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All Journal Public Health of Indonesia International Journal of Applied Biology Medical Laboratory Technology Journal Jurnal Endurance: Kajian Ilmiah Problema Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Health Information : Jurnal Penelitian Borneo Journal of Medical Laboratory Technology Jurnal Teknologi Kesehatan Molucca Medica Mestaka: Jurnal Pengabdian Kepada Masyarakat Jurnal Inovasi, Pemberdayaan dan Pengabdian Masyarakat Media Penelitian dan Pengembangan Kesehatan

Reni Yunus, Reni

Department Of Nursing, Polytechnic Of Health Kendari, Southeast Sulawesi, Indonesia

Author-ID : 606392

Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Uji Bioaktivitas Ekstrak Daun Tawa Ndokulo (Kleinnhovia hospita Linn) Terhadap Bakteri Enteropatogenik Yunus, Reni; Malik, Nurhayu
Jurnal Endurance Vol 4, No 1 (2019): Jurnal Endurance : Kajian Ilmiah Problema Kesehatan
Publisher : Lembaga Layanan Pendidikan Tinggi (LLDIKTI) Wilayah X

Biodiversity in Indonesia stored potential nutritious plants that can be used for the treatment and its potential further development. One plant in southeast Sulawesi, which is an endemic plant is tawa ndokulo (Kleinnhovia hospita L) has been studied. This plant containing chemical compound saponin,cardenolin, bufadienol and anthraquinone. This study aimed to determine the effectiveness of tawa ndakulo leaf extract (Kleinnhovia hospita L) against enteropathogenic bacteria. This was an experimental laboratory, to design a one-shot case study is a design study with the treatment of the variables followed by observation or measurement of the independent variables. Test bioactivity tawa ndokulo leaf extract showed levels Minimal inhibitory tawa ndokulo leaf extracts against E.coli is at a concentration of 35%. While the minimal inhibitory concentration tawa ndokulo leaf extract against Salmonella thypu bacteria are at a concentration of 55%. Based on Test bioactivity, tawa ndokulo leaf extracts showed antibacterial activity against E. coli and Salmonella thypi. Biodiversitas di Indonesia tersimpan potensi tumbuhan berkhasiat yang dapat dimanfaatkan potensinya untuk pengobatan dan pengembangan lebih lanjut. Salah satu tanaman di Sulawesi tenggara yang merupakan tanaman endemik adalah tawa ndokulo ( Kleinnhovia hospita L). Tanaman ini telah diteliti mengandung senyawa kimia saponin, cardenolinn, bufadienol dan antrakinon. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun tawa ndakulo (Kleinnhovia hospita L) terhadap bakteri enteropatogenik.Penelitian ini merupakan eksperimental laboratory, dengan desain one-shot case study yaitu suatu desain penelitian dengan perlakukan terhadap variabel yang diikuti dengan pengamatan atau pengukuran terhadap variabel independen. Uji bioaktivitas ekstrak daun tawa ndokulo menunjukkan Kadar Hambat Minimal ekstrak daun tawa ndokulo terhadap bakteri E.coli adalah pada konsentrasi 35 %. Sedangkan kadar hambat minimal ekstrak daun tawa ndokulo terhadap bakteri Salmonella thypi adalah pada konsentrasi 55 %. Berdasarkan uji bioaktivitas ini, diketahui bahwa bahwa ekstrak daun tawa ndokulo efektif sebagai antibakteri terhadap bakteri E.coli maupun bakteri Salmonella thypi.

Cemaran Bakteri Gram Negatif pada Jajanan Siomay di Kota Kendari Reni Yunus; Ruth Mongan; Rosnani Rosnani
Medical Laboratory Technology Journal Vol. 3 No. 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Foodborne disease caused by food infection is one of health concern which cause of consumption of contaminated foods that containing living microorganisms. There are several kinds of microorganisms that can cause food infection, one of them is Salmonella sp, a negative Gram basil shaped-bacteria as the cause typhoid and paratyphoid diseases. The purpose of this study is isolate and identify negative Gram bacteria on snacks in the traditional market Anduonuhu Kendari. This research is a descriptive study with dumplings samples which sale on the traditional Market Anduonohu as population. Samples were taken as a total sampling with pour plate method was used for bacterial isolation. All 10 samples were shown positive result which growth in Brain Heart Infusion Broth media as 7 of them are positively grow in Salmonella Shigella Agar with negative Gram basil-shaped bacteria was found under the microscope after Gram staining, suspected as negative Gram baceria. It can be concluded that 7 (70%) out of of 10 dumplings samples were contaminated negative Gram bacteria. This research can be continued by identifying other pathogenic bacteria which are the bacteria that cause food borne disease.

Inhibition of Juice Komba-Komba (Chromolaenaodorata) Leaf Against Growth of Bacteria Staphylococcus aureus Reni Yunus; Muhammad Hilam; Satya Darmayani
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.6062

Nowadays many plants can be used as traditional medicines to overcome various diseases including infections, because the use of traditional medicines is relatively safer compared to drugs derived from chemicals. One of the local plants in Southeast Sulawesi that has been empirically used as a medicine for wounds is a race of plants. Komba-komba leaves (Chromolaena odorata) containcompounds alkaloid, flavonoids, tannins, phenols, triterpenoids, saponins and steroids which have their respective functions in inhibiting the growth of microorganisms or antibacterial. This study aims to determine the inhibition of komba-komba leaf extract (Chromolaena odorata) on the growth ofbacteria Staphylococcus aureus. This type of research is experimental laboratories. The method used was agar diffusion with 5 treatment concentrations, namely komba-sheep leaf extract concentration (Chromolaena odorata) 20%, 40%, 60%, 80% and 100%, positive control (tetracycline) and negative control (aquadest) and repetition . The results showed that there was a inhibition zone of komba-komba leaf extract (Chromolaena odorata) on the growth ofbacteria Staphylococcus aureus at a concentration of 20% at 2 mm, a concentration of 40% at 3.7 mm, a concentration of 60% at 4.7 mm, a concentration of 80% 6.2 mm and a concentration of 100% by 8 mm.

Efektivitas Sari Buah Belimbing Wuluh (Averrhoa Bilimbi Linn) sebagai Larvasida Alami terhadap Nyamuk Aedes sp. Reni Yunus; Afrindayanti Afrindayanti; Petrus Petrus
Health Information : Jurnal Penelitian Vol 10 No 2 (2018): Juli-Desember
Publisher : Poltekkes Kemenkes Kendari

Bilimbi (Averrhoa Bilimbi Linn) is a plant that commonly used as a daily cooking spice. A. Bilimbi has the potential as a larvicide, as it containing alkaloids, saponins, and flavonoids. This study aimed to determine the effectiveness of A. Bilimbi juice as a larvicide for Aedes sp. This research was an experimental laboratory study with a post test only control group design, the objects were divided into two groups, the treatment group and the control group. Total samples was 350 larvae of Aedes sp.. The results showed that the effectiveness of the A. Bilimbi juice on the death of Aedes sp. larvae within 24 hours was ineffective at concentrations of 2%, 3%, and 4% while effective at concentrations of 5%, 6%, 7%, and 8%. The calculation of probit model showed that the value of LC50 is 4.080% and LC90 is 7.014%. A. Bilimbi juice is effective as a natural larvicide for the Aedes sp.

Analisis Perbedaan Jumlah Nyamuk Aedes sp. yang Terperangkap pada Ovitrap Standar dan Ovitrap Bambu Askrening; Reni Yunus; Susilawati
Health Information : Jurnal Penelitian Vol 12 No 1 (2020): Januari-Juni
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36990/hijp.vi.149

Dengue Hemorrhagic Fever (DHF) is an infectious disease that occurs because of dengue virus infection, which is carried by the vector Aedes sp. Kendari City is one of the dengue-endemic areas in Southeast Sulawesi Province. Some control programs of Aedes sp. so far less successful because it places more emphasis on fogging for adult mosquitoes. There are methods of controlling Aedes sp. without insecticide which has so far reduced vector density in some countries, namely the use of egg traps (ovitrap). This study aims to determine differences in the number of eggs trapped in standard ovitrap and bamboo ovitrap. This research is an observational study with a cross-sectional method. The results showed that the p-value 0.6168 which meant that there was no difference between mosquito eggs trapped in standard ovitrap and bamboo ovitrap.

Analisis Bakteri Coliform Pada Air Minum Isi Ulang Di Wilayah Poasia Kota Kendari Askrening Askrening; Reni Yunus
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 13 No. 2 (2017): September
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The existence of safe drinking water now begin to be limited in number, so that the existence of refill drinking water depotbecomes an alternative water treatment process, in principle, should be able to eliminate all kinds of pollutants, includingcoliform bacteria, which is a group of bacteria used as an indicator of pollution, waste and conditions which are not good forwater. This study aims to determine coliform bacteria contamination in refill drinking water depot in Poasia Kendari. The typeof research is descriptive analytic approach using MPN (Most Probable Number). These samples included 10 samples takenwith saturated total sampling method. Data were analyzed by using frequency distribution. The results showed that thesamples were positive in getting as much as 6 samples (60%) and over the limit contamination and negative samples ingetting as many as four samples (40%), so it can be concluded that from coliform bacteria identification of 10 samples of refilldrinking water in Poasia Kendari, 6 samples were identified contaminated with coliform bacteria and microbial contaminationwas over the limit of PERMENKES 492 / Menkes / Per / IV.2010.

Gambaran Angka Kematian Larva Nyamuk Aedes aegypti dengan Pemberian Kulit Jeruk Purut (Citrus hystrix) Sebagai Larvasida Alami Tuty Yuniarty; Reni Yunus
Jurnal Teknologi Kesehatan (Journal of Health Technology) Vol. 12 No. 2 (2016): September
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.109 KB)

Dengue Fever (DF) and Dengue Haemorrhagic Fever (DHF) are infectious diseases caused by Dengue Virus and transmitted by the bite of Aedes Aegypti. One way to prevent the spreading of the DHF is by controlling its vectors. The best and most effective way to control the vector is with the eradication of Aedes aegypti larvae, which is called larviciding using temephos (abate powder). However, the application of temephos continually will pollute the environment and increase the resistance of larvae to pesticide so that natural pesticides are needed. One of them is by using Kaffir lime peel (Cytrushystric) which contains limonoids. Limonoid is known as toxic to the larvae of Aedes aegypti. The objective of this research is to find out the mortality rate of larvae with the concentrations of Kaffir lime peel as a natural pesticide. The method of this research is descriptive research, by using 200 Aedes aegypti larvae. Those larvae were divided into 3 concentrations of 3%, 5% and 7% of Kaffir lime peel with twice repetition and 1 % of temephos as a control. Each concentration contained 25 larvae in 100 ml of water. The observations were conducted by counting the dead larvae. The results showed that the average of the mortality rate of larvae at a concentration of 3% was 9 larvae (36%), a concentration of 5% were 21 of larvae (84%) and 7% were 24 of larvae (96%). It can be concluded that the mortality rate of Aedes Aegypti larvae by using Kaffir lime as natural larvacide at the concentration of 7% was the highest out of 3 concentrations with mortality of larvaewere 24 of 25 larvae (96%).

Analisis Kualitatif Morfologi Eritrosit Pada Apusan Darah Edta (Ethylene Diamine Tetraacetic Acid) Untuk Pemeriksaan Segera (0 Jam) Dan Pemeriksaan Ditunda (2 Jam): Qualitative Analysis of Erythrocyte Morphology in EDTA (Ethylene Diamine Tetraacetic Acid) Blood Smears For immediate examination (0 hours) and delayed examination (2 hours) Reni Yunus; Fina Astina; Fonnie E. Hasan
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 1 (2022): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pemeriksaan preparat apus darah tepi merupakan bagian yang penting dari rangkaian pemeriksaan hematologi. Keunggulan dari pemeriksaan apus darah tepi ialah mampu menilai berbagai unsur sel darah tepi seperti morfologi sel (eritrosit, leukosit, dan trombosit.Tujuan penelitian ini adalah mengetahui analisis morfologi eritrosit pada apusan darah EDTA pada penyimpanan 0 jam (segera) dan penyimpanan 2 jam. Jenis penelitian yang digunakan adalah penelitian Quasi eksperimental design. Penelitian ini tidak memberikan variasi perlakuan ataupun pengukuran berulang sebelum dan sesudah perlakuan, melainkan hanya memberikan variasi waktu penundaan pengecatan pada sampel. Populasi dalam penelitian ini adalah Mahasiswa Jurusan Teknologi Laboratorium Medis Tingkat III Poltekkes Kemenkes Kendari yaitu sebanyak 48 orang, sedangkan sampel dalam penelitian sebanyak 12 responden. Terdapatperbedaanjumlahsampel yang normal dan tidak pada pengamatan morfologi eritrosit untuk pengamatan berdasarkan ukuran dan pewarnaan, baik pada penyimpanan 0 jam maupun 2 jam.

RELATIONSHIP BETWEEN KNOWLEDGE, ACTION OF FAMILY IN DCB (DRAIN, CLOSE, AND BURY) PROGRAM, AND THE EXISTENCE OF EGGS OF AEDES AEGYPTI MOSQUITO ON OVITRAP IN KANDAI KENDARI, INDONESIA Yunus, Reni; Rosanty, Anita
Public Health of Indonesia Vol. 2 No. 4 (2016): October - December
Publisher : YCAB Publisher & IAKMI SULTRA

Background: Dengue Hemorrhagic Fever (DHF) is a contagious disease caused by the dengue virus infection through Aedes aegypti mosquito. DHF often causes epidemics and Extraordinary Events (EE) in tropical and subtropical regions, including Indonesia.Objective: This study aims to examine the relationship between knowledge and society action on the prevention of dengue with the amount of mosquitoes trapped in ovitrap in Kandai, Kendari, Indonesia.Methods: The research method was an observational study with cross sectional design. The research was conducted at Kandai village, Kendari on May to July 2014. It was 87 homes with 87 families were recruited as sample size for this study. Ovitrap was used inside and outside of their homes.Results: DCB family knowledge and the existence of Aedes sp mosquitoes eggs on ovitrap were analyzed and observed in this study. Chi square test showed that X2 count value (1.261) was less than X2 table value (3.814) at error level 5% (Î± = 0.05) indicated that Ha was rejected and Ho was accepted. Meanwhile, Chi square test for the action of the family about DCB and the existence of eggs Aedes sp mosquito on ovitrap showed that X2 count value (4.115) was greater than X2 table value (3.814) at the error level 5% (Î± = 0.05), indicated that Ho was rejected and Ha was accepted.Conclusions:"¨ It was concluded that (1) there was no relationship between DCB family knowledge and the existence of Aedes sp mosquitoes eggs on ovitrap, and (2) there was a relationship between the action of family about DCB and the existence of aedes sp mosquitoes eggs on ovitrap in Kandai Kendari.

HEMOGLOBIN LEVELS IN PREGNANT WOMEN LIVING IN COASTAL AND PLATEAU AREAS, KENDARI, INDONESIA Rosanty, Anita; Yunus, Reni
Public Health of Indonesia Vol. 3 No. 3 (2017): July - September
Publisher : YCAB Publisher & IAKMI SULTRA

Background: Insufficient hemoglobin levels are one of the most vulnerable health problems during pregnancy. Hemoglobin levels less than 11 g/dL indicate anemia in pregnant women. However, it is assumed that there is a difference of hemoglobin levels between pregnant women in coastal and plateau areas. Objective: To compare the hemoglobin levels in pregnant women who live in the coastal and plateau areas in the working area of the Community Health Center of Mata, Kendari. Methods: This was an observational analytic research with cross sectional approach, conducted in May-June 2014. There were 38 pregnant women using quota sampling, divided into 19 respondents in coastal areas and 19 respondents in plateau areas. Blood sampling was performed in each respondent, and categorized into mild, moderate, and severe anemia. Data were analyzed using chi-square test. Results: The proportion of pregnant women with anemia in the working area of the Community Health Center of Mata was 94.74%. Chi-square test showed p-value 0.307 (>0.05). Conclusion: There was no significant difference in hemoglobin levels in pregnant women in coastal and plateau areas in the working area of the Community Health Center of Kendari district.

Co-Authors Afrindayanti Afrindayanti Angriani Fusvita Anita Rosanty, Anita Askrening Askrening Askrening Darmayani, Satya Fauzi, Ahmad Zil Fina Astina Fonnie E. Hasan Fristiohady, Adryan Gobel, Sri Yunanci Van Hasan, Aswiro Hasan, Fonnie E Hasan, Fonnie Esther Hasnah, Nur Ikbal Ikbal Jumasran, Jumasran Kartini Kartini Magdalena Magdalena Muh. Ikhsan, Muh. Muhammad Hilam Munanto, Risman Nurhayani, St. NURHAYU MALIK, NURHAYU Nurtimasiah, Wa Ode Petrus Nugroho DS Puspita, Sarlita Rahayu, Dian Yuniar Syanti Ruth Mongan Ruth Mongan, Ruth Sari, Julianti Isma Satya Darmayani Supiati Supiati Supryatno, Adi Susilawati Trees, Trees Tuty Yuniarty Tuty yuniarty, Tuty Wijayati, Fitri

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