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All Journal Molekul: Jurnal Ilmiah Kimia
Muslihah, Niken Istikhari
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Immobilization of Urease from Psophocarpus tetragonolobus L. DC. using Natrium Alginate Supporting Matrix Zusfahair, Zusfahair; Ningsih, Dian Riana; Lestari, Puji; Bilalodin, Bilalodin; Aryanti, Eva; Muslihah, Niken Istikhari
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.7335

Abstract

Urease is an enzyme that has the role to hydrolyzes urea into ammonia and carbon dioxide. Immobilization is one of the most efficient strategies to improve its activity recovery and properties of urease. This research started with the germination of winged beans for 8 days. The winged bean was extracted by grinding using a mortar and pestle and then added with phosphate buffer at pH 7. The solution was homogenized using a stirrer and then centrifuged in cold conditions so that an extract of urease was obtained. Urease extracts were immobilized using a chitosan-supporting matrix. Optimization of the immobilization process of urease extract includes the concentration of chitosan and sodium tripolyphosphate (TPP) and contact time. The obtained was free and immobilized urease activities then tested using the Nessler method and measured using a UV-Vis spectrophotometer with a wavelength of 500 nm. The obtained data were then statistically tested using ANOVA. Urease-chitosan beads were further tested in repeated use and analyzed with SEM-EDX (Scanning Electron Microscopy-Energy Dispersive X-ray). The results showed that the optimum conditions for making urease-chitosan beads were a concentration of 4% (w/v), 2.5% (w/v) TPP, and 60 minutes of contact time, resulting in an activity value of 15.076 U/mL, which can be used 5 times with 46% activity from the initial activity. The EDX analysis results after the addition of the enzyme showed atom composition changes leading to increasing carbon and nitrogen contents. The existence of phosphor showed that TPP was a chitosan cross-link compound. Keywords: Chitosan, immobilization, TPP, urease, winged bean
Optimization and Characterization of Urease Immobilization from Red Lentil Seeds (Lens culinaris) Using Chitosan zusfahair, zusfahair; Ningsih, Dian Riana; Bilalodin, Bilalodin; Fatoni, Amin; Luthfia, Adilla; Purwati, Purwati; Muslihah, Niken Istikhari; Apriliadina, Inessa Putri
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13134

Abstract

ABSTRACT. Urease is an enzyme that plays a vital role in catalyzing the hydrolysis of urea into ammonia (NH3) and carbon dioxide (CO2). This study focuses on the isolation of urease from red lentil seeds, followed by its immobilization. The objective of this research is to optimize and characterize urease that has been immobilized using chitosan and activated with glutaraldehyde. Red lentil seeds were processed with a mortar and pestle at low temperatures (4 °C) to obtain a crude enzyme extract, which was then concentrated using 50% acetone (P50) prior to immobilization. The optimization process for P50 urease immobilization involved assessing various factors, including chitosan concentration, glutaraldehyde concentration, temperature, and the immersion duration in glutaraldehyde. The findings revealed that the optimal conditions for immobilizing P50 urease were achieved at a chitosan concentration of 0.75%, with a 2% glutaraldehyde soak at 25 °C for 2 hours, resulting in an enzyme activity of 7.042 U/g. The immobilized P50 urease demonstrated the ability to be reused up to 7 times while maintaining 51% of its initial activity. Scanning Electron Microscopy (SEM) analysis indicated morphological changes in the beads after the addition of glutaraldehyde and the enzyme, shifting from a rounded to an irregular shape. Additionally, Fourier Transform Infrared Spectroscopy (FTIR) analysis identified C-N and C=N peaks, confirming the successful incorporation of glutaraldehyde. Keywords: immobilization, red lentil seeds, glutaraldehyde, chitosan, urease
Co-Authors AMIN FATONI Apriliadina, Inessa Putri Aryanti, Eva Bilalodin Bilalodin Dian Riana Ningsih Luthfia, Adilla PUJI LESTARI Purwati Purwati Zusfahair Zusfahair