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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner Jurnal Kedokteran Brawijaya Jurnal Ilmu Ternak Veteriner Indonesian Journal of Biotechnology Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) ARSHI Veterinary Letters Veterinary Biomedical and Clinical Journal Jurnal Nasional Teknologi Terapan IGKOJEI: Jurnal Pengabdian Masyarakat Jurnal Veteriner Nusantara
Ida Tjahajati
Bagian Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan UGM
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Reactive Oxygen Intermediate (ROI) in Dog Macrophage Infected with Mycobacterium tuberculosis Tjahajati, Ida
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.109 KB)

Abstract

experiment used 24 healthy dogs, aged between 1 and 2 years, both male and female which were divided into twodifferent groups consisting of 12 dogs each. The first group was the treatment group, that is they were infected with Mtuberculosis and the second one was the control group. The activity of macrophages ROI secretion were measured at1st, 2nd, 12th, and 24th after infection using nitroblue tetrazolium (NBT) reduction assay. Three cats were used to measure themacrophage activity in each period, using triplicate sample for each cat. The results of the experiment showed thatROI secretion increased in infected group compared with the control group, and this activity reached to the plateaulevel at 2 weeks after infection. Although these enhanced activities were gradually diminished thereafter, higherlevels of these activities were consistently observed until the end of experiment compared with control group. Theresults of the experiment indicated that ROI played an important role to against M.tuberculosis infection in dogs.Keyword: macrophage, ROI, M.tuberculosis, dogs
VAKSINASI BCG MENINGKATKAN AKTIVITAS FAGOSITOSIS DAN SEKRESI REACTIVE OXYGEN INTERMEDIATE (ROI) PADA MAKROFAG PERITONEUM KUCING YANG DIINFEKSI DENGAN Mycobacterium tuberculosis Tjahajati, Ida
Jurnal Kedokteran Brawijaya Vol 21, No 2 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (985.824 KB) | DOI: 10.21776/ub.jkb.2005.021.02.8

Abstract

ABSTRACT The aim of this experiment is to study the effect of BCG vaccination on phagocyte activity and ROI secretion in cat peritoneum macrophages which infected with M.tuberculosis. The experiment used twenty four healthy cats. The animals were divided in 2 groups, 12 cats in each group. Group I were vaccinated with BCG, group II were control group which unvaccinated. BCG vaccination was done twice in two weeks interval. Two days after vaccination each cat was infected by 105 cfu M.tuberculosis intraperitoneally. The activity of macrophages were measured  at 1st, 2nd, 12th, and 24th, after infection using in vitro latex bead phagocyte and NBT reduction assay. Three cats were used to measure the macrophage activity in each period, using triplicate sample for each cat. The results of the experiment showed that the phagocyte activity and ROI secretion increased significantly in vaccination group (P<0.01) compared with the control group, and these activities reached to the plateau level at 2 weeks after infection. Although these enhanced activities were gradually diminished thereafter, higher levels of these activities were consistently observed until the end of experiment compared with control group. BCG vaccination increased the cellular immunity especially phagocyte and ROI secretion activities of peritoneual macrophages in cat infected with M.tuberculosis Key words: BCG, phagocyte, ROI, M.tuberculosis, cat
PENGEMBANGAN DIAGNOSIS TUBERKULOSIS PADA HEWAN KESAYANGAN ANJING MENGGUNAKAN ANTIGEN SPESIFIK Mycobacterium tuberculosisESAT-6 DAN CFP-10 Tjahajati, Ida; Asmara, Widya; Soebono, Hardyanto
Jurnal Kedokteran Brawijaya Vol 23, No 2 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1627.133 KB) | DOI: 10.21776/ub.jkb.2007.023.02.1

Abstract

Early diagnosis is one of the important methods to  control tuberculosis because this disease is zoonotic which easily spread through the air. Early detection of tuberculosis in dog is also very important since dog as petanimal have a potency to transfer the disease to human or other animals. The discovery of two specific M.tuberculosisantigens, ESAT06 and CFP-10, provide the opportunity to developa specific diagnostic kit for tuberculosis by using ELISA based on the secretion of IFN-γ. The development of a tuberculosis diagnostic kit based on this molecular biology and immunological method would provide a good alternative method to detect tuberculosis specially, accurately as early as possible. The result of this experiment would provide contribution for the development of health science and technology, especially in the eradication of tuberculosis. Keywords: diagnosis, tuberculosis, ESAT-6, CFP-10, interferon-gamma.
VAKSINASI BCG MENINGKATKAN AKTIVITAS FAGOSITOSIS DAN SEKRESI REACTIVE OXYGEN INTERMEDIATE (ROI) PADA MAKROFAG PERITONEUM KUCING YANG DIINFEKSI DENGAN Mycobacterium tuberculosis Tjahajati, Ida
Jurnal Kedokteran Brawijaya Vol 21, No 2 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (971.59 KB) | DOI: 10.21776/ub.jkb.2005.021.02.3

Abstract

ABSTRACT The aim of this experiment was to study the effect of BCG vaccination on phagocyte activity and ROI secretion in cat peritoneum macrophages which infected with M.tuberculosis. The experiment used twenty four healthy cats. The animals were divided in 2 groups, 12 cats in each group. Group I were vaccinated with BCG, group II were control group which unvaccinated. BCG vaccination was done twice in two weeks interval. Two days after vaccination each cat was infected by 105 cfu M.tuberculosis intraperitoneally. The activity of macrophages were measured  at 1st, 2nd, 12th, and 24th, after infection using in vitro latex bead phagocyte and NBT reduction assay. Three cats were used to measure the macrophage activity in each period, using triplicate sample for each cat. The results of the experiment showed that the phagocyte activity and ROI secretion increased significantly in vaccination group (P<0.01) compared with the control group, and these activities reached to the plateau level at 2 weeks after infection. Although these enhanced activities were gradually diminished thereafter, higher levels of these activities were consistently observed until the end of experiment compared with control group. BCG vaccination increased the cellular immunity especially phagocyte and ROI secretion activities of peritoneual macrophages in cat infected with M.tuberculosis Key words: BCG, phagocyte, ROI, M.tuberculosis, cat
Sosisalisasi Kesehatan Hewan Kepada Masyarakat Secara Daring Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Raharjo, Slamet; Nururrozi, Alfarisa; Wuryastuty, Hastari; Widiyono, Irkham; Purnamaningsih, Hary; Mulyani, Guntari Titik; Tjahajati, Ida; Hartati, Sri; Yuriadi, Yuriadi
IGKOJEI: Jurnal Pengabdian Masyarakat Vol 2 No 2 (2021): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v2i2.223

Abstract

ABSTRACT Various animal diseases are not always known by animal owners, so they must always be socialized even in the condition of the COVID-19 outbreak. This community service aims to provide animal health information to the community by online. This activity was carried out from in November 2020 using the Cisco Webex platform. The results showed that as many as 269 participants have joined in the socialization, consisting of 180 from Yogyakarta and 89 from outside Yogyakarta. Concluded that online socialization of animal health is successfully carried out and received a very good response from the community. Keywords: Animal health; Community service; Veterinary clinic ABSTRAK Berbagai macam penyakit hewan tidak selalu diketahui oleh pemilik hewan, sehingga harus selalu disosialisasikan walapun dalam kondisi wabah COVID-19. Pengabdian kepada masyarakat (PKM) ini bertujuan memberikan informasi kesehatan hewan kepada masyarkat secara daring. Pengabdian dilakukan pada bulan bulan Oktober sampai Nopember 2020 secara daring menggunakan platform Cisco Webex. Hasil PKM menunjukkan bahwa sebanyak 269 calon peserta telah mengikuti sosialisasi, yang terdiri dari 180 berasal dari Yogyakarta dan 89 dari luar Yogyakarta. Berdasar hasil kegiatan ini, disimpulkan bahwa sosialisasi tentang kesehatan hewan secara daring berhasil dilaksanakan dan mendapat respon sangat bagus dari masyarakat. Kata kunci: Kesehatan hewan; Klinik hewan; Pengabdian masyarakat.
SA-2 Representation of Various Systematic Disruptions within the Patients Handled at “Klinik Hewan Jogja” between January and June 2018 Ida Tjahajati; Adellyna Chrissandra; Anggi D Prayitno; Begum F R Aditya; E Rrarindah; L Rifiyanta; M A Candra; T A Widiastuti; S I Astuti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.398 KB)

Abstract

Various diseases or disruption could affect all of the patients handled at any clinic or hospital. The health problems would be varied, depending on the disease or systemic disruption that ailed the patient. This research aims to figure out the spread of said health problems in the patients handled at Klinik Hewan Jogja between January and June 2018.A numerical representation of afflictions that ails the patients in a clinic or a hospital during a period of time would provide the information needed to anticipate several diseases, facilitate easier treatment and follow-up actions for certain ailments, offer prevention methods, and lastly to yield data for clients' education. The data gathered is also vital to give a general picture the problems that frequent a certain area, thus imparting important information to all other clinics or hospitals in the said region.
SA-6 Foreign Objects in the Form of Nails Found Inside the Gastrim of a Mixed Breed Dog Patient Handled at Klinik Hewan Jogja Ida Tjahajati; Adellyna Chrissandra; Anggi D Prayitno; Begum F R Aditya; E Rrarindah; L Rifiyanta; M A Candra; Sri Indah Astuti; T A Widiastuti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.506 KB)

Abstract

Various diseases could affect dogs, including disruptions of the gastrium caused by the presence of foreign objects. The dogs’ habit of consuming miscellaneous objects around them was the common cause for the said objects, which should not exist within their body, to be swallowed and enter the stomach. This kind of case is often found among the growing puppies of large breed dogs [1]. This report aims to reveal the case of foreign objects finding within the gastrium of a dog patient handled at Klinik Hewan Jogja. In this particular case, we found foreign objects in the form of many nails and an assortment of metal wire pieces inside the gastrium of mixed breed dog Messi.
SA-12 The Number of Dog Patients Infected with Companion Vector-Borne Diseases (CVBD) Treated at Klinik Hewan Jogja in 2017 Ida Tjahajati; Adellyna Chrissandra; Anggi D Prayitno; Begum F R Aditya; E Rrarindah; L Rifiyanta; M A Candra; Sri Indah Astuti; Tri Ayu Widiastuti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (677.129 KB)

Abstract

Companion Vector Borne Diseases (CVBD) are diseases transmitted by vectors which can cause fatal outcome for dogs [1]. CVBD frequently attack dogs and are easily transmitted from one dog to other dogs through vectors’, such as ticks, mosquitoes, or sand flies, bite [2,3]. Some of the most harmful CVBD that infect dogs via ticks’ bite are anaplasmosis, babesiosis, and ehrlichiosis [4]. These diseases cause decreased appetite, lethargy, fever, anemia, jaundice, emaciation, and finally death if not treated properly [5,6].Blood parasites’ infections such as anaplasmosis, babesiosis, and ehrlichiosis are often found in the field. However, due to the limitations of diagnosis confirmation, they often go unnoticed. Despite the considerable number of cases in dogs, the exact incidence rate is not yet known. There is an global call to eradicate CVBD owing to the facts that they are dangerous and may cause economic losses, with the additional risk of being zoonotic [1,3].Taking the importance of anaplasmosis, babesiosis, and ehrlichiosis in the veterinary science into account, seeing the scant amount of data regarding dog patients followed with the lack of attention on said diseases, it is then deemed necessary to properly diagnose those diseases in dogs brought into the clinic, particularly at Klinik Hewan Jogja. This research aims to identify the number of dog patients treated in Klinik Hewan Jogja infected with those particular sicknesses (CVBD).The result of this study will provide a summary of CVBD treated at Klinik Hewan Jogja, which in turn, will raise awareness of the diseases and the significance of working together to tackle the issue. Furthermore, the result will also yield information for clients’ education to help them prevent the diseases.
Effect of Mix Culture Bacteria and Fungi in Fermented Peanut Hulls-Based Feed Supplement on Physical Quality and In Vitro Rumen Fermentation Parameters DELA RIA NESTI; Ahmad Baidlowi; Achmad Fauzi; Ida Tjahajati
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 1 (2020): MARCH 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i1.2079

Abstract

The purpose of this research was to determine the effect of the combination of mix culture bacteria (EM4®:E) and fungi (Trichoderma viridae:TV) on physical quality and in vitro rumen fermentation parameters of peanut hull-based feed supplements. Basal feed was divided into four treatments, which were: P0 (BF); P1 (E:25%+TV:75%); P2 (E:50%+TV:50%),; and P3 (E:75%+TV:25%); and each treatment had three replications. Feeds were fermented facultative anaerobically for nine days. The observed parameters were physical qualities (color, odor, fungi appearance and pH), and in vitro rumen fermentation parameters (rumen pH, microbial protein content, and total volatile fatty acid content). Data were analyzed using one way ANOVA design, and the significance of differences were tested using Kruskall-Wallis test for the physical qualities data and Duncan’s New Multiple Range Test (DMRT) test for in vitro rumen fermentation parameters. Results showed that the combination of E and TV at different level did not affect odor and the fungi appearance parameters (P≥0.05), however, it significantly affected P≤0.05) color change from blackish (1.64) in P0 to brownish in P1, P2 and P3 of 2.44; 2.69; and 2.80, respectively. The pH also decreased significantly (P≤0.05) by 10.67%.  Treatment also did not affect the rumen pH, microbial protein content, and total volatile fatty acid content (P≥0.05). It is concluded that the combination of the EM4® 25%: Trichoderma viridae 75% on peanut-hull based feed supplement fermentation gives the best result on color and pH fermented feed product without affecting the rumen fermentation process.
Reactive Oxygen Intermediate (ROI) in Dog Macrophage Infected with Mycobacterium tuberculosis Ida Tjahajati
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.109 KB) | DOI: 10.22146/ijbiotech.7568

Abstract

experiment used 24 healthy dogs, aged between 1 and 2 years, both male and female which were divided into twodifferent groups consisting of 12 dogs each. The first group was the treatment group, that is they were infected with Mtuberculosis and the second one was the control group. The activity of macrophages ROI secretion were measured at1st, 2nd, 12th, and 24th after infection using nitroblue tetrazolium (NBT) reduction assay. Three cats were used to measure themacrophage activity in each period, using triplicate sample for each cat. The results of the experiment showed thatROI secretion increased in infected group compared with the control group, and this activity reached to the plateaulevel at 2 weeks after infection. Although these enhanced activities were gradually diminished thereafter, higherlevels of these activities were consistently observed until the end of experiment compared with control group. Theresults of the experiment indicated that ROI played an important role to against M.tuberculosis infection in dogs.Keyword: macrophage, ROI, M.tuberculosis, dogs
Co-Authors Achmad Fauzi Adellyna Chrissandra Ahmad Baidlowi Ahmad Baidlowi Aidah Rahmanita Akbar Wicaksono Albert Jackson Yang Alfarisa Nururrozi Alfarisa Nururrozi, Alfarisa Alfariza Nururozi Angeline Ganapragasam Anggi D Prayitno Aris Haryanto Aris Haryanto Bagas Pria Prasetyo Baidlowi, Ahmad Bambang Hariono Begum F R Aditya Budiono Santoso Dionisius M. . Dwi Priyowidodo E Rrarindah Fa'iz Ihsanul Kamil Farhan Arhabi Prima Fathur Rohman Haryadi Febri Ariyanti Guntari Titik Mulayni Guntari Titik Mulyani Gustaf Eifel Silalahi Gustaf Eifel Silalahi Hardyanto Soebono Hardyanto Subono Harjono, Habib Prasetyo Hary Purnamaningsih Hary Purnamaningsih Hastari Wuryastuty Hastari Wuryastuty Hastari Wuryastuty Hastari Wuryastuty Herawati, Okti I Gusti Made Krisna Erawan Imron Rosyadi Indarjulianto , Soedarmanto Irkham Widiyono Kelviano Muqit L Rifiyanta Laksono Trisnantoro Lily Gunawan M A Candra Muh. Disna Faizal Nabilah Putroe Agung Nesti, Dela Ria Nobuyuki Harada Pamudya, Kanya Gabriella Sasri Pramono, Agung Budi Raden Wisnu Nurcahyo S I Astuti Sinusi, Muhammad Zulfadillah Slamet Rahardjo Slamet Raharjo Slamet Raharjo Slamet Raharjo Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Hartati Sri Hartati Sri Hartati Sri Indah Astuti Subronto Prodjoharjono Subronto Prodjoharjono Sukma, Salsabila Ayuningtyas T A Widiastuti Tri Ari Widiastuti Tri Ayu Widiastuti Wibisono, Cahyo Widagdo Sri Nugroho Widiasih , Dyah Ayu Widya Asmara Wijayanti , Agustina Dwi Wisnu Nurcahyo Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono, Yanuartono Yeo Suan Jiao Yuriadi . Yuriadi Yuriadi Yuriadi Yuriadi Yuriadi Yuriadi Yuriadi Yuriadi, Yuriadi Yustinus Oswin Primajuni Wuhan