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All Journal Journal of Dentistry Indonesia Majalah Kedokteran Gigi Indonesia Majalah Obat Tradisional Indonesian Journal of Cancer Dental Journal (Majalah Kedokteran Gigi) Journal of Dentomaxillofacial Science Jurnal Perikanan Universitas Gadjah Mada JURNAL CRANKSHAFT
Hendri Susanto
Department Of Oral Medicine, Faculty Of Dentistry, Gadjah Mada University, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Dentistry Energy Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health

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PENGARUH KONSENTRASI EKSTRAK ETANOL BONGGOL NANAS (Ananas comosus (L.) Merr) TERHADAP APOPTOSIS KARSINOMA SEL SKUAMOSA LIDAH MANUSIA Susanto, Hendri; Naritasari, Fimma; Supriatno, Supriatno
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.946 KB) | DOI: 10.14499/mot-TradMedJ15iss1pp%p

Abstract

Karsinoma  sel  skuamosa  lidah  merupakan  salah  satu  keganasan  yang sering terjadi di rongga mulut. Perawatan yang ada saat ini meliputi pembedahan, radioterapi, kemoterapi, maupun kombinasi ketiganya. Salah satu perawatan yang sedang  dikembangkan  antara  lain  adalah pencarian bahan alam/herbal yang dapat menginduksi  apoptosis  sel kanker.  Bromelain  memiliki  potensi  antikanker  salah  satunya  dengan menginduksi apoptosis. Bonggol nanas merupakan salah  satu bahan herbal  yang potensial  dikembangkan  untuk  perawatan  alternatif  karena  adanya  kandungan enzim  bromelain. Tujuan penelitian ini adalah  untuk mengetahui pengaruh konsentrasi ekstrak etanol bonggol nanas terhadap apoptosis biakan sel karsinoma skuamosa lidah manusia. Biakan  sel  karsinoma skuamosa  lidah manusia  diberi  perlakuan ekstrak  etanol  bonggol nanas tiga  konsentrasi  dibawah  nilai  IC50,  yang  diperoleh  dari  uji  sitotoksisitas,  yaitu konsentrasi 5.000, 5.500, dan 6.000 µg/ml. Pada uji apoptosis, setelah diinkubasi selama  24  jam,  sel  diwarnai  dengan  flurokrom  ethidium  bromide  dan  acridine orange. Pengamatan dan perhitungan dilakukan di bawah mikroskop flurescence. Sel  hidup  tercat  berwarna  hijau,  dan  sel  yang  mengalami  apoptosis  berwarna kuning  hingga oranye.  Analisis probit digunakan untuk menentukan nilai IC50 ekstrak etanol bonggol nanas dan Uji korelasi  pearson digunakan untuk mengetahui hubungan konsentrasi ekstrak etanol bonggol nanas dengan prosentase sel apoptosis. Hasil penelitian menunjukkan nilai IC50 esktrak etanol bonggol nanas pada biakan karsinoma sel skuamosa lidah. Penelitian ini juga menunjukkan terdapat hubungan positif antara konsentrasi ekstrak  etanol  bonggol  nanas  dan  apoptosis  (r  =  0,999,  p<0,05).  Kesimpulan  pada  penelitian  ini  adalah  bahwa  bonggol  nanas  mampu menginduksi  apoptosis  dan  terdapat  peningkatan  persentase  apoptosis  sel  yang sebanding dengan peningkatan konsentrasi ekstrak etanol bonggol nanas. 
Peran Ekstrak Etanol Kulit Manggis (Garcinia mangostana L.) Dalam Menginduksi Apoptosis Sel Kanker Lidah Manusia Sp-C1 In Vitro -, SUPRIATNO; SUSANTO, HENDRI; BUDIARTI, SRI
Indonesian Journal of Cancer Vol 7, No 4 (2013): Oct - Dec 2013
Publisher : "Dharmais" Cancer Center Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.115 KB)

Abstract

Sel kanker lidah mempunyai karakteristik pertumbuhan sel yang cepat, invasi, dan metastasis yang tinggi. Penatalaksanaan untuk kanker primer, metastasis, dan residif masih menunjukkan hasil yang belum memuaskan sehingga dipikirkan untuk mengombinasi dengan terapi pengobatan alternatif menggunakan bahan alam kulit manggis (Garcinia mangostana L.). Tujuan penelitian menguji induksi apoptosis sel kanker lidah Sp-C1 menggunakan ekstrak etanol kulit manggis in vitro. Induksi apoptosis sel pasca-perlakuan ekstrak etanol kulit manggis konsentrasi 0, 2,5, 5, 7,5, 10, dan 12,5 ?g/ml dilakukan menggunakan uji kolorimetrik caspase-3 dan -9 (DVED-pNA dan LEHD-pNA). Data dianalisis menggunakan Anova satu jalur, dilanjutkan dengan uji Post-hoc LSD dengan derajat kemaknaan 95%. Hasil penelitian menunjukkan ekstrak etanol kulit manggis konsentrasi 12,5 ?g/ml menginduksi apoptosis sel kanker lidah manusia Sp-C1 melalui aktivitas proteolitik caspase-3 dan caspase-9 (P=0,001). Peningkatan kelipatan aktivitas proteolitik caspase-3 dan -9 diketahui sebesar 1,39 dan 2,15 kali lipat. Kesimpulannya, ekstrak etanol kulit manggis dapat menginduksi apoptosis sel kanker lidah manusia Sp-C1.Kata Kunci: sel kanker lidah Sp-C1, kulit manggis, apoptosis, caspase-3 dan -9. 
RANCANG BANGUN MENARA REFLUKS PADA DESTILATOR BIOETHANOL KAPASITAS 5 LITER/JAM BERSKALA UMKM Susanto, Hendri; Winarso, Rochmad; Wibowo, Rianto
JURNAL CRANKSHAFT Vol 1, No 1 (2018): Jurnal Crankshaft
Publisher : Universitas Muria Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (845.363 KB) | DOI: 10.24176/crankshaft.v1i1.2588

Abstract

Bioethanol merupakan energi terbarukan yang berasal dari bahan dasar nabati. Sebagaimana fungsinya, bioethanol diharuskan memiliki tingkat kemurnian sesuai dengan standar mutu yang telah ditentukan sebagai energi terbarukan. Untuk mencapai keketentuan tersebut, telah dilakukan proses rancang bangun menara refluks pada destilator bioethanol pada saat proses kondensasi berlangsung. Metode yang digunakan pada penelitian ini adalah berupa studi literatur terhadap artikel terkait, proses perencanaan design, perancangan dan pembuatan terhadap komponen – komponen penyusun dari menara refluks, serta proses pengujian mesin untuk mengetahui fungsi kerja dari menara refluks pada destilator bioethanol. hasil penelitian menunjukkan menara refluks dapat digunakan sebagai proses kondensasi pada siklus destilasi bioethanol. Dengan dimensi shell berdiameter114 mm dan tinggi 2000 mm, dimensi tube berdiameter 94 mm dan tinggi total coil 400 mm dengan jarak antar coil 40 mm. dan dihasilkan kapasitas produksi ethanol pada mesin destilator bioethanol seabnyak 2,35 liter dengan kadar kemurnian 91%.
Unstimulated Salivary Flow Rate Corresponds with Severity of Xerostomia: Evaluation using Xerostomia Questionnaire and Groningen Radiotherapy-Induced Xerostomia Questionnaire Shinta, Friendika DAI.; Dinar, Nushita; Susanto, Hendri; Agustina, Dewi
Journal of Dentistry Indonesia Vol. 21, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of the oral complications in head and neck radiotherapy is xerostomia. The severity of xerostomia can be observed using objective examination (unstimulated salivary flow rate measurement) and subjective examination (assessment using xerostomia questionnaires). There are two questionnaires used in assessment of xerostomia in head and neck cancer radiotherapy namely Xerostomia Questionnaire (XQ) and Groningen Radiotherapy-Induced Xerostomia Questionnaire (GRIX). Objective: To know the correlation between unstimulated salivary flow rate and severity of xerostomia assessment using two questionnaires. Methods: 30 head and neck cancer patients undergoing radiotherapy at Radiotherapy Department of RSUP Dr. Sardjito Yogyakarta between January-April 2013 were involved in this study. The assessment of xerostomia used xerostomia questionnaires (XQ and GRIX). Unstimulated salivary flow rate was measured in ml/minutes. Data was analyzed using Spearman Rank Correlation. Results: There is a negative significant correlation between salivary flow rate and severity of xerostomia with correlation coefficient -0.452 (p
The correlation between serum zinc and vitamin D/25(OH)D in women with recurrent aphthous stomatitis Hendri Susanto; Puput Kendarwati; Sri Budiarti; Supriatno Supriatno
Majalah Kedokteran Gigi Indonesia Vol 5, No 2 (2019): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.42320

Abstract

Recurrent aphthous stomatitis (RAS) is an oral mucosal disease which was more prevalent in women than men. Study has shown that zinc deficiency associated with the occurance of RAS. Other study also have shown that vitamin D deficiency contributed in RAS. However, there is no study yet that reveal the association between serum zinc and vitamin D/25 (OH)D in women with RAS. To investigate the association between serum zinc and vitamin D/25(OH)D in women with RAS. Thirty two women patients with RAS who meet the inclusion criteria participated in this study. The inclusions criteria was non pregnant women who have been diagnosed RAS and did not have other oral diseases. The exclusions criteria were those who have systemic diseases, taking medications including multivitamin, and have bad habits (i.e. smoking). Data concerning characteristic of subjects, severity of RAS, serum vitamin D/25(OH)D, and zinc were collected and presented descriptively. The correlation between serum zinc and Vitamin D/25(OH)D was analyzed using Pearson correlation test with 95% confidence interval. This study has been approved by Medical and Health Ethics Committe, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta. All subjects of this study have low mean value of serum vitamin D/25(OH)D (11.08 ± 3.11 ng/mL) and categorized with vitamin D deficiency. The subjects also have low mean value of serum zinc (54.78 ± 9.19 μg/dL) and seventy percent subjects have mean value of serum zinc below normal. The result of Pearson correlation test showed that there was a significant positive correlation between mean serum zinc and Vitamin D/25(OH)D (r= 0.351, p<0.05) in women with RAS. Serum zinc associated with vitamin D/25(OH)D and play role in pathogenesis of RAS.
Herpes-associated erythema multiforme in a postmenopausal woman Hendri Susanto; Nurhilailah Nurhilailah; Raziv Ganesha; Hening Tuti Hendarti; Priyo Hadi
Majalah Kedokteran Gigi Indonesia Vol 7, No 1 (2021): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.43299

Abstract

Herpes-associated erythema multiforme (HAEM) is an acute inflammatory mucocutaneous disease which is characterized by ulcerative lesion on oral mucous membrane with or without skin involvements. The etiology of erythema multiforme (EM) is probably hypersensitivity reaction to human herpes simplex virus tipe 1 (HSV-1). Lesions on lips with HAEM can be treated by corticosteroid and acyclovir topically and/or orally. The objective of this study wasto report the case of HAEM with painful ulcers accompanying with crust on lips. A 55 years old woman complained painful ulcers with crust on lips since the last five months. She had visited a primary health care service and received several medications such as antibiotic (ciprofloxacin), anti-inflammatory drug (corticosteroid), analgesic (mefenamic acid) but her complaint was never healed completely because it was always recurring. The lesion on her lips got worse when she ate hot and spicy food and after she went out for outdor activities. She had a history of herpes virus infection seven months before but it was completely healed after treated by acyclovir topically and amoxicilin orally. An extra oral examination found black brown crust on her upper lip vermillion, well-demarcated, irregular margin, rough surface, erythematous surrounding area, accompanied with pain. An intra oral examination identified ulcers, yellowish, 4-5 mm in size, well-demarcated, irregular margin, redness in surronding area, accompanied with pain. Acomplete blood count examination, serology test for HSV-1 (IgM and IgG) were taken and the result showed that only IgG was reactive. The therapies given to the patient were topical medications with a combination of corticosteroid, chloramphenicol and moisturizer, followed by methyl prednisolone and acyclovir orally. After 6-week treatment, the lesions were completely healed.Herpes-associated erythema multiforme (HAEM) caused by hypersensitivity reactionto herpes simplex virus type 1 (HSV-1) may have a characteristic of painful ulcers accompanied with crust on the lips. The combination of both anti-inflammatory and antiviral drugs can be a choice for the therapy.
PENGARUH KONSENTRASI EKSTRAK ETANOL BONGGOL NANAS (Ananas comosus (L.) Merr) TERHADAP APOPTOSIS KARSINOMA SEL SKUAMOSA LIDAH MANUSIA Fimma Naritasari; Hendri Susanto; Supriatno Supriatno
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.946 KB) | DOI: 10.22146/tradmedj.8064

Abstract

Squamous cell carcinoma is one of the most common malignancy in the oral cavity.  Currently, cancer treatment using surgery, radiotherapy, chemotherapy and its combination.  New strategy for cancer therapy is by searching herbal which can induce apoptosis. Bromelain is one of a potential anticancer agent that can induce apoptosis. Pineapple stem is one of a potential herbal medicine because of its bromelain enzyme content. The aim of this study was to investigate the relation between pineapple stem ethanol extract and apoptosis of oral tongue squamous cell carcinoma cell line. Oral tongue  squamous  cell  carcinoma  cell  line was  treated  with  three concentrations  under  IC50  of  pineapple  stem  ethanol  extract,  including  5.000, 5.500,  6.000  µg/ml.  In  apoptosis  test,  cell  stained  with  fluorochrome  ethidium bromide and acridine orange after 24 hours incubation. Fluorescence microscope was used for counting the cell. Viable cell would be stained green and apoptotic cell would be stained yellow to orange. Probit analysis was used to determine IC50 of pineapple stem ethanol extract on Oral tongue squamous cell carcinoma cell line. Pearson analysis used to know the correlation between pineapple stem ethanol extract concentration and Oral tongue squamous  cell  carcinoma  cell  line apoptosis. The  result  showed  that  there  was  a  positive  correlation  between concentration  of  pineapple  stem  ethanol extract  and  apoptotic  cell  (r=0,999, p<0,05). In conclusion, pineapple stem ethanol extract treatment induced apoptosis of  oral  tongue  squamous  cell  carcinoma  and  the  increases  of  pineapple  stem ethanol extract concentration followed by the increase of apoptosis induction. 
Contact hypersensitivity after tongue piercing Ananta Herachakri P; Afrini Puspita; Feby Aryani; Hendri Susanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 3 (2010): September 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.811 KB) | DOI: 10.20473/j.djmkg.v43.i3.p126-130

Abstract

Background: Recently tongue piercing has become increasingly popular in the society. Several case reports have presented various complications of tongue piercing. However, there is no scientific evidence about contact hypersensitivity to tongue piercing. Purpose: The aim of this study was to investigate the contact hypersensitivity after using tongue piercing. Methods: Nineteen male Rattus norvegicus were divided into three groups: group A treated with vaseline on the back and dorsum tongue (control group), group B (I) treated with HgCl2 10% cream on the tongue dorsum, group B (II) treated with tongue piercing for 10 days and group C with HgCl 2 10% cream on the back, ear lobe, and tongue, then re-exposure with same materials on ear, back and tongue for 24 and 48 hours. Before and after 24 and 48 hours applications, ear width was measured with sliding caliper. At the end of treatments, the rats were sacrificed. All tissue specimens were made for Hematoylin Eosine (H&E) staining examination. The number of mononuclear cells was counted under light microscope Data was analyzed with One-Way ANOVA followed by LSD (p<0.05). Results: The result of this study showed that there were a significant difference of the thickness of ear lobe and the number of mononuclear cells (lymphocyte and monocyte) among all groups. Conclusion: It is concluded that tongue piercing induce contact hypersensitivity.Latar belakang: Saat ini pemakaian tongue piercing sangat popular di masyarakat. Beberapa laporan kasus menunjukkan bahwa tongue piercing menimbulkan beberapa komplikasi. Namun, belum ada bukti ilmiah mengenai reaksi hipesensitivitas tongue piercing. Tujuan: Untuk mengetahui reaksi hipersensitivitas setelah menggunakan tongue piercing. Metode: Sembilan belas tikus jantan Rattus novergicus yang dibagi dalam tiga kemlompok yaitu: grup A diberi perlakuan dengan vaselin pada punggung dan dorsum lidah, grup B (I) diberi perlakuan dengan krim HgCl2 10% pada dorsum lidah dan B (II) perlakuan tongue piercing selama 10 hari. Grup C diberi perlakuan dengan HgCl2 pada punggung, daun telinga, dan dorsum lidah, kemudian diberi perlakuan ulang dengan bahan dan tempat yang sama selama 24 dan 48 jam. Sebelum dan setelah perlakuan selama 24 dan 48 jam ketebalan telinga diukur dengan sliding caliper. Setalah perlakuan tikus didekapitasi kemudian dibuat preparat jaringan untuk pemriksaan hematoxilin & eosin (H & E). Perhitungan jumlah sel mononuclear dilakukan menggunakan mikroskop cahaya. Hasil: Penelitian ini menunjukkan bahwa terdapat perbedaan ketebalan telinga dan jumlah sel mononuklear yang bermakna setelah perlakuan antar kelompok pada hasil analisa dengan menggunakan ANOVA dan LSD (p<0.05). Kesimpulan: Tongue piercing dapat menginduksi reaksi hipersensitivitas kontak.
Xerostomia severity difference between elderly using alcohol and non-alcohol containing mouthwash Hendri Susanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 3 (2015): September 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.106 KB) | DOI: 10.20473/j.djmkg.v48.i3.p109-112

Abstract

Background: There are alcohol and non alcohol-containing mouthwash available in the market. Alcohol-containing mouthwash may have side effects which induced by alcohol in the mouthwash. Dry mouth/xerostomia may be a potential side effect of alcoholcontaining mouthwash when used by elderly person who has a tendency to have dry mouth. The evidence of  xerostomia induced by alcohol-containing mouthwash used by elderly is not available yet. Purpose: The aim of this study is to know the differences of xerostomia severity between elderly use alcohol-containing mouthwash and non alcohol-containing mouthwash. Method: This study was performed in elderly with age above sixty who do not have systemic diseases based on anamnesis, do not have oral diseases, and do not have allergy to one of  mouthwash components, do not use denture. Of total, thirty elderly participated in this study. The first group consists of elderly who use alcohol-containing mouthwash (AM) and the second group consists of elderly who use non alcoholcontaining mouthwash (NAM). Both groups use mouthwash for seven days (one week) twice a day. Xerostomia severity was assessed by VAS questionnaire. The mean score of the visual analogue score (VAS) xerostomia each group in day one (baseline) and day eight (post treatment) was analyzed by the Wilcoxon sign ranked test and Mann Whitney U test with 95% confidence level. Result: The VAS score of xerostomia post treatment (mean+SD/19.47+8.33) higher than baseline (mean+SD/15.87+8.91) in AM group (p<0.05), but, there is no significant difference of VAS score of xersotomia between post treatment (mean+SD/23.53+10.81) and baseline (mean+SD/23.67+11.82) in NAM group (p>0.05). The mean difference of VAS score of post-treatment and baseline between AM and NAM group was not significant (p>0.05). Conclusion: The conclusion is no significance difference of xerostomia severity between alcohol-containing mouthwash and non alcohol-containing mouthwash in elderly.
Periodontitis correlated with increased ESR and platelet counts in Indonesians with type 2 diabetes mellitus Hendri Susanto; Frank Abbas
Journal of Dentomaxillofacial Science Vol. 7 No. 1 (2022): (Available online: 1 April 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v7i1.1249

Abstract

Background : Periodontitis increases systemic inflammation in type 2 diabetes mellitus (DM2). Platelet count may be increased in periodontitis. The increased systemic inflammation and platelet counts are considered risk factors for atherosclerosis. However, there was no study investigating the correlation between periodontitis and platelet counts in type 2 diabetes mellitus in Indonesians. Objective: To assess the correlation between periodontitis, systemic inflammatory markers and platelet counts in Indonesian DM2 patients.   Methods: A full mouth periodontal examination including bleeding on probing, probing pocket depth, gingival recession, and plaque scores was performed in Indonesians DM2 in Dr. Sardjito Hospital, Yogyakarta. Indonesia. In addition, glycated hemoglobin (HbA1c), erythrocyte sedimentation rates (ESR), High sensitivity C reactive protein (Hs-CRP), peripheral blood counts, were assessed. To assess age, body mass index (BMI) education level, ethnicity, sex/gender, smoking, a standardized questionnaire was used and presented descriptively. In correlation and multiple linear regression analyses, it was assessed whether periodontitis operationalized with periodontal inflamed surface area (PISA) correlated with systemic inflammatory markers (Hs-CRP, ESR), HbA1c and peripheral blood counts (hemoglobin, leucocytes, erythrocytes, platelets, hematocrite) with 95% Confidence Interval.Results: In 44 Indonesians treated for DM2, PISA was associated with ESR (r = 0.32; p<0.05), and platelet count (r = 0.527; p < 0.05). Plaque score and PISA were the predictors for platelet count (p<0.05)Conclusion: Periodontitis Is associated with increased the systemic inflammation marker Erythrocyte Sedimentation Rate (ESR) and platelet counts in Indonesians DM2 and therefore may form an additional risk factor for atherosclerosis in these patients.
Co-Authors Afrini Puspita Afrini Puspita Ananta Herachakri Ananta Herachakri P Anne Handrini Dewi Deaoxi Renaschantika Djatumurti Dewi Agustina Feby Aryani Feby Aryani Fimma Naritasari Frank Abbas Hening Tuti Hendarti Heribertus Dedy Kusuma Yulianto Iriana, Fita Fathaya Nurhilailah Nurhilailah Nushita Dinar Priyo Hadi Puput Kendarwati Raziv Ganesha Rianto Wibowo. Rochmad Winarso Roissatun Nasikah Rosa Amalia Shinta, Friendika DAI. Sri Budiarti Sri Budiarti Poerwanto Sri Hadiati Supriatno Supriatno Supriatno Supriatno Supriatno Zipora Silka Yoretina