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All Journal METAMORFOSA Journal of Biological Sciences Jurnal MIPA Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioedukatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bioedukasi: Jurnal Pendidikan Biologi Jurnal Sains dan Edukasi Sains Proceeding Biology Education Conference Bioed : Jurnal Pendidikan Biologi BIOCHEPHY: Journal of Science Education Al-Khidmah Jurnal Pengabdian Masyarakat AGRIC Engagement: Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pengabdian Kepada Masyarakat

Sri Kasmiyati

Universitas Kristen Satya Wacana

Author-ID : 516996

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Perkecambahan Biji Dan Pertumbuhan Kecambah Varietas Sorgum (Sorghum bicolor L.) Pada Cekaman Krom Heksavalen Kasmiyati, Sri; S, Santosa; Priyambada, Irfan Dwidja; Dewi, Kumala; Sandradewi, Rintawati
Bioma : Berkala Ilmiah Biologi Vol. 17, No.1, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

In contrast to other toxic trace metals, Cr has received little attention. Since valence level of chromium determines its toxicity, chromium is categorized as unique heavy metal. Chromium hexavalent (Cr6+) has the biggest toxicity among other valence levels. Seed germination and seedling growth are sensitive to heavy metal stresses. This research aimed to find out the responses of seed germination and seedling growth of 12 sorghum varieties toward Cr6+. Seed germination and seedling growth experiment was done on 12 sorghum varieties (Badik, UPCA-1, Keris, Keris M3, hegari Genjah, Gambela, Selayer, Sangkur, Mandau, Batari, Kawali, dan Numbu), planted in petridishes with Cr6+ treatment in form of chromate (K2CrO4) and dichromate (K2Cr2O7) compounds with 0, 50 and 500 mg of Cr/l concentration for a week. The parameters measured were the number of seeds germinate each day; the length of radicle and plumule, and fresh and dry weight at the end of experiment. The results showed that higher concentration of Cr6+ both in form of dichromate and chromate, significantly decreasedÂ the length of radicle and plumule, fresh and dry weight, and SVI (seedling vigor index) value. However, index germination (GI) value and percentage of germination of the 12 varieties sorgum significantly increased in the treatment of 50 mg Cr/l Cr6+ in form of dichromate and chromate. The treatment of dichromate compound showed bigger effect than chromate toward variables of seed germination and seedling growth of sorghum. It was noticed that 12 sorghum varieties possessed an integrated complex of adaptation to cope with the range of form of compound and concentration of Cr6+. Based on the responses of seed germination and seedling growth, Kawali, Hegari, Keris, Keris M3, Mandau, and Selayer varieties was more susceptible toward Cr6+ toxicity, and Sangkur, Selayer, Batari, and Numbu was more tolerant than other varieties. Keywords : chromate, dichromate, Sorghum bicolor, seedling, hexavalent chromium

PERTUMBUHAN ARTEMISIA VULGARIS SECARA KULTUR PUCUK PADA MEDIUM DENGAN KANDUNGAN MIOINOSITOL DAN EKSTRAK KHAMIR Kasmiyati, Sri; Herawati, Maria M.; Kristiani, Elizabeth B.E.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

The effects of mioinositol and yeast extract were studied to assess their influence on growth of plantlets of Artemisia vulgaris by shoot culture. The plants regeneration of A. vulgaris were established by removing the nodes of stem and growing in MS multiplication medium with 1 ppm kinetin and 1 ppm NAA for 4 weeks. Shoots were induced for roots on MS treatment medium supplemented with mioinositol and yeast extract, added with 2 ppm IBA. Combination of four levels mioinositol concentration (mg/l): 100, 200, 300, and 400, and four levels of yeast extract concentration (mg/l): 0, 200, 300, and 400 were simultaneously added. Plantlets (2 weeks) were sub cultured on semi liquid MS medium. Plantlets were harvested on 6 weeks old. Measured parameters were fresh weight of plantlets. Data were analyzed using ANOVA followed by HSD test (p=95%). The results showed that the treatment of mioinositol and yeast extract were not significantly influenced on fresh weight of plantlets. Yeast extract was not influenced the growth of plantlets. The growth and morphogenesis of plantlets A. vulgaris were induced in treatment 100 ppm mioinositol, and addition mioinositol were higher than 100 ppm not significantly influenced the growth of plantlets.

RESPON ENZIM ANTIOKSIDATIF SONCHUS OLERACEUS TERHADAP CEKAMAN KROM PADA MEDIA TANAM BERBEDA Sucahyo, Sucahyo; Kasmiyati, Sri
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3661

ABSTRACTSonchus oleraceus is a weed that is capable of living in chromium contaminated environments, so their physiological, biochemical and molecular responses can be used as biomarkers of chromium stress. Changes in the activity of antioxidative enzymes can be used as a biomarker of biochemical against heavy metal stress.The objective of this research was to know the response of antioxidative enzymes activity in S. oleraceus subjected to Cr stress on different planting media. The research was done in 2 factors i.e. planting media types and Cr compound types (Cr3+and Cr6+). Parameters measured were antioxidative enzymes activity i.e catalase (CAT), ascorbate peroxidase (APX), superoxidase dismutase (SOD), and glutathion reductase (GR), dissolved protein content in leaves, and content of Cr6 + and Cr total in roots and shoots.The data were analyzed with a two way ANOVA using SAS 9.1 32-bit for windows. The results indicated that activity of antioxidative enzymes (CAT, APX, SOD and GR) of S. oleraceus leaves tend to increase under Cr stress condition. The Cr6+ treatment of 10 mg/L significantly increased the antioxidative enzyme activity of CAT, APX, SOD and GR in S. oleraceus leaves. The antioxidative enzyme activity (APX, SOD and GR) S. oleraceus grown on soil medium containing sludge was higher than that grown on soil media and on the mixture of sludge: soil (1: 1). Under Cr stress conditions, the roots and shoots of S. oleraceus grown on sterile sand media, soil and the mixture of sludge:soil (1:1) accumulate Cr6+ and Cr total. The accumulation of Cr6+ was found the highest in shoots, meanwhile accumulation of Cr total was found the highest in the roots. The Cr treatments and planting media also significantly influenced the soluble protein content in S. oleraceus leaves.Key words: antioxidant, oxidative stress, chromium, weed, soluble protein

Characterization of Ficus benjamina and Artocarpus heterophyllus Proteases as Potential Rennet Alternatives Sukmana, Andreas Binar Aji; Widyaningrum, Indy; Lani, Rischa Karmila; Kasmiyati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23516

Rennet, a milk coagulant exhibiting proteolytic activity, is a crucial component in cheese industries. Its price and availability have discouraged the growth of some small scale cheese industries. Therefore, an alternative for rennet will be beneficial for the industries. Among other sources, plant proteases offer some advantages as rennet alternatives. This study aimed to investigate the potential of plant proteases obtained from the latex as potential rennet alternatives. A total of six plants from the genusÂ FicusÂ andÂ ArtocarpusÂ were screened for their proteolytic activity and milk coagulating ability. The screening indicated that all six tested plants displayed proteolytic activity at various levels, but onlyÂ Ficus benjaminaÂ andÂ Artocarpus heterophyllusÂ produced a firm milk curd. Hence, bothÂ F. benjaminaÂ andÂ A. heterophyllusÂ were determined to be the most potential. Further characterizations suggested thatÂ F. benjaminaÂ andÂ A. heterophyllusÂ protease were optimum at pH 7.0 also at 50Â°C and 40Â°C, respectively. At their optimum conditions,Â both proteasesÂ exhibited a lower MCA/PA ratio than that of the rennet. This study contributed to scientific knowledge development by becoming the first to characterize the optimum conditions of F. benjamina and A. heterophyllusâ€™ proteases, investigate their MCA/PA ratio, and compare their activity against commercial rennet. The examination of their potentials as rennet alternatives could benefit small cheese industries and the communities.

The Antioxidant Content and Activity of Various Plant Organs of Kitolod (Isotoma longiflora) Egarani, Grahita Rezhi; Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23888

Antioxidants play a crucial role in human health owing to their ability to neutralize oxidative reactions. Kitolod (Isotomalongiflora) is one of the medicinal plants that can be used as a source of antioxidant. The aim of study wasto compare the content of antioxidant compounds (flavonoids, phenolics, chlorophylls, carotenoids, and ascorbic acid) in various organs of the kitolod plant. The plant organs studied includingroots, stems, leaves, flowers, and fruits. The extraction of each plant organ wasconducted by maceration technique using ethanol as a solvent at the room temperature for 48 hours. The analysis of total flavonoids, phenolics, and ascorbic acid was performed by colorimetry method using AlCl3, Folin, and sulfosalicyclic acid reagent respectively, while chlorophylls and carotenoids was determined by direct colorimetry method with methanol as a solvent. The antioxidant activity of various plant organs of kitolod was analyzed using the DPPH method. The highest total flavonoid, phenolic, chlorophyll, and carotenoid compounds werefound in leaves, respectively by 10.48, 1.46, 7.25, and 56.98 ppm. The highest ascorbic acid content and antioxidant activity were obtained fromfruits.The research findings provide new and important information aboutthe contents and antioxidant activity of the secondary metabolites (flavonoid, phenolic, chlorophyll, carotenoid, and ascorbic acid) in each organ of kitolod plant. The information from the results of this study can be used to increase the medicinal value ofkitolod plants.

Analisis Hubungan Kekerabatan Fenetik serta Potensi Kegunaan Varietas Portulaca oleracea dan Portulaca grandiflora di Desa Grogol, Kelurahan Dukuh, Kota Salatiga Prasgi, Henokh Christian; Pratama, Dimas Seno Bagus; Kapitarauw, Agnesya Giovani Putri Cendana; Kasmiyati, Sri
Jurnal MIPA Vol 11, No 1 (2022)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.v11i1.35054

Portulacaceae merupakan tumbuhan dikotil dari famili Portulacaceae yang dapat hidup di berbagai kondisi tanah sehingga disebut gulma serta memiliki bentuk dan variasi warna bunga yang beragam untuk dijadikan tanaman hias. Portulaca memiliki berbagai kegunaan untuk kesehatan, sehingga diperlukan suatu program pemuliaan tanaman yang didasarkan pada kekekerabatan menggunakan karakter morfologinya. Tujuan penelitian ini untuk mengetahui hubungan kekerabatan atau fenetik serta sifat kegunaan dari beberapa varietas Portulaca oleracea dan Portulaca grandiflora di Desa Grogol, Kelurahan Dukuh, Kota Salatiga dengan metode Analysis of Cluster dan Euclidean Matrix of Index Dissimilarity. Penelitian ini dilaksanakan di Desa Grogol, Kelurahan Dukuh, Kecamatan Sidomukti, Kota Salatiga, Jawa Tengah. Jenis penelitian deskiptif eksploratif. Karakter yang digunakan sebanyak 22 buah yang dianalis denggan program PAST. Hasil penelitian menunjukkan 6 anggota famili Portulacaceae yang dianalisis dengan metode Analysis of Cluster membentuk 2 klaster utama yaitu klaster 1 Portulaca grandiflora (Oranye, Putih, dan Pink) serta klaster 2 Portulaca oleracea (Kuning. Merah, dan Pink) dengan indeks kesamaan terbesar pada P. grandiflora Oranye dengan P. grandiflora Putih serta P. grandiflora Putih dengan P. grandiflora Pink (99%), sedangkan yang terendah antara Portulaca grandiflora Oranye dengan Portulaca oleracea Merah (94,8038%). Pigmen, faktor genetik, dan lingkungan menyebabkan perbedaan karakter. Kedua spesies ini berpeluang besar dalam pengembangan obat-obatan karena senyawa dan sifatnya.

Pengaruh Herbisida Metil Metsulfuron Terhadap Struktur Komunitas Alga Perifiton Satria Dharmawan Kustia Dewa; Sucahyo Sucahyo; Sri Kasmiyati
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p01

Agricultural activity can’t be separated from synthetic herbicide. Some synthetic herbicide use metsulfuron methyl as active ingredient that works to change chemical materials in weed’s tissues so able to exterminate the tissue or disordering physiological system that needed for survivability of weeds. Because of that effect a question arises whether this compound also can affect non target organisms like periphyton algae. Periphyton is a group of microscopic organisms that grows attached to surface of objects or water plants, not penetrate the substrate, staying or moving on the substrate surfaces. One of main compilers of periphyton community is algae. Periphyton algae in water has significant role to determining primary productivity and as bioindicator of water quality. Some research that has been done shows if some active ingredient in particular concentration significantly affects toward primary productivity of periphyton algae and able to inhibit growth of certain algae species. This research was conducted to find out effects of metsulfuron methyl herbicide on periphyton algae community structure. Parameters measured are concentration of chlorophyll a, density, richness, diversity, evenness, and domination indices also periphyton algae species identification. Research conducted in laboratory scale use samples taken from Rawa Pening Lakes, Semarang District, Central Java. Observation time is 21 days with herbicide exposure at 0, 0.05, 0.1, 0.2, and 0.4 mg/L. Results show that periphyton algae community structure more impacted by exposure period of herbicide actve ingredient metsulphuron methyl than concentration that used in research. Keywords: Periphyton Algae, Metsulfuron Methyl, Community Structure

Uji Aktivitas Antibakteri Campuran Ekstrak Biji Kelor (Moringa oleifera) dan Daun Kersen (Muntingia calabura) terhadap Pseudomonas aeruginosa dan Bacillus subtilis Syuhuud Arumbinang Wajdi; Sri Kasmiyati; Susanti Puji Hastuti
Journal of Tropical Biodiversity and Biotechnology Vol 2, No 1 (2017): June
Publisher : Universitas Gadjah Mada

Moringa oleifera and Muntingia calabura leaves have been reported to have an antibacterial activity that could inhibit the growth of gram positive and negative bacteria. However, the antibacterial activity of mixed extracts of M. oleifera seeds and M. calabura leaves has not been widely reported. The purpose of this study was to test antibacterial activity of the mixed extract of M. oleifera seeds and M. calabura leaves on the growth of Pseudomonas aeruginosa and Bacillus subtilis. The experiment was conducted by agar disc diffusion method using three groups of extract treatments i.e. M.oleifera seeds extract, M.calabura leaves extract, and mixed extracts of M. oleifera seeds and M. calabura leaves with a ratio of 1: 1 (v / v). The extraction of M. oleifera seeds and M. calabura leaves was conducted by soxhlation method and using ethanol as solvent. The three groups of extract treatments with a concentration of 400 ppm, 800 ppm, 1200 ppm, and 1600 ppm were tested on P. aeruginosa. The antibacterial activity test of M. oleifera seed extract against B. subtilis carried out at the level of concentrations i.e. 150 ppm, 300 ppm, 450 ppm, 600 ppm, and 750 ppm, meanwhile, M. calabura leaves extract was done at concentration 1500 ppm, 3000 ppm, 4500 ppm, 6000 ppm, and 7500 ppm. The result showed that the three groups of extract treatments possess antibacterial activity against P. aeruginosa. The mixed extracts of M. oleifera seeds and M. calabura leaves with a ratio of 1: 1 (v / v) at level concentration of 400 ppm and 800 ppm were tested against P. aeruginosa significantly increased, and at concentrations of 1200 ppm and 1600 ppm significantly decreased the inhibition diameter of bacterial growth than the other extracts treatments. The antibacterial test results of M.oleifera seeds extract and M.calabura leaves extract against B. subtilis shows that increased concentrations of the extract significantly increase the inhibition diameter of bacterial growth especially at high concentrations ( 600 ppm and 750 ppm) on M. oleifera seeds extract, as well as 6000 ppm and 7500 ppm in M. calabura leaves extract.

Dekolorisasi pewarna tosca menggunakan koagulan ferro sulfat dan lumpur aktif dari pabrik teksil di salatiga pada kondisi aerob Agustien Sri Noerwahju; Vincentia Irene Meitiniarti; Sri Kasmiyati
Jurnal Ilmu Lingkungan Vol 17, No 3 (2019): November 2019
Publisher : School of Postgraduate Studies, Diponegoro Univer

Industri tekstil mengeluarkan air limbah dengan kualitas COD, padatan tersuspensi, warna yang relatif buruk dan mengandung logam berat yang bergantung pada zat warna yang digunakan. Saat ini Perda Prop Jawa Tengah no 5 tahun 2012, belum mengatur parameter warnasebagai parameter uji namun limbahberwarna harus tetap diolah karena menyangkut masalah estetika. Salah satu pewarna yang sulit didegradasikan melalui pengolahan biologi maupun kimia/fisika adalah pewarna Turquise Blue atau Tosca. Penelitian ini bertujuan untuk mempelajari pengurangan absorbansi warna, kandungan COD dan TSS pada limbah tekstil sintetik yang mengandung pewarna tosca dengan penambahan ferro-sulfat dan lumpur aktif pada waktu tinggal tertentu dengan proses aerob. Penelitian ini menggunakan limbah cair sintetik yang mengandung pewarna tosca dengan konsentrasi warna yang sama dengan warna air limbah aslinya (absorbansi warna diukur pada l 536 nm). Pengolahan dilakukan dengan mengkombinasikan proses Ferro-sulfat dan lumpur aktif pada kondisi aerob dengan sistem Sequencing bacth reactor (curah berurutan). Pengambilan sampel dilakukan pada jam ke 48, dan jam ke 72. Parameter yang diamati adalah absorbansi warna, COD dan TSS. Efisiensi pengurangan parameter tersebut dibandingkan pada semua perlakuan. Dari hasil penelitian didapat efisiensi penurunan terbaik untuk COD, TSS, dan absorbansi warna, berturut-turut adalah sebesar 79, 60, dan 52% pada pengolahan jam ke 72.

Aplikasi Mikoriza Glomus fasciculatum dan Glomus mosae dengan Tumbuhan Sorghum bicolor dalam Penyerapan Cr VI Vini Kurnia Ramadhani; Sri Kasmiyati; Susanti Pudji Hastuti
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Chrommium is a non-essential metal which is highly toxic to microorganisms and plants. One of the efforts to improve the productivity of the soil is done by inoculating the microorganisms in the soil, that is with using mycorrhizal fungi. Sorghum bicolor is a plant that quite often found in Indonesia and resistant to heavy metals, for the example is chromium. Research of the mycorrhizal association of Glomus fasciculatum or Glomus mosae with plants in reducing heavy metal contamination has been done, but the mechanism of resistance to heavy metals Cr VI in Sorghum bicolor which associated with mycorrhizal fungi Glomus mosae and Glomus fasiculatum has not been explained yet. The study was conducted experimentally using a randomized block design with three treatment concentrations of Cr VI (0 ppm, 5 ppm, 10 ppm) and 3 treatment \of mycorrhiza (without mikiroza; Glomus mosae; Glomus fasiculatum). Each treatment was made 5 replications. Plants kept in polybags arranged randomly. The study was conducted for one month, and the data were analyzed with ANOVA analysis with SPSS applications. The study aims to compare the effect of giving mycorrhizal Glomus Glomus fasiculatum and mosae to plant Sorghum bicolor on media contaminated with heavy metals chromium in various concentrations. The results showed that G.mosae and G.fasciculatum can increase plant tolerance to heavy metals. The presence of mycorrhizae cause the concentration of Cr VI is lower than in the absence of mycorrhiza on S.bicolor. There are significant differences between treatments without giving mycorrhiza and giving mycorrhiza (P ≤ 0.05). G. fasciculatum symbiotic with S.bicolor be able to reduce Cr VI more than G.mosae although the leaves has not significant difference.

Co-Authors Agna Sulis Krave Agustien Sri Noerwahju Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Anna Helena Sabandar Araujo, Otilda Fatima Ariyanto, Prasetyo Eko Betty Elok, Elizabeth Cahyaningrum, Desti Christian Cantika Glodia Debora Kristiani Rahardjo Desti Christian Cahyaningrum Dewi Prabawaningrum Djohan Djohan Djurubasa, Dani Karel Marthin Egarani, Grahita Rezhi Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Elizabeth Bety E.K Fanny Nurwahidah, Cantika Fatima Araujo, Otilda Gabriella L. Mataradja Hapsari Sekar Widiantari, Olive Ie, Johanes Juan Yutama Putra Indrianasari, Putri Irfan Dwidja Priyambada, Irfan Dwidja Jokosulistyo Wartanto Juliana Sarioa Kapitarauw, Agnesya Giovani Putri Cendana Keliat, Natalia Rosa Kumala Dewi Lani, Rischa Karmila Margareta Prameswati Maria Marina Herawati Monica Mangudis, Vicharisti Nabila, Nabila Novellasari, Firdiana Penina Intansari Prasgi, Henokh Christian Pratama, Dimas Seno Bagus Priyayi, Desi Fajar Purwoko, Agus Putra Ie, Johanes Juan Yutama Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rintawati Sandradewi, Rintawati Risya Pramana Situmorang Rully Adi Nugroho Santosa S, Santosa Satria Dharmawan Kustia Dewa Seleiman, Mahmoud F. Setya Magdalena, Ajeng Siahaya, Esty Vicariat Sucahyo Sucahyo Sucahyo Susanti Pudji Hastuti Susanti Puji Hastuti Syuhuud Arumbinang Wajdi Tambunan, Roslinda Kristin Trisianna, Jessica Ayu Trisianna Vincentia Irene Meitiniarti Vini Kurnia Ramadhani Wahyu Hari Kristiyanto Widyaningrum, Indy Yohanes Martono Yolanda Halim, Vivian

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