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All Journal Animal Production : Indonesian Journal of Animal Production
T Laswardi Yusuf, T Laswardi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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The Viability of the Stallion Spermatozoa after Centrifugation with Different Level of Seminal Plasma in Skim Milk Extender Arifiantini, RI; Yusuf, T Laswardi; Purwantara, B
ANIMAL PRODUCTION Vol 8, No 3 (2006): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective of the research was to evaluate the speed and duration of centrifugation and seminal plasma levels on the viability of stallion spermatozoa.   Semen was collected from three stallions twice a week. The collected semen then evaluated macro- and microscopically.  Exp I, the semen was diluted 1:1 with skim milk extender and then centrifuged at 2000 and 3000 rpm for 15 and 20 minutes each. The seminal plasma was removed, and the sperm pellet was re-diluted with skim milk extender. The extended semen then divided in two tubes, stored in 5oC and in a room temperature.  The semen then was examined every 3 hours for room temperature and 12 hours for 5oC.  Exp II, the semen diluted 1:1 with skim milk extender and then centrifuged at 3000 rpm for 15 minutes. The seminal plasma was removed and the pellet was re-diluted with skim milk extender consisted of 0%, 25%, 50% and 75% of the seminal plasma.  The extended semen then was stored in 5oC and examined daily.  Results showed that speed and duration of centrifugation had no effect on the quality of stallion semen. The liquid semen without seminal plasma showed 47.50% motile sperms and 61.00% life sperms, wich were significantly higher (P<0.05) than those with seminal plasma. It was concluded that the speed and duration of centrifugation had no effect on the semen quality and removal of seminal plasma is beneficial for stallion spermatozoa. (Animal Production 8(3): 160-167 (2006) Key Words : Centrifugation, stallion semen and seminal plasma
The Effect of Glycerolisation Time on the Sperm Motility of Local Ram Frozen Semen Diluted in Tris Egg Yolk Extender Yusuf, T Laswardi; Arifiantini, RI; Mulyadi, Y
ANIMAL PRODUCTION Vol 8, No 3 (2006): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The aims of this experiment was to obtain the effects of  different time of glycerolisation on  the quality of frozen ram semen diluted in Tris Egg Yolk (TEY).  Semen from seven sexually mature local rams were collected using artificial vagina weekly, evaluated macroscopically and microscopically.  Good quality semen then diluted with TEY and 6% glycerol added in three different periods.  The first  step was  diluted in room temperature ( treatment 1), the second glycerolisation was at the end of  equilibration (treatment 2) and the third period was several steps of  glycerolisation during  equilibration (treatment 3). Diluted semen was  packed in straws (Minitub, Germany), equilibrated at 5oC for three  hours,  freezed in liquid nitrogen vapor for 10 minutes and then stored in a container of -196 oC liquid nitrogen.  After 24 hours, the semen was thawed at 37oC for 30 second.  The results of showed that there was no significant differences among treatments (P>0.05).  The percentage of post thawing motility was 42.50% (treatment 1), compared to 38.75% (treatment 2), and 30.00% (treatment 3).  In conclusion, the glycerolization  on frozen ram semen can be done any time of the  equilibration without influencing   the sperm motility. (Animal Production 8(3): 168-173 (2006) Key Words: Glycerolisation, equilibration, ram semen
Co-Authors B Purwantara RI Arifiantini, RI Y Mulyadi, Y