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All Journal Jurnal Sain Veteriner METAMORFOSA Journal of Biological Sciences ASPIRATOR BIOTROPIC The Journal of Tropical Biology Jurnal Biologi Tropis Jurnal Entomologi Indonesia Jurnal Abdi Insani BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) BioEksakta : Jurnal Ilmiah Biologi Unsoed

Trisnowati Budi Ambarningrum

Fakultas Biologi, Universitas Jenderal Soedirman

Author-ID : 2895581

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Public Health Veterinary

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A Life Table of Spodoptera litura Fabr. with Different Artificial Diets Sri Lestari; Trisnowati Budi Ambarningrum; Hery Pratiknyo
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Sustainable insect supply is a routine activity in research of pest control, experimental insecticide bioassay, entomophatogen, parasitoid, and nature of competitor. Armyworm (Spodoptera litura Fabr.) is one of the alternative test insect. A mass rearing method is required to provide high demands of S. litura. A life table of insect is regularly used to study several biological parameters including the net reproduction rate of population (Ro), the mean length of life period in one generation (T), reproduction potential value of one generation from a population (rm), and the potential of a population to multiply in one generation (ë). The aim of this study were to asses the development time and reproduction potential of S. litura which different of artificial diets. Results showed that S. litura on artificial diet in recipe of Singh and Moore is T = 29,90, R = 2692,33, r = 0,26, ë = 1,29, o m whereas artificial diet in recipe of Waldbauer et al. is 32,29, R = 891,99, r = 0,21, ë = 1,23. o m

IPerforma Larva Lalat Tentara Hitam (Hermetia illucens) sebagai Biokonversi Limbah Industri Pengolahan Carica Dieng (Vasconcellea pubescens) di Wonosobo Irma Fatmanintyas; Trisnowati Budi Ambarningrum; Atang Atang; Trisno Haryanto; Eko Setiyono
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p13

Limbah berupa kulit dan biji yang berasal dari industri pengolahan carica Dieng (Vasconcellea pubescens) dapat menjadi permasalahan lingkungan apabila tidak tertangani dengan baik . Larva BSF (Hermetia illucens) sebagai agen biokonversi diharapkan mampu menjadi solusi untuk permasalahan tersebut. Penelitian dilakukan secara eksperimental dengan menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan dan 4 ulangan meliputi perlakuan kontrol, P1 = 100% kulit, P2 = 75% kulit + 25% biji, P3 = 50% kulit + 50% biji, P4 = 25% kulit + 75% biji, dan P5 = 100% biji. Waktu pengamatan penelitian dilakukan selama 21 hari. Parameter utama yang diamati adalah nilai konsumsi pakan dan indeks pengurangan limbah (WRI), sedangkan parameter pendukung berupa biomassa larva, diameter kapsul kepala dan tingkat kelulusan hidup (survival rate). Data yang diperoleh dianalisis menggunakan ANOVA dengan tingkat kesalahan 5%, dan apabila perlakuan berpengaruh nyata, dilanjutkan dengan uji DMRT dengan tingkat kesalahan 5%. Hasil penelitian dengan jenis pakan yang bervariasi menunjukkan nilai konsumsi pakan tercerna oleh larva BSF berkisar antara 60,42% - 81,26%. Sedangkan untuk nilai indeks pengurangan limbah (WRI) berkisar antara 2,82% - 3,73%. Nilai reduksi limbah lebih dari 50% menunjukkan adanya efektivitas larva BSF dalam mendegradasi limbah organik. Dengan demikian penggunaan jenis pakan berupa pakan penggunaan larva BSF efektif dalam mereduksi limbah pengolahan carica Dieng (V. pubescens).

Eliminasi Endosimbion Wolbachia sp. pada Nyamuk Aedes albopictus dengan Antibiotik Tetrasiklin Endang Srimurni Kusmintarsih; Darsono Darsono; Edy Riwidiharso; Rokhmani Rokhmani; Trisnowati Budi Ambarningrum; Endang Ariyani S
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 17 Nomor 2 Desember 2021
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/blb.v17i2.4249

Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus which is transmitted through the bite of the Aedes mosquito. Aedes aegypti, which is known as the primary vector of dengue virus, is naturally not infected by Wolbachia sp. endosymbiont, while Ae. albopictus which is a secondary vector naturally infected with Wolbachia sp. The Wolbachia sp. known to inhibit the transmission of Dengue virus, to study the mechanism, it is necessary to eliminate Wolbachia sp. from Ae. albopictus, then infects the Ae. albopictus with the Dengue virus. The aim of the study was to determine the ability of tetracycline antibiotics to eliminate Wolbachia sp. from the Ae. albopictus mosquito. Ae. albopictus eggs was obtained in the Ciamis area by survey method using ovitrap which was installed outside the house. The Ae. albopictus eggs are then incubated in the laboratory and reared until they become adult mosquitoes. Mosquitoes were treated with sugar feeding which had been given tetracycline 0.25mg/ml every two days alternated with blood feed. Detection of the presence of Wolbachia sp. on mosquitoes carried out in first to third generations by the polymerase chain reaction (PCR) method using Wsp-specific primers. The results showed that the first to third generation mosquitoes were still infected with Wolbachia sp. This shows that the dose of tetracycline antibiotics used has not been able to eliminate Wolbachia sp. from the Ae. albopictus mosquito.

Status dan perkembangan resistensi Aedes aegypti (Linnaeus) (Diptera: Culicidae) strain Bandung, Bogor, Makassar, Palu, dan VCRU terhadap insektisida permetrin dengan seleksi lima generasi Yerslin Mantolu; Kustiati Kustiati; Trisnowati Budi Ambarningrum; Sri Yusmalinar; Intan Ahmad
Jurnal Entomologi Indonesia Vol 13 No 1 (2016): Maret
Publisher : Perhimpunan Entomologi Indonesia

Aedes aegypti (Linnaeus) (Diptera: Culicidae) is the main vector of dengue fever. In Indonesia, insecticides, especially pyrethroids, such as permethrin, have been effectively used to control Ae. aegypti. Notwithstanding that permethrin has been used since 1980s, information regarding the status and development of resistance of Ae. aegypti to permethrin is still limited and need further update. This study was conducted using the WHO standard test method. The aims was to determine the resistance status, and changes in resistance level of four field strains (Palu, Makassar, Bandung, Bogor) and susceptible strain (VCRU) of Ae. aegypti after the selection with permethrin for five generations. The results showed that resistance status of all field strains to permethrin were considered as high. The value of RR50 ranged between 10.5 to 25.7 fold. Bandung strain had the highest value of RR50 (22.5 fold), while Makassar strain had the lowest value of RR50 (10.5 fold). The fifth generation (F5) of five selected Ae. aegypti strains had the level of resistance 5 to 18 times higher than their parental. Knowledge of resistance status in a given area accompanied with the understanding about the development of resistance can be used to design a better vector management.

Deteksi Molekuler Virus Chikungunya pada Nyamuk Aedes aegypti Menggunakan Metode Two Step RT PCR Alvira Rifdah Sativa; Endang Srimurni Kusmintarsih; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

The Chikungunya fever is a disease caused by an Alphavirus from the family Togaviridae with the symptoms of a patient's posture that is bent over by severe joint pain (arthalgia). Chikungunya disease can be transmitted to humans through an Aedes aegypti as a vector. The outbreak of Chikungunya in Indonesia was first reported in 1973 in Samarinda and then spread to various other regions. Surveillance data show almost every year of the outbreak occurred in various regions in Indonesia. In 2013 there was the outbreak of Chikungunya in North Purwokerto, especially in Bancarkembar and Grendeng.Until now, the medicine or vaccine has not been found to prevent Chikungunya disease. The purpose of this research is to know the infection of virus Chikungunya on Ae. aegypti. This research was conducted by surveying methods with sampling techniques using purposive sampling The observed parameter is th positivity of virus Chikungunya. Analysis data by observing the appearance of DNA band on UV Transilluminator. The results that the amplikon cDNA CHIKV is not detected by the two step RT-PCR method. Key Words : Aedes aegypti, Chikungunya, DNA, Vector

Perilaku Memilih Umpan Dengan Fagostimulan Yang Berbeda Pada Kecoak Jerman Blattella germanica L. (Dictyoptera: Blattellidae) Rizky Arjunnajat Aliefia; Trisnowati Budi Ambarningrum; Edi Basuki
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2007

German cockroach (Blattella germanica L.) is one of the important residential pest insects, because of its role as a vector of disease. To reduce its population, it can be used with bait techniques. However, there was a rejection of glucose against German cockroaches against phagostimulant-based commercial bait. Therefore it is necessary to review the phagostimulant as a component of the bait to minimize failure to control German cockroaches based on bait using German cockroaches strain VCRU (Vector Control Research Unit). The first step to formulating the bait is to find the phagostimulant most sought by German cockroaches. The ingredients used as fagostimlan are sugar, durian, erythritol, and banana. The purpose of this study was to determine the behavior of choosing German cockroaches against bait with different phagostimulants and the peak of feeding activity in German cockroaches. This study used an experimental method with a Completely Randomized Design (CRD) consisting of 4 treatments giving phagostimulant and repeated 4 times divided into four test arenas. The treatment consisted of bait which each contained sugar, durian, erythritol, and banana as phagostimulants. Observation of behavior using the method of behavior sampling is recorded in continuous recording for 24 hours using a Closed Circuit Television (CCTV) camera. The parameters measured were latency, frequency, duration, and peak feeding activity of German cockroaches. The data were analyzed with ANOVA at p <0.05. The results showed that the behavior of selecting cockroaches from the VCRU strain on the bait was not significantly different (p<0.05) and was attracted to all feeds provided, but gel bait with sugar phagostimulant was the preferred bait by looking at the three parameters of latency, frequency, and duration. The results of the average latency in the VCRU strain to the durian gel for 17 minutes, the average frequency of most visits to the sugar gel in the VCRU strain by 10 times, the longest average duration of the VCRU strain gel for 1 minute 46 seconds and peak eating activity VCRU strains occur between 17:00 - 20:00.

Deteksi Molekuler Virus Dengue dan Chikungunya pada Nyamuk Aedes spp. di Kecamatan Cilongok Dwi Iva Fitriana; Endang Srimurni Kusmintarsih; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Kecamatan Cilongok merupakan salah kecamatan endemis DBD dan pernah mengalami KLB chikungunya. Deteksi virus pada nyamuk sebelum menginfeksi manusia penting sebagai peringatan dini dalam upaya pencegahan wabah di daerah endemis. Tujuan penelitian ini adalah mengetahui infeksi virus Dengue dan Chikungunya pada nyamuk Aedes spp. asal kecamatan Cilongok. Penelitian ini dilakukan di empat desa di Kecamatan Cilongok yang meliputi Desa Cilongok, Pernasidi, Kalisari, dan Jatisaba. Desain penelitian ini adalah studi purposive random sampling . Deteksi virus Dengue dan Chikungunya pada nyamuk dilakukan menggunakan teknik Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR). Hasil positif virus dianalisis secara deskriptif untuk menggambarkan potensi transmisi virus. Hasil penelitian menunjukkan bahwa nyamuk Aedes spp. yang tertangkap tidak mengandung virus Dengue dan Chikungunya. Kata kunci: Aedes spp., chikungunya virus, dengue virus, teknik RT-PCR

Kelimpahan Tungau Predator pada Tanaman Melati Gambir (Jasminum officinale) di Desa Cipawon, Bukateja, Purbalingga, Jawa Tengah Ika Rakhmayani; Bambang Heru Budianto; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3927

The Gambier Jasmine plant (Jasminum officinale) is located in Bukateja District, Cipawon Village, Purbalingga, Central Java is a commodity with high economic value, its use a tea fragrance, cosmetics, perfumes dan others. One of the cause of decreased productivity of Gambier Jasmine is ude to the presence of pets, namely mites. The pest mite population is controlled by predatory mites. Therefore, this study aimed to determne the types of predatory mites and determine the abundance of predatory mites in Gambier Jasmine (J. officinale) in Cipawon Village, Purbalingga, Central Java. This research used survey method with purposive sampling technique. There are nine sampling points, eight pointa on the outer edge and one point in the middle of plantation. Each sampling is represented by three trees. The sample in the form of Gambier Jasmine Leaves came form the lowest ten leaf stalks in one tree at each sampling point. The number of leaf samples obtained was 270 leaf samples. Each sampling point was taken three times with one week intervals. The variable observed include the number of individuals and the number of species of predatory mites on the Gambier Jasmine (J. officinale) and the parameters calculated were leaf area, leaf sitting angle, length and density of trichomes, temperature, humidity, asa well as rainfall. The data obtained were analyzed by analysis of variance (ANOVA) with an error rate of 20%. The result of the identification of predatory mites on the Gambier Jasmine plant were obtained by two species, namely Amblyseius largoensis and Phytoseius amba. A. largoensis as many as 76 individuals with an abundance percentage of 61,8% and P. amba as many as 47 individuals with an abundance percentage of 38,2%. The abundance of predatory mites A. largoensis and P. amba is classified as moderate because predators are easy to find, every three leaves there is one predator. Key words: abundance., Cipawon., Gambier Jasmine., predatory mites.

Efektivitas Zat Aktif Fipronil pada Berbagai Substrat Fagostimulan untuk Pengendalian Kecoak Jerman (Blattella germanica L.) Riska Isna Maula; Hery Pratiknyo; Untung Susilo; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

German cockroach (Blattella germanica L.), is a residential insect pest and vector of various diseases. To control its population, alternative chemical control models with Fipronil 0.03% are now being developed. Fipronil has been proven to be effective and has no resistance. To increase the effectiveness of the bait in controlling German cockroaches, a fipronil 0.03% combination needs to be developed with a phagostimulant bait of bananas, durian, erythisol sugar, and sugar formulation, so this study needs to be done. This study used an experimental method with a randomized block design (RBD), five treatments such as A: bait with a combination of banana phagostimulant and 0.03% fipronil, B: bait with a combination of durian phagostimulant and 0.03% fipronil, C: bait with a combination erythisol phagostimulant and 0.03% fipronil, D: bait with a combination of sugar phagostimulant and 0.03% fipronil, K: bait with a combination of matrix and fipronil 0.03% (control positive) was used for the interest test, and bait with a matrix without fipronil 0.03% (control negative) was used for mortality testing. The observations the average results for durian, banana, sugar formulated, erytrisol sugar and controls, respectively 20%, 20%, 21.6%, 12.4%, and 22.8%, while the results bait consumed with durian stimulation are 0.29g, banana 0.31g, sugar formulation 0.28g, erytrisol sugar 0.26g and control 0.24g. However, based on statistical tests, the results of the interest test with the feed consumption test between treatments showed no significant difference (p>0.05). The test results in mortality of cockroaches Germany's feed with phagostimulan durian, banana, formulations sugar, sugar erytrisol and control consecutively 98% 100 %, 98%, 98%, and 20%, based on the statistical test there is a significant difference (p<0.05), where the difference based on the Duncan test is their treatment compared to control.

Ketertarikan dan Kesukaan Kecoak Jerman, Blatella germanica L. (Dictyoptera: Blattellidae) Terhadap Fagostimulan Berbeda Femilia Hajar Ilhami; Trisnowati Budi Ambarningrum; Hery Pratiknyo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

German cockroaches (Blatella germanica) L. are includes in settlement bugs that are hard to terminate. One alternative to control German cockroaches that are using bait. To make bait formulation, the first thing is look for the type of phagostimulant that can be interesting and attractive to German cockroaches. The purpose of this research is to study the type of phagostimulant that has the ability to attract and liked by German cockroaches. Phagostimulant types used are bananas, durian, sugar formulations, and erythrisol. Data from observations of interest in test interest were analyzed using the t test and calculation of the attraction index, while the data for German roaches favored were analyzed using ANOVA and index feeding calculations. The results obtained were that German cockroaches were attracted to bananas, durian, sugar formulations, and erythrisol. ANOVA analysis results obtained sig 0.029 (p <0.05). The results of the calculation of the attractiveness index and feeding index obtained German cockroaches are preferred by bananas compared to other phagostimulants. The conclusion of this study is the type of phagostimulant that can attract cockroaches Germany is banana, durian, sugar formulation, and erythrisol, while the type of phagostimulant most preferred by German cockroaches is banana.

Co-Authors -, Mutala'liah Aliefia, Rizky Arjunnajat Alvira Rifdah Sativa Annisa, Dyah Retno Arif Rahman Hikam Atang Atang Atang, Atang Bambang Heru Budianto Darsono Darsono Dwi Iva Fitriana Dwi Sarwani Sri Rejeki Dwiana Muflihah Yulianti Dyah Fitri Kusharyati Dyah Retno Annisa Edi Basuki Edi Basuki Edi Basuki Edy Riwidiharso Emyliana Listiowati, Emyliana Endang Srimurni Kusmintarsih ENDANG SRIMURNI KUSMINTARSIH ENDANG SRIMURNI KUSMINTARSIH ENDANG SRIMURNI KUSMINTARSIH Endang Srimurni Kusmintarsih Fathimah Nurfithri Hashifah Femilia Hajar Ilhami Fitriana, Dwi Iva Haryanto, Trisno Hashifah, Fathimah Nurfitri Hery Pratiknyo Hery Pratiknyo Hery Pratiknyo Hilmiyah Al'alawiyah Ika Rakhmayani Ilhami, Femilia Hajar Intan Ahmad Irma Fatmanintyas Irwan Susanto Kustiati Kustiati Maula, Riska Isna Niken Istikhari Muslihah Nirwani, Shintya Nitra Nuranisah, Annanda Pamudji, Ganjar Rakhmayani, Ika Rifai, Lutfin Agri Riska Isna Maula Rizky Arjunnajat Aliefia Rokhmani Rokhmani Sativa, Alvira Rifdah Setiyono, Eko Setyanto, Refius Pradipta Setyawati, Endang Ariyani Sri Lestari Sri Lestari Sri Yusmalinar Taruna Dwi Satwika Trisno Haryanto Untung Susilo Wilda Khafida Yerslin Mantolu

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