AU2003223928A1 - Homologous recombination into bacterium for the generation of polynucleotide libraries - Google Patents

Homologous recombination into bacterium for the generation of polynucleotide libraries

Info

Publication number
AU2003223928A1
AU2003223928A1 AU2003223928A AU2003223928A AU2003223928A1 AU 2003223928 A1 AU2003223928 A1 AU 2003223928A1 AU 2003223928 A AU2003223928 A AU 2003223928A AU 2003223928 A AU2003223928 A AU 2003223928A AU 2003223928 A1 AU2003223928 A1 AU 2003223928A1
Authority
AU
Australia
Prior art keywords
bacterium
generation
homologous recombination
polynucleotide libraries
libraries
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003223928A
Inventor
Mads Eskelund Bjornvad
Peter Kamp Hansen
Per Lina Jorgensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of AU2003223928A1 publication Critical patent/AU2003223928A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2003223928A 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries Abandoned AU2003223928A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200200682 2002-05-07
DKPA200200682 2002-05-07
PCT/DK2003/000301 WO2003095658A1 (en) 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries

Publications (1)

Publication Number Publication Date
AU2003223928A1 true AU2003223928A1 (en) 2003-11-11

Family

ID=29414623

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003223928A Abandoned AU2003223928A1 (en) 2002-05-07 2003-05-07 Homologous recombination into bacterium for the generation of polynucleotide libraries

Country Status (2)

Country Link
AU (1) AU2003223928A1 (en)
WO (1) WO2003095658A1 (en)

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CN108165516A (en) * 2018-03-22 2018-06-15 江南大学 A kind of method of fermentation of bacillus subtilis production propylhomoserin dehydrogenase

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US20080038780A1 (en) * 2006-02-15 2008-02-14 Novozymes Biopolymer A/S Production of low molecular weight hyaluronic acid
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WO2011104339A1 (en) 2010-02-25 2011-09-01 Novozymes A/S Variants of a lysozyme and polynucleotides encoding same
WO2011109524A2 (en) 2010-03-03 2011-09-09 Novozymes, Inc. Xylanase variants and polynucleotides encoding same
BR112012025858A2 (en) 2010-04-09 2015-10-06 Eviagenics S A gene mosaic generation method.
WO2012035103A1 (en) 2010-09-16 2012-03-22 Novozymes A/S Lysozymes
WO2013026796A1 (en) 2011-08-19 2013-02-28 Novozymes A/S Polypeptides having protease activity
EP2766488A1 (en) 2011-10-12 2014-08-20 Eviagenics S.A. Method of generating gene mosaics in eukaryotic cells
AU2012360992B2 (en) 2011-12-28 2018-08-09 Novozymes A/S Polypeptides having protease activity
AR090971A1 (en) 2012-05-07 2014-12-17 Novozymes As POLYPEPTIDES THAT HAVE XANTANE DEGRADATION ACTIVITY AND POLYCINOCYLODES THAT CODE THEM
BR112014031882A2 (en) 2012-06-20 2017-08-01 Novozymes As use of an isolated polypeptide, polypeptide, composition, isolated polynucleotide, nucleic acid construct or expression vector, recombinant expression host cell, methods for producing a polypeptide, for enhancing the nutritional value of an animal feed, and for the treatment of protein, use of at least one polypeptide, animal feed additive, animal feed, and detergent composition
EP2934177B1 (en) 2012-12-21 2017-10-25 Novozymes A/S Polypeptides having protease activiy and polynucleotides encoding same
US11130969B2 (en) 2013-07-12 2021-09-28 Novozymes, A/S Direct transfer of polynucleotides between genomes
EP3453757B1 (en) 2013-12-20 2020-06-17 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
MX383142B (en) 2014-12-19 2025-03-13 Novozymes As COMPOSITIONS COMPRISING POLYPEPTIDES HAVING XYLANASE ACTIVITY AND POLYPEPTIDES HAVING ARABINOFURANOSIDASE ACTIVITY.
WO2016196202A1 (en) 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
US10450539B2 (en) 2015-06-04 2019-10-22 Novozymes A/S Use of M4 metalloprotease in wort production
EP3359657B1 (en) 2015-10-07 2020-04-01 Novozymes A/S Polypeptides
EP3362558A1 (en) 2015-10-14 2018-08-22 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
WO2017089366A1 (en) 2015-11-24 2017-06-01 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
MX2018008051A (en) 2016-01-29 2018-08-23 Novozymes As Beta-glucanase variants and polynucleotides encoding same.
US10808268B2 (en) 2016-05-24 2020-10-20 Novozymes A/S Polypeptides having alpha-galactosidase activity and polynucleotides encoding same
BR112018073890A2 (en) 2016-05-24 2019-02-26 Novozymes As composition, granule, animal feed additive, animal feed, liquid formulation, use of composition, granule, animal feed additive or liquid formulation, isolated polypeptide, methods for releasing galactose from plant-based material to improve one or more performance parameters of an animal and to produce the polypeptide, polynucleotide, nucleic acid construct or expression vector, and recombinant host cell.
CN109196098A (en) 2016-05-24 2019-01-11 诺维信公司 Composition comprising the polypeptide with galactanase activity and the polypeptide with betagalactosidase activity
CA3031609A1 (en) 2016-08-24 2018-03-01 Novozymes A/S Gh9 endoglucanase variants and polynucleotides encoding same
WO2018037065A1 (en) 2016-08-24 2018-03-01 Henkel Ag & Co. Kgaa Detergent composition comprising gh9 endoglucanase variants i
CN110072997A (en) 2016-11-23 2019-07-30 诺维信公司 Polypeptide with proteinase activity and the polynucleotides for encoding it
US11149233B2 (en) 2017-03-31 2021-10-19 Novozymes A/S Polypeptides having RNase activity
WO2018177936A1 (en) 2017-03-31 2018-10-04 Novozymes A/S Polypeptides having dnase activity
US11624059B2 (en) 2017-08-24 2023-04-11 Henkel Ag & Co. Kgaa Detergent compositions comprising GH9 endoglucanase variants II
CA3070749A1 (en) 2017-08-24 2019-02-28 Novozymes A/S Gh9 endoglucanase variants and polynucleotides encoding same
EP4501130A3 (en) 2017-09-01 2025-04-23 Novozymes A/S Animal feed additives comprising a polypeptide having protease activity and uses thereof
EP3684899A1 (en) 2017-09-22 2020-07-29 Novozymes A/S Novel polypeptides
CN111373036A (en) 2017-10-02 2020-07-03 诺维信公司 Polypeptides having mannanase activity and polynucleotides encoding same
US11746310B2 (en) 2017-10-02 2023-09-05 Novozymes A/S Polypeptides having mannanase activity and polynucleotides encoding same
CN111148830A (en) 2017-10-04 2020-05-12 诺维信公司 Polypeptides with protease activity and polynucleotides encoding the same
WO2019081515A1 (en) 2017-10-24 2019-05-02 Novozymes A/S Compositions comprising polypeptides having mannanase activity
WO2019096903A1 (en) 2017-11-20 2019-05-23 Novozymes A/S New galactanases (ec 3.2.1.89) for use in soy processing
US20210102184A1 (en) 2018-02-23 2021-04-08 Henkel Ag & Co. Kgaa Detergent composition comprising xanthan lyase and endoglucanase variants
US11473076B2 (en) 2018-08-31 2022-10-18 Novozymes A/S Animal feed additives and compositions comprising an S8 serine protease
WO2020070199A1 (en) 2018-10-03 2020-04-09 Novozymes A/S Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same
WO2020201403A1 (en) 2019-04-03 2020-10-08 Novozymes A/S Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions
US20220411773A1 (en) 2019-12-20 2022-12-29 Novozymes A/S Polypeptides having proteolytic activity and use thereof
US20230235367A1 (en) 2020-02-10 2023-07-27 Novozymes A/S Process for producing ethanol from raw starch using alpha-amylase variants
EP4103709A2 (en) 2020-02-10 2022-12-21 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
US20230142991A1 (en) 2020-02-10 2023-05-11 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2022161914A1 (en) 2021-01-26 2022-08-04 Basf Se High temperature fermentation process and microorganisms
EP4451889A1 (en) 2021-12-22 2024-10-30 Novozymes A/S Method of producing a milk-based product
EP4242303A1 (en) 2022-03-08 2023-09-13 Novozymes A/S Fusion polypeptides with deamidase inhibitor and deamidase domains
US20250215412A1 (en) 2022-03-08 2025-07-03 Novozymes A/S Fusion Polypeptides with Deamidase Inhibitor and Deamidase Domains
WO2024121357A1 (en) 2022-12-08 2024-06-13 Novozymes A/S Fiber-degrading enzymes for animal feed comprising an oil seed material
CN120476196A (en) 2022-12-21 2025-08-12 诺维信公司 Microbial proteases for cell separation
EP4389886A1 (en) 2022-12-22 2024-06-26 Novozymes A/S Lactase enzymes and method of producing a milk-based product
WO2025196175A1 (en) 2024-03-22 2025-09-25 Novozymes A/S Oral care compositions comprising dnases

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AU2001265835A1 (en) * 2000-06-23 2002-01-08 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
WO2002014490A2 (en) * 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108165516A (en) * 2018-03-22 2018-06-15 江南大学 A kind of method of fermentation of bacillus subtilis production propylhomoserin dehydrogenase
CN108165516B (en) * 2018-03-22 2020-07-07 江南大学 A kind of method of Bacillus subtilis fermentation to produce leucine dehydrogenase

Also Published As

Publication number Publication date
WO2003095658A1 (en) 2003-11-20

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Legal Events

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MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase
TH Corrigenda

Free format text: IN VOL 18, NO 2, PAGE(S) 562 UNDER THE HEADING APPLICATIONS OPI - NAME INDEX UNDER THE NAME NOVOZYMES A/S, APPLICATION NO. 2003223928, UNDER INID (43) CORRECT THE PUBLICATION DATE TO READ 24.11.2003