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All Journal Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) JURNAL FARMASI DAN KESEHATAN INDONESIA
Suwidjiyo Pramono
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KROMATOGRAFI CAIR KINERJA TINGGI Nining Sugihartini; Achmad Fudholi; Suwidjiyo Pramono; Sismindari Sismindari
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.743 KB) | DOI: 10.12928/pharmaciana.v4i2.1567

Abstract

High Performance Liquid Chromatography (HPLC) was one of analytical methodscommonly used to determine the concentration of epigallocatechin gallate (EGCG) on green teaextract. The method must be validated in order to fit to its purpose. The aim of this research was toprove that the used method has selectifity, liniearity, precise, accurate and know limit of detection(LOD) and limit of quantification (LOQ) is acceptable. The selectivity of analytical method wasdetermined by calculating the resolution value between two peak. Data from 10 μg/mL and100 μg/mL with 5 replicates would give precition and accuration. Precition was known from CV value and accuration was known from recovery value in each concentration. Liniearity was knownfrom regression linear between concentration and wide area of peak. From regresion linear couldcalculate LOD and LOQ. Research show that method of analyse have selectificity withRs= 2.27>1.5; liniearity with r= 0.99; precision with CV 8.74% at concentration 200 µg/mL and3.74% at concentration 500 µg/mL; accuration with recovery 99.76% at concentration 200 µg/mLand 100.52% at concentration 500 µg/mL and the value of LOD is 33.28 μg/mL and LOQ is110.93 μg/mL.
FORMULASI GRANUL KOMBINASI EKSTRAK TERPURIFIKASI HERBA PEGAGAN (Centella asiatica) (L.) Urban) dan HERBA SAMBILOTO (Andrographis paniculata) (Burm.f.) Nees) Lina Widiyastuti; Suwidjiyo Pramono; Agung Endro Nugroho
Media Farmasi: Jurnal Ilmu Farmasi Vol 11, No 2: September 2014
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (569.786 KB) | DOI: 10.12928/mf.v11i2.1874

Abstract

Herba pegagan (Centella asiatica (L.) Urban dan herba sambiloto (Andrographis paniculata (Burm.f.) Ness) merupakan tanaman yang potensial untuk dikembangkan menjadi bahan obat. Diperlukan suatu formulasi yang baik agar kedua herba dapat dengan mudah digunakan dengan tetap memperhatikan standart parameter kualitasnya. Penelitian ini bertujuan untuk mendapatkan formula granul kombinasi ekstrak terpurifikasi herba pegagan dan sambiloto menggunakan Avicel PH 101 (pengisi-penghancur) dan PVP K-30 (bahan pengikat) dengan menggunakan factorial design 22. Granul dibuat dengan perbandingan ekstrak terpurifikasi 30:70. Hasil penelitian menunjukkan bahwa kombinasi Avicel PH 101 dan PVP K-30 serta interaksinya dapat menghasilkan granul dengan kecepatan alir 11,50 ± 0,41 g/detik, daya serap 26,58 ± 1,21 mg/menit, kandungan lembab  4,6% ± 0,21, indeks pengetapan 16,33% ± 0,58. Diperoleh formula optimum dengan bobot kombinasi ekstrak terpurifikasiherba pegagan dan herba sambiloto 360 mg, Avicel PH 101 360 mg dan PVP K-30 18 mg menghasilkan granul dengan sifat fisik yang memenuhi persyaratan. 
ISOPROTERENOL SEBAGAI MODEL INFARK MIOKARDIAL AKUT PADA TIKUS WISTAR Wahyu Widiyaningsih; Sugiyanto Sugiyanto; Sitarina Widyarini; Suwidjiyo Pramono
Jurnal Ilmu Farmasi dan Farmasi Klinik Prosiding Seminar Nasional "Peluang Herbal Sebagai Alternative Medicine"
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.658 KB) | DOI: 10.31942/jiffk.v0i0.1339

Abstract

ABSTRACT Decreased oxygen supply and necrosis in the heart muscle cells can lead to acute myocardial infarction. Free radical compounds can lead to oxidative stress in the heart muscle and lead to myocardial infarction. Isoproterenol is a non-selective β-adrenergic agonist can lead to acute myocardial infarction in large doses. The purpose of this study was to validate the model of acute myocardial infarction. This study using male Wistar rats aged two months as the test animal. Male Wistar rats divided into two groups: control group and treatment group. The control group treated with physiological saline and the treatment group treated with isoproterenol 85 mg/kg BW. Isoproterenol administered subcutaneously for two doses with an interval of 24 hours. After 48 hours of the isoproterenol first administration, rat heart rhythm's records with an electrocardiogram (ECG). Furthermore, the rats sacrificed and performed a necropsy. The left ventricle part in the cross section for the macroscopic observation (extensive infarction) with triphenyl tetrazolium chloride (TTC) staining, microscopic observation of infarction with hematoxylin-eosin staining (HE) and immunohistochemical (IHC) to observe the expression of caspase-3. The ECG of isoproterenol group showed elevation in the ST segment. TTC staining shows the expansion of the infarct area. The HE staining showed the typical image of myocardial infarction and the IHC staining results showed an increase in the expression of caspase-3. The results showed that isoproterenol can be use as a model myocardial infarction with parameters of infarction are ECG changes, TTC staining of infarct broad macroscopic image, a histopathological image of heart and apoptosis with the expression of caspase-3. Key words: Isoproterenol, acute myocardial infarction, cardiac histopathology
UJI EFEK HIPOGLIKEMIK GETAH DAN GEL DAUN LIDAH BUAYA [ALOE VERA (L.) BURM. F.] PADA TIKUS YANG TERINDUKSI STREPTOZOTOSIN Mega Karina Putri; Suwidjiyo Pramono; Agung Endro Nugroho
JURNAL FARMASI DAN KESEHATAN INDONESIA Vol. 1 No. 1 (2021): Jurnal Farmasi dan Kesehatan Indonesia
Publisher : Universitas Kristen Immanuel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61179/jfki.v1i1.143

Abstract

Hiperglikemik merupakan salah satu ciri diabetes mellitus (DM). Penanganan DM agarkadar glukosa darah dapat terkontrol, dilakukan dengan memperhatikan life style dankonsumsi obat, baik sintetik maupun berasal dari tanaman. Salah satu tanaman yang dapatdigunakan adalah lidah buaya [Aloe vera (L.) Burm. f.]. Namun, di dalam lidah buayaterkandung antrakinon, yang mempunyai efek samping berupa laksatif. Untukmenghindari efek tersebut, dilakukan perlakuan dengan memisahkan gel dan getah daunlidah buaya, kemudian dilakukan optimasi dan kombinasi dosis gel dan getah daun lidahbuaya. Kedua ekstrak tersebut kemudian diuji efek farmakologinya dengan parameterkadar glukosa darah. Uji farmakologi dilakukan selama 21 hari pada 40 ekor tikus jantangalur wistar. Sampling darah dilakukan ketika tikus berumur 7, 8, 9 dan 10 minggu.Sampling darah dilakukan dengan pengambilan preprandial dan postprandial. Data kadarglukosa darah yang diperoleh dianalisis statistik dengan uji One way ANOVA. Uji kadarglukosa menunjukkan bahwa gel daun lidah buaya memberikan % potensi penurunankadar glukosa darah preprandial tertinggi yaitu 33,32±1,21%. Dosis getah yangdiberikan, baik tunggal maupun kombinasi dengan gel tidak ditemukan adanya efeksamping laksatif.
Co-Authors Achmad Fudholi Agung Endro Nugroho Lina Widiyastuti Mega Karina Putri Nining Sugihartini Sismindari Sismindari Sitarina Widyarini Sugiyanto Sugiyanto Wahyu Widiyaningsih