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All Journal Current Biochemistry Jurnal Pangan dan Agroindustri MITRA: Jurnal Pemberdayaan Masyarakat Jurnal Ilmu Pengetahuan dan Teknologi (IPTEK) Jurnal Teknologi Lingkungan
Shinta Leonita
Institut Teknologi Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Industrial & Manufacturing Engineering Public Health

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Isolation and Identification of Endophytic Bacteria from Ficus variegata Blume as Antibacterial Compounds Producer Shinta Leonita; Maria Bintang; Fachriyan Hasmi Pasaribu
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Endophytic bacteria can produce antibacterial compounds. Their existence in the medicinal plant of Ficus variegata Blume enables the production of bioactive compounds similar to those contained by the host plants. The purpose of this study was to isolate and identify endophytic bacteria from F. variegata which is potential to produce antibacterial compounds. The methods used include isolation of endophytic bacteria from leaves, stem, aerial root, and fruit of F. variegata plants. Antibacterial activity assay was done against four types of bacteria i.e. Staphylococcus aureus, Bacillus subtillis, Escherichia coli, and Psedomonas aeruginosa. Identification of endophytic bacteria was conducted based on morphological analysis, biochemical test, and molecular analysis of 16S rRNA. Endophytic bacterial culture was extracted by ethyl acetate and analyzed by GC-MS. A total of 29 isolates of endophytic bacteria were obtained from F. variegata. The BH2 isolate was found to have potential activity. Analysis of 16S rRNA showed that BH2 isolate was related to Pseudomonas aeruginosa strain SV1 with 99 % identity. The result of GC-MS analysis showed that the antibacterial compound was Nonanoic acid ethyl ester
Aktivitas Antibakteri Isolat Bakteri Endofit dari Tumbuhan Nyawai (Ficus variegata Blume) Shinta Leonita; Maria Bintang; Fachriyan Hasmi Pasaribu
JURNAL ILMU PENGETAHUAN DAN TEKNOLOGI (IPTEK) Vol. 2 No. 1 (2018): Jurnal Ilmu Pengetahuan dan Teknologi (IPTEK)
Publisher : Institut Teknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31543/jii.v2i1.133

Abstract

Bakteri endofit menjadi salah satu alternatif penghasil senyawa antibakteri. Keberadaannya pada tumbuhan berkhasiat obat salah satunya tumbuhan nyawai (Ficus variegata Blume) memungkinkan memproduksi senyawa bioaktif seperti yang terkandung oleh tumbuhan inangnya. Ekstraksi senyawa bioaktif dari tanaman dinilai tidak efisien sebab memerlukan biomassa yang besar, sehingga  cara yang dapat digunakan adalah dengan memanfaatkan bakteri endofit. Penelitian ini bertujuan mengisolasi dan menguji aktivitas antibakteri isolat bakteri endofit dari tumbuhan nyawai terhadap Eschericia coli, Staphylococcus aureus, Bacillus subtilis dan Pseudomonas aureus. Sebanyak 29 isolat bakteri endofit berhasil diisolasi dari tumbuhan nyawai. Berdasarkan uji antibakteri sebanyak 5 isolat bakteri endofit mampu menghambat pertumbuhan E. Coli, 6 isolat mampu menghambat S. aureus, 4 isolat mampu menghambat B. subtilis dan 4 isolat mampu menghambat P. aeruginosa. Kata Kunci : antibakteri, bakteri endofit, Ficus variegata Blume
Analisis Kelayakan Tekno-Ekonomi Produk Agroindustri Kacang Lurik Sangrai di Kota Tangerang Selatan Shinta Leonita; Graha Djustika Marsudi Harta; Annuridya Rosyidta Pratiwi Octasylva; Heru Irianto
JURNAL ILMU PENGETAHUAN DAN TEKNOLOGI (IPTEK) Vol. 4 No. 1 (2020): Jurnal Ilmu Pengetahuan dan Teknologi (IPTEK)
Publisher : Institut Teknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31543/jii.v4i1.156

Abstract

Salah satu produk olahan kacang tanah yang cukup popular di Kota Tangerang Selatan adalah kacang sangrai.  Kacang tanah varietas lurik (Arachis Hypogaea L. ‘Lurik’ ) adalah kacang tanah unggul yang memiliki karakteristik berbeda dari  jenis kacang tanah pada umummnya dan berpotensi untuk dijadikan sebagai bahan baku pada agroindustri kacang sangrai di Kota Tangerang Selatan. Penelitian ini bertujuan untuk menganalisis teknoekonomi industri pengolahan kacang lurik sangrai. Metode penelitian yang digunakan adalah wawancara dan observasi, studi pustaka untuk mempelajari deskripsi produk, serta penelitian pengembangan industri kacang sangrai yang meliputi analisis produksi, analisis manajemen dan organisasi, analisis finansial, analisis sensitivitas, serta desain kemasan produk. Hasil penelitian diperoleh bahwa usaha kacang Lurik sangrai layak dijalankan dengan nilai investasi sebesar Rp520.095.000, biaya variabel Rp.1.638.128.668, kapasitas produksi 220 kemasan dengan netto 500 g, harga jual Rp. 29.908/kemasan, dan analisis usaha diperoleh nilai break even point 59.081/tahun, pay back period 1.23 tahun, net present value Rp. 1.097.788.068, net benefit cost ratio 1,34, internal rate of return 49,42%, dengan kapasitas produksi 405.594 kg per tahun, serta teknologi proses pengolahan kacang Lurik sangrai harus dilakukan sortasi bahan baku dan sortasi produk jadi agar menghasilkan produk kacang sangrai dengan karakteristik mutu yang baik.  
The Production of Roasted Peanuts Using the Semi-Mechanical Roaster in Keranggan Village Shinta Leonita; Abu Amar; Setiarti Sukotjo; Heru Irianto
MITRA: Jurnal Pemberdayaan Masyarakat Vol 6 No 1 (2022): MITRA: Jurnal Pemberdayaan Masyarakat
Publisher : Institute for Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/mitra.v6i1.3049

Abstract

Roasted peanuts typically have a unique taste with a distinctive roasted aroma and a savory flavor different from peanuts processed using an oven. To make roasted peanuts, two critical stages should be observed: the sanitation during washing and the roasting technology. The current study focuses on the application of roasting technology in the production of roasted peanuts among the households in Keranggan Village, Setu District, South Tangerang City. It was an issue that the household scale manufacturing of roasted peanuts there did not meet the standard process, relying on manpower to monitor when the roasting process should end even though fatigued workers may produce burnt peanuts up to 19%. Therefore, to reduce the percentage of burnt peanuts, a semi-mechanical roasted fryer was developed for the roasted peanut entrepreneurs in Keranggan Village, Setu District, South Tangerang City. The research team conducted socialization of effective roasting technology, provided assistance, and delivered the semi-mechanical roasters to the community. The team also performed a direct test on the semi-mechanical roaster at both the Indonesian Institute of Technology laboratory and Keranggan Village, in addition to providing assistance on how to use the tool. The semi-mechanical roaster itself is made of stainless steel with a diameter of 100 cm, equipped with a stirrer powered by an electric motor capable of roasting automatically within a certain time limit. This will produce a homogeneous heat transfer with the sand media, which is not changed in line with the original roasting method. The lab and field trials showed that the burnt yield decreased to 5%. Furthermore, when compared to those produced through manual roasting, the sensory test of the products, showed results that were not significantly different even with a sensory value of appearance (i.e., the color) higher than that of manual roasting.
AMINO ACID PROFILE ANALYSIS OF RED AND GREEN MELINJO PEELS TEA Saragih, Raskita; Tamizi, Ermiziar; Leonita, Shinta
Jurnal Pangan dan Agroindustri Vol. 9 No. 4: October 2021
Publisher : Department of Food Science and Biotechnology, Faculty of Agriculture Technology, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpa.2021.009.04.2

Abstract

       The study aims to analyze the profile of non-essential and essential amino acids in the peels and herbal tea products made from red and green melinjo peels. The processing of melinjo seeds into chips in  Pandeglang Regency in Banten Provence, produces large amounts of melinjo peels waste.  The processed tea from red and green melinjo peels contains polyphenol compounds, antioxidants, protein and amino acids that are good for health. The processing stages of the melinjo peel tea by sorting the peels for red and green melinjo peels, then washed, made them into thin slices, and dried using an oven blower at 65oC of temperature for 4 hours. Both green and red melinjo peels tea were analyzed for the amino acid profile using the UPLC method. Based on the results of the analysis, it was found that the amino acid content of melinjo peels and melinjo peels tea, both green and red, consisted of 7 non-essential amino acids and 8 essential amino acids. L-glutamic acid and L-aspartic acid are the highest amino acid components which can give melinjo peels tea a characteristic aroma and taste.
Evaluation of the Biophysical and Chemical Conditions of the Aquatic Environment During the Rainy Season to Mitigate the Ecological Disaster of Ciguatera Fish Poisoning (CFP) and Harmful Algal Bloom (HABs) in the Gili Matra Marine Tourism Park-Lombok: Evaluasi Kondisi Biofisikakimia Lingkungan Perairan Pada Musim Hujan Untuk Mitigasi Bencana Ekologi Ciguatera Fish Poisoning (CFP) dan Marak Alga Berbahaya (MAB) di Taman Wisata Perairan Gili Matra-Lombok Sachoemar, Suhendar Indrakusmaya; Rachman, Arief; Aliah, Ratu Siti; Megawati, Novi; Romadhona, Ekky Ilham; Meirinawati, Hanny; Prayogo, Teguh; Dewa, Riardi Pratista; Zamroni, Yuliadi; Ahyadi, Hilman; Fitriya, Nurul; Hamzah, Faisal; Garno, Yudhi Soetrisno; Susanto, Joko Prayitno; Riyadi, Agung; Haryanti; Ihsan, Iif Miftahul; Setiarti Sukotjo; Iskandar; Leonita, Shinta; Diswandi; Rahman; Muhami; Makosim, Syahril
Jurnal Teknologi Lingkungan Vol. 26 No. 2 (2025)
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jtl.2025.7213

Abstract

Abstrak Ciguatera Fish Poisoning (CFP) dan fenomena Marak Alga Berbahaya (MAB) yang disebabkan oleh dinoflagellata bentik beracun merupakan salah satu bencana ekologi atau lingkungan yang dapat mengancam kesehatan manusia di kawasan pesisir Indonesia, seperti di kawasan Taman Wisata Perairan (TWP) Gili Matra yang terdiri dari 3 pulau yaitu Gili Meno, Air, dan Trawangan. TWP Gili Matra merupakan pusat wisata bahari dan kawasan konservasi laut di kawasan Nusa Tenggara Barat yang diketahui mengalami tekanan akibat aktivitas manusia dari kegiatan pariwisata dan perikanan yang berdampak negatif kepada ekosistem pesisir, terutama terumbu karang. Kondisi tersebut dapat meningkatkan kelimpahan komunitas dinoflagellata bentik beracun yang berpotensi menyebabkan terjadinya CFP dan MAB di TWP Gili Matra. Untuk itu diperlukan upaya mitigasinya agar risiko bencana ekologi CFP dan MAB dikemudian hari yang selain dapat menyebabkan terjadinya kerusakan ekosistem perairan TWP Gili Matra, tetapi juga kerugian ekonomi yang cukup besar, tidak terjadi. Menurut perhitungan ekonomi para ahli, nilai ekonomi kegiatan pariwisata di TWP Gili Matra, mencapai 26,86 triliun rupiah per tahun.  Salah satu upaya mitigasi bencana ekologi CFP dan MAB adalah dengan mengetahui kondisi lingkungan perairan di TWP Gili Matra pada musim hujan. Berdasarkan hasil survei diketahui kondisi biofisikakimia lingkungan perairan pada musim hujan bulan Desember 2022 dan Maret 2024 dalam keadaan yang sangat baik dengan tingkat eutrofikasi sebagai indikator kualitas lingkungan perairan pada tingkat ultraoligotrofik dengan nilai skor TSI (Trophic State Index) 12,9-19,4 (<30) yang mengindikasikan bahwa kesuburan perairan sangat rendah, air jernih, konsentrasi oksigen terlarut tinggi sepanjang tahun dan mencapai zona hipolimnion.   Abstract Ciguatera Fish Poisoning (CFP) and the phenomenon of Harmful Algal Bloom (HABs) caused by toxic benthic dinoflagellates are one of the ecological or environmental disasters that can threaten human health in coastal areas of Indonesia, such as in the Gili Matra Aquatic Tourism Park (ATP) area which consists of three islands, namely Gili Meno, Air, and Trawangan. ATP Gili Matra is a center for marine tourism and conservation areas in the West Nusa Tenggara region which is known to be under pressure due to human activities from tourism and fisheries activities that have a negative impact on coastal ecosystems, especially coral reefs. This condition can increase the abundance of toxic benthic dinoflagellate communities that have the potential to cause CFP and HABs in ATP Gili Matra. For this reason, mitigation efforts are needed so the risk of CFP and HABs ecological disasters in the future which can not only cause damage to the ATP Gili Matra aquatic ecosystem, but also significant economic losses, does not occur. According to economic calculations by experts, the economic value of tourism activities in ATP Gili Matra reaches IDR 26.86 trillion per year. One of the efforts to mitigate the ecological disaster of CFP and HABs is to understand the condition of the aquatic environment in ATP Gili Matra during the rainy season. Based on the survey results, it is known that the biophysical and chemical conditions of the aquatic environment during the rainy season in December 2022 and March 2024 are in very good condition with the level of eutrophication as an indicator of the quality of the aquatic environment at the ultraoligotrophic level with a TSI (Trophic State Index) score of 12.9-19.4 (<30) which indicates that water fertility is very low, the water is clear, the concentration of dissolved oxygen is high throughout the year and reaches the hypolimnion zone.
Co-Authors Abu Amar Agung Riyadi Annuridya Rosyidta Pratiwi Octasylva Arief Rachman Diswandi Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faisal Hamzah Garno, Yudhi Soetrisno Graha Djustika Marsudi Harta Haryanti Heru Irianto Heru Irianto Hilman Ahyadi Ihsan, Iif Miftahul ISKANDAR Joko Prayitno Susanto Makosim, Syahril MARIA BINTANG Megawati, Novi Meirinawati, Hanny Muhami Nurul Fitriya Rahman Raskita Saragih Ratu Siti Aliah Riardi Pratista Dewa, Riardi Pratista Romadhona, Ekky Ilham Sachoemar, Suhendar Indrakusmaya Setiarti Sukotjo Tamizi, Ermiziar Teguh Prayogo Yuliadi Zamroni