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Efek Antimikroba Ekstrak Lidah Buaya (Aloe vera) Terhadap Isolat Bakteri Penyebab Acne vulgaris Secara Invitro Bahar, Meiskha; Yusmaini, Hany
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2017): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.152 KB) | DOI: 10.33533/jpm.v11i2.222

Abstract

Acne vulgaris adalah suatu kondisi inflamasi umum pada unit polisebaseus, ditandai dengan komedo, papul, pustul atau nodul. Penyakit kulit ini bukan merupakan penyakit yang berbahaya tetapi mempunyai dampak yang besar secara fisik maupun psikologik. Prinsip penanganan acne antara lain  menurunkan populasi Propionibacterium acne dan menekan inflamasi. Dari penelitian sebelumnya ditemukan adanya mikroorganisme lainnya dalam lesi yang mungkin berperan selain Propionibacterium acne yaitu Staphylococcus aureus, S. epidermidis dan Pityrosporum ovale. Beberapa sumber melaporkan ada beberapa manfaat Aloe vera untuk kecantikan dan perawatan kulit. Aloe vera juga digunakan secara eksternal untuk mengobati berbagai kondisi kulit seperti luka, nyeri dan menekan proses inflamasi. Penelitian ini dilakukan untuk mengetahui efek antimikroba ekstrak Aloe vera konsentrasi 25%,50% dan 75% terhadap isolat bakteri penyebab Acne vulgaris secara invitro dengan menggunakan metode difusi. Sebelum pengujian dilakukan isolasi dan identifikasi bakteri dari lesi. Bakteri yang ditemukan dari lesi penderita tergolong bakteri golongan Gram positif yaitu  Staphylococcus aureus dan Propionibacterium acne. Hasil uji One-way Anova  menunjukan terdapat perbedaan bermakna efektivitas ekstrak lidah buaya terhadap S.aureus dan P.acne. Uji Post Hoc terhadap S.aureus  menunjukan terdapat perbedaan bermakna ELB 25%, 50% dan 75% dengan kontrol (+) dan kontrol (-), tidak terdapat perbedaan bermakna antara ELB 25% dengan 50% dan 50% dengan 75%. Sedangkan antara 25% - 75% berbeda bermakna. Sedangkan Uji Post Hoc terhadap P.acne menunjukan bahwa tidak terdapat perbedaan bermakna antara  ELB 25%, 50% dan 75% dengan kontrol positif. Kesimpulan : Ekstrak Lidah Buaya (Aloe vera)  mempunyai efek antimikroba terhadap isolat bakteri penyebab Acne vulgaris yaitu Propionibacterium acne dan Staphylococcus aureus  pada konsentrasi 25%, 50% dan 75% secara invitro.
UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) TERHADAP ISOLAT BAKTERI Escherichia coli JAJANAN CILOK SECARA IN VITRO DENGAN METODE DIFUSI Abima, Fadel; Bahar, Meiskha; Chairani, Aulia
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2017): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.317 KB) | DOI: 10.33533/jpm.v11i1.205

Abstract

Diarrhea is still one of the world’s major health problem especially in developing country. Foods and beverages contaminated by microorganism become the risk factor of diarrhea, including Escherichia coli (E. coli). One of the curative effort that can be done is to utilise the secondary metabolite compounds contained in binahong leaf (Anredera cordifolia (Ten.) Steenis). This study used true experimental method. The sample was binahong leaf extract with concentration of 25%, 50, and 75% using diffusion method (Kirby-Bauer) on doublelayered Mueller Hinton Agar (MHA) (base layer and seed layer) in order to measure the growth inhibition zone around the cylinder plate. The amount of population on this study was 24 isolations of E. coli. The repetition of each treatment group was counted by Federer formula. The result analyzed using Kruskal-Wallis test and showed that there was difference on binahong leaf extract effectiveness (p < 0,05), as well as on post hoc analysis using MannWhitney test showed that there was significant difference at concentration of 25%,50%, and 75%. (p < 0,001). This shows that there is a significant differences in the concentration of binahong leaf extract.
PERBANDINGAN EFEKTIVITAS ANTARA MINYAK CENGKEH (Syzigium aromaticum) DAN LARUTAN OBAT KUMUR YANG MENGANDUNG DAUN SIRIH DALAM MENGHAMBAT PERTUMBUHAN MIKROORGANISME PEMBENTUK PLAK GIGI SECARA IN VITRO Bahar, Meiskha; Sudomo, Pertiwi
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 9, No 1 (2015): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4129.312 KB) | DOI: 10.33533/jpm.v9i1.825

Abstract

Dental plaque is formed by colony of bacteria attached to the tooth’s smooth surface. Plaque on the tooth caused by the type of food consumed and the activity of thousands of microorganisms. The main microorganisms that forming dental plaque is Streptococcus mutans. If plaque is not removed daily by brushing it will become a hard tartar. Dental plaque can be removed by brushing or flossing between teeth or use mouthwash solution containing antimicrobial agents. One of the types of plant that has the ability to inhibit and kill the microorganisms is clover (Syzigium aromaticum). The aim of this study is to determine the effectiveness of bioactive compounds contained in clove oil isolated microorganisms from dental plaque and the compared with the mouthwash solution containing betel leaf in inhibiting the growth of the bacteria. This study was an experimental research approach post test only control group design with 40 isolates of dental plaque bacteria samples which were selected to find out the type of S.mutans. The result showed that clove oil concentration of 10%; 20%; 30%; dan 40%, have the ability to inhibit S. mutans as well as the other 39 isolates of dental plaque dental bacteria. Based on the analysis of data from the one-way ANOVA test showed significance of 0.05 which means there was no significant difference in the inhibition of clove oil againts dental plaque bacteria isolates of S. mutans and the other 30 isolates of dental plaque in various concentrations.
Potensi Antibakteri Isolat Actinomycetes terhadap Aktivitas Proteolitik dan Amilolitik Escherichia Coli ATTC 25922 Bahar, Meiskha; Zulfa, Fajriati
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (546.699 KB) | DOI: 10.29238/teknolabjournal.v7i1.101

Abstract

Occurs E. coli resistance to class 3 cephalosporin class antibiotics and fluoroquinolone groups. The antibiotic resistance that occurs has narrowed the choice of therapy. This study aims to determine the effect of Actinomycetes isolates on proteolytic and amylolytic enzymes E. coli ATCC 25922. This research is experimental research, qualitative tests of protease and amylase enzymes from E. coli ATCC 25922 shown by clear zones around the growing colonies. The result of ANOVA One-Way test showed a significant difference in the width of clear zone, colony zone and PER and AER score with p-value &lt; 0,05. This indicates that Actinomycetes isolates contain compounds that can act as inhibitors of protease and amylase enzymes from E.coli ATCC 25922. It is hoped that there will be research about the identification of Actinomycetes species isolates in Bogor Botanical Garden so that later can be cultivated and produced as an antibiotics alternative.
MANFAAT ASAM FOLAT UNTUK MENCEGAH OROFACIAL CLEFT PADA KOMUNITAS SATU SENYUM Fauziah, Cut; Thadeus, Maria Selvester; Bahar, Meiskha; Sukrisno, Adi; Zulfa, Fajriati
Jurnal Pengabdian Masyarakat AbdiMas Vol 8, No 03 (2022): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v8i03.5185

Abstract

Kasus bayi lahir dengan bibir sumbing masih banyak terjadi di Indonesia. Bibir sumbing merupakan kelainan berupa celah pada bibir atas. Celah ini bisa terjadi pada bagian langit-langit rongga mulut (cleft palate), bisa juga pada bagian bibir saja (cleft lip). Di beberapa kasus juga bisa terjadi pada kedua bagian. Namun pada umumnya, hampir separuh kasus bibir sumbing melibatkan celah pada bibir atas serta atap rongga mulut. Pemberian asam folat pada ibu hamil bertujuan untuk mencegah terjadinya kelainan kongenital yang terjadi selama masa perkembangan embryo , khususnya di trimester pertama. Kelainan kongenital yang selama ini berhubungan dengan kondisi folat ibu adalah Neural Tube Defect. Penelitian penelitian selanjutnya juga menyatakan bahwa ternyata asam folat juga berpengaruh terhadap kegagalan pembentukan jantung yang sempurna, kejadian celah bibir dan/atau langit. Metode penyuluhan dilakukan dengan metode ceramah secara daring via zoom, menggunakan slide power point tentang manfaat asam folat bagi ibu ibu anggota komunitas yang masih ingin memiliki anak dan tentang mempersiapkan kehamilan yang sehat dan menghadapi seribu hari pertama kehidupan. Keberhasilan dari kegiatan ini adalah terpenuhinya target dari jumlah peserta, serta ketercapaian tujuan dari kegiatan penyuluhan ini juga dapat dikatakan baik dari hasil pretest dan postest dan ketercapaian target materi  (100%).  Kesimpulan  dari  kegiatan  ini  adalah  terjadi peningkatan pengetahuan pada  ibu ibu anggota komunitas dapat  tentang manfaat asam folat untuk pencegahan orofacial cleft pada kehamilan berikutnya. Kata Kunci: Asam folat, orofacial cleft, komunitas
Uji Daya Hambat Filtrat Zat Metabolit Lactobacillus plantarum Terhadap Pertumbuhan Shigella dysenteriae Secara In Vitro Rizki Putri Andini Rahmah; Meiskha Bahar; Yanti Harjono
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3431

Abstract

Shigella dysenteriae is pathogenic bacteria causes dysentery diarrhea, a diarrhea with mucus and blood in the feces. Currently, drugs or foods containing Lactobacillus have been circulated in the markets and used by people. This research aims to know the in vitro inhibition test of L. plantarum metabolite substances filtrate against growth of S. dysenteriae. This research used experimental design with L. plantarum metabolite substances filtrate as the samples. Antibacterial test method which was used was the cup-plate technique by looking at clear zone of bacteria’s growth around cup-plate and used Mueller Hinton Agar media. The results showed clear zones on filtrate with concentrations 25%, 50%, 75%, and 100% with average inhibition zones 0,284 cm; 0,332 cm; 0,406 cm; and 0,619 cm. Kruskal-Wallis test results indicated there were significant differences of each filtrate concentration against S. dysenteriae (p<0,05). Post Hoc test showed no significant difference between concentrations 25% and 50% (p>0,05). This suggested lactic acid, acetic acid, and bacteriosin presented in L. plantarum metabolite have antibacterial characteristic
Uji Efektivitas Antibakteri Perasan Jus Buah Nanas (Ananas comosus) Terhadap Pertumbuhan Isolat Bakteri Plak Gigi di Puskesmas Kecamatan Tanah Abang Periode April 2017 Mitta Nurfitri Saridewi; Meiskha Bahar; Anisah Anisah
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 2 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i2.3532

Abstract

Dental caries or commonly known as “Tooth Decay” is one of the most common infection on human. Dental caries begins by the formation of dental plaque and it is a multifactorial disorder. One of the efforts to prevent the formation of dental caries is by utilizing plants which contain antibacterial substance. Pineapple contains bromelain, saponin, flavonoid, and tannin enzymes which functions as antibacteria. This research aimed to discover the antibacterial effectiveness of pineapple juice toward the isolate growth of dental plaque bacteria by using three different pineapple juice concentrations (25%, 50%, and 75%). The method was experimental by using dental bacteria isolate from a patient of Dental and Oral Clinic in Puskesmas Kecamatan Tanah Abang. The result shows that the higher concentration shows the bigger inhibition zone diameter formed around the plate’s paper. Consequently in concentrations of 25%, 50%, and 75%, each has its own inhibition zone rate as much as 2,90 mm (weak); 3,89 mm (weak); and 5,89 mm (moderate).
Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro Saskia Arientika Wahyuningrum; Meiskha Bahar; Andri Pramesyanti Pramono
Jurnal Kesehatan Andalas Vol 10, No 1 (2021): Online March 2021
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v10i1.1595

Abstract

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa
Nicotiana tabacum var. Virginia Bio Oil-based Pyrolysis Extraction Have Prominence Antimicrobial Potential Compared to Ethanol Heat Reflux Extraction (EHRE) Andri Pramesyanti Pramono; Basra Ahmad Amru; Halimah Anggi Rahmani; Sheila Azelya Fernanda; Yudhi Nugraha; Muhammad Yusuf Arya Ramadhan; Andre Fahriz Perdana Harahap; Ahmad Fauzantoro; Nasihin Saud Irsyad; Meiskha Bahar; Oktania Sandra Puspita; Fajriati Zulfa; Kori Yati; Mahdi Jufri; Misri Gozan
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.515-525

Abstract

Tobacco leaf contains antibacterial secondary metabolite compounds, such as phenol, alkaloids, and essential oils. This study compares the potential antibacterial effects of Indonesian tobacco leaf extracted using the heat reflux method (producing an extract) and pyrolysis method (providing a bio-oil). The tobacco leaf extract was challenged against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853. The bio-oil from the pyrolysis method showed inhibitory Kirby Bauer zones higher than those of the extract from heat reflux method, with the maximum results in the pyrolysis method indicating zones of 6.35 mm (S. aureus), 5.90 mm (E. faecalis), 3.97 mm (E. coli), and 5.025 mm (P. aeruginosa). Further study analyzed the effectiveness of the disc and well diffusion antibacterial test methods for measuring the antibacterial effect of bio-oils against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. The bio-oil used in the well diffusion test method showed the most significant antibacterial effectiveness. It showed the biggest inhibition zone, with a maximum of 11.65 mm and 8.90 mm for E. coli and P. aeruginosa. Our results showed Nicotiana tabacum var. Virginia Bio Oil from Ponorogo (Indonesia) is a strong potential antimicrobial, especially using well diffusion test.
PENGARUH MADU TERHADAP TINGKAT NYERI DISMENORE DAN KUALITAS HIDUP MAHASISWI FAKULTAS KEDOKTERAN UNIVERSITAS PEMBANGUNAN NASIONAL VETERAN JAKARTA Nurfitri - Bustamam; Cut - Fauziah; Meiskha - Bahar
Jurnal Kesehatan Reproduksi Vol 12 No 1 (2021): JURNAL KESEHATAN REPRODUKSI VOLUME 12 NOMOR 1 TAHUN 2021
Publisher : Puslitbang Upaya Kesehatan Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/kespro.v12i1.3914

Abstract

Latar belakang: Prevalensi dismenore diperkirakan berkisar antara 45–95 persen dan 10–25 persen diantaranya merupakan dismenore primer berat. Dismenore dapat menimbulkan sejumlah masalah, antara lain limitasi aktivitas, penurunan prestasi akademik, dan kesulitan tidur. Saat ini, terdapat kecenderungan penggunaan herbal dan pengobatan alternatif untuk mengatasi dismenore primer. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh madu terhadap tingkat nyeri dismenore dan kualitas hidup mahasiswi Fakultas Kedokteran Universitas Pembangunan Nasional Veteran Jakarta (FKUPNVJ). Metode: Penelitian menggunakan one group pretest-posttest design. Sebanyak 30 subjek yang ditentukan dengan consecutive sampling diminta minum madu sebanyak dua sendok makan yang dimulai dari dua hari sebelum menstruasi hingga hari ketiga menstruasi. Data diambil menggunakan kuesioner, Numeric Rating Scale, Verbal Multidimensional Scoring System, dan Brief Pain Inventory. Hasil: Hasil uji Wilcoxon menunjukkan madu dapat menurunkan intensitas nyeri (p = 0,000) dan grade dismenore (p = 0,001). Selain mengurangi derajat nyeri, madu dapat mengurangi lama waktu nyeri menstruasi dari 2 hari menjadi 1 hari (p = 0,001). Hasil uji Wilcoxon juga menunjukkan madu dapat mengurangi gangguan dismenore terhadap aktivitas secara umum, suasana hati, kemampuan berjalan, pekerjaan, hubungan dengan orang lain, tidur, dan menikmati hidup (p ≤ 0,001). Kesimpulan: madu dapat menurunkan tingkat nyeri dan meningkatkan kualitas hidup mahasiswi FKUPNVJ dengan dismenore primer. Kata kunci: dismenore primer, kualitas hidup, madu, mahasiswi