Garuda - Garba Rujukan Digital

Daya Antioksidan Ekstrak Kulit Buah Naga Dan Sabun Ekstrak Kulit Buah Naga (Hylocereus Polyrhizuz) Mohamad Ikram; Nining Sugihartini; Any Guntarti
Jurnal Surya Medika (JSM) Vol 6 No 1 (2020): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsm.v6i1.1616

Red dragon fruit peel extract contains betalain which has antioxidant activity. In addition, this extract also contains dyes that can be used as coloring agents in cosmetic preparations. The purpose of this study was to study the activity of dragon fruit peel extract and red dragon fruit peel extract soap with various concentrations. Antioxidant activity is taken by the DPPH method. The concentration of red dragon fruit peel extract in soap is 0.3; 0.6; 0.9%. Test results show red dragon fruit extract soap has lower antioxidant activity than the extract. The increased concentration of the extract caused an increase in the antioxidant activity of the red dragon fruit peel extract soap.

Analysis of dog fat in beef sausage using FTIR (Fourier Transform Infrared) combined with chemometrics Any Guntarti; Zelinda Ayu Purbowati
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Sausage is ready to eat meals for children and adults. The meat contained in a beef sausage might not be consistent to the one listed on the label. FTIR (Fourier Transform Infrared) spectroscopy combined with a chemometric method was the most used methods to detect beef fat with rapid and consistent results. Results of analysis can classify fatty acid composition. The aim of this study was to know the profile of spectra and grouping of beef tallow and dog meat with FTIR. The study was designed by making 7 different variations of dog reference sample concentrations (100%, 75%, 65%, 50%, 35%, 25%, and 100%) beef fat and five other samples were gathered from various street vendors. Results were analyzed using FTIR spectroscopy combined with chemometric with PLS (Partial Least Square) and PCA (Principal Component Analysis). The results of analysis will be analysed using Horizon MB application to obtained optimal wave number at 688-1124 cm-Â¹. Results equation obtained y = 0.9999 x + 0.0004 and value RÂ² equal to 0.9999; RMSEC (root mean square error of calibration) of 0.30%; RMSEP (root mean square error of prediction) of 0.05% and RMSECV (root mean square error of cross validation) of 0.05%. The low values of RMSEP, RMSEC, RMSECV and RÂ² close to one indicate that the FTIR combined with chemometric is an appropriate method for analyzing the presence of fat content in the sample. From the analysis showed that 1 of 5 sausage market samples has similarities with dog fat.Â

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK GANGGANG HIJAU (Ulva lactuca L.) DENGAN VARIASI KONSENTRASI PELARUT ETANOL 60%, 75%, DAN 96% MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil) Desti Kameliani; Nina Salamah; Any Guntarti
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 5 No 2 (2020): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Free radicals give a negative effect. According to earlier research, Spyrogyra and Ulva lactuca L. have an anti-oxidant activity. This research aim to knowing the extract potential of green algae (Ulva lactuca) with variety concentration solvent as anti-oxidant by DPPH method, and finding the price of IC50 ethanol extract which compared with Galat acid. Extraction technic was maseration with ethanol solvent 60%,75%, and 96% which is implemented a qualitative test of extract active compound of U. lactuca Antioxidant activity of U. lactuca extract measured with spektrofotometri by using DPPH (1,1-difenil-2-pikrilhidrazil) method to get percentation of free radical resistor. The analytical result using SPSS, statistically got 95% credibility (p=0,05). This research showed that activity of antioxidant ethanol extract 96% of U. lactuca was higher than ethanol extract 60% and 75%. Activity of antioxidant ethanol extract 60%,75%, and 96% which confirmed by value of IC50 continously are 441,70 mg/ml ; 242,10 mg/ml dan 2,04 mg/ml. Whereas the value of IC50 galat acid standard is 3,92 x 10-3mg/ml. Based on tests of conducted antioxidant activity, the gallic acid has a strong antioxidant power, U. lactuca have a very weak antioxidant power

TOTAL FLAVONOID DETERMINATION AND ANTIOXIDANT ACTIVITY STUDY OF SPINACH (Amaranthus tricolor L.) RED GITI AND GREEN GITI VARIETY Any Guntarti; Anita Ruliyani
Jurnal Farmasi Sains dan Praktis Vol 6 No 1 (2020)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v6i1.3196

Antioxidants in the form of total flavonoids are compounds that can stabilize freeradicals. The mechanism of antioxidants in stabilizing free radicals is give the lackelectron of free radicals and inhibit the formation of free radical formation thatcan damage the body. DPPH method is used to determine the activity of capturingfree radicals of ethanol extracts of Amaranthus tricolor L. red giti and green giti.As a positive control quercetin is used. The results showed that the total amount offlavonoids in red giti spinach was (5.615±0.114)% EK and green giti spinach was(4.468±0.166)% EK. The results also show that all groups have activity as freeradical scavengers. The value of ES 50 for positive control quercetin, red gitispinach extract, and green giti spinach extract are (1.375±0.085)µg/ml,(186.198±0.363)µg/ml, and (209.392± 0.607)µg/ml respectively. Based on ES 50values It can be concluded that antioxidant activity of red giti spinach extract andgreen giti spinach extract are significantly different from the positive controlquercetin. Antioxidant activity of quercetin is categorized strong antioxidantactivity, while the antioxidant activity of red giti spinach extract and green gitispinach extract is categorized as weak antioxidant activity.

ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (BRASSICA OLERACEA L. VAR. CAPITATA F. RUBRA) AND WHITE CABBAGE (BRASSICA OLERACEA L. VAR. CAPITATA F. ALBA) ETHANOL EXTRACT USING DPPH METHOD (1,1-DIFENIL-2-PIKRILHIDRAZIL) Any Guntarti; Ratna Yuningtyas; Hari Susanti; Zainab Zainab
Jurnal Farmasi Sains dan Praktis Vol 7 No 2 (2021)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v7i2.4369

Antioxidants are compounds that are needed by the body to protect the body from free radical attacks that can trigger the emergence of degenerative diseases. One of the compounds that has free radical scavenger activity by inhibiting Reactive Oxygen Species (ROS) is flavonoids found in purple cabbage and white cabbage. This study aims to determine the total levels of flavonoids expressed as quercetin equivalent (EK) and antioxidant activity using the DPPH method (1,1-diphenyl-2- picrylhydrazyl). Identification of flavonoid compounds by Willstater test and determination of total flavonoid levels using spectrophotometry, AlCl3 reagent. Flavonoid levels were calculated using linear regression equations. Qualitative test for the presence of antioxidant activity using TLC and antioxidant activity test using the DPPH method with the parameter value of ES50. The qualitative test results showed that the ethanol extract of purple cabbage and white cabbage contained flavonoids. The total flavonoid content of ethanol extract of purple cabbage was 5.17% w/w (EK) and ethanol extract of white cabbage was 3.84% w/w (EK). Qualitative test with TLC showed antioxidant activity. The standard antioxidant activity values of quercetin, ethanol extract of purple cabbage and white cabbage were 2.138±0.064 µg/mL; 154.445±0,999 µg/mL and 373.546±1.336 µg/mL. The antioxidant activity of purple cabbage is weak and white cabbage is very weak.

ANALISIS PROFIL MINYAK DAUN CENGKEH (Syzygium aromaticum (L.) Merr. & L. M. Perry) DAN PRODUK DI PASARAN : PROFILE ANALYSIS OF CLOVE LEAF OIL (Syzygium aromaticum (L.) Merr. & L. M. Perry) AND PRODUCTS IN THE MARKET Any Guntarti; Putri Lestari; Laela Hayu Nurani
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 1 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i1.534

Minyak cengkeh memiliki nilai komersial dan manfaat yang besar serta permintaan ketersediaan minyak cengkeh di pasaran sangat tinggi, namun hasil ekstraksi minyak cengkeh cukup rendah sekitar 2-3% sehingga dapat menimbulkan motif untuk mengeksplorasi pasokan minyak daun cengkeh dengan tindakan pemalsuan. Tujuan dari penelitian ini untuk menganalisis secara kualitatif profil dan kemurnian minyak daun cengkeh dan produk minyak daun cengkeh di pasaran berdasarkan persyaratan mutu dari Standar Nasional Indonesia (SNI). Jenis penelitian ini berupa penelitian non eksperimental. Minyak daun cengkeh dan minyak produk A, B dan C dilakukan uji kualitas minyak berdasarkan syarat uji SNI 06-2387-2006 yang meliputi organoleptis (warna dan bau), bobot jenis, indeks bias dan kelarutan dalam etanol 70%. Selanjutnya dilakukan analisis KLT dengan menggunakan fase diam silika gel GF254 dan fase gerak Toluen : Etil Asetat (93:7). Hasil destilasi minyak daun cengkeh sebanyak 3 Kg dengan destilasi uap air didapatkan rendemen sebesar 0,83%. Hasil analisis profil minyak menunjukkan bahwa minyak daun cengkeh dan minyak produk A memenuhi syarat uji SNI 06-2387-2006, sedangkan pada minyak produk B dan C terdapat beberapa parameter pengujian yang tidak memenuhi syarat uji di antaranya bobot jenis, indeks bias dan kelarutan dalam etanol 70%, sedangkan pada hasil uji dengan KLT menunjukkan pola noda yang sama dengan nilai Rf sebesar 0,63.

ANALISIS PROFIL MINYAK DAUN CENGKEH (Syzygium aromaticum (L.) Merr. & L. M. Perry) DAN PRODUK DI PASARAN : PROFILE ANALYSIS OF CLOVE LEAF OIL (Syzygium aromaticum (L.) Merr. & L. M. Perry) AND PRODUCTS IN THE MARKET Any Guntarti; Putri Lestari; Laela Hayu Nurani
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 1 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i1.534

Minyak cengkeh memiliki nilai komersial dan manfaat yang besar serta permintaan ketersediaan minyak cengkeh di pasaran sangat tinggi, namun hasil ekstraksi minyak cengkeh cukup rendah sekitar 2-3% sehingga dapat menimbulkan motif untuk mengeksplorasi pasokan minyak daun cengkeh dengan tindakan pemalsuan. Tujuan dari penelitian ini untuk menganalisis secara kualitatif profil dan kemurnian minyak daun cengkeh dan produk minyak daun cengkeh di pasaran berdasarkan persyaratan mutu dari Standar Nasional Indonesia (SNI). Jenis penelitian ini berupa penelitian non eksperimental. Minyak daun cengkeh dan minyak produk A, B dan C dilakukan uji kualitas minyak berdasarkan syarat uji SNI 06-2387-2006 yang meliputi organoleptis (warna dan bau), bobot jenis, indeks bias dan kelarutan dalam etanol 70%. Selanjutnya dilakukan analisis KLT dengan menggunakan fase diam silika gel GF254 dan fase gerak Toluen : Etil Asetat (93:7). Hasil destilasi minyak daun cengkeh sebanyak 3 Kg dengan destilasi uap air didapatkan rendemen sebesar 0,83%. Hasil analisis profil minyak menunjukkan bahwa minyak daun cengkeh dan minyak produk A memenuhi syarat uji SNI 06-2387-2006, sedangkan pada minyak produk B dan C terdapat beberapa parameter pengujian yang tidak memenuhi syarat uji di antaranya bobot jenis, indeks bias dan kelarutan dalam etanol 70%, sedangkan pada hasil uji dengan KLT menunjukkan pola noda yang sama dengan nilai Rf sebesar 0,63.

THE APOPTOSIS INDUCTION OF Zingiber oficinale ETHANOLIC EXTRACT-Treated HeLa (HUMAN CERVICAL CANCER) CELLS AND ACTIVE COMPOUND PROFILING USING GAS CHROMATOGRAPHY/MASS SPECTROMETRY Laela Hayu Nurani; Siti Rofida; Dwi Utami; Citra Ariani Edityaningrum; Any Guntarti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 9 No 1 (2024)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v9i1.1059

Cervical cancer accounts for the highest percentage of cancer-related deaths in Indonesia, accounting for nearly 60% of all cancer cases. Therefore, research into the anticancer mechanisms needs to be conducted. The ethanolic extract of Zingiber officinale (EEZO) contains zingiberene, a chemical known for its anticancer activity. Understanding the mechanism underlying the apoptosis-inducing effects is crucial. This study aimed to elucidate the apoptotic pathway and analyze the gas chromatography/mass spectrometry (GC/MS) profile of EEZO cells. The research commenced with the maceration of Zingiber officinale rhizomes using 75% ethanol to obtain EEZO. Apoptosis assays were conducted on both a negative control group and an EEZO-treated group of HeLa cells (cervical cancer cells). The apoptotic mechanism was evaluated using forward scattered light-side scattered light (FSC-SSC), fluorescein isothiocyanate (FTIC), and phycoerythrin (PE) flow cytometry. Apoptotic results were analyzed by comparing the control and EEZO samples, which revealed the number of viable cells, apoptotic cells, and cells in the sub-G1 phase. The major constituent of EEZO, which is expected to be a potent apoptosis inducer, was detected using GC/MS. The FSC-SSC results indicated a lower number of viable cells in the EEZO-exposed group than in the control group. FTIC results demonstrated that EEZO significantly increased apoptotic cell death, increasing from 68 to 1537 cells. PE flow cytometry revealed an elevated sub-G1 cell population, indicating the induction of apoptosis by EEZO. GC/MS analysis revealed five dominant components in EEZO, which had the potential to induce apoptosis: L-borneol, zingiberene, farnesol, beta-sesquiphellandrene, and alpha-curcumene ...

Concentration of isolated DNA face masks made of gelatin for halal authentication Nazila Nur Hikmah; Any Guntarti; Nina Salamah
Journal of Halal Science and Research Vol. 5 No. 1 (2024): February
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v5i1.8951

The function of using a face mask is to remove dirt and dead skin cells found in the pores of the skin, open clogged pores and clean the remaining cosmetics that cannot remove with ordinary cleansers, repair and tighten the skin, provide nutrition, smooth, soften and maintain skin moisture. People are aware of halal-labeled cosmetics, one of which is face masks because some face masks contain gelatin. Some face masks on the market contain gelatin. Preparation of 5 reference samples with different concentrations. Preparation of face masks, DNA isolation, and checking the isolation results. The test results with the Nanodrop Spectrophotometer showed that the face mask DNA containing pig and cow gelatin extracted in this study ranged from 163.35 ng/µl in sample S6 to 83.550 ng/µl in sample S7. With the largest concentrations of 163.35 ng/µl and 83.550 ng/µl, while the highest purity value at the λ260/λ280 ratio was 1.41.

Title

Found 9 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search