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Co-Culture Anoxygenic Photosynthetic Bacteria With Bacillus sp. Isolated From Hanura Beach Against Vibrio sp. Sumardi Sumardi; Salman Farisi; Christina Nugroho Ekowati; Sundari Ayu Oktalia
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.43

Abstract

A mangrove forest ecosystem is a place where many special Bacillus sp. found. This study aims to obtain Bacillus sp. isolates that can inhibit the growth of Vibrio sp. This study was conducted by selecting 77 Bacillus sp. isolates from the Hanura mangrove forest area. In addition, selected bacteria were tested for salt stress, pH stress, pathogenicity test, and antibiotic susceptibility test. Selected isolates were cultivated together with Vibrio sp. for 7 days. Bacillus sp. and Vibrio sp. cultured again with the addition of anoxygenic photosynthetic bacteria for 4 days. From the results of the initial selection, 4 isolates could inhibit the growth of Vibrio sp. The final results showed that Bacillus IL2K8 isolates were known to inhibit the growth of Vibrio sp., can grow at salinity 0%, 3%, 6% and pH 7 and pH 10. IL2K8 isolates are not pathogenic, resistant to amplicin antibiotics, streptomycin, nalidixic acid, and chloramphenicol but sensitive to trimethoprim antibiotics. In the joint culture of liquid media IL2K8 together with Anoxygenic Photosynthetic Bacteria (APB) isolates IL2K8 can inhibit the growth of Vibrio sp.
Proteolytic Activity of The Entomopathogenic Fungi (Penicillium sp. ) of Cockroaches (Periplaneta americana) Ahmad Ikhsanudin; Emantis Rosa; Christina Nugroho Ekowati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.47

Abstract

Cockroaches (Periplaneta americana) were the insect vectors of disease that caused adverse effects on human health. Control cockroch excessive use of insecticides can lead to residus in the environment and resistance cockroach. Therefore it was necessary to use an alternatives such as such as entomopathogenic fungal as biologycal agents. The entomopathogenic fungi penetrated via the integument of an cockroach to reach the hemocoel. Proteins were the molecules responsible for integument strength in cockroach, It was synthesis the proteases to degrading proteins. The study begins with the isolation of entomopathogenic fungi using the moist chamber method with cockroach as insect bait. Fungus that grow on cockroches are cultured and purifed on Potato Dextrose Agar (PDA) medium then identified. Identification was carried out through macroscopic observations including colony color and diameter and microscopic observations including conidia, conidiophores, hyphae, vesicles, fialids, and leg cells. The result of isolation and identification obtained as Penicillium sp. Proteases enzimatic activity tested on PDA with anlene 1%. The clear zone formed is measured to show the activity of proteases produced by Penicillium sp.
Pelatihan Pembuatan Herbarium Sebagai Pengayaan Media Pembelajaran Ipa-Biologi Bagi Guru Smp Mgmp Ipa Di Kecamatan Way Tenong Kabupaten Lampung Barat Sumardi Sumardi; Tundjung Tripeni Handayani; Christina Nugroho Ekowati
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 3 (2020): Peran Perguruan Tinggi dan Dunia Usaha Dalam Pemberdayaan Masyarakat Untuk Menyongsong
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.2 KB) | DOI: 10.37695/pkmcsr.v3i0.852

Abstract

Sebagian besar guru SMP IPA di Kecamatan Way Tenong bukan dari latar belakang Pendidikan Biologi, namun tetap harus dapat mengajarkan materi tentang Biologi. Dalam mengajar Biologi mereka sering menemui kendala, yaitu kesulitan mendapatkan bahan yang sesuai. Untuk menanggulangi hal tersebut dapat digunakan objek awetan antara lain herbarium. Pelaksanaan Pelatihan dilakukan dengan pendekatan pemberian teori dan praktek langsung membuat herbarium. Pelatihan diikuti oleh 31 orang guru SMP MGMP IPA Lampung Barat. Sebelum pelatihan dilaksanakan , pengetahuan peserta tentang pembuatan herbarium sebesar 44,5%. Setelah pelatihan pengetahuan peserta meningkat menjadi 93,2%. Dengan demikian terjadi peningkatan pengetahuan sebesar 48,7%. Hasil Pelatihan, berupa herbarium dapat digunakan sebagai media pembelajaran bidang IPA khususnya Biologi.