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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Layanan Masyarakat (Journal of Public Service) Sains dan Matematika Berkala Penelitian Hayati
Salamun Salamun
Departemen Biologi, Fakultas Sains Dan Teknologi, Universitas Airlangga Kampus C Unair, Jln. Mulyorejo Surabaya 60115
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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PELATIHAN PENGOLAHAN LIMBAH TOILET MENGGUNAKAN FORMULA MIKROBA DI PERUMAHAN TENGGILIS SURABAYA Fatimah Fatimah; Tini Surtiningsih; Agus Supriyanto; Nima’tuzahroh Nima’tuzahroh; Salamun Salamun; Tri Nurhariyati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1121.164 KB) | DOI: 10.20473/jlm.v2i1.2018.25-29

Abstract

Blockage of septic tanks is one of the problems in the community related to the discharge of toilet waste produced. The use of synthetic toilet cleaners that are not environmentally friendly is one of the factors that can inhibit the process of decomposing organic waste. The existence of microbial killer ingredients in toilet cleaning materials that come into the septic tank will actually inhibit the growth of essential microbes that play a role in the perfection of the decomposition process. Lack of understanding of the community related to microbial processes in the processing of toilet waste is one of the factors inability of the community to overcome these problems. This community service aims to disseminate information related to the processing of organic waste, the role of microbes in the processing of toilet waste, how to make microbial formulas that play a role in the processing of toilet waste, as well as efforts to increase the creativity of the community to process their own waste to improve environmental sanitation and improve community welfare. The results of the training showed that the average pretest and post test scores of the cadres of Tenggilis Surabaya village experienced a significant increase in value with a mean value of 37.78 (pretest) to 62.22 (post-test). Toilet waste processing cadres have been able to make biotoilet products that can be used to process limbat toilets in their homes. The informationand insights they receive can be passed on to the community from each cadre.AbstrakPenyumbatan septic tank merupakan salah satu permasalahan di masyarakat terkait dengan buangan limbah toilet yang dihasilkan. Penggunaan pembersih toilet yang berbahan sintetik yang tidak ramah lingkungan merupakan salah satu faktor yang dapat menghambat proses penguraian limbah organik. Keberadaan bahan pembunuh mikroba dalam bahan-bahan pembersih toilet yang ikut masuk ke dalam septik tank justru akan menghambat pertumbuhan mikroba esensial yang berperan dalam kesempurnaan proses dekomposisi. Kurangnya pemahaman masyarakat terkait dengan proses mikroba dalam pengolahan limbah toilet merupakan salah satu faktor ketidakmampuan masyarakat dalam mengatasi permasalahan tersebut. Pengabdian masyarakat ini bertujuanuntuk mensosialisasikan informasi terkait proses pengolahan limbah organik, peran mikroba dalam pengolahan limbah toilet, cara membuat formula mikroba yang berperan dalam pengolahan limbah toilet, serta upaya meningkatkan kreativitas masyarakat untuk mengolah limbahnya sendiri guna meningkatkan sanitasi lingkungan dan meningkatkan kesejahteraan masyarakat. Hasil pelatihan menunjukkan bahwa rerata nilai pretest dan post test kader kelurahan Tenggilis Surabaya mengalami peningkatan nilai yang cukup signifi kan dengan rerata nilai dari 37,78 (pretest) menjadi 62,22 (post test). Kader pengolahan limbah toilet telah mampu membuat produk biotoilet yang dapat dimanfaatkan untuk mengolah limbat toilet di rumah masing-masing. Informasi dan wawasan yang mereka terima dapat di teruskan kepada komunitas asal masing-masing kader.
TOKSISITAS Bacillus sphaericus H-5a5b (VCRC B42i) TERHADAP LARVA Culex quinquefasciatus Salamun; Rosmanida; Ni’matuzzahroh; R.A. Samsumaharto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 2 No 2 (1996): December 1996
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/471

Abstract

Bacillus sphaericus H-5a5b is an entomoathogenic agent which showing high potency for vector control, highly specific to insect target, and do not produce any adverse environmental impact. Such agent would be promising for vector control in Indonesia. The present studies is aimed to observing the toxicity status of Bacillus sphaericus H-5a5b (VCRC B42) against Culex quiquefascitus larvae. The larvae of Culex quiquefascitus were reared under the laboratory conditions. The powder of VCRC B42 were prepared by Vector Control Research Centre (VCRC), India. Bioassays on VCRC B42 agains larvae of Culex quiquefascitus were also carried out under the laboratory conditions. The value of LD50 and LT50 for VCRC B42 were decided using probit analysis. The toxicity status was compared with standart of VCRC India. The result of the bioassays showed that the VCRC B42 was moderately toxic agains larvae of the Culex quiquefascitus. The value of LD50 for VCRC B42 was 0.058 mg/l and LT50 about 27 hours.
PERSISTENSI TOKSISITAS BIOINSEKTISIDA SPHEREFIXTM PADA BEBERAPA TIPE TEMPAT PERINDUKAN NYAMUK Aedesaegypti L. Salamun Salamun; S A Husein; Rosmanida Rosmanida; Dwi Winarni; Tri Nurhariyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 1 (2000): December 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/480

Abstract

SpherefixTM bioinsecticide is microbial agent of Bacillus sphaericus H-5a5b (VCRC B24) showing high potency for vector control. The bioinsecticide specific to target insect, and do not produce any adverse environmental impact, so the bioinsecticide would be very promising agent for vector control, especially vector of dengue haemorrhagic fever in Indonesia. The present studies aimed at observing the toxicity persistence of SpherefixTM on the larvae Aedesaegypti L. in some types of water container, and recycling potencies in the breeding places of A.aegypti. Two steps of the studies were carried out under laboratory conditions. Firststeps were reared mosquitos in the laboratory to supply larvae of Aedes aegypti. Second step testing of SpherefixTM toxicity persistence in the cemented, clay, and plastic containiers. The bioinsecticides were preparated by Vector Control Research Centre (VCRC), India. The toxicity persistence of SpherefixTM on the larvae of A. aegypti L. in some types of water container were decided by probit analysis. The toxicity persistence tests were carried out by time series observation on the day 1, 6, 12,,,, and 120. The result showed that difference toxicity persistence of the SpherefixTM in the breeding places of A. aegypti. The cemented container was found to have longest of toxicity persistence of the SpherefixTM followed by the plastic container, and the clay container ha the shortest duration toxicity persistence. The higher concentration of the boinsecticides, will result in higher toxicity persistence. The SpherefixTM showed recycling potency in the breeding places of A. aegypti. Percent motility of the larvae dropped sharply after day 50, and larvae mortality under 5% after day 78.
PENGARUH EKSTRAK AKAR GINGSENG JAWA DALAM BERBAGAI KADAR TERHADAP SINTESIS PROTEIN SERUM TIKUS Tri Nurhariyati; Dwi Winarni; Salamun Salamun; Y Sri Wulan Manuhara; Saikhu Ahmad Husen
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 1 (2000): December 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/482

Abstract

The aim of this research was identifying the stimulation serum protein synthesis following the treatment of Java Gingseng (Talinum paniculatum Gaertn) root extract in varying concentration. This research used 12 male rats of 8-9 weeks, divided into 4 groups. First group as control, second group to five group were administratered with Java gingseng root extract equal with 3.5, 10.5, and 21 mg dry roots / 100g BW / day per oral for 14 days. Blood serum was collected for electrophoresized at the end of treatment. Electrophoresis was conducted with 7% SDS-PAGE method. Gel stained in 0.25% Coomasie Brilliant Blue and it followed densitometry on band in gel to comparing those quantitatively. The result show the effect on the level of serum protein whose MW 29. Highest stimulation of protein synthesis (2.2 times than control) have been reached at treatment with extract that equal with 3,5 mg dry root/ 100 g BW/ day. Increasing Java gingseng root extract wasn’t followed by increasing of stimulation protein synthesis level.
Identifikasi Metabolit Sekunder dan Aktivitas Antimikrob Ekstrak Etil Asetat Dumortiera hirsuta Junairiah Junairiah; Muhimmatus Sa'diyah; Salamun Salamun
Sains dan Matematika Vol. 3 No. 2 (2015): April, Sains & Matematika
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Salah satu keanekaragaman flora di Indonesia adalah Dumortiera hirsuta. Tumbuhan ini berpotensi sebagai bahan antibakteri dan antifungi. Penelitian ini bertujuan untuk mengidentifi kasi dan menguji aktivitas antimikrob metabolit sekunder ekstrak etil asetat D. hirsuta. Metabolit sekunder diidentifi kasi dengan skrining fitokimia. Aktivitas antimikrob dilakukan dengan uji difusi dan uji dilusi terhadap Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Candida albicans ATCC 10231. Penelitian terdiri atas 24 perlakuan dan masing-masing terdiri atas tiga ulangan. Data yang diperoleh berupa diameter zona hambat, minimal inhibitary concentration (MIC), minimal bacterisidal/fungisidal concentration (MBC/MFC). Data dianalisis secara deskriptif. Berdasarkan hasil identifi kasi, diketahui bahwa ekstrak etil asetat D hirsuta mengandung flavonoid, alkaloid, steroid dan dapat menghambat mikrob patogen. One of the diversity of flora in Indonesia is Dumortiera hirsuta. This plant has the potential as an antibacterial and antifungal. This study aimed to identify and test the antimicrobial activity of secondary metabolites ethyl acetate extract of D. hirsuta. Secondary metabolites were identified by phytochemical screening. Antimicrobial activity performed by diffusion test and dilution test against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25 922 and Candida albicans ATCC 10231. This research consisted of 24 treatments and each consists of three replications. The obtained data were the diameter of inhibition zone, minimal inhibitory concentration (MIC), minimal bactericidal/fungicidal concentration (MBC/MFC). Data were analyzed descriptively. The identifi cation result showed that the ethylacetate extract of D hirsuta contained flavonoids, alkaloids, steroid, and can inhibit pathogen microbes.
INDIGENOUS BACILLUS SPECIES ISOLATED FROM AEDES AEGYPTI LARVAE: ISOLATION, LARVICIDAL TOXICITY SCREENING, PHENOTYPIC CHARACTERIZATION, AND MOLECULAR IDENTIFICATION Salamun; Rizky Danang Susetyo; Hakimatul Husniyah; Almando Geraldi; Ni’matuzahroh; Fatimah; Farah Aisyah Nafidiastri; Nabilatun Nisa’; Muhammad Fath Alhaqqi Sanis Salamy
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1938

Abstract

Vector-borne diseases transmitted by mosquitoes are considered a significant public health problem worldwide. Aedes aegypti is one of the mosquito species responsible for transmitting these diseases. One environmentally friendly method of vector control is the use of microbial agents such as Bacillus species. This study aimed to explore investigate indigenous entomopathogenic bacteria of Bacillus species isolated from A. aegypti larvae. Larvae samples were collected from breeding sites of A. aegypti. All isolates underwent screening and affirmation confirmation tests to assess their larvicidal toxicity against A. aegypti larvae. Phenotypic characterizations and molecular identifications were conducted to determine the species of the Bacillus isolates based on similarity index and percent identity (%ID). Phylogenetic trees were used to compare the isolates with other Bacillus species. The results revealed 120 isolates of Bacillus species from A. aegypti larvae samples. Among them, three isolates (LS3.3, LS9.1, and LSD4.2) exhibited the highest larvicidal toxicity in the confirmation test, resulting in larval mortality rates of 100%, 96.7%, and 100%, respectively, after 48 hours of exposure. Molecular identifications, showed that LSD4.2 had a 99.16% ID with Bacillus velezensis, LS3.3 had a 98.22% ID with Bacillus mojavensis, and LS9.1 had a 99.93% ID with Bacillus subtilis. These three bacteria from the Bacillus genus have been reported to offer significant benefits to humans.
Co-Authors Agus Supriyanto Almando Geraldi Dwi Winarni Farah Aisyah Nafidiastri Fatimah Fatimah Fatimah Hakimatul Husniyah Muhammad Fath Alhaqqi Sanis Salamy Muhimmatus Sa'diyah Nabilatun Nisa’ Nima’tuzahroh Nima’tuzahroh Ni’matuzahroh Ni’matuzzahroh R.A. Samsumaharto Rizky Danang Susetyo Rosmanida Rosmanida S A Husein Saikhu Ahmad Husen TATI NURHAYATI Tini Surtiningsih Tri Nurhariyati Y Sri Wulan Manuhara