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All Journal Jurnal Ilmu Pertanian Indonesia Indonesian Journal of Agricultural Science BERITA BIOLOGI

Ahmad Dadang

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Author-ID : 248587

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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IMPROVEMENT OF EARLY MATURITY IN RICE VARIETY BY MARKER ASSISTED BACKCROSS BREEDING OF Hd2 GENE Fatimah, Fatimah; Prasetiyono, Joko; Dadang, Ahmad; Tasliah, Tasliah
Indonesian Journal of Agricultural Science Vol 15, No 2 (2014): October 2014
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Early-maturing and high-yielding rice variety is very useful for increasing rice production in Indonesia. The aim of this research was to develop new lines of Indonesian rice containing Hd2 gene using Code variety as a recipient parent and Nipponbare variety as a donor parent through targetted MAB approach using RM1362 and RM7601 in chromosom 7 for foreground selection. After two generations of backcrossing, the positive alleles of Hd2 gene from Nipponbare had successfully trans-ferred into Code. The plant number CdNp_29 in BC2F2 popula-tion had the highest genome recovery of 82.7%. The twelve BC2F3 plants were selected for self-pollination to generate BC2F4. These selected lines that carried the Hd2 gene were screened in the greenhouse for the evaluation of heading date and agronomic traits. All improved lines had Hd2 gene similar to the donor parent Nipponbare. The heading date of the breeding lines ranged from 73 to 89 days (Code 85 days) or fill the third criterion of rice maturity that is 103-104 days compared to Code of 116-119 days, whereas their agronomic performances were similar with that of Code. Application of MABc for im-proving rice early maturity has accelerated the development and selection in early generation of superior lines having genetic background of Code. It is expected that the newly developed lines of Code will be utilized to increase rice production in Indonesia.

SELEKSI BERBANTUKAN MARKA MOLEKULER UNTUK PEMBENTUKAN CALON VARIETAS PADI TURUNAN ESENSIAL SITU PATENGGANG Yuriyah, Siti; Utami, Dwinita Wikan; Nurani, Siti; Nasution, Anggiani; Lestari, Puji; Dadang, Ahmad; Suwarno, Suwarno
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The utilization of molecular marker technology for Blast (Pyricularia grisea) resistance in rice breeding could accelerate and improve the precision of selection. This study aimed to identify and to select the BC3F2 progenies from Situ Patenggang and the opted Blast Monogenic Lines based on their resistance to blast disease in green house and field using molecular characterization. A total of 200-300 BC3F2 population strains resulted in 4 crosses between Situ Patenggang varieties and Monogenic Varieties IRBLta2-Re, IRBLkp-k60, IRBLi-F5 and IRBLa-A were used as total genetic material in the study. Blast resistance assay was initially carried out in a greenhouse and further selection was conducted in the endemic blast area, Sukabumi. The selected Molecular marker was STS (Sequence Taq Sites) marker for foreground selection and 384-SNPs (Single Nucleotide Polymorphism) chip for background selection. The number of lines from each cross was selected by greenhouse assay i.e: 25 lines derived from Situ Patenggang/IRBLta and Situ Patenggang/IRBLkp, 21 plants for crossing Situ Patenggang/IRBLi, and 22 plants for Situ Patenggang/IRBLa-A. The results of field experiment revealed that blast resistance response of the selected lines was varied from 0 to 5. Lines of foreground were successfully selected using STS markers specified for Pii, Pita, Pikp and Pia genes. As for the background selection by SNPs markers, some lines carried the recurrent parent genetic background, Situ Patenggang. Overall, 20 resistance lines that harbored the target genes and Situ Patenggang background were obtained. Further observation was entailed to these twenty selected lines in order to attain promising lines candidate for blast resistance.

PERBAIKAN PADI (Oryza sativa L.) VARIETAS CIHERANG UNTUK SIFAT UMUR GENJAH DAN PRODUKSI TINGGI MENGGUNAKAN MARKA MOLEKULER Prasetiyono, Joko; Tasliah, Tasliah; Dadang, Ahmad; Fatimah, Fatimah
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The improvement of rice (Oryza sativa L.) Ciherang variety for early maturity and high production traits was carried out by Marker Assisted Backcrossing (MAB) method using Nipponbare as donor parent. The foreground selection of Hd2 gene was laid on flanking markers of RM1362 and RM7601 in QTL region. The selection process of F1 to BC2F2 plants were based on molecular markers and agronomic characters. The BC2F3 plants were challenged to bacterial leaf blight to know their resistance in this hybridization. The results indicated that the foreground and background selection were not sufficient as selection tools therefore they would be more accurate if assosiated with agronomic characters. Four selected lines derived from Ciherang x Nipponbare crossed (BC2F3 plant # 283, 307, 373, and 462) could be promising lines with early maturity and high productioncompared to Ciherang. Selected BC2F3 lines flowered earlier than original Ciherang up to 7-10 days, while the yield increasing was 3.55 to 9.2% higher based on weight of filled grains/plant, and from 3.58 to 19.39% higher based on the number of filled grain/plant. However, all of BC2F3 lines were not resistant to bacterial leaf blight attack.

Evaluasi Mutu Fisikokimia Beras Hasil Persilangan antara Empat Padi Varietas Unggul dan Padi Liar (Oryza glaberrima dan O. rufipogon) Yuriyah, Siti; Purwanto, Oky Dwi; Afza, Higa; Dadang, Ahmad; Masyuri, Agus; Galurina, Rina Siti; Utami, Dwinita Wikan
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 3 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.3.397

The physicochemical quality of rice is evaluated to ensure that it is fit for consumption and meets established quality standards. This study aims to analyze the physicochemical properties of rice strains resulting from crosses between four high-yielding varieties of rice and two wild rices so that the appropriate genotype is obtained for the development of quality rice. The research was conducted from November 2019 to March 2020 at the Quality Laboratory, Muara Experimental Garden, Rice Research Center, Bogor. The rice genotypes used were 22 strains resulting from crosses between high-yielding varieties of rice (Ciherang, Inpari 13, Inpari 10, and Situ Bagendit), and wild rice (Oryza glaberrima and O. rufipogon), 4 comparison varieties of crossbred elders, and two comparison varieties of organoleptic tests (Memberamo and IR42). Physical and chemical qualities were observed in terms of grain moisture content, grain density, broken skin rice yield, milled rice yield, head rice yield, rice length, rice shape, and rice liming, amylose content, gelatinization temperature, and organoleptic. The results showed that the strains 44-F-C21, 58-F-C21, and 59-F-C21 provided fairly good rice quality with head rice yields above 80% and higher than their respective elders (Situ Bagendit and Ciherang), and water content below 14%. These strains had characteristic characteristics, namely medium rice form, amylose content of 26.38%, and alkali score of 2-3. Most of these genotypes also exhibited high gelatinization temperatures, medium rice texture, and savory rice flavors. The genotype selected from the results of crosses between high-yielding varieties of rice and wild rice has the potential to be developed because there are genetic improvements from its elders on several physicochemical characteristics of rice. Keywords: amylose content; gelatinization temperature; physicochemical properties; rice lines; wild rice

Co-Authors DWINITA WIKAN UTAMI fatimah Fatimah Galurina, Rina Siti Higa Afza, Higa Joko Prasetiyono Masyuri, Agus Nasution, Anggiani PUJI LESTARI Purwanto, Oky Dwi Siti Nurani Siti Yuriyah Suwarno Suwarno Tasliah Tasliah

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