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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak Veteriner
R Handarini
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Agriculture, Biological Sciences & Forestry Veterinary

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Viability of Timor deer stag (Cervus timorensis) spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature Mesang-Nalley, W. Marlene; Handarini, R; Purwantara, B
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 4 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.943 KB) | DOI: 10.14334/jitv.v12i4.499

Abstract

The successful  sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in  Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydrates  in Tris egg yolk (TEY) extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC). The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots.  Each of them was diluted with TEY-glucose (TEYG), TEY-fructose (TEYF) and TEY-Sucrose (TEYS) with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 ± 0.63 ml, pH 7.03±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 ± 7.4%. The average of sperm concentration was 842.35 ± 258.14x106 ml-1, the viable sperm was 78.11 ± 3.61%, the sperm abnormality was 7.31 ± 2.98%. The percentages of sperm motility on TEYG (18.00 ± 17.63%) and TEYS (21.83 ± 15.92%) were higher compare to TEYF (4,00 ± 0,00%) extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 ± 20.06) and TEYS (24.00 ± 22.59%) (P<0.05) were significantly higher compare to TEYF (4.00 ± 0.00%).  It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be used  for artificial insemination after 12 hour storage. Key Words: Liquid Semen, Deer, Room Temperature, Carbohydrate
Deer Frozen Semen Quality in Tris Sucrose and Tris Glucose Extender with Different Glycerol Concentrations W M. M Nalley; R Handarini; R I Arifiantini; T L Yusuf; B Purwantara; G Semiadi
Media Peternakan Vol. 34 No. 3 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.633 KB) | DOI: 10.5398/medpet.2011.34.3.196

Abstract

In order to improve Timor deer (Cervus timorensis) frozen semen quality, the influence of sugar and glycerol concentration on semen characteristics of sperm was investigated.  The semen was collected from five sexually mature Timor deer using an electroejaculator. The semen was evaluated and divided into six equal tubes and diluted with Tris sucrose glycerol 10% (TSG10); Tris sucrose glycerol 12% (TSG12); Tris sucrose glycerol 14% (TSG14); Tris glucose glycerol 10% (TGG10); Tris glucose glycerol 12% (TGG12); and Tris glucose glycerol 14% (TGG14). The diluted semen was packed in 0.3 ml minitub straw, equilibrated at 5 oC for 4 hours and frozen on liquid nitrogen vapor for 10 minutes. The total of forward motility, viability, acrosome integrity and membrane integrity were assessed in fresh, after equilibration and after thawing. Results demonstrated that the percentage of sperm motility in TSG10was higher (P (63.93±7.23%). The sperm in TSG10 and TSG14  extender were superior in acrosome as well as in membrane integrity. It was concluded that Tris Sucrose with 10% glycerol protected Timor deer sperm better than other combinations.
Viability of Timor deer stag (Cervus timorensis) spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature W. Marlene Mesang-Nalley; R Handarini; B Purwantara
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.943 KB) | DOI: 10.14334/jitv.v12i4.499

Abstract

The successful  sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in  Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydrates  in Tris egg yolk (TEY) extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC). The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots.  Each of them was diluted with TEY-glucose (TEYG), TEY-fructose (TEYF) and TEY-Sucrose (TEYS) with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 ± 0.63 ml, pH 7.03±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 ± 7.4%. The average of sperm concentration was 842.35 ± 258.14x106 ml-1, the viable sperm was 78.11 ± 3.61%, the sperm abnormality was 7.31 ± 2.98%. The percentages of sperm motility on TEYG (18.00 ± 17.63%) and TEYS (21.83 ± 15.92%) were higher compare to TEYF (4,00 ± 0,00%) extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 ± 20.06) and TEYS (24.00 ± 22.59%) (P<0.05) were significantly higher compare to TEYF (4.00 ± 0.00%).  It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be used  for artificial insemination after 12 hour storage. Key Words: Liquid Semen, Deer, Room Temperature, Carbohydrate
Co-Authors B Purwantara B Purwantara G Semiadi R I Arifiantini T L Yusuf Wilmientje Marlene Mesang Nalley