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The Effect of Food Supplementation Program on Anthropometry, Hematology, and Parasites of Primary School Children Christin M. Santosa, Christin M. Santosa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 3 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.55 KB)

Background: Food supplementation is problem solving efforts during multidimensional crisis.Objectives: This research was carried to evaluate further the advantages of the food supplementation in the primary school children towards their physical development and health status.Methods: Students with completely participation on these research and their parents agreed to informed consent were taken as inclusion criteria. The subjects of this experiment were sixth grade school children and divided into 2 groups i.e 25 control and 31 treated groups. The treatment group was given food supplementation consisting of one boiled egg and one glass of green-peas porridge (220 ml) each, three times a week for 10 weeks, whereas the control group was not given any. Observations were carried out five times: at 0, 3, 7, 10, and 13 weeks. The results were analized by the Student-t test.Results: The results showed significant increases in body weights (p0.05) between control and treatment groups neither for the boys nor girls. And until the last week faeces samples, it is never detected the situation where it is free from worm investation and protozoa infection either for control or treatment group.Conclusion: The food supplementation was apparently adequate to meet the optimal caloric and proteins requirements of these children, so that could improve physical development and health status as shown by the increased anthropometric measurements, decreasing parasite infection and steady hematological picture.Key words: food supplementation - anthropometry - hematology - parasites - primary school children

Klasterisasi Staphylococcus aureus Resisten Neutrofil Berdasar Assesory Gene Regulator Santosa, Christin Marganingsih; Lestari, Fajar Budi; Widayanti, Rini; Salasia, Siti Isrina Oktavia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.50653

Staphylococcus aureus is recognized worldwide as a major pathogen causing subclinical intramammary infections in dairy cows and food poisoning due to its ability to produce enterotoxin. The study aimed to identify enterotoxins of S. aureus and clustering the enterotoxins based on assessory gene regulator (agr). Virulence of S. aureus to the host was characterized based on the response of polymorphonuclear cells to the infection. Twelve S. aureus could be isolated from milk cows in central of dairy farming in Sumedang West Java. The identification of S. aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene. The sensitivity test against antibiotics revealed that some isolates of S. aureus were resistant to penicillin and methycillin. By PCR amplification one or more staphylococcal enterotoxin genes could be observed five genes in combinations of sea (216 bp), seb (478 bp), seh (375 bp), sei (576 bp), and sej (142 bp). Clustering of S. aureus based on the assesory gene regulator could be grouped into 4 clusters for agr1 (1 isolat), agr2 (2 isolates), in combination for agr1 and agr2 (1 isolate), and for non agr (2 isolates). Based on the response of polymorphonuclear cell in vitro and in vivo assays, revealed that S. aureus strain I-2 (agr1 cluster) and P1 (agr1+agr2 cluster) were more resistant to polymorphonuclear cells and could survive intracellularly, indicated that these strains could be used as proper candidates to develop dignostic tool based on agr against staphylococcal mastitis.

Efek Ekstrak Daun Bangun-Bangun (Coleus ambonicus, L.) pada Aktivitas Limfosit Tikus Putih Christin Marganingsih Santosa; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Efek Ekstrak Air Daun Bangun-Bangun (Coleus ambonicus, L) pada Aktivitas Limfosit Tikus Putih Christin Marganingsih Santosa; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Kajian Kliniko-patologik dan Antimikroba Ekstrak Biji Alpukat (Persea Americana Mill) Christin Marganingsih Santosa; Imron Rosyadi; Dinar Arifianto; Salasia Isrina Oktavia Siti
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The phytochemical components of avocado seeds (Persea americana Mill) consist of alkaloids, flavonoids, tannins and saponins, so that they have the potential as antibacterials. The aim of this study was to determine the ability of Persea americana Mill extract to inhibit the growth of Staphylococcus aureus through inhibition zone observation and the ability to accelerate wound healing infected by S. aureus through observation of wound closure and cytopathology. Persea americana Mill were extracted using maceration method with 70% ethanol. The activities of this extract were tested by diffusion disc agar method. Biomedical tests were carried out on anaestetic Wistar rats whose were S. aureus infected wounds and treated with Persea Americana Mill extract. Based on the results of the antimicrobial test, avocado seed extract has a inhibitory effect on the growth of S. aureus at a concentration of 6.25% and best at a concentration of 100% compared to the control. Tests in experimental rats showed the effect of wound healing on the 2rd day after giving the extract Persea americana Mill ointment, faster than the control, which was need 4 days of wound closure. Cytopathology results showed that leukocyte activity was more prominent in rats treated with avocado seed extract compared to control rats. Avocado seeds as byproducts have the potential to be new herbal medicines that can be used as antimicrobials of S. aureus which have been confirmed to be resistant to various antibiotics.

The Adaptation of Small Intestine Nitregic Myenteric Neurons on Rats (Rattus norvegicus) to High Fat Diet Amelia Hana; Sarmin Sarmin; Claude Mona Airin; Christin Marganingsih Santosa; Pudji Astuti
Buletin Peternakan Vol 43, No 2 (2019): BULETIN PETERNAKAN VOL. 43 (2) MAY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i2.38810

High fat diet can result in the loss of nitrergic neurons in the myenteric plexus. The study aimed at finding out the effect of high fat diet on the adaptation of nitrergic nerve of rat intestine. It used 15 male rats (Rattus norvegicus) of a month of age with mean body weight of 53.73 gr. The rats were adapted for 7 days to individual cages with ad libitum feeding. After random adaptation, all of the rats were assigned to 3 groups of five rats, namely K-7, K-10, and K-13 groups. Feed and drinking water were given ad libitum. The treatment of the high fat diet lasted for 7 weeks. After the treatment, all of the rats were fasted for 12 hours and then killed. Subsequently, small intestine segments (duodenum, jejunum, and ileum) were taken. The intestinal segments were prepared by using NADPH-d histopathological technique to determine the morphometric changes of nitrergic myenteric neurons. During the treatment the rats were weighed every week and at the end of the study orbitalis vein blood measurement was carried out to see its glucose, cholesterol and cholecystokinin (CCK) plasma levels. The data of body weight, glucose, cholesterol, CCK levels, the total number of the nippergenic myenteric segments of the small intestine were statistically analyzed using Anova. The results of the study showed that the treatment of 7% to 13% fat diets for 7 weeks did not indicate any weight gain and any increase in cholescystokinin level, and any decrease in glucose level. However, it indicated significant increase in cholesterol level. The treatment of 10% and13% fat diets increased the total number of neurons in the jejunum and the ileum. Thus, it was concluded that the treatments of the high-fat diet of the rats (K-7, K-10, and K-13) for 7 weeks had significant effect on the adaptation of the neurons of the jejunum and the ileum.

The Effect of Food Supplementation Program on Anthropometry, Hematology, and Parasites of Primary School Children Christin M. Santosa Christin M. Santosa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 3 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.55 KB)

Background: Food supplementation is problem solving efforts during multidimensional crisis.Objectives: This research was carried to evaluate further the advantages of the food supplementation in the primary school children towards their physical development and health status.Methods: Students with completely participation on these research and their parents agreed to informed consent were taken as inclusion criteria. The subjects of this experiment were sixth grade school children and divided into 2 groups i.e 25 control and 31 treated groups. The treatment group was given food supplementation consisting of one boiled egg and one glass of green-peas porridge (220 ml) each, three times a week for 10 weeks, whereas the control group was not given any. Observations were carried out five times: at 0, 3, 7, 10, and 13 weeks. The results were analized by the Student-t test.Results: The results showed significant increases in body weights (p0.05) between control and treatment groups neither for the boys nor girls. And until the last week faeces samples, it is never detected the situation where it is free from worm investation and protozoa infection either for control or treatment group.Conclusion: The food supplementation was apparently adequate to meet the optimal caloric and proteins requirements of these children, so that could improve physical development and health status as shown by the increased anthropometric measurements, decreasing parasite infection and steady hematological picture.Key words: food supplementation - anthropometry - hematology - parasites - primary school children

Phytochemical compounds and the effect of ‘bangunbangun’ leaves (Coleus amboinicus, L.) water extract on phagocytosis activity of neutrophil cell rat (Rattus norvegicus) Christin Marganingsih Santosa; Triana Hertiani
Indonesian Journal of Pharmacy Vol 16 No 3, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Traditional medicine has been recognized and widely used in Indonesia for healthy care or alternative medication in certain diseases. However, without scientific evidence, traditional medicine of empirical evidence cannot be used for modern health medication. This research was designed to find out the mains compound despite of Bangun-bangun aqueous extract from Kaliurang district using phytochemistry analysis such as Thin Layer Chromatography method and determine the effect on neutrophil cell activities by observing their phagocytosis. The result showed that the main compounds of Bangun-bangun leaves from Kaliurang district are polyphenol, saponin, flavonol glycoside and essential oil. Administration of the extract with the doses of Bangun-bangun leaves treatment 19,0 g/kg bw/oral/day (group A) and 31,5 g/kg bw/oral/day (group B) increased neutrophil cell phagocytosis up to 50% and 60 %, respectively, compared to those of controls which is 10% during 30 days treatment. Neutrophil cell phagocytosis in group B and C capability increase up to 80% (p

Analisis kadar fibrinogen sebagai biomarker diabetik pada tikus wistar yang diinduksi streptozotocin Imron Rosyadi; Ella Romadhona; Ajeng Tyas Utami; Yayik Nur Hijrati; Christin Marganingsih Santosa
ARSHI Veterinary Letters Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Penyakit diabetes mellitus adalah serangkaian proses penyakit yang bermula dari kerusakan jaringan terutama di pankreas dimana awalnya ditandai dengan munculnya protein fase akut. Pada mamalia, konsentrasi protein fase akut yang dominan adalah serum amyloid A protein, C-reaktive protein dan fibrinogen. Penelitian ini bertujuan untuk mencari korelasi protein fase akut fibrinogen untuk digunakan sebagai biomarker pada tikus diabetes yang diinduksi streptozotocin. Tikus yang digunakan adalah tikus Wistar jantan sebanyak 20 ekor, umur sekitar 2 bulan dengan berat badan 180-250 gram. Tikus dibagi menjadi dua kelompok, masing-masing kelompok 10 ekor tikus sebagai kelompok perlakuan (I) dan kelompok kontrol (II). Kelompok I dipuasakan selama 24 jam kemudian diinjeksi streptozotocin 1 kali dengan dosis 40 mg/kg bb yang dilarutkan dalam bufer sodium sitrat 0,1 M pH 4,0. Tikus diambil darahnya pada jam ke-0, 6, 12, 24, 48, 60, 72, 84 dan 96 post induksi diabetes untuk diperiksa kadar konsentrasi protein fase akut fibrinogen Hasil penelitian rata-rata kadar fibrinogen pada jam ke-0 dan ke-6 yaitu sebesar 0,57±0,06 mg/mL dan 0,60±0,35 mg/mL masih dalam range normal, kemudian mulai meningkat terus berturut-turut di jam ke-12 sebesar 0,6±0,4 mg/mL, jam ke-24 0.93±0,46 mg/mL, jam ke-36 1,1±0,1 mg/mL, jam ke-48 1,13±0,81 mg/mL, jam ke-60 1,17±0.40 mg/mL, jam ke-72 1,47±0,06 mg/mL, jam ke-84 1,6±0,1 mg/mL dan jam ke-96 1,8±0,1 mg/mL. Hasil dari penelitian ini menunjukkan protein fase akut fibrinogen dapat dijadikan sebagai salah satu marker pada tikus yang diinduksi diabetes mellitus.

Gambaran kadar gula darah tikus wistar diabetes hasil induksi streptozotocin dosis tunggal Imron Rosyadi; Ella Romadhona; Ajeng Tyas Utami; Yayik Nur Hijrati; Christin Marganingsih Santosa
ARSHI Veterinary Letters Vol. 2 No. 3 (2018): ARSHI Veterinary Letters - Agustus 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Penyakit diabetes mellitus adalah penyakit metabolik yang ditandai dengan adanya kenaikan kadar gula dalam darah. Penelitian ini bertujuan untuk melihat gambaran kadar gula darah tikus wistar yang diinduksi diabetes dengan streptozotocin. Tikus yang digunakan adalah tikus Wistar jantan sebanyak 20 ekor, umur sekitar 2 bulan dengan berat badan 180-250 gram. Tikus dibagi menjadi dua kelompok, masing-masing kelompok 10 ekor tikus sebagai kelompok perlakuan (I) dan kelompok kontrol (II). Kelompok I dipuasakan selama 24 jam kemudian diinjeksi streptozotocin 1 kali dengan dosis 40 mg/kg bb yang dilarutkan dalam bufer sodium sitrat 0,1 M pH 4,0. Tikus diambil darah pada jam ke-0, 6, 12, 24, 48, 60, 72, 84 dan 96 post induksi diabetes untuk diperiksa kadar gula darah. Hasil penelitian menunjukkan rata-rata kadar gula darah tikus masih dalam keadaan normal pada jam ke-0 sebesar 90,6±15,88 gr/dL dan jam ke-6 126±11,73 gr/dl lalu menurun pada jam ke-12 sebesar 48,4±6,26 gr/dL dan meningkat tajam sebesar 348,3±33,17 gr/dL pada jam ke-24 sampai akhir penelitian. Hasil dari penelitian ini menunjukkan streptozotocin dapat menginduksi tikus menjadi diabetes mellitus.

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