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All Journal Journal of Food and Pharmaceutical Science VALENSI Jurnal Ilmiah Aplikasi Isotop Radiasi Alchemy : Journal of Chemistry JURNAL BIOLOGI INDONESIA Jurnal Kimia Terapan Indonesia JSFK (Jurnal Sains Farmasi & Klinis) Molekul: Jurnal Ilmiah Kimia Indonesian Journal of Halal Research Livestock and Animal Research Kimia Padjadjaran
Sandra Hermanto
Program Studi Kimia Fakultas Sains Dan Teknologi Universitas Islam Negeri Syarif Hidayatullah Jakarta
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Karakterisasi Produk Biosolubilisasi Lignit oleh Kapang Indigenus dari Tanah Pertambangan Batubara di Sumatera Selatan Sugoro, Irawan; Hermanto, Sandra; Sasongko, Dwiwahju; Indriani, Dea; Aditiawati, Pingkan
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.878 KB) | DOI: 10.14203/jbi.v7i2.3116

Abstract

ABSTRACTCharacterization of Lignite Biosolubilization Products by Indigenous Moulds from Soil ofCoal Mining in South Sumatera. Biosolubilization of coal is a potential technology of convertingsolid coal to liquid fuel and chemicals at ambient condition. Our previous research hassuccessfully isolated four moulds from soil at coal mining - South Sumatera and has potency aslignite biosolubilization agent, i.e. T1, T2, T4, T5. The objective of this research was to characterizeof lignite biosolubilization products by four isolates. The method used was sub-mergedculture. Cultivation medium was MSS+ (minimal salt + sucrose 0,1% + yeast extract 0,01% +lignite 5 %). Incubation was conducted at room temperature for 28 days. The result showed thatall indigenos moulds have different ability in lignite biosolubilization. The highestbiosolubilization occurred after 7 days of incubation belonging to T1 isolate. However, GC-MSanalysis showed the largest percentage of hydrocarbon compound which equivalent to gasolineand diesel was T5 after 7 days of incubation.Key words: Biosolubilization, lignite, moulds, coal.
ACE Inhibitory and Antioxidative Bioactive Peptides Derived from Hydrolyzed Soy Milk Hermanto, Sandra; Septiana, Annisa; Putera, Deni K; Hatiningsih, Fitriah; Muawanah, Anna
Molekul Vol 14, No 1 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.59 KB) | DOI: 10.20884/1.jm.2019.14.1.506

Abstract

Soy milk is a soybean processed product rich in protein as well as sources of bioactive peptides. Bioactive peptides defined as specific protein fragments that have a positive impact on body functions and conditions and may ultimately influence health. This study was conducted to explore the potential of hydrolyzed soy milk as a source of antioxidative and antihypertensive bioactive peptides through enzymatic hydrolysis. The initial treatment of soy milk protein was acidic precipitation with hydrochloric acid. Furthermore, protein precipitate was hydrolyzed using pepsin proteolytic enzyme with an enzyme: substrate ratio (1:5, 1:10 and 1:20). Protein hydrolysis was carried out for 0?48 hours at 37 °C in an acetate buffer pH 4.5. The soy milk protein hydrolysates were subjected to determination of % DH (Degree of Hydrolysis) and protein profile by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). The antihypertensive assay was carried out by in vitro inhibition of the ACE enzyme (Angiotensin Converting Enzyme) and antioxidative activity assay using the DPPH method. The results showed that the optimum conditions for hydrolysis of soy milk were obtained at 40 hours with a % DH value of 53.24% in enzyme ratio 1:20 and the highest antihypertensive activity was obtained from 48 hours hydrolysis with % ACE inhibition value of 79.31%. The highest antioxidative activity of bioactive peptide was obtained at hydrolysis 48 hours with IC50 69.1034 µg / ml. After fractionated and LCMS characterized it was obtained 2 bioactive peptides with molecular weights of 8.954 and 2,696 kDa. These bioactive peptides from hydrolyzed of soymilk might be potential as an antihypertensive agent and reduce oxidative stress.
Virtual Screening Peptida Bioaktif Antihipertensi dari Hidrolisat Kasein Susu Kambing Etawa Hermanto, Sandra
ALCHEMY Vol 5, No 2 (2016): ALCHEMY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.326 KB) | DOI: 10.18860/al.v5i2.3671

Abstract

Penapisan peptida bioaktif dari hidrolisat kasein susu kambing Etawa yang berpotensi sebagai obat antihipertensi berdasarkan kajian in silico telah dilakukan. Protein yang digunakan adalah α-S1-kasein prekursor [Capra hircus] NCBI Reference Sequence: NP_001272624.1, α-S2-kasein prekursor [C. hircus] NCBI Reference Sequence: NP_001272514.1, β-kasein [C. hircus] NCBI Reference Sequence: AAA30906.1 dan κ-kasein prekursor [C. hircus] NCBI Reference Sequence: NP_001272516.1. Perancangan struktur peptida bioaktif dilakukan melalui simulasi hidrolisis enzimatik dengan menggunakan 3 jenis enzim proteolitik (tripsin, kimotripsin dan pepsin) dan dilanjutkan dengan preparasi struktur 3D ligan hasil pemotongan secara in silico. Virtual screening terhadap fragmen peptida dilakukan melalui penentuan nilai drug likeness dan protease inhibitor. Dari 104 fragmen peptida diperoleh 10 kandidat peptida bioaktif yang dilakukan simulasi molecular docking dengan mengeksplorasi daya inhibisi fragmen melalui perhitungan nilai (∆Gbinding) dan interaksi antara kandidat peptida bioaktif dengan residu asam amino pada sisi aktif enzim ACE (Angiotensin Converting Enzyme). Sebagai kontrol positif digunakan lisinopril yang merupakan inhibitor ACE komersil. Hasil penelitian menunjukkan dari 10 kandidat peptida bioaktif terdapat 6 peptida yang diduga bersifat antihipertensi dengan nilai ∆Gbinding yang lebih rendah dari kontrol positif (lisinopril). Keenam peptida tersebut diharapkan dapat berfungsi sebagai obat alternatif antihipertensi.
Differentiation of Bovine and Porcine Gelatin Based on Spectroscopic and Electrophoretic Analysis Hermanto, Sandra; Sumarlin, La Ode; Fatimah, Widya
Journal of Food and Pharmaceutical Sciences Vol 1, No 3 (2013): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.524 KB) | DOI: 10.14499/jfps

Abstract

This study was conducted to explore the differentiation of bovine and porcine gelatins before and after pepsin hydrolysis based on peptide pattern from spectroscopic and electrophoretic analysis due to development of the halal food products analysis. In this study, pepsin (EC 3.4.23.1) was used to hydrolyze the two sources of gelatin with consideration to its ability to digest up to 20% of ingested amide bonds by cleaving preferentially after the N-terminal of aromatic amino acids such as phenylalanine, tryptophan, and tyrosine. In this study, we expect to produce the fragment of gelatins with differentiation in relative molecular weights. Gelatins fragments then analyzed by UV-Vis and FTIR spectroscopy to characterize the functional groups on each source of gelatins, followed by SDS-PAGE (Sodium Duodecylsulphate Polyacrylamide Gel Electrophoresis) to identify the molecular weight of the resulting fragments. In UV-Vis spectroscopy, both gelatin source before and after hydrolysis had different absorbance at 229 nm and 240 nm showing the proportion of C=O amida and differences in two-dimensional conformation of the peptide. In terms of FTIR spectra, both gelatin have wavenumber at 3300-3400 cm-1 (NH stretching), 1600 cm-1 (C=O stretching, amida), 1500 cm-1 (C-N stretching), and at 620-767 cm-1 (OCN bending). This indicates that the relative amino acid compositions from two sources of gelatins were relatively different. In contrast, SDS-PAGE analysis does not give a real differentiation, except for porcine gelatin, that fragments which on 2 hour incubation show two peptide fragments with molecular sizes below 36,2 kDa and 28.6 kDa.
ELECTRICAL STUNNING AND PRE-SLAUGHTERING TIME INTERVAL EFFECT IN EXPRESSION OF HEART BROILER CHICKENS PROTEIN Hermanto, Sandra
Journal of Food and Pharmaceutical Sciences Vol 3, No 3 (2015): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.468 KB) | DOI: 10.14499/jfps

Abstract

Pre-slaughtering process by transporting broiler chickens from farm to slaughterhouse and treatment of electrical stunning could be one of halal critical point. Stressing on chickens could be trigger a specific protein expression or increase its expression as a result of changes in metabolism that occurs. The purpose of this study was to identify the effect of treatment of electrical stunning and the pre-slaughtering interval time on broiler chicken liver protein expression through the analysis of protein profile by SDS-PAGE. Experiments conducted on 7 broiler chickens aged 35 days were given pre-slaughter treatment are different. Treatments include electrical stunning of 0 V, 25 V and 250 V as well as the pre-slaughtering time interval 0 hours, 2 hours, 2 hours + 12 hours (fasting) and 2 hours + 12 (fasting) with electrical stunning 250 V. Each of samples (chicken liver protein) isolated by homogenized in Trs-HCl buffer and the protein concentration determined by Lowry method. Identification of the protein profile was done by observing the differences in the intensity of the electrophoresis results followed by densitometry analysis. The results showed significant differences in outcomes electrical stunning treatment as indicated by the appearance of protein bands at around 109 kDa and do not appear on other treatments. The difference is predicted from particular protein expressed induced by cell metabolism responsive in chicken liver cell.
KARAKTERISASI PRODUK BIOSOLUBILISASI LIGNIT OLEH KAPANG INDIGENUS DARI TANAH PERTAMBANGAN BATUBARA DI SUMATERA SELATAN Sugoro, Irawan; Hermanto, Sandra; Sasongko, Dwiwahju; Indriani, Dea; Aditiawati, Pingkan
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3116

Abstract

ABSTRACTCharacterization of Lignite Biosolubilization Products by Indigenous Moulds from Soil ofCoal Mining in South Sumatera. Biosolubilization of coal is a potential technology of convertingsolid coal to liquid fuel and chemicals at ambient condition. Our previous research hassuccessfully isolated four moulds from soil at coal mining - South Sumatera and has potency aslignite biosolubilization agent, i.e. T1, T2, T4, T5. The objective of this research was to characterizeof lignite biosolubilization products by four isolates. The method used was sub-mergedculture. Cultivation medium was MSS+ (minimal salt + sucrose 0,1% + yeast extract 0,01% +lignite 5 %). Incubation was conducted at room temperature for 28 days. The result showed thatall indigenos moulds have different ability in lignite biosolubilization. The highestbiosolubilization occurred after 7 days of incubation belonging to T1 isolate. However, GC-MSanalysis showed the largest percentage of hydrocarbon compound which equivalent to gasolineand diesel was T5 after 7 days of incubation.Key words: Biosolubilization, lignite, moulds, coal.
Karakterisasi Produk Biosolubilisasi Batubara Oleh Kapang T4 Hasil Isolasi dari Tanah Pertambangan Tanjung Enim Sumatera Selatan Irawan Sugoro; Sandra Hermanto; D Indriani; P Aditiawati; D Sasongko
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.1, November 2010
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (591.29 KB) | DOI: 10.15408/jkv.v2i1.231

Abstract

Biosolubilisasi batubara adalah teknologi yang memiliki potensi untuk mengubah batubara padatmenjadi bahan bakar cair/senyawa kimia dengan bantuan mikroorganisme. Tujuan dari penelitianini adalah untuk mengkarakterisasi produk biosolubilisasi batubara oleh kapang T4 hasil isolasidari tanah di area pertambangan batubara Tanjung Enim Sumatera Selatan. Hasil penelitianmenunjukkan bahwa terjadi interaksi antara kapang T4 dengan batubara yang dilihat dari adanyakolonisasi miselia di permukaan batubara. Kadar asam humat mengalami peningkatan hingga harike-7, sedangkan kadar asam fulvat terus meningkat hingga hari ke-28. Analisa gugus fungsibatubara hasil biosolubilisasi dengan FTIR memperlihatkan terjadinya perubahan strukturbatubara yang didominasi oleh penurunan serapan C=C aromatic dan C-O fenolik.. Karakterisasiproduk biosolubilisasi batubara oleh kapang T4 dengan GC-MS menunjukkan terbentuknyasenyawa-senyawa baru dan senyawa yang mengalami peningkatan antara lain naftalena dansenyawa alkana rantai pendek.
Profil dan Karakteristik Lemak Hewani (Ayam, Sapi dan Babi) Hasil Analisa FTIR dan GCMS Sandra Hermanto; Anna Muawanah; Rizkina Harahap
Jurnal Kimia Valensi Jurnal Valensi Volume 1, No.3, November 2008
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3137.171 KB) | DOI: 10.15408/jkv.v1i3.219

Abstract

Telah dilakukan penelitian tentang analisa profil dan karakteristik beberapa lemak hewani sebagaistudi pendahuluan dalam rangka pengembangan metode analisa kehalalan pangan. Samplingdilakukan terhadap tiga jenis sampel jaringan lemak hewani yang meliputi lemak ayam, lemak sapidan lemak babi. Sampel jaringan lemak ayam dan sapi diperoleh dari pasar tradisional sedangkansampel jaringan lemak babi diperoleh dari Rumah Pemotongan Hewan di daerah Jakarta Timur.Masing-masing sampel jaringan lemak diekstrak dengan pemanasan langsung dan selanjutnyadianalisa sifat fisikokimianya meliputi bobot jenis, indeks bias, titik leleh, bilangan asam, bilanganiod dan bilangan penyabunan. Analisa lebih lanjut dilakukan dengan metode FTIR (FourierTransform Infra red) dan GCMS (Gas Chromatography Mass Spectromtery) untukmengidentifikasi spesifitas masing-masing lemak berdasarkan pola serapan gugus fungsi dankomposisi asam lemaknya. Hasil analisa sifat fisikokimia yang diperoleh menunjukkan bahwa tidakterdapat perbedaan yang cukup signifikan untuk masing-masing sampel lemak kecuali untuk titikleleh, bilangan iod dan bilangan penyabunannya. Hasil analisa FTIR menunjukkan adanyaperbedaan pola serapan yang khas pada daerah 3010, 1110-1095 dan 975-965 cm-1 yangmerepresentasikan tingkat perbedaan komposisi asam lemak pada masing-masing sampel. Hal inidiperkuat dengan hasil analisa GCMS yang membuktikan adanya perbedaan kandungan SFA(saturated fatty acid), MUFA (monounsaturated fatty acid) dan PUFA (polyunsaturated fattyacid) pada ketiga sampel.
Profil Protein Escherichia coli Hasil Inaktivasi Iradiasi Gamma Sebagai Bahan Vaksin Mastitis Sandra Hermanto; I Sugoro; Ikmalia Ikmalia
Jurnal Kimia Valensi Jurnal valensi Volume 1, No.2, Mei 2008
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2021.146 KB) | DOI: 10.15408/jkv.v1i2.214

Abstract

Penelitian ini bertujuan untuk mengetahui profil protein Escherichia coli hasil iradiasi gammasebagai bahan vaksin mastitis inaktif. Kultur sel (108 sel/ml) diinaktivasi dengan dosis radiasisebesar 600, 700, 800, 900 dan 1000 Gy. Kultur sel dianalisis kandungan protein intraselulardan ekstraselular dengan metode Lowry. Profil protein dianalisis dengan menggunakan SDSPAGEdengan konsentrasi gel 10% dan standar berat molekul kisaran 10 – 220 kDa. Hasilpercobaan menunjukkan bahwa tidak terjadi perubahan kadar protein intraselular danekstraselular secara signifikan dengan semakin tingginya dosis iradiasi, demikian pula denganjumlah pita protein, tetapi intensitas setiap pita menunjukkan adanya perbedaan. Perbedaanintensitas protein tertinggi terjadi pada dosis 900 dan 1000 Gy. Hal ini menunjukkan bahwairadiasi gamma mampu menginaktivasi sel bakteri tetapi tidak merusak protein secara totalsebagai salah satu bahan antigen vaksin.
Pengaruh Kosubstrat Tapioka terhadap Serapan Mineral Jamur Tiram Putih Sandra Hermanto; Irawan Sugoro
Jurnal Kimia Valensi Jurnal Valensi Volume 1, No.5, November 2009
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2757.509 KB) | DOI: 10.15408/jkv.v1i5.305

Abstract

Co-Authors Ahmad, Syadzwina Nurdzakiyyah Aldi Octavio Anna Muawanah Arief Adhari Asih Widi Wisudawati Asih Widi Wisudawati Azrifitria ., Azrifitria Chitta Putri Novianti Cut Dhien K Meutia D Indriani D Sasongko Dadang Sudrajat Dede Sukandar Dedi Ansori Dwiwahju Sasongko Eddy Jusuf Eka Rizki Amelia Eka Rizki Amelia Emi Lestari Emi Lestari Eva Silvia Fahrur Rahman Saputra Faiz Nur Faiqoh Farhat Etriya Fitri, Dhea Ameliana Hatiningsih, Fitriah Hilman Affandi I Sugoro Ikmalia Ikmalia Imamah Al Mabrur Imamah Al Mabrur Indriani, Dea Indriani, Dea Irawan Sugoro Irawan Sugoro Irawan Sugoro La Ode Sumarlin M. Hero Shiddiqi Maya Ina Sholaikah Mirzan T Razzak Muhamad Zaenudin Muhammad Ihda Hamlu Liwaissunati Zein Nana Mulyana Nurgraha, Auliyah Aisyah P Aditiawati PINGKAN ADITIAWATI Prita Wardhani Priyambodo Priyambodo Putera, Deni K Rizkina Harahap Robiatul Adawiyah Septiana, Annisa Septyani Nurichawati Shintia Nugrahini Wahyu Hardani Sri Suci Mulyani Susi Kusumaningrum Syadzwina Nurdzakiyyah Ahmad Syifa Rya Haryati Tarso Rudiana, Tarso Teguh Wahyono Thamzil Las Tiah Maharani Tri Retno Diah Larasati Wahidin Teguh Sasongko Widya Fatimah Yulyani Nur Azizah Yunida Maharani Zein, Muhammad Ihda Hamlu Liwaissunati Zilhadia Zilhadia