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All Journal Buletin Peternakan Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology The Indonesian Biomedical Journal Livestock and Animal Research JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology) Jurnal Agripet

Tety Hartatik

Faculty Of Animal Science, Universitas Gadjah Mada

Author-ID : 276877

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Public Health Veterinary Other

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Morphological diversity and genetic differentiation of PO cattle in smallholder farmers Hartatik, Tety; ., Hartati; ., Sumadi; ., Subandriyo
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

PO cattle is one ofÂ the local cattle with high genetic diversity. The aim of this research was to study genetic diversity of PO cattle in smallholder farmerÂ based on morfology and molecular markers. This research was conducted at breeding center in East Java and Central Java include Tuban, Lamongan and Blora regency, since June until December 2008. Â PO bull used were of 18 months until 24 months of age and cow of 24 months until 36 months of age or at first calving as much as 30 head each location. The variables measured were body weight, body length, withers height, hip height, shoulder point width, chest girth, chest depth, canon bone circumference, head length and head width. Data were analyzed using descriptive statistic, average analysis between subpopulations, discriminant and canonical analysis and mahalanobis distance was done by SAS DISCRIM procedure. Whole blood were collected to get the data of polymorphism DNA microsatellite using technology of PCR and electrophoresis using 6 microsatellite loci. Variable measured were number and size of Â alel, alel frequency, heterozygosity,genetic distance and fixation index (F-stat). Data were analyzed using analysis of Microsate Toolkit, GENEPOPV4, MSA (Microsatellite Analyser) and Mega 4. The result showed that Blora and Tuban subpopulation have genetic diversity which is relative lowerÂ compared to that of Lamongan. The genetic distance which was close presented by Tuban and Blora subpopulation while Lamongan and Tuban subpopulation show far genetic distance. The result of canonical analyses showedÂ high correlation on shoulder point width, head width, body length, withers height and hip height variable so this five variable can be used as distinguishing variables among subpopulation. The result of molecular genetics analysis using microsatellite showed that highest frequency of alel presented by HEL9 locus at Tuban population so this loci have the high polymorfism. The genetic differentiation among subpopulation showed by differentiation FST value among six lociÂ indicating the increasing of inbreeding in Â the three subpopulations. Key words: Morphological Diversity, Genetic Differentiation, PO Cattle

The Application of Polymerase Chain Reaction â Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island Hartatik, Tety; Volkandari, Slamet Diah; ., Sumadi; ., Widodo
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.889 KB)

The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. Samples used for the experiments were blood of 43 cattle that consist of 15 cattle obtained from Madura Island, 23 cattle from Sapudi Island, and 5 Limousin-Madura (Limura) cattle. A fragment of 464 base pair of cytb gene was amplifi ed by forward primer L14735 and reverse primer H15149. The PCR product was digested with TaqIand HinfI restriction enzymes to identify genetic patterns. Data of PCR-RFLP showed two haplotypes, that were A and B, in cattle obtained from both Madura Island and Sapudi Island. The frequencies of haplotype A and B of cattle from Sapudi Island were 69.57% and 30.47%, respectively. More diverse frequencies were observed in cattle obtained from Madura Island, where haplotype A and B were 86.67% and 13.33%, respectively. In this experiment, Limura cattle had only haplotype A. As a conclusion, PCR-RFLP of the cytb gene had been able to determine a genetic diversity of Madura cattle.Key words: Genetic diversity, Madura cattle, haplotype.

Pendugaan Model Kurva Pertumbuhan Berat Badan Pada Sapi Aceh (Bos indicus) Widya Pintaka Bayu Putra; Sumadi Sumadi; Tety Hartatik; Hendra Saumar
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 4 No 1 (2018): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Growth traits represent mirroring of individual availability to present genetic potency and growth on parts of the body. Observation on growth rate was based on the changes in body weight (BW) by adding ages from birth (1 day of age) to maturity age (>1261 days) periods from 142 animals (98 males and 44 females). Information of the ages was obtained based on herd records from 2010 - 2013. Two growth curve models consisted of Richard and Weibull models were used in this study based on CurveExpert 1.4 computer program. Research results showed that both models have a similar coefficient of determination (R2 = 0.99 ), but the lowest standard error (SE) value reached in Richard model (2.45). The optimum growth rate was reached until the age of 18 months (Richard) and 20 months (Weibull) with an optimum weight of about 135 kg for both models. As a result, selection based on weight at 18 months (550 days) of age was important for selecting the best bull and heifer.

Morphological diversity and genetic differentiation of PO cattle in smallholder farmers Hartati .; Sumadi .; Subandriyo .; Tety Hartatik
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

PO cattle is one of the local cattle with high genetic diversity. The aim of this research was to study genetic diversity of PO cattle in smallholder farmer based on morfology and molecular markers. This research was conducted at breeding center in East Java and Central Java include Tuban, Lamongan and Blora regency, since June until December 2008. PO bull used were of 18 months until 24 months of age and cow of 24 months until 36 months of age or at first calving as much as 30 head each location. The variables measured were body weight, body length, withers height, hip height, shoulder point width, chest girth, chest depth, canon bone circumference, head length and head width. Data were analyzed using descriptive statistic, average analysis between subpopulations, discriminant and canonical analysis and mahalanobis distance was done by SAS DISCRIM procedure. Whole blood were collected to get the data of polymorphism DNA microsatellite using technology of PCR and electrophoresis using 6 microsatellite loci. Variable measured were number and size of alel, alel frequency, heterozygosity,genetic distance and fixation index (F-stat). Data were analyzed using analysis of Microsate Toolkit, GENEPOPV4, MSA (Microsatellite Analyser) and Mega 4. The result showed that Blora and Tuban subpopulation have genetic diversity which is relative lower compared to that of Lamongan. The genetic distance which was close presented by Tuban and Blora subpopulation while Lamongan and Tuban subpopulation show far genetic distance. The result of canonical analyses showed high correlation on shoulder point width, head width, body length, withers height and hip height variable so this five variable can be used as distinguishing variables among subpopulation. The result of molecular genetics analysis using microsatellite showed that highest frequency of alel presented by HEL9 locus at Tuban population so this loci have the high polymorfism. The genetic differentiation among subpopulation showed by differentiation FST value among six loci indicating the increasing of inbreeding in the three subpopulations. Key words: Morphological Diversity, Genetic Differentiation, PO Cattle

SELEKSI PADA SAPI ACEH BERDASARKAN UJI PERFORMANS DI BPTU-HPT SAPI ACEH INDRAPURI Widya Pintaka Bayu Putra; Sumadi Sumadi; Tety Hartatik; Hendra Saumar
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 3, No 2 (2016): JITRO, Mei
Publisher : Universitas Halu Oleo

Uji performans merupakan tahap seleksi awal untuk memilih calon pejantan dan induk yang terbaik. Penelitian ini bertujuan untuk mengevaluasi pertumbuhan pada sapi Aceh dewasa. Sebanyak 49 ekor sapi Aceh (23 jantan dan 26 betina) digunakan untuk uji performan selama 369 hari di BPTU-HPT Sapi Aceh Indrapuri. Rata-rata umur sapi sebelum diuji adalah sekitar 600 hari dengan berat awal terkoreksi (BAT) sebesar 110,08+16,25 kg (jantan) dan 108,25+21,08 kg (betina). Rata-rata berat akhir terkoreksi (BFT) sebesar 138,30+16,59 kg (jantan) dan 141,63+24,88 kg (betina). hari (betina). Rata-rata tinggi gumba (TG), panjang badan (PB) dan lingkar dada (LD) setelah pengujian pada sapi jantan masing-masing sebesar 99,07+4,64 cm, 94,41+6,21 cm dan 120,85+6,41 cm. Selanjutnya rata-rata nilai TG, PB dan LD pada sapi betina setelah pengujian sebesar 97,00+4,00 cm, 95,62+4,90 cm dan 124,25+9,38 cm. Nilai BFT tertinggi selama pengujian sebesar 166,71 kg (jantan) dan 214,19 kg (betina). Kata kunci: Sapi Aceh, uji performans, berat badan, ukuran tubuh

The Application of Polymerase Chain Reaction – Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island Tety Hartatik; Slamet Diah Volkandari; S. Sumadi; W. Widodo
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. Samples used for the experiments were blood of 43 cattle that consist of 15 cattle obtained from Madura Island, 23 cattle from Sapudi Island, and 5 Limousin-Madura (Limura) cattle. A fragment of 464 base pair of cytb gene was amplifi ed by forward primer L14735 and reverse primer H15149. The PCR product was digested with TaqIand HinfI restriction enzymes to identify genetic patterns. Data of PCR-RFLP showed two haplotypes, that were A and B, in cattle obtained from both Madura Island and Sapudi Island. The frequencies of haplotype A and B of cattle from Sapudi Island were 69.57% and 30.47%, respectively. More diverse frequencies were observed in cattle obtained from Madura Island, where haplotype A and B were 86.67% and 13.33%, respectively. In this experiment, Limura cattle had only haplotype A. As a conclusion, PCR-RFLP of the cytb gene had been able to determine a genetic diversity of Madura cattle. Key words: Genetic diversity, Madura cattle, haplotype.

Identification of single nucleotide polymorphisms in GDF9 gene associated with litter size in Garut sheep Resti Yuliana Rahmawati; Sumadi Sumadi; Tety Hartatik
Indonesian Journal of Biotechnology Vol 24, No 1 (2019)
Publisher : Universitas Gadjah Mada

The growth differentiation factor 9 (GDF9) gene has been regarded as having major impacts on ovulation rate and litter size in sheep. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) of the GDF9 gene and their association with litter size in Garut sheep. For this purpose, a total of 60 ewes of Garut sheep were included in this study. Based on the sheep GDF9 reference sequences (Genbank Acc. No. AF078545.2), one pair of primers (5’-CTGCTGTTTAACCTGGATCGTG-3 5’-GGAGAGCCATACCGATGTCC-3 as forward and reverse, respectively) was used for PCR amplification. The results revealed that four SNPs (g.54C>T, g.60G>A, g.304G>A, and g.333G>A) were found in Garut sheep by direct sequencing. For SNP g.54C>T, the sheep exhibited the highest frequency of allele C and genotype CC. On the other hand, SNPs g.60G>A, g.304G>A, and g.333G>A showed a higher frequency of allele G than allele A, and the GG genotype was predominant in the population. SNP g.333G>A had a significant effect on litter size (p < 0.05), and ewes with the GG genotype had a higher litter size than those with the GA genotype. Genotype distributions for all identified SNPs were in agreement with Hardy-Weinberg equilibrium. We highlight that SNP g.333G>A may be useful as a genetic marker for litter size in Garut sheep.

Estimasi Parameter Genetik Sifat Pertumbuhan Kambing Boerawa di Kabupaten Tanggamus Propinsi Lampung (Genetic Parameters Estimation on Growth Traits of Boerawa Goat at Tanggamus Regency Lampung Province) Veronika Yuneriati Beyleto; Sumadi (Sumadi); Tety Hartatik
Buletin Peternakan Vol 34, No 3 (2010): Buletin Peternakan Vol. 34 (3) Oktober 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v34i3.82

This research was conducted to estimate heritability, repeatability and genetic correlation values on growth traits of Boerawa goat at Sumber Rejeki farmer group at Campang Village, Gisting District and Karya Makmur I and Karya Makmur II farmer group at Wonoharjo and Sukoharjo Village, Sumber Rejo District, Tanggamus regency, Lampung province. Data consisted of production record and 238 Boerawa goat derived from 93 PE and 7 Boer buck.The research was started on October 30, 2009 up to January 30, 2010. The results indicated that heritability value of birth weight, weaning weight, yearling weigth, average of preweaning daily gain, and average of postweaning weightanalyzed by paternal half-sibs correlation were 0.80±0.40; 0.30±0.17; 0.80±0.04; 0.32±0.18 and 0.30±0.17, respectively. The repeatability values of birth weight, weaning weight and yearling weight analyzed based on tworecording per every dam were 0.42±0.07; 0.32±0.08; 0.30±0.08; 0.30±0.08 and 0.53±0.06. The genetic correlation values among birth weight and weaning weight, birth weight and yearling weight, weaning weight and yearling weight,average of preweaning weight and postweaning weight daily gain analyzed by paternal half-sibs correlation were 0.50±0.04; 0.44±0.08; 0.21±0.03 and 0.20±0.05, respectively. The result also indicated that the heritability andrepeatability values was high and the genetic correlation values were moderate to high.(Key words: Boerawa goat, Genetic parameter, Growth character)

Karakteristik dan Kinerja Induk Sapi Silangan Limousin-Madura dan Madura di Kabupaten Sumenep dan Pamekasan (Characteristic and Performance of Limousin-Madura Grade and Madura Cows in Sumenep and Pamekasan Regencies) Tety Hartatik; Dhany Azharinto Mahardika; Tri Satya Mastuti Widi; Endang Baliarti
Buletin Peternakan Vol 33, No 3 (2009): Buletin Peternakan Vol. 33 (3) Oktober 2009
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v33i3.109

The research was conducted to investigate the characteristic and performance of Limura grade and Madura cows. The research was conducted in Sumenep and Pamekasan Regencies East Java Province within the period of May until August 2008. The method being used was an interview method on the farmer as respondents, continued by observing the exterior characteristics and the cattle measurements. The data of exterior characteristic were analyzed bydescriptive patterns. The data of vital measurements of the body were analyzed by independent sample t-test. The result showed that the general characteristics Limura crossbred cows had a dominant color of a reddish brown, with thebuttock color was a reddish brown. The general characteristics Madura cows had a dominant color of a light red, with the buttock color was a white smear. The vital characteristics of Limura crossbred cows was higher than Madura cows,at the 2 to 4 years old group cows the result showed that girth of chest, height at hip of Limura crossbred cows were 172.63±2.33 cm; 125.07±1.40 cm. while those of Madura cows were 140.81±3.01 cm; 114.77±1.29 cm. At older than 4years old group cows the result showed that heart girth, height at hip of Limura crossbred cows were 172.85±4.12 cm; 126.15±1.84 cm. while those of Madura cows were 157.57±2.55 cm; 118.54±1.53 cm. The performance of Limuracrossbred cows and Madura cows related to calving interval were 15.90±0.47 vs 14.39±0.23 months. The result showed that the general characteristics Limura crossbred cows and Madura cows had a different color at the dominant color ofbody and the buttock color, and Limura crossbred cows had a vital characteristics size higher than Madura cows, but the Madura’s reproductive performance showed better than Limura crossbred cows.(Key words: Characteristics, Performance, Limura crossbred cows, Madura cows)

Identifikasi Karakteristik Genetik Sapi Peranakan Ongole di Peternakan Rakyat (The Identification of Genetic Characteristic of Ongole Grade Cattle in Smallholder Farmers) Hartati (Hartati); Sumadi (Sumadi); Tety Hartatik
Buletin Peternakan Vol 33, No 2 (2009): Buletin Peternakan Vol. 33 (2) Juni 2009
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v33i2.118

The aim of this research was to identity genetic characteristic of Ongole Grade cattle and to analyse the diversity of Ongole grade cattle in smallholder farmers. This research was conducted at breeding stock in East Java and Central Javainclude Tuban, Lamongan and Blora regencies, since June until December 2008. The animal use were PO cattle of 18 months until 24 months of age and cow of 24 months until 36 months of age or have once of calving as many 30 headfrom location, were used as sampling for the observation of genetic diversity which was based on quantitative and qualitative characteristic. Whole blood collection was conducted to get the data of polymorphism DNA microsatelliteby using technology of PCR and elektroforesis. Data and information were analysed in descriptive, explanatory and laboratoris. The result showed that Blora subpopulation had genetic distance which was close to Tuban subpopulationcompared to Lamongan. The result of molecular genetic analyses by using microsatellite showed that highest frequency alel was presented by HEL9 locus at Tuban population. Heterozigosity value at 3 subpopulation of PO cattle was verylow, representing an indication of leading to inbreeding cases.(Key words : Genetic characteristic, PO cattle, Smallholder farmers)

Co-Authors Agus Susilo Aji, Riyan Nugroho Akhmad Fathoni Ali Agus Ali Agus Ali Agus Bayu Dewantoro Putro Soewandi Budi Prasetyo Widyobroto BUDI SETIADI DARYONO Dhany Azharinto Mahardika Diah Tri Widayati Dwi Aris Agung Nugrahaningsih Dwi Astuti Eko Purnomo Endang Baliarti Fatimah Az Zahra Chairunissa Hartati (Hartati) Hartati . Hartati . Hendra Saumar Hendra Saumar Hendra Saumar Hendry T.S.S.G. Saragih I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria Ismaya Ismaya Ismaya Ismaya Joko Riyanto Lies Mira Yusiati Nur Arfian Priyadi, Dwi Ahmad Resti Yuliana Rahmawati Ronny Martien S. Sumadi Sigit Bintara Slamet Diah Volkandari Slamet Diah Volkandari, Slamet Diah Slamet Diah Vulkandari Subandriyo . Sumadi (Sumadi) Sumadi . Sumadi . Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Tri Satya Mastuti Widi Trifena (Trifena) Utin Elsya Puspita Veronika Yuneriati Beyleto Wayan Tunas Artama Widodo Widodo Widya Pintaka Bayu Putra Widya Pintaka Bayu Putra Widya Pintaka Bayu Putra Widya Pintaka Bayu Putra Widya Pintaka Bayu Putra Widya Wasityastuti Yoga Cipta Perdana Yudi Adinata

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