Garuda - Garba Rujukan Digital

PENGARUH EKSTRAK DAUN BINTARO (Cerbera odollam) TERHADAP WAKTU BERHENTI MAKAN DAN MORTALITAS LARVA ULAT GRAYAK (Spodoptera litura) Turhadi Turhadi; Bedjo Bedjo; Suharjono Suharjono
Agro Bali : Agricultural Journal Vol 3, No 2 (2020)
Publisher : Universitas Panji Sakti

Efek negatif yang ditimbulkan oleh pestisida kimia terhadap ekosistem dan lingkungan mendorong usaha untuk menekan penggunaannya, salah satunya melalui penggunaan biopestisida nabati. Sebagai salah satu hama penting tanaman pertanian, ulat grayak (Spodoptera litura) perlu untuk ditekan populasinya untuk meminimalisir kehilangan hasil panen. Salah satu tanaman yang dapat dimanfaatkan sebagai biopestisida nabati untuk mengendalikan hama ulat grayak yaitu Cerbera odollam. Penelitian ini bertujuan untuk mengetahui potensi biopestisida nabati daun bintaro terhadap waktu berhenti makan (stop feeding) dan mortalitas larva ulat grayak. Penelitian ini didesain menggunakan rancangan acak lengkap yang terdiri dari enam taraf perlakuan (0 (kontrol), 10, 15, 20, 25, dan 30 g/L) dan masing-masing perlakuan diulang tiga kali. Ekstrak diaplikasikan ke larva ulat grayak dengan menggunakan metode leaf dipping methods. Efektivitas pengaruh ekstrak daun bintaro terhadap ulat grayak dilakukan dengan mengamati waktu berhenti makan (time of stop feeding) dan mortalitas (tingkat kematian larva). Hasil penelitian menunjukkan bahwa ekstrak daun bintaro berpotensi digunakan sebagai biopestisida nabati untuk larva S. litura. Mortalitas larva S. litura semakin meningkat sejalan dengan semakin lamanya waktu aplikasi ekstrak. Selain itu, mortalitas larva ulat grayak tertinggi terjadi pada perlakuan 20, 25, dan 30 g/L yaitu sebesar 40% pada 168 jam setelah aplikasi.

Komunitas Kapang Tanah di Lahan Kritis Berkapur DAS Brantas Pada Musim Kemarau Dian Siswanto; Suharjono Suharjono
Bioscientiae Vol 3, No 1 (2006): Bioscientiae Volume 3 No 1
Publisher : Universitas Lambung Mangkurat

The lime upland of Brantas River Watershed in East Java Province involve Malang, Blitar, Tulungagung and Trenggalek Regency are dry upland with limestone, low soil organic matter and unproductive. Soil productivity was influenced by microorganism activity that transform soil organic matter and other soil nutrition materials. Soil mold in lime upland of Brantas River Watershed can be used as bioindicator of productivity and soil sustainability. The research aims were to find out soil mold community at dry season in lime upland of The Brantas River Watershed and to know their relation with environmental factors. Chemical and physical parameters observed were soil temperature, sunlight intensity, soil acidity, soil moisture, soil organic matter and water retention capacity, while biological parameters was soil mold abundance. The result showed that soil mold abundances between groups were significantly different. The range of soil mold abundances in Trenggalek were 7x103-2.4x104 propaguls/gram, Tulungagung were 1.8x103-1.0x104 propaguls/gram, Blitar were 7.8x102-4.6x103 propaguls/gram and Malang were 2.8x102-9.0x103 propaguls/gram. Soil mold abundances were not significantly different between village in Malang Regency contain Pagak Village were 2.27x104 propaguls/gram, in Ngembul Village were 7.91x103 propaguls/gram and Banyuurip Village were 3.32x103 propaguls/gram. Soil mold abundances between regency and between village both were not significantly influenced for chemical physic factors. Predominant soil mold in Trenggalek and Blitar were Penicillium while Mucor were dominant in Tulungagung, Penicillium and Phytophtora were codominant in Malang.

Use of Macrozoobenthos for Water Quality Monitoring in Ecotourism Area of Prafi River, Manokwari, West Papua Sabarita Sinuraya; Endang Arisoesilaningsih; Suharjono Suharjono; Catur Retnaningdyah
Journal of Indonesian Tourism and Development Studies Vol. 6 No. 2 (2018)
Publisher : Program Pascasarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

The aim of research is to monitor the water quality of some ecotourism sites along Prafi Rivers (Manokwari, West Papua), especially in Subsay, SP1 reservoir and SP3 reservoir using macro-zoobenthos biotic index, Pratiâ€™s index and some chemical-physicalÂ factors of water. Research was done from July to September 2016. Sampling was performed through Purposive Random Sampling. Macro-zoobenthos was collected from each sampling sites in three locations, (upstream, middle stream, downstream) using Surber net and hand net. Water quality was categorized based on Implicit Pratiâ€™s Pollution index that is derived from DO, BOD5, pH, nitrate, orthophosphate and water temperature value. Macro-zoobenthos data was used to analyze the diversity index and six biotic indices. Result of the study showed that DO, BOD5, pH and nitrate value in all location meet water standard quality based on PP. no 82, 2001 class II (fisheries, recreation, animals husbandry and irrigation). The turbidity value in sites of upstream, middle stream and downstream Subsay and SP3 have meets standard water quality according to WHO for drinking water (<5 NTU). Based on the Pratiâ€™s index, the water quality was ranging from excellent to acceptable categories with value 0.4-1.9. The taxa richness of macro-zoobenthos was 31 taxa. The shannon-wiener diversity index in all stations were more than 2, indicated that the water was not polluted. The water quality of all station showed very excellent up to moderates (based on FBI), very excellent up to very bad (HBI), and excellent up to less polluted (ASPT). Percentage of Ephemeroptera, Plecoptera, and Trichoptera (% EPT) in all station up to 50% (excellent), except in station SP3 upstream (40%). EPT richness value in all station excellent to moderate. It can be concluded that the water quality of Subsay in upstream, middle stream, downstream and downstream of SP3 was excellent.The middle stream of SP3 and middle stream of SPI has moderate quality, while the water quality in SP3 upstream, SPI upstream and SPI downstream was bad.Keyword: Ecotourism, water quality, Prafi River.

Use of Macrozoobenthos for Water Quality Monitoring in Ecotourism Area of Prafi River, Manokwari, West Papua Sabarita Sinuraya; Endang Arisoesilaningsih; Suharjono Suharjono; Catur Retnaningdyah
Journal of Indonesian Tourism and Development Studies Vol. 6 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2018.006.02.05

The aim of research is to monitor the water quality of some ecotourism sites along Prafi Rivers (Manokwari, West Papua), especially in Subsay, SP1 reservoir and SP3 reservoir using macro-zoobenthos biotic index, PratiÃ¢'s index and some chemical-physical factors of water. Research was done from July to September 2016. Sampling was performed through Purposive Random Sampling. Macro-zoobenthos was collected from each sampling sites in three locations, (upstream, middle stream, downstream) using Surber net and hand net. Water quality was categorized based on Implicit Prati's Pollution index that is derived from DO, BOD5, pH, nitrate, orthophosphate and water temperature value. Macro-zoobenthos data was used to analyze the diversity index and six biotic indices. Result of the study showed that DO, BOD5, pH and nitrate value in all location meet water standard quality based on PP. no 82, 2001 class II (fisheries, recreation, animals husbandry and irrigation). The turbidity value in sites of upstream, middle stream and downstream Subsay and SP3 have meets standard water quality according to WHO for drinking water (<5 NTU). Based on the Prati's index, the water quality was ranging from excellent to acceptable categories with value 0.4-1.9. The taxa richness of macro-zoobenthos was 31 taxa. The shannon-wiener diversity index in all stations were more than 2, indicated that the water was not polluted. The water quality of all station showed very excellent up to moderates (based on FBI), very excellent up to very bad (HBI), and excellent up to less polluted (ASPT). Percentage of Ephemeroptera, Plecoptera, and Trichoptera (% EPT) in all station up to 50% (excellent), except in station SP3 upstream (40%). EPT richness value in all station excellent to moderate. It can be concluded that the water quality of Subsay in upstream, middle stream, downstream and downstream of SP3 was excellent.The middle stream of SP3 and middle stream of SPI has moderate quality, while the water quality in SP3 upstream, SPI upstream and SPI downstream was bad. Keyword: Ecotourism, water quality, Prafi River.

HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425193

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.

HA Aktivitas Antimikroba dan Identifikasi Gen Penyandi Enterocins isolat Enterococcus faecalis K2B1 dari Susu Kerbau Belang Toraja: Antimikroba dan Gen Penyandi Hasria Alang; Joni Kusnadi; Tri Ardyati; Suharjono Suharjono
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425193

Background: Enterocin in Enterococcus is coded by enterocin encoding genes namely A, B, P and L50A / B. The purpose of this study was to identify enterocin gene encoding enterococcus faecalis K2B1 probiotic candidate from Belang Toraja buffalo milk and antimicrobial activity to S. typhi. Methods: identification of enterocin gene encoding using ent A, B, P and L50A / B, partial purification using ammonium sulfate on 80 % concentration and antimicrobial activity against to Salmonella typhi using disk diffusion method. The results of PCR amplification are then sequenced and BLASTX on NCBI. Result: Antimicrobial activity of Precipitate and crude against S. typhi are 193 and 201 respectively. Identification gene encoding enterocin shows that Ent A, B and P cannot be amplified and only EntL50A / B can be amplified with a sequence size of 86 bp. The sequence of enterocin encoding genes in E. faecalis K2B1 has 94% similarity with hypothetical protein EB34_00789 E. faecalis on GenBank with accession number RBR60004.1 Conclusion: EntL50A / B E. faecalis K2B1 has a size of 86 bp and is 94% identical to the hypothetical protein EB34_00789 and Enterocin can be used as antimicrobial or bio preservative.

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search