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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Innofarm : Jurnal Inovasi Pertanian Jurnal Ilmiah Inovasi
Suharjono Suharjono
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Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Other

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Blooming Stimulation of Microcystis in Sutami Reservoir Using Nutrients Nitrate and Phosphate in Different ratio Catur Retnaningdyah; Suharjono Suharjono; Agoes Soegianto; Bambang Irawan
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Microalgae Cyanobacteria from the genus Microcystis has the potential to bloom in Sutami reservoirs that located in Malang, Indonesia at a particular time. Microcystis is a notorious species because it can produce toxins that are dangerous to other organisms. The objective of this research is to determine the influence of different ratios of Nitrate and Phosphate on the growth of Microcystis in Sutami reservoir. The study was conducted from April to November 2009 and was carried out in situ in the reservoir Sutami with quasi experiment using a completely randomized design. Sutami reservoir water which already contained plankton community therein, including Microcystis were treated with five variations ratio of Nitrate and Phosphate (10, 20, 40, 80, and 160) and untreated water as control. Experiments carried out by using a transparent plastic bag diameter 1 m and 1.5 m long, inserted into the water and be immersed in the water by using bamboo. Calculation of Microcystis abundance and physic-chemical quality of water is done every three days for a month. The results showed that the higher concentration of orthophosphate in the media water of Sutami reservoir have the maximum abundance of Microcystis. Abundance of Microcystis grown in Sutami reservoirs have positively correlated with actual levels of nitrate, nitrite, total phosphate, conductivity, pH, temperature, ratio of Nitrate to Phosphate treatment and water KMnO4 value.Keywords: Blooming Microcystis, the ratio of nutrients Nitrate to Phosphate, Sutami reservoir
The Potency of Trichoderma sp. as A Biocontrol Agent against Fusarium sp. Pathogen of Porang (Amorphophallus muelleri Blume) Tuber Nindi Nazula Fajarini; Rodiyati Azrianingsih; Suharjono Suharjono
Journal of Tropical Life Science Vol. 10 No. 1 (2020)
Publisher : Journal of Tropical Life Science

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Abstract

Porang (Amorphophallus muelleri Blume) has high economic value in Asia. Porang tuber is high in glucomannan and thus becomes one of the exportable food commodities. Porang plants are susceptible to wilt disease caused by Fusarium sp. Trichoderma sp. has the ability to inhibit the growth of pathogenic fungi such as Fusarium sp. This research aimed to study the potency of Trichoderma sp. in controlling the growth of Fusarium sp. Trichoderma sp. was isolated from the rhizosphere soil in A. muelleri plantation, while Fusarium sp. was isolated from A. muelleri tuber infected by Fusarium. The fungi isolates were identified phylogenetically based on the similarity of Internal Transcribed Spacers (ITS) sequence. Both fungi were antagonistically assessed based on the dual culture method. The antagonistic assay showed that the two isolates of Trichoderma had the potency to inhibit the growth of the two isolates of Fusarium. Trichoderma sp. 2 has higher antagonistic potency than Trichoderma sp. 1. Based on ITS sequence similarity, Trichoderma sp. 1 and Trichoderma sp. 2 were identified as Trichoderma longipale and Trichoderma spirale respectively, while both pathogenic Fusarium were identified as Fusarium oxysporum. Trichoderma spirale therefore could be developed as a biopesticide agent in controlling Fusarium oxysporum.
APPLICATION OF CHAMPOR ON SEVERAL RICE SEED VARIETIES (Oryza sativa L.) AND THEIR EFFECTS ON MORTALITY IN SEED POWDER PESTS (Rizhopertha dominica: Kata kunci: Kapur barus,Varietas, Mortalitas, Rizhopertha dominica Fendy Nayogi; Suharjono Suharjono
Innofarm:Jurnal Inovasi Pertanian Vol. 24 No. 2 (2022): JURNAL INNOFARM : Jurnal Inovasi Petanian
Publisher : FAKULTAS PERTANIAN UNIVERSITAS SLAMET RIYADI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/innofarm.v24i2.7714

Abstract

This study aims to determine the effectiveness of champor with the active ingredient Naftalen on pest mortality Rizhopertha dominica seed powder pests and the effect of varieties on the intensity of attacks. The study was conducted using factorial completely randomized design (RAL) with 3 replications and 24 combinations. The first factor is the dose of camphor (C) consisting of 4 levels of C1 = 0 mg / 0.5 kg; C2 = 0.5 mg / 0.5 kg; C3 = 1 mg / 0.5 kg; C4 = 1.5 / 0.5 kg. The second factor is the type of variety (V) with 3 levels, namely V1 = Ciherang; V2 = Sintanur; V3 = Situbagendit. The results of the study, based on analysis of test Annova with the level of 5% stated that the application interaction with varieties of camphor significant effect on mortality Rizhopertha dominica. Further test results Duncan Multiple level of 5%, Aplication of camphor with the highest mortality values on C2 level of 0.5 mg / 0.5 kg of rice seeds was significantly different from the other dose. Varieties significantly affect the intensity of pest attacks warehouse dominica Rizhopertha seed powder, with a low intensity of the attack on the variety of different V3 Situbagendit real Ciherang varieties V1 and V2 Sintanur. Varieties significantly affect the intensity of pest attacks the best combination of each of the first factor and the second factor is Ciherang 0.5 mg / 0.5 kg (C2V1); Sintanur with a dose of 0.5 mg / 0.5 kg (C2V3); Situbagendit at a dose of 1 mg / 0.5 kg (C3V3).
Kandungan NPK Ekstrak Keong Mas (Pomacea canaliculata L.) yang Difermentasi Pada Beberapa Konsentrasi EM4 (Effective Microorganism 4): NPK Content of Golden Snail Extract Fermented at Some EM4 (Effective Microorganism 4) Concentrations Rahmawati; Sepdian Luri Asmono; Suharjono Suharjono; Nisa Budi Arifiana
Jurnal Ilmiah Inovasi Vol 24 No 2 (2024): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jii.v24i2.4444

Abstract

Penelitian ini bertujuan untuk mengetahui kandungan hara makro yaitu N-total, P2O5 dan K2O pada ekstrak keong mas yang difermentasi dengan menggunakan EM4 (Effective Microorganism 4). Penelitian ini dilakukan dengan cara menambahkan EM4 aktif yang terdiri dari 6 level konsentrasi (0%; 5%; 10%; 15%; 20%; 25%)  dan air gula ke dalam reaktor yang berisi limbah keong mas. Proses fermentasi dilakukakan selama 14 hari. Selanjutnya dilakukan pengambilan sampel pada hari ke 14 untuk dianalisis kandungan hara makro yang terdiri dari  P2O5 dan K2O dengan metode SNI 7763:2018 dan N-total dengan metode Kjeldhal (IKA-B.005). Hasil penelitian menunjukkan bahwa kadar N-Total dan Kalium berbeda sangat nyata, dimana terjadi penurunan kadar N total dari ekstrak keong mas pada beberapa konsentrasi EM4 aktif selama proses fermentasi, kandungan nitrogen paling tinggi terdeteksi pada fermentasi tanpa EM4 (0%), yaitu sebesar 1,766 % (v/v). Kadar Nitrogen paling kecil pada fermentasi menggunakan 25% EM4 aktif yaitu 1,474%. Sedangkan kandungan fosfor tidak mengalami penurunan kadar secara signifikan selama fermentasi 14 hari pada semua perlakuan.
Co-Authors Agoes Soegianto Catur Retnaningdyah Elfrida Ratnawati Fendy Nayogi Holyness Nurdin Singadimedja Nindi Nazula Fajarini Nisa Budi Arifiana Rahmawati Rahmawati Rodiyati Azrianingsih