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All Journal HAYATI Journal of Biosciences Biosel: Biologi Science and Education Ininnawa: Jurnal Pengabdian Masyarakat Bionature
Musawira
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Public Health Social Sciences

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Drought Tolerance in Transgenic Potato Cultivar IPB CP1 Expressing MmCuZn-SOD Gene Musawira; Tjahjoleksono, Aris; Hamim, Hamim; Miftahudin, M.
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1377-1386

Abstract

Drought stress induces reactive oxygen species (ROS) accumulation, which is harmful to plant cells. Consequently, it inhibits plant growth and decreases yield. The enzyme superoxide dismutase (SOD), which is encoded by the SOD gene, is the first defence enzyme in the cells that detoxify ROS. The study aimed to analyze the tolerance of transgenic potato cultivar IPB CP1 and its yield under drought-stress conditions. The results showed that transgenic plants had superior morphological characteristics, such as plant height, number of leaves, stem diameter, and plant biomass, than non-transgenic plants. However, photosynthetic rate, chlorophyll content, stomatal conductance, and transpiration rate showed similar levels between the transgenic and non-transgenic plants. The transgenic plants expressing the MmCu/Zn-SOD gene showed lower lipid peroxidation levels than the non-transgenic plants, indicating that the gene works well to reduce the cell's ROS level. Transgenic plant clone CP1S6 showed 13 times higher gene expression and tuber yield than non-transgenic plants. These research indicated that the transgenic plants expressing the MmCu/Zn-SOD gene are more tolerant to drought stress than the non-transgenic plants.
Pelatihan Analisis Data Kuantitatif sebagai Strategi Peningkatan Keterampilan Riset ilmiah Mahasiswa Patongai, Dian Dwi Putri Ulan Sari; Junda, Muhammad; Sartika, Dewi; Musawira; Anggita, Sri Ayu
Ininnawa : Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2025): Vol. 3 No. 1 (2025): Volume 03 Nomor 01 (April 2025)
Publisher : Program Studi Manajemen FEB UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/ininnawa.v3i1.8559

Abstract

The ability of students to perform quantitative data analysis is a crucial skill in conducting scientific research. However, many students face challenges, both in selecting appropriate analytical techniques relevant to their research topics and in using data analysis software such as SPSS or other supporting applications. These challenges often hinder students in analyzing research data effectively. This community service activity aims to enhance students' skills in identifying suitable analytical techniques for scientific research and in using data analysis software such as SPSS. The implementation methods include delivering materials on descriptive and inferential statistics, conducting simulations on SPSS usage, and facilitating discussion and Q&A sessions. The results of the training show an improvement in participants’ understanding of how to choose appropriate analytical techniques and operate statistical software for research purposes. This activity also contributes to increasing students’ confidence and independence in conducting scientific research. The training is expected to serve as a sustainable and integrated model for academic development in higher education institutions.
Antibacterial Activity Test of Matoa (Pometia pinnata) Leaves Ethanol Extract Against Salmonella typhi and Bacillus subtilis Pagarra, Halifah; Taiyeb , Mushawwir; Junda , Muhammad; Musawira; Muhammad Naufal Syaiful Haq; Handayani , Resti
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7234

Abstract

This study aims to determine the antimicrobial activity of ethanol extract of matoa leaves (Pometia pinnata) against Salmonella typhi and Bacillus subtilis bacteria. This study began with preparing raw materials in the form of matoa leaves and then extracting the matoa leaves using the maceration method with 70% solvent. After that, the antimicrobial activity test was carried out using the disc diffusion method with four concentration variations, namely 5%, 7.5%, 10%, and 12.5% with a positive control in the form of 50 ppm amoxicillin and a negative control using distilled water, each treatment using three replications. The results in the form of inhibition zones formed were measured using calipers. The data obtained were then analyzed using SPSS 30 with the ANOVA test and further tests in the form of tests. The test results showed that 70% ethanol extract of matoa leaves had an effect on inhibiting the growth of Salmonella typhi and Bacillus subtilis bacteria. The largest inhibition zone in Salmonella typhi was found at a concentration of 7.5% with a diameter of 16.87 mm, while in Bacillus subtilis it was found at a concentration of 12.5% with a diameter of 12.22 mm. Keywords: Pometia pinnata, Antimicrobial activity, Salmonella typhi, Bacillus subtilis
The English Halifah Pagarra; Rachmawaty; Musawira; Handayani, Besti; Syaiful Haq, Muhammad Naufal
Biosel Biology Science and Education Vol. 14 No. 2 (2025): BIOSEL (Biology Science and Education: Jurnal Penelitian Science dan Pendidika
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v14i2.9376

Abstract

Matoa (Pometia pinnata) is a plant commonly used by the Indonesian people for traditional medicine due to its rich content of metabolites. This study aimed to analyze the phytochemical composition and antibacterial activity of matoa leaves The leaves were extracted using a 70% ethanol solvent with a ratio of 1:4 using the maceration method, and phytochemical screening was performed on the leaf extract. The antibacterial activity was tested against Staphylococcus aureus and Escherichia coli using the disc diffusion method. The results indicated that the matoa leaf extract contains various metabolites, including triterpenoids, steroids, flavonoids, phenolics, saponins, tannins, and alkaloids. Secondary metabolite compounds in matoa leaves are thought to be able to inhibit microbial growth so that inhibition zones can be formed. Furthermore, the antibacterial activity demonstrated that the matoa leaf extract effectively inhibited the growth of S. aureus and E. coli. the largest inhibition zone is of 18.22 mm at 10% extract concentration, and E. coli, with an inhibition zone largest is of 10.87 mm at 10% extract concentration. While the smallest inhibition zone in S. aureus is 15.29 mm at 7.5% concentration, and in E. coli is 5% at 5% extract concentration.. This study suggests that matoa leaves have the potential to serve as natural bioactive compounds and antimicrobial agents in the clinical industry. Keywords: Matoa, Phytochemical,  Antibacterial activity, Staphylococcus aureus, Escherichia coli
Co-Authors Anggita, Sri Ayu Aris Tjahjoleksono DEWI SARTIKA Halifah Pagarra Halifah Pagarra Hamim Hamim Handayani , Resti Handayani, Besti Junda , Muhammad Miftahudin, M. Muhammad Junda Muhammad Naufal Syaiful Haq Patongai, Dian Dwi Putri Ulan Sari Rachmawaty Syaiful Haq, Muhammad Naufal Taiyeb , Mushawwir