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Tingkat Pengetahuan Masyarakat Tentang Potensi Bunga Rosella (Hibiscus sabdariffa L.) Sebagai Herbal Medicine di Kenten Laut Sumatera Selatan: Tingkat Pengetahuan Masyarakat Tentang Potensi Bunga Rosella (Hibiscus sabdariffa L.) Sebagai Herbal Medicine di Kenten Laut Sumatera Selatan Kaswijan, Siti Rohani; Trisnawati; Astri, Yesi; Zilda, Malika; Melianti, Agnes
DHARMA RAFLESIA Vol 22 No 2 (2024): DESEMBER (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v22i2.35000

Abstract

Pemanfaatan rosella sebagai herbal medicine telah banyak dilakukan dalam beberapa dekade ini. Penggunaan rosella telah diteliti sebagai antioksidan, antibakteri, dan lainnya. Pemahaman masyarakat dalam penggunaan rosella sebagai herbal medicine perlu dilakukan sebagai tindakan mengedukasi potensi dan peluang budidaya rosella, salah satunya melalui sosialisasi. Sosialisasi telah dilakukan di desa Kenten Laut, Sumatera Selatan terkait potensi, pengolahan, pemanfaatan dan pengembangan rosella sebagai herbal medicine. Hasil kegiatan dievaluasi dari responden terhadap capaian nilai berdasarkan pretest dan posttest. Menggunakan pendekatan statistik, didapatkan peningkatan nilai pemahaman responden tentang rosella sebagai herbal medicine. Hasil analisis didapatkan 25 orang responden, sebanyak 64% memiliki nilai posttest lebih besar daripada pretest. Hasil uji wilcoxon signed rank test dari nilai negative ranks menunjukkan perbedaan hasil setelah dilakukan penyuluhan dengan nilai asymp.sig. (2-tailed) pada angka 0,001. Nilai Zscore dari wilcoxon signed rank test menunjukan nilai Z (-3,300) < Ztabel(60) (P=0,05), terdapat korelasi peningkatan pengetahuan responden setelah sosialisasi. Sosialisasi lanjutan diperlukan untuk membangun kapabilitas masyarakat dalam pemanfaatan rosella sebagai herbal medicine pada usaha mikro, kecil dan menengah (UMKM) secara komersial.
Spectroscopic characterization of rosella flower extract (Hibiscus sabdariffa L.) and its antibacterial activity against Enterobacter aerogenes in suspected typhoid cases Rohani, Siti; Martha, Fadhil Rizki; Mundijo, Trisnawati; Yesi Astri; Zilda, Malika; Melianti, Agnes
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.5577

Abstract

Typhoid, a serious bacterial disease, has spurred research into natural products like rosella (Hibiscus sabdariffa L.) for potential treatments. This study investigates the chemical components found in Rosella extract using spectroscopy approximation, assisted by Fourier transform infrared spectroscopy (FTIR) and Gas chromatography–mass spectrometry (GC–MS). The antibacterial activity of rosella extract on bacteria from stool cultures of suspected typhoid cases continues to be evaluated. The antibacterial experimental employed a post-test-only control group design, using 30 μg Chloramphenicol as a positive control, sterile distilled water as the negative control, rosella extract at concentrations of 25%, 50%, 75%, and 100% as the observed variable. Stool samples from typhoid patients were identified, and Enterobacter aerogenes were detected using VITEK®2 testing. Cultivated bacteria from the samples were tested to determine the antibacterial activity of the rosella extract. Phytochemical studies confirmed the presence of tannins, alkaloids, flavonoids, and saponins in the rosella extract. Additionally, the spectroscopic evaluation from FTIR and GC-MS showed the presence of chemical groups, including esters, aldehydes, and aromatics. Further clinical tests demonstrated antibacterial activity at the minimum inhibitory concentration. The results showed an increasing inhibition zone of bacterial growth, correlating with the increase in rosella extract concentration. Although the antibacterial activity of rosella extract was lower compared to commercial Chloramphenicol, this natural product has demonstrated antibacterial activity and shows potential as a candidate for future herbal medicine development.