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Pratama, Chelsylia Dara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology

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Variasi Genetik Prion Protein Gene pada Kucing Domestik (Felis catus) Menggunakan Metode RFLP In Silico Pinta, Sari Rahma; Pratama, Chelsylia Dara; Fatiha, Fathma Dwi; Muharani, Silvia; Achyar, Afifatul
Jurnal Jeumpa Vol 11 No 1 (2024): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v11i1.9933

Abstract

FSE adalah penyakit neurodegeneratif yang disebabkan oleh prion yang mempengaruhi semua keluarga kucing. Penelitian ini bertujuan untuk mengidentifikasi variasi genetik dalam prion protein gene pada kucing domestik (Felis catus) menggunakan RFLP In silico. Penelitian ini merupakan penelitian deskriptif yang menyajikan karakteristik dari sampel yang diuji. Sampel yang digunakan yaitu sekuen prion protein gene dalam format fasta dari NCBI, dengan nomor identitas NCBI Popset 2445476754. Skrining enzim restriksi dilakukan menggunakan situs insilico.ehu.es. Metode RFLP in silico ini dilakukan menggunakan tools pada situs benchling.com dengan enzim restriksi yang diperoleh selama tahap skrining. Data yang telah didapatkan dianalisis secara deskriptif. Enzim restriksi yang digunakan yaitu enzim HhaI dengan sisi pemotongan pada basa G_CG'C dan enzim DpnI dengan sisi pemotongan pada GA'TC. Hasil restriksi dengan enzim HhaI pada 25 isolat prion protein gene Felis catus menghasilkan 4 variasi alel yaitu alel A1 (frekuensi alel 0,08), alel A2 (frekuensi alel 0,16), alel A3 (frekuensi alel 0,68) dan alel A4 (frekuensi alel 0,08). Restriksi yang dilakukan oleh enzim DpnI menghasilkan 2 alel yaitu alel B1(frekensi alel 0,84) dan alel B2 (frekuensi alel 0,16). Hasil penelitian ini dapat digunakan sebagai rujukan awal penelitian tentang gen Prion kucing domestik pada tahap yang lebih kompleks seperti bioinformatika, maupun pada tahap kerja laboratorium.
Correlation between Environmental Factors and Morphological Characteristics of Koenih Rimbo (Curcuma sumatrana Miq.) as an Endangered Plant in West Sumatra Jannah, Miftahul; Putri, Irma Leilani Eka; Kardiman, Reki; Anhar, Azwir; Putri, Dwi Hilda; Pratama, Chelsylia Dara; Valofi, Nagra Aulia; Putri, Cici Adelia; Aisyah, Siti; Djoelvinanda, Habibullah
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8604

Abstract

Curcuma sumatrana or Koenih Rimbo, is an endemic plant of West Sumatra that is threatened with extinction due to deforestation, climate change, and human activities. This study aims to understand the ecology of C. sumatrana to support effective conservation efforts. The study was conducted in seven potential locations in West Sumatra using exploration methods and analysis of environmental factors, such as altitude, temperature, humidity, soil pH, and canopy cover. The results showed that this species was only found in three locations, namely Lubuk Kilangan, Koto Malintang, and Koto Pulai, with the highest population in Koto Malintang. Environmental factors such as altitude, canopy cover, and air humidity were shown to support optimal growth of this species. Canonical Correspondence Analysis (CCA) identified a close relationship between environmental factors and morphological parameters, including plant height, number of branches, and leaf size. This study emphasizes the importance of natural habitat conservation and further research on the ecology of this species to prevent its extinction.
Co-Authors Achyar, Afifatul Azwir Anhar Djoelvinanda, Habibullah Dwi Hilda Putri Fatiha, Fathma Dwi Kardiman, Reki Miftahul Jannah Muharani, Silvia Pinta, Sari Rahma Putri, Cici Adelia Putri, Irma Leilani Eka Siti Aisyah Valofi, Nagra Aulia