.25) 120.96 (0.27) C7- N2 C8 C8 - Distance Angles 20 C9 1.3698 (0.0044) C7 1.3871 (0.0040) 119.81 (0.31) C8 - C9 C9 - Distance Angles 25 C10 1.3489 (0.0050) C8 1.3698 (0.0044) 119.27 (0.33) C9 - C1O C10 - Distance Angles 30 C9 1.3489 (0.0050) Cl 1.3641 (0.0052) 117.97 (0.35) C10 - C9 Cl - Distance Angles 35 N2 1.3423 (0.0041) C10 1.3641 (0.0052) 124.54 (0.36) C1l - N2 C12 - Distance Angles 40 01 1.2202 (0.0032) N4 1.3690 (0.0034) 120.54 (0.27) C13 1.4649 (0.0039) 122.80 (0.26) 116.56 (0.25) C12 - 01 N4 45 C13 - Distance Angles C14 1.3913 (0.0038) C18 1.3927 (0.0038) 116.65 (0.26) C12 1.4649 (0.0039) 124.91 (0.25) 118.42 (0.26) C13- C14 C18 50 C14 - Distance Angles C15 1.3794 (0.0039) C13 1.3913 (0.0038) 121.52 (0.26) C14 - C15 55 C15 - Distance Angles C14 1.3794 (0.0039) C16 1:3878 (0.0040) 120.80 (0.28) C15 - C14 60 WO 2004/069801 PCT/AU2004/000132 40 C16 - Distance Angles N5 1.3677 (0.0035) C15 1.3878 (0.0040) 121.49 (0.28) C17 1.3939 (0.0040) 120.60 (0.27) 117.90 (0.27) 5 C16 - N5 C15 C17 - Distance Angles C18 1.3592 (0.0040) C16 1.3939 (0.0040) 120.71 (0.27) 10 C17 - C18 C18 - Distance Angles C17 1.3592 (0.0040) C13 1.3927 (0.0038) 122.40 (0.27) 15 C18- C17 Ni - Distance Angles Cl 1.3378 (0.0036) C5 1.3420 (0.0038) 116.87 (0.28) 20 Ni- Cl N2 - Distance Angles C7 1.3265 (0.0037) Cl 1.3423 (0.0042) 117.02 (0.28) 25 N2- C7 N3 - Distance Angles C6 1.2972 (0.0033) N4 1.3639 (0.0030) 119.64 (0.23) 30 N3- C6 N4 - Distance . Angles N3 1.3639 (0.0030) C12 1.3690 (0.0034) 118.10 (0.23) 35 N4 - N3 N5 - Distance Angles C16 1.3677 (0.0035) N5 40 01 - Distance Angles C12 1.2202 (0.0032) 01 45 Final Coordinates and Equivalent Isotropic Displacement Parameters of the non-Hydrogen atoms for: PKAH (C18 H16 N5 0) P-1 (2) Z = 2 Atom x y z U(eq) [Ang^2] 50 01 1.04785 0.75127 0.33347 0.0701 Ni 0.49528 0.32443 -0.06396 0.0535 N2 0.58397 0.30148 0.32830 0.0725 N3 0.80911 0.49927 0.21355 0.0479 N4 0.84496 0.54222 0.33916 0.0501 55 N5 1.03285 0.90464 0.88111 0.0752 Cl 0.65900 0.35466 0.01770 0.0462 C2 0.79735 0.37032 -0.02257 0.0553 C3 0.76880 0.36059 -0.15113 0.0668 C4 0.60365 0.33400 -0.23528 0.0672 60 C5 0.47153 0.31568 -0.18890 0.0628 WO 2004/069801 PCT/AU2004/000132 41 C6 0.68602 0.37702 0.15670 0.0462 C7 0.57670 0.26672 0.21116 0.0511 C8 0.47317 0.13076 0.14286 0.0639 C9 0.37451 0.02995 0.19607 0.0772 5 C10 0.38249 0.06372 0.31568 0.0877 C11 0.48770 0.19900 0.37791 0.0969 C12 0.96466 0.67714 0.39312 0.0484 C13 0.97835 0.72880 0.52083 0.0444 C14 0.88621 0.64856 0.59243 0.0544 10 C15 0.90329 0.70590 0.71113 0.0582 C16 1.01258 0.84689 0.76276 0.0541 C17 1.10477 0.92790 0.69123 0.0615 C18 1.08799 0.86970 0.57489 0.0577 02 0.85377 0.76504 0.05842 0.0756 15 U(eq) = 1/3 of the trace of the orthogonalized U Tensor Example lb - Preparation of DpT Analogues DpT analogues were synthesised by Schiff base condensation of 2-di-pyridylketone and the respective thiosemicarbazides or acid hydrazides using standard procedures 20 (Johnson et al., 1982).14 Suitable solvents for carrying out the reaction include ethanol, methanol, ethanol/water, methanol/water, acetone, benzene, toluene. Structures of representative DpT analogues are shown in Figure 2A. Example 1c - Preparation of Iron Complexes 25 Iron complexes of PKIH analogues were prepared using techniques described by Richardson & Bernhardt, 1999.3 Generally, 1 mole equivalent of an Fe(III) salt was refluxed in hot ethanol with 2 mole equivalents of a PKIH analogue for 1 hour. A dark green/black complex solid formed and was isolated from the mother liquor by filtration under reduced pressure. The complex was then washed with ethanol and air dried. 30 Iron complexes of DpT analogues were prepared in an analogous manner. EKAMPLE 2 - Comparison of Lipophilicity and Activity of PCIH, QCIH and PKH Analogues Lipophilicity was measured as the average log P values (n-octanol-water partition 35 coefficients) for all chelators. Log P values were estimated (Table 1) according to Broto's method (Broto et al., 1984),15 Crippen's fragmentation procedure (Ghose & Crippen, 1987),16 or Viswanadhan's fragmentation procedure (Viswanadhan et al., 1987)17 using Chem Draw Pro. (v. 4.5, Cambridge Software, 1997). The means of these values (average calculated log P values; Table 1) were then plotted against either the IC 50 values, or the 40 ability of chelators to induce 59 Fe mobilisation or prevent 59 Fe uptake. These plots demonstrate no strong correlation between lipophilicity and anti-proliferative activity or WO 2004/069801 PCT/AU2004/000132 42 Fe chelation efficacy of the compounds tested (all correlations: r < 0.52) (data not shown). 5 Table 1: Calculated n-octanol partition coefficients (log Pcac) Crippens' Viswanadhans' Brotos' Method Average Fragmentation Fragmentation log Pcaic PIH 0.43 0.63 -0.16 0.30 311 2.63 2.83 2.17 2.54 PCIH 1.11 1.20 0.56 0.96 PCTH 2.43 2.54 * ** PCBH 2.45 2.51 1.68 2.21 PCBBH 3.28 3.30 2.56 3.05 PCAH 1.65 1.73 0.86 1.41 PCHH 2.06 2.23 1.29 1.86 PKIH 1.66 1.83 0.93 1.47 PKTH 2.98 3.17 * ** PKBH 3.00 3.15 2.05 2.73 PKBBH 3.83 3.94 2.93 3.57 PKAH 2.20 2.36 1.23 1.93 PKHH 2.61 2.86 1.66 2.38 QCIH 2.11 2.20 1.68 2.00 QCTH 3.43 3.54 * ** QCBH 3.45 3.52 2.80 3.26 QCBBH 4.28 4.31 3.68 4.09 QCAH 2.65 2.73 1.98 2.45 QCHH 3.06 3.23 2.41 2.90 * Unable to be calculated by Broto's method ** Unable to calculate Log Pcalc values were calculated using the program Chem Draw Pro V 4.5. 10 The comparative QCIH analogues were the most lipophilic of the compounds studied due to the presence of the single quinoline ring (Table 1). However, the iron chelation efficacy and anti-proliferative activity of the QCIH series is very low (see Examples 3a, 3b and 4a). 15 In Vitro and In Vivo STUDIES General Methodology for Examples 3-10 DFO was purchased from Novartis (Basel, Switzerland).

WO 2004/069801 PCT/AU2004/000132 43 3 311 was prepared according to standard methods. Triapine@ (3-aminopyridine-2-carboxaldehyde thiosemicarbazone; Figure 1B) was a gift from Vion Pharmaceuticals, NJ, USA. Presentation of Compounds 5 All compounds were dissolved in dimethyl sulfoxide (DMSO) as 10 mM stock solutions immediately prior to an experiment and then diluted in 10% fetal calf serum (FCS; Commonwealth Serum Laboratories, Melbourne, Australia) so that the final DMSO concentration was equal to or less than 0.5 % (v/v). Cell Culture Preparation 10 The human SK-N-MC neuroepithelioma cell line, SK-Mel-28 melanoma cells, and MCF-7 breast cancer cells were purchased from the American Type Culture Collection (ATCC), Rockville, MD, USA. The nonnal cell types MRC-5 fibroblasts and L8 rat skeletal muscle cells were also obtained from the American Type Culture Collection (ATCC), Rockville, MD, USA. The cells were grown in Eagle's modified minimum 15 essential medium (MEM; Gibco BRL, Sydney, Australia) containing 10% FCS (Commonwealth Serum Laboratories, Melbourne, Australia), 1% (v/v) non-essential amino acids (Gibco), 2 mM L-glutamine (Sigma Chemical Co., St. Louis, MO, USA), 100 ptg/ml of streptomycin (Gibco), 100 U/ml penicillin (Gibco), and 0.28 gg/ml of fungizone (Squibb Phannaceuticals, Montr6al, Canada). This growth medium is 20 subsequently referred to as "complete medium". Cells were grown in an incubator (Forma Scientific, OH, USA) at 37 0 C in a humidified atmosphere of 5% C0 2 /95% air and subcultured. Cellular growth and viability were carefully assessed using phase-contrast microscopy and trypan blue staining. MTT cellular proliferation assay 25 Cellular proliferation was examined using the MTT (3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium) assay by essentially the same method as described previously.' 8 Cells were seeded in 96-well microtitre plates at 15,000 cells/well in 0.1 mL of MEM medium containing all supplements as described in section 2.2 above (complete medium) and also 1.25 gM human diferric Tf. This seeding density resulted in exponential growth 30 of the cells for the duration of the assay. The cells were grown overnight and the compounds to be tested were then added in 0.1 mL of complete medium containing 1.25 ptM diferric transferrin. The final concentration of the compounds was 0.39-50 pM. Control samples contained complete medium and 1.25 tM diferric Tf. Cells were WO 2004/069801 PCT/AU2004/000132 44 incubated with the compounds for 90 h at 37'C in a humidified atmosphere containing 95% air and 5% CO 2 . After this incubation, 0.01 mL of MTT was added to each well and the plates incubated for 2 h at 37 C. The cells were then solubilised by adding 0.1 mL of 10% SDS-50% isobutanol in 0.01 M HCl and the plates then read at 570 nm on a 5 scanning multiwell spectrophotometer (Titertek Multiscan; Beckman Instruments Inc, California). MTT colour formation was directly proportional to the number of viable cells. The results of the MTT assays are expressed as a percentage of the control value. Statistics Experimental data were compared using Student's paired t-test. Results were 10 considered statistically significant when p < 0.05. Results are presented as the mean or mean ± standard deviation (SD) of 2-5 separate experiments. EXAMPLE 3 - Iron Mobilisation/Uptake Experiments Labelling of Transferrin with 59 Fe 15 Apotransferrin (Sigma Chemical Co., St. Louis, USA) was prepared and labelled with 59 Fe (as ferric chloride in 0.1 M HCl, Dupont NEN, MA, USA) to produce 5 9 Fe transferrin ( 59 Fe-Tf) using standard procedures described by Richardson & Baker, 1992. Example 3a - Comparison of the Effect of PCIH, QCIH and PKIH Analogues on Iron 20 Mobilization from Prelabelled SK-N-MC Neuroepithelioma Cells Methodology: Iron Mobilization Assay The effect of chelators on 59 Fe release from prelabelled cells was studied using standard procedures.

18 Cells were prelabelled with 5 9 Fe-transferrin (0.75 piM) for 3 h at 37'C. The cells were then placed on ice and washed 4 times with ice-cold BSS, and then 25 reincubated for 3 h at 37'C in the presence of medium alone (control) or medium containing DFO (100 ptM) or the other compounds (50 pM). The overlying medium was then removed and placed into y-counting tubes. The cells were removed from the petri dishes in 1 ml of BSS using a plastic spatula and transferred to a separate set of y counting tubes. 30 The Fe chelation efficacy of the QCIH and PKIH analogues was examined by comparing their activity to DFO and the corresponding PCIH analogue. The ability of the compounds to mobilise 59Fe from SK-N-MC neuroepithelioma cells was assessed after a 3 h labelling period with 59 Fe-Tf (0.75 FtM) at 37*C. The cells were then washed and WO 2004/069801 PCT/AU2004/000132 45 reincubated for 3 h at 37'C in the presence and absence of the internal standard DFO (100 ptM) or the other chelators at 50 ptM (Fig. 3 and Fig. 4). DFO was used at a concentration of 100 .tM due to the low efficacy of this chelator at mobilising "Fe from cells and preventing 5 9 Fe uptake from 59 Fe-Tf. 5 In general, the PKIH series showed similar or greater ability to mobilise 59 Fe from prelabelled cells, than the corresponding PCIH control compound (Fig. 3A). The greatest difference in activity between the PKIH and PCIH groups was found when comparing the more hydrophilic compounds from these series (see Table 1), i.e., PCAH and PKAH or PCHH and PKHH. 10 In general, the comparative QCIH analogues did not behave in a similar manner to the PCIH or PKIH analogues and showed little activity in terms of inducing 59 Fe mobilization (Fig. 3B). The most effective chelator of the QCIH series was the parent compound QCIH, which exhibited comparable cellular 59Fe mobilisation to PCIH (Fig. 3B). 15 Analysis of the "Fe mobilisation data for these three groups of compounds suggested that the dipyridyl substituent of the PKIH series appears to confer on the chelators high Fe chelation activity irrespective of the hydrazide substitution (Fig. 3A). In contrast, the presence of a single quinoline ring in the comparative QCIH series of compounds appears to result in low Fe chelation activity (Fig. 3B). 20 Example 3b - Comparison of the Effect of the PCIH, QCIH and PKIH Chelators on Cellular Iron Uptake from 59 Fe-labelled Transferrin Methodology: Iron Uptake Assay The ability of PKIH analogues to inhibit cellular uptake of 59 Fe uptake from 59 Fe-Tf 25 was studied using standard procedures.1 8 SK-N-MC Cells were incubated for 3 h at 37 0 C in media containing 59 Fe-Tf (0.75 tM) and either DFO (100 pM) or a compound of the of the PCIH, QCIH or PKIH series (50 pM). The cells were then placed on ice and washed four times with ice-cold Hank's balanced salt solution (BSS) to remove non-specifically bound 59 Fe-Tf. Cells were then incubated with the general protease, pronase (1 mg/ml), 30 for 30 min at 4 0 C to remove membrane-bound 59 Fe and Tf. All PKIH analogues were very effective at inhibiting 59 Fe uptake from 59 Fe-Tf (Fig. 4A). In particular, the PKAH and PKHH ligands showed marked activity while the corresponding control PCIH analogues, PCAH and PCHH, showed little effect (Fig. 4A). In general, the QCIH ligands were less effective than the PCIH and PKIH analogues at 35 preventing 59 Fe uptake from 59 Fe-Tf (Fig. 4B).

WO 2004/069801 PCT/AU2004/000132 46 Example 3c - Effect of PKIH Analogue Concentration on Cellular Iron Mobilization and Iron Uptake from Transferrin Methodology: Iron Mobilisation Assay 5 The efficacy of PKIH analogues on 5 9 Fe mobilisation was assessed as a function of compound concentration (Fig. 5A). Cells were prelabelled with 59 Fe-Tf (0.75 pLM) for 3 h at 37*C, washed, and then reincubated for 3 h at 37'C with a PKIH compound at a range of concentrations (0.5-50 [LM). Similarly, the effect of chelator concentration on 5 9 Fe uptake from 59 Fe-Tf was io assessed by incubating the PKIH analogues (0.5-50 pM) with 59 Fe-Tf (0.75 [LM) for 3 h at 37*C and the cells then washed (Fig 5B). PCTH was used as an internal control. PKIH, PKTH, and PKBH, were more effective than PCTH at mobilising cellular 59Fe at concentrations less than 10 pM (Fig. 5A). For instance, at a concentration of 1 pM, PKTH released 26% of cellular 59 Fe, whereas PCTH only released 10% (Fig. 5A). is Similarly, PKIH, PKBH and PKTH, had higher efficacy than the control PCTH at preventing 59 Fe uptake from 59 Fe-Tf (Fig. 5B). The more hydrophilic ligands, PKAH and PKHH (Table 1), exhibited lower efficacy in the "Fe uptake and 59 Fe mobilisation assays. Example 3d - The Effect of DpT Analogues on Iron Mobilisation from Prelabelled 20 SK-N-MC Neuroepithelioma Cells Methodology: Iron Mobilisation Assay Apotransferrin (Sigma) was labelled with 59 Fe (Dupont-NEN, MA, USA) to produce 59 Fetransferrin ( 59 Fe-Tf) using standard procedures.

1 8 Cells were prelabelled for 3 h at 37 0 C with 59 Fe-Tf (0.75 RM), washed, and then reincubated for 3 h at 37 0 C with the 25 chelators (25 pM; Fig. 11A). DFO and 311 were used as internal standards. The ability of the DpT analogues to directly mobilise 59 Fe from SK-N-MC cells was examined (Fig. 11A). DpT and Dp2mT resulted in the efflux of 6-7% of total cellular 59 Fe, while control cells reincubated with media alone released 5% of 59 Fe (Fig. 1 1A). The compound Dp2mT acts as a negative control as the presence of the methyl group at 30 the 2-position of the thiosemicarbazone hinders iron chelation. DFO only resulted in the mobilisation of 11% of cellular 5 9 Fe (Fig. 11 A). The other DpT compounds studied were significantly (p < 0.0001) more effective than DFO at mobilising 5Fe, and resulted in the release of 37-47% of cellular 59 Fe (Fig. 11). Dp44mT was the most efficient, resulting in the release of 47% of 59Fe (Fig. 11). The efficacy of the DpT analogues at inducing 59Fe 35 efflux correlated well to their anti-tumour activity (Example 4d, Table 3).

WO 2004/069801 PCT/AU2004/000132 47 Example 3e - The Effect of DpT Analogues on Cellular Iron Uptake from 59 Fe-Labelled Transferrin by SK-N-MC Neuroepithelioma Cells Methodology: Iron Uptake Assay 5 The ability of DpT analogues to inhibit cellular uptake of 59 Fe from 5 9 Fe-Tf was studied using standard procedures.

18 The amount of 59 Fe internalised by the cells was measured by incubation with the general protease pronase (1 mg/ml) for 30 min at 4'C to remove membrane-bound 59 Fe and Tf. The ability of DpT analogues to inhibit 59 Fe uptake from 59 Fe-Tf (0.75 pM) by SK 10 N-MC cells was examined in the absence or presence of a range of DpT analogues (25 pM) for 3 h at 37'C (Fig. 11B). In agreement with their low ability to mobilise 59 Fe, DpT and D2mT, had little effect at preventing 59 Fe uptake. The most efficient compounds examined, including 311 and Dp44mT, limited 59Fe uptake to 5-9% of the control, their activity being significantly greater (p < 0.0001) than DFO (Fig. 1 1B). These results agree 15 with the ability of these ligands to mobilise cellular 59 Fe (Fig. 11 A). All DpT analogues tested (except DpT and Dp2mT) showed much greater efficacy than DFO in preventing 59Fe uptake from 59 Fe-Tf by cells (Fig. 1 IB). The ability of DpT analogues to inhibit tumour cell growth (Table 3) was shown to correlated with their efficacy to induce 59 Fe release (Fig. 11 A) and prevent 59Fe uptake 20 (Fig. 11 B). The Fe chelation activity of Dp44mT, Dp4mT, Dp4eT, Dp4aT and Dp4pT was much greater than that mediated by DFO. Example 3f - The Effects of Dp44mT on Iron Uptake from Tf and Iron Mobilisation from M109 Cells 25 The ability of the compound Dp44mT to inhibit the growth of M109 lung carcinoma cells in mice was assessed. Studies were performed using M109 carcinoma cells in culture to determine the effect of Dp44mT on mobilising 59 Fe from these cells (Fig. 11 C) and inhibiting 5 9 Fe uptake from 59 Fe-Tf (Fig 11 D). As internal controls, cells were incubated with DFO and 311. The effect of compound concentration on increasing 30 59 Fe mobilisation from M109 cells was examined by labelling cells with 59 Fe-Tf (0.75 gM) for 3 h at 37 0 C followed by washing and reincubation with the respective compounds (0.2-25 ptM) for 3 h at 37*C (Fig. 11C). DFO was the least effective compound examined, and at 25 pLM released only 12 ± 1% of 59 Fe from cells, whereas control medium released 8 ± 1% (Fig. 11C). In contrast, 311 increased cellular 5 9 Fe WO 2004/069801 PCT/AU2004/000132 48 release from 8 + 1% (control medium) to 60 ± 1% at 5 pM (Fig 11 C). For Dp44mT 59 Fe release plateaued at 2 RM, resulting in the efflux of 48 ± 2 % of 59 Fe (Fig 11 C). The compound 311 was the most active compound studied at inhibiting cellular 59 Fe uptake from 59 Fe-Tf (0.75 pM) by M109 cells (Fig. 11D). At 1 ptM, 311 reduced 59 Fe 5 uptake to 40 + 4% of the control. In contrast, DFO had little effect. Dp44mT had similar activity to 311 up to a concentration of 5 gM, where it reduced 59 Fe uptake to 30 ± 1% of the control (Fig. 1 1D). EXAMPLE 4 - Inhibition of Cellular Proliferation In Vitro 10 Example 4a - Effect of PKIH Analogues on the Proliferation of SK-N-MC Neuroepithelioma Cells Methodology: Cellular Proliferation Assay The effect of compounds of the PCIH, QCIH and PKIH series on the proliferation of the SK-N-MC neuroepithelioma cell line (Table 2) was studied. The effect of the is compounds on the proliferation of SK-N-MC neuroepithelioma cells was examined using the MTT assay (described above). MTT colour formation was directly proportional to the number of viable cells measured by Trypan blue staining. In all experiments, the compounds 311 and DFO were used as relevant internal controls. 20 Table 2: The effect of compounds of the PCIH, QCIH and PKIH series of Fe chelators on the proliferation of SK-N-MC neuroepithelioma cells using the MTT assay.

IC

50 Values of Chelators (pM) PCIH Analogues QCIH Analogues PKIH Analogues DFO >50 DFO >50 DFO >50 311 3±2 311 3±2 311 3±2 PCIH >50 QCIH 49±3 PKIH 2±1 PCTH 48±4 QCTH >50 PKTH 3±1 PCBH 46±7 QCBH 50±1 PKBH 3±1 PCBBH 40±12 QCBBH 49±3 PKBBH 1±1 PCAH >50 QCAH >50 PKAH 42±9 PCHH >50 QCHH >50 PKHH 38±10 Data is expressed as Mean ± SD from 4 experiments. Cytotoxicity of the PKIH analogues was apparent from the morphological 25 appearance of the cells and the marked decrease in cell numbers. The PKIH analogues WO 2004/069801 PCT/AU2004/000132 49 showed very high anti-proliferative activity even at very low chelator concentrations (Table 2). The compounds, PKIH, PKTH, PKBH and PKBBH demonstrated very similar anti-proliferative activity to 311 (Table 2). In contrast to the PKIH analogues, the comparative PCIH and QCIH analogues had 5 little effect on cellular proliferation (Table 2). All compounds of the PCIH and QCIH series had IC 50 values of 40 pM to greater than 50 M. These results were in strong agreement with examination of cell morphology by phase contrast microscopy and Trypan blue staining which demonstrated the PCIH and QCIH series of compounds showed little cytotoxic activity. 10 Example 4b - Effect of Fe(III) Complexes of PKIH Analogues on the Proliferation of SK-N-MC Neuroepithelioma Cells Experiments were performed to determine whether the PKIH analogues were effective at inhibiting SK-N-MC cellular proliferation when complexed with Fe(III). At a 15 chelator to Fe molar ratio of 1:1 and 2:1, all of the PKIH-Fe complexes showed a similar level of anti-proliferative activity as that found for the free ligands (data not shown). As a relevant control in the same studies, the 1:1 and 2:1 compound to Fe complexes of 311 were also prepared and assessed. The Fe complexes of 311 had no significant effect on proliferation at concentrations up to 50 pM (data not shown). 20 Example 4c - Comparison of the Effect of PKIH Analogues on Proliferation of Fibroblasts and SK-N-MC Neuroepithelioma Cells The effect of PKIH, PKTH, PKBH and PKBBH on the growth of SK-N-MC neuroepithelioma cells and MRC-5 fibroblasts was studied (Fig. 6). The MRC-5 cell type 25 is a normal human lung fibroblast cell strain that is mortal and senesces after a number of passages in vitro. The SK-N-MC neuroepithelioma cell type is immortal. The effect of the PKIH analogues at inhibiting proliferation was increased in SK-N-MC cells than fibroblasts. For instance, while the PKIH analogues had IC 50 values of 1-3 pM in SK-N MC cells (Table 2), the IC 50 values in fibroblasts for PKBBH, PKBH, PKTH and PKIH 30 were 8, 11, 16 and >25 pM respectively. Example 4d - Effect of DpT Analogues on Proliferation of SK-N-MC Neuroepithelioma Cells The ability of DpT analogues (Fig. 2A) to inhibit proliferation of SK-N-MC 35 neuroepithelioma cells was assessed (Fig 10). Compounds (0-25 gM) were incubated WO 2004/069801 PCT/AU2004/000132 50 with cells for 72 h and at the end of this incubation period, cell density was determined by the MTT assay, as described above. Dp2mT served as a negative control. Data obtained using the MTT assay directly correlated with cell numbers estimated via light microscopy. 5 Dp44mT, Dp4eT, Dp4aT and Dp4pT showed very high anti-proliferative activity

(IC

50 = 0.03-0.06 pM; Table 3). These DpT analogues were significantly (p < 0.0001) more active than DFO (IC 50 = 5 tM) and have greater efficacy than other Fe chelators such as 311 (IC 50 = 0.3 RM; Table 3). Compared to the anti-proliferative activity of the established cytotoxic agent, doxorubicin, in SK-N-MC cells (IC 50 = 0.02 piM; Table 3), 10 Dp44mT, Dp4eT, Dp4aT and Dp4pT have similar efficacy. The iron chelator, Triapine* (Fig. 1B) had less activity (IC 50 = 0.26 pLM; Table 3) than the above DpT analogues. Dp4mT also demonstrated appreciable activity against SK-N-MC cells and was comparable to Triapine*, having an ICso value of 0.19 pM (Table 3). 15 Table 3: The effect of Fe-chelators on cellular proliferation of neoplastic and normal cells

IC

50 (pM) Neoplastic Cells Normal Cells SK-N-MC SK-Mel-28 MCF-7 MRC-5 neuroepithelioma melanoma breast cancer fibroblasts DFO 5 2 15 7 14 9 >25 311 0.30 0.2 0.9 0.5 * >25 311m 0.80±0.2 1.4 0.4 0.9 0.4 >25 Dp3ll 0.6 ±0.04 1.1 0.5 1.6 0.1 >25 DpT >25 >25 >25 >25 Dp2mT >25 >25 >25 >25 Dp4mT 0.19 0.1 0.6 ±0.5 0.3 ±0.2 >25 Dp44mT 0.03 0.01 0.06 ± 0.03 0.06 ± 0.01 >25 Dp4eT 0.06 0.01 0.09 ± 0.06 0.08 ± 0.01 >25 Dp4aT 0.06 0.01 0.10 ± 0.06 0.07 ± 0.01 >25 Dp4pT 0.05 ± 0.006 0.09 ± 0.05 0.07 0.01 >25 Triapine 0.26 ± 0.01 2.6 ± 0.6 3.0 ± 1.5 * Dox 0.02 0.01 0.35 ± 0.09 0.6 ± 0.2 * Results are mean i SD (at least 5 experiments). * Data not available DpT analogues were also assessed against the SK-Mel-28 melanoma and MCF-7 20 breast cancer cell lines (Table 3). Dp2mT served as a negative control as the methyl WO 2004/069801 PCT/AU2004/000132 51 group at the 2-position hinders Fe-chelation. The compounds Dp4mT, Dp44mT, Dp4eT, Dp4aT and Dp4pT were each more active (p < 0.0001) than DFO at inhibiting proliferation of SK-Mel-28 melanoma and MCF-7 breast cancer cells (Table 3). Against these cell lines, Dp44mT, Dp4eT, Dp4aT and Dp4pT were the most active (IC 50 = 0.06 5 0.10 gM; Table 3), Dp44mT being the most effective anti-proliferative agent when assessing all neoplastic cell types. Overall, the efficacy of the active DpT analogues was greater than that of 311 and Triapine*, and better than that found for doxorubicin (Table 3). Significantly, in contrast to the marked anti-proliferative activity of Dp44mT, Dp4eT, Dp4aT and Dp4pT against immortal neoplastic cells, those compounds showed 10 surprisingly little effect on the proliferation of mortal MRC-5 fibroblasts (IC 50 values >25 riM) (Fig. 10 and Table 3). Example 4e - Comparison of Inhibition of Proliferation for a Range of Cell Types The compounds 311, DFO, DOX, PKIH, PKTH, Dp44mT, Dp4eT and Dp4pT (0 50 pM) were incubated with cells for 72 h. At the end of the incubation period, cell 15 density was determined by the MTT assay (described above). Results are shown in Table 4. Table 4 - The effect of Fe-chelators on cellular proliferation of a range of neoplastic cells, including, H69AR lung cancer, IMR-32 Neuroblastoma, HEP G2 Hepatoma, DU 145 prostate cancer, and NCI H2452 mesothelioma cell lines. 20

_IC

5 0 (pM) H69AR A IMR-32 A HEP G2A DU 145* NCI H2452" Lung Neuroblastoma Hepatoma Prostate Mesothelioma Cancer Cancer 311 0.97 0.30 1.18 1.94 0.47 DFO >50 9.38 18.7 15.75 32.3 DOX 0.39 0.01 2.61 0.09 0.17 PKIH -- 0.38 1.41 0.45 0.86 PKTH 0.78 0.30 1.17 1.43 0.73 Dp44mT 0.05 0.01 1.36 0.06 0.01 Dp4eT -- 0.01 0.02 -- 1.17 Dp4pT 0.97 0.01 0.08 0.53 0.01 A 1 experiment; * mean SD of 2 experiments; # mean SD of 3 experiments The anti-proliferative activity of the more effective compounds on a range of tumour cell lines derived from a number of very aggressive cancers was assessed. In these experiments, DFO and 311 were used as appropriate standards. In addition, these studies WO 2004/069801 PCT/AU2004/000132 52 included the cytotoxic agent, doxorubicin (DOX), which is one of the most effective anti tumour agents in current clinical use. The least effective chelator examined was the internal standard, DFO, which is the current drug in clinical use for the treatment of iron overload disease. The most effective compounds examined included the 3 DpT analogues, 5 namely, Dp44mT, Dp4eT and Dp4pT. Notably, the activity of these DpT analogues in some cancer cell lines was more pronounced than that of DOX. For instance, in the hepatoma cell line, HepG2, the IC 50 of Dp4eT was 0.02 pLM which is over 10-fold less than that found for DOX (2.61 jiM). The activity of the PKIH analogues, PKIH and PKTH, was also impressive, being similar to 311 and slightly less effective than DOX. 10 Importantly, the DpT and PKIH analogues work by a very different mechanism of action to DOX, and may possibly be useful in cancer cell types resistant to DOX. EXAMPLE 5 - In Vivo Dose-Dependent Inhibition of the Growth of the M109 Lung Carcinoma 15 General Methodology Determination of the Maximum Tolerated Dose in CD2F1 Hybrid Mice. BALB/c and DBA/2 mice were obtained from Animal Resources Centre (University of New South Wales (UNSW), Sydney, Australia) and crossed to produce CD2F1 animals (all procedures used were approved by the Animal Ethics Committee, 20 UNSW). Groups of eight female CD2F1 mice (8-10 weeks old) were fed and watered ad libitum, and injected via the tail vein with compounds being studied in 30% propylene glycol in 0.9% sterile saline, twice a day, 6 h apart, 5 days/week for 2 weeks. The maximum tolerated dose (MTD) was defined as the dose at which 30% of a cohort either died or lost weight in excess of 10%.19 25 Inhibition of M1 09 Lung Cancer Cell Growth by Iron Chelators In Vivo Female CD2F1 mice aged 8-10 weeks old were se implanted with 1 x 10' M109 cells. The tumour cells were passaged in CD2F1 mice by preparing a brei from the tumour and injecting it sc.

20 Dp44mT was dissolved in 30 % (v/v) propylene glycol in saline and injected iv 4 days after tumour engraftment, twice daily for 5 days followed by 30 a "rest period" of 2 days when the animal was not injected. On the twelfth day after tumour implantation, mice were sacrificed using methoxyfluorane and cardiac puncture performed. Blood counts and erythrocyte indices were measured using a Sysmex Blood Counter (Prince of Wales Hospital, Sydney, Australia). Mouse body weight changes during the treatment period were recorded. The tumours were weighed and fixed for WO 2004/069801 PCT/AU2004/000132 53 histological examination. The experimental groups consisted of eight mice for the control and each treatment (Table 5). Figure 12A shows the effects of 0.15, 0.3, and 0.4 mg/kg Dp44mT (MTD = 0.4 mg/kg) on tumour growth after iv injection twice daily for 5 consecutive days from day 4. 5 As a relevant positive control, animals were administered Triapine* at its MTD (6 mg/kg). The tumour weight was measured on the twelfth day after the tumour cell implantation. Table 5. Mouse weight loss and haematological indices from animals treated with 10 vehicle alone (control), Dp44mT (0.15, 0.3 and 0.4 mg/kg), or Triapine@ (6 mg/kg). Control Dp44mT Triapine@ (mg/kg) (6 mg/kg) 0.15 0.3 0.4 Body weight loss 0.83 ± 0.3 1.4 ± 0.2 1.3 ± 0.3 1.1 ± 0.2 5.3 ± 0.4 (g) WBC (10 9 /L) 4.4 ±0.4 4.2 ± 0.8 4.2 ± 0.4 3.9 ± 0.6 1.4 ± 0.3 RBC (10 12 /L) 9.1 ± 0.2 9.4 ± 0.1 8.9 ± 0.4 8.0 ± 0.6 6.1 ± 0.4 Haemoglobin 13.7 ± 0.2 14.0 ± 0.2 13.3 ± 0.5 11.8 ± 0.8 8.6 ± 0.5 (g/dL) Haematocrit 0.46 ± 0.01 0.46 ± 0.01 0.40 ± 0.03 0.40 ± 0.01 0.28 ± 0.01 Platelets (10 9 /L) 788±47 1047 ± 42 1019 ±87 636 ± 88 976 171 Results are Mean + SEM (3 separate experiments). The experimental groups consisted of eight mice for the control and each treatment. Administration of Dp44mT inhibited tumour growth in a dose-dependent manner 15 (Fig. 12A) and at 0.4 mg/kg reduced tumour weight to 47 % (p < 0.01) of control values. There was no significant difference in mouse body weight loss or leukocyte cell count in the control group compared to animals treated with Dp44mT (Table 5). However, compared to the control, a slight (p < 0.05) decrease in haemoglobin, hematocrit, erythrocyte and platelet counts were observed in animals treated with Dp44mT at its 20 MTD (0.4 mg/kg) but not at lower doses (Table 5). Administration of Dp44mT at 0.15 and 0.3 mg/kg increased platelet counts (Table 5). Triapine* was more effective than Dp44mT, significantly (p < 0.001) reducing tumour weight to 10% of the control (Fig. 12A). However, in contrast to Dp44mT, Triapine* at its MTD (6 mg/kg) markedly and significantly (p < 0.0001) decreased animal weight, haemoglobin concentration, WO 2004/069801 PCT/AU2004/000132 54 hematocrit, erythrocyte and leukocyte cell counts (Table 5), suggesting that under these conditions Triapine@ was systemically toxic. EXAMPLE 6 - Effect of PKIH Analogues on 3 H-Thymidine, 3 H-Leucine, and 5 3 H-Uridine Incorporation Methodology: 3 H-Thymidine, 3 H-Leucine, and 3 H-Uridine Incorporation Assay After a 20 h incubation of the SK-N-MC cells with a selection of analogues, 3

H

thymidine, 3 H-leucine, or 3 H-uridine (1 pCi/ml) was added for 2 h at 37 0 C. The cells were detached from the 96 well plate using 1 mM EDTA in Ca(II)/Mg(II)-free PBS and 10 collected and washed using a cell harvester (Tomtec Harvester 96, Linbrook, Queensland, Australia). Radioactivity was measured on a p-scintillation counter (LKB Wallace, Turku, Finland). Example 6a - Effect of Compounds of the PCIH, QCIH and PKIH Series on 3 H-Thymidine Incorporation 15 The effect of compounds of the PKIH series on 3 H-thymidine incorporation was compared to the corresponding PCIH and QCIH analogues and also to DFO and 311 as relevant controls (Table 6). Table 6: The effect of compounds of the PCIH, QCIH and PKIH series on 3 H-thymidine 20 incorporation by SK-N-MC neuroepithelioma cells. 3 H-Thymidine Incorporation (% Control) PCIH Analogues QCIH Analogues PIH Analogues Control 100 ±7 Control 100 ±7 Control 100± 7 DFO 29± 13 DFO 29± 13 DFO 29± 13 311 0.1 ±0.1 311 0.1 ±0.1 311 0.1 ±0.1 PCIH 43 ± 1 QCIH 77 ± 9.5 PKIH 0.06 ± 0.02 PCTH 64± 14 QCTH 83 ±6 PKTH 0.04 ± 0.01 PCBH 72 ± 16 QCBH 64 ±5 PKBH 0.03 ± 0.01 PCBBH 33 ± 6 QCBBH 81 ± 10 PKBBH 0.02 ± 0.01 PCAH 12 ±1 QCAH 43 ±2 PKAH 23 ±10 PCHH 11 ±2 QCHH 41±2 PKHH 25± 4 Data is expressed as Mean ± SD from 4 experiments.

WO 2004/069801 PCT/AU2004/000132 55 PKIH, PKTH, PKBH, and PKBBH, were the most effective compounds examined in terms of their ability to inhibit 3 H-thymidine incorporation, showing similar or slightly greater activity than the ribonucleotide reductase inhibitor, 311 (Table 6). In fact, there 5 was almost undetectable levels of 3 H-thymidine in cells treated with the PKIH analogues. For instance, PKBBH reduced 3 H-thymidine incorporation to 0.02 % of the control (Table 6). Several of the more hydrophilic PKIH analogues, namely PKAH and PKHH (Table 1), showed significantly (p < 0.0001) less activity, inhibiting 3 H-thymidine incorporation to 23 % and 25 % of the control respectively (Table 6). However, the relatively low 10 efficacy of these latter compounds at inhibiting 3 H-thymidine incorporation cannot solely be attributed to their hydrophilicity, as the most hydrophilic analogue of this series (PKIH; Table 1) had very high activity (Table 6). In comparison to 311 and the PKIH analogues, the QCIH analogues showed considerably less activity at inhibiting 3 H-thymidine incorporation, reducing is incorporation to 41-83% of the control (Table 6). Example 6b - Comparison of Compounds of the PCIH and PKIH Series on 3 H-Leucine and 3 H-Uridine Incorporation Considering the marked effects of the PKIH group of analogues on 3 H-thymidine 20 incorporation, the effect of these compounds on 3 H-leucine (Table 7) and 3 H-uridine (Table 8) incorporation was also assessed. The results obtained were compared to the PCIH series of analogues which show high Fe chelation activity but low anti-proliferative effects. Table 7: The effect of the PCIH, and PKIH ligands on 3 H-leucine incorporation by 25 SK-N-MC neuroepithelioma cells. 3 1H-Leucine Incorporation (% Control) PCIH Analogues PLIH Analogues Control 100 ±7 Control 100± 7 DFO 9±1 DFO 9 ±1 311 2 ±1 311 2 ±1 PCIH 74 ±7 PKIH 26 ±1 PCTH 21 ±6 PKTH 19± 8 PCBH 30 ±13 PKBH 21± 2 WO 2004/069801 PCT/AU2004/000132 56 PCBBH 16 ± 5 PKBBH 8 ±1 PCAH 76 ±2 PKAH 25 2 PCHH 80± 10 PKHH 22 0.5 Data is expressed as Mean ± SD from 4 experiments. Table 8: The effect of the PCIH, and PKIH ligands on 3 H-uridine incorporation by SK-N-MC neuroepithelioma cells. 5 3 H-Uridine Incorporation (% Control) PCIH Analogues PKIH Analogues Control 100 ±2 Control 100 ± 2 DFO 34 ±5 DFO 34 ± 5 311 5 ±2 311 5± 2 PCIH 50 ±8 PKIH 19 ±1 PCTH 43 ±2 PKTH 5 ±1 PCBH 31 ±9 PKBH 5± 1 PCBBH 47 ±2 PKBBH 3 ±1 PCAH 40 ± 9 PKAH 28 ±1.5 PCHH 41 ±11 PKHH 21 ±1.5 Data is expressed as Mean SD from 3 experiments. The most active of the PKIH analogues in terms of inhibiting 3 H-thymidine incorporation, namely PKIH, PKTH, PKBH and PKBBH, were significantly (p < 0.0001) less effective at preventing the incorporation of 3 H-leucine and 3 H-uridine into protein 10 and RNA respectively (Tables 7 and 8). Further, these PKIH analogues had significantly (p < 0.001) less effect than 311 on 3 H-leucine incorporation (Table 7). In general, the PIH analogues were more effective than the PCIH analogues at preventing 3 H-leucine or 3 H-uridine incorporation (Tables 7 and 8). PKBBH showed the greatest activity at inhibiting 3 H-leucine or 3H-uridine incorporation. 15 EXAMPLE 7 - Effect of the PKIH Analogues on the Expression of WAF1 and GADD45 mRNA Methodology: Northern Blot Analysis Northern blot analysis was performed by isolating total RNA using the Total RNA 20 Isolation Reagent (Advanced Biotechnologies Ltd, Surrey, United Kingdom). The WO 2004/069801 PCT/AU2004/000132 57 membranes were hybridised with probes specific for human WAF1, GADD45, and p actin. The WAFi probe consisted of a 1 kb fragment from pSXV (ATCC; Cat. No. 79928). The GADD45 probe consisted of a 760 bp fragment from human GADD45 cDNA cloned into pHul45B2 (kindly supplied by Dr. Albert Fornace, National Cancer 5 Institute, NIH, Maryland). The P-actin probe consisted of a 1.4 kb fragment from human p-actin cDNA cloned into pBluescript SK- (ATCC; Cat. No. 37997). The effect of PKIH analogues on the expression of the genes WAF1 and GADD45 which encode cell cycle inhibitors essential for Gi-S arrest was assessed. The internal controls DFO, PCBBH, and 311, caused up-regulation of GADD45 and WAF1 mRNA 10 expression, while PCBH and PCIH showed little effect (Fig. 7). In good correlation with their marked anti-proliferative activity, PKIH, PKTH, PKBH and PKBBH had a pronounced effect, significantly (p < 0.0001) increasing GADD45 and WAF1 mRNA levels when compared to the control. The PKIH analogues were significantly (p < 0.01) more potent at inducing WAF1 and GADD45 mRNA expression than 311 (Fig. 7). 15 EXAMPLE 8 - Induction of Ndrgl mRNA Expression MCF-7 cells were incubated for 24 h with a range of metal ion chelators. The cell membrane impermeable chelator, EDTA (250 ptM) does not show anti-proliferative effects (IC 5 0 > 250 ptM), and was compared to more permeable metal chelating compounds which show low and high anti-proliferative activity, respectively. The 20 compounds with low anti-proliferative activity in MCF-7 cells, include DFO (IC 50 = 20 pfM), Li (deferiprone; IC 5 o = 180 iM) and dipyridyl (IC 50 >250 ptM) and these were assessed at 25 and 250 ptM. The compounds 311 and dp44mT demonstrate high anti tumour efficacy against MCF-7 cells (IC 50 = 0.6 ptM and 0.1 jiM, respectively), and were assessed at 25 pM (Fig. 16) Penicillamine is a well known copper chelator and was 25 assessed for comparison. Dp2mT was used as a negative control. At a concentration of 25 ptM, DFO, penicillamine (PEN), dp2mT, EDTA, dipyridyl and L1 had no effect at up-regulating Ndrg] (Fig. 16). In contrast 311 and dp44mT markedly increased Ndrg1 levels (Fig. 16). EXAMPLE 9 - The Effect of PKIH Analogues on Iron Regulatory Protein 30 RNA-Binding Activity Methodology: Gel-retardation Assay The gel-retardation assay was used to measure the interaction between the iron regulatory proteins (IRPs) and iron-responsive element (IRE) using established WO 2004/069801 PCT/AU2004/000132 58 techniques.21 Cells were incubated with DFO (100 pM), FAC (100 tg/ml) and PKIH analogues (25 pM) for 20 h at 37*C and cell lysates prepared. The IRP-RNA-binding activity was then assessed by the gel-retardation assay. Aliquots of cell lysate containing 2 tg of protein were incubated with 0.1 ng (approximately 1 x 10 5 cpm) of 32 P-labelled 5 riboprobe for 30 min at room temperature. Using this protocol the level of RNA added was saturating. Unprotected probe was degraded by incubation with 1 U of RNAse TI for 10 min at room temperature. Heparin (5 mg/ml) was then added for another 10 min to exclude non-specific binding. The IRE-protein complexes were analyzed on 6 % non denaturing polyacrylamide gels. In parallel experiments, samples were treated with 10 ferricyanide (FeCN, 5 mM) and 0.5% p-mercaptoethanol (P-ME), prior to addition of the IRE probe. This procedure results in the conversion of IRP1, whether spontaneously active or not, into an active IRE-binding form. The IRP-IRE mechanism is a major regulator of cellular Fe homeostasis that responds to Fe chelation. Accordingly, the effect of PKIH analogues on IRP-RNA 15 binding activity was determined (Fig. 8). SK-N-MC cells were incubated with the chelators (25 pM) for 20 h at 37 0 C. The Fe chelators DFO (100 pIM), 311 (25 pM), or the Fe donor ferric ammonium citrate (FAC; 100 [tg/ml), were used as relevant controls in all experiments.

3 23 PCAH was used as a further control as it was known to reduce IRP-RNA binding activity.

24 20 A significant (p < 0.005) increase in IRP-RNA binding occurred in cells incubated with DFO and 311, while FAC and PCAH significantly (p < 0.001) decreased RNA binding (Fig. 8). Compared to the control, there was a significant (p < 0.01) increase in IRP-RNA binding activity after treatment of cells with the PKIH series (Fig. 8), reflecting their ability to bind cellular Fe pools. Addition of p-mercaptoethanol and FeCN to the cell 25 lysates demonstrated no change occurred in the total amount of IRP-RNA binding activity after incubation with all the chelators (data not shown). EXAMPLE 10 - INDUCTION OF APOPTOSIS Example 10a - Increased apoptotic cells in Dp44mT-treated tumour samples from mice 30 and the examination of apoptosis in cultured M109 cells after incubation with Dp44mT Apoptosis Assay Using Flow Cytonetry Apoptotic cells were detected by a flow cytometer (FACSCalibur, Becton Dickinson, CA, USA) using fluorescein isothiocyanate (FITC) labelled Annexin V binding to translocated plasma membrane phosphatidylserine (PS).

25 Propidium iodide WO 2004/069801 PCT/AU2004/000132 59 (PI) was added to identify loss of cell membrane integrity, which is indicative of necrotic cells. DNA cleavage was assessed by the terminal deoxyribonucelotidyl transferase mediated dUTP nick end-labelling (TUNEL) reaction.

26 Using the in situ TUNEL assay, 5 tumour samples from animals treated with Dp44mT (0.4 mg/kg) (Fig. 12B(ii)) showed clear evidence of increased apoptotic cell death compared to vehicle-treated controls (Fig. 12B(i)). To further investigate the apoptotic pathways induced by Dp44mT, cultured M109 cells were incubated with Dp44mT for 24 h and analysed for apoptosis and death using Annexin V-FITC and PI staining, respectively. There was a dose-dependent 10 increase in the percentage of apoptotic and dead cells after Dp44mT treatment (Fig. 13A). Exposure of cells to 1 pLM Dp44mT caused a significant (p < 0.05) increase of apoptotic and dead cells. This increase peaked at a Dp44mT concentration of 100 piM resulting in a population consisting of 40 + 7% apoptotic cells and 27 ± 3% dead cells (Fig. 13A). After incubation with Dp44mT at 250 piM, apoptotic cell numbers decreased to 20 ± 2%, and 15 the dead cell population increased to 42 ± 6 %, suggesting dose-dependent cytotoxicity. Induction of apoptosis by Dp44mT (1 jiM) was also time-dependent (Fig 13B). After incubation with 1 gM Dp44mT for 12 h, a significant (p < 0.001) increase in apoptotic cells (12 ± 2 %) was detected. Thus, at 1 [tM Dp44mT markedly promoted 59Fe release (Fig. 1 1A,C), inhibited 59 Fe uptake (Fig. 1 1B,D), and induced apoptotic cell death 20 (Fig. 13B). Example 1Ob - Protein levels and activities of caspase-3, 8, and 9 in M109 cells after incubation with Dp44mT. General Methodology 25 Antibodies Rabbit anti-active caspase-3 antibody, rabbit anti-active caspase-8 antibody and Annexin V fluorescein isothiocyanate (FITC) were from BD PharMingen (San Diego, CA, USA). Anti-h-cytc MoAb was from R&D Systems (Minneapolis, MN, USA). Rabbit anti-active caspase-9 antibody, mouse anti-Bel-2 and mouse anti-Bax antibodies were 30 obtained from Santa Cruz (Santa Cruz, CA, USA). Mouse anti-mouse B-actin antibody, horseradish peroxidase-conjugated antirabbit, and anti-mouse IgG were from Sigma Chemical Co. (St. Louis, MO, USA).

WO 2004/069801 PCT/AU2004/000132 60 Caspase Activation and Activity Assay Cultured M109 cells were treated with or without Dp44mT (1 pM) for 0-48 h at 37'C. Cells were lysed 27 and caspase activities were determined using the BD Apolerti" Caspase Assay Plate (BD Biosciences, San Diego, CA, USA). Cell-permeable pan 5 caspase-3, -8, and -9 inhibitors (BD Biosciences) were included as inhibitory controls. To determine the role of caspase activation in Dp44mT-induced apoptosis in M109 cells, protein levels of active caspase-3, -8 and -9 (Fig. 14A,B) and their enzymatic activity (Fig. 14C,D) were examined. Short incubations of 2 h with Dp44mT (1 pM) increased the protein levels of caspase-3 and -9 to 168 and 171% of the control, 10 respectively, while caspase-8 was not increased. Incubation of M109 cells with Dp44mT (1 piM) for 6 h resulted in a pronounced increase of caspase-3 to 400% of the control, while the increase in caspase-9 and caspase 8 were less marked, being elevated to 280% and 150% of the control, respectively (Fig. 14A,B). An incubation of 12 h with Dp44mT resulted in caspase-8 protein levels is increasing to 400% of the control, while that of caspase-3 and -9 remained at approximately the same level as that found at 6 h (i.e., at 380% and 280% of the control, respectively). An incubation of 24 h with Dp44mT increased the protein level of all caspases to about 4-fold that of control cells, while after 48 h, caspase-3, -8 and -9 levels were 5.5-, 3.4- and greater than 10-fold that of control cells (Fig. 14A,B). 20 Caspase-3 activity increased most markedly after 6 h, and this occurred in a time dependent manner to 29-fold of that found for the control after 48 h (Fig. 14C). The activities of caspase-8 and -9 increased to a far lesser extent to 299% and 354% of the control after a 12 h incubation with Dp44mT (Fig. 14C). The activity of these latter enzymes plateaued at approximately 400-600% of the control after 24 h (Fig. 14C). 25 Membrane-permeable inhibitors of each caspase reversed their activation when incubated with Dp44mT (1 ptM), demonstrating the specificity of the observed activity (Fig. 14D. These results show that Dp44mT increased caspase-3 and -9 protein levels within 2 h, while the protein levels of caspase-8 were only slightly increased even after a 4 h or 6 h (Fig. 14A,B). The most striking increase in enzymatic activity was observed for caspase 30 3, which increased 300-fold compared to the control after 48 h incubation with Dp44mT (Fig. 14C). Example 10c - Release of holo-cytochrome c (h-cytc) from mitochondria into the ctosol of M109 cells after incubation with Dp44mT Due to the importance of h-cytc release from the mitochondrion on apoptosis 35 induction, 8 further studies investigated the effect of Dp44mT on this process (Fig. 15A).

WO 2004/069801 PCT/AU2004/000132 61 Preparation of cytosolic and mitochondrial fractions and western blotting were carried out using standard techniques.

27 There was very little h-cytc expression in the cytosolic fractions of control M109 cells (i.e., at 0 h), with the greatest amount of h-cytc being found in the mitochondrion at this time (Fig. 15A). However, after a 2 h exposure to 5 Dp44mT (1 RM), h-cytc was found in the cytosolic fraction of M109 cells, while there was a concomitant decrease in the mitochondrial level of h-cytc (Fig. 15A). The release of h-cytc into the cytosol plateaued after a 4 h incubation with Dp44mT and then decreased to that found in control cells after 24 and 48 h. This increase in cytosolic h-cytc correlated with the loss from the mitochondrion (Fig. 15A). The near complete 10 disappearance of h-cytc from the cytosol at 24 and 48 h may be due to its release from cells or its degradation 2 8 (Fig. 15A). Incubation with Dp44mT for only 2 h resulted in h-cyte release from the mitochondrion (Fig. 15A) and this occurred at a similar time or before activation of caspases-3, -8 and -9 (Fig. 14A-C). While note intending to be limited to a particular 15 mechanism of action, this observation is consistent with Dp44mT inducing the mitochondrial pathway of apoptosis and accounts, at least in part, for the marked activation of caspases. Example 10d - Expression of Bcl-2 and Bax in M109 cells after incubation with Dp44mT. 20 Considering the roles of Bcl-2 and Bax in the mitochondrial pathway of apoptosis and their role in h-cytc release, 8 ,"i, 12 the effect of Dp44mT (1 ptM) on the protein levels of these molecules was assessed (Fig. 15B). After incubation with Dp44mT (1 RM), the anti apoptotic molecule Bel-2 gradually decreased as a function of time, decreasing to 69% and 37% of the 0 h time point after a 2 and 48 h incubation, respectively (Fig. 15B). 25 Conversely, Bax expression only increased markedly after a 48 h incubation with Dp44mT, reaching 500% of that found at the 0 h time point (Fig. 15B). The release of h-cytc observed within 2 h (Fig. 15A) coincided with the decrease in the mitochondrial Bcl-2 expression (Fig. 15B), which is involved in preventing h-cytc release from mitochondria.

8 However, a marked increase in Bax expression which plays a 30 role in h-cytc release and caspase activation was only observed after 48 h (Fig. 15B).

8 The imbalance of Bcl-2 and Bax expression may have resulted in mitochondrial h-cytc release, as found under other conditions.

8 Considering h-cytc efflux from the mitochondrion, previous studies have demonstrated that oxidant stress is a potent inducer of its release as well as the down-regulation of Bel-2, the upregulation of pro-apoptotic 27 35 proteins (eg., Bax), and the activation of caspases.

WO 2004/069801 PCT/AU2004/000132 62 Example 10e - Increase of Intracellular ROS Generation after Incubation with Dp44mT Intracellular Reactive Oxygen Species (ROS) Assay Intracellular ROS generation was assessed using the cell-permeable dye 2',7' 5 dichlorofluorescein-diacetate (DCF-DA) (Sigma). DCF-DA readily diffuses into cells and becomes highly fluorescent when oxidised by 02', H 2 0 2 , or OH-.

29 Cellular fluorescence intensity is directly proportional to the level of intracellular ROS.

29 M109 cells (1 x 10 5 cells/well) were incubated with or without 1 pM Dp44mT for 0-48 h or 50 pM H 2 0 2 (positive control) for 10 min at 37"C. The cells were then incubated with DCF-DA (5 10 pM) for 20 min at 37'C, washed twice, and detached from the culture plates. The fluorescence of oxidised DCF in cells was measured using flow cytometry.

29 ) To test whether induction of h-cytc release from the mitochondrion (Fig 15A) and the imbalance of Bcl-2/Bax expression (Fig 15B) was due to the presence of ROS, intracellular ROS were measured by flow cytometry using the DCFH-DA probe which 15 becomes fluorescent when oxidised by 02', H 2 0 2 , or OH-.

29 Incubation of M109 cells with Dp44mT (1 pM) increased the levels of oxidised probe as a function of time. After 2 h, a 25 + 10% increase in oxidised DCFH-DA fluorescence was found compared to the 0 h time point, and this increased to 486 ± 96% after 48 h (Fig. 15C). For comparison, a 10 min incubation with the positive control, H 2 0 2 (50 tM), increased fluorescence to 1312 + 20 46% of the 0 h time point (Fig. 15C) In this study, an increase in ROS was detected after a 2 h incubation with Dp44mT (Fig. 15C) and this coincided with h-cytc release (Fig. 15A). These observations are consistent with a role of ROS in inducing h-cytc release, however other mechanisms cannot be excluded.

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WO 2004/069801 PCT/AU2004/000132 64 22. Abboud, S. & Haile, D.J. (2000). J. Biol. Chem. 275, 19906-19912. 23. Darnell, G. & Richardson, D.R. (1999). Blood, 94, 781-792. 24. Becker, E. & Richardson, D.R. (1999). J. Lab. Clin. Med., 134, 510-521 25. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. J Immunol 5 Methods. 1995;184:39-51. 26. Gavrieli Y, Sherman Y, Ben-Sasson SA. J Cell Biol. 1992;119:493-501. 27. Yuan J, Murrell GA, Trickett A, Wang MX. Biochim Biophys Acta. 2003;1641:35-41. 28. Jemmerson R, LaPlante B, Treeful A. Cell Death Differ. 2002;9:538-548 10 29. Wang M, Wei AQ, Yuan J, Trickett A, Knoops B, Murrell GAC. FEBS Lett. 2002;532:359-362. 30. El-Deiry, W.S., Tokino, Tl, Velculescu, V.E., Levy, D.B., Parsons, R., trent, J.M., Lin, D., Mercer, W.E., Kinzler, K.W., & vogelstein, B. (1993). Cell, 75, 817-825.

Claims (19)

1. A compound of Formula 3 (R4)nN -N E (R4) - HN N G or a pharmaceutically acceptable salt thereof, 5 wherein E isO or S; nis0, 1,2,3or4; pis0, 1,2,3or4; each R 4 is independently selected from halogen, alkyl, alkenyl, optionally substituted 10 amino, hydroxyl, -0-alkyl, -S-alkyl, -C(O)-alkyl, -C(O)-alkenyl, -C(O)-0-alkyl, nitro and cyano; -G is NR

2 R 3 or CR 2 R

3 R 5 , wherein the bond between C and R 5 may be a single bond, double bond or triple bond; and wherein when the bond between C and R 5 is a double bond, one of R 2 and R 3 is absent; and wherein when the bond between C and R 5 is a triple bond, R 2 and R 3 are both absent; 15 R 2 and R 3 may be the same or different and are individually selected from hydrogen, halogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted amino, optionally substituted heteroaromatic, an optionally substituted bicyclic group, and an optionally substituted aromatic group; and R 5 is selected from the group consisting of hydrogen, halogen, optionally substituted alkyl, 20 optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted amino, optionally substituted heteroaromatic, an optionally substituted bicyclic group, and an optionally substituted aromatic group; or G is an optionally substituted heteroaromatic group, an optionally substituted bicyclic group, an optionally substituted aromatic group, an optionally substituted alkyl group, an 25 optionally substituted cycloalkyl group, or an optionally substituted heterocycloalkyl group; with the provisos that: (i) when E is 0, n is 0 and p is 0, then -G is not -NH 2 , CH 2 CI, 2-furanyl, 2-thiophenyl; phenyl, 2-halophenyl, 3-halophenyl or

4-halophenyl, where halo is F, Cl, Br or I; 2-aminophenyl; 4-aminophenyl, 2-hydroxyphenyl; 30 4-hydroxyphenyl; 2-pyridyl; 4-pyridyl; 66 - CH 3 -N-N \ N-NHN--NH N /- NH2 N-HNH2 - - 00 (ii) when E is S, n is 0 and p is 0, then -G is not NH 2 , NHCH 3 , -NHPhenyl, -NH(benzyl); NH(benzyl) 2 ; -NH(2-pyridyl); -NH-CH 2 -(2 pyridyl); N(methyl) 2 ; N(ethyl) 2 ; N(propyl) 2 ; N(butyl) 2 ; N(pentyl) 2 ; N(hexyl) 2 ; N(methyl)phenyl; 5 -N-pyrrolidinyl; -N-piperidinyl; -N-morpholinyl; 2-thiophenyl; 2-furanyl; 2-hydroxyphenyl; N OH H HH0 H-N-N -N H H - HN N N -N-N N-N -\ S -N N4 CH 3 HN-N N O -N O-- N -N N-CH 3 N CH 3 , \ / or OH 3 N / SH N N'N CH 3 S N / ; and (iii) when E is S, n is 0 and p is 1, then: 10 (a) when R 4 is 6-Br, G is not N(methyl) 2 ; (b). when R 4 is 6-methyl, G is not N(methyl) 2 ; and (c) when R 4 is 6-F, G is not N-pyrrolidinyl. 2. The compound or pharmaceutically acceptable salt thereof according to claim 1, 15 wherein n is 1 or 2, and each R 4 is independently selected from halogen, alkyl, alkenyl, optionally substituted amino, hydroxyl, -0-alkyl, -S-alkyl, nitro and cyano. 3. The compound or pharmaceutically acceptable salt thereof according to claim 1 or 2, wherein E is S; and G is selected from an optionally substituted heteroaromatic group, an 20 optionally substituted aromatic group, and an optionally substituted alkyl group, and NR 2 R 3 , 67 wherein R 2 and R 3 may be the same or different and are individually selected from hydrogen, alkyl, alkenyl, and an optionally substituted aromatic group. 4. The compound or pharmaceutically acceptable salt thereof according to claim 1 or 2, 5 wherein E is S; and G is NR 2 R 3 , wherein R 2 and R 3 may be the same or different and are individually selected from hydrogen, alkyl, alkenyl, and an optionally substituted aromatic group.

5. The compound or pharmaceutically acceptable salt thereof according to claim 1 or 2, wherein E is 0; and G is selected from an optionally substituted 5- or 6-membered 10 heteroaromatic group, an optionally substituted aromatic group, an optionally substituted alkyl group, an optionally substituted heterocycloalkyl group, and NR 2 R 3 , wherein R 2 and R 3 may be the same or different and are individually selected from hydrogen, alkyl, alkenyl, and an optionally substituted aromatic group. 15

6. The compound or pharmaceutically acceptable salt thereof according to any one of claims 1 to 5, wherein each of said optional substituents is independently selected from C 1 - alkyl, C 2 -6 alkenyl, C 2 - 6 alkynyl, C 6 . 10 aryl, halo, amino, hydroxyl, 0-alkyl, S-alkyl, nitro, cyano, C(O) alkyl, C(O)-O-(Cl.6 alkyl), C(O)NH(C1.6 alkyl), and C(O)N(C1.6 alkyl) 2 . 20

7. The compound according to any one of claims 1 and 3 to 6, wherein said compound is a compound of Formula 4: N -N E - N /N G or a pharmaceutically acceptable salt thereof wherein 25 EisOorS;and G is as defined in claim 1, including provisos (i) and (ii).

8. A compound selected from 68 N N N N -N ' N NN I H HN H N -N HN -N S and S or a pharmaceutically acceptable salt thereof. 5

9. A metal ion complex of a compound according to any one of claims 1 to 8 with or without provisos (i)-(iii), or a pharmaceutically acceptable salt thereof, wherein the metal ion is selected from iron, copper, zinc, palladium, platinum, and gallium.

10 10. The metal ion complex or salt thereof according to claim 9, wherein the metal ion is selected from iron(lll) and iron(II).

11. A pharmaceutical composition comprising at least one compound according to any one of claims 1 to 8, or a metal ion complex or salt thereof according to claim 9 or 10, together 15 with a pharmaceutically acceptable diluent, adjuvant or excipient.

12. A method of iron chelation therapy or treating an iron-overload disorder in a mammal, the method comprising administering to said mammal a therapeutically effective amount of at least one compound or pharmaceutically acceptable salt thereof according to any 20 one of claims 1 to 8 with or without provisos (i)-(iii), or a pharmaceutical composition comprising at least one compound or pharmaceutically acceptable salt thereof according to any one of claims 1 to 8 with or without provisos (i)-(iii), together with a pharmaceutically acceptable diluent, adjuvant or excipient. 25

13. The method according to claim 12, wherein the iron overload disorder is p-thalassaemia. 69

14. A method of inhibiting cellular proliferation, or inducing apoptosis in a cell, or inhibiting cellular proliferation and inducing apoptosis in a cell, the method comprising contacting the cell with an effective amount of at least one compound or salt thereof according to any one of 5 claims 1 to 8 with or without provisos (i)-(iii), or a metal ion complex or pharmaceutically acceptable salt thereof according to claim 9 or 10, or a pharmaceutical composition according to claim 11, with the exclusion of the compound bis(2-pyridyl)ketone thiosemicarbazone.

15. A method of treating a proliferative disorder in a mammal, the method comprising 10 administering to said mammal a therapeutically effective amount of at least one compound or salt thereof according to any one of claims 1 to 8 with or without provisos (i)-(iii), or a metal ion complex or pharmaceutically acceptable salt thereof according to claim 9 or 10, or a pharmaceutical composition according to claim 11, with the exclusion of the compound bis(2 pyridyl)ketone thiosemicarbazone. 15

16. The method according to claim 15, wherein the proliferative disorder is selected from angiogenesis-dependent diseases, cellular proliferative diseases, inflammatory disorders, auto-immune diseases, blood vessel diseases, thrombosis and cancer. 20

17. The method according to claim 16, wherein the proliferative disorder is cancer.

18. The method according to claim 17, wherein the cancer is a solid tumour or a non-solid tumour. 25

19. The method according to any one of claims 12 to 18, wherein the compound is selected from: di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone; di-2-pyridylketone 4-methyl-3-thiosemicarbazone; di-2-pyridylketone 4-ethyl-3-thiosemicarbazone; 30 di-2-pyridylketone 4-allyl-3-thiosemicarbazone; di-2-pyridylketone 4-phenyl-3-thiosemicarbazone; di-2-pyridylketone isonicotinoyl hydrazone; di-2-pyridylketone benzoyl hydrazone; di-2-pyridylketone 4-hydroxybenzoyl hydrazone; 70 di-2-pyridylketone 3-bromobenzoyl hydrazone; di-2-pyridylketone 4-aminobenzoyl hydrazone; di-2-pyridylketone 2-thiophenecarboxaldehyde hydrazone; di-2-pyridylketone 4,4-diphenylcarboxaldehyde hydrazone; 5 d i-2-pyridylketone octanoic hydrazone; di-2-pyridylketone isonicotinyl thiohydrazone; di-2-pyridylketone benzoyl thiohydrazone; di-2-pyridylketone 4-hydroxybenzoyl thiohydrazone; di-2-pyridylketone 3-bromobenzoyl thiohydrazone; 10 di-2-pyridylketone 4-aminobenzoyl thiohydrazone; di-2-pyridylketone 2-thiophenecarboxaldehyde thiohydrazone; di-2-pyridylketone 4,4-diphenylcarboxaldehyde thiohydrazone; and di-2-pyridylketone octanoic thiohydrazone; or a pharmaceutically acceptable salt thereof. 15