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All Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Journal of Forestry Research
Henti Rosdayanti, Henti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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MULTIPLIKASI TUNAS DAN INDUKSI UMBI MIKRO SATOIMO (Colocasia esculenta (L.) Schott) PADA BEBERAPA KONSENTRASI SUKROSA DAN BENZILAMINOPURIN Maretta, Delvi; Handayani, Dwi Pangesti; Rosdayanti, Henti; Tanjung, Armelia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 3, No 2 (2016): December 2016
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.75 KB) | DOI: 10.29122/jbbi.v3i2.150

Abstract

Taro or Satoimo (Colocasia esculenta (L) Schott var antiquorum) is an alternative of non-rice food. To support saitomo mass cultivation in several regions in Indonesia, shoot multiplication and induction of satoimo microtuber through in vitro technique is amongst the stage to be undertaken. The aims of this study were to determine the effect of BAP (benzylaminopurine) and sucrose for shoot multiplication and microtuber induction of in vitro culture of satoimo. The experiment was arranged in two factors: BAP (0, 1, 2 and 3 mg/L) and sucrose (30, 60, 90 and 120 g/L). The result showed that the single effect of BAP or sucrose and interaction of both significantly increased the number of shoots. The effect of 2 mg/L BAP was more homogeneous than that of 1 and 3 mg/L BAP. Sucrose with the concentration of 30 g/L was the best concentration for shoot multiplication. The highest number of microtuber was achieved with 2 mg/L BAP + 30 g/L sucrose treatments, but tended to decrease due to increasing sucrose concentration. In 2 and 3 mg/L BAP treatments, the number of microtuber increased along with the increasing sucrose concentration.Keywords: satoimo, in vitro shoot, microtuber, benzylaminopurine, sucrose ABSTRAKSatoimo (Colocasia esculenta (L) Schott var antiquorum) merupakan bahan pangan alternatif non-beras. Untuk mendukung produksi massal satoimo di beberapa wilayah di Indonesia, multiplikasi tunas dan induksi umbi mikro secara in vitro merupakan tahapan yang harus dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh BAP dan sukrosa terhadap multiplikasi tunas dan induksi umbi mikro satoimo dalam kultur in vitro. Perlakuan terdiri dari 4 taraf konsentrasi BAP (0, 1, 2 dan 3 mg/L) dan 4 taraf konsentrasi sukrosa (30, 60, 90 dan 120 g/L). Hasil penelitian menunjukkan bahwa BAP dan sukrosa secara tunggal serta interaksinya berpengaruh nyata terhadap multiplikasi tunas in vitro. Pengaruh konsentrasi BAP 2 mg/L lebih homogen dibandingkan perlakuan BAP 1 dan 3 mg/L. Sukrosa 30 g/L merupakan konsentrasi terbaik untuk multiplikasi tunas. Umbi mikro terbanyak terdapat pada perlakuan BAP 1 mg/L + sukrosa 30 g/L tetapi cenderung mengalami penurunan jika konsentrasi sukrosa dinaikkan pada konsentrasi BAP tetap. Pada perlakuan BAP 2 dan 3 mg/L jumlah umbi mikro yang terbentuk cenderung meningkat seiring dengan peningkatan konsentrasi sukrosa.Kata kunci: satoimo, tunas in vitro, umbi mikro, benzilaminopurin, sukrosa
EMBRIOGENESIS SOMATIK IN VITRO DAN REGENERASI PLANLET DARI TIGA VARIETAS ALFALFA (Medicago sativa L.) Nawfetrias, Winda; Pinardi, Djatmiko; Rosdayanti, Henti; Sulastri, Sulastri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 6, No 1 (2019): June 2019
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.917 KB) | DOI: 10.29122/jbbi.v6i1.3348

Abstract

In Vitro Somatic Embryogenesis and Plantlet Regeneration of Three Varieties of Alfalfa (Medicago sativa L.)ABSTRACTAlfalfa (Medicago sativa L.) is a valuable plant as a source of food for animal, forage, pharmaceutical, medicine, food supplement, and human consumption.  In vitro selection technology combined with induction or spontaneous mutagenesis has been effective in altering or isolating genetic variability for desirable characters.  Consequently, a reproducible in vitro propagation technique of that plant is mandatory. The aim of the research was to obtain information on the embryogenic callus induction, somatic embryogenesis, and plantlet regeneration of three varieties of alfalfa. The results showed that an optimum embryogenic callus induction (82%) was obtained on Murashige & Skoog (MS) basal medium containing 2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin and 2 ppm a-naphthaleneacetic acid (NAA). Those embryogenic calli could subsequently develop into somatic embryos, which germinated and regenerated into normal plantlets on R1 medium consisting of MS nutrients without the addition of plant growth regulator.Keywords: alfalfa, callus, embryogenic, plantlets, regeneration ABSTRAKAlfalfa (Medicago sativa) adalah tanaman berharga sebagai sumber makanan untuk hewan, yaitu hijauan pakan ternak, farmasi, obat-obatan, suplemen makanan dan konsumsi manusia. Teknologi seleksi in vitro yang dikombinasikan dengan induksi atau mutagenesis spontan telah terbukti efektif dalam mengubah atau mengisolasi variabilitas genetik untuk karakter yang diinginkan. Oleh sebab itu, keberhasilan teknik perbanyakan in vitro yang telah terbukti dapat direproduksi dari tanaman tersebut menjadi syarat yang harus terpenuhi. Tujuan dari penelitian ini adalah untuk mendapatkan informasi mengenai induksi kalus embriogenik, embriogenesis somatik dan regenerasi planlet dari tiga varietas alfalfa. Hasil penelitian menunjukkan bahwa induksi kalus embriogenik optimal (82%) didapat pada media Murashige & Skoog (MS) dengan  2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin dan 2 ppm a-naphthaleneacetic acid (NAA). Kalus embriogenik tersebut dapat membentuk embrio somatik, embrionya berkecambah dan beregenerasi membentuk planlet normal pada perlakuan media R1 yaitu nutrisi MS tanpa penambahan zat pengatur tumbuh.Kata Kunci: alfalfa, embriogenik, kalus, planlet, regenerasi
EMBRIOGENESIS SOMATIK IN VITRO DAN REGENERASI PLANLET DARI TIGA VARIETAS ALFALFA (Medicago sativa L.) Sulastri, Sulastri; Nawfetrias, Winda; Pinardi, Djatmiko; Rosdayanti, Henti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.917 KB) | DOI: 10.29122/jbbi.v6i1.3348

Abstract

In Vitro Somatic Embryogenesis and Plantlet Regeneration of Three Varieties of Alfalfa (Medicago sativa L.)ABSTRACTAlfalfa (Medicago sativa L.) is a valuable plant as a source of food for animal, forage, pharmaceutical, medicine, food supplement, and human consumption.  In vitro selection technology combined with induction or spontaneous mutagenesis has been effective in altering or isolating genetic variability for desirable characters.  Consequently, a reproducible in vitro propagation technique of that plant is mandatory. The aim of the research was to obtain information on the embryogenic callus induction, somatic embryogenesis, and plantlet regeneration of three varieties of alfalfa. The results showed that an optimum embryogenic callus induction (82%) was obtained on Murashige & Skoog (MS) basal medium containing 2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin and 2 ppm a-naphthaleneacetic acid (NAA). Those embryogenic calli could subsequently develop into somatic embryos, which germinated and regenerated into normal plantlets on R1 medium consisting of MS nutrients without the addition of plant growth regulator.Keywords: alfalfa, callus, embryogenic, plantlets, regeneration ABSTRAKAlfalfa (Medicago sativa) adalah tanaman berharga sebagai sumber makanan untuk hewan, yaitu hijauan pakan ternak, farmasi, obat-obatan, suplemen makanan dan konsumsi manusia. Teknologi seleksi in vitro yang dikombinasikan dengan induksi atau mutagenesis spontan telah terbukti efektif dalam mengubah atau mengisolasi variabilitas genetik untuk karakter yang diinginkan. Oleh sebab itu, keberhasilan teknik perbanyakan in vitro yang telah terbukti dapat direproduksi dari tanaman tersebut menjadi syarat yang harus terpenuhi. Tujuan dari penelitian ini adalah untuk mendapatkan informasi mengenai induksi kalus embriogenik, embriogenesis somatik dan regenerasi planlet dari tiga varietas alfalfa. Hasil penelitian menunjukkan bahwa induksi kalus embriogenik optimal (82%) didapat pada media Murashige & Skoog (MS) dengan  2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin dan 2 ppm a-naphthaleneacetic acid (NAA). Kalus embriogenik tersebut dapat membentuk embrio somatik, embrionya berkecambah dan beregenerasi membentuk planlet normal pada perlakuan media R1 yaitu nutrisi MS tanpa penambahan zat pengatur tumbuh.Kata Kunci: alfalfa, embriogenik, kalus, planlet, regenerasi
MULTIPLIKASI TUNAS DAN INDUKSI UMBI MIKRO SATOIMO (Colocasia esculenta (L.) Schott) PADA BEBERAPA KONSENTRASI SUKROSA DAN BENZILAMINOPURIN Maretta, Delvi; Handayani, Dwi Pangesti; Rosdayanti, Henti; Tanjung, Armelia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.75 KB) | DOI: 10.29122/jbbi.v3i2.150

Abstract

Taro or Satoimo (Colocasia esculenta (L) Schott var antiquorum) is an alternative of non-rice food. To support saitomo mass cultivation in several regions in Indonesia, shoot multiplication and induction of satoimo microtuber through in vitro technique is amongst the stage to be undertaken. The aims of this study were to determine the effect of BAP (benzylaminopurine) and sucrose for shoot multiplication and microtuber induction of in vitro culture of satoimo. The experiment was arranged in two factors: BAP (0, 1, 2 and 3 mg/L) and sucrose (30, 60, 90 and 120 g/L). The result showed that the single effect of BAP or sucrose and interaction of both significantly increased the number of shoots. The effect of 2 mg/L BAP was more homogeneous than that of 1 and 3 mg/L BAP. Sucrose with the concentration of 30 g/L was the best concentration for shoot multiplication. The highest number of microtuber was achieved with 2 mg/L BAP + 30 g/L sucrose treatments, but tended to decrease due to increasing sucrose concentration. In 2 and 3 mg/L BAP treatments, the number of microtuber increased along with the increasing sucrose concentration.Keywords: satoimo, in vitro shoot, microtuber, benzylaminopurine, sucrose ABSTRAKSatoimo (Colocasia esculenta (L) Schott var antiquorum) merupakan bahan pangan alternatif non-beras. Untuk mendukung produksi massal satoimo di beberapa wilayah di Indonesia, multiplikasi tunas dan induksi umbi mikro secara in vitro merupakan tahapan yang harus dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh BAP dan sukrosa terhadap multiplikasi tunas dan induksi umbi mikro satoimo dalam kultur in vitro. Perlakuan terdiri dari 4 taraf konsentrasi BAP (0, 1, 2 dan 3 mg/L) dan 4 taraf konsentrasi sukrosa (30, 60, 90 dan 120 g/L). Hasil penelitian menunjukkan bahwa BAP dan sukrosa secara tunggal serta interaksinya berpengaruh nyata terhadap multiplikasi tunas in vitro. Pengaruh konsentrasi BAP 2 mg/L lebih homogen dibandingkan perlakuan BAP 1 dan 3 mg/L. Sukrosa 30 g/L merupakan konsentrasi terbaik untuk multiplikasi tunas. Umbi mikro terbanyak terdapat pada perlakuan BAP 1 mg/L + sukrosa 30 g/L tetapi cenderung mengalami penurunan jika konsentrasi sukrosa dinaikkan pada konsentrasi BAP tetap. Pada perlakuan BAP 2 dan 3 mg/L jumlah umbi mikro yang terbentuk cenderung meningkat seiring dengan peningkatan konsentrasi sukrosa.Kata kunci: satoimo, tunas in vitro, umbi mikro, benzilaminopurin, sukrosa
GROWTH AND WOOD TRAITS EVALUATION OF 15-YEAR-OLD TENGKAWANG (Shorea spp.) TREE STANDS IN GUNUNG WALAT UNIVERSITY FOREST, WEST JAVA, INDONESIA Fifi Gus Dwiyanti; Rosdayanti, Henti; Yulita, Kusumadewi Sri; Rachmat, Henti Hendalastuti; Ayyasy, Yahya; Muharam, Karima Fauziah; Rahman, Mohamad Miftah; Adzkia, Ulfa; Siregar, Iskandar Zulkarnaen
Indonesian Journal of Forestry Research Vol. 11 No. 2 (2024): Indonesian Journal of Forestry Research
Publisher : Association of Indonesian Forestry and Environment Researchers and Technicians

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2024.11.2.243-258

Abstract

Gunung Walat University Forest (GWUF) in Sukabumi Regency, Indonesia, plays a crucial role in providing various ecosystem services. Five important Shorea trees, i.e., S. stenoptera, S. mecisopteryx, S. pinanga, S. palembanica, and Shorea leprosula have been planted in GWUF as an effort for its conservation and object of research. An evaluation of the adaptability and suitability of these species to the GWUF ecosystem, as well as their wood characteristics, needs to be carried out regularly. Therefore, the study aimed to examine the growth performances and physical wood properties of five Shorea species, i.e., Shorea stenoptera, S. mecisopteryx, S. pinanga, S. palembanica, and S. leprosula at the age of 15-year-old planted in GWUF. The results indicated that S. leprosula exhibited the best growth performance in terms of average diameter (19.64 cm), volume (0.27 m3), slenderness (126.58), and wood density (0.94 g/cm3), and S. stenoptera showed the best performance in average height (23.35 m). While the poor performance was shown by S. palembanica in terms of average diameter (6.73 cm), height (11.15 m), volume (0.02 m3), wood density (0.87 g/cm3), and specific gravity (0.45), and S. stenoptera in terms of average slenderness (202.73). In addition, significant differences in tree height, diameter, volume, wood density, specific gravity, and moisture content were found in S. palembanica compared with other species. The relationship between the growth and physical wood properties parameters varied between species. The study revealed that planting the five Shorea species in GWUF is suitable for increasing vegetation cover and conserving the species.
Co-Authors Adzkia, Ulfa Armelia Tanjung, Armelia Ayyasy, Yahya Delvi Maretta, Delvi DWI PANGESTI HANDAYANI, DWI PANGESTI Fifi Gus Dwiyanti ISKANDAR ZULKARNAEN SIREGAR KUSUMADEWI SRI YULITA Muharam, Karima Fauziah Pinardi, Djatmiko Pinardi, Djatmiko Rachmat, Henti Hendalastuti Rahman, Mohamad Miftah Sulastri Sulastri Winda Nawfetrias, Winda