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All Journal HAYATI Journal of Biosciences Jurnal Sains dan Seni ITS BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Indonesian Journal of Chemistry Akta Kimia Indonesia Journal of Fibers and Polymer Composites Sewagati
Adi Setyo Purnomo
Departemen Kimia, Institut Teknologi Sepuluh Nopember
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Public Health Social Sciences Veterinary Other

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Biodegradation of 1,1,1-Trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) by Mixed Cultures of White-Rot Fungus Ganoderma lingzhi and Bacterium Pseudomonas aeruginosa Erly Grizca Boelan; Adi Setyo Purnomo
HAYATI Journal of Biosciences Vol. 26 No. 2 (2019): April 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.683 KB) | DOI: 10.4308/hjb.26.2.90

Abstract

This study investigated the biodegradation of 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) by mixed cultures white-rot fungus Ganoderma lingzhi BMC 9057 and bacteria Pseudomonas aeruginosa. Cultures bacteria P. aeruginosa with various volumes 1, 3, 5, 7, and 10 ml (1 ml ≈ 1.53 x 109 bacteria cells/ml cultures) was added into 10 ml G. lingzhi cultures for degrading DDT. After 7 d incubation, DDT was degraded about 100% with addition of 5, 7, and 10 ml of P. aeruginosa culture into G. lingzhi. Two metabolites; 1,1-dichloro-2,2-bis (4-chlorophenyl) ethane (DDD) and 1-chloro,2-2-bis (4-chlorophenyl) ethylene (DDMU) were detected from mixed cultures G. lingzhi and P. aeruginosa as metabolite products of DDT. This research indicated that mixed cultures of whiterot fungus G. lingzhi and P. aeruginosa could be used to degrade DDT.
Phytochemical, Antibacterial, and Antioxidant Activities of Anthurium Hookerii leaves Extracts Atmira Sariwati; Inayah Fitri; Adi Setyo Purnomo; Sri Fatmawati
HAYATI Journal of Biosciences Vol. 26 No. 3 (2019): July 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.777 KB) | DOI: 10.4308/hjb.26.3.101

Abstract

Many plants of the family of Araceae possess significant benefit as medicinal plants. Anthurium hookerii is herbaceous genus of the family of Araceae. A. hookerii leaves were extracted with five dissimilarity solvents (methanolic, water, ethyl acetate, n-hexane, and dichloromethane). The extracts were evaluated for their phytochemical, total phenolic contents, and antibacterial potential. The presences of tannins and saponins were found in all crude extracts. The steroid was only found in dichloromethane extract, whereas flavonoid was obtained in methanol and water extracts. Besides; methanol, ethyl acetate, water, and n-hexane extracts showed triterpenoid contents. Alkaloid presences in ethyl acetate, methanolic, dichloromethane, and water extracts. The total phenol content was examined by Follin-Ciocalteu assay, which varied from 9.52-76.56 mg/g GAE. The highest total phenolic was found in methanol extract. Antioxidant activity was calculated based on diphenyl picryl hydrazyl radical scavenging ability that showed the scavenging activity with range 7.24-66.11%, which the methanoilic extract have the excellent antioxidant potential (IC50 232.90 µg/ml). Antibacterial activity of leaves extracts of A. hookerii was screened based on disc diffusion method. Water extract showed the wide spectrum antibacterial potential. Klebsiella sp., Bacillus subtilis, Pripioni agnes, and Strepticoccus mutans with maximum diameter of inhibition zone 10.30, 14.20, 9.60, and 15.10 mm, respectively.
Antimicrobial Activity of Sonneratia ovata Backer Laili Khumaidah; Adi Setyo Purnomo; Sri Fatmawati
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.467 KB) | DOI: 10.4308/hjb.26.4.152

Abstract

Sonneratia, a genus of mangroves from family Lythraceae, is reported for number of high biological activity including antimicrobial. Sonneratia ovata Backer is one of the species which traditionally used by Indonesian people for the treatment of some diseases. In this research, the investigation about biological activity of S. ovata Backer as anti-microbial has been performed. The methanol extract of S. ovata Backer is highly potential as antimicrobial against gram positive bacteria, S. aureus, with IC50 value of 7.7 μg/ml which is higher than of Ampicillin as positive control with IC50 value of 37.8 μg/ml. Furthermore, stigmasterol one of the major compound of S. ovata Backer has been isolated from the methanol extract of the stem bark of S. ovata Backer. Therefore, S. ovata Backer is proven to have high activity as antimicrobial, and highly potential to be a new source of antimicrobial agent.
Antibacterial and Toxicity Activities of Indonesian Herbal Medicine Extracts Used for Postpartum Treatment Wiwit Denny Fitriana; Sri Banun Titi Istiqomah; Devi Anggraini Putri; Taslim Ersam; Adi Setyo Purnomo Purnomo; Nurlatifah Nurlatifah; Sri Fatmawati
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.232

Abstract

Postpartum treatments have been used by local women in Indonesia for some time now. One commonly used postpartum treatment is the consumption of a traditional herbal medicine called PHM-1 during the first 40 days after childbirth. In addition, a second medicine known as PHM-2 is taken for days 41-80. However, sufficient scientific evidence about the benefits of this postpartum herbal medicine does not exist. In this study, the antibacterial abilities of PHM-1 and PHM-2 therefore was evaluated and showed positive potential for both. Low IC50 values were obtained against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Rhodopseudomonas palustris, Ralstonia pickettii, and Staphylococcus epidermidis and compared with chloramphenicol as a positive control. The antibacterial activity of both PHM-1 and PHM-2 against R. pickettii had the highest inhibitory activity as evidenced by the lowest IC50 values of 11.16 and 6.26 μg/ml, respectively. Furthermore, an in vivo acute toxicity test showed that PHM-1 produced a low LD50 value of 3.28 g/kg BW for both of male and female mice. These results support the use of both PHM-1 and PHM-2 as antibacterial treatments for postpartum women.
Biodegradation of DDT by Co-cultures of Pleurotus eryngii and Pseudomonas aeruginosa Diana Maulianawati; Adi Setyo Purnomo; Ichiro Kamei
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.240

Abstract

Dichloro-diphenyl-trichloroethane (DDT) is a synthetic insecticide that widely used around the world, which has a negative effect on human health and the environment. The objective of this research was to investigate the ability of bacterium Pseudomonas aeruginosa in co-culturing with white-rot fungus Pleurotus eryngii to degrade DDT. The various volume of P. aeruginosa (1 ml ≈ 1.5 x 109 CFU) were added into 10 ml of P. eryngii culture for a 7-days of incubation. Approximately 82% of degradation of DDT were obtained from co-cultures with the adjunct of 10 ml of P. aeruginosa during the 7-day incubation period, which had the best ratio of optimization of 0.57. The confrontational assay showed that P. aeruginosa gave no effect on the growth of P. eryngii (0.39 cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by co-cultures bacterium P. aeruginosa and fungus P. eryngii. This study indicated that bacterium P. aeruginosa can be used to enhance DDT degradation by whire-rot fungus P. eryngii.
Role of Fe2+-dependent Reaction in Biodecolorization of Methyl Orange by Brown-rot Fungus Fomitopsis pinicola Adi Setyo Purnomo; Asranudin; Nela Rachmawati; Hamdan Dwi Rizqi; Refdinal Nawfa; Surya Rosa Putra
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.146-154

Abstract

The involvement of Fenton reaction on biodegradation of methyl orange (MO) by brown-rot fungus Fomitopsis pinicola was investigated based on Fe2+-dependent reaction. The degradation of MO (final concentration 75 mg/L) was performed in mineral salt media with and without Fe2+ with incubation period at 0, 7, 14, 21, and 28 days. Degradation analysis was performed using UV-Vis Spectrophotometer and LC-TOF/MS. F. pinicola decolorized MO in a medium containing Fe2+ and a medium that lacked the mineral, at percentages of 89.47% and 80.08%, respectively. The optimum decolorization occurred after 28 days of incubation with the fungus on the presence of Fe2+, indicated that the presence of Fe2+ enhanced MO degradation with assumed to correlate with Fenton reaction. Two metabolites were detected through the LC-TOF/MS analysis, namely 4-(2-(4-(dimethyliminio)-2-hydroxycyclohexa-2,5-dien-1-ylidene) hydrazinyl) phenolate (m/z 258, RT: 1.28 min, compound 1) and 4-(2-(4-(dimethyliminio) cyclohexa-2,5-dien-1-ylidene) hydrazinyl) benzenesulfonate (m/z 391, RT: 2.70 min, compound 2). Compound 1 was a transformation product of hydroxylation and methylation, compound 2 was a product of dehydroxylation and desulfonation. This study indicated that the transformation of the metabolite structures was involved hydroxyl radical (OH.) and enzymatic mechanisms, which involved Fe2+-dependent reaction.
Pengaruh Tongkol Jagung sebagai Media Pertumbuhan Alternatif Jamur Tiram Putih (Pleurotus ostreatus) terhadap Aktivitas Antimikroba A'in Qurrota A'yunin; Refdinal Nawfa; Adi Setyo Purnomo
Jurnal Sains dan Seni ITS Vol 5, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.616 KB) | DOI: 10.12962/j23373520.v5i1.15964

Abstract

Jamur tiram putih mampu tumbuh pada substrat yang mengandung lignoselulosa. Pengurangan jumlah kayu sengon sebagai media pertumbuhan jamur tiram putih terjadi akibat semakin meningkatnya permintaan masyarakat terhadap jamur tiram putih, sehingga diperlukan media pertumbuhan alternatif yang memiliki kandungan lignoselulosa. Tongkol jagung dipilih sebagai media pertumbuhan alternatif jamur tiram putih karena mengandung lignoselulosa. Penelitian ini bertujuan untuk mengetahui pengaruh tongkol jagung sebagai media pertumbuhan alternatif jamur tiram putih terhadap aktivitas antimikroba pada bakteri Bacillus subtilis dan Pseudomonas aeruginosa. Variasi perbandingan tongkol jagung dan kayu sengon yang digunakan sebagai media tanam pertumbuhan jamur dalam penelitian ini adalah 25:75, 50:50, 75:25, dan 100:0 (b/b). Jamur tiram putih diekstrak menggunakan pelarut metanol. Ekstrak metanol jamur tiram putih dianalisa aktivitas antimikroba menggunakan metode dilusi broth. Hasil menunjukkan bahwa variasi komposisi tongkol jagung mempengaruhi aktivitas antimikroba pada ekstrak metanol jamur tiram. Jamur tiram putih pada semua variasi komposisi media tanam tidak memiliki aktivitas antimikroba terhadap bakteri P. aeruginosa. Jamur tiram putih dengan variasi komposisi media tanam 50% tongkol jagung memberikan hasil aktivitas antimikroba tertinggi terhadap bakteri B. subtilis dengan persentase penghambatan sebesar 11,02%.
Pengaruh Sabut Kelapa Sebagai Media Pertumbuhan Alternatif Jamur Tiram Putih (Pleurotus ostreatus) Terhadap Aktivitas Antimikroba Siti Mamluatus Sa'adah; Refdinal Nawfa; Adi Setyo Purnomo
Jurnal Sains dan Seni ITS Vol 5, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.295 KB) | DOI: 10.12962/j23373520.v5i1.15963

Abstract

Pengaruh variasi komposisi sabut kelapa sebagai media pertumbuhan jamur tiram putih (Pleurotusostreatus) terhadap aktivitas antimikroba telah diteliti. Varias ikomposisi serbuk kayu sengon dan sabut kelapa yang digunakan adalah 0:100 (SK-1), 25:75 (SK-2), 50:50 (SK-3), 75:25 (SK-4) dan 100:0 (SK-5). Jamur tiram putih diliofilisasi dan diekstrak dengan metode maserasi menggunakan pelarut metanol. Ekstrak metano lP. Ostreatus diujiaktivitas antimikroba menggunakan metode dilusi cair. Hasil  yang diperoleh menunjukkan bahwa konsentrasi antara variasi komposisi sabut kelapa sebagai media pertumbuhan jamur tiram putih mempengaruhi aktivitas antimikroba. Hasil uji antimikroba menggunakan metode dilusi cair menunjukkan % penghambatan tertinggi pada SK-3 sebesar 72,380 % terhadap B. subtilis, sedangkan P. aeruginosas ebesar 133,696%. Komposisi variasi SK-3 menunjukkan aktivitas antimikroba terbaik terhadap bakteri B. subtilis dan P. aeruginosa
Pengaruh Campuran Ampas Tebu dan Alang-Alang (Imperata cylindrica) sebagai Media Pertumbuhan terhadap Kandungan Nutrisi Jamur Tiram Putih (Pleurotus ostreatus) Ishmatun Naila; Adi Setyo Purnomo
Jurnal Sains dan Seni ITS Vol 5, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (544.509 KB) | DOI: 10.12962/j23373520.v5i2.16175

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh ampas tebu dan alang-alang (Imperata cylindrica) sebagai media pertumbuhan jamur tiram putih (Pleurotus ostreatus) terhadap kandungan nutrisinya. Ampas tebu dan alang-alang dipilih sebagai media pertumbuhan alternatif, karena tidak hanya mengandung lignoseluosa, tapi juga tersedia berlimpah di lingkungan. Variasi komposisi ampas tebu:alang-alang yang digunakan adalah 75:25 (A1); 50:50 (A2); 25:75 (A3); 0:100 (A4); dan 100:0 (A5). Pada penelitian ini, kandungan nutrisi yang dianalisis adalah kadar air, abu, protein kasar, lemak kasar, dan total karbohidrat. Metode yang digunakan adalah termo-gravimetri, furnis, Kjeldahl, dan ekstraksi Soxhlet. Hasil yang didapatkan yaitu kadar air dan lemak kasar terendah sebesar 77,29% dan 0,09% pada variasi A4, sedangkan kadar abu dan protein kasar tertinggi didapatkan sebesar 1,16% dan 11,70% pada variasi A3 dan A2. Sementara kadar karbohidrat terendah didapatkan pada variasi A5 sebesar 2,23%. Variasi media A4, dengan komposisi 100% alang-alang, lebih disukai untuk menghasilkan jamur tiram dengan kandungan nutrisi yang paling baik.
Pengaruh Penambahan Bakteri Bacillus subtilis terhadap Biodegradasi DDT oleh jamur Pelapuk Putih Pleurotus eryngii Dwi Lestari; Adi Setyo Purnomo
Jurnal Sains dan Seni ITS Vol 5, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.681 KB) | DOI: 10.12962/j23373520.v5i2.16177

Abstract

Pengaruh penambahan bakteri Bacillus subtilis terhadap biodegradasi DDT oleh jamur pelapuk putih Pleurotus eryngii telah diteliti. B. subtilis ditambahkan ke dalam 10 mL kultur P. eryngii masing-masing dengan jumlah 1, 3, 5, 7, dan 10 mL (1 mL ≈ 1,573 x 108 sel). Recovery DDT dilakukan dengan menggunakan metode ekstraksi cair-cair. Analisa jumlah degradasi dan metabolit produk yang terbentuk menggunakan instrumen HPLC dan GC-MS. Degradasi DDT tertinggi ditunjukkan pada penambahan 3 mL B. subtilis dengan jumlah degradasi sebesar 88,26%, sedangkan degradasi DDT terendah terjadi pada penambahan 1 mL B. subtilis dengan jumlah degradasi sebesar 41,84%. Metabolit produk yang dihasilkan dari proses degradasi ini adalah DDD [l,l dikloro- 2,2-bis(p-klorofenil) etana] dan DDE [l,l-dikloro-2,2- bis(p-klorofenil) etilena].
Co-Authors A'in Qurrota A'yunin Abdillah, Salsabilah Putri Ade Restuani Rahma Adisya Salsabila Aldianita, Ananda Krisna Alvin Rahmad Widiyanto Alvin Romadhoni Putra Hidayat Alya Awinatul Rohmah Alya Awinatul Rohmah Ariati, Nafla Rahma Dian Asranudin Asranudin Asranudin Asranudin Asranudin Asranudin Asranudin Asranudin Atmira Sariwati Aulia Ulfi Ayundra, Rezania Azzahira, Sabira Badzlin Nabila Badzlin Nabilah Ceisar Andrian Putra Ciptoaji, Yusril Rizqi Citra ‘Aaqilatul Fa’aidah Dalia Allouss Diah Trisna Rahayu Lestari Didik Prasetyoko Dini Ermavitalini Djohana Tri Lois Moyo Dwi Lestari Egar Pamela Eka Pratiwi Yuniarti Erly Grizca Boelan Fadhil Adinata Fadlan, Arif Fahimah Martak Fahimah Martak Fajriah Fajriah Fanda Luvita Sari Faradita Eka Puspitasari Farid Kamal Muzaki Fazad, M. Haikhal Ferdy Muhammad Gading, Anjani Puspa Gunawan, Triyanda Halimah, Adedila Hamdan Dwi Rizqi Hamzah Fansuri Hamzah Fansuri Hendro Nurhadi Herdayanto Sulistyo Putro Herdayanto Sulistyo Putro Hery Purnobasuki Hibatullah, Faizah Holilah Holilah Ichiro Kamei Iman, Muhammad Ilham Nur Inayah Fitri Indana Madaniyah Tsani Indrapaksi, Tengku Ikhwan Alfajri Irma Citra Mayangsari Iwan Adi Indrawan Karina Harley Aulia Rizky Khulaida, Khoridatul Laili Khumaidah Laudy Nasya Yanuar Lavida Aisya Salvanora Lea Kristina Anggraeni Lisman Suryanegara Maulianawati, Diana Mawardani, Alfiyyana Nurrahma Muchammad Tamyiz Mufidah, Iftin Farekana Muhammad Lukman Hakim Muhammad Lukman Hakim, Muhammad Lukman Muhammad Rasyid Abdullah Nabilah Farah Azzahra Naila, Ishmatun Nawfal, Mohammad Fadl Johara Nela Rachmawati Nourma Sulistyowati Novia Nurul Hidayah Nur Khoiriyah Nurhayati, Alfia Nurlatifah Nurlatifah Nurul - Widiastuti Nurul Widiastuti Octaviani, Wulan Nur Oktafia, Galuh Krisna Pangayom, Danang Luhur Prasetiyowati, Mega Pratama, Silvia Abdi Pratiwi, Tasya Resa Putra, Ananda Satria Eka Putra, Muhammad Farhan Lucky Putri, Aulia Malihah Putri, Devi Anggraini Putro, Herdayanto Sulistyo Refdinal Nawfa Refdinal Nawfa Rizqi, Hamdan Dwi Robiatuz Zuniar Rohmah, Alya Awinatul Sabilla, Wardatus Salsabila, Finna Ananda Sigmatresna Wasatya Silvia Abdi Pratama Siti Mamluatus Sa'adah Sotya Rawiningtyas Sri Banun Titi Istiqomah Sri Fatmawati Sri Fatmawati SRI FATMAWATI Sri Fatmawati Sri Fatmawati Surya Rosa Putra Surya Rosa Putra, Surya Rosa Suryo Wiyono Syafri Izzat Abidiy Taslim Ersam Tatas, Tatas Taufiq Rinda Alkas Triono Bagus Saputro, Triono Bagus Weni Sri Ekowati Widhayanti, Nur Ari Widiya Nur Safitri Wilujeng, Susi Agustina Wiwit Denny Fitriana Yediya, Hanna Yulianengtias, Andira Yulinar Dwi Nur Azizah Yuliya Andriani Nanggewa Yunita Alfiyati Firdausa Yusuf Syahril Alam Zahra Salsabila Zahra, Ema Azzah Aulia Zakiyyah Yasmin Salsabila