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All Journal Jurnal Farmasi Indonesia Jurnal Insan Farmasi Indonesia
Bayu Herdi Al Huda
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Physics

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DOCKING MOLEKULER SENYAWA B-KAROTEN DALAM TANAMAN KELOR (Moringa Oleifera L.) SEBAGAI PENGHAMBAT ENZIM TIROSINASE DENGAN AUTODOCK – VINA Bayu Herdi Al Huda; Nining Sugihartini; Hari Susanti; Dwi Utami
Jurnal Insan Farmasi Indonesia Vol 3 No 2 (2020): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v3i2.540

Abstract

Hydroquinone has been used in cosmetics because of its whitening activity. In previous studies, B-carotene in Moringa plants was also known as an inhibitor of the tyrosinase enzyme. It is necessary to know how the interaction mechanism of B-carotene with tyrosinase (5M8N) and which compounds between hydroquinone and B-carotene provide computationally better activity as whitening. Tyrosinase was prepared using Discovery Studio Visualizer. Ligands were prepared using Autodock 4.2. Autodock-Vina is used for ligand docking between proteins. The result is the binding affinity (kcal/mol) of the ligand to protein. Visualization of docking between ligands and proteins using the Ligplot + Program with a 1 year license. Media used for the docking process is a computer with an Intel Core i7-3770 CPU with a speed of 3.40 GHz 8 cores, 1920x1080p resolution, VGA NVIDIA GeForce GTX 750, 8 GB RAM, Windows 8 64-bit. The docking results showed that the binding affinity of B-carotene to tyrosinase was -11.2 while hydroquinone with tyrosinase was -5.4 with RMSD 0. The results of visualization showed that B-carotene binds more amino acid receptors than hydroquinone. B-carotene in moringa has been shown to be active not only in wet laboratories, but also in dry laboratories.
The Purification effect on Organoleptic Profile, Yield, Total Phenol and Total Flavonoids from 96% Ethanol Extract of Moringa (Moringa oleifera. L) leaves. Bayu Herdi Al Huda; Hari Susanti; Nining Sugihartini
Jurnal Farmasi Indonesia Vol 17 No 2 (2020): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/jfi.v17i2.983

Abstract

Purification extract ethanol 96% of Moringa dry leaves was carried out to increase the active substance content and to obtain a more attractive color. The purpose of this study was to determine the type of solvent in the fractionation which provides a higher active substance content than the extract. This research begins with maceration of dried Moringa leaves with 96% ethanol. The filtrate that has been dried then purified. Purification begins with dissolving the extract in hot distilled water with a ratio of 1:10, after that ethyl acetate or n-hexane is added to the same amount as hot distilled water in a separating funnel, shaking it. The ethyl acetate or n-hexane phase is taken then the filtrate obtained is combined. The 96% ethanol extract (E1), ethyl acetate (E2) and n-hexane (E3) were then evaluated with parameters of organoleptic, % yield, total phenol, and total flavonoids. Data were analyzed statistically one way ANOVA with confidence level of 95%. The measurement results for E1, E2, and E3 in the yield were 15.81%;22.18%;20%, the total phenol was 28.94±0.54mgGAE/g; 29.44±0.93mgGAE/g; 11,41±0,12mgGAE/g, and total flavonoids 3,19±0.13%; 9,92±0.06%; 5.81±0.18% respectively. The consistency, smell and taste, all are identical, while in color, E2 is more yellowish green and brighter, making it more attractive. Statistically, there were significant differences between E1, E2, and E3 in total phenols and flavonoids. Thus it can be concluded that E2 significantly higher total phenol and flavonoids than E1 and E3.
Co-Authors Hari Susanti Muhammad Yusron Maulana El-Yunusi Nining Sugihartini