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Aktivitas Ekstrak Meniran (Phyllanthus niruri linn) Sebagai Immunostimulator pada Ayam yang Divaksin Penyakit Tetelo Jola Rahmahani; Rahaju Ernawati; Didik Handijatno
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Newcastle Disease or tetelo is one of main problem in poultry Industry in Indonesia. Prevention such as biosecurity control and routin vaccination program have been conducted to overcome this problem, but they have not given any great impact. Phyllanthus Niruri L. or meniran is well known as immunostimulatory. This research was aimed to reveal effect of Phyllanthus Niruri L. extract on chicken vaccinated with live vaccine LaSota. Administration of Phyllanthus Niruri L. extract was conducted on three different time which were 7 days before vaccination, 1 days after vaccination, and 3 days before and after vaccination. The amount of Phyllanthus Niruri L. extract administered were 2 ml, 2.5 ml, and 3 ml orally. Data of antibody titre were collected for 4 weeks after the treatment. It was obtained by measuring the antibody through Haemagglutination Inhibition test each week. According to the result Phyllanthus Niruri L. extract could increase the amount of antibody titre against Newcastle Disease. The amount of Phyllanthus Niruri L. extract given that capable to induced maximum of antibody titre was administered 1 days after the vaccination with amount 2.5 ml. It is suggested that Phyllanthus Niruri L. extract should be administered post vaccination to boost antibody synthesis.

Aktivitas Antimikrob Cuka Apel terhadap Multidrug Resistance Staphylococcus aureus yang Diisolasi dari Luka Infeksi Anjing di Surabaya Elisa Herina Dimariwu; Wiwiek Tyasningsih; Jola Rahmahani; Rahaju Ernawati; Mustofa Helmi Effendi; Didik Handijatno
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.917 KB)

Staphylococcus aureus is one of the normal flora that can cause infection in injured skin. Resistance to antibiotics has an impact on the difficulty of therapeutic treatment so that other alternatives are needed. The purpose of this study was to observe the effectiveness of apple vinegar as an antimicrobial against Multidrug Resistant Staphylococcus aureus isolated from infection wounds in dogs in Surabaya. The methods in this study were the isolation of bacteria from 30 samples of dog festering wounds on Manitol Salt Agar (MSA) media and identification through macroscopic, microscopic, catalase tests, coagulase tests, hemolysis tests on Blood Agar media, and Voges–Proskauer (VP) tests. Bacteria that have included the S. aureus criteria were followed by sensitivity tests to the antibiotics Amoxycillin, Ampicillin, Gentamicin, Chloramphenichol, and Ciprofloxacin. Apple vinegar activity test was carried out using disk diffusion method against Multidrug Resistant Staphylococcus aureus. The results showed that of the seven S. aureus isolates, there were two isolates belonging to the Multidrug Resistant S. aureus. The results of the apple vinegar activity test showed the presence of antimicrobial activity shown by the formation of a clear zone around the paper disk with an average diameter of 24.06 mm at a concentration of 90%. The conclusion shows that apple vinegar has antimicrobial activity against Multidrug Resistant S. aureus which is isolated from dog festering wounds in Surabaya.

Analisis Filogenetik Gen Hemaglutinin dan Neuraminidase Avian Influenza H9N2 Asal Ayam Petelur di Jawa Timur (PHYLOGENETIC ANALYSIS OF HAEMMAGLUTININ AND NEURAMINIDASE GENES OF AVIAN INFLUENZA H9N2 FROM LAYER INI EAST JAVA) Prestalia Dwi Rachmawati; Tatang Santanu Adikara; Hani Plumeriastuti; Rahaju Ernawati; Jola Rahmahani; Didik Handijatno; Christian Marco Hadi Nugroho
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.985 KB)

Avian influenza virus (AIV) subtype H9N2 is one of the infectious agents that threatens laying poultry farms, because it has an impact on drastically reducing production in the population. The aim of this study was to isolate and analyze phylogenetically the partial gene encoding the surface proteins of the AIV subtype H9N2 from laying hens in East Java. A total of 30 suspected AIV subtypes of H9N2 were taken from laying hens which had decreased production by up to 70% in three sub districts each in Kediri, Blitar and Tulung Agung regency, in East Java Province. The virus was isolated in embryonated chicken eggs and then followed by a Hemagglutination (HA) test. Detection of the presence of H9 and N2 genes was carried out through Reverse Transcriptase polymerase Chain Reaction (RT-PCR) and continued with partial gene sequencing of the two surface proteins. Data analysis was processed using BioEdit version 7.2.5 and Mega 7.0. The results show that only one sample, namely code B2.2 from Blitar regency, is an AIV subtype of H9N2. The virus in this study belong to clade h9.4.2.5 of the AIV subtype H9N2. The conclusion of this study is that the VAI subtype H9N2 was successfully isolated from laying hens in East Java and successfully identified phylogenetically.

Molecular characterization of Pasteurella multocida pfhaB1 gene fragment from buffalo and cattle isolates from Nusa Tenggara Timur Indonesia Ine Karni; Didik Handijatno; Lucia Tri Suwanti; Kusnoto Kusnoto; Jola Rahmahani; Wiwiek Tyasningsih
Aceh Journal of Animal Science Vol 4, No 2: December 2019
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.4.2.13581

Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida (P. multocida ) Serotypes B: 2. The fragment pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates. Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida; pfhaB1gene

UPAYA PENINGKATAN KESEHATAN MASYARAKAT MELALUI PENGETAHUAN MENDETEKSI FORMALIN PADA DAGING AYAM DI KELURAHAN JAMBANGAN SURABAYA Jola Rahmahani; Wiwiek Tyasningsih; A. T. Soelih Estoepangestie
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 2 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

The area of Surabaya Jambangan Village consists of 29 RTs and 7 RWs. The people of Surabaya, especially the Jambangan village, prefer to consume chicken meat compared to other meats, because in addition to containing high protein nutrients as well because the price is cheap so it is affordable by the public. Today many people have circulated in the community the use of formaldehyde in chicken meat for preservation so that meat is not easily decayed, but there are still many people who do not know the dangers of health. Community Service aims to empower the community, especially mothers of PKK members through the knowledge of detecting formaldehyde in chicken meat, which focuses on the knowledge of the community in recognizing formalin and the dangers to health. Community Service Results showed that there was an increase in understanding of PKK mothers in Jambangan Surabaya Village in recognizing formalin and the dangers to health and knowing how safe, healthy, whole and halal chicken meat (ASUH).AbstrakWilayah Kelurahan Jambangan Surabaya terdiri dari 29 RT dan 7 RW. Masyarakat Surabaya khususnya kelurahan Jambangan banyak memilih mengonsumsi daging ayam dibandingkan dengan daging yang lain, karena selain mengandung gizi yang tinggi protein juga karena harganya murah sehingga terjangkau oleh masyarakat. Dewasa ini telah banyak beredar di masyarakat adanya penggunaan formalin pada daging ayam untuk pengawetan supaya daging tidak mudah busuk, namun masih banyak masyarakat yang belum mengetahui bahayanya terhadap kesehatan. Pengabdian kepada Masyarakat ini bertujuan untuk memberdayakan masyarakat khususnya ibu-ibu anggota PKK melalui pengetahuan mendeteksi formalin pada daging ayam, yang difokuskan pada pengetahuan masyarakat dalam mengenal formalin dan bahayanya terhadap kesehatan. Hasil Pengabdian kepada Masyarakat menunjukkan bahwa ada peningkatan pemahaman ibu-ibu PKK di Kelurahan Jambangan Surabaya didalam mengenal formalin dan bahayanya terhadap kesehatan serta mengenal bagaimana daging ayam yang aman, sehat, utuh dan halal (ASUH).

PENYULUHAN MASTITIS SUBKLINIS DAN KEGIATAN UJI CALIFORNIA MASTITIS TEST PADA SAPI PERAH DI KOPERASI UNIT DESA KECAMATAN NGANCAR, KABUPATEN KEDIRI, JAWA TIMUR Yulianna Puspitasari; Wiwiek Tyasningsih; Jola Rahmahani; Suwarno Suwarno; Sri Chusniati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 5 No. 2 (2021): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v5i2.2021.489-495

AbstractSubclinical mastitis is an inflammation of the udder that often attacks livestock, especially milk-producing livestock. The impact of the subclinical mastitis losses is quite large, including a drastic decrease in the quantity and quality of milk, but no pathological changes were found in cows. The Karya Bhakti Village Unit Cooperative (VUC) is a center for milk production in the Kediri Regency which accommodates dairy cows' milk products from people's farms in the Kediri Regency. The low awareness of farmers in the management of livestock health care and hygiene results in low productivity and quality of milk and the high incidence of subclinical mastitis. This encourages counseling about subclinical mastitis. The socialization method used for counseling were carried out by presenting material about subclinical mastitis with lecture techniques (power point slides), videos about the method of early detection of subclinical mastitis using California Mastitis Test (CMT), conducting demonstrations of testing milk samples using the CMT method and discussion about the material that has been delivered as an indicator of the success of counseling. This series of counseling activities is an effort to reduce the incidence of subclinical mastitis through increasing public knowledge and understanding. This activity also has a positive impact by reducing infectious diseases, especially subclinical mastitis in livestock so that the health of livestock and products from livestock will also be guaranteed both in terms of quality and quantity.Keywords: mastitis, subclinical mastitis, cows, Counseling, CMTAbstrakMastitis subklinis merupakan radang ambing yang sering menyerang hewan ternak khususnya ternak penghasil susu. Dampak kerugian mastitis subklinis diantaranya penurunan kuantitas dan kualitas susu secara drastis, namun tidak ditemukan adanya perubahan patologis pada sapi. Koperasi Unit Desa (KUD) Karya Bhakti merupakan KUD yang menjadi sentra penghasil susu di Wilayah Kabupaten Kediri yang menampung hasil susu sapi perah dari peternakan – peternakan rakyat di Wilayah Kabupaten Kediri. Rendahnya kesadaran peternak dalam manajemen kesehatan ternak dan hiegienitas kandang mengakibatkan rendahnya produktivitas dan kualitas susu serta masih tingginya angka kejadian mastitis subklinis. Angka kejadian mastitis subklinis yang tinggi ini mendorong untuk dilakukan penyuluhan mengenai mastitis subklinis. Metode sosialisasi yang digunakan untuk penyuluhan adalah menggunakan alat peraga berupa slide power point yang berisikan materi mastitis subklinis, video metode deteksi dini mastitis subklinis menggunakan California Mastitis Test(CMT), demonstrasi CMT pada sampel susu serta tanya jawab seputar materi yang telah disampaikan sebagai salah satu indikator keberhasilan penyuluhan. Rangkaian kegiatan penyuluhan ini sebagai upaya untuk menekan angka kejadian mastitis subklinis melalui peningkatan pengetahuan dan pemahaman masyarakat. Kegiatan ini juga memberikan dampak positif dengan menurunnya penyakit infeksi khususnya mastitis subklinis pada hewan ternak sehingga kesehatan hewan ternak dan produk asal hewan ternak juga akan terjamin baik dari segi kualitas maupun kuantitasnya.Kata Kunci: mastitis, mastitis subklinis, sapi perah, penyuluhan, CMT

Application of Spa-Gene as A Marker of Molecular Epidemiology in the Case of Methicillin-Resistant Staphylococcus Aureus (MRSA) Which its from Dairy Cows in Surabaya Region Asri Rizky; Mustofa Helmi Effendi; Jola Rahmahani
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cow’s milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cow’s milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain.

Application of Spa-Gene as A Marker of Molecular Epidemiology in the Case of Methicillin-Resistant Staphylococcus Aureus (MRSA) Which its from Dairy Cows in Surabaya Region Asri Rizky; Mustofa Helmi Effendi; Jola Rahmahani
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.4817

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cow’s milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cow’s milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain.

Penambahan Suplemen Madu dalam Pakan Guna Meningkatkan Pertumbuhan Dan Kelulushidupan Benih Lobster Air Tawar Red Claw (Cherax quadricarinatus) [The Addition Supplement Of Honey In Feed To Increasing Growth And Survival Rate Of Freshwater Crayfish Seed Red Claw (Cherax quadricarinatus) ] Akhmad Taufiq Mukti; Fachriza Tri Aprilia; Jola Rahmahani; Muhammad Arief
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11644

Abstract The demand for domestic or worldwide market to freshwater crayfish is high. Wherefor, needed some cultivation to reach that demand. The growth period of freshwater crayfish is slowly, then to increase growth needed addition some supplement in the feed. The aim of this research was to find out the influence of addition honey in the feed concerning to growth and survival rate freshwater crayfish seed red claw (C. quadricarinatus) and also to figure out the best dose of addition honey which one could increase growth and survival rate of freshwater crayfish red claw (C. quadricarinatus) seed. The method in the research was used Completely Randomized Design with 9 treatment. The addition doze of honey in every feed is different such as A (0 ml/kg of feed) as control, B (300 ml/kg of feed), C (400 ml/kg of woof), D (500 ml/kg of feed), E (600 ml/kg of feed), F (700 ml/kg of feed), G (800 ml/kg of feed), H (900 ml/kg of feed) dan I (1000 ml/kg of feed), in every treatment must repeated 3 times. The result of this research figure out that an addition honey in feed of freshwater crayfish red claw (C. quadricarinatus) was not obviously influence to growth and also survival rate. The best doze of honey to add in feed of freshwater crayfish red claw (C. quadricarinatus) to reach maximal weight growing up is 800 ml/kg of feed.

Evaluasi Penyuluhan Penyakit Infeksius dan Reproduksi Ternak di Blitar Raya Suwarno Suwarno; Erma Safitri; Jola Rahmahani; Kadek Rahmawati; Supriyadi Supriyadi; Gabriel Sampe Pasang; Dyah Irfani; Pitaloka Kurniasari
Bima Abdi: Jurnal Pengabdian Masyarakat Vol. 5 No. 2 (2025): Bima Abdi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Pendidikan Bima Berilmu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53299/bajpm.v5i2.1578

Blitar Raya (Kota dan Kabupaten) merupakan salah satu kantong ternak di Jawa Timur. Merebaknya kasus PMK (Penyakit Mulut dan Kuku) dan LSD (Lumpy Skin Disease) menyebabkan turunnya populasi ternak. Kasus yang dihadapi pemerintah setempat masih adanya kasus PMK dan LSD meskipun vaksinasi dan biosekuriti sudah dilakukan. Dampak lain akibat kedua jenis penyakit tersebut adalah menurunnya produksi susu dan gangguan reproduksi. Pengabdian kepada Masyarakat ini dilakukan untuk memberikan penyuluhan terkait materi tentang Virologi (program vaksinasi dan biosekuriti) dan Reproduksi ternak (deteksi birahi dan repeat breeding). Peserta kegiatan ini adalah para Dokter Hewan dan Paramedis yang ada di wilayah Blitar Raya. Ceramah dan diskusi dilakukan dengan mengundang para peserta untuk mengikuti kegiatan penyuluhan di ruang pertemuan RM Bu Mamik Kota Blitar. Materi disajikan dalam bentuk power point menggunakan LCD. Di akhir diskusi peserta diberikaan soal dalam bentuk kuesioner, dengan topik materi tentang Virologi dan Reproduksi. Topik materi Virologi dibagi lagi menjadi subtopik vaksinasi dan biosekuriti, sedangkan topik materi Reproduksi dibagi menjadi subtopik deteksi birahi dan repeat breeding. Penilaian kuesiononer didasarkan atas jawaban benar-salah dan kemampuan peserta di dalam menganalisis topik dan subtopik yang diberikan. Penilaian didasarkan atas pengetahuan peserta beda strata pendidikan terhadap materi yang diujikan. Kesimpulan dari hasil penilaian adalah strata pendidikan dan pengalaman lapangan memiliki peran penting di dalam menguasai topik materi penyuluhan.

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