AR083221A1 - PROCESS TO PRODUCE NUCLEOSID DIALYLPHOSPHOTRIESTERS THROUGH ENZYMATIC TRANSESTERIFICATION AND DEPROTECTION OF THE SAME TO PRODUCE MONOPHOSPHATE NUCLEOSIDS - Google Patents
PROCESS TO PRODUCE NUCLEOSID DIALYLPHOSPHOTRIESTERS THROUGH ENZYMATIC TRANSESTERIFICATION AND DEPROTECTION OF THE SAME TO PRODUCE MONOPHOSPHATE NUCLEOSIDSInfo
- Publication number
- AR083221A1 AR083221A1 ARP110103609A ARP110103609A AR083221A1 AR 083221 A1 AR083221 A1 AR 083221A1 AR P110103609 A ARP110103609 A AR P110103609A AR P110103609 A ARP110103609 A AR P110103609A AR 083221 A1 AR083221 A1 AR 083221A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleoside
- phosphotriester
- produce
- dialkyl
- monophosphate
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- 238000005809 transesterification reaction Methods 0.000 title abstract 2
- VZQXUWKZDSEQRR-UHFFFAOYSA-N Nucleosid Natural products C12=NC(SC)=NC(NCC=C(C)C)=C2N=CN1C1OC(CO)C(O)C1O VZQXUWKZDSEQRR-UHFFFAOYSA-N 0.000 title 1
- 238000010511 deprotection reaction Methods 0.000 title 1
- 150000004712 monophosphates Chemical class 0.000 title 1
- 239000002777 nucleoside Substances 0.000 abstract 9
- 150000003833 nucleoside derivatives Chemical class 0.000 abstract 6
- 102000004203 Phosphoric Triester Hydrolases Human genes 0.000 abstract 3
- 108090000754 Phosphoric Triester Hydrolases Proteins 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- -1 monophosphate nucleoside Chemical class 0.000 abstract 3
- 229910019142 PO4 Inorganic materials 0.000 abstract 2
- 125000000217 alkyl group Chemical group 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 2
- 239000010452 phosphate Substances 0.000 abstract 2
- 102000004861 Phosphoric Diester Hydrolases Human genes 0.000 abstract 1
- 108090001050 Phosphoric Diester Hydrolases Proteins 0.000 abstract 1
- 239000013043 chemical agent Substances 0.000 abstract 1
- 238000005984 hydrogenation reaction Methods 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 150000007522 mineralic acids Chemical class 0.000 abstract 1
- 150000007524 organic acids Chemical class 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/04—Phosphoric diester hydrolases (3.1.4)
- C12Y301/04001—Phosphodiesterase I (3.1.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/08—Phosphoric triester hydrolases (3.1.8)
- C12Y301/08001—Aryldialkylphosphatase (3.1.8.1), i.e. paraoxonase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Reivindicación 1: Un proceso para producir un dialquilfosfotriéster de nucleósido, donde el proceso comprende la reacción de un nucleósido con un compuesto fosfotriéster activado como un dador de fosfato en presencia de una enzima fosfotriesterasa.Reivindicación 12: Un proceso para producir un nucleósido monofosfato monoéster, el proceso que comprende las etapas de: a) conducir la transesterificación enzimática de acuerdo con la reivindicación 1, mediante la reacción de un nucleósido con un compuesto fosfotriéster activado como un dador de fosfato en presencia de una enzima fosfotriesterasa, b) eliminar los grupos alquilo del dialquilfosfotriéster de nucleósido obtenido en la etapa (a) mediante reacción con un agente químico seleccionado del grupo de reactivos básicos inorgánicos u orgánicos, reactivos ácidos inorgánicos u orgánicos, tioalcoholes y reactivos de hidrogenación, o c) eliminar los grupos alquilo del dialquilfosfotriéster de nucleósido obtenido en la etapa (a) mediante hidrólisis de acuerdo con las siguientes etapas: i) incubar el dialquilfosfotriéster de nucleósido en presencia de una enzima fosfotriesterasa, ii) incubar el alquilfosfodiéster de nucleósido de la etapa (i) en presencia de una enzima fosfodiesterasa, y recoger el nucleósido monofosfato de la mezcla de reacción.Claim 1: A process for producing a nucleoside dialkyl phosphotriester, wherein the process comprises the reaction of a nucleoside with an activated phosphotriester compound as a phosphate donor in the presence of a phosphotriesterase enzyme. Claim 12: A process for producing a monoester monophosphate nucleoside, the process comprising the steps of: a) conducting the enzymatic transesterification according to claim 1, by reacting a nucleoside with an activated phosphotriester compound as a phosphate donor in the presence of a phosphotriesterase enzyme, b) eliminating the alkyl groups of the nucleoside dialkyl phosphotriester obtained in step (a) by reaction with a chemical agent selected from the group of basic inorganic or organic reagents, inorganic or organic acid reagents, thioalcohols and hydrogenation reagents, or c) eliminating the alkyl groups of the nucleoside dialkyl phosphotriester obtained and n step (a) by hydrolysis according to the following steps: i) incubating the nucleoside dialkyl phosphotriester in the presence of a phosphotriesterase enzyme, ii) incubating the nucleoside alkyl phosphodiester of step (i) in the presence of a phosphodiesterase enzyme, and collect the nucleoside monophosphate from the reaction mixture.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38767010P | 2010-09-29 | 2010-09-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR083221A1 true AR083221A1 (en) | 2013-02-06 |
Family
ID=44773066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP110103609A AR083221A1 (en) | 2010-09-29 | 2011-09-29 | PROCESS TO PRODUCE NUCLEOSID DIALYLPHOSPHOTRIESTERS THROUGH ENZYMATIC TRANSESTERIFICATION AND DEPROTECTION OF THE SAME TO PRODUCE MONOPHOSPHATE NUCLEOSIDS |
Country Status (2)
| Country | Link |
|---|---|
| AR (1) | AR083221A1 (en) |
| WO (1) | WO2012041965A1 (en) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA117095C2 (en) | 2011-12-22 | 2018-06-25 | Аліос Біофарма, Інк. | NUCLEOSIDE COMPOUND OR PHARMACEUTICALALLY SUITABLE SALT |
| USRE48171E1 (en) | 2012-03-21 | 2020-08-25 | Janssen Biopharma, Inc. | Substituted nucleosides, nucleotides and analogs thereof |
| US9441007B2 (en) | 2012-03-21 | 2016-09-13 | Alios Biopharma, Inc. | Substituted nucleosides, nucleotides and analogs thereof |
| SG11201407336PA (en) | 2012-05-25 | 2015-03-30 | Janssen Sciences Ireland Uc | Uracyl spirooxetane nucleosides |
| GEP201706757B (en) | 2012-12-21 | 2017-10-25 | Alios Biopharma Inc | Substituted nucleosides, nucleotides and analogs thereof |
| AU2014250762A1 (en) | 2013-04-12 | 2015-10-29 | Achillion Pharmaceuticals, Inc. | Highly active nucleoside derivative for the treatment of HCV |
| EP3055319A4 (en) | 2013-10-11 | 2018-01-10 | Alios Biopharma, Inc. | Substituted nucleosides, nucleotides and analogs thereof |
| CA3182565A1 (en) | 2015-03-06 | 2016-09-15 | Atea Pharmaceuticals, Inc. | .beta.-d-2'-deoxy-2'-.alpha.-fluoro-2'-.beta.-c-substituted-2-modified-n6-substituted purine nucleotides for hcv treatment |
| EA201890454A1 (en) | 2015-08-06 | 2018-07-31 | Чимерикс, Инк. | PYRROPHYRIMIDINE NUCLEOSIDE AND THEIR ANALOGUES THAT CAN BE USED AS ANTI-VIRUS MEANS |
| DK3512863T3 (en) | 2016-09-07 | 2022-03-07 | Atea Pharmaceuticals Inc | 2β-substituted-N6-substituted purine nucleotides for the treatment of RNA virus |
| CN115477679A (en) | 2017-02-01 | 2022-12-16 | 阿堤亚制药公司 | Nucleotide hemisulfates for the treatment of hepatitis C virus |
| WO2019060692A1 (en) | 2017-09-21 | 2019-03-28 | Chimerix, Inc. | MORPHIC FORMS OF 4-AMINO-7-(3,4-DIHYDROXY-5-(HYDROXYMETHYL)TETRAHYDROFURAN-2-YL)-2-METHYL-7H-PYRROLO[2,3-d]PYRIMIDINE-5-CARBOXAMIDE AND USES THEREOF |
| EP3773753A4 (en) | 2018-04-10 | 2021-12-22 | ATEA Pharmaceuticals, Inc. | Treatment of hcv infected patients with cirrhosis |
| US10874687B1 (en) | 2020-02-27 | 2020-12-29 | Atea Pharmaceuticals, Inc. | Highly active compounds against COVID-19 |
| KR20240022574A (en) | 2021-06-17 | 2024-02-20 | 아테아 파마슈티컬즈, 인크. | Favorable anti-HCV combination therapy |
| TW202400134A (en) | 2022-03-15 | 2024-01-01 | 美商羅米醫療公司 | Compounds and methods for treating disease |
| WO2024086592A2 (en) * | 2022-10-17 | 2024-04-25 | Emory Unversity | 4'-halogen containing nucleotide and nucleoside therapeutic compositions and uses related thereto |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5756315A (en) | 1989-12-05 | 1998-05-26 | Kyowa Hakko Kogyo Co., Ltd. | Inosine-guanosine kinase |
| US6987008B1 (en) | 1999-09-03 | 2006-01-17 | Ajinomoto Co., Inc. | Variant nucleoside-5′-phosphate producing enzyme |
| AUPR502301A0 (en) * | 2001-05-15 | 2001-06-07 | Commonwealth Scientific And Industrial Research Organisation | Phosphotriesterase from Agrobacterium radiobacter P230 |
-
2011
- 2011-09-29 AR ARP110103609A patent/AR083221A1/en unknown
- 2011-09-29 WO PCT/EP2011/066988 patent/WO2012041965A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012041965A1 (en) | 2012-04-05 |
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