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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Jurnal Produksi Tanaman Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Folium : Jurnal Ilmu Pertanian Berkala Penelitian Hayati AGRONISMA
Novi Arfarita
Universitas Islam Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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ISOLATION AND CHARACTERISTIC OF NITROGEN-FIXING BACTERIA AND PHOSPHATE-SOLUBILIZING BACTERIA FROM SOIL HIGH IN MERCURY IN TAILINGS AND COMPOST AREAS OF ARTISANAL GOLD MINE Nuraini, Yulia; Arfarita, Novi; Siswanto, Bambang
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The research was conducted at Brawijaya University and West Nusa Tenggara, from March 2013 to October 2013. The tailings areas of the gold mine contains high mercury (Hg) as much as 1,090 ppm, and living microbes (resistance) exist in a small number in such a condition. Microbial P solvents encountered came from the genus Bacillus with a population of 23 x 103 cfu g-1 and N-fixing bacteria encountered are of the genus Bacillus, with a population of 4 x 103 cfu g-1. Identification of species using Becton Dickinson Phoenix test, both species belong to Bacillus megaterium and Bacillus pumilus. While the waste corn and peanuts that has been composted for 4 weeks acquired P-solubilizing bacteria, such as Enterobacter, Bacillus and Pseudomonas, and N-fixing bacteria found was Pseudomonas and Azotobacter. While testing the activity and antagonism of N-fixing and P-solubilizing bacteria, the result on agar media did not show antagonism in its growth. Bacillus pumilus and Bacillus megaterium effective at 5 % molasses medium with the number of 0.15 x 1012 on seven days of incubation. Keywords: N-fixing bacteria, P-solubilizing bacteria, mercury, phytoremediation
THE APPLICATION OF Trichoderma viride STRAIN FRP 3 FOR BIODEGRADATION OF GLYPHOSATE HERBICIDE IN CONTAMINATED LAND Arfarita, Novi; Djuhari, Djuhari; Prasetya, Budi; Imai, Tsuyoshi
AGRIVITA, Journal of Agricultural Science Vol 38, No 3 (2016): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i3.550

Abstract

In this current study, we observed Trichoderma viride strain FRP3 capability for biodegradation of glyphosate on contaminated land in Indonesia. There were two blank plots that have been involved as representatives of indigenous fungal, that prepared as control (non-contaminated soil) and P1 (GP-contaminated soil) while the treatments were represented by two plots.  Plot 2 (P2) was introduced with conidia suspension of Trichoderma viride strain FRP3 one time application, and plot 3 (P3) was introduced with conidia suspension of Trichoderma viride FRP3 two time applications. At the end of observation, the CFU of two times application was the highest with CFU of 15.97 x 106 gr-1 soil. The CFU of P3 was corresponding to 45% higher than P2 (8.83 x 106 gr-1 soil). The CFU of GP-contaminated soil without conidia suspension application had 0.66 x 106 gr-1soils, only 0.7% and 0.4% corresponding to P2 and P3, respectively. Direct indicator of glyphosate degradation was determined using GC analysis. Within 7 days after Trichoderma viride FRP3 was introduced, glyphosate content of treated soil decreased. This fungal strain provided 48% (P2) and 70% (P3) of glyphosate degradation higher than indigenous soil microbial community (P1) within 28 days of application.
OBSERVASI EFEK EKSTRAK ETANOL DAUN BAMBU JAWA (Gigantochloa atter (Hassk.) Kurz) DENGAN PARAMETER FISIK DAN FISIOLOGI HEWAN UJI TIKUS (Rattus sp.)YANG DIINDUKSI BORAKS Airlangga, Hardadi; Safitri, Endand; Arfarita, Novi
El-Hayah : Jurnal Biologi Vol 5, No 2 (2015): EL-HAYAH (VOL 5, NO 2, Maret 2015)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i2.3023

Abstract

Bamboo Jawa leaves (Gigantochloa atter) is known to contain antioxidant compound sandhas been used as abeverage from generation to generation invarious Asiancountries. While in Indonesia, currently borax widely abusedas a food preservative and food additives. Borax produce exogenous free radicals that can lead to organ damage due to oxidative stress.This current study was to observe the effect of ethanolic extract of G. atter leaf using physiology and physical parameter onthe levels of SOD, MDA and the hepatosite necrosis totalof rats induced by borax. Borax will generate free radicals which cause cell damage through lipid peroxidation there by increasing the levels of MDA and lower SOD antioxidant. Borax metabolism occurs mostlyin theliver, therefore, one of the organs damaged by borax is liver The method of this research was experimental control group posttest only using wistar rats which divided into five groups. The negative control group was induced by aquades. The positive control group was induced by borax 1gr/kg BW and treatment group was induced by borax. Ethanolic extract of G. atter leaf was given with doses of 10mg/ml, 20mg/ml and 40mg/ml for 21 days. After 21 days of treatment, hepatocite necrosis was then observed and counted using microscope trinocular 1000x. SOD examination was conducted by the NBT test while the MDA using TBA test. Data analysis was using one way ANOVA test. G. atter ethanolic extract dosageof 10 mg/ml, 20 mg/ml and 40 mg/mlcould decrease the number of liver cells necrosis. They were 23%, 39% and 63% respectivelly when compared to positive control. Extract ethanolik of G.Atter leaf also could improve SOD and decrease MDA rats serum which induced by borax. The effective dose is 20mg/ml.
ISOLASI DAN IDENTIFIKASI BAKTERI PENGHASIL PROTEASE YANG DISKRINING DARI TERASI ARFARITA, NOVI
El-Hayah : Jurnal Biologi Vol 5, No 3 (2015): EL-HAYAH (VOL 5, NO 3,September 2015)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i3.3098

Abstract

Terasi is a traditional fermented shrimp paste of Indonesia popularly used as an important ingredient in many Indonesian cuisines, and further screened for their protease-producing bacteria. A total of 117 bacterial isolates were retrieved  from 4 terasi samples. When inoculatedusing skim milk agar, 71 isolates (~61%) produced distinct clear zones implying their proteolytic activity. A bacterial isolate  namely Bacillus S2-3 wasselected for further study due to its widest clear zones when thebacterial cells and culture supernatant were used. This strain could grow up to 60oC with a broad pH range of 5 – 9. The optimal growth condition was at pH 6 and 37oC. The enzyme activity was also characterized using azocasein method. The optimal pH of bacterialenzymes was between 6 and 7 whereas the optimal temperature of BacillusS2-3 proteases was 22 – 24oC. For thermostability, the S2-3 enzymes remained active up to 2h at 40 and 50oC. The results ofbiochemical tests, BacillusS2-3 was identified as Bacillussubtilis, later explored it is potential for the purposes for  food industry
Exploration of indigenous free nitrogen-fixing bacteria from rhizosphere of Vigna radiata for agricultural land treatment Novi Arfarita; Anton Muhibuddin; Tsuyoshi Imai
Journal of Degraded and Mining Lands Management Vol 6, No 2 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (793.22 KB) | DOI: 10.15243/jdmlm.2019.062.1617

Abstract

Nitrogen is the most abundant element in the atmosphere, however, most often deficient in agricultural lands. This research was an exploratory to get indigenous non-symbiotic nitrogen-fixing bacteria. Soil samples were collectedfrom rhizosphere of green beans. This study was aimed to determine the bacterial population of the three regions; screening, isolation and selection of free nitrogen-fixing bacteria. Antagonism and pathogenicity tests were performed to observe its potential for a biofertilizer product. The highest number of free nitrogen-fixing bacteria was found from forest soil sample of 2.5x 1011CFU/ml. Screening and isolation process has obtained 10 free nitrogen-fixing isolates. Then was selected into 4 isolates namely SNF4, SNF5, SNF7 and SNF8 according to the ammonia production test qualitatively. When an antagonism activity performed, there was no inhibition zone each other. The pathogenicity test did not show the pathogenic symptom. This study also showed that bacterial isolates obtained significantly affected the germination growth of green beans compared to controls. Possibility, bacteria of this type produced growth hormone for a plant. Strain SNF8 has shown the highest ammonium production then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SNF8 had 99% similarity with Bacillus cereus.
Effects of seaweed waste on the viability of three bacterial isolates in biological fertilizer liquid formulations to enhance soil aggregation and fertility Novi Arfarita; Takaya Higuchi; Cahyo Prayogo
Journal of Degraded and Mining Lands Management Vol 6, No 4 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2019.064.1889

Abstract

Biofertilizer production in Indonesia should fulfil the minimum requirement for being produced and released to the market. Problems occurred when those products are being absent on informing those expiration dates and the viability of microbial activity which then closely related to the quality of the product. Seaweed composted material are potential resources for producing Biofertilizer, but lacking on the optimization on their process as this material contain a various important component for soil and environment. The production of Biofertilizer from seaweed waste required an optimum condition, i.e.: pH and typical microbe which could germinate under specific formulation and temperature. This study aimed to determine the optimum pH in liquid fertilizer formulations made from seaweed waste in the form of composted material, to test the viability of three bacterial isolates and those pathogenicity properties, to examine the effect of metabolites release from bacterial isolates to green bean seed germination. The experimental design used was a completely randomized design with four treatments, which were as follow: P0 as a control (Peptone), RP1 (seaweed waste), RP2 (seaweed waste and glycerol), and RP3 (seaweed waste and PEG). The three bacterial isolates used were: (1) Bacillus licheniformis, (2) Psudomonas plecoglossicida and (3) Pantoea ananatis. This liquid fertilizer biological formulation was stored for 8 weeks at pH 5.5 and temperature 25oC. The results showed that the treatment of RP1 (seaweed waste) had high bacterial viability and could stimulate growth for green bean sprouts. The carrier material for seaweed waste with the addition of glycerol and PEG showed no effect of the disease and symptoms of a pathogenic bacterial consortium on germination of green beans.
Enhancing bare land soil quality using electric induction apparatus in combination with rabbit urine liquid fertilizer application to support garlic (Allium sativum) production Novi Arfarita; Mohammad Jasa Afroni; S Sugiarto; Tsuyoshi Imai
Journal of Degraded and Mining Lands Management Vol 7, No 4 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2020.074.2381

Abstract

The new approach in this study was using electric induction technique to enhance soil fertility of bare land in a combination of rabbit urine application in the form of liquid fertilizer. This approach had been exaggerating soil microbial activities and modifying the exchange capacity of anions and cations in soil. This study aimed to find out the best combination of different duration of electricity induction and rabbit urine liquid fertilizer at different levels of their application. Randomized factorial block design with two factors was used for this study. The first factor was the duration of the electric induction at the rates of 0, 30, 45, and 60 minutes, while the application of rabbit urine as liquid organic fertilizer was at the rates of 0, 100, 150, and 200 mL/L. Plant height, number of leaves and stem diameter, fresh and dry weight of garlic bulb were measured within 3 months. The observations were carried out using destructive and non-destructively approached with intervals of 7 days. The best treatment that gave the highest plant height, number of leaves, stem diameter, tuber diameter, fresh and dry garlic bulb and garlic production was found under the combination of 60 minute of electricity induction and 200 mL/L of urine rabbit application (P<0.05). Also, this treatment was successfully to give garlic production of 8.56 t/ha.
ISOLATION AND CHARACTERISTIC OF NITROGEN-FIXING BACTERIA AND PHOSPHATE-SOLUBILIZING BACTERIA FROM SOIL HIGH IN MERCURY IN TAILINGS AND COMPOST AREAS OF ARTISANAL GOLD MINE Yulia Nuraini; Novi Arfarita; Bambang Siswanto
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i1.422

Abstract

The research was conducted at Brawijaya University and West Nusa Tenggara, from March 2013 to October 2013. The tailings areas of the gold mine contains high mercury (Hg) as much as 1,090 ppm, and living microbes (resistance) exist in a small number in such a condition. Microbial P solvents encountered came from the genus Bacillus with a population of 23 x 103 cfu g-1 and N-fixing bacteria encountered are of the genus Bacillus, with a population of 4 x 103 cfu g-1. Identification of species using Becton Dickinson Phoenix test, both species belong to Bacillus megaterium and Bacillus pumilus. While the waste corn and peanuts that has been composted for 4 weeks acquired P-solubilizing bacteria, such as Enterobacter, Bacillus and Pseudomonas, and N-fixing bacteria found was Pseudomonas and Azotobacter. While testing the activity and antagonism of N-fixing and P-solubilizing bacteria, the result on agar media did not show antagonism in its growth. Bacillus pumilus and Bacillus megaterium effective at 5 % molasses medium with the number of 0.15 x 1012 on seven days of incubation.  
Characterization of protease-producing bacteria isolated from terasi Novi Arfarita; Ekachai Chukeatirote
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.549 KB) | DOI: 10.23869/89

Abstract

Total of 117 bacterial strains were isolated from terasi samples and 69% of isolates (71) could perform distinctive proteolytic activity that related to the ability to produce protease enzymes. Their proteolytic activity was further tested using spot incubation technique. Strain S4-5 has shown the highest activity then was selected for further tests in this study. Gram staining test showed that S4-5 is gram positive bacteria and able to grow under an aerobic condition. Based on API biochemical profiles, S4-5 strain bacteria was Bacillus licheniformis. Similarity test of genome sequence among Bacillusspecies from gene bank (EMBL Sequence Version) with Bacillusspp., strain S4-5 had similarity with Bacillus licheniformis genome. The optimal pH of this strain was 6 whereas the optimum temperature for Bacillus licheniformis strain S4-5 was 37°C.
Aplikasi Lapang Pupuk Hayati VP3 Dibandingkan Dengan Empat Macam Pupuk Hayati yang Beredar di Pasaran Terhadap Produksi Tanaman Kedelai (Glycine max L.) Putri Nur Azizah; S Sunawan; Novi Arfarita
Folium : Jurnal Ilmu Pertanian Vol 5, No 1 (2021): Folium : Jurnal Ilmu Pertanian
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/folium.v5i1.10359

Abstract

The study was undertaken to determine the effect of application VP3 biofertilizercompared to biofertilizers circulating in the market on the production of soybean (Glycinemax L.) in the field. The research was conducted on land in Bumi Asri Housing, DauDistrict, Malang Regency and the Applied Laboratory, Faculty of Agriculture, MalangIslamic University from June to October 2019. The research used a Randomized BlockDesign (RBD) with 6 treatments and was repeated 3 times. The results showed that theapplication of VP3 biofertilizer when compared with biofertilizers circulating in the marketin general gave better results to the growth and production variables of soybean in thefield. This was seen to be especially significant on the growth variable the leaf area perplant, total seed weight per plant, and total seed weight per plot.
Co-Authors adinda widya rahmawati Agus Sugianto Amanda Reha Zahran Anik Malikah Anis Rosyidah Anis Rosyidah Anton Muhibuddin aprilia dwi nastiti ayu azhariah Ayu Nur Setianingsih baltar ardyah pramesti Bambang Siswanto Bambang Siswanto Budi Prasetya Cahyo Prayogo, Cahyo Dhurrotul Roaidha Djuhari - Djuhari Djuhari Djuhari, Djuhari dwi nawang wulan eddrin wahyu febbiliani Ekachai Chukeatirote Endand Safitri, Endand erwin erwinn Feby Syaharani firman aprilianto dwi setiawan Firman Hidayat Hardadi Airlangga Hermawan Susanto hosinatul asror Indiyah Murwani Indiyah Murwani Indiyah Muwarni Indiyah Muwarni Khodiroh Shokibatun Mahayu Woro Lestari Maria Ulfah Mas'ut Khakim, Mohammat Masyhuri Masyhuri Miftahur Rohmah Mohammad Jasa Afroni nurhadiyanti rochmana Nurhidayati, Nurhidayati Nurlailah Nurlailah Prastyaji, Dimas Putri Nur Azizah Qur'ania, Anita rosita dwi S Sugiarto S Sunawan Sari, Sekar Arum Dwi Kartika sekar putri lisnanwati Shinta Dwi Cahya SITI MUSLIKAH Sugiarto , Sugiarto Sugiarto S Sugiarto Sugiarto Sunawan Sunawan Syafarotin Syafarotin Takaya Higuchi Takaya Higuchi, Takaya Tsuyoshi Imai Tsuyoshi Imai Tsuyoshi Imai Tsuyoshi Imai, Tsuyoshi wandi wandi Wiwit Nur Hidayah Yulia Nuraini Yuliana Yuliana